AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880043217.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880043217.01.T01 MTR_3g011280 85.039 127 17 1 1 127 690 814 3.39e-66 217
MsG0880043217.01.T01 MTR_3g011280 85.039 127 17 1 1 127 658 782 4.63e-66 216
MsG0880043217.01.T01 MTR_3g011390 80.952 126 22 1 1 126 692 815 7.32e-63 207
MsG0880043217.01.T01 MTR_3g011420 80.315 127 23 1 1 127 691 815 6.16e-61 202
MsG0880043217.01.T01 MTR_3g011270 70.339 118 35 0 1 118 689 806 1.67e-51 176
MsG0880043217.01.T01 MTR_0691s0020 68.644 118 37 0 1 118 690 807 2.34e-50 172
MsG0880043217.01.T01 MTR_3g011370 69.492 118 36 0 1 118 690 807 2.34e-50 172
MsG0880043217.01.T01 MTR_3g011480 77.119 118 27 0 1 118 689 806 3.47e-50 172
MsG0880043217.01.T01 MTR_3g019340 66.667 117 39 0 1 117 644 760 2.89e-49 169
MsG0880043217.01.T01 MTR_3g011460 68.644 118 37 0 1 118 689 806 4.90e-48 166
MsG0880043217.01.T01 MTR_3g011360 64.567 127 43 1 1 127 658 782 3.73e-47 163
MsG0880043217.01.T01 MTR_3g012310 57.031 128 53 1 3 130 691 816 8.06e-43 151
MsG0880043217.01.T01 MTR_1g031720 52.000 150 65 3 1 149 814 957 1.51e-37 136
MsG0880043217.01.T01 MTR_3g012060 53.043 115 54 0 3 117 687 801 4.74e-37 134
MsG0880043217.01.T01 MTR_3g011300 63.780 127 44 1 1 127 687 811 6.65e-37 134
MsG0880043217.01.T01 MTR_3g012090 56.410 117 51 0 1 117 688 804 1.13e-36 134
MsG0880043217.01.T01 MTR_3g012240 52.344 128 59 1 3 130 737 862 1.22e-35 130
MsG0880043217.01.T01 MTR_3g012250 50.388 129 61 2 3 130 696 822 2.54e-34 127
MsG0880043217.01.T01 MTR_1g016217 52.308 130 60 1 1 130 1 128 1.59e-32 116
MsG0880043217.01.T01 MTR_3g012050 51.562 128 60 1 3 130 675 800 8.09e-32 120
MsG0880043217.01.T01 MTR_1g016210 52.308 130 60 1 1 130 691 818 1.03e-30 116
MsG0880043217.01.T01 MTR_3g056585 51.639 122 55 2 1 118 668 789 2.30e-28 110
MsG0880043217.01.T01 MTR_3g013700 32.836 201 68 2 3 156 475 655 1.49e-26 104
MsG0880043217.01.T01 MTR_3g012080 48.113 106 53 1 25 130 1 104 2.84e-26 99.8
MsG0880043217.01.T01 MTR_5g027860 42.038 157 82 4 1 151 671 824 4.03e-26 103
MsG0880043217.01.T01 MTR_3g456080 43.750 144 72 3 1 141 108 245 5.73e-26 100
MsG0880043217.01.T01 MTR_3g056590 41.667 156 76 3 1 147 689 838 1.28e-24 99.4
MsG0880043217.01.T01 MTR_5g027810 44.068 118 65 1 1 117 676 793 1.33e-24 99.4
MsG0880043217.01.T01 MTR_3g055930 43.750 128 69 2 1 127 700 825 1.75e-24 99.0
MsG0880043217.01.T01 MTR_3g012080 47.059 102 52 1 29 130 2 101 2.79e-24 94.4
MsG0880043217.01.T01 MTR_1g078150 41.722 151 78 4 1 147 480 624 5.61e-24 97.4
MsG0880043217.01.T01 MTR_5g027900 44.068 118 65 1 1 117 673 790 7.81e-24 97.1
