AlfalfaGEDB | MsG0480020920.01

M. sativa cultivar ZhongmuNo.1 / MsG0480020920.01

KEGG: K11252
GO: GO:0000786 GO:0003677 GO:0046982
PFAM: PF00125

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480020920.01.T01	XP_003606627.1	98.649	148	2	0	1	148	1	148	1.75E-97	287

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480020920.01.T01	Q1S9I9	98.649	148	2	0	1	148	1	148	3.94E-100	286

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480020920.01.T01	G7JTQ7	98.649	148	2	0	1	148	1	148	8.37e-98	287

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048220.01	MsG0480020920.01	0.804337	2.267352e-49	1.166002e-46
MsG0180005048.01	MsG0480020920.01	0.825495	4.544419e-54	4.148806e-51
MsG0480020918.01	MsG0480020920.01	0.804790	1.823655e-49	9.489114e-47
MsG0480020919.01	MsG0480020920.01	0.832934	7.118598e-56	8.075213e-53
MsG0480020920.01	MsG0480020921.01	0.838549	2.692221e-57	3.618181e-54
MsG0480020920.01	MsG0480020926.01	0.835438	1.677903e-56	2.051812e-53
MsG0480020920.01	MsG0480020927.01	0.821006	5.078333e-53	4.082664e-50
MsG0480020920.01	MsG0480020969.01	0.816712	4.799877e-52	3.426700e-49
MsG0480020920.01	MsG0480020970.01	0.811119	8.209562e-51	5.039160e-48
MsG0480020920.01	MsG0480020971.01	0.854227	1.416699e-61	3.142462e-58
MsG0480020920.01	MsG0480021015.01	0.878297	3.146326e-69	1.633978e-65
MsG0480020920.01	MsG0480021048.01	0.810264	1.256696e-50	7.540514e-48
MsG0480020920.01	MsG0480021049.01	0.806357	8.554746e-50	4.633626e-47
MsG0480020920.01	MsG0480021301.01	0.845086	5.058087e-59	8.334792e-56
MsG0480020920.01	MsG0480021302.01	0.851167	1.058285e-60	2.123202e-57
MsG0480020920.01	MsG0480022307.01	0.824422	8.143450e-54	7.209268e-51
MsG0480020920.01	MsG0480023424.01	0.811540	6.652460e-51	4.129115e-48
MsG0480020920.01	MsG0480023426.01	0.818254	2.157464e-52	1.606682e-49
MsG0480020920.01	MsG0580028955.01	0.811448	6.964551e-51	4.312417e-48
MsG0480020920.01	MsG0780040620.01	0.802161	6.397739e-49	3.111917e-46
MsG0480020920.01	MsG0780041273.01	0.849317	3.493889e-60	6.598206e-57
MsG0480020920.01	MsG0780041546.01	0.835704	1.437254e-56	1.771715e-53
MsG0480020920.01	MsG0880045015.01	0.804031	2.625535e-49	1.339669e-46

