Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021015.01.T01 | XP_003606627.1 | 98.649 | 148 | 2 | 0 | 1 | 148 | 1 | 148 | 1.75E-97 | 287 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021015.01.T01 | Q1S9I9 | 98.649 | 148 | 2 | 0 | 1 | 148 | 1 | 148 | 3.94E-100 | 286 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021015.01.T01 | G7JTQ7 | 98.649 | 148 | 2 | 0 | 1 | 148 | 1 | 148 | 8.37e-98 | 287 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048220.01 | MsG0480021015.01 | 0.834963 | 2.211039e-56 | 2.665716e-53 |
| MsG0180002218.01 | MsG0480021015.01 | 0.806730 | 7.139247e-50 | 3.904006e-47 |
| MsG0180004538.01 | MsG0480021015.01 | 0.826546 | 2.556256e-54 | 2.404599e-51 |
| MsG0180004994.01 | MsG0480021015.01 | 0.833310 | 5.740732e-56 | 6.586070e-53 |
| MsG0180004995.01 | MsG0480021015.01 | 0.824124 | 9.568685e-54 | 8.399808e-51 |
| MsG0180005048.01 | MsG0480021015.01 | 0.870162 | 1.797402e-66 | 6.923079e-63 |
| MsG0180006133.01 | MsG0480021015.01 | 0.837559 | 4.840455e-57 | 6.311095e-54 |
| MsG0380014666.01 | MsG0480021015.01 | 0.840914 | 6.524312e-58 | 9.431939e-55 |
| MsG0380014684.01 | MsG0480021015.01 | 0.847288 | 1.271182e-59 | 2.248379e-56 |
| MsG0480020918.01 | MsG0480021015.01 | 0.894989 | 1.466712e-75 | 1.492928e-71 |
| MsG0480020919.01 | MsG0480021015.01 | 0.809961 | 1.460951e-50 | 8.697249e-48 |
| MsG0480020920.01 | MsG0480021015.01 | 0.878297 | 3.146326e-69 | 1.633978e-65 |
| MsG0480020921.01 | MsG0480021015.01 | 0.897960 | 8.464073e-77 | 9.796064e-73 |
| MsG0480020926.01 | MsG0480021015.01 | 0.904641 | 9.920251e-80 | 1.548756e-75 |
| MsG0480020927.01 | MsG0480021015.01 | 0.866000 | 3.924790e-65 | 1.302619e-61 |
| MsG0480020969.01 | MsG0480021015.01 | 0.864831 | 9.158846e-65 | 2.915530e-61 |
| MsG0480020970.01 | MsG0480021015.01 | 0.818838 | 1.590119e-52 | 1.203546e-49 |
| MsG0480020971.01 | MsG0480021015.01 | 0.862217 | 5.926742e-64 | 1.722468e-60 |
| MsG0480021015.01 | MsG0480021016.01 | 0.833487 | 5.185015e-56 | 5.979916e-53 |
| MsG0480021015.01 | MsG0480021048.01 | 0.841544 | 4.455991e-58 | 6.569166e-55 |
| MsG0480021015.01 | MsG0480021100.01 | 0.801022 | 1.095149e-48 | 5.175831e-46 |
| MsG0480021015.01 | MsG0480021252.01 | 0.827922 | 1.197257e-54 | 1.172213e-51 |
| MsG0480021015.01 | MsG0480021301.01 | 0.894420 | 2.508744e-75 | 2.491873e-71 |
| MsG0480021015.01 | MsG0480021302.01 | 0.885916 | 5.380883e-72 | 3.762056e-68 |