MsG0880043217.01.T01 MTR_3g012270 51.685 89 41 1 1 87 693 781 1.30e-23 96.3
MsG0880043217.01.T01 MTR_1g078190 42.105 152 77 5 1 147 689 834 3.10e-22 92.4
MsG0880043217.01.T01 MTR_3g069210 45.455 121 63 2 1 118 667 787 4.13e-22 92.0
MsG0880043217.01.T01 MTR_3g056625 40.164 122 69 1 1 118 366 487 6.28e-22 91.7
MsG0880043217.01.T01 MTR_5g027910 43.966 116 64 1 3 117 678 793 1.10e-21 90.9
MsG0880043217.01.T01 MTR_5g027870 44.828 116 63 1 3 117 676 791 1.39e-21 90.5
MsG0880043217.01.T01 MTR_1g078170 44.144 111 58 1 1 107 188 298 3.47e-21 89.4
MsG0880043217.01.T01 MTR_3g056360 42.308 130 70 2 1 127 650 777 4.86e-20 86.3
MsG0880043217.01.T01 MTR_3g056410 42.308 130 70 2 1 127 641 768 5.29e-20 86.3
MsG0880043217.01.T01 MTR_5g092950 44.262 122 64 2 1 118 237 358 1.18e-19 84.7
MsG0880043217.01.T01 MTR_3g056620 42.342 111 62 1 17 127 722 830 1.96e-19 84.3
MsG0880043217.01.T01 MTR_1g011990 42.623 122 65 2 1 118 607 727 1.43e-18 82.0
MsG0880043217.01.T01 MTR_5g074530 57.812 64 26 1 16 79 51 113 2.73e-18 75.9
MsG0880043217.01.T01 MTR_3g056200 40.310 129 69 2 1 121 678 806 5.11e-18 80.5
MsG0880043217.01.T01 MTR_3g056320 39.370 127 69 2 1 119 683 809 2.46e-17 78.6
MsG0880043217.01.T01 MTR_3g055740 38.333 120 72 2 1 118 615 734 1.66e-15 73.2
MsG0880043217.01.T01 MTR_3g055830 37.815 119 73 1 1 118 671 789 2.11e-15 72.8
MsG0880043217.01.T01 MTR_3g455940 37.815 119 73 1 1 118 528 646 2.33e-15 72.8
MsG0880043217.01.T01 MTR_3g056310 38.281 128 72 1 1 121 680 807 5.39e-15 71.6
MsG0880043217.01.T01 MTR_3g056380 38.281 128 69 2 1 121 676 800 7.13e-15 71.2
MsG0880043217.01.T01 MTR_3g055720 36.667 120 72 2 1 118 674 791 1.14e-14 70.9
MsG0880043217.01.T01 MTR_3g056190 36.090 133 73 3 1 121 688 820 1.24e-14 70.9
MsG0880043217.01.T01 MTR_3g456030 35.294 119 76 1 1 118 673 791 5.07e-13 66.2
MsG0880043217.01.T01 MTR_3g456070 33.613 119 78 1 1 118 517 635 2.36e-12 63.9
MsG0880043217.01.T01 MTR_3g455910 33.613 119 78 1 1 118 597 715 5.01e-12 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 34 sgRNAs with CRISPR-Local

Find 56 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTTAAGGACGTTAGGTTTA+AGG 0.196607 8:-20803005 MsG0880043217.01.T01:CDS
TCAACAAGATACTCAAGTTT+TGG 0.284159 8:+20802799 None:intergenic
CTGAGGGTACCTCCTTCAGC+TGG 0.335878 8:+20802537 None:intergenic
CAATTGAAGCCGCTGAAGTT+GGG 0.346403 8:+20802855 None:intergenic
TATTATGTTGAGGTAAATCA+TGG 0.391171 8:-20803066 MsG0880043217.01.T01:CDS
TTCTACTACAACAGCACTTA+AGG 0.404293 8:+20802957 None:intergenic
AATACTTTCAACAAATCTTC+TGG 0.408565 8:+20802467 None:intergenic
