AlfalfaGEDB | MsG0480020969.01

M. sativa cultivar ZhongmuNo.1 / MsG0480020969.01

KEGG: K11252
GO: GO:0000786 GO:0003677 GO:0046982
PFAM: PF00125

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480020969.01.T01	XP_003606627.1	98.649	148	2	0	1	148	1	148	1.75E-97	287

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480020969.01.T01	Q1S9I9	98.649	148	2	0	1	148	1	148	3.94E-100	286

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480020969.01.T01	G7JTQ7	98.649	148	2	0	1	148	1	148	8.37e-98	287

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180004995.01	MsG0480020969.01	0.814391	1.577756e-51	1.057391e-48
MsG0180005048.01	MsG0480020969.01	0.829850	4.086544e-55	4.233783e-52
MsG0380014666.01	MsG0480020969.01	0.804428	2.170818e-49	1.118936e-46
MsG0480020919.01	MsG0480020969.01	0.804792	1.822551e-49	9.483673e-47
MsG0480020920.01	MsG0480020969.01	0.816712	4.799877e-52	3.426700e-49
MsG0480020921.01	MsG0480020969.01	0.802954	4.389871e-49	2.178781e-46
MsG0480020926.01	MsG0480020969.01	0.847222	1.325934e-59	2.340373e-56
MsG0480020927.01	MsG0480020969.01	0.830995	2.145222e-55	2.297550e-52
MsG0480020969.01	MsG0480020970.01	0.843719	1.178635e-58	1.860374e-55
MsG0480020969.01	MsG0480020971.01	0.874193	8.177790e-68	3.647430e-64
MsG0480020969.01	MsG0480021015.01	0.864831	9.158846e-65	2.915530e-61
MsG0480020969.01	MsG0480021048.01	0.803046	4.201903e-49	2.090345e-46
MsG0480020969.01	MsG0480021301.01	0.850807	1.337027e-60	2.651045e-57
MsG0480020969.01	MsG0480021302.01	0.866982	1.913336e-65	6.573238e-62
MsG0480020969.01	MsG0780036534.01	0.805483	1.305998e-49	6.917487e-47
MsG0480020969.01	MsG0780041273.01	0.827949	1.179020e-54	1.155334e-51
MsG0480020969.01	MsG0780041546.01	0.847019	1.506959e-59	2.642517e-56
MsG0480020969.01	MsG0880045015.01	0.802272	6.070850e-49	2.961240e-46
MsG0280010346.01	MsG0480020969.01	0.809257	2.069625e-50	1.209275e-47

