AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180000243.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180000243.01.T01 MTR_1g019770 97.514 362 8 1 1 362 1 361 0.0 731
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180000243.01.T01 AT2G37560 66.762 349 110 2 20 362 14 362 1.99e-173 487
MsG0180000243.01.T01 AT2G37560 64.244 344 109 2 20 362 14 344 2.44e-170 478

Find 98 sgRNAs with CRISPR-Local

Find 214 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTAAACATGAAAAGGAAAT+TGG 0.153499 1:+3309180 MsG0180000243.01.T01:CDS
AACATGCCTTCAATCTTATA+TGG 0.207992 1:-3311775 None:intergenic
AAAGCTAAACGCAAGGTTTC+CGG 0.211000 1:+3310655 MsG0180000243.01.T01:CDS
TATTCTGTAGTTGTCATTAA+TGG 0.229602 1:+3310407 MsG0180000243.01.T01:CDS
TGGAGGCAACAATACGAATA+TGG 0.257115 1:-3310846 None:intergenic
GGCAGTTTGGGACAAGAATA+TGG 0.289237 1:+3311705 MsG0180000243.01.T01:intron
ATCCGCAACCGCACCCTTGC+CGG 0.304476 1:+3308815 MsG0180000243.01.T01:five_prime_UTR
AAATCAACCCGATAATAAAA+TGG 0.304978 1:+3308757 None:intergenic
ATATTGATGGACCAGGATTA+AGG 0.310552 1:+3310779 MsG0180000243.01.T01:CDS
GATATCAGTAATCTTGTGTT+TGG 0.329500 1:-3309022 None:intergenic
ACTTTCCGAAATTGTATGAT+AGG 0.333167 1:+3312286 MsG0180000243.01.T01:three_prime_UTR
CTTAGGGAGAGAAGCCATTC+AGG 0.346202 1:-3309234 None:intergenic
ATGATTCCAATGAAATTGTA+TGG 0.352404 1:+3312237 MsG0180000243.01.T01:three_prime_UTR
ATTCTGTAGTTGTCATTAAT+GGG 0.355542 1:+3310408 MsG0180000243.01.T01:CDS
AGATGAACAACAACGACAAT+TGG 0.357101 1:+3308911 MsG0180000243.01.T01:exon
AAATCGAAAGAGCGGTGGTT+AGG 0.357251 1:-3308883 None:intergenic
CTCCGGCAAGGGTGCGGTTG+CGG 0.369001 1:-3308817 MsG0180000242.01.T01:intron
CGAAGCCTATCATACAATTT+CGG 0.374910 1:-3312291 None:intergenic
GTAGTAATTCACAATATTGA+TGG 0.374954 1:+3310766 MsG0180000243.01.T01:CDS
GCTCTTCTGATGTATGGCTT+TGG 0.385885 1:+3310335 MsG0180000243.01.T01:CDS
ACATGCCTTCAATCTTATAT+GGG 0.410899 1:-3311774 None:intergenic
TCAGACCATTAACTTAAAAC+AGG 0.411589 1:+3310436 MsG0180000243.01.T01:CDS
TTGAATACACTCTGGGCATT+GGG 0.413303 1:-3311874 None:intergenic
TACTCTGAATTCTCATTTGA+CGG 0.418075 1:+3312078 MsG0180000243.01.T01:CDS
CCAGACGAGCGAGGTATTGT+TGG 0.423306 1:-3310813 None:intergenic
AATCGAAAGAGCGGTGGTTA+GGG 0.424912 1:-3308882 None:intergenic
ATATTAGGTTCTGATAGCTT+TGG 0.426600 1:+3310609 MsG0180000243.01.T01:intron
TGAAGTGCTTCGGTTGTAAG+AGG 0.428767 1:-3312166 None:intergenic
GGATTCGCTCTTCTGATGTA+TGG 0.431543 1:+3310329 MsG0180000243.01.T01:CDS
TTTGAATACACTCTGGGCAT+TGG 0.433936 1:-3311875 None:intergenic
CTAAACATGAAAAGGAAATT+GGG 0.440776 1:+3309181 MsG0180000243.01.T01:CDS
GAGCGGTGGTTAGGGTTATG+AGG 0.443337 1:-3308874 None:intergenic
TGGCTCACACACAGTTCAAT+TGG 0.450005 1:+3311725 MsG0180000243.01.T01:CDS
TATTGATGGACCAGGATTAA+GGG 0.455380 1:+3310780 MsG0180000243.01.T01:CDS
CAGACGAGCGAGGTATTGTT+GGG 0.459713 1:-3310812 None:intergenic
TTTGCCCCATATAAGATTGA+AGG 0.461148 1:+3311769 MsG0180000243.01.T01:CDS
GTTTCAGAGTCCCTTAATCC+TGG 0.463196 1:-3310790 None:intergenic
CCAACAATACCTCGCTCGTC+TGG 0.463209 1:+3310813 MsG0180000243.01.T01:CDS
GATAGGCTTCGTATATTGAA+TGG 0.464349 1:+3312303 MsG0180000243.01.T01:three_prime_UTR
TATTCAACCTAAACATGAAA+AGG 0.467955 1:+3309173 MsG0180000243.01.T01:CDS
GATGAACAACAACGACAATT+GGG 0.477909 1:+3308912 MsG0180000243.01.T01:exon
TCTTACATTGTTGTATGAAG+TGG 0.478921 1:+3310714 MsG0180000243.01.T01:CDS