TGAGGGTACCTCCTTCAGCT+GGG 0.431137 8:+20802538 None:intergenic
AACTAGATATATTGCCTGAA+TGG 0.436563 8:-20802825 MsG0880043217.01.T01:CDS
TCAATTGAAGCCGCTGAAGT+TGG 0.442838 8:+20802854 None:intergenic
TGAAAATGTTAAGTCAGTTA+AGG 0.443456 8:-20803020 MsG0880043217.01.T01:CDS
GATCTGAATATCACTACAAT+TGG 0.446557 8:-20802919 MsG0880043217.01.T01:CDS
ACCCTCAGGCATCAGGTCCT+TGG 0.453611 8:-20802522 MsG0880043217.01.T01:CDS
TTAAGTCAGTTAAGGACGTT+AGG 0.458090 8:-20803012 MsG0880043217.01.T01:CDS
AGGAGGTACCCTCAGGCATC+AGG 0.476710 8:-20802529 MsG0880043217.01.T01:CDS
CAGCTGAAGGAGGTACCCTC+AGG 0.492598 8:-20802536 MsG0880043217.01.T01:CDS
GAGGGTACCTCCTTCAGCTG+GGG 0.518269 8:+20802539 None:intergenic
TAGTGATATTCAGATCTTCA+AGG 0.533215 8:+20802926 None:intergenic
GATAACTTTGAGATTATCCA+AGG 0.542505 8:+20802505 None:intergenic
AGGGTACCTCCTTCAGCTGG+GGG 0.546435 8:+20802540 None:intergenic
CTTCAGCGGCTTCAATTGAA+AGG 0.549966 8:-20802850 MsG0880043217.01.T01:CDS
ATTATGTTGAGGTAAATCAT+GGG 0.551874 8:-20803065 MsG0880043217.01.T01:CDS
AATTGAAGCCGCTGAAGTTG+GGG 0.554713 8:+20802856 None:intergenic
GAAAGTATTGATCCAGACAA+AGG 0.596444 8:-20802453 MsG0880043217.01.T01:CDS
TTATGTTGAGGTAAATCATG+GGG 0.597922 8:-20803064 MsG0880043217.01.T01:CDS
CATCTCCACCCCAACTTCAG+CGG 0.598929 8:-20802864 MsG0880043217.01.T01:CDS
AGGCATGTCAGAAGAGAACA+TGG 0.599895 8:-20802985 MsG0880043217.01.T01:CDS
TGAAGCCGCTGAAGTTGGGG+TGG 0.611493 8:+20802859 None:intergenic
TCCAAGGACCTGATGCCTGA+GGG 0.617728 8:+20802521 None:intergenic
ATCCAAGGACCTGATGCCTG+AGG 0.631925 8:+20802520 None:intergenic
GAATATCACTACAATTGGTG+AGG 0.635087 8:-20802914 MsG0880043217.01.T01:CDS
GCATGTCAGAAGAGAACATG+GGG 0.672397 8:-20802983 MsG0880043217.01.T01:CDS
TATGTTGAGGTAAATCATGG+GGG 0.674072 8:-20803063 MsG0880043217.01.T01:CDS
GGCATGTCAGAAGAGAACAT+GGG 0.704208 8:-20802984 MsG0880043217.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AACATAATAAAGTTTTTGTA+GGG + Chr8:20802449-20802468 None:intergenic 15.0%
!! ACAAAAACTTTATTATGTTG+AGG - Chr8:20802450-20802469 MsG0880043217.01.T01:CDS 20.0%
!!! CAACATAATAAAGTTTTTGT+AGG + Chr8:20802450-20802469 None:intergenic 20.0%
!!! GAAATTTTGCATTTTCAAAT+TGG - Chr8:20802853-20802872 MsG0880043217.01.T01:CDS 20.0%
!!! TGAGAAAAAATTCAAGTTAA+TGG + Chr8:20802644-20802663 None:intergenic 20.0%
! AATACTTTCAACAAATCTTC+TGG + Chr8:20803062-20803081 None:intergenic 25.0%
! ATTATGTTGAGGTAAATCAT+GGG - Chr8:20802461-20802480 MsG0880043217.01.T01:CDS 25.0%
! TATTATGTTGAGGTAAATCA+TGG - Chr8:20802460-20802479 MsG0880043217.01.T01:CDS 25.0%
