AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680033038.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680033038.01.T01 MTR_1g106600 86.577 149 13 1 1 142 79 227 3.48e-91 272
MsG0680033038.01.T01 MTR_1g106608 86.577 149 13 1 1 142 79 227 4.33e-91 271
MsG0680033038.01.T01 MTR_6g025650 85.906 149 14 1 1 142 79 227 1.58e-90 270
MsG0680033038.01.T01 MTR_6g018520 85.906 149 14 1 1 142 79 227 1.58e-90 270
MsG0680033038.01.T01 MTR_0002s1260 73.881 134 16 2 11 144 1 115 3.27e-47 153
MsG0680033038.01.T01 MTR_0002s1240 73.881 134 16 2 11 144 1 115 3.27e-47 153
MsG0680033038.01.T01 MTR_3g097540 85.556 90 13 0 55 144 1 90 2.93e-28 106
MsG0680033038.01.T01 MTR_7g111990 82.000 50 9 0 1 50 79 128 5.20e-22 88.6
MsG0680033038.01.T01 MTR_7g017480 62.069 58 19 1 43 100 24 78 5.29e-16 70.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680033038.01.T01 AT5G27260 41.573 89 51 1 1 89 74 161 2.88e-18 82.0
MsG0680033038.01.T01 AT2G29880 35.165 91 58 1 1 91 79 168 2.11e-13 68.6
MsG0680033038.01.T01 AT1G30140 28.571 147 98 3 1 147 70 209 4.30e-13 66.6
MsG0680033038.01.T01 AT1G30140 28.571 147 98 3 1 147 70 209 4.30e-13 66.6

Find 30 sgRNAs with CRISPR-Local

Find 244 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGCGTAACAACTCTGGTTT+TGG 0.286642 6:-55368901 MsG0680033038.01.T01:CDS
TGCCATCATATACATGTATA+TGG 0.330885 6:+55367756 None:intergenic
TACGGTGGTTCATAGTTATC+TGG 0.342012 6:+55368519 None:intergenic
TGCTATGAATGCATCACTAT+TGG 0.349730 6:+55368542 None:intergenic
GAGCAAGAAAGTGAAAGTTT+TGG 0.364343 6:-55368588 MsG0680033038.01.T01:CDS
AGTATGGAGTAATTACTTAA+AGG 0.368835 6:-55368839 MsG0680033038.01.T01:intron
GTTGGAGTTTGATTTGAATC+TGG 0.376903 6:-55365150 MsG0680033038.01.T01:CDS
GGTGGTGGAGTAGGTTGGTT+AGG 0.436796 6:+55368483 None:intergenic
GATTATTTGAAGATTCTTGT+TGG 0.439501 6:-55368678 MsG0680033038.01.T01:CDS
TTGAAGATTCTTGTTGGAGG+TGG 0.445118 6:-55368672 MsG0680033038.01.T01:CDS
GTTGGTTAGGTGATGGAGGT+TGG 0.445510 6:+55368496 None:intergenic
ACACGGCTACTGAAGAAGTA+TGG 0.447236 6:-55368855 MsG0680033038.01.T01:CDS
GAAGGAATGAGTGAAGATAA+AGG 0.460052 6:-55367847 MsG0680033038.01.T01:CDS
GCTATGAATGCATCACTATT+GGG 0.464417 6:+55368543 None:intergenic
AGTTTGATTTGAATCTGGAT+TGG 0.486656 6:-55365145 MsG0680033038.01.T01:CDS
ACGTTTATGCGTAACAACTC+TGG 0.489769 6:-55368907 MsG0680033038.01.T01:CDS
TTAGGTGATGGAGGTTGGTA+CGG 0.489944 6:+55368501 None:intergenic
AATGGGGTGGTGGAGTAGGT+TGG 0.496126 6:+55368478 None:intergenic
GTAGGTTGGTTAGGTGATGG+AGG 0.512588 6:+55368492 None:intergenic
GACGAAGAAATTGATTATGA+AGG 0.532361 6:-55367865 MsG0680033038.01.T01:CDS
GGAGTAGGTTGGTTAGGTGA+TGG 0.533493 6:+55368489 None:intergenic
AGCCATATACATGTATATGA+TGG 0.544261 6:-55367758 MsG0680033038.01.T01:CDS
TTGGAGGTGGAGTGTCTAGC+GGG 0.545573 6:-55368659 MsG0680033038.01.T01:CDS
ATTACTTGAGCCGAATGAAG+TGG 0.570458 6:-55368957 None:intergenic
TATTTGAAGATTCTTGTTGG+AGG 0.595361 6:-55368675 MsG0680033038.01.T01:CDS
TTCTATAGCATTAGACGCTG+AGG 0.600695 6:-55368631 MsG0680033038.01.T01:CDS
AATTCATGCTTTAATTCACG+TGG 0.601429 6:-55367734 MsG0680033038.01.T01:intron
GGTGATGGAGGTTGGTACGG+TGG 0.605354 6:+55368504 None:intergenic