PPI

Gene1	Gene2	Type
MsG0880044910.01	MsG0480020920.01	PPI
MsG0880044910.01	MsG0480020920.01	PPI
MsG0380012788.01	MsG0480020920.01	PPI
MsG0280007919.01	MsG0480020920.01	PPI
MsG0580025504.01	MsG0480020920.01	PPI
MsG0180000243.01	MsG0480020920.01	PPI
MsG0580029194.01	MsG0480020920.01	PPI
MsG0480023842.01	MsG0480020920.01	PPI
MsG0580025506.01	MsG0480020920.01	PPI
MsG0880042844.01	MsG0480020920.01	PPI
MsG0580029044.01	MsG0480020920.01	PPI
MsG0480022307.01	MsG0480020920.01	PPI
MsG0780041592.01	MsG0480020920.01	PPI
MsG0880047260.01	MsG0480020920.01	PPI
MsG0780038642.01	MsG0480020920.01	PPI
MsG0280008380.01	MsG0480020920.01	PPI
MsG0480020920.01	MsG0780036534.01	PPI
MsG0880045015.01	MsG0480020920.01	PPI
MsG0580027231.01	MsG0480020920.01	PPI
MsG0780041593.01	MsG0480020920.01	PPI
MsG0480020877.01	MsG0480020920.01	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480020920.01.T01	MTR_4g063220	98.649	148	2	0	1	148	1	148	2.12e-101	287
MsG0480020920.01.T01	MTR_4g063990	98.649	148	2	0	1	148	1	148	2.12e-101	287
MsG0480020920.01.T01	MTR_4g063200	98.649	148	2	0	1	148	1	148	2.12e-101	287
MsG0480020920.01.T01	MTR_4g064020	98.649	148	2	0	1	148	1	148	2.12e-101	287
MsG0480020920.01.T01	MTR_4g071180	98.649	148	2	0	1	148	1	148	3.84e-101	286
MsG0480020920.01.T01	MTR_4g070240	98.649	148	2	0	1	148	1	148	3.84e-101	286
MsG0480020920.01.T01	MTR_4g070245	97.297	148	4	0	1	148	1	148	1.17e-99	283
MsG0480020920.01.T01	MTR_4g063240	97.973	148	2	1	1	148	1	147	3.77e-99	281
MsG0480020920.01.T01	MTR_4g064983	97.917	144	3	0	1	144	1	144	3.65e-96	277
MsG0480020920.01.T01	MTR_4g088025	94.595	148	3	1	1	148	1	143	3.38e-95	271
MsG0480020920.01.T01	MTR_4g063275	95.946	148	6	0	1	148	1	148	6.96e-93	265
MsG0480020920.01.T01	MTR_1g090780	89.189	148	5	3	1	148	1	137	2.00e-78	228
MsG0480020920.01.T01	MTR_1g492910	88.514	148	6	3	1	148	1	137	9.01e-78	227
MsG0480020920.01.T01	MTR_1g068600	92.793	111	4	1	38	148	30	136	3.59e-64	192
MsG0480020920.01.T01	MTR_7g099960	92.793	111	5	1	38	148	31	138	7.31e-64	192
MsG0480020920.01.T01	MTR_2g084480	61.798	89	34	0	59	147	136	224	5.44e-37	126
MsG0480020920.01.T01	MTR_8g069990	67.742	93	22	3	15	107	4	88	1.48e-33	114
MsG0480020920.01.T01	MTR_4g063270	90.196	51	5	0	86	136	1	51	5.54e-27	95.9
MsG0480020920.01.T01	MTR_7g114580	90.244	41	4	0	108	148	11	51	9.26e-20	77.4
MsG0480020920.01.T01	MTR_4g032777	36.792	106	63	2	31	132	5	110	3.65e-13	62.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480020920.01.T01	AT3G45980	88.742	151	13	2	1	148	1	150	2.11e-83	241
MsG0480020920.01.T01	AT2G28720	86.093	151	18	1	1	148	1	151	1.35e-80	234
MsG0480020920.01.T01	AT1G07790	86.275	153	11	3	1	148	1	148	1.25e-79	232
MsG0480020920.01.T01	AT5G59910	91.176	136	9	2	13	148	18	150	2.63e-77	226
MsG0480020920.01.T01	AT3G46030	94.444	126	6	1	23	148	21	145	1.07e-75	222
MsG0480020920.01.T01	AT2G37470	81.757	148	16	3	1	147	1	138	7.73e-71	209
MsG0480020920.01.T01	AT3G53650	81.879	149	15	3	1	148	1	138	1.02e-69	207
MsG0480020920.01.T01	AT5G22880	86.897	145	17	2	4	148	3	145	6.03e-67	200
MsG0480020920.01.T01	AT5G02570	79.730	148	14	1	1	148	1	132	1.14e-66	199
MsG0480020920.01.T01	AT3G09480	84.127	126	16	1	23	148	5	126	2.62e-62	187
MsG0480020920.01.T01	AT1G08170	56.989	93	40	0	53	145	143	235	1.79e-37	128