| MsG0480021015.01 | MsG0480022307.01 | 0.863713 | 2.045469e-64 | 6.262147e-61 |
| MsG0480021015.01 | MsG0480023003.01 | 0.830719 | 2.506010e-55 | 2.662330e-52 |
| MsG0480021015.01 | MsG0480023424.01 | 0.852276 | 5.132284e-61 | 1.067049e-57 |
| MsG0480021015.01 | MsG0480023426.01 | 0.850545 | 1.584228e-60 | 3.114035e-57 |
| MsG0480021015.01 | MsG0580025177.01 | 0.813087 | 3.056109e-51 | 1.976697e-48 |
| MsG0480021015.01 | MsG0580025504.01 | 0.846494 | 2.097015e-59 | 3.616176e-56 |
| MsG0480021015.01 | MsG0580025506.01 | 0.828873 | 7.057702e-55 | 7.103569e-52 |
| MsG0480021015.01 | MsG0580028955.01 | 0.834095 | 3.653558e-56 | 4.291325e-53 |
| MsG0480021015.01 | MsG0780036534.01 | 0.834700 | 2.575798e-56 | 3.080343e-53 |
| MsG0480021015.01 | MsG0780040620.01 | 0.855528 | 5.939866e-62 | 1.375430e-58 |
| MsG0480021015.01 | MsG0780041273.01 | 0.880095 | 7.279555e-70 | 4.048960e-66 |
| MsG0480021015.01 | MsG0780041359.01 | 0.806760 | 7.033295e-50 | 3.849006e-47 |
| MsG0480021015.01 | MsG0780041546.01 | 0.866260 | 3.248259e-65 | 1.087918e-61 |
| MsG0480021015.01 | MsG0880045015.01 | 0.867768 | 1.072441e-65 | 3.788052e-62 |
| MsG0380017605.01 | MsG0480021015.01 | 0.805486 | 1.304495e-49 | 6.909945e-47 |
| MsG0280007919.01 | MsG0480021015.01 | 0.823060 | 1.697108e-53 | 1.445831e-50 |
| MsG0280010346.01 | MsG0480021015.01 | 0.807785 | 4.264926e-50 | 2.397044e-47 |
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021015.01.T01 | MTR_4g063220 | 98.649 | 148 | 2 | 0 | 1 | 148 | 1 | 148 | 2.12e-101 | 287 |
| MsG0480021015.01.T01 | MTR_4g063990 | 98.649 | 148 | 2 | 0 | 1 | 148 | 1 | 148 | 2.12e-101 | 287 |
| MsG0480021015.01.T01 | MTR_4g063200 | 98.649 | 148 | 2 | 0 | 1 | 148 | 1 | 148 | 2.12e-101 | 287 |
| MsG0480021015.01.T01 | MTR_4g064020 | 98.649 | 148 | 2 | 0 | 1 | 148 | 1 | 148 | 2.12e-101 | 287 |
| MsG0480021015.01.T01 | MTR_4g071180 | 98.649 | 148 | 2 | 0 | 1 | 148 | 1 | 148 | 3.84e-101 | 286 |
| MsG0480021015.01.T01 | MTR_4g070240 | 98.649 | 148 | 2 | 0 | 1 | 148 | 1 | 148 | 3.84e-101 | 286 |
| MsG0480021015.01.T01 | MTR_4g070245 | 97.297 | 148 | 4 | 0 | 1 | 148 | 1 | 148 | 1.17e-99 | 283 |
| MsG0480021015.01.T01 | MTR_4g063240 | 97.973 | 148 | 2 | 1 | 1 | 148 | 1 | 147 | 3.77e-99 | 281 |
| MsG0480021015.01.T01 | MTR_4g064983 | 97.917 | 144 | 3 | 0 | 1 | 144 | 1 | 144 | 3.65e-96 | 277 |
| MsG0480021015.01.T01 | MTR_4g088025 | 94.595 | 148 | 3 | 1 | 1 | 148 | 1 | 143 | 3.38e-95 | 271 |
