AlfalfaGEDB | MsG0580030173.01

M. sativa cultivar ZhongmuNo.1 / MsG0580030173.01

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580030173.01.T01	RHN58171.1	83.696	184	22	5	1	180	11	190	1.72E-78	243

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580030173.01.T01	A0A396I020	83.696	184	22	5	1	180	11	190	8.22e-79	243

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180003281.01	MsG0580030173.01	0.811467	6.899651e-51	4.274260e-48
MsG0180004320.01	MsG0580030173.01	0.800703	1.272365e-48	5.964227e-46
MsG0280011258.01	MsG0580030173.01	0.809420	1.908860e-50	1.120255e-47
MsG0280011282.01	MsG0580030173.01	0.815173	1.058577e-51	7.245078e-49
MsG0380011925.01	MsG0580030173.01	0.806919	6.511121e-50	3.577976e-47
MsG0380012471.01	MsG0580030173.01	0.809553	1.787174e-50	1.052542e-47
MsG0480021350.01	MsG0580030173.01	0.805549	1.265480e-49	6.713944e-47
MsG0480022325.01	MsG0580030173.01	0.804478	2.119397e-49	1.093854e-46
MsG0380015677.01	MsG0580030173.01	0.833583	4.906130e-56	5.674536e-53
MsG0380016994.01	MsG0580030173.01	0.812779	3.570103e-51	2.290316e-48
MsG0580029640.01	MsG0580030173.01	0.817333	3.481879e-52	2.528369e-49
MsG0580030173.01	MsG0780036752.01	0.807696	4.455932e-50	2.498479e-47
MsG0580030173.01	MsG0780038424.01	0.814457	1.525925e-51	1.024413e-48
MsG0580030173.01	MsG0880042961.01	0.824827	6.535055e-54	5.854214e-51
MsG0580030173.01	MsG0880046012.01	0.802267	6.084141e-49	2.967374e-46
MsG0280007609.01	MsG0580030173.01	0.811621	6.388099e-51	3.973293e-48
MsG0280008280.01	MsG0580030173.01	0.823560	1.297219e-53	1.120769e-50
MsG0280009514.01	MsG0580030173.01	0.808300	3.313644e-50	1.887835e-47
MsG0280010491.01	MsG0580030173.01	0.833195	6.132745e-56	7.011504e-53

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580030173.01.T01	MTR_5g098480	82.873	181	14	6	1	180	11	175	3.68e-79	235

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 14 sgRNAs with CRISPR-Local