Find 43 sgRNAs with CRISPR-Local

Find 40 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCAGTCAGGCTTGTTCTTCC+TGG	0.277388	4:-50600673	MsG0480020920.01.T01:CDS
GCTTGTTCTTCCTGGTGAAT+TGG	0.309450	4:-50600665	MsG0480020920.01.T01:CDS
CCCGGCCTTTGGCTTCTTCT+CGG	0.319891	4:+50600951	None:intergenic
TTGCTTGAGATACCAATGTC+TGG	0.335174	4:+50600814	None:intergenic
TTCCTTAGGCAGCTTCTTCC+CGG	0.340242	4:+50600933	None:intergenic
GGCAGCTTCTTCCCGGCCTT+TGG	0.340806	4:+50600940	None:intergenic
GCTGGCTTCTTCTCTCCCTT+TGG	0.368494	4:+50601027	None:intergenic
CTTGTTGTAGCGAGCAAGTC+TGG	0.379852	4:+50600729	None:intergenic
GCCTTTGGCTTCTTCTCGGC+CGG	0.416799	4:+50600955	None:intergenic
TCCTTCAGAAACAGCATGCT+TGG	0.424952	4:+50600642	None:intergenic
GTGGATTTCTTCTCCTCTGC+GGG	0.433451	4:+50600994	None:intergenic
GCCGAGAAGAAGCCAAAGGC+CGG	0.450246	4:-50600952	MsG0480020920.01.T01:CDS
AGTCTAAGAACTTGTAAACT+TGG	0.460073	4:+50600606	None:intergenic
CTGAATTTCCCTTGAAGTGA+TGG	0.460281	4:+50600699	None:intergenic
TCTGCGGGTTTCTTCTCCGC+TGG	0.466008	4:+50601009	None:intergenic
TCTCTTCTTCTTCTTGTCTC+CGG	0.474508	4:+50600897	None:intergenic
AAGGAAGGTGGTTCAGCTGC+CGG	0.489603	4:-50600916	MsG0480020920.01.T01:CDS
GCAGCTGAACCACCTTCCTT+AGG	0.492686	4:+50600919	None:intergenic
CCGAGAAGAAGCCAAAGGCC+GGG	0.504134	4:-50600951	MsG0480020920.01.T01:CDS
GGGAAATTCAGACTGCAGTC+AGG	0.504618	4:-50600687	MsG0480020920.01.T01:CDS
ATTTCCCTTGAAGTGATGGT+TGG	0.507260	4:+50600703	None:intergenic
GCCAAGCATGCTGTTTCTGA+AGG	0.521533	4:-50600643	MsG0480020920.01.T01:CDS
GCATGCTTGGCCAATTCACC+AGG	0.526902	4:+50600655	None:intergenic
TGCTTGAGATACCAATGTCT+GGG	0.532971	4:+50600815	None:intergenic
TGGTATCTCAAGCAAAGCCA+TGG	0.533917	4:-50600806	MsG0480020920.01.T01:CDS
GAAATCCACCGTCGCAGAGA+AGG	0.535764	4:-50600980	MsG0480020920.01.T01:CDS
GAAATTCTCAATGGCACCAA+AGG	0.540291	4:-50601043	None:intergenic
GGCCGGAGCCTTCTCTGCGA+CGG	0.547872	4:+50600972	None:intergenic
AGAAGCCAACCATCACTTCA+AGG	0.552455	4:-50600708	MsG0480020920.01.T01:CDS
CGGAGCCTTCTCTGCGACGG+TGG	0.553608	4:+50600975	None:intergenic
AAATTCTCAATGGCACCAAA+GGG	0.562232	4:-50601042	None:intergenic
CACCGTCGCAGAGAAGGCTC+CGG	0.576144	4:-50600974	MsG0480020920.01.T01:CDS
GGCCGGGAAGAAGCTGCCTA+AGG	0.588407	4:-50600935	MsG0480020920.01.T01:CDS
GGGAAGAAGCTGCCTAAGGA+AGG	0.592588	4:-50600931	MsG0480020920.01.T01:CDS
AAACAAGTTCACCCAGACAT+TGG	0.612279	4:-50600826	MsG0480020920.01.T01:CDS
TCCGGCCGAGAAGAAGCCAA+AGG	0.621006	4:-50600956	MsG0480020920.01.T01:CDS
GAAGCCAACCATCACTTCAA+GGG	0.622283	4:-50600707	MsG0480020920.01.T01:CDS
GAAACTGTTCATGATTCCCA+TGG	0.633384	4:+50600789	None:intergenic
AAGAAGCTGCCTAAGGAAGG+TGG	0.636978	4:-50600928	MsG0480020920.01.T01:CDS
AAAGGGAGAGAAGAAGCCAG+CGG	0.642633	4:-50601025	MsG0480020920.01.T01:CDS
GGTGGATTTCTTCTCCTCTG+CGG	0.645875	4:+50600993	None:intergenic
GGTATCTCAAGCAAAGCCAT+GGG	0.654333	4:-50600805	MsG0480020920.01.T01:CDS
AGCGGAGAAGAAACCCGCAG+AGG	0.676003	4:-50601007	MsG0480020920.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AAACAAGTTCACCCAGACAT+TGG	-	Chr4:50600816-50600835	MsG0480020920.01.T01:CDS	40.0%
	ATTTCCCTTGAAGTGATGGT+TGG	+	Chr4:50600942-50600961	None:intergenic	40.0%
	CTGAATTTCCCTTGAAGTGA+TGG	+	Chr4:50600946-50600965	None:intergenic	40.0%
	GAAACTGTTCATGATTCCCA+TGG	+	Chr4:50600856-50600875	None:intergenic	40.0%