CGCTACTGACAACGATGCTC+CGG 0.485151 1:-3308834 None:intergenic
ATCTTATATGGGGCAAATGT+AGG 0.486995 1:-3311763 None:intergenic
CTTGTGTTTGGATTTCTTCA+CGG 0.487363 1:-3309010 None:intergenic
CAAATATTTAGTCTCCTGTC+AGG 0.489852 1:-3312350 None:intergenic
GTATGGCTTTGGATCTAAGA+AGG 0.495801 1:+3310346 MsG0180000243.01.T01:CDS
TGATTCCAATGAAATTGTAT+GGG 0.495883 1:+3312238 MsG0180000243.01.T01:three_prime_UTR
GAGTAACCTGGCTGCTAACA+AGG 0.508129 1:-3312060 None:intergenic
TTGACCATCGGAATGCCTCT+TGG 0.512065 1:-3312128 None:intergenic
GGAGGCAACAATACGAATAT+GGG 0.514014 1:-3310845 None:intergenic
ATCTGAAGCTCATTCCTGAC+AGG 0.515329 1:+3312336 MsG0180000243.01.T01:three_prime_UTR
ACGTTTCCTTGTTAGCAGCC+AGG 0.517589 1:+3312054 MsG0180000243.01.T01:CDS
CAAATTTCTCATCCCGATGA+AGG 0.522168 1:+3311913 MsG0180000243.01.T01:CDS
GGGAGCATTAACATGGTCAA+TGG 0.522317 1:-3310866 None:intergenic
CAACCCGATAATAAAATGGC+GGG 0.525311 1:+3308761 None:intergenic
CTGAATGGCTTCTCTCCCTA+AGG 0.525332 1:+3309235 MsG0180000243.01.T01:CDS
CAAGAACTTTGAATACACTC+TGG 0.530976 1:-3311882 None:intergenic
ACGATGCTCCGGCAAGGGTG+CGG 0.534124 1:-3308823 MsG0180000242.01.T01:intron
ATCTTCAATAGATTGAGTAC+TGG 0.534306 1:-3310689 None:intergenic
AGCGGTGGTTAGGGTTATGA+GGG 0.535461 1:-3308873 None:intergenic
TGCTAACAAGGAAACGTTCA+CGG 0.541569 1:-3312048 None:intergenic
GGAATTTAAAGATCACGAAC+TGG 0.544269 1:+3312099 MsG0180000243.01.T01:CDS
ATTGAGTACTGGATGGCTGC+CGG 0.547630 1:-3310678 None:intergenic
GATTCCAATGAAATTGTATG+GGG 0.567472 1:+3312239 MsG0180000243.01.T01:three_prime_UTR
CAACAACGACAATTGGGAAG+AGG 0.569094 1:+3308918 MsG0180000243.01.T01:CDS
TATCGATGTTGTTGATGAGC+AGG 0.570455 1:+3309041 MsG0180000243.01.T01:CDS
AGTACTGGATGGCTGCCGGC+CGG 0.572496 1:-3310674 None:intergenic
AGATCAGTTAGTAGTGAGTG+AGG 0.574644 1:+3312203 MsG0180000243.01.T01:exon
ACACACAGTTCAATTGGTGC+TGG 0.584222 1:+3311731 MsG0180000243.01.T01:CDS
GAAGTGCTTCGGTTGTAAGA+GGG 0.584506 1:-3312165 None:intergenic
TGACAACGATGCTCCGGCAA+GGG 0.585301 1:-3308828 None:intergenic
ATTCACAATATTGATGGACC+AGG 0.597689 1:+3310772 MsG0180000243.01.T01:CDS
ATGCTGCTATGTATCCTGAA+TGG 0.598898 1:+3309220 MsG0180000243.01.T01:CDS
GTTGTATGAAGTGGAAGTAG+AGG 0.603568 1:+3310723 MsG0180000243.01.T01:CDS
AAGAACTTTGAATACACTCT+GGG 0.606048 1:-3311881 None:intergenic
TCAATAGATTGAGTACTGGA+TGG 0.610328 1:-3310685 None:intergenic
GTGCGGTTGCGGATTTAGAG+AGG 0.613549 1:-3308806 MsG0180000242.01.T01:intron
CATGCCTTCAATCTTATATG+GGG 0.613591 1:-3311773 None:intergenic
CTGCCGTGTGCAAGAATCAG+AGG 0.614174 1:-3311802 None:intergenic
CTGACAACGATGCTCCGGCA+AGG 0.614196 1:-3308829 None:intergenic
GTGCAAGCAATCTTGACCAT+CGG 0.614626 1:-3312140 None:intergenic
AATACGAATATGGGTACAAG+AGG 0.617434 1:-3310836 None:intergenic
AGCATTAACATGGTCAATGG+AGG 0.619176 1:-3310863 None:intergenic
TTCGAAAATCGAAAGAGCGG+TGG 0.623444 1:-3308888 None:intergenic
TACAAAGGGGAGCATTAACA+TGG 0.630431 1:-3310873 None:intergenic
GTTGCGGATTTAGAGAGGTG+AGG 0.631772 1:-3308801 MsG0180000242.01.T01:intron
AATGAGAATTCAGAGTAACC+TGG 0.635096 1:-3312072 None:intergenic
TCAACCCGATAATAAAATGG+CGG 0.636810 1:+3308760 None:intergenic
TCAAATGAAAGCTAAACGCA+AGG 0.651514 1:+3310648 MsG0180000243.01.T01:CDS
TACAAGAGGCCAGACGAGCG+AGG 0.653242 1:-3310822 None:intergenic
TATCCTCTGATTCTTGCACA+CGG 0.655621 1:+3311799 MsG0180000243.01.T01:CDS
AAGACCAAGAGGCATTCCGA+TGG 0.661202 1:+3312124 MsG0180000243.01.T01:CDS
