AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480022968.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022968.01.T01 MTR_8g080750 92.500 80 6 0 8 87 144 223 4.82e-47 152
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022968.01.T01 AT1G27330 88.235 68 8 0 45 112 1 68 4.64e-38 123
MsG0480022968.01.T01 AT1G27350 88.235 68 8 0 45 112 1 68 4.64e-38 123

Find 30 sgRNAs with CRISPR-Local

Find 107 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTTTCTTAACAGTTGTTTC+TGG 0.223249 4:+79269530 None:intergenic
ATGATTGATTTGTAGGATAA+TGG 0.270299 4:+79270023 None:intergenic
TTCTTCGTCTTTGTTGTCAT+TGG 0.272950 4:-79269473 MsG0480022968.01.T01:CDS
ACGAACCATGATTGATTTGT+AGG 0.306992 4:+79270016 None:intergenic
ACGAAGAAACCAAGCAAAAC+GGG 0.342450 4:+79269488 None:intergenic
CAGCTCTCTTTCAGATAATC+AGG 0.365308 4:-79268543 MsG0480022968.01.T01:CDS
CCAAGCAAAACGGGGCCAAC+AGG 0.391773 4:+79269497 None:intergenic
AACTTCATCAAAACATGAAC+AGG 0.410889 4:+79268404 None:intergenic
GACGAAGAAACCAAGCAAAA+CGG 0.413008 4:+79269487 None:intergenic
CATCAAAGCGCCTTGCAGTT+AGG 0.424154 4:-79269598 MsG0480022968.01.T01:CDS
ATCAAAACATGAACAGGAAC+TGG 0.442632 4:+79268410 None:intergenic
GGCATAATGGAGCTTTGAGT+TGG 0.445961 4:-79268500 MsG0480022968.01.T01:exon
CGAGTGGAGGCATGGCATAA+TGG 0.448620 4:-79268513 MsG0480022968.01.T01:exon
TATGTAAAATTAAACAAAAG+AGG 0.501977 4:+79268344 None:intergenic
CCAGAAACAACTGTTAAGAA+AGG 0.524644 4:-79269530 MsG0480022968.01.T01:CDS
ATTATCCTACAAATCAATCA+TGG 0.554540 4:-79270021 MsG0480022968.01.T01:intron
AAAGGAAATGACTATCCTGT+TGG 0.571369 4:-79269512 MsG0480022968.01.T01:CDS
GACAGCAACGAGTGGAGGCA+TGG 0.571659 4:-79268521 MsG0480022968.01.T01:CDS
AAAGCGCCTTGCAGTTAGGA+AGG 0.575086 4:-79269594 MsG0480022968.01.T01:CDS
TTTGTAGGATAATGGATCGC+CGG 0.595314 4:+79270031 None:intergenic
ACCTCGGAGAGAGTCACTGC+CGG 0.598531 4:-79270107 MsG0480022968.01.T01:CDS
ATCAGGACAGCAACGAGTGG+AGG 0.608196 4:-79268526 MsG0480022968.01.T01:CDS
CGAAGAAACCAAGCAAAACG+GGG 0.617394 4:+79269489 None:intergenic
TCCGGCAGTGACTCTCTCCG+AGG 0.626564 4:+79270106 None:intergenic
GAAATCTCTAAATAAAACCT+CGG 0.627274 4:-79270123 MsG0480022968.01.T01:CDS
TCTCAACCTTCCTAACTGCA+AGG 0.647474 4:+79269588 None:intergenic
ATAATCAGGACAGCAACGAG+TGG 0.658965 4:-79268529 MsG0480022968.01.T01:CDS
GTAGGATAATGGATCGCCGG+TGG 0.665340 4:+79270034 None:intergenic
AACGCCGGAAAATTGAAAGA+CGG 0.693395 4:+79270069 None:intergenic
GCCGGAAAATTGAAAGACGG+TGG 0.699767 4:+79270072 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAATTAGTAAAACACATA+AGG + Chr4:79269076-79269095 None:intergenic 15.0%