GTTGGAGGTGGAGTGTCTAG+CGG 0.610018 6:-55368660 MsG0680033038.01.T01:CDS
TTCAGTTGCAAAGACTTACA+CGG 0.635359 6:-55368872 MsG0680033038.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTGATTAAGAAAATAAAA+AGG - Chr6:55367090-55367109 MsG0680033038.01.T01:intron 10.0%
!! TATATACATAAATCATTAAA+TGG - Chr6:55366451-55366470 MsG0680033038.01.T01:intron 10.0%
!!! TAAAGATGTAAAAATTTTAA+AGG + Chr6:55368521-55368540 None:intergenic 10.0%
!!! TTTTATTTTATTTGAATTGA+AGG - Chr6:55366851-55366870 MsG0680033038.01.T01:intron 10.0%
!! AAATAAAAAGGAAAATACAA+TGG - Chr6:55367102-55367121 MsG0680033038.01.T01:intron 15.0%
!! AAGAAAAAATAAGATATCAA+AGG + Chr6:55366621-55366640 None:intergenic 15.0%
!! AATAAAAAGGAAAATACAAT+GGG - Chr6:55367103-55367122 MsG0680033038.01.T01:intron 15.0%
!! TAAATATTGTCTTATAATCA+TGG - Chr6:55366178-55366197 MsG0680033038.01.T01:intron 15.0%
!! TATATATAAACAAAAACTGA+AGG - Chr6:55368344-55368363 MsG0680033038.01.T01:intron 15.0%
!! TGATTATAAGACAATATTTA+AGG + Chr6:55366178-55366197 None:intergenic 15.0%
!!! AAAAATGTTTTTGTTTTTGT+TGG - Chr6:55366935-55366954 MsG0680033038.01.T01:intron 15.0%
!!! ACAAAAACAAAAACATTTTT+TGG + Chr6:55366935-55366954 None:intergenic 15.0%
!!! TTGATATCTTATTTTTTCTT+GGG - Chr6:55366621-55366640 MsG0680033038.01.T01:intron 15.0%
!!! TTGATTTTAAGTTTGAAAAT+TGG - Chr6:55366958-55366977 MsG0680033038.01.T01:intron 15.0%
!!! TTTGATATCTTATTTTTTCT+TGG - Chr6:55366620-55366639 MsG0680033038.01.T01:intron 15.0%
!! AAAAATTTACTACAAGATGA+TGG - Chr6:55367642-55367661 MsG0680033038.01.T01:intron 20.0%
!! AACCTATAATTTCTACATTA+TGG - Chr6:55367674-55367693 MsG0680033038.01.T01:intron 20.0%
!! TTTATGGAACAAGAAATAAA+AGG - Chr6:55367520-55367539 MsG0680033038.01.T01:intron 20.0%
!!! AAAATCTTGCTATAATTTCT+TGG + Chr6:55367760-55367779 None:intergenic 20.0%
!!! AGAAATTATAGCAAGATTTT+AGG - Chr6:55367760-55367779 MsG0680033038.01.T01:CDS 20.0%
!!! ATTTTGTTGAACTATAGTTT+TGG - Chr6:55366020-55366039 MsG0680033038.01.T01:intron 20.0%
!!! GATGTAAAAATTTTAAAGGT+CGG + Chr6:55368517-55368536 None:intergenic 20.0%
!!! GTGATATTGTTCATATTTTT+TGG - Chr6:55367162-55367181 MsG0680033038.01.T01:intron 20.0%
!!! TAAATGTTGATGTGTTTTAA+AGG + Chr6:55368035-55368054 None:intergenic 20.0%
!!! TCATCTTGTAGTAAATTTTT+AGG + Chr6:55367642-55367661 None:intergenic 20.0%
!!! TGTACTTTTGTTAAAGTATT+TGG - Chr6:55366057-55366076 MsG0680033038.01.T01:intron 20.0%
!!! TTTTGTTGAACTATAGTTTT+GGG - Chr6:55366021-55366040 MsG0680033038.01.T01:intron 20.0%
!!! TTTTTTTTTTGAGTCTATTG+TGG - Chr6:55366088-55366107 MsG0680033038.01.T01:intron 20.0%
! AACATCAAGAATCATTATCA+AGG - Chr6:55367902-55367921 MsG0680033038.01.T01:intron 25.0%
! AAGACCATATAATGTAAACT+AGG + Chr6:55367364-55367383 None:intergenic 25.0%
! AATGAAGTCTAAATCAGATA+AGG - Chr6:55365895-55365914 MsG0680033038.01.T01:intron 25.0%
! ACTTAGTATGTAAAAAGCTT+AGG + Chr6:55367432-55367451 None:intergenic 25.0%
! AGACCATATAATGTAAACTA+GGG + Chr6:55367363-55367382 None:intergenic 25.0%
! AGAGCTGAAGAATAATATTT+GGG - Chr6:55365814-55365833 MsG0680033038.01.T01:intron 25.0%
! AGTATGGAGTAATTACTTAA+AGG - Chr6:55365240-55365259 MsG0680033038.01.T01:intron 25.0%