TCTTTCGAAAATCGAAAGAG+CGG 0.669490 1:-3308891 None:intergenic
CTAAACGCAAGGTTTCCGGC+CGG 0.673224 1:+3310659 MsG0180000243.01.T01:CDS
ATTCCAATGAAATTGTATGG+GGG 0.695481 1:+3312240 MsG0180000243.01.T01:three_prime_UTR
ACGAACTGGTCAAGACCAAG+AGG 0.697181 1:+3312113 MsG0180000243.01.T01:CDS
ACTGAGTAGAGATCACTGAT+CGG 0.699405 1:-3312022 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTGATCAATTATTATTAT+TGG - Chr1:3311436-3311455 None:intergenic 10.0%
!!! TTCAATAACATATTTTAAAA+TGG - Chr1:3311076-3311095 None:intergenic 10.0%
!!! TTTTTTCTTTTTAACATATT+AGG + Chr1:3310594-3310613 MsG0180000243.01.T01:intron 10.0%
!! AATAAACATAGAATTTGAAA+TGG + Chr1:3309925-3309944 MsG0180000243.01.T01:intron 15.0%
!! CAAAACTATTAAAAATGATT+TGG + Chr1:3311504-3311523 MsG0180000243.01.T01:intron 15.0%
!!! TAAGTTTTTATCATTTTACT+TGG + Chr1:3311936-3311955 MsG0180000243.01.T01:intron 15.0%
!!! TTTTATTATGCAAAGAATTA+GGG - Chr1:3309780-3309799 None:intergenic 15.0%
!! AATGTAATGTGATTTATGTT+TGG + Chr1:3309401-3309420 MsG0180000243.01.T01:intron 20.0%
!! TGTACTTAAAATAGCTTATT+TGG - Chr1:3310158-3310177 None:intergenic 20.0%
!! TTCAAATTCTATGTTTATTG+TGG - Chr1:3309924-3309943 None:intergenic 20.0%
!!! CTTTTATTATGCAAAGAATT+AGG - Chr1:3309781-3309800 None:intergenic 20.0%
!!! GATTTTGTAAAATTGATTCT+GGG + Chr1:3309365-3309384 MsG0180000243.01.T01:intron 20.0%
!!! TAAATTGTGTGTGAATTTTT+TGG - Chr1:3310082-3310101 None:intergenic 20.0%
!!! TGATTTTGTAAAATTGATTC+TGG + Chr1:3309364-3309383 MsG0180000243.01.T01:intron 20.0%
!!! TTTATTTTCTGTTGAATTTC+TGG + Chr1:3309093-3309112 MsG0180000243.01.T01:intron 20.0%
!!! TTTTGATTTTGTATGTGATT+AGG + Chr1:3309128-3309147 MsG0180000243.01.T01:intron 20.0%
! AAACATGTAATATAGCTCTT+TGG + Chr1:3310492-3310511 MsG0180000243.01.T01:intron 25.0%
! AAGTATATGTGACTATATGA+AGG + Chr1:3311634-3311653 MsG0180000243.01.T01:intron 25.0%
! ATGATTCCAATGAAATTGTA+TGG + Chr1:3312237-3312256 MsG0180000243.01.T01:three_prime_UTR 25.0%
! ATTCTGTAGTTGTCATTAAT+GGG + Chr1:3310408-3310427 MsG0180000243.01.T01:CDS 25.0%
! CCATATCTAAAGATGAAATA+TGG - Chr1:3310971-3310990 None:intergenic 25.0%
! CCATATTTCATCTTTAGATA+TGG + Chr1:3310968-3310987 MsG0180000243.01.T01:intron 25.0%
! CTAAACATGAAAAGGAAATT+GGG + Chr1:3309181-3309200 MsG0180000243.01.T01:CDS 25.0%
! GTAGTAATTCACAATATTGA+TGG + Chr1:3310766-3310785 MsG0180000243.01.T01:CDS 25.0%
! TATTCAACCTAAACATGAAA+AGG + Chr1:3309173-3309192 MsG0180000243.01.T01:CDS 25.0%
! TATTCTGTAGTTGTCATTAA+TGG + Chr1:3310407-3310426 MsG0180000243.01.T01:CDS 25.0%
! TGATAAAAACTTACCTTCAT+CGG - Chr1:3311929-3311948 None:intergenic 25.0%
! TGATTCCAATGAAATTGTAT+GGG + Chr1:3312238-3312257 MsG0180000243.01.T01:three_prime_UTR 25.0%
! TTATACAGAGCTAAAAACTT+AGG - Chr1:3311154-3311173 None:intergenic 25.0%
! TTCTTATCATTCAACACTAA+TGG + Chr1:3309274-3309293 MsG0180000243.01.T01:intron 25.0%
!! AACATAGAATTTGAAATGGA+TGG + Chr1:3309929-3309948 MsG0180000243.01.T01:intron 25.0%
!! ACATAGAATTTGAAATGGAT+GGG + Chr1:3309930-3309949 MsG0180000243.01.T01:intron 25.0%
!! TTAACAGTTACATCTTCTTT+TGG + Chr1:3311684-3311703 MsG0180000243.01.T01:intron 25.0%
!!! AAGAAACTATTTTCTAGCAA+AGG + Chr1:3308975-3308994 MsG0180000243.01.T01:CDS 25.0%
!!! AGAATTGATTTTGACATGTT+TGG - Chr1:3309455-3309474 None:intergenic 25.0%
!!! ATCTGTTTGAGATTTCTTTT+GGG + Chr1:3311026-3311045 MsG0180000243.01.T01:intron 25.0%
!!! CTTGGTTTTGATAGATATAA+TGG + Chr1:3311556-3311575 MsG0180000243.01.T01:intron 25.0%
