AlfalfaGEDB | MsG0380011925.01

M. sativa cultivar ZhongmuNo.1 / MsG0380011925.01

KEGG: -
GO: -
PFAM: PF06697

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380011925.01.T01	XP_003598365.1	98.154	325	4	1	1	323	1	325	0	661

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380011925.01.T01	G7IVH8	98.154	325	4	1	1	323	1	325	0.0	661

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080047814.01	MsG0380011925.01	0.808241	3.412411e-50	1.941126e-47
MsG0080048461.01	MsG0380011925.01	0.801973	6.992799e-49	3.384879e-46
MsG0080048856.01	MsG0380011925.01	0.817411	3.343664e-52	2.433332e-49
MsG0180001006.01	MsG0380011925.01	0.802668	5.029111e-49	2.477931e-46
MsG0180003361.01	MsG0380011925.01	0.811569	6.555320e-51	4.071852e-48
MsG0180004099.01	MsG0380011925.01	0.814314	1.640805e-51	1.097325e-48
MsG0180004754.01	MsG0380011925.01	0.807566	4.747020e-50	2.652767e-47
MsG0180004970.01	MsG0380011925.01	0.825439	4.683887e-54	4.269185e-51
MsG0280011013.01	MsG0380011925.01	0.802127	6.499985e-49	3.158877e-46
MsG0280011014.01	MsG0380011925.01	0.821696	3.518930e-53	2.883808e-50
MsG0280011258.01	MsG0380011925.01	0.811791	5.865777e-51	3.664955e-48
MsG0280011282.01	MsG0380011925.01	0.842579	2.372956e-58	3.613858e-55
MsG0380011803.01	MsG0380011925.01	0.881551	2.184652e-70	1.285731e-66
MsG0380011925.01	MsG0380012471.01	0.854140	1.500770e-61	3.319726e-58
MsG0380011925.01	MsG0380013475.01	0.823020	1.733685e-53	1.475356e-50
MsG0380011925.01	MsG0380013549.01	0.813100	3.036263e-51	1.964496e-48
MsG0380011925.01	MsG0380013834.01	0.805897	1.069289e-49	5.724305e-47
MsG0380011925.01	MsG0380014703.01	0.822663	2.100417e-53	1.769369e-50
MsG0380011925.01	MsG0380015677.01	0.829166	5.991278e-55	6.083470e-52
MsG0380011925.01	MsG0380016553.01	0.806493	8.008468e-50	4.352876e-47
MsG0380011925.01	MsG0380016765.01	0.800038	1.738153e-48	8.011488e-46
MsG0380011925.01	MsG0380016994.01	0.834354	3.146960e-56	3.725220e-53
MsG0380011925.01	MsG0480018313.01	0.821988	3.013174e-53	2.489728e-50
MsG0380011925.01	MsG0480018919.01	0.805903	1.066370e-49	5.709475e-47
MsG0380011925.01	MsG0480022325.01	0.815413	9.365794e-52	6.452635e-49
MsG0380011925.01	MsG0480022620.01	0.803798	2.935699e-49	1.488962e-46
MsG0380011925.01	MsG0480023832.01	0.821999	2.995053e-53	2.475609e-50
MsG0380011925.01	MsG0580024489.01	0.808141	3.582851e-50	2.032701e-47
MsG0380011925.01	MsG0580026593.01	0.806854	6.721165e-50	3.687283e-47
MsG0380011925.01	MsG0580026610.01	0.801872	7.333407e-49	3.541063e-46
MsG0380011925.01	MsG0580027431.01	0.801284	9.682817e-49	4.606273e-46
MsG0380011925.01	MsG0580028176.01	0.814550	1.455397e-51	9.795304e-49
MsG0380011925.01	MsG0580029640.01	0.826876	2.132025e-54	2.024733e-51
MsG0380011925.01	MsG0580030173.01	0.806919	6.511121e-50	3.577976e-47
MsG0380011925.01	MsG0680035692.01	0.805950	1.042040e-49	5.586042e-47
MsG0380011925.01	MsG0780036477.01	0.830352	3.082090e-55	3.239255e-52
MsG0380011925.01	MsG0780036663.01	0.805431	1.339122e-49	7.083426e-47
MsG0380011925.01	MsG0780036719.01	0.802729	4.886014e-49	2.411348e-46
MsG0380011925.01	MsG0780036752.01	0.838724	2.426452e-57	3.278588e-54
MsG0380011925.01	MsG0780038424.01	0.829744	4.337116e-55	4.479389e-52
MsG0380011925.01	MsG0780039059.01	0.800031	1.744377e-48	8.038625e-46
MsG0380011925.01	MsG0780039137.01	0.836112	1.132569e-56	1.413364e-53
MsG0380011925.01	MsG0780039243.01	0.804378	2.223670e-49	1.144763e-46
MsG0380011925.01	MsG0780040841.01	0.835282	1.837881e-56	2.237102e-53
MsG0380011925.01	MsG0780041535.01	0.829171	5.975337e-55	6.068272e-52
MsG0380011925.01	MsG0880041860.01	0.810754	9.849658e-51	5.987731e-48
MsG0380011925.01	MsG0880042961.01	0.805659	1.199961e-49	6.384578e-47
MsG0380011925.01	MsG0880043491.01	0.814781	1.293216e-51	8.757921e-49
MsG0380011925.01	MsG0880045145.01	0.803254	3.805440e-49	1.903312e-46
MsG0380011925.01	MsG0880046012.01	0.864324	1.319516e-64	4.126063e-61
MsG0380011925.01	MsG0880046479.01	0.802355	5.835241e-49	2.852510e-46
MsG0380011925.01	MsG0880047473.01	0.804555	2.041789e-49	1.055927e-46
MsG0280007609.01	MsG0380011925.01	0.805862	1.087309e-49	5.815777e-47
MsG0280008280.01	MsG0380011925.01	0.807788	4.260069e-50	2.394478e-47
MsG0280008615.01	MsG0380011925.01	0.811871	5.636040e-51	3.528858e-48
MsG0280008909.01	MsG0380011925.01	0.801354	9.369276e-49	4.464984e-46
MsG0280009514.01	MsG0380011925.01	0.811569	6.557314e-51	4.073038e-48
MsG0280009794.01	MsG0380011925.01	0.807775	4.287789e-50	2.409207e-47
MsG0280010176.01	MsG0380011925.01	0.809425	1.904542e-50	1.117847e-47
MsG0280010491.01	MsG0380011925.01	0.853939	1.715223e-61	3.768338e-58