! CTCTGAATCTAAAGAAAAAT+GGG + Chr6:55365621-55365640 None:intergenic 25.0%
! GAAGATGTTGAAAAATCTTT+AGG - Chr6:55368069-55368088 MsG0680033038.01.T01:intron 25.0%
! GATTATTTGAAGATTCTTGT+TGG - Chr6:55365401-55365420 MsG0680033038.01.T01:intron 25.0%
! TAGAGCTGAAGAATAATATT+TGG - Chr6:55365813-55365832 MsG0680033038.01.T01:intron 25.0%
! TATAAGAGAAAAAACACCTT+CGG - Chr6:55366540-55366559 MsG0680033038.01.T01:intron 25.0%
! TCTGAATCTAAAGAAAAATG+GGG + Chr6:55365620-55365639 None:intergenic 25.0%
! TGCCATAATGTAGAAATTAT+AGG + Chr6:55367679-55367698 None:intergenic 25.0%
!! ACCATTTTCTAAATAAACCT+TGG + Chr6:55367138-55367157 None:intergenic 25.0%
!! CCATTTTCTAAATAAACCTT+GGG + Chr6:55367137-55367156 None:intergenic 25.0%
!! CTTGGATAAAATTCAAGTTA+GGG - Chr6:55365954-55365973 MsG0680033038.01.T01:intron 25.0%
!! GCATTTTTGTCAAAGTTTTT+AGG - Chr6:55368091-55368110 MsG0680033038.01.T01:intron 25.0%
!! TCCATTTGATTTCCATATTA+TGG + Chr6:55367728-55367747 None:intergenic 25.0%
!! TCTTGGATAAAATTCAAGTT+AGG - Chr6:55365953-55365972 MsG0680033038.01.T01:intron 25.0%
!! TTGGATAAAATTCAAGTTAG+GGG - Chr6:55365955-55365974 MsG0680033038.01.T01:intron 25.0%
!! TTTTTCTAAGTGTAAAACCT+AGG - Chr6:55367194-55367213 MsG0680033038.01.T01:intron 25.0%
!!! AATATTTTTGTGAGATGGAT+GGG + Chr6:55365357-55365376 None:intergenic 25.0%
!!! ATCTTATTTTTTCTTGGGAA+GGG - Chr6:55366626-55366645 MsG0680033038.01.T01:intron 25.0%
!!! GTTCATATTTTTTGGAGAAA+AGG - Chr6:55367170-55367189 MsG0680033038.01.T01:intron 25.0%
!!! TATCTTATTTTTTCTTGGGA+AGG - Chr6:55366625-55366644 MsG0680033038.01.T01:intron 25.0%
AAAACCTTTCTTCTAGCATT+GGG - Chr6:55365736-55365755 MsG0680033038.01.T01:intron 30.0%
AAAATAAAGACGGTTCCTAT+AGG + Chr6:55366739-55366758 None:intergenic 30.0%
AAATAAGCTTACTGACGAAA+AGG + Chr6:55368759-55368778 None:intergenic 30.0%
AATTCATGCTTTAATTCACG+TGG - Chr6:55366345-55366364 MsG0680033038.01.T01:intron 30.0%
ACATGGATACTTTAGATGTA+CGG + Chr6:55367272-55367291 None:intergenic 30.0%
AGAATTCACTACACAAATCA+AGG - Chr6:55367839-55367858 MsG0680033038.01.T01:CDS 30.0%
AGCCATATACATGTATATGA+TGG - Chr6:55366321-55366340 MsG0680033038.01.T01:intron 30.0%
ATAGGATACTATAGTCGTTA+TGG + Chr6:55366721-55366740 None:intergenic 30.0%
ATGCCCTAGTTTACATTATA+TGG - Chr6:55367357-55367376 MsG0680033038.01.T01:intron 30.0%
CCCAAGGTTTATTTAGAAAA+TGG - Chr6:55367134-55367153 MsG0680033038.01.T01:intron 30.0%
CCTCTGAATCTAAAGAAAAA+TGG + Chr6:55365622-55365641 None:intergenic 30.0%
GACGAAGAAATTGATTATGA+AGG - Chr6:55366214-55366233 MsG0680033038.01.T01:intron 30.0%
GATTTCCATATTATGGCTAA+AGG + Chr6:55367721-55367740 None:intergenic 30.0%
GCATGCATATAGCAAAAAAA+TGG - Chr6:55368143-55368162 MsG0680033038.01.T01:intron 30.0%
GCATGTTTAGATGTATGTTT+AGG + Chr6:55367240-55367259 None:intergenic 30.0%
GCCATAATATGGAAATCAAA+TGG - Chr6:55367724-55367743 MsG0680033038.01.T01:intron 30.0%
GGATGTCTATGAAATGTTTA+TGG - Chr6:55367504-55367523 MsG0680033038.01.T01:intron 30.0%
GTGAAAACATTGTGTTCTTT+GGG + Chr6:55367992-55368011 None:intergenic 30.0%
TAACGACTATAGTATCCTAT+AGG - Chr6:55366721-55366740 MsG0680033038.01.T01:intron 30.0%
TACAATCTTGTAACAAACTC+AGG - Chr6:55368857-55368876 MsG0680033038.01.T01:CDS 30.0%