| MsG0480021015.01.T01 | MTR_4g063275 | 95.946 | 148 | 6 | 0 | 1 | 148 | 1 | 148 | 6.96e-93 | 265 |
| MsG0480021015.01.T01 | MTR_1g090780 | 89.189 | 148 | 5 | 3 | 1 | 148 | 1 | 137 | 2.00e-78 | 228 |
| MsG0480021015.01.T01 | MTR_1g492910 | 88.514 | 148 | 6 | 3 | 1 | 148 | 1 | 137 | 9.01e-78 | 227 |
| MsG0480021015.01.T01 | MTR_1g068600 | 92.793 | 111 | 4 | 1 | 38 | 148 | 30 | 136 | 3.59e-64 | 192 |
| MsG0480021015.01.T01 | MTR_7g099960 | 92.793 | 111 | 5 | 1 | 38 | 148 | 31 | 138 | 7.31e-64 | 192 |
| MsG0480021015.01.T01 | MTR_2g084480 | 61.798 | 89 | 34 | 0 | 59 | 147 | 136 | 224 | 5.44e-37 | 126 |
| MsG0480021015.01.T01 | MTR_8g069990 | 67.742 | 93 | 22 | 3 | 15 | 107 | 4 | 88 | 1.48e-33 | 114 |
| MsG0480021015.01.T01 | MTR_4g063270 | 90.196 | 51 | 5 | 0 | 86 | 136 | 1 | 51 | 5.54e-27 | 95.9 |
| MsG0480021015.01.T01 | MTR_7g114580 | 90.244 | 41 | 4 | 0 | 108 | 148 | 11 | 51 | 9.26e-20 | 77.4 |
| MsG0480021015.01.T01 | MTR_4g032777 | 36.792 | 106 | 63 | 2 | 31 | 132 | 5 | 110 | 3.65e-13 | 62.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021015.01.T01 | AT3G45980 | 88.742 | 151 | 13 | 2 | 1 | 148 | 1 | 150 | 2.11e-83 | 241 |
| MsG0480021015.01.T01 | AT2G28720 | 86.093 | 151 | 18 | 1 | 1 | 148 | 1 | 151 | 1.35e-80 | 234 |
| MsG0480021015.01.T01 | AT1G07790 | 86.275 | 153 | 11 | 3 | 1 | 148 | 1 | 148 | 1.25e-79 | 232 |
| MsG0480021015.01.T01 | AT5G59910 | 91.176 | 136 | 9 | 2 | 13 | 148 | 18 | 150 | 2.63e-77 | 226 |
| MsG0480021015.01.T01 | AT3G46030 | 94.444 | 126 | 6 | 1 | 23 | 148 | 21 | 145 | 1.07e-75 | 222 |
| MsG0480021015.01.T01 | AT2G37470 | 81.757 | 148 | 16 | 3 | 1 | 147 | 1 | 138 | 7.73e-71 | 209 |
| MsG0480021015.01.T01 | AT3G53650 | 81.879 | 149 | 15 | 3 | 1 | 148 | 1 | 138 | 1.02e-69 | 207 |
| MsG0480021015.01.T01 | AT5G22880 | 86.897 | 145 | 17 | 2 | 4 | 148 | 3 | 145 | 6.03e-67 | 200 |
| MsG0480021015.01.T01 | AT5G02570 | 79.730 | 148 | 14 | 1 | 1 | 148 | 1 | 132 | 1.14e-66 | 199 |
| MsG0480021015.01.T01 | AT3G09480 | 84.127 | 126 | 16 | 1 | 23 | 148 | 5 | 126 | 2.62e-62 | 187 |
| MsG0480021015.01.T01 | AT1G08170 | 56.989 | 93 | 40 | 0 | 53 | 145 | 143 | 235 | 1.79e-37 | 128 |
Find 42 sgRNAs with CRISPR-Local
Find 40 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCCTTGGGAAGCTTCTTTC+CGG | 0.267514 | 4:-52118211 | None:intergenic |
| CTTCTCCGCCGGAGCTTTCT+CGG | 0.304143 | 4:-52118178 | None:intergenic |