!!! GTGCATATGACATAATTTTT+TGG - Chr1:3309621-3309640 None:intergenic 25.0%
!!! GTGCATTTTAAGTTTTACAT+TGG + Chr1:3310266-3310285 MsG0180000243.01.T01:intron 25.0%
!!! GTTCTAAAGTAAGAAGATAA+AGG - Chr1:3309878-3309897 None:intergenic 25.0%
!!! TATCTGTTTGAGATTTCTTT+TGG + Chr1:3311025-3311044 MsG0180000243.01.T01:intron 25.0%
!!! TATTTTTGCTGTTTATCACT+GGG + Chr1:3311109-3311128 MsG0180000243.01.T01:intron 25.0%
!!! TGTGTGTGAATTTTTTGTAA+GGG - Chr1:3310077-3310096 None:intergenic 25.0%
!!! TTATCTTCTTACTTTAGAAC+CGG + Chr1:3309878-3309897 MsG0180000243.01.T01:intron 25.0%
!!! TTATTTTTGCTGTTTATCAC+TGG + Chr1:3311108-3311127 MsG0180000243.01.T01:intron 25.0%
!!! TTGTGTGTGAATTTTTTGTA+AGG - Chr1:3310078-3310097 None:intergenic 25.0%
AACATGCCTTCAATCTTATA+TGG - Chr1:3311778-3311797 None:intergenic 30.0%
AACTCATATTCGCTTATAAG+TGG + Chr1:3309679-3309698 MsG0180000243.01.T01:intron 30.0%
AAGAACTTTGAATACACTCT+GGG - Chr1:3311884-3311903 None:intergenic 30.0%
AATAGTTAGAATAGCAAGTC+CGG - Chr1:3309900-3309919 None:intergenic 30.0%
AATTTCTTAGTGAAGGATCT+CGG + Chr1:3310548-3310567 MsG0180000243.01.T01:intron 30.0%
ACAGATGAATTTCTTAGTGA+AGG + Chr1:3310541-3310560 MsG0180000243.01.T01:intron 30.0%
ACATGCCTTCAATCTTATAT+GGG - Chr1:3311777-3311796 None:intergenic 30.0%
ACTCATATTCGCTTATAAGT+GGG + Chr1:3309680-3309699 MsG0180000243.01.T01:intron 30.0%
ACTTTCCGAAATTGTATGAT+AGG + Chr1:3312286-3312305 MsG0180000243.01.T01:three_prime_UTR 30.0%
AGTGAAAAGTTAAGGCAAAA+TGG + Chr1:3310516-3310535 MsG0180000243.01.T01:intron 30.0%
ATCTATCAAAACCAAGACTT+GGG - Chr1:3311552-3311571 None:intergenic 30.0%
ATCTTCAATAGATTGAGTAC+TGG - Chr1:3310692-3310711 None:intergenic 30.0%
ATGTCGTCTATAAGATAGTT+TGG + Chr1:3311394-3311413 MsG0180000243.01.T01:intron 30.0%
ATTCCAATGAAATTGTATGG+GGG + Chr1:3312240-3312259 MsG0180000243.01.T01:three_prime_UTR 30.0%
CAGTAACTAAACTTACAAAG+GGG - Chr1:3310889-3310908 None:intergenic 30.0%
CCTAAACATGAAAAGGAAAT+TGG + Chr1:3309180-3309199 MsG0180000243.01.T01:CDS 30.0%
CTCAAGACGAAGAAAATATT+TGG - Chr1:3311224-3311243 None:intergenic 30.0%
CTGTTAAGAAAAATAGTCAG+AGG - Chr1:3311671-3311690 None:intergenic 30.0%
GAAAAGAGAGTAATACCTTA+GGG - Chr1:3309253-3309272 None:intergenic 30.0%
GATAAAAACTTACCTTCATC+GGG - Chr1:3311928-3311947 None:intergenic 30.0%
GATATCAGTAATCTTGTGTT+TGG - Chr1:3309025-3309044 None:intergenic 30.0%
GATGGCATATATTAAGCAAA+AGG + Chr1:3311357-3311376 MsG0180000243.01.T01:intron 30.0%
GATTCCAATGAAATTGTATG+GGG + Chr1:3312239-3312258 MsG0180000243.01.T01:three_prime_UTR 30.0%
GCAGTAACTAAACTTACAAA+GGG - Chr1:3310890-3310909 None:intergenic 30.0%
TACTCTGAATTCTCATTTGA+CGG + Chr1:3312078-3312097 MsG0180000243.01.T01:CDS 30.0%
TATCTATCAAAACCAAGACT+TGG - Chr1:3311553-3311572 None:intergenic 30.0%
TCAGACCATTAACTTAAAAC+AGG + Chr1:3310436-3310455 MsG0180000243.01.T01:CDS 30.0%
TCTTACATTGTTGTATGAAG+TGG + Chr1:3310714-3310733 MsG0180000243.01.T01:CDS 30.0%
TGAAAAGAGAGTAATACCTT+AGG - Chr1:3309254-3309273 None:intergenic 30.0%
! AGCTATATTACATGTTTCTG+TGG - Chr1:3310489-3310508 None:intergenic 30.0%
! ATATTAGGTTCTGATAGCTT+TGG + Chr1:3310609-3310628 MsG0180000243.01.T01:intron 30.0%
! CCAATTTCCTTTTCATGTTT+AGG - Chr1:3309183-3309202 None:intergenic 30.0%
!! TCTTTGGTAGTGAAAAGTTA+AGG + Chr1:3310508-3310527 MsG0180000243.01.T01:intron 30.0%
!!! AGTTTTTAGCTCTGTATAAC+TGG + Chr1:3311155-3311174 MsG0180000243.01.T01:intron 30.0%
