Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048874.01 | MsG0280010117.01 | 0.802383 | 5.757969e-49 | 2.816770e-46 |
| MsG0180000470.01 | MsG0280010117.01 | 0.819832 | 9.443468e-53 | 7.347331e-50 |
| MsG0180002233.01 | MsG0280010117.01 | 0.803913 | 2.777601e-49 | 1.413068e-46 |
| MsG0180002282.01 | MsG0280010117.01 | 0.814631 | 1.396339e-51 | 9.418477e-49 |
| MsG0180002667.01 | MsG0280010117.01 | 0.806980 | 6.320606e-50 | 3.478804e-47 |
| MsG0180004275.01 | MsG0280010117.01 | 0.811999 | 5.285984e-51 | 3.321178e-48 |
| MsG0280006325.01 | MsG0280010117.01 | 0.812589 | 3.928491e-51 | 2.507814e-48 |
| MsG0280006510.01 | MsG0280010117.01 | 0.811118 | 8.213125e-51 | 5.041256e-48 |
| MsG0280009248.01 | MsG0280010117.01 | 0.810874 | 9.278802e-51 | 5.658867e-48 |
| MsG0280010117.01 | MsG0380011617.01 | 0.806328 | 8.677951e-50 | 4.696953e-47 |
| MsG0280010117.01 | MsG0380012009.01 | 0.808945 | 2.413276e-50 | 1.398306e-47 |
| MsG0280010117.01 | MsG0380016274.01 | 0.813768 | 2.165097e-51 | 1.426573e-48 |
| MsG0280010117.01 | MsG0380017326.01 | 0.811679 | 6.204812e-51 | 3.865070e-48 |
| MsG0280010117.01 | MsG0480018121.01 | 0.807605 | 4.658092e-50 | 2.605655e-47 |
| MsG0280010117.01 | MsG0480018664.01 | 0.838720 | 2.432275e-57 | 3.286063e-54 |
| MsG0280010117.01 | MsG0480021587.01 | 0.800832 | 1.197556e-48 | 5.632246e-46 |
| MsG0280010117.01 | MsG0580026434.01 | 0.807734 | 4.373763e-50 | 2.454994e-47 |
| MsG0280010117.01 | MsG0580026613.01 | 0.804982 | 1.663439e-49 | 8.697981e-47 |
| MsG0280010117.01 | MsG0580027838.01 | 0.800533 | 1.378413e-48 | 6.433621e-46 |
| MsG0280010117.01 | MsG0580028169.01 | 0.823919 | 1.068723e-53 | 9.328210e-51 |
| MsG0280010117.01 | MsG0580028170.01 | 0.834917 | 2.271379e-56 | 2.734506e-53 |
| MsG0280010117.01 | MsG0680032157.01 | 0.800760 | 1.238855e-48 | 5.815608e-46 |
| MsG0280010117.01 | MsG0680032158.01 | 0.826138 | 3.197921e-54 | 2.973560e-51 |
| MsG0280010117.01 | MsG0680035444.01 | 0.801525 | 8.641683e-49 | 4.136032e-46 |
| MsG0280010117.01 | MsG0780036201.01 | 0.815113 | 1.091984e-51 | 7.461468e-49 |
| MsG0280010117.01 | MsG0780037606.01 | 0.831838 | 1.330438e-55 | 1.461153e-52 |
| MsG0280010117.01 | MsG0780038439.01 | 0.803099 | 4.096568e-49 | 2.040694e-46 |
| MsG0280010117.01 | MsG0780039880.01 | 0.807537 | 4.815740e-50 | 2.689161e-47 |
| MsG0280010117.01 | MsG0880042454.01 | 0.807197 | 5.686326e-50 | 3.147397e-47 |
| MsG0280010117.01 | MsG0880043812.01 | 0.823206 | 1.568936e-53 | 1.342093e-50 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 12 sgRNAs with CRISPR-Local
Find 17 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCTCAGGTCCCATCCTTTC+AGG | 0.164802 | 2:+64763842 | MsG0280010117.01.T01:CDS |
| TTTATTTCATTCACTTGTTT+TGG | 0.234633 | 2:+64763814 | MsG0280010117.01.T01:CDS |
| ATATAGATGATGGGTTTGAT+TGG | 0.393563 | 2:+64763938 | MsG0280010117.01.T01:CDS |