| GGAAGCTTCTTTCCGGCCTT+TGG | 0.308968 | 4:-52118204 | None:intergenic |
| CTCCTCTGCGGGTTTCTTCT+CGG | 0.332683 | 4:-52118139 | None:intergenic |
| TTGCTTGAGATACCAATGTC+TGG | 0.335174 | 4:-52118330 | None:intergenic |
| GGCGGCGGAACCACCTTCCT+TGG | 0.336629 | 4:-52118226 | None:intergenic |
| GCTGGCTTCTTCTCTCCCTT+TGG | 0.360478 | 4:-52118117 | None:intergenic |
| ACTTGTTCTTCCCGGAGAAT+TGG | 0.363793 | 4:+52118479 | MsG0480021015.01.T01:CDS |
| GCTGTCAGACTTGTTCTTCC+CGG | 0.392312 | 4:+52118471 | MsG0480021015.01.T01:CDS |
| CTTCTTCTTGTCTCCGGCGG+CGG | 0.396932 | 4:-52118241 | None:intergenic |
| GTGGACTTCTTCTCCTCTGC+GGG | 0.426139 | 4:-52118150 | None:intergenic |
| ATTTCCCTGGAAGTGATTGT+TGG | 0.430207 | 4:-52118441 | None:intergenic |
| TCTGCGGGTTTCTTCTCGGC+TGG | 0.440883 | 4:-52118135 | None:intergenic |
| GCCTTTGGCTTCTTCTCCGC+CGG | 0.441924 | 4:-52118189 | None:intergenic |
| TCTCTTCTTCTTCTTGTCTC+CGG | 0.444808 | 4:-52118247 | None:intergenic |
| GCGGCGGAACCACCTTCCTT+GGG | 0.454706 | 4:-52118225 | None:intergenic |
| CGCCGGAGCTTTCTCGGCAA+CGG | 0.475944 | 4:-52118172 | None:intergenic |
| AGAAGCCAACAATCACTTCC+AGG | 0.480188 | 4:+52118436 | MsG0480021015.01.T01:CDS |
| AGCATGCTTAGCCAATTCTC+CGG | 0.485650 | 4:-52118490 | None:intergenic |
| AAGGAAGGTGGTTCCGCCGC+CGG | 0.489907 | 4:+52118228 | MsG0480021015.01.T01:CDS |
| GACAGCAGTCTGAATTTCCC+TGG | 0.496873 | 4:-52118454 | None:intergenic |
| CACCGTTGCCGAGAAAGCTC+CGG | 0.523576 | 4:+52118170 | MsG0480021015.01.T01:CDS |
| GCGGAGAAGAAGCCAAAGGC+CGG | 0.524477 | 4:+52118192 | MsG0480021015.01.T01:CDS |
| ATTCGATTCAATGGCACCAA+AGG | 0.528131 | 4:+52118101 | None:intergenic |
| GCTAAGCATGCTGTTTCTGA+AGG | 0.531487 | 4:+52118501 | MsG0480021015.01.T01:CDS |
| TGCTTGAGATACCAATGTCT+GGG | 0.532971 | 4:-52118329 | None:intergenic |
| TGGTATCTCAAGCAAAGCCA+TGG | 0.533917 | 4:+52118338 | MsG0480021015.01.T01:CDS |
| TTCGATTCAATGGCACCAAA+GGG | 0.543023 | 4:+52118102 | None:intergenic |
| GCATGCTTAGCCAATTCTCC+GGG | 0.551301 | 4:-52118489 | None:intergenic |
| GGAAAGAAGCTTCCCAAGGA+AGG | 0.608356 | 4:+52118213 | MsG0480021015.01.T01:CDS |
| AAACAAGTTCACCCAGACAT+TGG | 0.612279 | 4:+52118318 | MsG0480021015.01.T01:CDS |
| GGTGGACTTCTTCTCCTCTG+CGG | 0.628573 | 4:-52118151 | None:intergenic |
| AAGAAGCTTCCCAAGGAAGG+TGG | 0.629895 | 4:+52118216 | MsG0480021015.01.T01:CDS |
| GAAACTGTTCATGATTCCCA+TGG | 0.633384 | 4:-52118355 | None:intergenic |
