AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780041248.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780041248.01.T01 MTR_7g107500 95.062 81 4 0 1 81 17 97 9.35e-53 159
MsG0780041248.01.T01 MTR_7g107520 98.765 81 1 0 1 81 240 320 3.74e-52 165
MsG0780041248.01.T01 MTR_4g046713 48.780 82 41 1 1 81 245 326 2.15e-20 82.8
MsG0780041248.01.T01 MTR_1g098320 50.000 78 38 1 5 81 47 124 5.10e-18 73.2
MsG0780041248.01.T01 MTR_2g088770 42.169 83 46 1 1 81 243 325 2.44e-17 74.3
MsG0780041248.01.T01 MTR_1g009750 46.341 82 43 1 1 81 230 311 1.76e-16 72.0
MsG0780041248.01.T01 MTR_1g009750 46.341 82 43 1 1 81 247 328 1.84e-16 72.0
MsG0780041248.01.T01 MTR_3g094650 46.154 78 41 1 1 77 241 318 3.45e-16 71.2
MsG0780041248.01.T01 MTR_6g043280 43.902 82 45 1 1 81 251 332 6.06e-16 70.5
MsG0780041248.01.T01 MTR_5g017860 46.341 82 43 1 1 81 245 326 7.31e-16 70.5
MsG0780041248.01.T01 MTR_4g095450 46.914 81 42 1 1 80 243 323 8.28e-16 70.1
MsG0780041248.01.T01 MTR_5g021060 49.333 75 36 2 9 81 256 330 1.42e-15 69.7
MsG0780041248.01.T01 MTR_5g022870 44.048 84 37 4 1 81 240 316 4.47e-15 68.2
MsG0780041248.01.T01 MTR_8g075100 45.000 80 35 3 5 81 246 319 8.24e-15 67.4
MsG0780041248.01.T01 MTR_4g125940 44.186 86 43 2 1 81 189 274 8.75e-15 67.0
MsG0780041248.01.T01 MTR_1g054205 45.238 84 43 2 1 81 243 326 1.22e-14 67.0
MsG0780041248.01.T01 MTR_7g029030 43.902 82 45 1 1 81 239 320 1.26e-14 67.0
MsG0780041248.01.T01 MTR_4g125940 41.860 86 45 1 1 81 244 329 1.45e-14 67.0
MsG0780041248.01.T01 MTR_5g016010 43.210 81 45 1 1 80 248 328 1.49e-14 66.6
MsG0780041248.01.T01 MTR_7g093370 45.238 84 43 2 1 81 288 371 1.50e-14 67.0
MsG0780041248.01.T01 MTR_2g029860 46.429 84 42 2 1 81 247 330 1.63e-14 66.6
MsG0780041248.01.T01 MTR_2g029830 44.578 83 43 2 2 81 179 261 1.65e-14 66.2
MsG0780041248.01.T01 MTR_3g092990 45.122 82 44 1 1 81 234 315 2.09e-14 66.2
MsG0780041248.01.T01 MTR_3g092990 45.122 82 44 1 1 81 245 326 2.20e-14 66.2
MsG0780041248.01.T01 MTR_2g029830 44.578 83 43 2 2 81 247 329 2.52e-14 66.2
MsG0780041248.01.T01 MTR_2g029820 44.578 83 43 2 2 81 248 330 5.46e-14 65.5
MsG0780041248.01.T01 MTR_2g029740 44.578 83 43 2 2 81 248 330 5.85e-14 65.1
MsG0780041248.01.T01 MTR_2g084090 40.244 82 48 1 1 81 237 318 9.80e-14 64.3
MsG0780041248.01.T01 MTR_6g043460 40.244 82 48 1 1 81 247 328 1.15e-13 64.3
MsG0780041248.01.T01 MTR_2g029800 43.373 83 44 2 2 81 248 330 1.22e-13 64.3