!!! GCAAACCTGTTTTAAGTTAA+TGG - Chr1:3310444-3310463 None:intergenic 30.0%
!!! TAGAACTTTTTGAAGTGCTT+CGG - Chr1:3312179-3312198 None:intergenic 30.0%
AAACGACTCTTGAAATCTTG+TGG + Chr1:3311595-3311614 MsG0180000243.01.T01:intron 35.0%
AACTGGACAATAGGACTAAA+TGG + Chr1:3311172-3311191 MsG0180000243.01.T01:intron 35.0%
AAGTATGTTATGCGTGTGTT+TGG + Chr1:3309323-3309342 MsG0180000243.01.T01:intron 35.0%
AATACGAATATGGGTACAAG+AGG - Chr1:3310839-3310858 None:intergenic 35.0%
AATGAGAATTCAGAGTAACC+TGG - Chr1:3312075-3312094 None:intergenic 35.0%
ACAAACACTTACTGCATCTT+TGG - Chr1:3309590-3309609 None:intergenic 35.0%
AGATGAACAACAACGACAAT+TGG + Chr1:3308911-3308930 MsG0180000243.01.T01:exon 35.0%
ATAGCACATACTTCTTACTC+CGG - Chr1:3309974-3309993 None:intergenic 35.0%
ATCATTGACGTAGGCATAAT+TGG + Chr1:3309819-3309838 MsG0180000243.01.T01:intron 35.0%
ATCTTATATGGGGCAAATGT+AGG - Chr1:3311766-3311785 None:intergenic 35.0%
ATGGATGGGAAATGAAATCA+AGG + Chr1:3309944-3309963 MsG0180000243.01.T01:intron 35.0%
ATTAAGCAAAAGGTGGTACT+TGG + Chr1:3311367-3311386 MsG0180000243.01.T01:intron 35.0%
ATTTCTTAGTGAAGGATCTC+GGG + Chr1:3310549-3310568 MsG0180000243.01.T01:intron 35.0%
CAAATATTTAGTCTCCTGTC+AGG - Chr1:3312353-3312372 None:intergenic 35.0%
CAAGAACTTTGAATACACTC+TGG - Chr1:3311885-3311904 None:intergenic 35.0%
CATGACTAATCGTTATGTTC+TGG + Chr1:3309850-3309869 MsG0180000243.01.T01:intron 35.0%
CATGCCTTCAATCTTATATG+GGG - Chr1:3311776-3311795 None:intergenic 35.0%
CGAAGCCTATCATACAATTT+CGG - Chr1:3312294-3312313 None:intergenic 35.0%
CTTGTGTTTGGATTTCTTCA+CGG - Chr1:3309013-3309032 None:intergenic 35.0%
GAAATGAAATCAAGGTGAAC+CGG + Chr1:3309952-3309971 MsG0180000243.01.T01:intron 35.0%
GATAGGCTTCGTATATTGAA+TGG + Chr1:3312303-3312322 MsG0180000243.01.T01:three_prime_UTR 35.0%
GATGAACAACAACGACAATT+GGG + Chr1:3308912-3308931 MsG0180000243.01.T01:exon 35.0%
GCAAGTTGTTAAAGTTGAGA+TGG + Chr1:3309719-3309738 MsG0180000243.01.T01:intron 35.0%
GGAAGGGAAAAAATACAGTT+AGG - Chr1:3311995-3312014 None:intergenic 35.0%
GGAATTTAAAGATCACGAAC+TGG + Chr1:3312099-3312118 MsG0180000243.01.T01:CDS 35.0%
GGCAGTAACTAAACTTACAA+AGG - Chr1:3310891-3310910 None:intergenic 35.0%
GGCATATATTAAGCAAAAGG+TGG + Chr1:3311360-3311379 MsG0180000243.01.T01:intron 35.0%
GGCTCATTCAAAAAAAGTGA+AGG - Chr1:3310303-3310322 None:intergenic 35.0%
GTCTATAAGATAGTTTGGTC+AGG + Chr1:3311399-3311418 MsG0180000243.01.T01:intron 35.0%
TAACAACTTGCAATGCTAGA+GGG - Chr1:3309710-3309729 None:intergenic 35.0%
TCAAATGAAAGCTAAACGCA+AGG + Chr1:3310648-3310667 MsG0180000243.01.T01:CDS 35.0%
TCTGCTTTACAGTTGTTTGT+AGG + Chr1:3311278-3311297 MsG0180000243.01.T01:intron 35.0%
TCTTTCGAAAATCGAAAGAG+CGG - Chr1:3308894-3308913 None:intergenic 35.0%
TGACTACATTTGTGAAAGAC+AGG + Chr1:3311328-3311347 MsG0180000243.01.T01:intron 35.0%
TTAACAACTTGCAATGCTAG+AGG - Chr1:3309711-3309730 None:intergenic 35.0%
TTTGCCCCATATAAGATTGA+AGG + Chr1:3311769-3311788 MsG0180000243.01.T01:CDS 35.0%
! ACATCTTCTTTTGGCAGTTT+GGG + Chr1:3311693-3311712 MsG0180000243.01.T01:intron 35.0%
! AGAAGTATGTGCTATGTAAG+CGG + Chr1:3309979-3309998 MsG0180000243.01.T01:intron 35.0%
! ATATTGATGGACCAGGATTA+AGG + Chr1:3310779-3310798 MsG0180000243.01.T01:CDS 35.0%
! ATGCTGTTCTTTTCACATGT+AGG + Chr1:3311966-3311985 MsG0180000243.01.T01:intron 35.0%
! ATTCACAATATTGATGGACC+AGG + Chr1:3310772-3310791 MsG0180000243.01.T01:CDS 35.0%