| GATGATGGGTTTGATTGGTT+TGG | 0.407984 | 2:+64763943 | MsG0280010117.01.T01:CDS |
| GGTTTGGTGATGAAGTTTGC+TGG | 0.429867 | 2:+64763959 | MsG0280010117.01.T01:CDS |
| TTGACAAATTCCTGAAAGGA+TGG | 0.497818 | 2:-64763852 | None:intergenic |
| TTTGGTTACATATAGATGAT+GGG | 0.522179 | 2:+64763929 | MsG0280010117.01.T01:CDS |
| AGAACGACGTTGATTGTGAA+AGG | 0.578265 | 2:+64764024 | MsG0280010117.01.T01:CDS |
| AGACGCATTGAGAAAATCAA+AGG | 0.589980 | 2:+64763745 | MsG0280010117.01.T01:CDS |
| ATAGTTGACAAATTCCTGAA+AGG | 0.595751 | 2:-64763856 | None:intergenic |
| GAATTCAAACACAAAATCAT+CGG | 0.605670 | 2:-64763884 | None:intergenic |
| TGACAAATTCCTGAAAGGAT+GGG | 0.619729 | 2:-64763851 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTATTTCATTCACTTGTTT+TGG | + | Chr2:64763814-64763833 | MsG0280010117.01.T01:CDS | 20.0% |
| ! | GAATTCAAACACAAAATCAT+CGG | - | Chr2:64763887-64763906 | None:intergenic | 25.0% |
| ! | TTTGGTTACATATAGATGAT+GGG | + | Chr2:64763929-64763948 | MsG0280010117.01.T01:CDS | 25.0% |
| !!! | CTTGCTATGATTTTCAATTT+TGG | + | Chr2:64763911-64763930 | MsG0280010117.01.T01:CDS | 25.0% |
| !!! | TTTTGGTTACATATAGATGA+TGG | + | Chr2:64763928-64763947 | MsG0280010117.01.T01:CDS | 25.0% |
| ATAGTTGACAAATTCCTGAA+AGG | - | Chr2:64763859-64763878 | None:intergenic | 30.0% | |
| TCCAAAAATCAAGAATGCAA+AGG | - | Chr2:64763998-64764017 | None:intergenic | 30.0% | |
| ! | TCCTTTGCATTCTTGATTTT+TGG | + | Chr2:64763994-64764013 | MsG0280010117.01.T01:CDS | 30.0% |
| !! | ATATAGATGATGGGTTTGAT+TGG | + | Chr2:64763938-64763957 | MsG0280010117.01.T01:CDS | 30.0% |
| AGACGCATTGAGAAAATCAA+AGG | + | Chr2:64763745-64763764 | MsG0280010117.01.T01:CDS | 35.0% | |
| TGACAAATTCCTGAAAGGAT+GGG | - | Chr2:64763854-64763873 | None:intergenic | 35.0% | |
| TTGACAAATTCCTGAAAGGA+TGG | - | Chr2:64763855-64763874 | None:intergenic | 35.0% | |
| !!! | CTTGTTTTGGTTCAATTCTC+AGG | + | Chr2:64763827-64763846 | MsG0280010117.01.T01:CDS | 35.0% |
| ! | AGAACGACGTTGATTGTGAA+AGG | + | Chr2:64764024-64764043 | MsG0280010117.01.T01:CDS | 40.0% |
| !! | GATGATGGGTTTGATTGGTT+TGG | + | Chr2:64763943-64763962 | MsG0280010117.01.T01:CDS | 40.0% |
| !! | GGTTTGGTGATGAAGTTTGC+TGG | + | Chr2:64763959-64763978 | MsG0280010117.01.T01:CDS | 45.0% |
| TTCTCAGGTCCCATCCTTTC+AGG | + | Chr2:64763842-64763861 | MsG0280010117.01.T01:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 64763688 | 64764056 | 64763688 | ID=MsG0280010117.01;Name=MsG0280010117.01 |
| Chr2 | mRNA | 64763688 | 64764056 | 64763688 | ID=MsG0280010117.01.T01;Parent=MsG0280010117.01;Name=MsG0280010117.01.T01;_AED=0.42;_eAED=0.42;_QI=0|-1|0|1|-1|0|1|0|122 |
| Chr2 | exon | 64763688 | 64764056 | 64763688 | ID=MsG0280010117.01.T01:exon:8331;Parent=MsG0280010117.01.T01 |
| Chr2 | CDS | 64763688 | 64764056 | 64763688 | ID=MsG0280010117.01.T01:cds;Parent=MsG0280010117.01.T01 |
| Gene Sequence |
| Protein sequence |