| GAAGCCAACAATCACTTCCA+GGG | 0.639164 | 4:+52118437 | MsG0480021015.01.T01:CDS |
| GGCCGGAAAGAAGCTTCCCA+AGG | 0.642326 | 4:+52118209 | MsG0480021015.01.T01:CDS |
| CGTTGCCGAGAAAGCTCCGG+CGG | 0.643171 | 4:+52118173 | MsG0480021015.01.T01:CDS |
| TCCGGCGGAGAAGAAGCCAA+AGG | 0.649331 | 4:+52118188 | MsG0480021015.01.T01:CDS |
| CGGAGCTTTCTCGGCAACGG+TGG | 0.653690 | 4:-52118169 | None:intergenic |
| GGTATCTCAAGCAAAGCCAT+GGG | 0.654333 | 4:+52118339 | MsG0480021015.01.T01:CDS |
| CTTCTTCTTCTTGTCTCCGG+CGG | 0.655854 | 4:-52118244 | None:intergenic |
| AGCCGAGAAGAAACCCGCAG+AGG | 0.676003 | 4:+52118137 | MsG0480021015.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAACAAGTTCACCCAGACAT+TGG | + | Chr4:52118318-52118337 | MsG0480021015.01.T01:CDS | 40.0% | |
| ATTTCCCTGGAAGTGATTGT+TGG | - | Chr4:52118444-52118463 | None:intergenic | 40.0% | |
| GAAACTGTTCATGATTCCCA+TGG | - | Chr4:52118358-52118377 | None:intergenic | 40.0% | |
| TCTCTTCTTCTTCTTGTCTC+CGG | - | Chr4:52118250-52118269 | None:intergenic | 40.0% | |
| TGCTTGAGATACCAATGTCT+GGG | - | Chr4:52118332-52118351 | None:intergenic | 40.0% | |
| TTGCTTGAGATACCAATGTC+TGG | - | Chr4:52118333-52118352 | None:intergenic | 40.0% | |
| ACTTGTTCTTCCCGGAGAAT+TGG | + | Chr4:52118479-52118498 | MsG0480021015.01.T01:CDS | 45.0% | |
| AGAAGCCAACAATCACTTCC+AGG | + | Chr4:52118436-52118455 | MsG0480021015.01.T01:CDS | 45.0% | |
| AGCATGCTTAGCCAATTCTC+CGG | - | Chr4:52118493-52118512 | None:intergenic | 45.0% | |
| GAAGCCAACAATCACTTCCA+GGG | + | Chr4:52118437-52118456 | MsG0480021015.01.T01:CDS | 45.0% | |
| GGTATCTCAAGCAAAGCCAT+GGG | + | Chr4:52118339-52118358 | MsG0480021015.01.T01:CDS | 45.0% | |
| TGGTATCTCAAGCAAAGCCA+TGG | + | Chr4:52118338-52118357 | MsG0480021015.01.T01:CDS | 45.0% | |
| TTCCTTGGGAAGCTTCTTTC+CGG | - | Chr4:52118214-52118233 | None:intergenic | 45.0% | |
| !! | GCTAAGCATGCTGTTTCTGA+AGG | + | Chr4:52118501-52118520 | MsG0480021015.01.T01:CDS | 45.0% |
| AAGAAGCTTCCCAAGGAAGG+TGG | + | Chr4:52118216-52118235 | MsG0480021015.01.T01:CDS | 50.0% | |
| CTTCTTCTTCTTGTCTCCGG+CGG | - | Chr4:52118247-52118266 | None:intergenic | 50.0% | |
| GACAGCAGTCTGAATTTCCC+TGG | - | Chr4:52118457-52118476 | None:intergenic | 50.0% | |
| GCATGCTTAGCCAATTCTCC+GGG | - | Chr4:52118492-52118511 | None:intergenic | 50.0% | |
| GGAAAGAAGCTTCCCAAGGA+AGG | + | Chr4:52118213-52118232 | MsG0480021015.01.T01:CDS | 50.0% | |
