Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036378.01.T01 | XP_003621340.1 | 93.66 | 347 | 22 | 0 | 1 | 347 | 29 | 375 | 0 | 672 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036378.01.T01 | P93324 | 59.77 | 348 | 135 | 3 | 1 | 347 | 29 | 372 | 9.17E-151 | 432 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036378.01.T01 | G7L368 | 93.660 | 347 | 22 | 0 | 1 | 347 | 29 | 375 | 0.0 | 672 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0680032932.01 | MsG0780036378.01 | 0.806803 | 6.888595e-50 | 3.774099e-47 |
| MsG0680034238.01 | MsG0780036378.01 | 0.813645 | 2.305357e-51 | 1.513973e-48 |
| MsG0480019146.01 | MsG0780036378.01 | 0.850222 | 1.952288e-60 | 3.797491e-57 |
| MsG0480019842.01 | MsG0780036378.01 | 0.804160 | 2.468822e-49 | 1.263865e-46 |
| MsG0480020904.01 | MsG0780036378.01 | 0.825448 | 4.660758e-54 | 4.249128e-51 |
| MsG0480021360.01 | MsG0780036378.01 | 0.808161 | 3.547491e-50 | 2.013754e-47 |
| MsG0480021573.01 | MsG0780036378.01 | 0.800871 | 1.176202e-48 | 5.537283e-46 |
| MsG0580024087.01 | MsG0780036378.01 | 0.809161 | 2.169390e-50 | 1.264372e-47 |
| MsG0580024220.01 | MsG0780036378.01 | 0.825210 | 5.308596e-54 | 4.807547e-51 |
| MsG0380015660.01 | MsG0780036378.01 | 0.821871 | 3.206557e-53 | 2.640873e-50 |
| MsG0380016299.01 | MsG0780036378.01 | 0.825535 | 4.447058e-54 | 4.064490e-51 |
| MsG0380016300.01 | MsG0780036378.01 | 0.808537 | 2.949451e-50 | 1.690806e-47 |
| MsG0380017168.01 | MsG0780036378.01 | 0.823162 | 1.606551e-53 | 1.372568e-50 |
| MsG0380017901.01 | MsG0780036378.01 | 0.802650 | 5.073950e-49 | 2.498843e-46 |
| MsG0580026521.01 | MsG0780036378.01 | 0.809133 | 2.199287e-50 | 1.280860e-47 |
| MsG0580029676.01 | MsG0780036378.01 | 0.801612 | 8.292198e-49 | 3.977400e-46 |
| MsG0580029937.01 | MsG0780036378.01 | 0.802182 | 6.335318e-49 | 3.083075e-46 |
| MsG0780036378.01 | MsG0780036839.01 | 0.806536 | 7.845154e-50 | 4.268781e-47 |
| MsG0780036378.01 | MsG0780037998.01 | 0.802926 | 4.449486e-49 | 2.206839e-46 |
| MsG0780036378.01 | MsG0780038519.01 | 0.804564 | 2.032997e-49 | 1.051621e-46 |
| MsG0780036378.01 | MsG0780038657.01 | 0.820565 | 6.413933e-53 | 5.092745e-50 |
| MsG0780036378.01 | MsG0780039287.01 | -0.801484 | 8.810109e-49 | 4.212305e-46 |
| MsG0780036378.01 | MsG0780040498.01 | 0.812383 | 4.358401e-51 | 2.767108e-48 |
| MsG0780036378.01 | MsG0780041248.01 | 0.801895 | 7.255347e-49 | 3.505392e-46 |
| MsG0780036378.01 | MsG0780041317.01 | 0.804503 | 2.094045e-49 | 1.081448e-46 |
| MsG0780036378.01 | MsG0880043217.01 | 0.808935 | 2.425689e-50 | 1.405090e-47 |
| MsG0780036378.01 | MsG0880044712.01 | 0.800792 | 1.220239e-48 | 5.732877e-46 |
| MsG0780036378.01 | MsG0880045801.01 | 0.817028 | 4.077254e-52 | 2.936222e-49 |
| MsG0780036378.01 | MsG0880046870.01 | 0.800566 | 1.357425e-48 | 6.340957e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036378.01.T01 | MTR_7g012070 | 93.660 | 347 | 22 | 0 | 1 | 347 | 29 | 375 | 0.0 | 672 |
| MsG0780036378.01.T01 | MTR_7g012090 | 92.219 | 347 | 27 | 0 | 1 | 347 | 29 | 375 | 0.0 | 659 |
| MsG0780036378.01.T01 | MTR_7g012040 | 75.793 | 347 | 78 | 3 | 1 | 347 | 28 | 368 | 0.0 | 535 |
| MsG0780036378.01.T01 | MTR_7g012020 | 70.893 | 347 | 96 | 2 | 1 | 347 | 28 | 369 | 0.0 | 515 |
| MsG0780036378.01.T01 | MTR_7g011900 | 70.605 | 347 | 97 | 2 | 1 | 347 | 29 | 370 | 0.0 | 506 |
| MsG0780036378.01.T01 | MTR_7g011990 | 66.954 | 348 | 105 | 3 | 1 | 347 | 29 | 367 | 5.90e-170 | 478 |
| MsG0780036378.01.T01 | MTR_2g055940 | 63.401 | 347 | 121 | 2 | 1 | 347 | 23 | 363 | 2.49e-162 | 458 |