TAGGATACTATAGTCGTTAT+GGG + Chr6:55366720-55366739 None:intergenic 30.0%
TATTTGAAGATTCTTGTTGG+AGG - Chr6:55365404-55365423 MsG0680033038.01.T01:intron 30.0%
TGAAAAGATGTCTAACATGA+TGG - Chr6:55365776-55365795 MsG0680033038.01.T01:intron 30.0%
TGCCATCATATACATGTATA+TGG + Chr6:55366326-55366345 None:intergenic 30.0%
TTCCTAAGTGAAGAATTAGA+GGG - Chr6:55366810-55366829 MsG0680033038.01.T01:intron 30.0%
TTCTTCCTTTAGCCATAATA+TGG - Chr6:55367713-55367732 MsG0680033038.01.T01:intron 30.0%
TTGATTCAGTGTATGTGAAA+AGG + Chr6:55368643-55368662 None:intergenic 30.0%
TTTCCTAAGTGAAGAATTAG+AGG - Chr6:55366809-55366828 MsG0680033038.01.T01:intron 30.0%
! AGTTGTTTGTTTAGAGTCTT+TGG + Chr6:55367589-55367608 None:intergenic 30.0%
! ATGACGATTTCGTTTTCAAT+AGG + Chr6:55365647-55365666 None:intergenic 30.0%
! ATGTCTAACATGATGGAAAA+GGG - Chr6:55365783-55365802 MsG0680033038.01.T01:intron 30.0%
! CAGTAAGCTTATTTGTTTCT+TGG - Chr6:55368765-55368784 MsG0680033038.01.T01:intron 30.0%
! CAGTTTTTGAATATGATGTG+TGG - Chr6:55366373-55366392 MsG0680033038.01.T01:intron 30.0%
! GCTTTTTACATACTAAGTCT+TGG - Chr6:55367434-55367453 MsG0680033038.01.T01:intron 30.0%
! TAAGATATCAAAGGTGCAAA+AGG + Chr6:55366612-55366631 None:intergenic 30.0%
! TGAATTTTATCCAAGACAAG+CGG + Chr6:55365949-55365968 None:intergenic 30.0%
! TGATTTGTGTAGTGAATTCT+TGG + Chr6:55367839-55367858 None:intergenic 30.0%
!! AAAAATTTTAAAGGTCGGAG+TGG + Chr6:55368512-55368531 None:intergenic 30.0%
!! AGTTTGATTTGAATCTGGAT+TGG - Chr6:55368934-55368953 MsG0680033038.01.T01:CDS 30.0%
!! CCATTTTTCTTTAGATTCAG+AGG - Chr6:55365619-55365638 MsG0680033038.01.T01:intron 30.0%
!! CGAGTTTTGTCATCTAAATT+TGG + Chr6:55365857-55365876 None:intergenic 30.0%
!! GAATATTTTTGTGAGATGGA+TGG + Chr6:55365358-55365377 None:intergenic 30.0%
!! GGTTGTTTTATCTTGGTTTT+CGG - Chr6:55366561-55366580 MsG0680033038.01.T01:intron 30.0%
!! GTTGTTTTATCTTGGTTTTC+GGG - Chr6:55366562-55366581 MsG0680033038.01.T01:intron 30.0%
!! TGCTGAGATTTTGTTTGATT+CGG - Chr6:55368905-55368924 MsG0680033038.01.T01:CDS 30.0%
!! TTCAGAAAAAGTGTTCGAAT+CGG - Chr6:55368581-55368600 MsG0680033038.01.T01:CDS 30.0%
!!! AGAAAGTGAAAGTTTTGGAA+TGG - Chr6:55365496-55365515 MsG0680033038.01.T01:intron 30.0%
!!! CAAAGTTTTTAGGTAAGCAA+TGG - Chr6:55368101-55368120 MsG0680033038.01.T01:intron 30.0%
!!! CATTTTTTGGAAGAGAACTA+CGG + Chr6:55366922-55366941 None:intergenic 30.0%
AACCAAGATAAAACAACCGA+AGG + Chr6:55366559-55366578 None:intergenic 35.0%
AACTGAAGGAATACACACAA+AGG - Chr6:55368358-55368377 MsG0680033038.01.T01:intron 35.0%
AATCAAACGATTTACGATGG+AGG - Chr6:55368657-55368676 MsG0680033038.01.T01:CDS 35.0%
AGATGAATGTGAGATGAGAT+AGG - Chr6:55367482-55367501 MsG0680033038.01.T01:intron 35.0%
ATTTGAAGAATGAGAGAGAG+AGG - Chr6:55366982-55367001 MsG0680033038.01.T01:intron 35.0%
CATAACTTTCGAAAGACATC+AGG + Chr6:55366514-55366533 None:intergenic 35.0%
CCATCTCACAAAAATATTCG+AGG - Chr6:55365359-55365378 MsG0680033038.01.T01:intron 35.0%
CGTGAAAACATTGTGTTCTT+TGG + Chr6:55367993-55368012 None:intergenic 35.0%
CTGAATCAAACGATTTACGA+TGG - Chr6:55368654-55368673 MsG0680033038.01.T01:CDS 35.0%
CTTTGGGTGATTCAATACTT+TGG + Chr6:55367976-55367995 None:intergenic 35.0%
