Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021236.01.T01 | KEH30432.1 | 93.671 | 79 | 5 | 0 | 15 | 93 | 392 | 470 | 9.49E-49 | 171 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021236.01.T01 | B9G9L9 | 48.571 | 70 | 34 | 2 | 14 | 83 | 514 | 581 | 1.71E-17 | 79 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021236.01.T01 | A0A072UL76 | 93.671 | 79 | 5 | 0 | 15 | 93 | 392 | 470 | 4.53e-49 | 171 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048134.01 | MsG0480021236.01 | 0.809773 | 1.603150e-50 | 9.497242e-48 |
| MsG0180003320.01 | MsG0480021236.01 | 0.825650 | 4.176453e-54 | 3.829784e-51 |
| MsG0180003372.01 | MsG0480021236.01 | 0.808760 | 2.644096e-50 | 1.524543e-47 |
| MsG0180003504.01 | MsG0480021236.01 | 0.808741 | 2.668025e-50 | 1.537547e-47 |
| MsG0380012889.01 | MsG0480021236.01 | 0.806534 | 7.851332e-50 | 4.271952e-47 |
| MsG0480018559.01 | MsG0480021236.01 | 0.810765 | 9.797914e-51 | 5.957938e-48 |
| MsG0480020904.01 | MsG0480021236.01 | 0.824619 | 7.317528e-54 | 6.516372e-51 |
| MsG0480021236.01 | MsG0480022833.01 | 0.823259 | 1.525046e-53 | 1.306457e-50 |
| MsG0480021236.01 | MsG0480023432.01 | 0.806638 | 7.463165e-50 | 4.071348e-47 |
| MsG0480021236.01 | MsG0580024207.01 | 0.808216 | 3.454063e-50 | 1.963524e-47 |
| MsG0480021236.01 | MsG0580026521.01 | 0.825762 | 3.927474e-54 | 3.613124e-51 |
| MsG0480021236.01 | MsG0580028577.01 | 0.806932 | 6.469018e-50 | 3.556090e-47 |
| MsG0480021236.01 | MsG0680030613.01 | 0.828222 | 1.013491e-54 | 1.001075e-51 |
| MsG0480021236.01 | MsG0680032932.01 | 0.807404 | 5.139078e-50 | 2.859936e-47 |
| MsG0480021236.01 | MsG0680034238.01 | 0.836149 | 1.108313e-56 | 1.384671e-53 |
| MsG0480021236.01 | MsG0780035930.01 | 0.806972 | 6.344788e-50 | 3.491370e-47 |
| MsG0480021236.01 | MsG0780038657.01 | 0.811987 | 5.316869e-51 | 3.339533e-48 |
| MsG0480021236.01 | MsG0780040716.01 | 0.827314 | 1.675152e-54 | 1.611476e-51 |
| MsG0480021236.01 | MsG0780040719.01 | 0.805623 | 1.220795e-49 | 6.489427e-47 |
| MsG0480021236.01 | MsG0780040991.01 | 0.807785 | 4.264879e-50 | 2.397044e-47 |
| MsG0480021236.01 | MsG0880042642.01 | 0.808955 | 2.401957e-50 | 1.392114e-47 |
| MsG0480021236.01 | MsG0880044712.01 | 0.802477 | 5.508084e-49 | 2.700752e-46 |
| MsG0480021236.01 | MsG0880046870.01 | 0.812644 | 3.821676e-51 | 2.443144e-48 |
| MsG0480021236.01 | MsG0880047450.01 | 0.806575 | 7.697198e-50 | 4.192375e-47 |
| MsG0380017901.01 | MsG0480021236.01 | 0.839247 | 1.775860e-57 | 2.438001e-54 |
| MsG0280009432.01 | MsG0480021236.01 | 0.804597 | 2.001494e-49 | 1.036198e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021236.01.T01 | MTR_4g069550 | 93.671 | 79 | 5 | 0 | 15 | 93 | 392 | 470 | 1.15e-52 | 171 |
