AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480021350.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021350.01.T01 MTR_4g123470 36.364 209 130 1 21 229 19 224 1.68e-41 141
MsG0480021350.01.T01 MTR_1g090753 44.138 145 77 2 3 147 19 159 1.06e-32 117
MsG0480021350.01.T01 MTR_1g054495 26.471 204 145 3 13 215 7 206 3.07e-14 70.1
MsG0480021350.01.T01 MTR_8g468550 42.857 77 44 0 34 110 65 141 1.73e-13 66.6
MsG0480021350.01.T01 MTR_0014s0220 23.280 189 126 3 29 217 642 811 1.66e-12 67.0
MsG0480021350.01.T01 MTR_7g025150 24.074 216 153 5 4 217 3 209 4.46e-12 63.9
MsG0480021350.01.T01 MTR_4g085790 25.463 216 150 3 4 217 3 209 4.59e-12 63.9
MsG0480021350.01.T01 MTR_7g039210 26.404 178 102 3 32 180 28 205 1.74e-11 62.4
MsG0480021350.01.T01 MTR_1g054485 22.346 179 139 0 9 187 4 182 1.74e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 34 sgRNAs with CRISPR-Local

Find 50 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGCCTAATATTGATGAATT+TGG 0.071532 4:+56940651 MsG0480021350.01.T01:CDS
TATAAGGCATTGTTAAAATT+TGG 0.194239 4:-56940440 None:intergenic
AAGAATTCCGCAATGAAAAT+TGG 0.202897 4:-56940351 None:intergenic
TGATGAATTTGGGACAAAAT+TGG 0.234698 4:+56940662 MsG0480021350.01.T01:CDS
TACTATGAAATCAATGATTA+TGG 0.240311 4:+56940492 MsG0480021350.01.T01:CDS
CATCTTCCTTTCATTCATAA+TGG 0.274505 4:+56940187 MsG0480021350.01.T01:CDS
GTCCCAAATTCATCAATATT+AGG 0.280924 4:-56940654 None:intergenic
GTTTAAGTGTGACTCAATTA+TGG 0.303074 4:+56940966 MsG0480021350.01.T01:CDS
TGCCTAATATTGATGAATTT+GGG 0.308974 4:+56940652 MsG0480021350.01.T01:CDS
CTAAATATGTAAAAGATATT+AGG 0.309400 4:-56940515 None:intergenic
ACCGTGGAAATTCGTTTGTT+TGG 0.318850 4:-56940578 None:intergenic
TCCCAACAAAAGGTAATATA+AGG 0.319342 4:-56940456 None:intergenic
TTGCATACAGTGATGAAATA+AGG 0.320941 4:-56940221 None:intergenic
GGAAATTCGTTTGTTTGGAT+TGG 0.329638 4:-56940573 None:intergenic
GAAATTCGTTTGTTTGGATT+GGG 0.339538 4:-56940572 None:intergenic
TGCGGAATTCTTCAATGAAC+TGG 0.345003 4:+56940362 MsG0480021350.01.T01:CDS
TTCCACATACACGTGTTTCA+TGG 0.348799 4:+56940549 MsG0480021350.01.T01:CDS
TTAAACTCTAAAATATCATT+TGG 0.369978 4:-56940949 None:intergenic
CAGATCTTACCAAATGACTT+TGG 0.384038 4:+56940763 MsG0480021350.01.T01:intron
TGCGAGTTATAGATGTAGTT+TGG 0.385150 4:-56940608 None:intergenic
CAATGAACTGGATGAACAAT+GGG 0.420513 4:+56940374 MsG0480021350.01.T01:CDS
ACACAAACTATTGTTAAGAT+AGG 0.424067 4:+56940886 MsG0480021350.01.T01:CDS
AATATTACAATACTTAAAAC+AGG 0.460074 4:-56941035 None:intergenic
TCCCAATGATTCCCAACAAA+AGG 0.467567 4:-56940466 None:intergenic
CATTCTCATTTACGATTGTG+TGG 0.506907 4:+56940808 MsG0480021350.01.T01:CDS
AGTGATGAAATAAGGTCTGT+TGG 0.517513 4:-56940213 None:intergenic
CATTATGAATGAAAGGAAGA+TGG 0.523264 4:-56940186 None:intergenic
TTGTTAAGATAGGACGTAGC+TGG 0.529092 4:+56940896 MsG0480021350.01.T01:CDS
GGTTAAATACAACAATGTGT+TGG 0.550805 4:-56940419 None:intergenic
TCAATGAACTGGATGAACAA+TGG 0.558244 4:+56940373 MsG0480021350.01.T01:CDS
GGCCATGAAACACGTGTATG+TGG 0.560867 4:-56940551 None:intergenic
TGGAGTCCATTATGAATGAA+AGG 0.569698 4:-56940193 None:intergenic
TCCAAACAAACGAATTTCCA+CGG 0.596587 4:+56940577 MsG0480021350.01.T01:CDS
GTAGTTTGGCTATGATACCG+TGG 0.697972 4:-56940594 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATATTATTTTTTAGCATAA+AGG - Chr4:56940926-56940945 None:intergenic 10.0%
!! CTAAATATGTAAAAGATATT+AGG - Chr4:56940518-56940537 None:intergenic 15.0%
!!! CTAATATCTTTTACATATTT+AGG + Chr4:56940516-56940535 MsG0480021350.01.T01:CDS 15.0%
!!! TTAAACTCTAAAATATCATT+TGG - Chr4:56940952-56940971 None:intergenic 15.0%
!! TACTATGAAATCAATGATTA+TGG + Chr4:56940492-56940511 MsG0480021350.01.T01:CDS 20.0%
!!! TATAAGGCATTGTTAAAATT+TGG - Chr4:56940443-56940462 None:intergenic 20.0%
! AAAAAAGATCCAAAGTCATT+TGG - Chr4:56940775-56940794 None:intergenic 25.0%
! ACACAAACTATTGTTAAGAT+AGG + Chr4:56940886-56940905 MsG0480021350.01.T01:CDS 25.0%
! ATGCCTAATATTGATGAATT+TGG + Chr4:56940651-56940670 MsG0480021350.01.T01:CDS 25.0%
! TGCCTAATATTGATGAATTT+GGG + Chr4:56940652-56940671 MsG0480021350.01.T01:CDS 25.0%