	TCTCTTCTTCTTCTTGTCTC+CGG	+	Chr4:50600748-50600767	None:intergenic	40.0%
	TGCTTGAGATACCAATGTCT+GGG	+	Chr4:50600830-50600849	None:intergenic	40.0%
	TTGCTTGAGATACCAATGTC+TGG	+	Chr4:50600831-50600850	None:intergenic	40.0%
	AGAAGCCAACCATCACTTCA+AGG	-	Chr4:50600934-50600953	MsG0480020920.01.T01:CDS	45.0%
	GAAGCCAACCATCACTTCAA+GGG	-	Chr4:50600935-50600954	MsG0480020920.01.T01:CDS	45.0%
	GCTTGTTCTTCCTGGTGAAT+TGG	-	Chr4:50600977-50600996	MsG0480020920.01.T01:CDS	45.0%
	GGTATCTCAAGCAAAGCCAT+GGG	-	Chr4:50600837-50600856	MsG0480020920.01.T01:CDS	45.0%
	TCCTTCAGAAACAGCATGCT+TGG	+	Chr4:50601003-50601022	None:intergenic	45.0%
	TGGTATCTCAAGCAAAGCCA+TGG	-	Chr4:50600836-50600855	MsG0480020920.01.T01:CDS	45.0%
	AAAGGGAGAGAAGAAGCCAG+CGG	-	Chr4:50600617-50600636	MsG0480020920.01.T01:CDS	50.0%
	AAGAAGCTGCCTAAGGAAGG+TGG	-	Chr4:50600714-50600733	MsG0480020920.01.T01:CDS	50.0%
	GGGAAATTCAGACTGCAGTC+AGG	-	Chr4:50600955-50600974	MsG0480020920.01.T01:CDS	50.0%
	GTGGATTTCTTCTCCTCTGC+GGG	+	Chr4:50600651-50600670	None:intergenic	50.0%
	TTCCTTAGGCAGCTTCTTCC+CGG	+	Chr4:50600712-50600731	None:intergenic	50.0%
!	CTTGTTGTAGCGAGCAAGTC+TGG	+	Chr4:50600916-50600935	None:intergenic	50.0%
!	GGTGGATTTCTTCTCCTCTG+CGG	+	Chr4:50600652-50600671	None:intergenic	50.0%
!!	GCCAAGCATGCTGTTTCTGA+AGG	-	Chr4:50600999-50601018	MsG0480020920.01.T01:CDS	50.0%
	AAGGAAGGTGGTTCAGCTGC+CGG	-	Chr4:50600726-50600745	MsG0480020920.01.T01:CDS	55.0%
	GAAATCCACCGTCGCAGAGA+AGG	-	Chr4:50600662-50600681	MsG0480020920.01.T01:CDS	55.0%
	GCAGCTGAACCACCTTCCTT+AGG	+	Chr4:50600726-50600745	None:intergenic	55.0%
	GCATGCTTGGCCAATTCACC+AGG	+	Chr4:50600990-50601009	None:intergenic	55.0%
	GGGAAGAAGCTGCCTAAGGA+AGG	-	Chr4:50600711-50600730	MsG0480020920.01.T01:CDS	55.0%
!	GCAGTCAGGCTTGTTCTTCC+TGG	-	Chr4:50600969-50600988	MsG0480020920.01.T01:CDS	55.0%
!	GCTGGCTTCTTCTCTCCCTT+TGG	+	Chr4:50600618-50600637	None:intergenic	55.0%
	AGCGGAGAAGAAACCCGCAG+AGG	-	Chr4:50600635-50600654	MsG0480020920.01.T01:CDS	60.0%
	CCGAGAAGAAGCCAAAGGCC+GGG	-	Chr4:50600691-50600710	MsG0480020920.01.T01:CDS	60.0%
	GCCGAGAAGAAGCCAAAGGC+CGG	-	Chr4:50600690-50600709	MsG0480020920.01.T01:CDS	60.0%
	TCCGGCCGAGAAGAAGCCAA+AGG	-	Chr4:50600686-50600705	MsG0480020920.01.T01:CDS	60.0%
	TCTGCGGGTTTCTTCTCCGC+TGG	+	Chr4:50600636-50600655	None:intergenic	60.0%
!	CCCGGCCTTTGGCTTCTTCT+CGG	+	Chr4:50600694-50600713	None:intergenic	60.0%
!	GCCTTTGGCTTCTTCTCGGC+CGG	+	Chr4:50600690-50600709	None:intergenic	60.0%
	CACCGTCGCAGAGAAGGCTC+CGG	-	Chr4:50600668-50600687	MsG0480020920.01.T01:CDS	65.0%
	GGCAGCTTCTTCCCGGCCTT+TGG	+	Chr4:50600705-50600724	None:intergenic	65.0%
	GGCCGGGAAGAAGCTGCCTA+AGG	-	Chr4:50600707-50600726	MsG0480020920.01.T01:CDS	65.0%
	CGGAGCCTTCTCTGCGACGG+TGG	+	Chr4:50600670-50600689	None:intergenic	70.0%
	GGCCGGAGCCTTCTCTGCGA+CGG	+	Chr4:50600673-50600692	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	50600609	50601055	50600609	ID=MsG0480020920.01;Name=MsG0480020920.01
Chr4	mRNA	50600609	50601055	50600609	ID=MsG0480020920.01.T01;Parent=MsG0480020920.01;Name=MsG0480020920.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|148
Chr4	exon	50600609	50601055	50600609	ID=MsG0480020920.01.T01:exon:325;Parent=MsG0480020920.01.T01
Chr4	CDS	50600609	50601055	50600609	ID=MsG0480020920.01.T01:cds;Parent=MsG0480020920.01.T01