! GGTTCTACAGCTTTCTAAAA+GGG - Chr1:3311203-3311222 None:intergenic 35.0%
! TACATCTTCTTTTGGCAGTT+TGG + Chr1:3311692-3311711 MsG0180000243.01.T01:intron 35.0%
! TATTGATGGACCAGGATTAA+GGG + Chr1:3310780-3310799 MsG0180000243.01.T01:CDS 35.0%
! TGGTTCTACAGCTTTCTAAA+AGG - Chr1:3311204-3311223 None:intergenic 35.0%
!! TCAATAGATTGAGTACTGGA+TGG - Chr1:3310688-3310707 None:intergenic 35.0%
!!! AACTGTATTTTTTCCCTTCC+AGG + Chr1:3311995-3312014 MsG0180000243.01.T01:intron 35.0%
!!! AATTTTGACTCCTCCAAAAG+TGG + Chr1:3309562-3309581 MsG0180000243.01.T01:intron 35.0%
!!! TTTTTTGAATGAGCCAGATG+CGG + Chr1:3310308-3310327 MsG0180000243.01.T01:intron 35.0%
AAAACCAAGACTTGGGACTT+GGG - Chr1:3311545-3311564 None:intergenic 40.0%
AAAGCTAAACGCAAGGTTTC+CGG + Chr1:3310655-3310674 MsG0180000243.01.T01:CDS 40.0%
ACTGAGTAGAGATCACTGAT+CGG - Chr1:3312025-3312044 None:intergenic 40.0%
AGATCAGTTAGTAGTGAGTG+AGG + Chr1:3312203-3312222 MsG0180000243.01.T01:exon 40.0%
AGCATTAACATGGTCAATGG+AGG - Chr1:3310866-3310885 None:intergenic 40.0%
ATGCTGCTATGTATCCTGAA+TGG + Chr1:3309220-3309239 MsG0180000243.01.T01:CDS 40.0%
CAAATTTCTCATCCCGATGA+AGG + Chr1:3311913-3311932 MsG0180000243.01.T01:CDS 40.0%
CTACGTCAATGATAGCAAAG+TGG - Chr1:3309812-3309831 None:intergenic 40.0%
CTGTGGAATGCAAGATGTTA+GGG - Chr1:3310472-3310491 None:intergenic 40.0%
CTTGAAATCTTGTGGCAGTA+GGG + Chr1:3311603-3311622 MsG0180000243.01.T01:intron 40.0%
GCTCTGTATAACTGGACAAT+AGG + Chr1:3311163-3311182 MsG0180000243.01.T01:intron 40.0%
GGAAGAAGAAGAAGAAGAAG+AGG + Chr1:3308939-3308958 MsG0180000243.01.T01:CDS 40.0%
GGAGGCAACAATACGAATAT+GGG - Chr1:3310848-3310867 None:intergenic 40.0%
GGGTGCTTGAACTTTGAATT+GGG + Chr1:3311046-3311065 MsG0180000243.01.T01:intron 40.0%
GTTGTATGAAGTGGAAGTAG+AGG + Chr1:3310723-3310742 MsG0180000243.01.T01:CDS 40.0%
TATCCTCTGATTCTTGCACA+CGG + Chr1:3311799-3311818 MsG0180000243.01.T01:CDS 40.0%
TCTGTGGAATGCAAGATGTT+AGG - Chr1:3310473-3310492 None:intergenic 40.0%
TCTTGAAATCTTGTGGCAGT+AGG + Chr1:3311602-3311621 MsG0180000243.01.T01:intron 40.0%
TGCTAACAAGGAAACGTTCA+CGG - Chr1:3312051-3312070 None:intergenic 40.0%
TGGAGGCAACAATACGAATA+TGG - Chr1:3310849-3310868 None:intergenic 40.0%
TGGGTGCTTGAACTTTGAAT+TGG + Chr1:3311045-3311064 MsG0180000243.01.T01:intron 40.0%
TTCTGCACGAGATAATCTGA+CGG - Chr1:3310912-3310931 None:intergenic 40.0%
TTGAATACACTCTGGGCATT+GGG - Chr1:3311877-3311896 None:intergenic 40.0%
TTGCCCCCATACAATTTCAT+TGG - Chr1:3312246-3312265 None:intergenic 40.0%
TTTGAATACACTCTGGGCAT+TGG - Chr1:3311878-3311897 None:intergenic 40.0%
! CACTTTGCTATCATTGACGT+AGG + Chr1:3309810-3309829 MsG0180000243.01.T01:intron 40.0%
! GCTTGCAGAATTGATTCTAG+AGG - Chr1:3309481-3309500 None:intergenic 40.0%
! TATCGATGTTGTTGATGAGC+AGG + Chr1:3309041-3309060 MsG0180000243.01.T01:CDS 40.0%
! TGGTGTATCTAGAGTGTGTT+TGG + Chr1:3309294-3309313 MsG0180000243.01.T01:intron 40.0%
!! ATCTTTGGTTCCACTTTTGG+AGG - Chr1:3309575-3309594 None:intergenic 40.0%
!! GTATGGCTTTGGATCTAAGA+AGG + Chr1:3310346-3310365 MsG0180000243.01.T01:CDS 40.0%
!! TACAAAGGGGAGCATTAACA+TGG - Chr1:3310876-3310895 None:intergenic 40.0%
!! TGCATCTTTGGTTCCACTTT+TGG - Chr1:3309578-3309597 None:intergenic 40.0%
!!! TTGCAGCTGTTTTGACACTT+TGG - Chr1:3311834-3311853 None:intergenic 40.0%
AAATCGAAAGAGCGGTGGTT+AGG - Chr1:3308886-3308905 None:intergenic 45.0%
AATCGAAAGAGCGGTGGTTA+GGG - Chr1:3308885-3308904 None:intergenic 45.0%