!! ACAACTGTCTTATATTTTTC+TGG + Chr4:56940694-56940713 MsG0480021350.01.T01:intron 25.0%
AAGAATTCCGCAATGAAAAT+TGG - Chr4:56940354-56940373 None:intergenic 30.0%
CACTGTATGCAAAAAAAATC+AGG + Chr4:56940232-56940251 MsG0480021350.01.T01:CDS 30.0%
CATCTTCCTTTCATTCATAA+TGG + Chr4:56940187-56940206 MsG0480021350.01.T01:CDS 30.0%
CATTATGAATGAAAGGAAGA+TGG - Chr4:56940189-56940208 None:intergenic 30.0%
GAAATTCGTTTGTTTGGATT+GGG - Chr4:56940575-56940594 None:intergenic 30.0%
GCATAAAAACCACAAAAGTT+GGG - Chr4:56940635-56940654 None:intergenic 30.0%
GGTTAAATACAACAATGTGT+TGG - Chr4:56940422-56940441 None:intergenic 30.0%
GTCCCAAATTCATCAATATT+AGG - Chr4:56940657-56940676 None:intergenic 30.0%
GTTTAAGTGTGACTCAATTA+TGG + Chr4:56940966-56940985 MsG0480021350.01.T01:CDS 30.0%
TCCCAACAAAAGGTAATATA+AGG - Chr4:56940459-56940478 None:intergenic 30.0%
TTGCATACAGTGATGAAATA+AGG - Chr4:56940224-56940243 None:intergenic 30.0%
! GCCTTATATTACCTTTTGTT+GGG + Chr4:56940455-56940474 MsG0480021350.01.T01:CDS 30.0%
! TGATGAATTTGGGACAAAAT+TGG + Chr4:56940662-56940681 MsG0480021350.01.T01:CDS 30.0%
! TGCCTTATATTACCTTTTGT+TGG + Chr4:56940454-56940473 MsG0480021350.01.T01:CDS 30.0%
!!! GACTTTGGATCTTTTTTGAA+AGG + Chr4:56940778-56940797 MsG0480021350.01.T01:CDS 30.0%
AGTGATGAAATAAGGTCTGT+TGG - Chr4:56940216-56940235 None:intergenic 35.0%
ATTTGGTAAGATCTGCAAGA+AGG - Chr4:56940758-56940777 None:intergenic 35.0%
CAATGAACTGGATGAACAAT+GGG + Chr4:56940374-56940393 MsG0480021350.01.T01:CDS 35.0%
CAGATCTTACCAAATGACTT+TGG + Chr4:56940763-56940782 MsG0480021350.01.T01:intron 35.0%
CATTCTCATTTACGATTGTG+TGG + Chr4:56940808-56940827 MsG0480021350.01.T01:CDS 35.0%
GATCTGCAAGAAGGAAAAAA+TGG - Chr4:56940749-56940768 None:intergenic 35.0%
GGAAATTCGTTTGTTTGGAT+TGG - Chr4:56940576-56940595 None:intergenic 35.0%
GGCATAAAAACCACAAAAGT+TGG - Chr4:56940636-56940655 None:intergenic 35.0%
TCAATGAACTGGATGAACAA+TGG + Chr4:56940373-56940392 MsG0480021350.01.T01:CDS 35.0%
TCCAAACAAACGAATTTCCA+CGG + Chr4:56940577-56940596 MsG0480021350.01.T01:CDS 35.0%
TGCGAGTTATAGATGTAGTT+TGG - Chr4:56940611-56940630 None:intergenic 35.0%
TGGAGTCCATTATGAATGAA+AGG - Chr4:56940196-56940215 None:intergenic 35.0%
! ACCTTTTGTTGGGAATCATT+GGG + Chr4:56940465-56940484 MsG0480021350.01.T01:CDS 35.0%
! GCTTGATCCAATTTTCATTG+CGG + Chr4:56940344-56940363 MsG0480021350.01.T01:CDS 35.0%
! TACCTTTTGTTGGGAATCAT+TGG + Chr4:56940464-56940483 MsG0480021350.01.T01:CDS 35.0%
ACCGTGGAAATTCGTTTGTT+TGG - Chr4:56940581-56940600 None:intergenic 40.0%
TCCCAATGATTCCCAACAAA+AGG - Chr4:56940469-56940488 None:intergenic 40.0%
TGCGGAATTCTTCAATGAAC+TGG + Chr4:56940362-56940381 MsG0480021350.01.T01:CDS 40.0%
TTCCACATACACGTGTTTCA+TGG + Chr4:56940549-56940568 MsG0480021350.01.T01:CDS 40.0%
TTGTTAAGATAGGACGTAGC+TGG + Chr4:56940896-56940915 MsG0480021350.01.T01:CDS 40.0%
GTAGTTTGGCTATGATACCG+TGG - Chr4:56940597-56940616 None:intergenic 45.0%
! AACTCGCAACCCAACTTTTG+TGG + Chr4:56940623-56940642 MsG0480021350.01.T01:CDS 45.0%
GATCAAGCTCAGCACTCATC+TGG - Chr4:56940332-56940351 None:intergenic 50.0%
GGCCATGAAACACGTGTATG+TGG - Chr4:56940554-56940573 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
Chr4 gene 56940167 56941044 56940167 ID=MsG0480021350.01;Name=MsG0480021350.01
Chr4 mRNA 56940167 56941044 56940167 ID=MsG0480021350.01.T01;Parent=MsG0480021350.01;Name=MsG0480021350.01.T01;_AED=0.50;_eAED=0.54;_QI=0|0|0|1|0.5|0.66|3|0|238
Chr4 exon 56940167 56940253 56940167 ID=MsG0480021350.01.T01:exon:7131;Parent=MsG0480021350.01.T01
Chr4 exon 56940333 56940683 56940333 ID=MsG0480021350.01.T01:exon:7132;Parent=MsG0480021350.01.T01
Chr4 exon 56940766 56941044 56940766 ID=MsG0480021350.01.T01:exon:7133;Parent=MsG0480021350.01.T01
Chr4 CDS 56940167 56940253 56940167 ID=MsG0480021350.01.T01:cds;Parent=MsG0480021350.01.T01
Chr4 CDS 56940333 56940683 56940333 ID=MsG0480021350.01.T01:cds;Parent=MsG0480021350.01.T01
Chr4 CDS 56940766 56941044 56940766 ID=MsG0480021350.01.T01:cds;Parent=MsG0480021350.01.T01
Gene Sequence