| MsG0780036378.01.T01 | MTR_1g096310 | 60.057 | 348 | 134 | 3 | 1 | 347 | 29 | 372 | 1.55e-152 | 434 |
| MsG0780036378.01.T01 | MTR_3g021440 | 55.620 | 347 | 147 | 3 | 1 | 347 | 31 | 370 | 1.76e-130 | 377 |
| MsG0780036378.01.T01 | MTR_3g021430 | 56.305 | 341 | 142 | 3 | 7 | 347 | 24 | 357 | 1.84e-130 | 377 |
| MsG0780036378.01.T01 | MTR_7g012020 | 71.318 | 258 | 68 | 3 | 1 | 257 | 28 | 280 | 1.17e-129 | 373 |
| MsG0780036378.01.T01 | MTR_1g036460 | 51.003 | 349 | 165 | 5 | 1 | 347 | 26 | 370 | 5.98e-123 | 358 |
| MsG0780036378.01.T01 | MTR_1g036490 | 51.289 | 349 | 164 | 5 | 1 | 347 | 26 | 370 | 5.29e-122 | 356 |
| MsG0780036378.01.T01 | MTR_1g036510 | 53.009 | 349 | 158 | 5 | 1 | 347 | 40 | 384 | 8.24e-120 | 351 |
| MsG0780036378.01.T01 | MTR_7g012020 | 72.174 | 230 | 59 | 2 | 1 | 230 | 28 | 252 | 8.61e-117 | 338 |
| MsG0780036378.01.T01 | MTR_3g092900 | 45.559 | 349 | 179 | 7 | 1 | 347 | 24 | 363 | 6.06e-97 | 292 |
| MsG0780036378.01.T01 | MTR_5g098170 | 43.824 | 340 | 181 | 6 | 10 | 347 | 32 | 363 | 4.05e-90 | 275 |
| MsG0780036378.01.T01 | MTR_7g118300 | 43.642 | 346 | 185 | 7 | 3 | 347 | 39 | 375 | 1.30e-87 | 268 |
| MsG0780036378.01.T01 | MTR_0591s0010 | 41.880 | 351 | 187 | 9 | 3 | 347 | 38 | 377 | 2.31e-82 | 255 |
| MsG0780036378.01.T01 | MTR_1g050220 | 45.015 | 331 | 129 | 6 | 1 | 330 | 9 | 287 | 5.66e-82 | 251 |
| MsG0780036378.01.T01 | MTR_8g068690 | 41.547 | 349 | 191 | 8 | 3 | 347 | 37 | 376 | 1.36e-81 | 253 |
| MsG0780036378.01.T01 | MTR_1g036510 | 53.386 | 251 | 112 | 4 | 1 | 250 | 40 | 286 | 4.57e-80 | 246 |
| MsG0780036378.01.T01 | MTR_8g068750 | 40.401 | 349 | 195 | 8 | 3 | 347 | 38 | 377 | 3.73e-79 | 247 |
| MsG0780036378.01.T01 | MTR_8g068870 | 40.870 | 345 | 191 | 8 | 7 | 347 | 28 | 363 | 9.54e-79 | 245 |
| MsG0780036378.01.T01 | MTR_7g118320 | 40.517 | 348 | 196 | 7 | 3 | 347 | 23 | 362 | 1.73e-78 | 244 |
| MsG0780036378.01.T01 | MTR_8g024160 | 40.173 | 346 | 157 | 7 | 3 | 347 | 32 | 328 | 3.68e-75 | 235 |
| MsG0780036378.01.T01 | MTR_1g036510 | 51.092 | 229 | 107 | 4 | 1 | 228 | 40 | 264 | 5.78e-67 | 212 |
| MsG0780036378.01.T01 | MTR_6g078850 | 37.892 | 351 | 205 | 7 | 1 | 346 | 40 | 382 | 4.79e-65 | 214 |
| MsG0780036378.01.T01 | MTR_8g068570 | 39.432 | 317 | 153 | 7 | 3 | 315 | 38 | 319 | 8.85e-65 | 208 |
| MsG0780036378.01.T01 | MTR_8g068730 | 35.817 | 349 | 179 | 9 | 3 | 347 | 38 | 345 | 1.00e-60 | 198 |
| MsG0780036378.01.T01 | MTR_2g072790 | 34.337 | 332 | 201 | 8 | 5 | 331 | 20 | 339 | 1.63e-57 | 190 |
| MsG0780036378.01.T01 | MTR_3g096050 | 37.627 | 295 | 169 | 6 | 59 | 347 | 73 | 358 | 1.36e-56 | 188 |
| MsG0780036378.01.T01 | MTR_8g068690 | 41.600 | 250 | 135 | 6 | 3 | 249 | 37 | 278 | 5.34e-55 | 182 |
| MsG0780036378.01.T01 | MTR_3g096070 | 38.028 | 284 | 141 | 6 | 59 | 339 | 73 | 324 | 1.60e-54 | 182 |
| MsG0780036378.01.T01 | MTR_3g083620 | 33.742 | 326 | 196 | 8 | 11 | 332 | 38 | 347 | 7.17e-53 | 178 |
| MsG0780036378.01.T01 | MTR_0079s0070 | 35.258 | 329 | 190 | 10 | 12 | 333 | 9 | 321 | 3.71e-51 | 173 |
| MsG0780036378.01.T01 | MTR_4g038440 | 35.258 | 329 | 190 | 10 | 12 | 333 | 43 | 355 | 1.30e-50 | 172 |
| MsG0780036378.01.T01 | MTR_1g076940 | 29.846 | 325 | 215 | 5 | 12 | 332 | 32 | 347 | 1.32e-49 | 170 |
| MsG0780036378.01.T01 | MTR_0017s0100 | 32.059 | 340 | 209 | 10 | 13 | 346 | 44 | 367 | 3.21e-49 | 169 |
| MsG0780036378.01.T01 | MTR_8g068600 | 38.314 | 261 | 142 | 7 | 18 | 267 | 27 | 279 | 1.06e-46 | 160 |
| MsG0780036378.01.T01 | MTR_6g060290 | 54.037 | 161 | 53 | 4 | 1 | 161 | 31 | 170 | 1.07e-46 | 156 |
| MsG0780036378.01.T01 | MTR_4g088340 | 29.825 | 342 | 219 | 8 | 10 | 346 | 7 | 332 | 9.86e-46 | 159 |