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380011925.01.T01	MTR_3g010800	98.154	325	4	1	1	323	1	325	0.0	661
MsG0380011925.01.T01	MTR_3g062840	34.743	331	169	14	7	318	8	310	1.49e-38	139
MsG0380011925.01.T01	MTR_5g075750	33.125	320	190	12	1	318	4	301	1.79e-37	135
MsG0380011925.01.T01	MTR_8g461170	29.450	309	187	10	17	318	10	294	2.70e-29	114
MsG0380011925.01.T01	MTR_7g074120	28.660	321	199	9	7	317	13	313	4.55e-27	108
MsG0380011925.01.T01	MTR_8g028165	27.465	284	177	10	37	314	34	294	1.71e-21	92.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380011925.01.T01	AT4G22900	47.059	340	149	9	1	317	1	332	1.18e-97	292
MsG0380011925.01.T01	AT4G11950	42.814	334	156	9	8	317	4	326	2.58e-85	260
MsG0380011925.01.T01	AT1G62981	40.588	340	170	13	3	318	10	341	2.36e-64	207
MsG0380011925.01.T01	AT1G62981	40.588	340	170	13	3	318	10	341	2.36e-64	207
MsG0380011925.01.T01	AT3G08600	32.550	298	161	13	33	313	37	311	1.43e-22	96.3
MsG0380011925.01.T01	AT4G23720	27.397	292	185	13	26	313	38	306	3.16e-18	84.0
MsG0380011925.01.T01	AT4G01140	27.645	293	186	12	27	316	28	297	1.79e-16	79.0
MsG0380011925.01.T01	AT1G62981	35.676	185	102	8	146	318	29	208	3.08e-16	76.6