Gene Sequence

>MsG0480020920.01.T01

ATGGCACCAAAGGGAGAGAAGAAGCCAGCGGAGAAGAAACCCGCAGAGGAGAAGAAATCCACCGTCGCAGAGAAGGCTCCGGCCGAGAAGAAGCCAAAGGCCGGGAAGAAGCTGCCTAAGGAAGGTGGTTCAGCTGCCGGAGACAAGAAGAAGAAGAGAAACAAGAAGAGCGTTGAAACATACAAGATATACATCTTCAAAGTTCTGAAACAAGTTCACCCAGACATTGGTATCTCAAGCAAAGCCATGGGAATCATGAACAGTTTCATCAATGATATCTTCGAGAAGCTTGCTCAAGAATCTTCCAGACTTGCTCGCTACAACAAGAAGCCAACCATCACTTCAAGGGAAATTCAGACTGCAGTCAGGCTTGTTCTTCCTGGTGAATTGGCCAAGCATGCTGTTTCTGAAGGAACAAAAGCTGTGACCAAGTTTACAAGTTCTTAG

Protein sequence

>MsG0480020920.01.T01

MAPKGEKKPAEKKPAEEKKSTVAEKAPAEKKPKAGKKLPKEGGSAAGDKKKKRNKKSVETYKIYIFKVLKQVHPDIGISSKAMGIMNSFINDIFEKLAQESSRLARYNKKPTITSREIQTAVRLVLPGELAKHAVSEGTKAVTKFTSS*