PPI

Gene1	Gene2	Type
MsG0480020969.01	MsG0780038642.01	PPI
MsG0480020969.01	MsG0380012788.01	PPI
MsG0480020969.01	MsG0280008380.01	PPI
MsG0480020969.01	MsG0180000243.01	PPI
MsG0480020969.01	MsG0780041592.01	PPI
MsG0480020969.01	MsG0480022307.01	PPI
MsG0480020969.01	MsG0580029044.01	PPI
MsG0480020969.01	MsG0880042844.01	PPI
MsG0480020969.01	MsG0780041593.01	PPI
MsG0480020969.01	MsG0880044910.01	PPI
MsG0480020969.01	MsG0880047260.01	PPI
MsG0480020969.01	MsG0780036534.01	PPI
MsG0580025506.01	MsG0480020969.01	PPI
MsG0480020969.01	MsG0880045015.01	PPI
MsG0480020969.01	MsG0280007919.01	PPI
MsG0480020969.01	MsG0480023842.01	PPI
MsG0480020969.01	MsG0580027231.01	PPI
MsG0480020969.01	MsG0480020877.01	PPI
MsG0880044910.01	MsG0480020969.01	PPI
MsG0480020969.01	MsG0580029194.01	PPI
MsG0480020969.01	MsG0580025504.01	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480020969.01.T01	MTR_4g063220	98.649	148	2	0	1	148	1	148	2.12e-101	287
MsG0480020969.01.T01	MTR_4g063990	98.649	148	2	0	1	148	1	148	2.12e-101	287
MsG0480020969.01.T01	MTR_4g063200	98.649	148	2	0	1	148	1	148	2.12e-101	287
MsG0480020969.01.T01	MTR_4g064020	98.649	148	2	0	1	148	1	148	2.12e-101	287
MsG0480020969.01.T01	MTR_4g071180	98.649	148	2	0	1	148	1	148	3.84e-101	286
MsG0480020969.01.T01	MTR_4g070240	98.649	148	2	0	1	148	1	148	3.84e-101	286
MsG0480020969.01.T01	MTR_4g070245	97.297	148	4	0	1	148	1	148	1.17e-99	283
MsG0480020969.01.T01	MTR_4g063240	97.973	148	2	1	1	148	1	147	3.77e-99	281
MsG0480020969.01.T01	MTR_4g064983	97.917	144	3	0	1	144	1	144	3.65e-96	277
MsG0480020969.01.T01	MTR_4g088025	94.595	148	3	1	1	148	1	143	3.38e-95	271
MsG0480020969.01.T01	MTR_4g063275	95.946	148	6	0	1	148	1	148	6.96e-93	265
MsG0480020969.01.T01	MTR_1g090780	89.189	148	5	3	1	148	1	137	2.00e-78	228
MsG0480020969.01.T01	MTR_1g492910	88.514	148	6	3	1	148	1	137	9.01e-78	227
MsG0480020969.01.T01	MTR_1g068600	92.793	111	4	1	38	148	30	136	3.59e-64	192
MsG0480020969.01.T01	MTR_7g099960	92.793	111	5	1	38	148	31	138	7.31e-64	192
MsG0480020969.01.T01	MTR_2g084480	61.798	89	34	0	59	147	136	224	5.44e-37	126
MsG0480020969.01.T01	MTR_8g069990	67.742	93	22	3	15	107	4	88	1.48e-33	114
MsG0480020969.01.T01	MTR_4g063270	90.196	51	5	0	86	136	1	51	5.54e-27	95.9
MsG0480020969.01.T01	MTR_7g114580	90.244	41	4	0	108	148	11	51	9.26e-20	77.4
MsG0480020969.01.T01	MTR_4g032777	36.792	106	63	2	31	132	5	110	3.65e-13	62.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480020969.01.T01	AT3G45980	88.742	151	13	2	1	148	1	150	2.11e-83	241
MsG0480020969.01.T01	AT2G28720	86.093	151	18	1	1	148	1	151	1.35e-80	234
MsG0480020969.01.T01	AT1G07790	86.275	153	11	3	1	148	1	148	1.25e-79	232
MsG0480020969.01.T01	AT5G59910	91.176	136	9	2	13	148	18	150	2.63e-77	226
MsG0480020969.01.T01	AT3G46030	94.444	126	6	1	23	148	21	145	1.07e-75	222
MsG0480020969.01.T01	AT2G37470	81.757	148	16	3	1	147	1	138	7.73e-71	209
MsG0480020969.01.T01	AT3G53650	81.879	149	15	3	1	148	1	138	1.02e-69	207
MsG0480020969.01.T01	AT5G22880	86.897	145	17	2	4	148	3	145	6.03e-67	200
MsG0480020969.01.T01	AT5G02570	79.730	148	14	1	1	148	1	132	1.14e-66	199
MsG0480020969.01.T01	AT3G09480	84.127	126	16	1	23	148	5	126	2.62e-62	187
MsG0480020969.01.T01	AT1G08170	56.989	93	40	0	53	145	143	235	1.79e-37	128