| MsG0780036378.01.T01 | MTR_4g088345 | 30.117 | 342 | 218 | 8 | 10 | 346 | 30 | 355 | 1.51e-45 | 159 |
| MsG0780036378.01.T01 | MTR_5g074600 | 31.317 | 281 | 178 | 7 | 61 | 333 | 73 | 346 | 9.10e-45 | 157 |
| MsG0780036378.01.T01 | MTR_4g033015 | 32.653 | 343 | 206 | 9 | 12 | 342 | 32 | 361 | 2.57e-44 | 156 |
| MsG0780036378.01.T01 | MTR_7g024520 | 28.986 | 345 | 208 | 10 | 10 | 345 | 29 | 345 | 1.49e-43 | 154 |
| MsG0780036378.01.T01 | MTR_8g068740 | 36.122 | 263 | 125 | 7 | 3 | 262 | 38 | 260 | 1.74e-43 | 151 |
| MsG0780036378.01.T01 | MTR_4g033200 | 32.362 | 343 | 207 | 9 | 12 | 342 | 32 | 361 | 2.25e-43 | 154 |
| MsG0780036378.01.T01 | MTR_7g014570 | 29.268 | 328 | 202 | 9 | 13 | 332 | 33 | 338 | 2.39e-43 | 153 |
| MsG0780036378.01.T01 | MTR_5g074680 | 27.887 | 355 | 220 | 9 | 6 | 346 | 27 | 359 | 4.86e-42 | 150 |
| MsG0780036378.01.T01 | MTR_7g024500 | 28.024 | 339 | 219 | 8 | 10 | 345 | 29 | 345 | 5.22e-42 | 149 |
| MsG0780036378.01.T01 | MTR_7g014510 | 27.566 | 341 | 223 | 9 | 13 | 346 | 33 | 356 | 7.59e-42 | 149 |
| MsG0780036378.01.T01 | MTR_4g033150 | 31.195 | 343 | 211 | 9 | 12 | 342 | 32 | 361 | 9.39e-42 | 149 |
| MsG0780036378.01.T01 | MTR_5g074580 | 31.950 | 241 | 155 | 4 | 110 | 345 | 119 | 355 | 6.42e-41 | 147 |
| MsG0780036378.01.T01 | MTR_5g074540 | 32.432 | 222 | 145 | 3 | 117 | 333 | 107 | 328 | 4.70e-39 | 142 |
| MsG0780036378.01.T01 | MTR_4g079160 | 29.395 | 347 | 215 | 10 | 1 | 333 | 12 | 342 | 1.03e-38 | 141 |
| MsG0780036378.01.T01 | MTR_4g088190 | 30.952 | 336 | 214 | 7 | 6 | 333 | 26 | 351 | 3.13e-38 | 140 |
| MsG0780036378.01.T01 | MTR_4g088200 | 29.552 | 335 | 217 | 8 | 6 | 333 | 26 | 348 | 1.02e-37 | 139 |
| MsG0780036378.01.T01 | MTR_3g096050 | 39.896 | 193 | 110 | 3 | 59 | 250 | 73 | 260 | 2.75e-37 | 135 |
| MsG0780036378.01.T01 | MTR_5g074610 | 29.600 | 250 | 141 | 6 | 103 | 346 | 105 | 325 | 3.73e-34 | 128 |
| MsG0780036378.01.T01 | MTR_8g068620 | 53.211 | 109 | 51 | 0 | 141 | 249 | 2 | 110 | 6.10e-34 | 121 |
| MsG0780036378.01.T01 | MTR_1g076930 | 29.600 | 250 | 153 | 7 | 10 | 252 | 7 | 240 | 1.48e-31 | 119 |
| MsG0780036378.01.T01 | MTR_4g033170 | 30.075 | 266 | 162 | 8 | 12 | 271 | 32 | 279 | 3.15e-29 | 115 |
| MsG0780036378.01.T01 | MTR_4g033085 | 29.811 | 265 | 162 | 8 | 12 | 270 | 32 | 278 | 7.04e-28 | 112 |
| MsG0780036378.01.T01 | MTR_4g038440 | 33.610 | 241 | 144 | 6 | 13 | 250 | 44 | 271 | 1.27e-27 | 109 |
| MsG0780036378.01.T01 | MTR_0794s0020 | 29.437 | 231 | 143 | 7 | 12 | 235 | 32 | 249 | 3.31e-23 | 97.1 |
| MsG0780036378.01.T01 | MTR_6g060280 | 70.909 | 55 | 11 | 1 | 201 | 250 | 1 | 55 | 7.32e-19 | 80.1 |
| MsG0780036378.01.T01 | MTR_8g068760 | 39.000 | 100 | 59 | 2 | 249 | 347 | 135 | 233 | 1.61e-15 | 75.5 |
| MsG0780036378.01.T01 | MTR_8g068580 | 41.304 | 92 | 52 | 2 | 257 | 347 | 43 | 133 | 2.05e-12 | 64.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036378.01.T01 | AT5G54160 | 46.418 | 349 | 174 | 7 | 1 | 347 | 24 | 361 | 1.16e-96 | 291 |
| MsG0780036378.01.T01 | AT1G77520 | 40.625 | 352 | 196 | 6 | 3 | 347 | 36 | 381 | 1.55e-88 | 271 |
| MsG0780036378.01.T01 | AT1G51990 | 38.889 | 342 | 195 | 6 | 10 | 346 | 29 | 361 | 6.90e-78 | 243 |
| MsG0780036378.01.T01 | AT1G77530 | 41.477 | 352 | 193 | 7 | 3 | 347 | 36 | 381 | 1.23e-77 | 243 |
| MsG0780036378.01.T01 | AT1G51990 | 38.304 | 342 | 197 | 6 | 10 | 346 | 29 | 361 | 3.56e-77 | 241 |
| MsG0780036378.01.T01 | AT1G63140 | 40.625 | 352 | 196 | 8 | 3 | 347 | 36 | 381 | 5.05e-77 | 241 |
| MsG0780036378.01.T01 | AT1G63140 | 40.113 | 354 | 195 | 8 | 3 | 347 | 56 | 401 | 5.67e-77 | 242 |
| MsG0780036378.01.T01 | AT1G21100 | 39.412 | 340 | 194 | 8 | 3 | 337 | 32 | 364 | 9.20e-72 | 228 |