ACACACAGTTCAATTGGTGC+TGG + Chr1:3311731-3311750 MsG0180000243.01.T01:CDS 45.0%
CAAAACCAAGACTTGGGACT+TGG - Chr1:3311546-3311565 None:intergenic 45.0%
CAACAACGACAATTGGGAAG+AGG + Chr1:3308918-3308937 MsG0180000243.01.T01:CDS 45.0%
GCTCTTCTGATGTATGGCTT+TGG + Chr1:3310335-3310354 MsG0180000243.01.T01:CDS 45.0%
GGATTCGCTCTTCTGATGTA+TGG + Chr1:3310329-3310348 MsG0180000243.01.T01:CDS 45.0%
GTGCAAGCAATCTTGACCAT+CGG - Chr1:3312143-3312162 None:intergenic 45.0%
GTTTCAGAGTCCCTTAATCC+TGG - Chr1:3310793-3310812 None:intergenic 45.0%
TGGCTCACACACAGTTCAAT+TGG + Chr1:3311725-3311744 MsG0180000243.01.T01:CDS 45.0%
TTATCACTGGGTGTTCTCTG+AGG + Chr1:3311121-3311140 MsG0180000243.01.T01:intron 45.0%
TTCGAAAATCGAAAGAGCGG+TGG - Chr1:3308891-3308910 None:intergenic 45.0%
! ATCTGAAGCTCATTCCTGAC+AGG + Chr1:3312336-3312355 MsG0180000243.01.T01:three_prime_UTR 45.0%
! GAAGTGCTTCGGTTGTAAGA+GGG - Chr1:3312168-3312187 None:intergenic 45.0%
! GGCAGTTTGGGACAAGAATA+TGG + Chr1:3311705-3311724 MsG0180000243.01.T01:intron 45.0%
! GTGAAAGACAGGAAGTACGA+TGG + Chr1:3311339-3311358 MsG0180000243.01.T01:intron 45.0%
! TGAAGTGCTTCGGTTGTAAG+AGG - Chr1:3312169-3312188 None:intergenic 45.0%
!! GGGAGCATTAACATGGTCAA+TGG - Chr1:3310869-3310888 None:intergenic 45.0%
!!! AGCTGTTTTGACACTTTGGC+TGG - Chr1:3311830-3311849 None:intergenic 45.0%
ACGAACTGGTCAAGACCAAG+AGG + Chr1:3312113-3312132 MsG0180000243.01.T01:CDS 50.0%
ACGTTTCCTTGTTAGCAGCC+AGG + Chr1:3312054-3312073 MsG0180000243.01.T01:CDS 50.0%
ACTGATCGGCATTCCTGGAA+GGG - Chr1:3312011-3312030 None:intergenic 50.0%
AGATCACTGATCGGCATTCC+TGG - Chr1:3312016-3312035 None:intergenic 50.0%
AGCGGTGGTTAGGGTTATGA+GGG - Chr1:3308876-3308895 None:intergenic 50.0%
CTGAATGGCTTCTCTCCCTA+AGG + Chr1:3309235-3309254 MsG0180000243.01.T01:CDS 50.0%
CTTAGGGAGAGAAGCCATTC+AGG - Chr1:3309237-3309256 None:intergenic 50.0%
GAGTAACCTGGCTGCTAACA+AGG - Chr1:3312063-3312082 None:intergenic 50.0%
GTTGCGGATTTAGAGAGGTG+AGG - Chr1:3308804-3308823 MsG0180000242.01.T01:intron 50.0%
TTGACCATCGGAATGCCTCT+TGG - Chr1:3312131-3312150 None:intergenic 50.0%
!! AAGACCAAGAGGCATTCCGA+TGG + Chr1:3312124-3312143 MsG0180000243.01.T01:CDS 50.0%
!! ATTGAGTACTGGATGGCTGC+CGG - Chr1:3310681-3310700 None:intergenic 50.0%
!! CAGACGAGCGAGGTATTGTT+GGG - Chr1:3310815-3310834 None:intergenic 50.0%
CACTGATCGGCATTCCTGGA+AGG - Chr1:3312012-3312031 None:intergenic 55.0%
CAGAAGAGCGAATCCGCATC+TGG - Chr1:3310324-3310343 None:intergenic 55.0%
CCAACAATACCTCGCTCGTC+TGG + Chr1:3310813-3310832 MsG0180000243.01.T01:CDS 55.0%
CGCTACTGACAACGATGCTC+CGG - Chr1:3308837-3308856 None:intergenic 55.0%
CTAAACGCAAGGTTTCCGGC+CGG + Chr1:3310659-3310678 MsG0180000243.01.T01:CDS 55.0%
CTGCCGTGTGCAAGAATCAG+AGG - Chr1:3311805-3311824 None:intergenic 55.0%
GAGCGGTGGTTAGGGTTATG+AGG - Chr1:3308877-3308896 None:intergenic 55.0%
GTGCGGTTGCGGATTTAGAG+AGG - Chr1:3308809-3308828 MsG0180000242.01.T01:intron 55.0%
TGACAACGATGCTCCGGCAA+GGG - Chr1:3308831-3308850 None:intergenic 55.0%
TGCTCCCAAGTCCCAAGTCT+TGG + Chr1:3311538-3311557 MsG0180000243.01.T01:intron 55.0%
!! CCAGACGAGCGAGGTATTGT+TGG - Chr1:3310816-3310835 None:intergenic 55.0%
CTGACAACGATGCTCCGGCA+AGG - Chr1:3308832-3308851 None:intergenic 60.0%
TACAAGAGGCCAGACGAGCG+AGG - Chr1:3310825-3310844 None:intergenic 60.0%
ACGATGCTCCGGCAAGGGTG+CGG - Chr1:3308826-3308845 None:intergenic 65.0%