| MsG0480021236.01.T01 | MTR_4g069520 | 89.873 | 79 | 8 | 0 | 15 | 93 | 638 | 716 | 5.37e-51 | 170 |
| MsG0480021236.01.T01 | MTR_4g069520 | 89.873 | 79 | 8 | 0 | 15 | 93 | 652 | 730 | 5.98e-51 | 170 |
| MsG0480021236.01.T01 | MTR_4g068420 | 74.359 | 78 | 20 | 0 | 16 | 93 | 51 | 128 | 1.09e-42 | 135 |
| MsG0480021236.01.T01 | MTR_4g069570 | 81.690 | 71 | 13 | 0 | 16 | 86 | 266 | 336 | 2.10e-42 | 141 |
| MsG0480021236.01.T01 | MTR_4g069040 | 82.895 | 76 | 13 | 0 | 16 | 91 | 440 | 515 | 8.73e-42 | 143 |
| MsG0480021236.01.T01 | MTR_6g086530 | 70.000 | 70 | 21 | 0 | 16 | 85 | 136 | 205 | 2.54e-35 | 119 |
| MsG0480021236.01.T01 | MTR_3g116270 | 57.534 | 73 | 29 | 2 | 16 | 88 | 438 | 508 | 1.89e-26 | 101 |
| MsG0480021236.01.T01 | MTR_3g116320 | 58.904 | 73 | 28 | 2 | 16 | 88 | 620 | 690 | 3.96e-26 | 100 |
| MsG0480021236.01.T01 | MTR_3g116410 | 49.351 | 77 | 37 | 2 | 16 | 92 | 208 | 282 | 9.34e-21 | 83.6 |
| MsG0480021236.01.T01 | MTR_3g107830 | 45.570 | 79 | 41 | 2 | 14 | 92 | 186 | 262 | 3.70e-20 | 81.6 |
| MsG0480021236.01.T01 | MTR_3g116380 | 46.479 | 71 | 36 | 2 | 16 | 86 | 235 | 303 | 2.27e-18 | 77.4 |
| MsG0480021236.01.T01 | MTR_4g069680 | 47.222 | 72 | 36 | 2 | 14 | 85 | 217 | 286 | 1.00e-17 | 75.9 |
| MsG0480021236.01.T01 | MTR_3g109490 | 49.412 | 85 | 30 | 5 | 11 | 85 | 240 | 321 | 6.87e-17 | 73.6 |
| MsG0480021236.01.T01 | MTR_1g008500 | 41.463 | 82 | 37 | 4 | 11 | 84 | 305 | 383 | 2.22e-16 | 72.8 |
| MsG0480021236.01.T01 | MTR_0312s0030 | 39.024 | 82 | 39 | 4 | 11 | 84 | 245 | 323 | 6.83e-16 | 70.9 |
| MsG0480021236.01.T01 | MTR_1g007520 | 39.024 | 82 | 39 | 4 | 11 | 84 | 245 | 323 | 6.83e-16 | 70.9 |
| MsG0480021236.01.T01 | MTR_1g008590 | 39.024 | 82 | 39 | 4 | 11 | 84 | 245 | 323 | 7.86e-16 | 70.9 |
| MsG0480021236.01.T01 | MTR_1g008470 | 39.773 | 88 | 42 | 4 | 4 | 83 | 213 | 297 | 3.38e-15 | 68.9 |
| MsG0480021236.01.T01 | MTR_1g008510 | 36.585 | 82 | 41 | 4 | 11 | 84 | 246 | 324 | 7.84e-14 | 65.5 |
| MsG0480021236.01.T01 | MTR_1g008560 | 36.585 | 82 | 41 | 4 | 11 | 84 | 250 | 328 | 8.21e-14 | 65.1 |
| MsG0480021236.01.T01 | MTR_1g008460 | 38.636 | 88 | 43 | 4 | 4 | 83 | 210 | 294 | 1.12e-13 | 64.7 |
| MsG0480021236.01.T01 | MTR_1g008580 | 34.783 | 92 | 47 | 5 | 1 | 84 | 84 | 170 | 1.47e-13 | 62.8 |
| MsG0480021236.01.T01 | MTR_1g008530 | 34.783 | 92 | 47 | 5 | 1 | 84 | 136 | 222 | 3.27e-13 | 62.8 |
| MsG0480021236.01.T01 | MTR_8g445170 | 42.857 | 70 | 36 | 4 | 16 | 85 | 318 | 383 | 5.68e-11 | 57.4 |
| MsG0480021236.01.T01 | MTR_1g008450 | 37.037 | 81 | 40 | 4 | 11 | 83 | 233 | 310 | 9.64e-11 | 56.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021236.01.T01 | AT5G25610 | 43.210 | 81 | 35 | 4 | 11 | 83 | 312 | 389 | 1.26e-15 | 70.5 |