MsG0780041248.01.T01 MTR_1g086320 40.244 82 48 1 1 81 252 333 1.44e-13 63.9
MsG0780041248.01.T01 MTR_6g043240 41.463 82 47 1 1 81 250 331 1.55e-13 63.9
MsG0780041248.01.T01 MTR_2g040000 42.045 88 43 3 1 81 245 331 1.62e-13 63.9
MsG0780041248.01.T01 MTR_4g127670 43.902 82 45 1 1 81 242 323 1.70e-13 63.9
MsG0780041248.01.T01 MTR_2g084080 40.244 82 48 1 1 81 236 317 1.76e-13 63.9
MsG0780041248.01.T01 MTR_7g072510 40.244 82 48 1 1 81 231 312 1.87e-13 63.5
MsG0780041248.01.T01 MTR_1g038680 37.805 82 50 1 1 81 251 332 2.23e-13 63.5
MsG0780041248.01.T01 MTR_1g077000 40.541 74 43 1 9 81 254 327 2.37e-13 63.5
MsG0780041248.01.T01 MTR_5g017850 40.244 82 48 1 1 81 245 326 2.69e-13 63.2
MsG0780041248.01.T01 MTR_2g029750 45.783 83 42 2 2 81 248 330 2.79e-13 63.2
MsG0780041248.01.T01 MTR_2g029850 45.238 84 43 2 1 81 247 330 2.83e-13 63.2
MsG0780041248.01.T01 MTR_2g029815 43.373 83 44 2 2 81 248 330 3.19e-13 63.2
MsG0780041248.01.T01 MTR_8g076820 44.578 83 44 2 1 81 248 330 3.31e-13 63.2
MsG0780041248.01.T01 MTR_3g094670 41.026 78 45 1 1 77 241 318 3.51e-13 62.8
MsG0780041248.01.T01 MTR_0155s0040 38.462 78 47 1 5 81 238 315 5.80e-13 62.4
MsG0780041248.01.T01 MTR_2g029910 44.048 84 44 2 1 81 249 332 5.96e-13 62.4
MsG0780041248.01.T01 MTR_5g083860 40.244 82 48 1 1 81 244 325 6.44e-13 62.4
MsG0780041248.01.T01 MTR_2g084020 43.373 83 45 1 1 81 236 318 6.48e-13 62.0
MsG0780041248.01.T01 MTR_2g028980 42.169 83 45 2 2 81 248 330 7.65e-13 62.0
MsG0780041248.01.T01 MTR_3g072190 39.024 82 49 1 1 81 170 251 8.39e-13 61.2
MsG0780041248.01.T01 MTR_2g084110 40.244 82 48 1 1 81 236 317 9.34e-13 61.6
MsG0780041248.01.T01 MTR_1g101830 41.772 79 43 2 4 81 246 322 9.89e-13 61.6
MsG0780041248.01.T01 MTR_4g132110 39.506 81 48 1 2 81 248 328 2.60e-12 60.5
MsG0780041248.01.T01 MTR_5g014100 37.647 85 49 1 1 81 247 331 2.66e-12 60.5
MsG0780041248.01.T01 MTR_4g132110 39.506 81 48 1 2 81 253 333 2.96e-12 60.5
MsG0780041248.01.T01 MTR_3g072190 39.024 82 49 1 1 81 237 318 3.26e-12 60.1
MsG0780041248.01.T01 MTR_3g105790 39.024 82 49 1 1 81 165 246 3.50e-12 59.7
MsG0780041248.01.T01 MTR_3g105790 39.024 82 49 1 1 81 241 322 5.39e-12 59.7
MsG0780041248.01.T01 MTR_2g084010 43.373 83 45 1 1 81 236 318 7.50e-12 59.3
MsG0780041248.01.T01 MTR_2g084000 40.244 82 47 2 2 81 238 319 8.39e-12 58.9
MsG0780041248.01.T01 MTR_3g094630 39.024 82 49 1 1 81 241 322 2.02e-11 58.2
MsG0780041248.01.T01 MTR_7g086820 36.585 82 51 1 1 81 231 312 3.61e-11 57.4