!! AAAAATTTACTATATTACAG+AGG + Chr4:79269370-79269389 None:intergenic 15.0%
!! AAAATTTACTATATTACAGA+GGG + Chr4:79269369-79269388 None:intergenic 15.0%
!! AATCAGAAATTTATCAAATT+AGG - Chr4:79268685-79268704 MsG0480022968.01.T01:intron 15.0%
!! CAATATTTCAAAAATTGAAT+CGG + Chr4:79269325-79269344 None:intergenic 15.0%
!! TCAATTTCAAAACAAAAATT+CGG + Chr4:79268623-79268642 None:intergenic 15.0%
!! TTGTTAAATTTGATTGAATT+TGG - Chr4:79268806-79268825 MsG0480022968.01.T01:intron 15.0%
!!! AATATTTTCAATTCAATTCA+AGG + Chr4:79269617-79269636 None:intergenic 15.0%
!!! AATTTTTGAAATATTGTTGT+GGG - Chr4:79269329-79269348 MsG0480022968.01.T01:intron 15.0%
!! ACTATATTATGTGTTGAAAT+TGG + Chr4:79269537-79269556 None:intergenic 20.0%
!! AGGGTAATAAAAAAAATTAC+AGG + Chr4:79269413-79269432 None:intergenic 20.0%
!! ATCAAAACAAAAAATTGTAC+AGG + Chr4:79269433-79269452 None:intergenic 20.0%
!! GGGTAATAAAAAAAATTACA+GGG + Chr4:79269412-79269431 None:intergenic 20.0%
!! GGTAATAAAAAAAATTACAG+GGG + Chr4:79269411-79269430 None:intergenic 20.0%
!! TATTGTTTGTGTGATTTATT+TGG - Chr4:79269145-79269164 MsG0480022968.01.T01:intron 20.0%
!! TCAAAACAAAAAATTGTACA+GGG + Chr4:79269432-79269451 None:intergenic 20.0%
!!! ATTTTTGAAATATTGTTGTG+GGG - Chr4:79269330-79269349 MsG0480022968.01.T01:intron 20.0%
!!! CAAGAAATTTTGTTTTTGTT+TGG + Chr4:79269471-79269490 None:intergenic 20.0%
!!! CAATTTTTGAAATATTGTTG+TGG - Chr4:79269328-79269347 MsG0480022968.01.T01:intron 20.0%
! AATTAGTAAAACACATAAGG+AGG + Chr4:79269073-79269092 None:intergenic 25.0%
! ATGTTGAATGAAATGAGATT+TGG - Chr4:79268836-79268855 MsG0480022968.01.T01:intron 25.0%
! ATTATCCTACAAATCAATCA+TGG - Chr4:79268470-79268489 MsG0480022968.01.T01:three_prime_UTR 25.0%
! CTGAGTACATAATTTATTCT+CGG - Chr4:79269591-79269610 MsG0480022968.01.T01:CDS 25.0%
! GAAATCTCTAAATAAAACCT+CGG - Chr4:79268368-79268387 MsG0480022968.01.T01:three_prime_UTR 25.0%
! GAGAAAAATATCACAAAAAG+AGG - Chr4:79268931-79268950 MsG0480022968.01.T01:intron 25.0%
! GTATATCAAACATTTGATCA+CGG + Chr4:79269799-79269818 None:intergenic 25.0%
! TATATCAAACATTTGATCAC+GGG + Chr4:79269798-79269817 None:intergenic 25.0%
!! AGTATAGTCATATTTTCTAC+AGG + Chr4:79270039-79270058 None:intergenic 25.0%
!! GTATAGTCATATTTTCTACA+GGG + Chr4:79270038-79270057 None:intergenic 25.0%
!! GTCAAATTTTATGTAGCTTA+TGG - Chr4:79268707-79268726 MsG0480022968.01.T01:intron 25.0%
!!! ATGATTGATTTGTAGGATAA+TGG + Chr4:79268471-79268490 None:intergenic 25.0%
!!! CATGTTTTGATGAAGTTTTA+AGG - Chr4:79270093-79270112 MsG0480022968.01.T01:CDS 25.0%