| ! | GCTGTCAGACTTGTTCTTCC+CGG | + | Chr4:52118471-52118490 | MsG0480021015.01.T01:CDS | 50.0% |
| CTCCTCTGCGGGTTTCTTCT+CGG | - | Chr4:52118142-52118161 | None:intergenic | 55.0% | |
| GGAAGCTTCTTTCCGGCCTT+TGG | - | Chr4:52118207-52118226 | None:intergenic | 55.0% | |
| GTGGACTTCTTCTCCTCTGC+GGG | - | Chr4:52118153-52118172 | None:intergenic | 55.0% | |
| ! | GCTGGCTTCTTCTCTCCCTT+TGG | - | Chr4:52118120-52118139 | None:intergenic | 55.0% |
| ! | GGTGGACTTCTTCTCCTCTG+CGG | - | Chr4:52118154-52118173 | None:intergenic | 55.0% |
| AGCCGAGAAGAAACCCGCAG+AGG | + | Chr4:52118137-52118156 | MsG0480021015.01.T01:CDS | 60.0% | |
| CACCGTTGCCGAGAAAGCTC+CGG | + | Chr4:52118170-52118189 | MsG0480021015.01.T01:CDS | 60.0% | |
| CTTCTCCGCCGGAGCTTTCT+CGG | - | Chr4:52118181-52118200 | None:intergenic | 60.0% | |
| CTTCTTCTTGTCTCCGGCGG+CGG | - | Chr4:52118244-52118263 | None:intergenic | 60.0% | |
| GCGGAGAAGAAGCCAAAGGC+CGG | + | Chr4:52118192-52118211 | MsG0480021015.01.T01:CDS | 60.0% | |
| GGCCGGAAAGAAGCTTCCCA+AGG | + | Chr4:52118209-52118228 | MsG0480021015.01.T01:CDS | 60.0% | |
| TCCGGCGGAGAAGAAGCCAA+AGG | + | Chr4:52118188-52118207 | MsG0480021015.01.T01:CDS | 60.0% | |
| TCTGCGGGTTTCTTCTCGGC+TGG | - | Chr4:52118138-52118157 | None:intergenic | 60.0% | |
| ! | GCCTTTGGCTTCTTCTCCGC+CGG | - | Chr4:52118192-52118211 | None:intergenic | 60.0% |
| AAGGAAGGTGGTTCCGCCGC+CGG | + | Chr4:52118228-52118247 | MsG0480021015.01.T01:CDS | 65.0% | |
| CGCCGGAGCTTTCTCGGCAA+CGG | - | Chr4:52118175-52118194 | None:intergenic | 65.0% | |
| CGGAGCTTTCTCGGCAACGG+TGG | - | Chr4:52118172-52118191 | None:intergenic | 65.0% | |
| CGTTGCCGAGAAAGCTCCGG+CGG | + | Chr4:52118173-52118192 | MsG0480021015.01.T01:CDS | 65.0% | |
| GCGGCGGAACCACCTTCCTT+GGG | - | Chr4:52118228-52118247 | None:intergenic | 65.0% | |
| GGCGGCGGAACCACCTTCCT+TGG | - | Chr4:52118229-52118248 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 52118111 | 52118557 | 52118111 | ID=MsG0480021015.01;Name=MsG0480021015.01 |
| Chr4 | mRNA | 52118111 | 52118557 | 52118111 | ID=MsG0480021015.01.T01;Parent=MsG0480021015.01;Name=MsG0480021015.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|148 |
| Chr4 | exon | 52118111 | 52118557 | 52118111 | ID=MsG0480021015.01.T01:exon:739;Parent=MsG0480021015.01.T01 |
| Chr4 | CDS | 52118111 | 52118557 | 52118111 | ID=MsG0480021015.01.T01:cds;Parent=MsG0480021015.01.T01 |
| Gene Sequence |
| Protein sequence |