| MsG0480021236.01.T01 | AT1G49320 | 40.580 | 69 | 40 | 1 | 16 | 84 | 211 | 278 | 2.29e-15 | 69.3 |
Find 28 sgRNAs with CRISPR-Local
Find 27 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACCCAGATCATATTATATTT+AGG | 0.313828 | 4:-55598178 | MsG0480021236.01.T01:CDS |
| ATTTAGGCAGCTTGGCATTA+AGG | 0.314472 | 4:-55598162 | MsG0480021236.01.T01:CDS |
| TGCCTAAATATAATATGATC+TGG | 0.343870 | 4:+55598176 | None:intergenic |
| CTATGTAATGACAAAGTAAA+TGG | 0.375033 | 4:+55598300 | None:intergenic |
| TGATGCGAATGGAGATAAAA+TGG | 0.384644 | 4:-55598240 | MsG0480021236.01.T01:CDS |
| GCCATTTGGACACATCTGAT+TGG | 0.403046 | 4:-55598202 | MsG0480021236.01.T01:CDS |
| CTTTGTCATTACATAGCTAC+AGG | 0.415535 | 4:-55598293 | MsG0480021236.01.T01:CDS |
| AGCTGTTGGAATTTGCCATT+TGG | 0.418321 | 4:-55598216 | MsG0480021236.01.T01:CDS |
| ATGACAAAGTAAATGGCATA+TGG | 0.427641 | 4:+55598307 | None:intergenic |
| TACCTGTGAACCATCTTATG+TGG | 0.435229 | 4:-55598109 | MsG0480021236.01.T01:CDS |
| CATATTATATTTAGGCAGCT+TGG | 0.445595 | 4:-55598170 | MsG0480021236.01.T01:CDS |
| TACAGGGACTAGAGTGTTCA+AGG | 0.461314 | 4:-55598276 | MsG0480021236.01.T01:CDS |
| GCATTGGAACCCACATAAGA+TGG | 0.485432 | 4:+55598099 | None:intergenic |
| GTGTTCAAGGTCTCACTTGT+CGG | 0.486727 | 4:-55598263 | MsG0480021236.01.T01:CDS |
| TCCAATCAGATGTGTCCAAA+TGG | 0.503560 | 4:+55598201 | None:intergenic |
| AGGCAGCTTGGCATTAAGGC+CGG | 0.516232 | 4:-55598158 | MsG0480021236.01.T01:CDS |
| ACCTGTGAACCATCTTATGT+GGG | 0.517632 | 4:-55598108 | MsG0480021236.01.T01:CDS |
| GGAGATAAAATGGAAGCTGT+TGG | 0.517741 | 4:-55598230 | MsG0480021236.01.T01:CDS |
| GCCTAAATATAATATGATCT+GGG | 0.525219 | 4:+55598177 | None:intergenic |
| GGCAGCTTGGCATTAAGGCC+GGG | 0.541302 | 4:-55598157 | MsG0480021236.01.T01:CDS |
| ACACACTGGAGAGTTCTTCC+CGG | 0.545365 | 4:+55598139 | None:intergenic |
| TCACTTGTCGGTGATGCGAA+TGG | 0.570327 | 4:-55598251 | MsG0480021236.01.T01:CDS |
| ACCCACATAAGATGGTTCAC+AGG | 0.577363 | 4:+55598107 | None:intergenic |
| ATGGCATATGGATATGAGAG+TGG | 0.619183 | 4:+55598319 | None:intergenic |
| ACAGGTAAGAAGTGACACAC+TGG | 0.654861 | 4:+55598125 | None:intergenic |
| ATAGACGAACTCACTTCACA+TGG | 0.657262 | 4:+55598061 | None:intergenic |
| TTTGTCATTACATAGCTACA+GGG | 0.665648 | 4:-55598292 | MsG0480021236.01.T01:CDS |
| GACGAACTCACTTCACATGG+AGG | 0.694583 | 4:+55598064 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ACCCAGATCATATTATATTT+AGG | - | Chr4:55598233-55598252 | MsG0480021236.01.T01:CDS | 25.0% |
| ! | CTATGTAATGACAAAGTAAA+TGG | + | Chr4:55598114-55598133 | None:intergenic | 25.0% |
| ! | GCCTAAATATAATATGATCT+GGG | + | Chr4:55598237-55598256 | None:intergenic | 25.0% |