>MsG0480021350.01.T01

TTGCAACACAATTTCATCTCCATCTTCCTTTCATTCATAATGGACTCCAACAGACCTTATTTCATCACTGTATGCAAAAAAAATCAGATGAGTGCTGAGCTTGATCCAATTTTCATTGCGGAATTCTTCAATGAACTGGATGAACAATGGGTCTTGATTGATGAAAATGAGAACCAACACATTGTTGTATTTAACCAAATTTTAACAATGCCTTATATTACCTTTTGTTGGGAATCATTGGGAATTTACTATGAAATCAATGATTATGGCCTAATATCTTTTACATATTTAGGTGAAAGTAAGTTCCACATACACGTGTTTCATGGCCCAATCCAAACAAACGAATTTCCACGGTATCATAGCCAAACTACATCTATAACTCGCAACCCAACTTTTGTGGTTTTTATGCCTAATATTGATGAATTTGGGACAAAATTGATCTTACCAAATGACTTTGGATCTTTTTTGAAAGGAAAAAAACATTCTCATTTACGATTGTGTGGATCGTCAAATTCTATCACAACTTGCAAGTTGTACTTTGAAGATAATCAACAACAAACACAAACTATTGTTAAGATAGGACGTAGCTGGACTACCTTTATGCTAAAAAATAATATTTATCCAAATGATATTTTAGAGTTTAAGTGTGACTCAATTATGGCAACAAATATTGTCATTGTAATCAAGTATGATGAAAGTTCAAGACACCTGTTTTAA

Protein sequence

>MsG0480021350.01.T01

LQHNFISIFLSFIMDSNRPYFITVCKKNQMSAELDPIFIAEFFNELDEQWVLIDENENQHIVVFNQILTMPYITFCWESLGIYYEINDYGLISFTYLGESKFHIHVFHGPIQTNEFPRYHSQTTSITRNPTFVVFMPNIDEFGTKLILPNDFGSFLKGKKHSHLRLCGSSNSITTCKLYFEDNQQQTQTIVKIGRSWTTFMLKNNIYPNDILEFKCDSIMATNIVIVIKYDESSRHLF*