GAAAACCTTTCTTCTAGCAT+TGG - Chr6:55365735-55365754 MsG0680033038.01.T01:intron 35.0%
GAAGGAATGAGTGAAGATAA+AGG - Chr6:55366232-55366251 MsG0680033038.01.T01:intron 35.0%
GAATCTAAAGAAAAATGGGG+TGG + Chr6:55365617-55365636 None:intergenic 35.0%
GAATGTTTAGATGAATGCCT+AGG + Chr6:55367214-55367233 None:intergenic 35.0%
GATGAATGTGAGATGAGATA+GGG - Chr6:55367483-55367502 MsG0680033038.01.T01:intron 35.0%
GCTATGAATGCATCACTATT+GGG + Chr6:55365539-55365558 None:intergenic 35.0%
GTGGATTTGATCCTTTAAAC+GGG + Chr6:55366900-55366919 None:intergenic 35.0%
TAACTTGAAGTTCGAAGAAC+CGG + Chr6:55368826-55368845 None:intergenic 35.0%
TAATCATGGCAATGACATAG+AGG - Chr6:55366192-55366211 MsG0680033038.01.T01:intron 35.0%
TCTTCACTTAGGAAACTCTT+AGG + Chr6:55366804-55366823 None:intergenic 35.0%
TGCTATGAATGCATCACTAT+TGG + Chr6:55365540-55365559 None:intergenic 35.0%
TTCACAATCTTATGCCCTTA+AGG + Chr6:55367008-55367027 None:intergenic 35.0%
TTCAGTTGCAAAGACTTACA+CGG - Chr6:55365207-55365226 MsG0680033038.01.T01:intron 35.0%
! AACATGATGGAAAAGGGAAA+AGG - Chr6:55365789-55365808 MsG0680033038.01.T01:intron 35.0%
! GAGCAAGAAAGTGAAAGTTT+TGG - Chr6:55365491-55365510 MsG0680033038.01.T01:intron 35.0%
! GATGTCTAACATGATGGAAA+AGG - Chr6:55365782-55365801 MsG0680033038.01.T01:intron 35.0%
! GGTGGATTTGATCCTTTAAA+CGG + Chr6:55366901-55366920 None:intergenic 35.0%
!! CCTCGAATATTTTTGTGAGA+TGG + Chr6:55365362-55365381 None:intergenic 35.0%
!! GTTGGAGTTTGATTTGAATC+TGG - Chr6:55368929-55368948 MsG0680033038.01.T01:CDS 35.0%
!!! AGTGAAAGTTTTGGAATGGA+AGG - Chr6:55365500-55365519 MsG0680033038.01.T01:intron 35.0%
!!! GATTTTGTTTGATTCGGTGT+TGG - Chr6:55368911-55368930 MsG0680033038.01.T01:CDS 35.0%
!!! GTGAAAGTTTTGGAATGGAA+GGG - Chr6:55365501-55365520 MsG0680033038.01.T01:intron 35.0%
!!! TAACAACTCTGGTTTTGGAT+GGG - Chr6:55365183-55365202 MsG0680033038.01.T01:CDS 35.0%
ACGTTTATGCGTAACAACTC+TGG - Chr6:55365172-55365191 MsG0680033038.01.T01:CDS 40.0%
AGAAATAGATCCGAGTACGA+AGG - Chr6:55365670-55365689 MsG0680033038.01.T01:intron 40.0%
ATCTAACGGTAGCAAAGCAA+AGG - Chr6:55368276-55368295 MsG0680033038.01.T01:intron 40.0%
ATGCGTGTTTCTCTCTCTAA+AGG - Chr6:55368383-55368402 MsG0680033038.01.T01:intron 40.0%
CCCTAAAACCCTAGATCTAA+CGG - Chr6:55368262-55368281 MsG0680033038.01.T01:intron 40.0%
CGACGACGAATTACAAGTAA+GGG - Chr6:55366421-55366440 MsG0680033038.01.T01:intron 40.0%
CTCCCTCTAATTCTTCACTT+AGG + Chr6:55366815-55366834 None:intergenic 40.0%
CTTTGCTACCGTTAGATCTA+GGG + Chr6:55368273-55368292 None:intergenic 40.0%
GATACTTTAGATGTACGGCT+AGG + Chr6:55367267-55367286 None:intergenic 40.0%
GCGAAGAAGAGAAAGAAGAA+AGG + Chr6:55368794-55368813 None:intergenic 40.0%
GGGCATGATGTAAAAGACAA+TGG + Chr6:55367343-55367362 None:intergenic 40.0%
GTAATTCGTCGTCGAGTTAA+TGG + Chr6:55366415-55366434 None:intergenic 40.0%
GTCACATACCGTGTTCAAAT+CGG - Chr6:55368692-55368711 MsG0680033038.01.T01:CDS 40.0%
GTTGTTGGGATCAACAATCA+AGG - Chr6:55365702-55365721 MsG0680033038.01.T01:intron 40.0%
TACGGTGGTTCATAGTTATC+TGG + Chr6:55365563-55365582 None:intergenic 40.0%
TAGTCCCAATGCTAGAAGAA+AGG + Chr6:55365743-55365762 None:intergenic 40.0%