Find 41 sgRNAs with CRISPR-Local

Find 50 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCTAAAACACATGGACATTT+TGG	0.175170	3:+8047568	MsG0380011925.01.T01:CDS
TTCCATTGACTTAGTCTTAT+AGG	0.189682	3:-8047637	None:intergenic
AGTACTATTGGTGCTGCTTT+AGG	0.264423	3:+8047673	MsG0380011925.01.T01:CDS
AAGCTACCTTCAAATTTATC+AGG	0.315618	3:+8047100	MsG0380011925.01.T01:CDS
CCTTCAAAACTTGCACATAA+TGG	0.317349	3:-8047490	None:intergenic
CAACTTGTTTCTCCAATTGT+TGG	0.336972	3:+8047319	MsG0380011925.01.T01:CDS
TAAGCTAAAATTCCAACAAT+TGG	0.343703	3:-8047331	None:intergenic
TTGTTGTCATTGGAATTTCA+TGG	0.351140	3:+8046968	MsG0380011925.01.T01:CDS
TTCAACAACCAAGAAGAAAA+AGG	0.352105	3:+8047454	MsG0380011925.01.T01:CDS
GTTGGTGTTGTTCTAGTTCC+AGG	0.353242	3:-8047838	None:intergenic
TTATGAAGAAGAAGCACTTC+AGG	0.366104	3:+8047780	MsG0380011925.01.T01:CDS
GCATAGAGTATAATAATAAT+TGG	0.373049	3:+8047254	MsG0380011925.01.T01:CDS
AAGGTATGGTGCAAAATTAA+AGG	0.383254	3:+8047165	MsG0380011925.01.T01:CDS
AAGGAGTTTCATCTTGGTAT+TGG	0.399934	3:+8047184	MsG0380011925.01.T01:CDS
ATTGCAGTTGGGAGTACTAT+TGG	0.400361	3:+8047661	MsG0380011925.01.T01:CDS
TTGATGCCTGATAAATTTGA+AGG	0.417038	3:-8047106	None:intergenic
ACACAAACATTTGTTGCTAA+AGG	0.423104	3:-8047544	None:intergenic
GCACTTCAGGTTTCAATGGT+TGG	0.434477	3:+8047793	MsG0380011925.01.T01:CDS
AGAAGCACTTCAGGTTTCAA+TGG	0.442190	3:+8047789	MsG0380011925.01.T01:CDS
GCTTCAATTCCAAGTTGAAA+AGG	0.444070	3:-8047388	None:intergenic
AAATTAAAGGAGTTTCATCT+TGG	0.452708	3:+8047178	MsG0380011925.01.T01:CDS
AACATTACTCTTTCAATACA+TGG	0.463603	3:-8047220	None:intergenic
TTTCAACTTGGAATTGAAGC+TGG	0.480245	3:+8047391	MsG0380011925.01.T01:CDS
TTAGACAAAGATTTCAAGAC+AGG	0.484697	3:+8047061	MsG0380011925.01.T01:CDS
GTTCTAGTTCCAGGTGCAGT+AGG	0.488529	3:-8047829	None:intergenic
TGAATGTGTAATCTCTTATG+AGG	0.490113	3:-8047033	None:intergenic
GTCAATGGAAAATTGCAGTT+GGG	0.491640	3:+8047650	MsG0380011925.01.T01:CDS
AGCCTATAAGACTAAGTCAA+TGG	0.492496	3:+8047635	MsG0380011925.01.T01:CDS
GGTTAAGAAAAGGTCAAGAA+TGG	0.493692	3:+8047738	MsG0380011925.01.T01:CDS
GAAGGTAGCTTCACTGATTG+TGG	0.501570	3:-8047088	None:intergenic
TGTGGAAGTTTAACAAGGTA+TGG	0.509899	3:+8047151	MsG0380011925.01.T01:CDS
AGTCAATGGAAAATTGCAGT+TGG	0.516447	3:+8047649	MsG0380011925.01.T01:CDS
AAGGTCAAGAATGGTTCAGA+TGG	0.524303	3:+8047747	MsG0380011925.01.T01:CDS
TGGTTGTTAGGGTTAAGAAA+AGG	0.555158	3:+8047728	MsG0380011925.01.T01:CDS
GTGAGAGCTCCTACTGCACC+TGG	0.585262	3:+8047820	MsG0380011925.01.T01:CDS