| MsG0780036378.01.T01 | AT1G21130 | 39.706 | 340 | 193 | 9 | 3 | 337 | 32 | 364 | 1.51e-71 | 227 |
| MsG0780036378.01.T01 | AT1G21110 | 39.706 | 340 | 193 | 9 | 3 | 337 | 32 | 364 | 5.55e-71 | 226 |
| MsG0780036378.01.T01 | AT1G33030 | 38.551 | 345 | 198 | 7 | 1 | 340 | 11 | 346 | 8.59e-71 | 224 |
| MsG0780036378.01.T01 | AT5G53810 | 38.462 | 351 | 192 | 9 | 10 | 347 | 39 | 378 | 5.08e-70 | 223 |
| MsG0780036378.01.T01 | AT1G21120 | 39.412 | 340 | 194 | 9 | 3 | 337 | 32 | 364 | 1.09e-69 | 222 |
| MsG0780036378.01.T01 | AT1G21120 | 39.118 | 340 | 195 | 9 | 3 | 337 | 80 | 412 | 7.34e-69 | 222 |
| MsG0780036378.01.T01 | AT1G76790 | 39.093 | 353 | 198 | 10 | 3 | 347 | 22 | 365 | 3.45e-68 | 218 |
| MsG0780036378.01.T01 | AT3G53140 | 34.118 | 340 | 203 | 7 | 1 | 331 | 17 | 344 | 1.76e-61 | 201 |
| MsG0780036378.01.T01 | AT1G62900 | 44.608 | 204 | 110 | 2 | 146 | 347 | 3 | 205 | 2.02e-57 | 185 |
| MsG0780036378.01.T01 | AT5G37170 | 36.677 | 319 | 161 | 8 | 37 | 347 | 49 | 334 | 1.83e-53 | 179 |
| MsG0780036378.01.T01 | AT5G37170 | 43.939 | 198 | 107 | 3 | 153 | 347 | 38 | 234 | 6.90e-52 | 172 |
| MsG0780036378.01.T01 | AT5G37170 | 43.850 | 187 | 101 | 3 | 164 | 347 | 1 | 186 | 3.58e-48 | 160 |
| MsG0780036378.01.T01 | AT1G63140 | 40.711 | 253 | 140 | 6 | 3 | 250 | 56 | 303 | 3.02e-47 | 162 |
| MsG0780036378.01.T01 | AT1G33030 | 37.008 | 254 | 149 | 5 | 1 | 252 | 11 | 255 | 2.57e-45 | 156 |
| MsG0780036378.01.T01 | AT1G33030 | 36.364 | 264 | 155 | 6 | 1 | 260 | 11 | 265 | 3.87e-45 | 155 |
| MsG0780036378.01.T01 | AT1G21130 | 40.239 | 251 | 140 | 8 | 3 | 250 | 32 | 275 | 5.15e-42 | 148 |
| MsG0780036378.01.T01 | AT1G77530 | 41.631 | 233 | 126 | 5 | 3 | 230 | 36 | 263 | 1.20e-41 | 146 |
| MsG0780036378.01.T01 | AT4G35160 | 30.000 | 340 | 204 | 11 | 11 | 332 | 44 | 367 | 5.51e-38 | 140 |
| MsG0780036378.01.T01 | AT4G35150 | 34.483 | 203 | 123 | 3 | 140 | 332 | 108 | 310 | 1.32e-37 | 137 |
| MsG0780036378.01.T01 | AT1G63140 | 38.655 | 238 | 136 | 6 | 3 | 235 | 56 | 288 | 1.24e-36 | 134 |
Find 71 sgRNAs with CRISPR-Local
Find 102 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCTAAAGAATTTGAGAGTT+TGG | 0.219073 | 7:-7302579 | MsG0780036378.01.T01:CDS |
| CGACAAACAACTTGGAATTT+AGG | 0.243482 | 7:+7302539 | None:intergenic |
| TTTGTTGACTCTCGATCTTC+TGG | 0.250742 | 7:+7302668 | None:intergenic |
| TTGAATCACCCAGCTTTCTT+AGG | 0.281972 | 7:-7303964 | MsG0780036378.01.T01:CDS |
| AGCATACGGTCAAGCCTATT+TGG | 0.295943 | 7:+7304138 | None:intergenic |
| AAGTCGAGGTATTGAGTTAT+TGG | 0.316061 | 7:+7304162 | None:intergenic |
| TCTAAAGAATTTGAGAGTTT+GGG | 0.318424 | 7:-7302578 | MsG0780036378.01.T01:CDS |
| CATATCCTTTGTATATTTCA+AGG | 0.318611 | 7:+7303372 | None:intergenic |
| AGCATTATAGCATCTCCTTC+TGG | 0.323700 | 7:+7303073 | None:intergenic |
| TGTAGCTTGTGTTCCATAAT+TGG | 0.338384 | 7:-7302778 | MsG0780036378.01.T01:intron |
| CGGAAACAAGGATTATTATA+TGG | 0.352656 | 7:-7304004 | MsG0780036378.01.T01:CDS |
| GAGAGTTTGGGTAAACAATC+TGG | 0.372091 | 7:-7302566 | MsG0780036378.01.T01:CDS |
| AACGTATTCGAGAAAGTTCA+TGG | 0.380926 | 7:-7303491 | MsG0780036378.01.T01:CDS |
| TTCTCGAATACGTTTATCTC+TGG | 0.389709 | 7:+7303500 | None:intergenic |
| AATACCTCGACTTACCAAAT+AGG | 0.391861 | 7:-7304152 | MsG0780036378.01.T01:CDS |
| CACAAAGCTTTGCCTTCAAA+TGG | 0.393394 | 7:-7302719 | MsG0780036378.01.T01:CDS |
| ATAATGACTTTACCATTTGA+AGG | 0.398844 | 7:+7302707 | None:intergenic |
| TGAAATATACAAAGGATATG+AGG | 0.400826 | 7:-7303369 | MsG0780036378.01.T01:CDS |
| TTCTCAAAGTATTCATAGAT+AGG | 0.406331 | 7:+7303464 | None:intergenic |