Find 40 sgRNAs with CRISPR-Local

Find 38 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTCCTTGGGAAGCTTCTTTC+CGG	0.267514	4:-51332135	None:intergenic
GCTGTCAGACTTGTTCTTCC+TGG	0.307278	4:+51332395	MsG0480020969.01.T01:CDS
ACTTGTTCTTCCTGGAGAAT+TGG	0.329733	4:+51332403	MsG0480020969.01.T01:CDS
GCTGGCTTCTTCTCTCCCTT+TGG	0.330331	4:-51332041	None:intergenic
TTGCTTGAGATACCAATGTC+TGG	0.335174	4:-51332254	None:intergenic
TCTGCGGGTTTCTTCTCTGC+TGG	0.399254	4:-51332059	None:intergenic
GGAAGCTTCTTTCCGGCCTT+CGG	0.420168	4:-51332128	None:intergenic
GGCAGCAGAACCTCCTTCCT+TGG	0.424531	4:-51332150	None:intergenic
ATTTCCCTGGAAGTGATTGT+TGG	0.430207	4:-51332365	None:intergenic
GTGGATTTCTTCTCCTCTGC+GGG	0.433451	4:-51332074	None:intergenic
TGTGAACTTAGTCACAGCTT+TGG	0.448279	4:-51332450	None:intergenic
TCTCTTCTTCTTCTTGTCTC+CGG	0.474508	4:-51332171	None:intergenic
ACCCTCAGAGACAGCATGCT+TGG	0.475334	4:-51332426	None:intergenic
AGAAGCCAACAATCACTTCC+AGG	0.480188	4:+51332360	MsG0480020969.01.T01:CDS
GCATGCTTGGCCAATTCTCC+AGG	0.487310	4:-51332413	None:intergenic
GCAGCAGAACCTCCTTCCTT+GGG	0.491480	4:-51332149	None:intergenic
GACAGCAGTCTGAATTTCCC+TGG	0.496873	4:-51332378	None:intergenic
GCGGAGAAGAAGCCGAAGGC+CGG	0.497699	4:+51332116	MsG0480020969.01.T01:CDS
AAGGAAGGAGGTTCTGCTGC+CGG	0.500031	4:+51332152	MsG0480020969.01.T01:CDS
GCCTTCGGCTTCTTCTCCGC+CGG	0.500185	4:-51332113	None:intergenic
CGCCGGAGCTTTCTCAGCGA+CGG	0.521178	4:-51332096	None:intergenic
TGCTTGAGATACCAATGTCT+GGG	0.532971	4:-51332253	None:intergenic
TGGTATCTCAAGCAAAGCCA+TGG	0.533917	4:+51332262	MsG0480020969.01.T01:CDS
ATTGGATTCAATGGCACCAA+AGG	0.536978	4:+51332025	None:intergenic
CACCGTCGCTGAGAAAGCTC+CGG	0.552253	4:+51332094	MsG0480020969.01.T01:CDS
GCCAAGCATGCTGTCTCTGA+GGG	0.566391	4:+51332425	MsG0480020969.01.T01:CDS
TTGGATTCAATGGCACCAAA+GGG	0.571348	4:+51332026	None:intergenic
GGCCAAGCATGCTGTCTCTG+AGG	0.598295	4:+51332424	MsG0480020969.01.T01:CDS
GGAAAGAAGCTTCCCAAGGA+AGG	0.599477	4:+51332137	MsG0480020969.01.T01:CDS
AAACAAGTTCACCCAGACAT+TGG	0.612279	4:+51332242	MsG0480020969.01.T01:CDS
GAAACTGTTCATGATTCCCA+TGG	0.633384	4:-51332279	None:intergenic
AAGAAGCTTCCCAAGGAAGG+AGG	0.634382	4:+51332140	MsG0480020969.01.T01:CDS
TCCGGCGGAGAAGAAGCCGA+AGG	0.636700	4:+51332112	MsG0480020969.01.T01:CDS
GAAGCCAACAATCACTTCCA+GGG	0.639164	4:+51332361	MsG0480020969.01.T01:CDS
CGTCGCTGAGAAAGCTCCGG+CGG	0.640688	4:+51332097	MsG0480020969.01.T01:CDS
GGCCGGAAAGAAGCTTCCCA+AGG	0.642326	4:+51332133	MsG0480020969.01.T01:CDS
GGTGGATTTCTTCTCCTCTG+CGG	0.645875	4:-51332075	None:intergenic
GGTATCTCAAGCAAAGCCAT+GGG	0.654333	4:+51332263	MsG0480020969.01.T01:CDS
CGGAGCTTTCTCAGCGACGG+TGG	0.663279	4:-51332093	None:intergenic
AGCAGAGAAGAAACCCGCAG+AGG	0.698236	4:+51332061	MsG0480020969.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AAACAAGTTCACCCAGACAT+TGG	+	Chr4:51332242-51332261	MsG0480020969.01.T01:CDS	40.0%
	ACTTGTTCTTCCTGGAGAAT+TGG	+	Chr4:51332403-51332422	MsG0480020969.01.T01:CDS	40.0%
	ATTTCCCTGGAAGTGATTGT+TGG	-	Chr4:51332368-51332387	None:intergenic	40.0%
	GAAACTGTTCATGATTCCCA+TGG	-	Chr4:51332282-51332301	None:intergenic	40.0%