GTTTGTGTTGCTAAAACACA+TGG	0.605683	3:+8047559	MsG0380011925.01.T01:CDS
TTAGATGTGGAAGTTTAACA+AGG	0.623871	3:+8047146	MsG0380011925.01.T01:CDS
GATACTGTGAGATTTAGATG+TGG	0.646729	3:+8047133	MsG0380011925.01.T01:CDS
TAGTCATGTTGCTCAAGTGT+TGG	0.655160	3:-8047856	None:intergenic
ATGTGTAATCTCTTATGAGG+AGG	0.665772	3:-8047030	None:intergenic
TGCAGTAAACCTTATCAACG+AGG	0.756012	3:-8047892	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	GCATAGAGTATAATAATAAT+TGG	+	Chr3:8047254-8047273	MsG0380011925.01.T01:CDS	20.0%
!	AAATTAAAGGAGTTTCATCT+TGG	+	Chr3:8047178-8047197	MsG0380011925.01.T01:CDS	25.0%
!	AACATTACTCTTTCAATACA+TGG	-	Chr3:8047223-8047242	None:intergenic	25.0%
!	TAAGCTAAAATTCCAACAAT+TGG	-	Chr3:8047334-8047353	None:intergenic	25.0%
	AAGCTACCTTCAAATTTATC+AGG	+	Chr3:8047100-8047119	MsG0380011925.01.T01:CDS	30.0%
	AAGGTATGGTGCAAAATTAA+AGG	+	Chr3:8047165-8047184	MsG0380011925.01.T01:CDS	30.0%
	TGAATGTGTAATCTCTTATG+AGG	-	Chr3:8047036-8047055	None:intergenic	30.0%
	TTAGACAAAGATTTCAAGAC+AGG	+	Chr3:8047061-8047080	MsG0380011925.01.T01:CDS	30.0%
	TTAGATGTGGAAGTTTAACA+AGG	+	Chr3:8047146-8047165	MsG0380011925.01.T01:CDS	30.0%
	TTCAACAACCAAGAAGAAAA+AGG	+	Chr3:8047454-8047473	MsG0380011925.01.T01:CDS	30.0%
	TTGATGCCTGATAAATTTGA+AGG	-	Chr3:8047109-8047128	None:intergenic	30.0%
	TTGTTGTCATTGGAATTTCA+TGG	+	Chr3:8046968-8046987	MsG0380011925.01.T01:CDS	30.0%
!	ACACAAACATTTGTTGCTAA+AGG	-	Chr3:8047547-8047566	None:intergenic	30.0%
!	AGTAGTAATCCTTTTCAACT+TGG	+	Chr3:8047379-8047398	MsG0380011925.01.T01:CDS	30.0%
!	CTTTGATACCTTTTTCTTCT+TGG	-	Chr3:8047465-8047484	None:intergenic	30.0%
!	GACATTTTCATTGTTGTCAT+TGG	+	Chr3:8046958-8046977	MsG0380011925.01.T01:CDS	30.0%
!	TTTTGTAGCAATGGTTGTTA+GGG	+	Chr3:8047717-8047736	MsG0380011925.01.T01:CDS	30.0%
!	TTTTTGTAGCAATGGTTGTT+AGG	+	Chr3:8047716-8047735	MsG0380011925.01.T01:CDS	30.0%
!!	TTCCATTGACTTAGTCTTAT+AGG	-	Chr3:8047640-8047659	None:intergenic	30.0%
!!!	AGGTTTACTTTTTGTAGCAA+TGG	+	Chr3:8047708-8047727	MsG0380011925.01.T01:CDS	30.0%
	AAGGAGTTTCATCTTGGTAT+TGG	+	Chr3:8047184-8047203	MsG0380011925.01.T01:CDS	35.0%
	AGCCTATAAGACTAAGTCAA+TGG	+	Chr3:8047635-8047654	MsG0380011925.01.T01:CDS	35.0%
	AGTCAATGGAAAATTGCAGT+TGG	+	Chr3:8047649-8047668	MsG0380011925.01.T01:CDS	35.0%
	ATGTGTAATCTCTTATGAGG+AGG	-	Chr3:8047033-8047052	None:intergenic	35.0%
	CCTTCAAAACTTGCACATAA+TGG	-	Chr3:8047493-8047512	None:intergenic	35.0%
