AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280010888.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280010888.01.T03 MTR_2g093770 100.000 48 0 0 1 48 60 107 1.25e-30 102
MsG0280010888.01.T03 MTR_1g082400 91.111 45 4 0 1 45 61 105 2.51e-24 87.0
MsG0280010888.01.T03 MTR_4g028080 75.000 44 11 0 1 44 37 80 1.07e-17 68.9
MsG0280010888.01.T03 MTR_8g086330 76.744 43 10 0 1 43 37 79 1.18e-17 68.9
MsG0280010888.01.T02 MTR_2g093770 97.778 45 1 0 1 45 1 45 1.22e-14 62.8
MsG0280010888.01.T01 MTR_2g093770 100.000 63 0 0 20 82 45 107 3.98e-41 130
MsG0280010888.01.T01 MTR_1g082400 89.362 47 5 0 33 79 59 105 1.12e-25 91.7
MsG0280010888.01.T01 MTR_8g086330 57.576 66 28 0 12 77 14 79 7.19e-19 73.6
MsG0280010888.01.T01 MTR_4g028080 58.209 67 28 0 12 78 14 80 9.84e-19 73.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280010888.01.T03 AT5G60460 89.130 46 5 0 1 46 62 107 3.17e-25 89.0
MsG0280010888.01.T03 AT5G60460 89.130 46 5 0 1 46 62 107 3.17e-25 89.0
MsG0280010888.01.T03 AT3G60540 72.727 44 12 0 1 44 37 80 2.42e-17 68.2
MsG0280010888.01.T03 AT3G60540 72.727 44 12 0 1 44 37 80 2.42e-17 68.2
MsG0280010888.01.T03 AT3G60540 72.727 44 12 0 1 44 37 80 2.42e-17 68.2
MsG0280010888.01.T03 AT2G45070 77.500 40 9 0 1 40 40 79 3.33e-17 67.8
MsG0280010888.01.T03 AT2G45070 77.500 40 9 0 1 40 40 79 3.33e-17 67.8
MsG0280010888.01.T03 AT2G45070 77.500 40 9 0 1 40 40 79 3.33e-17 67.8
MsG0280010888.01.T03 AT2G45070 77.500 40 9 0 1 40 40 79 3.33e-17 67.8
MsG0280010888.01.T01 AT5G60460 89.362 47 5 0 34 80 61 107 3.35e-26 93.2
MsG0280010888.01.T01 AT5G60460 89.362 47 5 0 34 80 61 107 3.35e-26 93.2
MsG0280010888.01.T01 AT3G60540 51.389 72 35 0 7 78 9 80 5.05e-18 71.6
MsG0280010888.01.T01 AT3G60540 51.389 72 35 0 7 78 9 80 5.05e-18 71.6
MsG0280010888.01.T01 AT3G60540 51.389 72 35 0 7 78 9 80 5.05e-18 71.6
MsG0280010888.01.T01 AT2G45070 74.419 43 11 0 35 77 40 82 1.13e-17 70.5
MsG0280010888.01.T01 AT2G45070 74.419 43 11 0 35 77 40 82 1.13e-17 70.5
MsG0280010888.01.T01 AT2G45070 74.419 43 11 0 35 77 40 82 1.13e-17 70.5
MsG0280010888.01.T01 AT2G45070 74.419 43 11 0 35 77 40 82 1.13e-17 70.5