	TCTCTTCTTCTTCTTGTCTC+CGG	-	Chr4:51332174-51332193	None:intergenic	40.0%
	TGCTTGAGATACCAATGTCT+GGG	-	Chr4:51332256-51332275	None:intergenic	40.0%
	TGTGAACTTAGTCACAGCTT+TGG	-	Chr4:51332453-51332472	None:intergenic	40.0%
	TTGCTTGAGATACCAATGTC+TGG	-	Chr4:51332257-51332276	None:intergenic	40.0%
	AGAAGCCAACAATCACTTCC+AGG	+	Chr4:51332360-51332379	MsG0480020969.01.T01:CDS	45.0%
	GAAGCCAACAATCACTTCCA+GGG	+	Chr4:51332361-51332380	MsG0480020969.01.T01:CDS	45.0%
	GGTATCTCAAGCAAAGCCAT+GGG	+	Chr4:51332263-51332282	MsG0480020969.01.T01:CDS	45.0%
	TGGTATCTCAAGCAAAGCCA+TGG	+	Chr4:51332262-51332281	MsG0480020969.01.T01:CDS	45.0%
	TTCCTTGGGAAGCTTCTTTC+CGG	-	Chr4:51332138-51332157	None:intergenic	45.0%
	AAGAAGCTTCCCAAGGAAGG+AGG	+	Chr4:51332140-51332159	MsG0480020969.01.T01:CDS	50.0%
	GACAGCAGTCTGAATTTCCC+TGG	-	Chr4:51332381-51332400	None:intergenic	50.0%
	GGAAAGAAGCTTCCCAAGGA+AGG	+	Chr4:51332137-51332156	MsG0480020969.01.T01:CDS	50.0%
	GTGGATTTCTTCTCCTCTGC+GGG	-	Chr4:51332077-51332096	None:intergenic	50.0%
!	GCTGTCAGACTTGTTCTTCC+TGG	+	Chr4:51332395-51332414	MsG0480020969.01.T01:CDS	50.0%
!	GGTGGATTTCTTCTCCTCTG+CGG	-	Chr4:51332078-51332097	None:intergenic	50.0%
	AAGGAAGGAGGTTCTGCTGC+CGG	+	Chr4:51332152-51332171	MsG0480020969.01.T01:CDS	55.0%
	ACCCTCAGAGACAGCATGCT+TGG	-	Chr4:51332429-51332448	None:intergenic	55.0%
	AGCAGAGAAGAAACCCGCAG+AGG	+	Chr4:51332061-51332080	MsG0480020969.01.T01:CDS	55.0%
	GCAGCAGAACCTCCTTCCTT+GGG	-	Chr4:51332152-51332171	None:intergenic	55.0%
	GCATGCTTGGCCAATTCTCC+AGG	-	Chr4:51332416-51332435	None:intergenic	55.0%
	GCCAAGCATGCTGTCTCTGA+GGG	+	Chr4:51332425-51332444	MsG0480020969.01.T01:CDS	55.0%
	GGAAGCTTCTTTCCGGCCTT+CGG	-	Chr4:51332131-51332150	None:intergenic	55.0%
	TCTGCGGGTTTCTTCTCTGC+TGG	-	Chr4:51332062-51332081	None:intergenic	55.0%
!	GCTGGCTTCTTCTCTCCCTT+TGG	-	Chr4:51332044-51332063	None:intergenic	55.0%
	CACCGTCGCTGAGAAAGCTC+CGG	+	Chr4:51332094-51332113	MsG0480020969.01.T01:CDS	60.0%
	GGCAGCAGAACCTCCTTCCT+TGG	-	Chr4:51332153-51332172	None:intergenic	60.0%
	GGCCAAGCATGCTGTCTCTG+AGG	+	Chr4:51332424-51332443	MsG0480020969.01.T01:CDS	60.0%
	GGCCGGAAAGAAGCTTCCCA+AGG	+	Chr4:51332133-51332152	MsG0480020969.01.T01:CDS	60.0%
	CGCCGGAGCTTTCTCAGCGA+CGG	-	Chr4:51332099-51332118	None:intergenic	65.0%
	CGGAGCTTTCTCAGCGACGG+TGG	-	Chr4:51332096-51332115	None:intergenic	65.0%
	CGTCGCTGAGAAAGCTCCGG+CGG	+	Chr4:51332097-51332116	MsG0480020969.01.T01:CDS	65.0%
	GCCTTCGGCTTCTTCTCCGC+CGG	-	Chr4:51332116-51332135	None:intergenic	65.0%
	GCGGAGAAGAAGCCGAAGGC+CGG	+	Chr4:51332116-51332135	MsG0480020969.01.T01:CDS	65.0%
	TCCGGCGGAGAAGAAGCCGA+AGG	+	Chr4:51332112-51332131	MsG0480020969.01.T01:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	51332035	51332481	51332035	ID=MsG0480020969.01;Name=MsG0480020969.01
Chr4	mRNA	51332035	51332481	51332035	ID=MsG0480020969.01.T01;Parent=MsG0480020969.01;Name=MsG0480020969.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|148
Chr4	exon	51332035	51332481	51332035	ID=MsG0480020969.01.T01:exon:1084;Parent=MsG0480020969.01.T01
Chr4	CDS	51332035	51332481	51332035	ID=MsG0480020969.01.T01:cds;Parent=MsG0480020969.01.T01