| AACTGCAGGAAACACCATAT+TGG | 0.407144 | 7:+7304290 | None:intergenic |
| CATGATTATAGCTTCCAATA+TGG | 0.408040 | 7:-7304304 | MsG0780036378.01.T01:CDS |
| TGTCGTGCTTTCTCCATCCT+TGG | 0.409600 | 7:-7302521 | MsG0780036378.01.T01:CDS |
| CTGCGCTTGCTTGCTAGCCA+CGG | 0.445823 | 7:-7304117 | MsG0780036378.01.T01:CDS |
| TAGCAAGCAAGCGCAGCATA+CGG | 0.457537 | 7:+7304124 | None:intergenic |
| AGGAGATGCTATAATGCTTA+AGG | 0.460184 | 7:-7303068 | MsG0780036378.01.T01:intron |
| GTGATATAGGTCGAATTTGA+AGG | 0.465211 | 7:-7303537 | MsG0780036378.01.T01:intron |
| TTTCTCCATCCTTGGTGTGA+TGG | 0.466015 | 7:-7302513 | MsG0780036378.01.T01:CDS |
| CTTGTTTGATATCATTGCTA+AGG | 0.474167 | 7:-7304244 | MsG0780036378.01.T01:CDS |
| CACCATGCTACCATCATCAT+TGG | 0.480009 | 7:+7304071 | None:intergenic |
| ATCATTGGTTCGAGTGCAAA+CGG | 0.482861 | 7:+7304086 | None:intergenic |
| TACCACACTCCTAAGAAAGC+TGG | 0.484096 | 7:+7303955 | None:intergenic |
| GTTGATGTAGGAGGTGGAAA+TGG | 0.497071 | 7:-7303332 | MsG0780036378.01.T01:CDS |
| ATATCACATGAAAACAATGT+TGG | 0.508642 | 7:-7304219 | MsG0780036378.01.T01:CDS |
| AGAAAGCACGACAAACAACT+TGG | 0.515962 | 7:+7302531 | None:intergenic |
| GTTTATCACAGCTGGTGGTA+AGG | 0.522095 | 7:-7302609 | MsG0780036378.01.T01:CDS |
| AAATGGTAAAGTCATTATTG+TGG | 0.527665 | 7:-7302702 | MsG0780036378.01.T01:CDS |
| AATAATCCTTGTTTCCGTGA+TGG | 0.532543 | 7:+7304010 | None:intergenic |
| AACATGATGTTTATCACAGC+TGG | 0.539235 | 7:-7302617 | MsG0780036378.01.T01:CDS |
| AAGTTCCATCACACCAAGGA+TGG | 0.539267 | 7:+7302508 | None:intergenic |
| CTTTGTCCATCACGGAAACA+AGG | 0.541409 | 7:-7304016 | MsG0780036378.01.T01:CDS |
| GGTGCATGTTCAATCACTTG+TGG | 0.542683 | 7:+7303248 | None:intergenic |
| CATATTGGAAGCTATAATCA+TGG | 0.546612 | 7:+7304305 | None:intergenic |
| GTTTATGCAATCACACCTCC+TGG | 0.548500 | 7:-7304045 | MsG0780036378.01.T01:CDS |
| GTGCATGTTCAATCACTTGT+GGG | 0.549965 | 7:+7303249 | None:intergenic |
| GAAATATACAAAGGATATGA+GGG | 0.554252 | 7:-7303368 | MsG0780036378.01.T01:CDS |
| ACCACACTCCTAAGAAAGCT+GGG | 0.554693 | 7:+7303956 | None:intergenic |
| TGCACTCGAACCAATGATGA+TGG | 0.562264 | 7:-7304081 | MsG0780036378.01.T01:CDS |
| TTAGGGATCGAACATGTTGG+AGG | 0.564879 | 7:-7303115 | MsG0780036378.01.T01:intron |
| TGGTTCGAGTGCAAACGGAC+AGG | 0.566921 | 7:+7304091 | None:intergenic |
| GAACATGCACCACCCTCCCC+AGG | 0.567140 | 7:-7303236 | MsG0780036378.01.T01:intron |
| GGTAAATACTTTGTCCATCA+CGG | 0.568548 | 7:-7304024 | MsG0780036378.01.T01:CDS |
| TATTTAGGGATCGAACATGT+TGG | 0.571558 | 7:-7303118 | MsG0780036378.01.T01:intron |
| ATATCAACACTGGTTGATGT+AGG | 0.573750 | 7:-7303344 | MsG0780036378.01.T01:CDS |
| ACACTGGTTGATGTAGGAGG+TGG | 0.577123 | 7:-7303338 | MsG0780036378.01.T01:CDS |
| GATCAAACAATAACCTGGGG+AGG | 0.599662 | 7:+7303223 | None:intergenic |
| ACCCAGCTTTCTTAGGAGTG+TGG | 0.599760 | 7:-7303957 | MsG0780036378.01.T01:intron |
| AAACAATAACCTGGGGAGGG+TGG | 0.603753 | 7:+7303227 | None:intergenic |
| TGATGGACAAAGTATTTACC+AGG | 0.605667 | 7:+7304027 | None:intergenic |
| ATGTTTGAATGTGTTCCAGA+AGG | 0.610554 | 7:-7303088 | MsG0780036378.01.T01:CDS |
| AAACAATGTTGGATTTGTGT+CGG | 0.622769 | 7:-7304208 | MsG0780036378.01.T01:CDS |
| ATAATCCTTGAAATATACAA+AGG | 0.628176 | 7:-7303377 | MsG0780036378.01.T01:CDS |
| ATCAAACAATAACCTGGGGA+GGG | 0.629339 | 7:+7303224 | None:intergenic |
| TCAACACTGGTTGATGTAGG+AGG | 0.630452 | 7:-7303341 | MsG0780036378.01.T01:CDS |
| GCTTGTGTTCCATAATTGGT+CGG | 0.631020 | 7:-7302774 | MsG0780036378.01.T01:CDS |
| CAAGCCTATTTGGTAAGTCG+AGG | 0.635008 | 7:+7304148 | None:intergenic |
| ATGATGTTTATCACAGCTGG+TGG | 0.648385 | 7:-7302614 | MsG0780036378.01.T01:CDS |