	GATACTGTGAGATTTAGATG+TGG	+	Chr3:8047133-8047152	MsG0380011925.01.T01:CDS	35.0%
	GGTTAAGAAAAGGTCAAGAA+TGG	+	Chr3:8047738-8047757	MsG0380011925.01.T01:CDS	35.0%
	GTCAATGGAAAATTGCAGTT+GGG	+	Chr3:8047650-8047669	MsG0380011925.01.T01:CDS	35.0%
	TGGTTGTTAGGGTTAAGAAA+AGG	+	Chr3:8047728-8047747	MsG0380011925.01.T01:CDS	35.0%
	TGTGGAAGTTTAACAAGGTA+TGG	+	Chr3:8047151-8047170	MsG0380011925.01.T01:CDS	35.0%
	TTTCAACTTGGAATTGAAGC+TGG	+	Chr3:8047391-8047410	MsG0380011925.01.T01:CDS	35.0%
!	CAACTTGTTTCTCCAATTGT+TGG	+	Chr3:8047319-8047338	MsG0380011925.01.T01:CDS	35.0%
!	GCTAAAACACATGGACATTT+TGG	+	Chr3:8047568-8047587	MsG0380011925.01.T01:CDS	35.0%
!	GCTTCAATTCCAAGTTGAAA+AGG	-	Chr3:8047391-8047410	None:intergenic	35.0%
!	GTTTGTGTTGCTAAAACACA+TGG	+	Chr3:8047559-8047578	MsG0380011925.01.T01:CDS	35.0%
!!	CCATTATGTGCAAGTTTTGA+AGG	+	Chr3:8047490-8047509	MsG0380011925.01.T01:CDS	35.0%
!!	GCTTTAGGTGCTTTTCTTTT+AGG	+	Chr3:8047688-8047707	MsG0380011925.01.T01:CDS	35.0%
!!	TTATGAAGAAGAAGCACTTC+AGG	+	Chr3:8047780-8047799	MsG0380011925.01.T01:CDS	35.0%
!!!	AACACATGGACATTTTGGTT+TGG	+	Chr3:8047573-8047592	MsG0380011925.01.T01:CDS	35.0%
!!!	TGTGCAAGTTTTGAAGGAAA+TGG	+	Chr3:8047496-8047515	MsG0380011925.01.T01:CDS	35.0%
	AAGGTCAAGAATGGTTCAGA+TGG	+	Chr3:8047747-8047766	MsG0380011925.01.T01:CDS	40.0%
	TAGTCATGTTGCTCAAGTGT+TGG	-	Chr3:8047859-8047878	None:intergenic	40.0%
!!	AGAAGCACTTCAGGTTTCAA+TGG	+	Chr3:8047789-8047808	MsG0380011925.01.T01:CDS	40.0%
!!	AGTACTATTGGTGCTGCTTT+AGG	+	Chr3:8047673-8047692	MsG0380011925.01.T01:CDS	40.0%
!!	ATTGCAGTTGGGAGTACTAT+TGG	+	Chr3:8047661-8047680	MsG0380011925.01.T01:CDS	40.0%
!	GAAGGTAGCTTCACTGATTG+TGG	-	Chr3:8047091-8047110	None:intergenic	45.0%
!	GCACTTCAGGTTTCAATGGT+TGG	+	Chr3:8047793-8047812	MsG0380011925.01.T01:CDS	45.0%
!!	GTTGGTGTTGTTCTAGTTCC+AGG	-	Chr3:8047841-8047860	None:intergenic	45.0%
!!	GTTCTAGTTCCAGGTGCAGT+AGG	-	Chr3:8047832-8047851	None:intergenic	50.0%
	GTGAGAGCTCCTACTGCACC+TGG	+	Chr3:8047820-8047839	MsG0380011925.01.T01:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
Chr3	gene	8046929	8047900	8046929	ID=MsG0380011925.01;Name=MsG0380011925.01
Chr3	mRNA	8046929	8047900	8046929	ID=MsG0380011925.01.T01;Parent=MsG0380011925.01;Name=MsG0380011925.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|323
Chr3	exon	8046929	8047900	8046929	ID=MsG0380011925.01.T01:exon:3287;Parent=MsG0380011925.01.T01
Chr3	CDS	8046929	8047900	8046929	ID=MsG0380011925.01.T01:cds;Parent=MsG0380011925.01.T01