| ! | TGCCTAAATATAATATGATC+TGG | + | Chr4:55598238-55598257 | None:intergenic | 25.0% |
| ATGACAAAGTAAATGGCATA+TGG | + | Chr4:55598107-55598126 | None:intergenic | 30.0% | |
| CATATTATATTTAGGCAGCT+TGG | - | Chr4:55598241-55598260 | MsG0480021236.01.T01:CDS | 30.0% | |
| TTTGTCATTACATAGCTACA+GGG | - | Chr4:55598119-55598138 | MsG0480021236.01.T01:CDS | 30.0% | |
| CTTTGTCATTACATAGCTAC+AGG | - | Chr4:55598118-55598137 | MsG0480021236.01.T01:CDS | 35.0% | |
| TGATGCGAATGGAGATAAAA+TGG | - | Chr4:55598171-55598190 | MsG0480021236.01.T01:CDS | 35.0% | |
| ACCTGTGAACCATCTTATGT+GGG | - | Chr4:55598303-55598322 | MsG0480021236.01.T01:CDS | 40.0% | |
| AGCTGTTGGAATTTGCCATT+TGG | - | Chr4:55598195-55598214 | MsG0480021236.01.T01:CDS | 40.0% | |
| ATGGCATATGGATATGAGAG+TGG | + | Chr4:55598095-55598114 | None:intergenic | 40.0% | |
| ATTTAGGCAGCTTGGCATTA+AGG | - | Chr4:55598249-55598268 | MsG0480021236.01.T01:CDS | 40.0% | |
| GGAGATAAAATGGAAGCTGT+TGG | - | Chr4:55598181-55598200 | MsG0480021236.01.T01:CDS | 40.0% | |
| TACCTGTGAACCATCTTATG+TGG | - | Chr4:55598302-55598321 | MsG0480021236.01.T01:CDS | 40.0% | |
| TCCAATCAGATGTGTCCAAA+TGG | + | Chr4:55598213-55598232 | None:intergenic | 40.0% | |
| ACAGGTAAGAAGTGACACAC+TGG | + | Chr4:55598289-55598308 | None:intergenic | 45.0% | |
| ACCCACATAAGATGGTTCAC+AGG | + | Chr4:55598307-55598326 | None:intergenic | 45.0% | |
| GCATTGGAACCCACATAAGA+TGG | + | Chr4:55598315-55598334 | None:intergenic | 45.0% | |
| GCCATTTGGACACATCTGAT+TGG | - | Chr4:55598209-55598228 | MsG0480021236.01.T01:CDS | 45.0% | |
| GTGTTCAAGGTCTCACTTGT+CGG | - | Chr4:55598148-55598167 | MsG0480021236.01.T01:CDS | 45.0% | |
| ! | TACAGGGACTAGAGTGTTCA+AGG | - | Chr4:55598135-55598154 | MsG0480021236.01.T01:CDS | 45.0% |
| !! | GAGGCTTTTGAAGGTTGCAT+TGG | + | Chr4:55598331-55598350 | None:intergenic | 45.0% |
| ACACACTGGAGAGTTCTTCC+CGG | + | Chr4:55598275-55598294 | None:intergenic | 50.0% | |
| TCACTTGTCGGTGATGCGAA+TGG | - | Chr4:55598160-55598179 | MsG0480021236.01.T01:CDS | 50.0% | |
| AGGCAGCTTGGCATTAAGGC+CGG | - | Chr4:55598253-55598272 | MsG0480021236.01.T01:CDS | 55.0% | |
| GGCAGCTTGGCATTAAGGCC+GGG | - | Chr4:55598254-55598273 | MsG0480021236.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 55598076 | 55598357 | 55598076 | ID=MsG0480021236.01;Name=MsG0480021236.01 |
| Chr4 | mRNA | 55598076 | 55598357 | 55598076 | ID=MsG0480021236.01.T01;Parent=MsG0480021236.01;Name=MsG0480021236.01.T01;_AED=0.48;_eAED=0.48;_QI=0|-1|0|1|-1|1|1|0|93 |
| Chr4 | exon | 55598076 | 55598357 | 55598076 | ID=MsG0480021236.01.T01:exon:6302;Parent=MsG0480021236.01.T01 |
| Chr4 | CDS | 55598076 | 55598357 | 55598076 | ID=MsG0480021236.01.T01:cds;Parent=MsG0480021236.01.T01 |
| Gene Sequence |
| Protein sequence |