| AACCAATGATGATGGTAGCA+TGG | 0.656306 | 7:-7304073 | MsG0780036378.01.T01:CDS |
| ATATGAGGGAATATCAACAC+TGG | 0.673093 | 7:-7303354 | MsG0780036378.01.T01:CDS |
| TGCAAACGGACAGGAGACCG+TGG | 0.674214 | 7:+7304100 | None:intergenic |
| TGTAAAGTTCCATCACACCA+AGG | 0.691403 | 7:+7302504 | None:intergenic |
| TGGACAAAGTATTTACCAGG+AGG | 0.738714 | 7:+7304030 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAAATAACTATATTAAGTTA+TGG | + | Chr7:7302882-7302901 | None:intergenic | 10.0% |
| !!! | AATATTGTCAAATTTTATTT+AGG | - | Chr7:7303669-7303688 | MsG0780036378.01.T01:intron | 10.0% |
| !!! | ATATTGTCAAATTTTATTTA+GGG | - | Chr7:7303670-7303689 | MsG0780036378.01.T01:intron | 10.0% |
| !! | AATTTCAAATAATCTTATAG+TGG | + | Chr7:7303177-7303196 | None:intergenic | 15.0% |
| !! | ATGAATTCAAATGATAATAT+AGG | - | Chr7:7303084-7303103 | MsG0780036378.01.T01:CDS | 15.0% |
| !! | TTAATGGCATTTATATATAA+AGG | + | Chr7:7303909-7303928 | None:intergenic | 15.0% |
| !!! | AAAAATCATTTTTGTGATAT+AGG | - | Chr7:7303252-7303271 | MsG0780036378.01.T01:CDS | 15.0% |
| !! | AATAAGATTAAATAGTATGC+TGG | - | Chr7:7302906-7302925 | MsG0780036378.01.T01:intron | 20.0% |
| !! | ACATTCAAAACAAATAATCT+TGG | + | Chr7:7303636-7303655 | None:intergenic | 20.0% |
| !! | ATAATCCTTGAAATATACAA+AGG | - | Chr7:7303425-7303444 | MsG0780036378.01.T01:CDS | 20.0% |
| !!! | AAATTGATTGCTTTTATTGA+TGG | + | Chr7:7303527-7303546 | None:intergenic | 20.0% |
| ! | AAATGGTAAAGTCATTATTG+TGG | - | Chr7:7304100-7304119 | MsG0780036378.01.T01:CDS | 25.0% |
| ! | AACAAATAATCTTGGAAAAG+TGG | + | Chr7:7303628-7303647 | None:intergenic | 25.0% |
| ! | ATAATGACTTTACCATTTGA+AGG | + | Chr7:7304098-7304117 | None:intergenic | 25.0% |
| ! | ATATCACATGAAAACAATGT+TGG | - | Chr7:7302583-7302602 | MsG0780036378.01.T01:CDS | 25.0% |
| ! | CATATCCTTTGTATATTTCA+AGG | + | Chr7:7303433-7303452 | None:intergenic | 25.0% |
| ! | CTTCAATTTCACATCATATA+TGG | + | Chr7:7303054-7303073 | None:intergenic | 25.0% |
| ! | GAAATATACAAAGGATATGA+GGG | - | Chr7:7303434-7303453 | MsG0780036378.01.T01:CDS | 25.0% |
| ! | TCTAAAGAATTTGAGAGTTT+GGG | - | Chr7:7304224-7304243 | MsG0780036378.01.T01:CDS | 25.0% |
| ! | TGAAATATACAAAGGATATG+AGG | - | Chr7:7303433-7303452 | MsG0780036378.01.T01:CDS | 25.0% |
| ! | TGGCATTTATATATAAAGGT+TGG | + | Chr7:7303905-7303924 | None:intergenic | 25.0% |
| !! | AAATGATCATCTTCTTTTGA+TGG | - | Chr7:7303785-7303804 | MsG0780036378.01.T01:intron | 25.0% |
| !! | CGAGTACAATTTTATAAGTT+TGG | + | Chr7:7303110-7303129 | None:intergenic | 25.0% |
| !! | TTCTAAAGAATTTGAGAGTT+TGG | - | Chr7:7304223-7304242 | MsG0780036378.01.T01:CDS | 25.0% |
| !! | TTCTCAAAGTATTCATAGAT+AGG | + | Chr7:7303341-7303360 | None:intergenic | 25.0% |
| !!! | GCTATTTGATCGTTTATTTT+CGG | + | Chr7:7302991-7303010 | None:intergenic | 25.0% |
| !!! | TGCTTTTATTGATGGATATT+TGG | + | Chr7:7303519-7303538 | None:intergenic | 25.0% |
| AAACAATGTTGGATTTGTGT+CGG | - | Chr7:7302594-7302613 | MsG0780036378.01.T01:CDS | 30.0% | |
| AGATGATCAAACAATAACCT+GGG | + | Chr7:7303586-7303605 | None:intergenic | 30.0% | |
| AGTGTCCAAGTTAAACATTT+AGG | + | Chr7:7303014-7303033 | None:intergenic | 30.0% | |
| ATAGCCCTAAATGTTTAACT+TGG | - | Chr7:7303006-7303025 | MsG0780036378.01.T01:intron | 30.0% | |
| CGGAAACAAGGATTATTATA+TGG | - | Chr7:7302798-7302817 | MsG0780036378.01.T01:intron | 30.0% | |
| CTTGTTTGATATCATTGCTA+AGG | - | Chr7:7302558-7302577 | MsG0780036378.01.T01:CDS | 30.0% | |
| GTGTCCAAGTTAAACATTTA+GGG | + | Chr7:7303013-7303032 | None:intergenic | 30.0% | |
| TAATCACGTGTAGATATAAG+TGG | + | Chr7:7302949-7302968 | None:intergenic | 30.0% | |