Find 41 sgRNAs with CRISPR-Local

Find 40 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAACGCTGGCGGTGCTGTTC+CGG 0.259648 2:-76426819 None:intergenic
GATTTCACCGACGGTGGTTT+TGG 0.294926 2:+76426906 MsG0280010888.01.T01:CDS
GTCACCGCGCTTCATGTGTT+CGG 0.324439 2:+76426955 MsG0280010888.01.T01:CDS
TCACCGCGCTTCATGTGTTC+GGG 0.377638 2:+76426956 MsG0280010888.01.T01:CDS
TCCTCCTGGACGGGAGGATG+TGG 0.389528 2:-76426729 None:intergenic
GGCTCCGCTGCCGCCACAGC+CGG 0.408056 2:+76426773 MsG0280010888.01.T01:CDS
CATGCCGGCTGTGGCGGCAG+CGG 0.430788 2:-76426777 None:intergenic
GCAGCGAGTCCGCCTCCTCC+TGG 0.437060 2:-76426743 None:intergenic
TCCTCCCGTCCAGGAGGAGG+CGG 0.463865 2:+76426734 MsG0280010888.01.T02:CDS
AACAGCACCGCCAGCGTTGG+AGG 0.473828 2:+76426823 MsG0280010888.01.T01:CDS
ACATCCTCCCGTCCAGGAGG+AGG 0.475747 2:+76426731 MsG0280010888.01.T02:CDS
TATCGTTACAAAGCTGGTGC+TGG 0.477938 2:+76426985 MsG0280010888.01.T01:CDS
ACCTCCACATCCTCCCGTCC+AGG 0.490070 2:+76426725 MsG0280010888.01.T02:CDS
AAGCTTTATCGTTACAAAGC+TGG 0.494177 2:+76426979 MsG0280010888.01.T01:CDS
AGCACCGCCAGCGTTGGAGG+TGG 0.498786 2:+76426826 MsG0280010888.01.T01:CDS
TCGACGACGCATGCCGGCTG+TGG 0.506267 2:-76426786 None:intergenic
ACGACGCATGCCGGCTGTGG+CGG 0.508943 2:-76426783 None:intergenic
GCTAGATCCACCTCCAACGC+TGG 0.511643 2:-76426833 None:intergenic
ATTCTACACTGACGATGCAC+CGG 0.515056 2:+76426876 MsG0280010888.01.T01:CDS
CGTCGTCGACGTCTCACCTC+CGG 0.515826 2:+76426800 MsG0280010888.01.T01:CDS
CGCTGGCGGTGCTGTTCCGG+AGG 0.517315 2:-76426816 None:intergenic
TTCTACACTGACGATGCACC+GGG 0.517664 2:+76426877 MsG0280010888.01.T01:CDS
CGGAACAGCACCGCCAGCGT+TGG 0.521629 2:+76426820 MsG0280010888.01.T01:CDS
CGAGTCCGCCTCCTCCTGGA+CGG 0.525894 2:-76426739 None:intergenic
TCCTGGACGGGAGGATGTGG+AGG 0.527989 2:-76426726 None:intergenic
GAGTCCGCCTCCTCCTGGAC+GGG 0.535624 2:-76426738 None:intergenic
TCCGCCTCCTCCTGGACGGG+AGG 0.535956 2:-76426735 None:intergenic
GTTATGAGTCTCTGCTTCAT+CGG 0.539263 2:+76426928 MsG0280010888.01.T01:CDS
TCTCCACTCATCATGGCTAA+AGG 0.547275 2:+76426680 None:intergenic
TTTGAAGATTTCACCGACGG+TGG 0.598789 2:+76426900 MsG0280010888.01.T01:CDS
GAGACGTCGACGACGCATGC+CGG 0.602996 2:-76426792 None:intergenic
GTGGCGGCAGCGGAGCCACG+TGG 0.617814 2:-76426767 None:intergenic
CTCATAACCAAAACCACCGT+CGG 0.617981 2:-76426913 None:intergenic
AGATCCACCTCCAACGCTGG+CGG 0.627371 2:-76426830 None:intergenic
AGCGTTGGAGGTGGATCTAG+CGG 0.636568 2:+76426835 MsG0280010888.01.T01:CDS
GTCGGTGAAATCTTCAAACC+CGG 0.639473 2:-76426895 None:intergenic
GGGTTTGAAGATTTCACCGA+CGG 0.641586 2:+76426897 MsG0280010888.01.T01:CDS
GGCGGACTCGCTGCACCACG+TGG 0.650379 2:+76426752 MsG0280010888.01.T02:CDS
TCCACATCCTCCCGTCCAGG+AGG 0.666674 2:+76426728 MsG0280010888.01.T02:CDS
GTTGGAGGTGGATCTAGCGG+AGG 0.684640 2:+76426838 MsG0280010888.01.T01:CDS
CTTCCCGAACACATGAAGCG+CGG 0.732527 2:-76426959 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAGCTTTATCGTTACAAAGC+TGG + Chr2:76426979-76426998 MsG0280010888.01.T01:CDS 35.0%
GTTATGAGTCTCTGCTTCAT+CGG + Chr2:76426928-76426947 MsG0280010888.01.T01:CDS 40.0%
ATTCTACACTGACGATGCAC+CGG + Chr2:76426876-76426895 MsG0280010888.01.T01:CDS 45.0%
CTCATAACCAAAACCACCGT+CGG - Chr2:76426916-76426935 None:intergenic 45.0%
GGGTTTGAAGATTTCACCGA+CGG + Chr2:76426897-76426916 MsG0280010888.01.T01:CDS 45.0%
GTCGGTGAAATCTTCAAACC+CGG - Chr2:76426898-76426917 None:intergenic 45.0%
TATCGTTACAAAGCTGGTGC+TGG + Chr2:76426985-76427004 MsG0280010888.01.T01:CDS 45.0%
TTTGAAGATTTCACCGACGG+TGG + Chr2:76426900-76426919 MsG0280010888.01.T01:CDS 45.0%
TTCTACACTGACGATGCACC+GGG + Chr2:76426877-76426896 MsG0280010888.01.T01:CDS 50.0%