!!! TGAAATGAGATTTGGAATTT+TGG - Chr4:79268844-79268863 MsG0480022968.01.T01:intron 25.0%
!!! TTACATTGATTTTCAGCTTA+TGG - Chr4:79268741-79268760 MsG0480022968.01.T01:intron 25.0%
AAATGTTTGATATACCTCTC+AGG - Chr4:79269804-79269823 MsG0480022968.01.T01:intron 30.0%
AACTTCATCAAAACATGAAC+AGG + Chr4:79270090-79270109 None:intergenic 30.0%
AATTAACAATAGTTCAGCTC+TGG + Chr4:79269226-79269245 None:intergenic 30.0%
ACTGAACCGATAGTTAAAAT+CGG - Chr4:79269195-79269214 MsG0480022968.01.T01:intron 30.0%
CCTAATTCTTGTGATGAATT+GGG - Chr4:79269116-79269135 MsG0480022968.01.T01:intron 30.0%
CGAGAATAAATTATGTACTC+AGG + Chr4:79269593-79269612 None:intergenic 30.0%
GAGCTGAACTATTGTTAATT+CGG - Chr4:79269226-79269245 MsG0480022968.01.T01:intron 30.0%
GTAGAATCATGACAAAAACA+AGG + Chr4:79268579-79268598 None:intergenic 30.0%
TCCTAATTCTTGTGATGAAT+TGG - Chr4:79269115-79269134 MsG0480022968.01.T01:intron 30.0%
TGTTAATTCGGATATTTGAG+AGG - Chr4:79269238-79269257 MsG0480022968.01.T01:intron 30.0%
TTAACAATTCCCTATGGAAT+TGG - Chr4:79269770-79269789 MsG0480022968.01.T01:intron 30.0%
! CTATACTTTTCTACATGTAC+TGG - Chr4:79270052-79270071 MsG0480022968.01.T01:CDS 30.0%
!! AATGAGATTTGGAATTTTGG+TGG - Chr4:79268847-79268866 MsG0480022968.01.T01:intron 30.0%
!! TGTTTTTGTTTGGCCTATTA+GGG + Chr4:79269461-79269480 None:intergenic 30.0%
!!! TTGTTTTTGTTTGGCCTATT+AGG + Chr4:79269462-79269481 None:intergenic 30.0%
AAAGGAAATGACTATCCTGT+TGG - Chr4:79268979-79268998 MsG0480022968.01.T01:intron 35.0%
AATCGTATTATAGTGCAGAG+AGG - Chr4:79269717-79269736 MsG0480022968.01.T01:intron 35.0%
AGGGATTTAACAATTCCCTA+TGG - Chr4:79269764-79269783 MsG0480022968.01.T01:intron 35.0%
ATAAGGAGGACAACATTGAA+AGG + Chr4:79269059-79269078 None:intergenic 35.0%
ATCAAAACATGAACAGGAAC+TGG + Chr4:79270084-79270103 None:intergenic 35.0%
ATCGTATTATAGTGCAGAGA+GGG - Chr4:79269718-79269737 MsG0480022968.01.T01:intron 35.0%
CCAGAAACAACTGTTAAGAA+AGG - Chr4:79268961-79268980 MsG0480022968.01.T01:intron 35.0%
CCCAATTCATCACAAGAATT+AGG + Chr4:79269119-79269138 None:intergenic 35.0%
GAATGCAAGAAATAGAAGAG+AGG + Chr4:79268507-79268526 None:intergenic 35.0%
TAAGGAGGACAACATTGAAA+GGG + Chr4:79269058-79269077 None:intergenic 35.0%
TATATCATCCTAATCCTGAG+AGG + Chr4:79269821-79269840 None:intergenic 35.0%
TCAAGCTGTTGAACTATTTG+AGG - Chr4:79269282-79269301 MsG0480022968.01.T01:intron 35.0%
TTGATATACCTCTCAGGATT+AGG - Chr4:79269810-79269829 MsG0480022968.01.T01:intron 35.0%
! CCTTTCTTAACAGTTGTTTC+TGG + Chr4:79268964-79268983 None:intergenic 35.0%