| ! | ATTTTTCATCCGACCAATTA+TGG | + | Chr7:7304040-7304059 | None:intergenic | 30.0% |
| AACATGATGTTTATCACAGC+TGG | - | Chr7:7304185-7304204 | MsG0780036378.01.T01:CDS | 35.0% | |
| AACGTATTCGAGAAAGTTCA+TGG | - | Chr7:7303311-7303330 | MsG0780036378.01.T01:CDS | 35.0% | |
| AATACCTCGACTTACCAAAT+AGG | - | Chr7:7302650-7302669 | MsG0780036378.01.T01:CDS | 35.0% | |
| AGGAGATGCTATAATGCTTA+AGG | - | Chr7:7303734-7303753 | MsG0780036378.01.T01:intron | 35.0% | |
| ATATGAGGGAATATCAACAC+TGG | - | Chr7:7303448-7303467 | MsG0780036378.01.T01:CDS | 35.0% | |
| ATGTTTGAATGTGTTCCAGA+AGG | - | Chr7:7303714-7303733 | MsG0780036378.01.T01:intron | 35.0% | |
| CGACAAACAACTTGGAATTT+AGG | + | Chr7:7304266-7304285 | None:intergenic | 35.0% | |
| GAGATGATCAAACAATAACC+TGG | + | Chr7:7303587-7303606 | None:intergenic | 35.0% | |
| GATGATCAAACAATAACCTG+GGG | + | Chr7:7303585-7303604 | None:intergenic | 35.0% | |
| GATGCTATAATGCTTAAGGT+TGG | - | Chr7:7303738-7303757 | MsG0780036378.01.T01:intron | 35.0% | |
| GGTAAATACTTTGTCCATCA+CGG | - | Chr7:7302778-7302797 | MsG0780036378.01.T01:intron | 35.0% | |
| GTGATATAGGTCGAATTTGA+AGG | - | Chr7:7303265-7303284 | MsG0780036378.01.T01:CDS | 35.0% | |
| TAGACCTCAAGTTAGCAAAA+TGG | + | Chr7:7303875-7303894 | None:intergenic | 35.0% | |
| TATTTAGGGATCGAACATGT+TGG | - | Chr7:7303684-7303703 | MsG0780036378.01.T01:intron | 35.0% | |
| TTCTCGAATACGTTTATCTC+TGG | + | Chr7:7303305-7303324 | None:intergenic | 35.0% | |
| ! | AATAATCCTTGTTTCCGTGA+TGG | + | Chr7:7302795-7302814 | None:intergenic | 35.0% |
| ! | ATATCAACACTGGTTGATGT+AGG | - | Chr7:7303458-7303477 | MsG0780036378.01.T01:CDS | 35.0% |
| ! | TGATGGACAAAGTATTTACC+AGG | + | Chr7:7302778-7302797 | None:intergenic | 35.0% |
| ! | TGTAGCTTGTGTTCCATAAT+TGG | - | Chr7:7304024-7304043 | MsG0780036378.01.T01:CDS | 35.0% |
| !! | AAGTCGAGGTATTGAGTTAT+TGG | + | Chr7:7302643-7302662 | None:intergenic | 35.0% |
| !! | ATTGCAGCTTTAAAAACTGC+AGG | + | Chr7:7302529-7302548 | None:intergenic | 35.0% |
| AACTGCAGGAAACACCATAT+TGG | + | Chr7:7302515-7302534 | None:intergenic | 40.0% | |
| ACCTCAAGTTAGCAAAATGG+CGG | + | Chr7:7303872-7303891 | None:intergenic | 40.0% | |
| AGCATTATAGCATCTCCTTC+TGG | + | Chr7:7303732-7303751 | None:intergenic | 40.0% | |
| ATCAAACAATAACCTGGGGA+GGG | + | Chr7:7303581-7303600 | None:intergenic | 40.0% | |
| ATGATGTTTATCACAGCTGG+TGG | - | Chr7:7304188-7304207 | MsG0780036378.01.T01:CDS | 40.0% | |
| CACAAAGCTTTGCCTTCAAA+TGG | - | Chr7:7304083-7304102 | MsG0780036378.01.T01:CDS | 40.0% | |
| GAGAGTTTGGGTAAACAATC+TGG | - | Chr7:7304236-7304255 | MsG0780036378.01.T01:CDS | 40.0% | |
| GCTTGTGTTCCATAATTGGT+CGG | - | Chr7:7304028-7304047 | MsG0780036378.01.T01:CDS | 40.0% | |
| GTGCATGTTCAATCACTTGT+GGG | + | Chr7:7303556-7303575 | None:intergenic | 40.0% | |
| TGTAAAGTTCCATCACACCA+AGG | + | Chr7:7304301-7304320 | None:intergenic | 40.0% | |
| TTGAATCACCCAGCTTTCTT+AGG | - | Chr7:7302838-7302857 | MsG0780036378.01.T01:intron | 40.0% | |
| ! | AGAAAGCACGACAAACAACT+TGG | + | Chr7:7304274-7304293 | None:intergenic | 40.0% |
| ! | CGGAGGATTGTTATGTTTTC+AGG | + | Chr7:7303852-7303871 | None:intergenic | 40.0% |
| ! | TGGACAAAGTATTTACCAGG+AGG | + | Chr7:7302775-7302794 | None:intergenic | 40.0% |
| !! | AACCAATGATGATGGTAGCA+TGG | - | Chr7:7302729-7302748 | MsG0780036378.01.T01:CDS | 40.0% |
| !! | ATCATTGGTTCGAGTGCAAA+CGG | + | Chr7:7302719-7302738 | None:intergenic | 40.0% |
| !! | TTTGTTGACTCTCGATCTTC+TGG | + | Chr7:7304137-7304156 | None:intergenic | 40.0% |
| AAGTTCCATCACACCAAGGA+TGG | + | Chr7:7304297-7304316 | None:intergenic | 45.0% | |