! GATTTCACCGACGGTGGTTT+TGG + Chr2:76426906-76426925 MsG0280010888.01.T01:CDS 50.0%
CTTCCCGAACACATGAAGCG+CGG - Chr2:76426962-76426981 None:intergenic 55.0%
GTCACCGCGCTTCATGTGTT+CGG + Chr2:76426955-76426974 MsG0280010888.01.T01:CDS 55.0%
TCACCGCGCTTCATGTGTTC+GGG + Chr2:76426956-76426975 MsG0280010888.01.T01:CDS 55.0%
! AGCGTTGGAGGTGGATCTAG+CGG + Chr2:76426835-76426854 MsG0280010888.01.T01:CDS 55.0%
AGATCCACCTCCAACGCTGG+CGG - Chr2:76426833-76426852 None:intergenic 60.0%
GCTAGATCCACCTCCAACGC+TGG - Chr2:76426836-76426855 None:intergenic 60.0%
GTTGGAGGTGGATCTAGCGG+AGG + Chr2:76426838-76426857 MsG0280010888.01.T01:CDS 60.0%
ACATCCTCCCGTCCAGGAGG+AGG + Chr2:76426731-76426750 MsG0280010888.01.T02:CDS 65.0%
ACCTCCACATCCTCCCGTCC+AGG + Chr2:76426725-76426744 MsG0280010888.01.T02:CDS 65.0%
CGTCGTCGACGTCTCACCTC+CGG + Chr2:76426800-76426819 MsG0280010888.01.T01:CDS 65.0%
GAGACGTCGACGACGCATGC+CGG - Chr2:76426795-76426814 None:intergenic 65.0%
TCCACATCCTCCCGTCCAGG+AGG + Chr2:76426728-76426747 MsG0280010888.01.T02:CDS 65.0%
TCCTCCTGGACGGGAGGATG+TGG - Chr2:76426732-76426751 None:intergenic 65.0%
TCCTGGACGGGAGGATGTGG+AGG - Chr2:76426729-76426748 None:intergenic 65.0%
! AACAGCACCGCCAGCGTTGG+AGG + Chr2:76426823-76426842 MsG0280010888.01.T01:CDS 65.0%
!! CAACGCTGGCGGTGCTGTTC+CGG - Chr2:76426822-76426841 None:intergenic 65.0%
CGAGTCCGCCTCCTCCTGGA+CGG - Chr2:76426742-76426761 None:intergenic 70.0%
CGGAACAGCACCGCCAGCGT+TGG + Chr2:76426820-76426839 MsG0280010888.01.T01:CDS 70.0%
GAGTCCGCCTCCTCCTGGAC+GGG - Chr2:76426741-76426760 None:intergenic 70.0%
TCCTCCCGTCCAGGAGGAGG+CGG + Chr2:76426734-76426753 MsG0280010888.01.T02:CDS 70.0%
! ACGACGCATGCCGGCTGTGG+CGG - Chr2:76426786-76426805 None:intergenic 70.0%
! AGCACCGCCAGCGTTGGAGG+TGG + Chr2:76426826-76426845 MsG0280010888.01.T01:CDS 70.0%
! TCGACGACGCATGCCGGCTG+TGG - Chr2:76426789-76426808 None:intergenic 70.0%
GCAGCGAGTCCGCCTCCTCC+TGG - Chr2:76426746-76426765 None:intergenic 75.0%
TCCGCCTCCTCCTGGACGGG+AGG - Chr2:76426738-76426757 None:intergenic 75.0%
! CATGCCGGCTGTGGCGGCAG+CGG - Chr2:76426780-76426799 None:intergenic 75.0%
! GGCGGACTCGCTGCACCACG+TGG + Chr2:76426752-76426771 MsG0280010888.01.T02:CDS 75.0%
!! CGCTGGCGGTGCTGTTCCGG+AGG - Chr2:76426819-76426838 None:intergenic 75.0%
! GGCTCCGCTGCCGCCACAGC+CGG + Chr2:76426773-76426792 MsG0280010888.01.T01:CDS 80.0%
! GTGGCGGCAGCGGAGCCACG+TGG - Chr2:76426770-76426789 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
Chr2 gene 76426692 76427014 76426692 ID=MsG0280010888.01;Name=MsG0280010888.01
Chr2 mRNA 76426766 76427014 76426766 ID=MsG0280010888.01.T01;Parent=MsG0280010888.01;Name=MsG0280010888.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|82
Chr2 exon 76426766 76427014 76426766 ID=MsG0280010888.01.T01:exon:11873;Parent=MsG0280010888.01.T01
Chr2 CDS 76426766 76427014 76426766 ID=MsG0280010888.01.T01:cds;Parent=MsG0280010888.01.T01
Chr2 mRNA 76426692 76426874 76426692 ID=MsG0280010888.01.T02;Parent=MsG0280010888.01;Name=MsG0280010888.01.T02;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|60
Chr2 exon 76426692 76426874 76426692 ID=MsG0280010888.01.T02:exon:11874;Parent=MsG0280010888.01.T02
Chr2 CDS 76426692 76426874 76426692 ID=MsG0280010888.01.T02:cds;Parent=MsG0280010888.01.T02
Chr2 mRNA 76426868 76427014 76426868 ID=MsG0280010888.01.T03;Parent=MsG0280010888.01;Name=MsG0280010888.01.T03;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|48
Chr2 exon 76426868 76427014 76426868 ID=MsG0280010888.01.T03:exon:11875;Parent=MsG0280010888.01.T03
Chr2 CDS 76426868 76427014 76426868 ID=MsG0280010888.01.T03:cds;Parent=MsG0280010888.01.T03
Gene Sequence