TCGACGACGAATTACAAGTA+AGG - Chr6:55366420-55366439 MsG0680033038.01.T01:intron 40.0%
TCTAAAGAAAAATGGGGTGG+TGG + Chr6:55365614-55365633 None:intergenic 40.0%
TTCTATAGCATTAGACGCTG+AGG - Chr6:55365448-55365467 MsG0680033038.01.T01:intron 40.0%
TTGAAGATTCTTGTTGGAGG+TGG - Chr6:55365407-55365426 MsG0680033038.01.T01:intron 40.0%
TTTAGAGAGAGAAACACGCA+TGG + Chr6:55368384-55368403 None:intergenic 40.0%
! GAAGAAAGGTTTTCCATAGC+AGG + Chr6:55365729-55365748 None:intergenic 40.0%
! GAAGGAGTTCTAGTGTTGTT+GGG - Chr6:55365688-55365707 MsG0680033038.01.T01:intron 40.0%
!! ACCGTTAGATCTAGGGTTTT+AGG + Chr6:55368266-55368285 None:intergenic 40.0%
!! ATGCGTAACAACTCTGGTTT+TGG - Chr6:55365178-55365197 MsG0680033038.01.T01:CDS 40.0%
!! CACCTTCGGTTGTTTTATCT+TGG - Chr6:55366554-55366573 MsG0680033038.01.T01:intron 40.0%
!! CCGTTAGATCTAGGGTTTTA+GGG + Chr6:55368265-55368284 None:intergenic 40.0%
!! CGTTAGATCTAGGGTTTTAG+GGG + Chr6:55368264-55368283 None:intergenic 40.0%
!! TTTTAAAGGTCGGAGTGGAA+CGG + Chr6:55368507-55368526 None:intergenic 40.0%
!! TTTTTGGAAGAGAACTACGG+TGG + Chr6:55366919-55366938 None:intergenic 40.0%
!!! ATCTAGGGTTTTAGGGGATT+CGG + Chr6:55368258-55368277 None:intergenic 40.0%
!!! GTAACAACTCTGGTTTTGGA+TGG - Chr6:55365182-55365201 MsG0680033038.01.T01:CDS 40.0%
AACTAGAGAGAGAGTCTGCA+AGG - Chr6:55368410-55368429 MsG0680033038.01.T01:intron 45.0%
AATGAGAGAGAGAGGCCTTA+AGG - Chr6:55366990-55367009 MsG0680033038.01.T01:intron 45.0%
ACAATGGGATTGTGATCCCA+AGG - Chr6:55367118-55367137 MsG0680033038.01.T01:intron 45.0%
ACACATGCACACACATCACA+TGG + Chr6:55367289-55367308 None:intergenic 45.0%
ACACGGCTACTGAAGAAGTA+TGG - Chr6:55365224-55365243 MsG0680033038.01.T01:intron 45.0%
ACATACCGTGTTCAAATCGG+AGG - Chr6:55368695-55368714 MsG0680033038.01.T01:CDS 45.0%
AGCAAAACCCTTGAGAATCG+AGG - Chr6:55366657-55366676 MsG0680033038.01.T01:intron 45.0%
ATCCAGATCCAACCTGTGTT+TGG + Chr6:55368204-55368223 None:intergenic 45.0%
ATGAGAGAGAGAGGCCTTAA+GGG - Chr6:55366991-55367010 MsG0680033038.01.T01:intron 45.0%
CAACAATCAAGGACCTGCTA+TGG - Chr6:55365713-55365732 MsG0680033038.01.T01:intron 45.0%
CACTAGAACTCCTTCGTACT+CGG + Chr6:55365683-55365702 None:intergenic 45.0%
CCTCTTCTTGAAGATGTCAC+CGG - Chr6:55365919-55365938 MsG0680033038.01.T01:intron 45.0%
GATCCTTTAAACGGGTACGT+AGG + Chr6:55366892-55366911 None:intergenic 45.0%
GCGCGCGTAGAAAATAAAGA+CGG + Chr6:55366749-55366768 None:intergenic 45.0%
GCTTTGCTACCGTTAGATCT+AGG + Chr6:55368274-55368293 None:intergenic 45.0%
GTGTTCGAATCGGAGTAGAT+CGG - Chr6:55368591-55368610 MsG0680033038.01.T01:CDS 45.0%
TCCAGATCCAACCTGTGTTT+GGG + Chr6:55368203-55368222 None:intergenic 45.0%
TCCAGTGAAGAAGTGAATCC+AGG - Chr6:55368177-55368196 MsG0680033038.01.T01:intron 45.0%
TGTGATGTGTGTGCATGTGT+CGG - Chr6:55367289-55367308 MsG0680033038.01.T01:intron 45.0%
TTGCCTACGTACCCGTTTAA+AGG - Chr6:55366886-55366905 MsG0680033038.01.T01:intron 45.0%
TTTAAACGGGTACGTAGGCA+AGG + Chr6:55366887-55366906 None:intergenic 45.0%
! CCAAACCTCCGATTTGAACA+CGG + Chr6:55368703-55368722 None:intergenic 45.0%
! CGAAGGAGTTCTAGTGTTGT+TGG - Chr6:55365687-55365706 MsG0680033038.01.T01:intron 45.0%