| ACCACACTCCTAAGAAAGCT+GGG | + | Chr7:7302849-7302868 | None:intergenic | 45.0% | |
| AGCATACGGTCAAGCCTATT+TGG | + | Chr7:7302667-7302686 | None:intergenic | 45.0% | |
| CAAGCCTATTTGGTAAGTCG+AGG | + | Chr7:7302657-7302676 | None:intergenic | 45.0% | |
| CACCATGCTACCATCATCAT+TGG | + | Chr7:7302734-7302753 | None:intergenic | 45.0% | |
| CTTTGTCCATCACGGAAACA+AGG | - | Chr7:7302786-7302805 | MsG0780036378.01.T01:intron | 45.0% | |
| GATCAAACAATAACCTGGGG+AGG | + | Chr7:7303582-7303601 | None:intergenic | 45.0% | |
| GGTGCATGTTCAATCACTTG+TGG | + | Chr7:7303557-7303576 | None:intergenic | 45.0% | |
| GTTGATGTAGGAGGTGGAAA+TGG | - | Chr7:7303470-7303489 | MsG0780036378.01.T01:CDS | 45.0% | |
| GTTTATCACAGCTGGTGGTA+AGG | - | Chr7:7304193-7304212 | MsG0780036378.01.T01:CDS | 45.0% | |
| GTTTATGCAATCACACCTCC+TGG | - | Chr7:7302757-7302776 | MsG0780036378.01.T01:CDS | 45.0% | |
| TACCACACTCCTAAGAAAGC+TGG | + | Chr7:7302850-7302869 | None:intergenic | 45.0% | |
| TCAAGTTAGCAAAATGGCGG+AGG | + | Chr7:7303869-7303888 | None:intergenic | 45.0% | |
| TGCACTCGAACCAATGATGA+TGG | - | Chr7:7302721-7302740 | MsG0780036378.01.T01:CDS | 45.0% | |
| TTAGGGATCGAACATGTTGG+AGG | - | Chr7:7303687-7303706 | MsG0780036378.01.T01:intron | 45.0% | |
| ! | TCAACACTGGTTGATGTAGG+AGG | - | Chr7:7303461-7303480 | MsG0780036378.01.T01:CDS | 45.0% |
| ! | TTTCTCCATCCTTGGTGTGA+TGG | - | Chr7:7304289-7304308 | MsG0780036378.01.T01:CDS | 45.0% |
| !! | TCCGCCATTTTGCTAACTTG+AGG | - | Chr7:7303868-7303887 | MsG0780036378.01.T01:intron | 45.0% |
| !!! | ATATTTATGAATTTTTTTAA+TGG | + | Chr7:7303925-7303944 | None:intergenic | 5.0% |
| AAACAATAACCTGGGGAGGG+TGG | + | Chr7:7303578-7303597 | None:intergenic | 50.0% | |
| ACCCAGCTTTCTTAGGAGTG+TGG | - | Chr7:7302845-7302864 | MsG0780036378.01.T01:intron | 50.0% | |
| TAGCAAGCAAGCGCAGCATA+CGG | + | Chr7:7302681-7302700 | None:intergenic | 50.0% | |
| TGTCGTGCTTTCTCCATCCT+TGG | - | Chr7:7304281-7304300 | MsG0780036378.01.T01:CDS | 50.0% | |
| ! | ACACTGGTTGATGTAGGAGG+TGG | - | Chr7:7303464-7303483 | MsG0780036378.01.T01:CDS | 50.0% |
| TGGTTCGAGTGCAAACGGAC+AGG | + | Chr7:7302714-7302733 | None:intergenic | 55.0% | |
| TGCAAACGGACAGGAGACCG+TGG | + | Chr7:7302705-7302724 | None:intergenic | 60.0% | |
| ! | CTGCGCTTGCTTGCTAGCCA+CGG | - | Chr7:7302685-7302704 | MsG0780036378.01.T01:CDS | 60.0% |
| GAACATGCACCACCCTCCCC+AGG | - | Chr7:7303566-7303585 | MsG0780036378.01.T01:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 7302499 | 7304325 | 7302499 | ID=MsG0780036378.01;Name=MsG0780036378.01 |
| Chr7 | mRNA | 7302499 | 7304325 | 7302499 | ID=MsG0780036378.01.T01;Parent=MsG0780036378.01;Name=MsG0780036378.01.T01;_AED=0.25;_eAED=0.25;_QI=0|0|0|1|1|1|4|0|347 |
| Chr7 | exon | 7303958 | 7304325 | 7303958 | ID=MsG0780036378.01.T01:exon:3888;Parent=MsG0780036378.01.T01 |
| Chr7 | exon | 7303237 | 7303550 | 7303237 | ID=MsG0780036378.01.T01:exon:3887;Parent=MsG0780036378.01.T01 |
| Chr7 | exon | 7303069 | 7303133 | 7303069 | ID=MsG0780036378.01.T01:exon:3886;Parent=MsG0780036378.01.T01 |
| Chr7 | exon | 7302499 | 7302795 | 7302499 | ID=MsG0780036378.01.T01:exon:3885;Parent=MsG0780036378.01.T01 |
| Chr7 | CDS | 7303958 | 7304325 | 7303958 | ID=MsG0780036378.01.T01:cds;Parent=MsG0780036378.01.T01 |
| Chr7 | CDS | 7303237 | 7303550 | 7303237 | ID=MsG0780036378.01.T01:cds;Parent=MsG0780036378.01.T01 |
| Chr7 | CDS | 7303069 | 7303133 | 7303069 | ID=MsG0780036378.01.T01:cds;Parent=MsG0780036378.01.T01 |
| Chr7 | CDS | 7302499 | 7302795 | 7302499 | ID=MsG0780036378.01.T01:cds;Parent=MsG0780036378.01.T01 |
| Gene Sequence |
| Protein sequence |