Find 20 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GAAGAAGAAGTTAATATTGA+TGG	0.304002	5:-107759579	MsG0580030173.01.T01:CDS
ATCTTTGCTCTACACATCTA+TGG	0.308989	5:-107759735	MsG0580030173.01.T01:CDS
CAATCCAACCAGAAGGTAAC+TGG	0.337395	5:+107759764	None:intergenic
TTTCAGTCAACAAAGTCTAA+TGG	0.361434	5:-107759349	MsG0580030173.01.T01:CDS
CAGTTACCTTCTGGTTGGAT+TGG	0.384196	5:-107759763	MsG0580030173.01.T01:CDS
CAGAAGTTGACAAATGATCT+TGG	0.395226	5:+107759541	None:intergenic
TTGATCCATCTGAGTAATCA+TGG	0.491540	5:+107759496	None:intergenic
CATAGCCATGATTACTCAGA+TGG	0.516223	5:-107759501	MsG0580030173.01.T01:CDS
AGATCATTTGTCAACTTCTG+AGG	0.525772	5:-107759538	MsG0580030173.01.T01:CDS
AGAACTCAATGAAGTGAATG+AGG	0.548433	5:-107759316	MsG0580030173.01.T01:CDS
GATCATTTGTCAACTTCTGA+GGG	0.561459	5:-107759537	MsG0580030173.01.T01:CDS
ATTGAAATTGCACTTCCAAG+TGG	0.592923	5:-107759453	MsG0580030173.01.T01:CDS
ATATCCACATAGTTTCCACT+TGG	0.606905	5:+107759438	None:intergenic
ACTTCCAAGTGGAAACTATG+TGG	0.636002	5:-107759442	MsG0580030173.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	CAGAAGAAAAAGAAGTTATT+TGG	-	Chr5:107759776-107759795	MsG0580030173.01.T01:CDS	25.0%
!	GAAGAAGAAGTTAATATTGA+TGG	-	Chr5:107759578-107759597	MsG0580030173.01.T01:CDS	25.0%
	TTTCAGTCAACAAAGTCTAA+TGG	-	Chr5:107759808-107759827	MsG0580030173.01.T01:CDS	30.0%
!	GTTCTATTTTCTCATCTTCA+TGG	-	Chr5:107759339-107759358	MsG0580030173.01.T01:CDS	30.0%
!	TATTTTCTCATCTTCATGGA+TGG	-	Chr5:107759343-107759362	MsG0580030173.01.T01:CDS	30.0%
	AGAACTCAATGAAGTGAATG+AGG	-	Chr5:107759841-107759860	MsG0580030173.01.T01:CDS	35.0%
	AGATCATTTGTCAACTTCTG+AGG	-	Chr5:107759619-107759638	MsG0580030173.01.T01:CDS	35.0%
	ATATCCACATAGTTTCCACT+TGG	+	Chr5:107759722-107759741	None:intergenic	35.0%
	ATCTTTGCTCTACACATCTA+TGG	-	Chr5:107759422-107759441	MsG0580030173.01.T01:CDS	35.0%
	ATTGAAATTGCACTTCCAAG+TGG	-	Chr5:107759704-107759723	MsG0580030173.01.T01:CDS	35.0%
	CAGAAGTTGACAAATGATCT+TGG	+	Chr5:107759619-107759638	None:intergenic	35.0%
	GATCATTTGTCAACTTCTGA+GGG	-	Chr5:107759620-107759639	MsG0580030173.01.T01:CDS	35.0%
	TAAAAACCAATCCAACCAGA+AGG	+	Chr5:107759403-107759422	None:intergenic	35.0%
	TTGATCCATCTGAGTAATCA+TGG	+	Chr5:107759664-107759683	None:intergenic	35.0%
!!!	TCTTTTTTCCAGTTACCTTC+TGG	-	Chr5:107759385-107759404	MsG0580030173.01.T01:CDS	35.0%
	ACTTCCAAGTGGAAACTATG+TGG	-	Chr5:107759715-107759734	MsG0580030173.01.T01:CDS	40.0%
	CATAGCCATGATTACTCAGA+TGG	-	Chr5:107759656-107759675	MsG0580030173.01.T01:CDS	40.0%
!	TTTTCCAGTTACCTTCTGGT+TGG	-	Chr5:107759389-107759408	MsG0580030173.01.T01:CDS	40.0%
	CAATCCAACCAGAAGGTAAC+TGG	+	Chr5:107759396-107759415	None:intergenic	45.0%
!	CAGTTACCTTCTGGTTGGAT+TGG	-	Chr5:107759394-107759413	MsG0580030173.01.T01:CDS	45.0%

Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	107759302	107759877	107759302	ID=MsG0580030173.01;Name=MsG0580030173.01
Chr5	mRNA	107759302	107759877	107759302	ID=MsG0580030173.01.T01;Parent=MsG0580030173.01;Name=MsG0580030173.01.T01;_AED=0.25;_eAED=0.25;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|191
Chr5	exon	107759302	107759877	107759302	ID=MsG0580030173.01.T01:exon:14942;Parent=MsG0580030173.01.T01
Chr5	CDS	107759302	107759877	107759302	ID=MsG0580030173.01.T01:cds;Parent=MsG0580030173.01.T01

Gene Sequence

>MsG0580030173.01.T01

ATGTTTTCTTATGATTTGTGCATATTCAAGAGTTTTTGTTCTATTTTCTCATCTTCATGGATGGCTCTTTTTGCTTTATTGCTTCTTTTTTCCAGTTACCTTCTGGTTGGATTGGTTTTTATCTTTGCTCTACACATCTATGGTAGTAATTTTTTCTACTTTTCTCTATATTATTATTGCATCAAAGCAAAAAAAGTAAATGTTGTTGATAAATTAGTAGAAGAAAAGTTTCTGAATTCAGATGATGAGAAAAGTAATGAAGCACATGAAGCACAAGAAGAAGAAGTTAATATTGATGGATCAGAAAGTGAAAGCCAAGATCATTTGTCAACTTCTGAGGGATCTGAAGTAGATCATAGCCATGATTACTCAGATGGATCAATTTCTGATGAAGAAAGTCTCATTGAAATTGCACTTCCAAGTGGAAACTATGTGGATATGCAGAAAGATGAATCAGATTACTATAGTTTGCAGCAGAAGAAAAAGAAGTTATTTGGTGATGCACTTTTCAGTCAACAAAGTCTAATGGAGTTGTTATCAGAACTCAATGAAGTGAATGAGGAAAAAACTTTATAG

Protein sequence

>MsG0580030173.01.T01

MFSYDLCIFKSFCSIFSSSWMALFALLLLFSSYLLVGLVFIFALHIYGSNFFYFSLYYYCIKAKKVNVVDKLVEEKFLNSDDEKSNEAHEAQEEEVNIDGSESESQDHLSTSEGSEVDHSHDYSDGSISDEESLIEIALPSGNYVDMQKDESDYYSLQQKKKKLFGDALFSQQSLMELLSELNEVNEEKTL*