Gene Sequence

>MsG0480020969.01.T01

ATGGCACCAAAGGGAGAGAAGAAGCCAGCAGAGAAGAAACCCGCAGAGGAGAAGAAATCCACCGTCGCTGAGAAAGCTCCGGCGGAGAAGAAGCCGAAGGCCGGAAAGAAGCTTCCCAAGGAAGGAGGTTCTGCTGCCGGAGACAAGAAGAAGAAGAGAAACAAGAAGAGTGTAGAAACATACAAGATCTACATATTCAAAGTTCTGAAACAAGTTCACCCAGACATTGGTATCTCAAGCAAAGCCATGGGAATCATGAACAGTTTCATCAATGACATCTTCGAGAAGCTTGCTCAAGAATCATCAAGACTCGCTCGTTACAACAAGAAGCCAACAATCACTTCCAGGGAAATTCAGACTGCTGTCAGACTTGTTCTTCCTGGAGAATTGGCCAAGCATGCTGTCTCTGAGGGTACCAAAGCTGTGACTAAGTTCACAAGTTCTTAG

Protein sequence

>MsG0480020969.01.T01

MAPKGEKKPAEKKPAEEKKSTVAEKAPAEKKPKAGKKLPKEGGSAAGDKKKKRNKKSVETYKIYIFKVLKQVHPDIGISSKAMGIMNSFINDIFEKLAQESSRLARYNKKPTITSREIQTAVRLVLPGELAKHAVSEGTKAVTKFTSS*