! GTAGCAAAGCAAAGGAGCAT+GGG - Chr6:55368284-55368303 MsG0680033038.01.T01:intron 45.0%
!! TTAGGTGATGGAGGTTGGTA+CGG + Chr6:55365581-55365600 None:intergenic 45.0%
AACCCTTGAGAATCGAGGTC+CGG - Chr6:55366662-55366681 MsG0680033038.01.T01:intron 50.0%
AAGTTCGAAGAACCGGCACA+AGG + Chr6:55368819-55368838 None:intergenic 50.0%
AGGTTTGGCGAAGGTGAAGA+CGG - Chr6:55368715-55368734 MsG0680033038.01.T01:CDS 50.0%
AGTTCGAAGAACCGGCACAA+GGG + Chr6:55368818-55368837 None:intergenic 50.0%
ATCCAAGACAAGCGGTCTTC+CGG + Chr6:55365941-55365960 None:intergenic 50.0%
CCGGTGACATCTTCAAGAAG+AGG + Chr6:55365922-55365941 None:intergenic 50.0%
CCGTGTTCAAATCGGAGGTT+TGG - Chr6:55368700-55368719 MsG0680033038.01.T01:CDS 50.0%
GCCTGGATTCACTTCTTCAC+TGG + Chr6:55368181-55368200 None:intergenic 50.0%
GGTAGCAAAGCAAAGGAGCA+TGG - Chr6:55368283-55368302 MsG0680033038.01.T01:intron 50.0%
TCAAATCGGAGGTTTGGCGA+AGG - Chr6:55368706-55368725 MsG0680033038.01.T01:CDS 50.0%
TCTGGATCATCCAACAGCCT+TGG - Chr6:55368217-55368236 MsG0680033038.01.T01:intron 50.0%
!! GAAAAATGGGGTGGTGGAGT+AGG + Chr6:55365608-55365627 None:intergenic 50.0%
!! GAGTTGTTTTCGCTACGGGA+AGG - Chr6:55366685-55366704 MsG0680033038.01.T01:intron 50.0%
!! GGAGTAGGTTGGTTAGGTGA+TGG + Chr6:55365593-55365612 None:intergenic 50.0%
!! GTAGGTTGGTTAGGTGATGG+AGG + Chr6:55365590-55365609 None:intergenic 50.0%
!! GTTGGTTAGGTGATGGAGGT+TGG + Chr6:55365586-55365605 None:intergenic 50.0%
!! TCCGGGAGTTGTTTTCGCTA+CGG - Chr6:55366680-55366699 MsG0680033038.01.T01:intron 50.0%
ACCCTTGAGAATCGAGGTCC+GGG - Chr6:55366663-55366682 MsG0680033038.01.T01:intron 55.0%
ATCCAACCTGTGTTTGGGCC+TGG + Chr6:55368198-55368217 None:intergenic 55.0%
ATCCAGGCCCAAACACAGGT+TGG - Chr6:55368193-55368212 MsG0680033038.01.T01:intron 55.0%
CCCGTAGCGAAAACAACTCC+CGG + Chr6:55366684-55366703 None:intergenic 55.0%
GGATCAATCCAACGCCTCCA+AGG + Chr6:55368237-55368256 None:intergenic 55.0%
GGATCATCCAACAGCCTTGG+AGG - Chr6:55368220-55368239 MsG0680033038.01.T01:intron 55.0%
GTGAATCCAGGCCCAAACAC+AGG - Chr6:55368189-55368208 MsG0680033038.01.T01:intron 55.0%
TCCCGGACCTCGATTCTCAA+GGG + Chr6:55366667-55366686 None:intergenic 55.0%
TCTTCTTCGCTCCCTTGTGC+CGG - Chr6:55368804-55368823 MsG0680033038.01.T01:intron 55.0%
! GCATGTGTCGGTGCTTGTGT+CGG - Chr6:55367301-55367320 MsG0680033038.01.T01:intron 55.0%
! GCCCAAACACAGGTTGGATC+TGG - Chr6:55368199-55368218 MsG0680033038.01.T01:intron 55.0%
! GTTGGAGGTGGAGTGTCTAG+CGG - Chr6:55365419-55365438 MsG0680033038.01.T01:intron 55.0%
! TTGGAGGTGGAGTGTCTAGC+GGG - Chr6:55365420-55365439 MsG0680033038.01.T01:intron 55.0%
!! AATGGGGTGGTGGAGTAGGT+TGG + Chr6:55365604-55365623 None:intergenic 55.0%
!! CCGGGAGTTGTTTTCGCTAC+GGG - Chr6:55366681-55366700 MsG0680033038.01.T01:intron 55.0%
!! GGTGGTGGAGTAGGTTGGTT+AGG + Chr6:55365599-55365618 None:intergenic 55.0%
!! TCCGACGAAGCTTTGAGTGC+CGG - Chr6:55368443-55368462 MsG0680033038.01.T01:CDS 55.0%
CACCGGAAGACCGCTTGTCT+TGG - Chr6:55365936-55365955 MsG0680033038.01.T01:intron 60.0%
CTCCCGGACCTCGATTCTCA+AGG + Chr6:55366668-55366687 None:intergenic 60.0%
GCCGGCACTCAAAGCTTCGT+CGG + Chr6:55368447-55368466 None:intergenic 60.0%
TACACGCGCGCACGTGTGAT+TGG - Chr6:55368314-55368333 MsG0680033038.01.T01:intron 60.0%