>MsG0280010888.01.T03

ATGCTGAGATTCTACACTGACGATGCACCGGGTTTGAAGATTTCACCGACGGTGGTTTTGGTTATGAGTCTCTGCTTCATCGGTTTCGTCACCGCGCTTCATGTGTTCGGGAAGCTTTATCGTTACAAAGCTGGTGCTGGTGTATAA

>MsG0280010888.01.T02

ATGGCTAAAGGCGCATCTCAATCTCAATCATCAACCTCCACATCCTCCCGTCCAGGAGGAGGCGGACTCGCTGCACCACGTGGCTCCGCTGCCGCCACAGCCGGCATGCGTCGTCGACGTCTCACCTCCGGAACAGCACCGCCAGCGTTGGAGGTGGATCTAGCGGAGGAAGCAACATGCTGA

>MsG0280010888.01.T01

ACCACGTGGCTCCGCTGCCGCCACAGCCGGCATGCGTCGTCGACGTCTCACCTCCGGAACAGCACCGCCAGCGTTGGAGGTGGATCTAGCGGAGGAAGCAACATGCTGAGATTCTACACTGACGATGCACCGGGTTTGAAGATTTCACCGACGGTGGTTTTGGTTATGAGTCTCTGCTTCATCGGTTTCGTCACCGCGCTTCATGTGTTCGGGAAGCTTTATCGTTACAAAGCTGGTGCTGGTGTATAA

Protein sequence

>MsG0280010888.01.T03

MLRFYTDDAPGLKISPTVVLVMSLCFIGFVTALHVFGKLYRYKAGAGV*

>MsG0280010888.01.T02

MAKGASQSQSSTSTSSRPGGGGLAAPRGSAAATAGMRRRRLTSGTAPPALEVDLAEEATC*

>MsG0280010888.01.T01

TTWLRCRHSRHASSTSHLRNSTASVGGGSSGGSNMLRFYTDDAPGLKISPTVVLVMSLCFIGFVTALHVFGKLYRYKAGAGV*