Gene Sequence

>MsG0380011925.01.T01

ATGGACTCATTTTACACTCTTATTTTGATGACATTTTCATTGTTGTCATTGGAATTTCATGGATCTTATGCTCAGAATATCAAATCAGTTCATGTTCTTGACCTCCTCATAAGAGATTACACATTCAAGTCATTAGACAAAGATTTCAAGACAGGAATACCACAATCAGTGAAGCTACCTTCAAATTTATCAGGCATCAAAGTAGATACTGTGAGATTTAGATGTGGAAGTTTAACAAGGTATGGTGCAAAATTAAAGGAGTTTCATCTTGGTATTGGTGTTTCTATTCATCCATGTATTGAAAGAGTAATGTTGATAAGACAAAGCATAGAGTATAATAATAATTGGTCTTCAATATATTATGCAAACTATATTGATCTATCTAAGTATCAACTTGTTTCTCCAATTGTTGGAATTTTAGCTTATAATGCTGATGAAGATTCAAACTCTAGTAGTAATCCTTTTCAACTTGGAATTGAAGCTGGTGAAAAACTCATCACAATTGATTTCAACAATGCTACAAATTTCAACAACCAAGAAGAAAAAGGTATCAAAGTGAAACCATTATGTGCAAGTTTTGAAGGAAATGGAAAAATGACACTTGCAATAACAAAACCTTTAGCAACAAATGTTTGTGTTGCTAAAACACATGGACATTTTGGTTTGGTTGTTGAGTCTTTACAACATCAAGATGATGAGTATAAAAAGCCTATAAGACTAAGTCAATGGAAAATTGCAGTTGGGAGTACTATTGGTGCTGCTTTAGGTGCTTTTCTTTTAGGTTTACTTTTTGTAGCAATGGTTGTTAGGGTTAAGAAAAGGTCAAGAATGGTTCAGATGGAAAGAAGAGCTTATGAAGAAGAAGCACTTCAGGTTTCAATGGTTGGACATGTGAGAGCTCCTACTGCACCTGGAACTAGAACAACACCAACACTTGAGCAACATGACTATATAACTCGACATCCTCGTTGA

Protein sequence

>MsG0380011925.01.T01

MDSFYTLILMTFSLLSLEFHGSYAQNIKSVHVLDLLIRDYTFKSLDKDFKTGIPQSVKLPSNLSGIKVDTVRFRCGSLTRYGAKLKEFHLGIGVSIHPCIERVMLIRQSIEYNNNWSSIYYANYIDLSKYQLVSPIVGILAYNADEDSNSSSNPFQLGIEAGEKLITIDFNNATNFNNQEEKGIKVKPLCASFEGNGKMTLAITKPLATNVCVAKTHGHFGLVVESLQHQDDEYKKPIRLSQWKIAVGSTIGAALGAFLLGLLFVAMVVRVKKRSRMVQMERRAYEEEALQVSMVGHVRAPTAPGTRTTPTLEQHDYITRHPR*