ATCCGCAACCGCACCCTTGC+CGG + Chr1:3308815-3308834 MsG0180000243.01.T01:five_prime_UTR 65.0%
! AGTACTGGATGGCTGCCGGC+CGG - Chr1:3310677-3310696 None:intergenic 65.0%
! CTCCGGCAAGGGTGCGGTTG+CGG - Chr1:3308820-3308839 MsG0180000242.01.T01:intron 70.0%
Chromosome Type Strat End Strand Name
Chr1 gene 3308772 3312414 3308772 ID=MsG0180000243.01;Name=MsG0180000243.01
Chr1 mRNA 3308772 3312414 3308772 ID=MsG0180000243.01.T01;Parent=MsG0180000243.01;Name=MsG0180000243.01.T01;_AED=0.32;_eAED=0.32;_QI=141|1|1|1|1|1|6|201|362
Chr1 exon 3312017 3312414 3312017 ID=MsG0180000243.01.T01:exon:6198;Parent=MsG0180000243.01.T01
Chr1 exon 3311710 3311934 3311710 ID=MsG0180000243.01.T01:exon:6197;Parent=MsG0180000243.01.T01
Chr1 exon 3310616 3310892 3310616 ID=MsG0180000243.01.T01:exon:6196;Parent=MsG0180000243.01.T01
Chr1 exon 3310325 3310457 3310325 ID=MsG0180000243.01.T01:exon:6195;Parent=MsG0180000243.01.T01
Chr1 exon 3309150 3309256 3309150 ID=MsG0180000243.01.T01:exon:6194;Parent=MsG0180000243.01.T01
Chr1 exon 3308772 3309062 3308772 ID=MsG0180000243.01.T01:exon:6193;Parent=MsG0180000243.01.T01
Chr1 five_prime_UTR 3308772 3308912 3308772 ID=MsG0180000243.01.T01:five_prime_utr;Parent=MsG0180000243.01.T01
Chr1 CDS 3308913 3309062 3308913 ID=MsG0180000243.01.T01:cds;Parent=MsG0180000243.01.T01
Chr1 CDS 3309150 3309256 3309150 ID=MsG0180000243.01.T01:cds;Parent=MsG0180000243.01.T01
Chr1 CDS 3310325 3310457 3310325 ID=MsG0180000243.01.T01:cds;Parent=MsG0180000243.01.T01
Chr1 CDS 3310616 3310892 3310616 ID=MsG0180000243.01.T01:cds;Parent=MsG0180000243.01.T01
Chr1 CDS 3311710 3311934 3311710 ID=MsG0180000243.01.T01:cds;Parent=MsG0180000243.01.T01
Chr1 CDS 3312017 3312213 3312017 ID=MsG0180000243.01.T01:cds;Parent=MsG0180000243.01.T01
Chr1 three_prime_UTR 3312214 3312414 3312214 ID=MsG0180000243.01.T01:three_prime_utr;Parent=MsG0180000243.01.T01
Gene Sequence

>MsG0180000243.01.T01

ATGAACAACAACGACAATTGGGAAGAGGAAGAAGAAGAAGAAGAAGAGGATCTAGAATTCTCAAGAAACTATTTTCTAGCAAAGGAATTAACATCTTCCGTGAAGAAATCCAAACACAAGATTACTGATATCGATGTTGTTGATGAGCAGGAACTTAGAGAAGCTTCATTGCATATTCAACCTAAACATGAAAAGGAAATTGGGCTTTTAATCGATTCGTATGCTGCTATGTATCCTGAATGGCTTCTCTCCCTAAGATGCGGATTCGCTCTTCTGATGTATGGCTTTGGATCTAAGAAGGCTTTGATTGAAGACTTTGCTTCGAAGACATTGACAGAATATTCTGTAGTTGTCATTAATGGGTATCTTCAGACCATTAACTTAAAACAGGTTCTGATAGCTTTGGCTGAACTTCTATGTGATCAAATGAAAGCTAAACGCAAGGTTTCCGGCCGGCAGCCATCCAGTACTCAATCTATTGAAGATCTTCTTACATTGTTGTATGAAGTGGAAGTAGAGGACAGCAATTCTTTCGTTTGTGTAGTAATTCACAATATTGATGGACCAGGATTAAGGGACTCTGAAACCCAACAATACCTCGCTCGTCTGGCCTCTTGTACCCATATTCGTATTGTTGCCTCCATTGACCATGTTAATGCTCCCCTTTTTTGGGACAAGAATATGGCTCACACACAGTTCAATTGGTGCTGGCATCATGTTCCTACATTTGCCCCATATAAGATTGAAGGCATGTTTTATCCTCTGATTCTTGCACACGGCAGTGCCAGCCAAAGTGTCAAAACAGCTGCAATAGTTTTGCAAAGTTTGACACCCAATGCCCAGAGTGTATTCAAAGTTCTTGCTGAATATCAAATTTCTCATCCCGATGAAGGAATGCCGATCAGTGATCTCTACTCAGTCTGCCGTGAACGTTTCCTTGTTAGCAGCCAGGTTACTCTGAATTCTCATTTGACGGAATTTAAAGATCACGAACTGGTCAAGACCAAGAGGCATTCCGATGGTCAAGATTGCTTGCACATCCCTCTTACAACCGAAGCACTTCAAAAAGTTCTACTTGAGATCAGTTAG
Protein sequence

>MsG0180000243.01.T01

MNNNDNWEEEEEEEEEDLEFSRNYFLAKELTSSVKKSKHKITDIDVVDEQELREASLHIQPKHEKEIGLLIDSYAAMYPEWLLSLRCGFALLMYGFGSKKALIEDFASKTLTEYSVVVINGYLQTINLKQVLIALAELLCDQMKAKRKVSGRQPSSTQSIEDLLTLLYEVEVEDSNSFVCVVIHNIDGPGLRDSETQQYLARLASCTHIRIVASIDHVNAPLFWDKNMAHTQFNWCWHHVPTFAPYKIEGMFYPLILAHGSASQSVKTAAIVLQSLTPNAQSVFKVLAEYQISHPDEGMPISDLYSVCRERFLVSSQVTLNSHLTEFKDHELVKTKRHSDGQDCLHIPLTTEALQKVLLEIS*