! TGAAAATGTTAAGTCAGTTA+AGG - Chr8:20802506-20802525 MsG0880043217.01.T01:CDS 25.0%
!! ATTTTGCATTTTCAAATTGG+AGG - Chr8:20802856-20802875 MsG0880043217.01.T01:CDS 25.0%
AACTAGATATATTGCCTGAA+TGG - Chr8:20802701-20802720 MsG0880043217.01.T01:intron 30.0%
AGATCGAAATTCAACAGATT+TGG + Chr8:20802810-20802829 None:intergenic 30.0%
ATCAAAGTTCATCGTCTATA+AGG + Chr8:20802936-20802955 None:intergenic 30.0%
ATCTGTTGAATTTCGATCTA+TGG - Chr8:20802811-20802830 MsG0880043217.01.T01:CDS 30.0%
ATGCAAAATTTCACCATCAT+AGG + Chr8:20802845-20802864 None:intergenic 30.0%
GATCTGAATATCACTACAAT+TGG - Chr8:20802607-20802626 MsG0880043217.01.T01:intron 30.0%
TAGTGATATTCAGATCTTCA+AGG + Chr8:20802603-20802622 None:intergenic 30.0%
TCTGTTGAATTTCGATCTAT+GGG - Chr8:20802812-20802831 MsG0880043217.01.T01:CDS 30.0%
TTATGTTGAGGTAAATCATG+GGG - Chr8:20802462-20802481 MsG0880043217.01.T01:CDS 30.0%
! TCAACAAGATACTCAAGTTT+TGG + Chr8:20802730-20802749 None:intergenic 30.0%
!! GATAACTTTGAGATTATCCA+AGG + Chr8:20803024-20803043 None:intergenic 30.0%
AGTTAAGGACGTTAGGTTTA+AGG - Chr8:20802521-20802540 MsG0880043217.01.T01:CDS 35.0%
CTCTCTTGAATATCTTACCA+TGG - Chr8:20802956-20802975 MsG0880043217.01.T01:CDS 35.0%
GAATATCACTACAATTGGTG+AGG - Chr8:20802612-20802631 MsG0880043217.01.T01:intron 35.0%
TATGTTGAGGTAAATCATGG+GGG - Chr8:20802463-20802482 MsG0880043217.01.T01:CDS 35.0%
TTAAGTCAGTTAAGGACGTT+AGG - Chr8:20802514-20802533 MsG0880043217.01.T01:CDS 35.0%
TTCTACTACAACAGCACTTA+AGG + Chr8:20802572-20802591 None:intergenic 35.0%
! AAGTAGTCTTTTCCTTTGTC+TGG + Chr8:20803088-20803107 None:intergenic 35.0%
!! GAAAGTATTGATCCAGACAA+AGG - Chr8:20803073-20803092 MsG0880043217.01.T01:CDS 35.0%
!!! TCAAGTTTTGGAATCCATTC+AGG + Chr8:20802718-20802737 None:intergenic 35.0%
TATGGGATGATGTCCTATGA+TGG - Chr8:20802829-20802848 MsG0880043217.01.T01:CDS 40.0%
!!! CAAGTTTTTAGTGACTGCAG+TGG + Chr8:20802787-20802806 None:intergenic 40.0%
AATTGAAGCCGCTGAAGTTG+GGG + Chr8:20802673-20802692 None:intergenic 45.0%
AGGCATGTCAGAAGAGAACA+TGG - Chr8:20802541-20802560 MsG0880043217.01.T01:CDS 45.0%
CAATTGAAGCCGCTGAAGTT+GGG + Chr8:20802674-20802693 None:intergenic 45.0%
CTTCAGCGGCTTCAATTGAA+AGG - Chr8:20802676-20802695 MsG0880043217.01.T01:intron 45.0%
GAACCTCACTCGCTTGAATA+GGG - Chr8:20802900-20802919 MsG0880043217.01.T01:CDS 45.0%
GCATGTCAGAAGAGAACATG+GGG - Chr8:20802543-20802562 MsG0880043217.01.T01:CDS 45.0%
GGCATGTCAGAAGAGAACAT+GGG - Chr8:20802542-20802561 MsG0880043217.01.T01:CDS 45.0%
TCAATTGAAGCCGCTGAAGT+TGG + Chr8:20802675-20802694 None:intergenic 45.0%
TGAACCTCACTCGCTTGAAT+AGG - Chr8:20802899-20802918 MsG0880043217.01.T01:CDS 45.0%