! TTCTTCGTCTTTGTTGTCAT+TGG - Chr4:79269018-79269037 MsG0480022968.01.T01:intron 35.0%
! TTGAACTATTTGAGGTCTTG+TGG - Chr4:79269290-79269309 MsG0480022968.01.T01:intron 35.0%
!! ACGAACCATGATTGATTTGT+AGG + Chr4:79268478-79268497 None:intergenic 35.0%
!! AGGTTCAATCGTTGTTTTGT+TGG - Chr4:79269258-79269277 MsG0480022968.01.T01:intron 35.0%
!! GTTTTTGTTTGGCCTATTAG+GGG + Chr4:79269460-79269479 None:intergenic 35.0%
!! TTTTTGTTTGGCCTATTAGG+GGG + Chr4:79269459-79269478 None:intergenic 35.0%
!!! GCTCAACCGATTTTAACTAT+CGG + Chr4:79269204-79269223 None:intergenic 35.0%
!!! GTTTTGATTTACCCCCTAAT+AGG - Chr4:79269445-79269464 MsG0480022968.01.T01:intron 35.0%
AACGCCGGAAAATTGAAAGA+CGG + Chr4:79268425-79268444 None:intergenic 40.0%
ACATAGCTCATGACATGCTA+TGG + Chr4:79269855-79269874 None:intergenic 40.0%
ACGAAGAAACCAAGCAAAAC+GGG + Chr4:79269006-79269025 None:intergenic 40.0%
ATTATAGTGCAGAGAGGGAA+AGG - Chr4:79269723-79269742 MsG0480022968.01.T01:intron 40.0%
CAGCTCTCTTTCAGATAATC+AGG - Chr4:79269948-79269967 MsG0480022968.01.T01:intron 40.0%
CATGACATGCTATGGAAGAA+AGG + Chr4:79269847-79269866 None:intergenic 40.0%
GACGAAGAAACCAAGCAAAA+CGG + Chr4:79269007-79269026 None:intergenic 40.0%
TTATAGTGCAGAGAGGGAAA+GGG - Chr4:79269724-79269743 MsG0480022968.01.T01:intron 40.0%
! ACCACCGTCTTTCAATTTTC+CGG - Chr4:79268418-79268437 MsG0480022968.01.T01:three_prime_UTR 40.0%
!! GGTAATGGGTAATGGTCAAA+GGG - Chr4:79269745-79269764 MsG0480022968.01.T01:intron 40.0%
!! TTTGTAGGATAATGGATCGC+CGG + Chr4:79268463-79268482 None:intergenic 40.0%
ATAATCAGGACAGCAACGAG+TGG - Chr4:79269962-79269981 MsG0480022968.01.T01:intron 45.0%
CGAAGAAACCAAGCAAAACG+GGG + Chr4:79269005-79269024 None:intergenic 45.0%
TCTCAACCTTCCTAACTGCA+AGG + Chr4:79268906-79268925 None:intergenic 45.0%
TGGAAAAACGAAGAGAACGC+CGG + Chr4:79268440-79268459 None:intergenic 45.0%
!! ACGGTGGTGATGTTCTTTTC+CGG + Chr4:79268406-79268425 None:intergenic 45.0%
!! AGGGAAAGGGTAATGGGTAA+TGG - Chr4:79269737-79269756 MsG0480022968.01.T01:intron 45.0%
!! CGTTCTCTTCGTTTTTCCAC+CGG - Chr4:79268441-79268460 MsG0480022968.01.T01:three_prime_UTR 45.0%
!! GGCATAATGGAGCTTTGAGT+TGG - Chr4:79269991-79270010 MsG0480022968.01.T01:intron 45.0%
!! GGGTAATGGGTAATGGTCAA+AGG - Chr4:79269744-79269763 MsG0480022968.01.T01:intron 45.0%
!!! TAAATTTTTGAAATATTTTA+TGG - Chr4:79269381-79269400 MsG0480022968.01.T01:intron 5.0%
ATCACGGGCACCAATTCCAT+AGG + Chr4:79269783-79269802 None:intergenic 50.0%
GCAGAGAGGGAAAGGGTAAT+GGG - Chr4:79269731-79269750 MsG0480022968.01.T01:intron 50.0%