TCCAACGCCTCCAAGGCTGT+TGG + Chr6:55368230-55368249 None:intergenic 60.0%
! CGGTTTCCGGTGACGGTTTC+CGG - Chr6:55368469-55368488 MsG0680033038.01.T01:CDS 60.0%
! TCCAACAGCCTTGGAGGCGT+TGG - Chr6:55368226-55368245 MsG0680033038.01.T01:intron 60.0%
!! GAAGCTTTGAGTGCCGGCGA+CGG - Chr6:55368449-55368468 MsG0680033038.01.T01:CDS 60.0%
!! GGTGATGGAGGTTGGTACGG+TGG + Chr6:55365578-55365597 None:intergenic 60.0%
CGGAGTGGAACGGAGTTCGC+CGG + Chr6:55368497-55368516 None:intergenic 65.0%
GCGCGCACGTGTGATTGGCA+AGG - Chr6:55368319-55368338 MsG0680033038.01.T01:intron 65.0%
!! TGAGTGCCGGCGACGGTTTC+CGG - Chr6:55368456-55368475 MsG0680033038.01.T01:CDS 65.0%
CCGGCGACGGTTTCCGGTGA+CGG - Chr6:55368462-55368481 MsG0680033038.01.T01:CDS 70.0%
CCGTCACCGGAAACCGTCGC+CGG + Chr6:55368465-55368484 None:intergenic 70.0%
GGAACGGAGTTCGCCGGCGC+CGG + Chr6:55368491-55368510 None:intergenic 75.0%
! CCGGCGCCGGAAACCGTCAC+CGG + Chr6:55368478-55368497 None:intergenic 75.0%
! CCGGTGACGGTTTCCGGCGC+CGG - Chr6:55368475-55368494 MsG0680033038.01.T01:CDS 75.0%
Chromosome Type Strat End Strand Name
Chr6 gene 55365136 55368965 55365136 ID=MsG0680033038.01;Name=MsG0680033038.01
Chr6 mRNA 55365136 55368965 55365136 ID=MsG0680033038.01.T01;Parent=MsG0680033038.01;Name=MsG0680033038.01.T01;_AED=0.45;_eAED=0.45;_QI=0|0|0|0.5|0.66|1|4|0|232
Chr6 exon 55368840 55368965 55368840 ID=MsG0680033038.01.T01:exon:12645;Parent=MsG0680033038.01.T01
Chr6 exon 55368418 55368748 55368418 ID=MsG0680033038.01.T01:exon:12646;Parent=MsG0680033038.01.T01
Chr6 exon 55367735 55367889 55367735 ID=MsG0680033038.01.T01:exon:12647;Parent=MsG0680033038.01.T01
Chr6 exon 55365136 55365222 55365136 ID=MsG0680033038.01.T01:exon:12648;Parent=MsG0680033038.01.T01
Chr6 CDS 55368840 55368965 55368840 ID=MsG0680033038.01.T01:cds;Parent=MsG0680033038.01.T01
Chr6 CDS 55368418 55368748 55368418 ID=MsG0680033038.01.T01:cds;Parent=MsG0680033038.01.T01
Chr6 CDS 55367735 55367889 55367735 ID=MsG0680033038.01.T01:cds;Parent=MsG0680033038.01.T01
Chr6 CDS 55365136 55365222 55365136 ID=MsG0680033038.01.T01:cds;Parent=MsG0680033038.01.T01
Gene Sequence

>MsG0680033038.01.T01

ATGAAGTGGTTTAGAACTCAATACAACAAGATGACAACGTTTATGCGTAACAACTCTGGTTTTGGATGGGATTCAGTTGCAAAGACTTACACGGCTACTGAAGAAGTATGGAGTAATTACTTAAAGTCCCATCCATCTCACAAAAATATTCGAGGAAAAAGTATGATTGATTATGATTATTTGAAGATTCTTGTTGGAGGTGGAGTGTCTAGCGGGAATAATTCTATAGCATTAGACGCTGAGGATATTGATGCAACAACTTTTGAGCAAGAAAGTGAAAGTTTTGGAATGGAAGGGTTTTCGTATGATCCCAATAGTGATGCATTCATAGCACCAGATAACTATGAACCACCGTACCAACCTCCATCACCTAACCAACCTACTCCACCACCCCATTTTTCTTTAGATTCAGAGGCTCCTATTGAAAACGAAATCGTCATAAGAGAAATAGATCCGAAGGACGAAGAAATTGATTATGAAGGAATGAGTGAAGATAAAGGTACTGAAGCTATAGATGATGATGATGATGATACAAATGATGAAGAAGCAGATGAAGAATTTTATGAAGCCATATACATGTATATGATGGCAATTCATGCTTTAATTCACGTGGTCGTAGCTTGTGATTCGAATTTGTGTGCTGAGATTTTGTTTGATTCGGTGTTGGAGTTTGATTTGAATCTGGATTGGCTTTCTTGA
Protein sequence

>MsG0680033038.01.T01

MKWFRTQYNKMTTFMRNNSGFGWDSVAKTYTATEEVWSNYLKSHPSHKNIRGKSMIDYDYLKILVGGGVSSGNNSIALDAEDIDATTFEQESESFGMEGFSYDPNSDAFIAPDNYEPPYQPPSPNQPTPPPHFSLDSEAPIENEIVIREIDPKDEEIDYEGMSEDKGTEAIDDDDDDTNDEEADEEFYEAIYMYMMAIHALIHVVVACDSNLCAEILFDSVLEFDLNLDWLS*