TTACCCTATTCAAGCGAGTG+AGG + Chr8:20802906-20802925 None:intergenic 45.0%
ATCCAAGGACCTGATGCCTG+AGG + Chr8:20803009-20803028 None:intergenic 55.0%
CATCTCCACCCCAACTTCAG+CGG - Chr8:20802662-20802681 MsG0880043217.01.T01:intron 55.0%
TCCAAGGACCTGATGCCTGA+GGG + Chr8:20803008-20803027 None:intergenic 55.0%
!! TGAGGGTACCTCCTTCAGCT+GGG + Chr8:20802991-20803010 None:intergenic 55.0%
ACCCTCAGGCATCAGGTCCT+TGG - Chr8:20803004-20803023 MsG0880043217.01.T01:CDS 60.0%
CAGAATCCCCCAGCTGAAGG+AGG - Chr8:20802980-20802999 MsG0880043217.01.T01:CDS 60.0%
CAGCTGGGGGATTCTGTCCA+TGG + Chr8:20802976-20802995 None:intergenic 60.0%
GGACAGAATCCCCCAGCTGA+AGG - Chr8:20802977-20802996 MsG0880043217.01.T01:CDS 60.0%
TGAAGCCGCTGAAGTTGGGG+TGG + Chr8:20802670-20802689 None:intergenic 60.0%
! AGGGTACCTCCTTCAGCTGG+GGG + Chr8:20802989-20803008 None:intergenic 60.0%
!! AGGAGGTACCCTCAGGCATC+AGG - Chr8:20802997-20803016 MsG0880043217.01.T01:CDS 60.0%
!! CAGCTGAAGGAGGTACCCTC+AGG - Chr8:20802990-20803009 MsG0880043217.01.T01:CDS 60.0%
!! CTGAGGGTACCTCCTTCAGC+TGG + Chr8:20802992-20803011 None:intergenic 60.0%
!! GAGGGTACCTCCTTCAGCTG+GGG + Chr8:20802990-20803009 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr8 gene 20802439 20803109 20802439 ID=MsG0880043217.01;Name=MsG0880043217.01
Chr8 mRNA 20802439 20803109 20802439 ID=MsG0880043217.01.T01;Parent=MsG0880043217.01;Name=MsG0880043217.01.T01;_AED=0.50;_eAED=0.52;_QI=0|0|0|1|1|1|2|0|159
Chr8 exon 20802757 20803109 20802757 ID=MsG0880043217.01.T01:exon:4232;Parent=MsG0880043217.01.T01
Chr8 exon 20802439 20802565 20802439 ID=MsG0880043217.01.T01:exon:4231;Parent=MsG0880043217.01.T01
Chr8 CDS 20802757 20803109 20802757 ID=MsG0880043217.01.T01:cds;Parent=MsG0880043217.01.T01
Chr8 CDS 20802439 20802565 20802439 ID=MsG0880043217.01.T01:cds;Parent=MsG0880043217.01.T01
Gene Sequence

>MsG0880043217.01.T01

ATGGTTTCCCTACAAAAACTTTATTATGTTGAGGTAAATCATGGGGGAGTAGATCTCATTCAAGAGATGAAAATGTTAAGTCAGTTAAGGACGTTAGGTTTAAGGCATGTCAGAAGAGAACATGGGGATGCCTTAAGTGCTGTTGTAGTAGAATTGAAACACCTTGAAGATCTGAATATCACTACAATTGGTGAGGATGAAACCATTAACTTGAATTTTTTCTCATCTCCACCCCAACTTCAGCGGCTTCAATTGAAAGGTAAACTAGATATATTGCCTGAATGGATTCCAAAACTTGAGTATCTTGTTGAGATAAAGTTAGCTTTATCTAAGTTGACAAATGATCCACTGCAAATCCCCCAGCTGAAGGAGGTACCCTCAGGCATCAGGTCCTTGGATAATCTCAAAGTTATCAACTTCGCTGAAATGCCAGAAGATTTGTTGAAAGTATTGATCCAGACAAAGGAAAAGACTACTTGA

Protein sequence

>MsG0880043217.01.T01

MVSLQKLYYVEVNHGGVDLIQEMKMLSQLRTLGLRHVRREHGDALSAVVVELKHLEDLNITTIGEDETINLNFFSSPPQLQRLQLKGKLDILPEWIPKLEYLVEIKLALSKLTNDPLQIPQLKEVPSGIRSLDNLKVINFAEMPEDLLKVLIQTKEKTT*