GCCGGAAAATTGAAAGACGG+TGG + Chr4:79268422-79268441 None:intergenic 50.0%
TCACGGGCACCAATTCCATA+GGG + Chr4:79269782-79269801 None:intergenic 50.0%
TGCAGAGAGGGAAAGGGTAA+TGG - Chr4:79269730-79269749 MsG0480022968.01.T01:intron 50.0%
! AAAGCGCCTTGCAGTTAGGA+AGG - Chr4:79268897-79268916 MsG0480022968.01.T01:intron 50.0%
! CATCAAAGCGCCTTGCAGTT+AGG - Chr4:79268893-79268912 MsG0480022968.01.T01:intron 50.0%
ATCAGGACAGCAACGAGTGG+AGG - Chr4:79269965-79269984 MsG0480022968.01.T01:intron 55.0%
! CGAGTGGAGGCATGGCATAA+TGG - Chr4:79269978-79269997 MsG0480022968.01.T01:intron 55.0%
!! GTAGGATAATGGATCGCCGG+TGG + Chr4:79268460-79268479 None:intergenic 55.0%
ACCTCGGAGAGAGTCACTGC+CGG - Chr4:79268384-79268403 MsG0480022968.01.T01:three_prime_UTR 60.0%
CCAAGCAAAACGGGGCCAAC+AGG + Chr4:79268997-79269016 None:intergenic 60.0%
GACAGCAACGAGTGGAGGCA+TGG - Chr4:79269970-79269989 MsG0480022968.01.T01:intron 60.0%
! CCTGTTGGCCCCGTTTTGCT+TGG - Chr4:79268994-79269013 MsG0480022968.01.T01:intron 60.0%
TCCGGCAGTGACTCTCTCCG+AGG + Chr4:79268388-79268407 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr4 gene 79268357 79270156 79268357 ID=MsG0480022968.01;Name=MsG0480022968.01
Chr4 mRNA 79268357 79270156 79268357 ID=MsG0480022968.01.T01;Parent=MsG0480022968.01;Name=MsG0480022968.01.T01;_AED=0.37;_eAED=0.37;_QI=0|1|1|1|0|0|3|159|112
Chr4 exon 79268357 79268562 79268357 ID=MsG0480022968.01.T01:exon:11695;Parent=MsG0480022968.01.T01
Chr4 exon 79269468 79269624 79269468 ID=MsG0480022968.01.T01:exon:11694;Parent=MsG0480022968.01.T01
Chr4 exon 79270022 79270156 79270022 ID=MsG0480022968.01.T01:exon:11693;Parent=MsG0480022968.01.T01
Chr4 CDS 79270022 79270156 79270022 ID=MsG0480022968.01.T01:cds;Parent=MsG0480022968.01.T01
Chr4 CDS 79269468 79269624 79269468 ID=MsG0480022968.01.T01:cds;Parent=MsG0480022968.01.T01
Chr4 CDS 79268516 79268562 79268516 ID=MsG0480022968.01.T01:cds;Parent=MsG0480022968.01.T01
Chr4 three_prime_UTR 79268357 79268515 79268357 ID=MsG0480022968.01.T01:three_prime_utr;Parent=MsG0480022968.01.T01
Gene Sequence

>MsG0480022968.01.T01

ACAGATATTCAGAAATCTCTAAATAAAACCTCGGAGAGAGTCACTGCCGGAAAAGAACATCACCACCGTCTTTCAATTTTCCGGCGTTCTCTTCGTTTTTCCACCGGCGATCCATTATCCTACAAATCAATCATGACTACATCAAAGCGCCTTGCAGTTAGGAAGGTTGAGAAGTTCGAGAAAAATATCACAAAAAGAGGAAGTGTGCCAGAAACAACTGTTAAGAAAGGAAATGACTATCCTGTTGGCCCCGTTTTGCTTGGTTTCTTCGTCTTTGTTGTCATTGGATCATCTCTCTTTCAGATAATCAGGACAGCAACGAGTGGAGGCATGGCATAA

Protein sequence

>MsG0480022968.01.T01

TDIQKSLNKTSERVTAGKEHHHRLSIFRRSLRFSTGDPLSYKSIMTTSKRLAVRKVEKFEKNITKRGSVPETTVKKGNDYPVGPVLLGFFVFVVIGSSLFQIIRTATSGGMA*