MsG0780041248.01.T01 MTR_1g086490 38.272 81 49 1 2 81 260 340 3.83e-11 57.4
MsG0780041248.01.T01 MTR_1g066710 37.037 81 50 1 2 81 243 323 4.49e-11 57.0
MsG0780041248.01.T01 MTR_7g026990 40.506 79 46 1 4 81 244 322 6.32e-11 56.6
MsG0780041248.01.T01 MTR_2g029560 41.667 84 46 2 1 81 242 325 8.26e-11 56.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780041248.01.T01 AT3G21770 47.561 82 41 1 1 80 246 327 5.66e-20 81.6
MsG0780041248.01.T01 AT4G11290 49.398 83 40 1 1 81 244 326 4.50e-19 79.3
MsG0780041248.01.T01 AT1G05260 46.988 83 42 1 1 81 243 325 5.93e-19 79.0
MsG0780041248.01.T01 AT4G30170 50.000 82 40 1 1 81 244 325 2.20e-18 77.4
MsG0780041248.01.T01 AT1G05240 43.529 85 44 1 1 81 241 325 6.48e-18 75.9
MsG0780041248.01.T01 AT1G05250 43.529 85 44 1 1 81 241 325 6.48e-18 75.9
MsG0780041248.01.T01 AT5G15180 45.783 83 43 1 1 81 247 329 9.44e-18 75.5
MsG0780041248.01.T01 AT2G24800 46.914 81 42 1 2 81 249 329 9.53e-18 75.5
MsG0780041248.01.T01 AT2G39040 45.783 83 43 1 1 81 268 350 2.70e-17 74.7
MsG0780041248.01.T01 AT5G19880 45.238 84 43 2 1 81 246 329 4.64e-17 73.6
MsG0780041248.01.T01 AT2G18140 44.578 83 44 2 1 81 253 335 6.67e-17 73.2
MsG0780041248.01.T01 AT4G31760 44.444 81 44 1 2 81 246 326 7.26e-17 73.2
MsG0780041248.01.T01 AT2G18150 43.373 83 45 2 1 81 254 336 7.75e-16 70.5
MsG0780041248.01.T01 AT4G37530 45.122 82 44 1 1 81 248 329 9.06e-16 70.1
MsG0780041248.01.T01 AT5G39580 43.902 82 45 1 1 81 238 319 1.03e-15 70.1
MsG0780041248.01.T01 AT5G64120 45.122 82 44 1 1 81 247 328 1.12e-15 70.1
MsG0780041248.01.T01 AT2G18980 45.122 82 44 1 1 81 242 323 1.30e-15 69.7
MsG0780041248.01.T01 AT4G36430 42.169 83 46 2 1 81 248 330 1.56e-15 69.7
MsG0780041248.01.T01 AT1G71695 45.679 81 43 1 2 81 259 339 1.69e-15 69.7
MsG0780041248.01.T01 AT4G37520 43.902 82 45 1 1 81 245 326 2.49e-15 68.9
MsG0780041248.01.T01 AT4G37520 43.902 82 45 1 1 81 248 329 2.71e-15 68.9
MsG0780041248.01.T01 AT1G14540 46.914 81 38 2 3 81 238 315 3.44e-15 68.6
MsG0780041248.01.T01 AT3G01190 43.373 83 45 1 1 81 239 321 4.44e-15 68.2
MsG0780041248.01.T01 AT1G14550 46.914 81 38 2 3 81 244 321 6.85e-15 67.8
MsG0780041248.01.T01 AT2G35380 40.000 85 47 1 1 81 163 247 1.12e-14 66.6
MsG0780041248.01.T01 AT5G66390 50.000 76 34 3 9 81 259 333 1.62e-14 66.6
MsG0780041248.01.T01 AT2G35380 40.000 85 47 1 1 81 251 335 2.14e-14 66.6
MsG0780041248.01.T01 AT5G17820 39.024 82 49 1 1 81 232 313 3.64e-14 65.9
MsG0780041248.01.T01 AT5G17820 39.024 82 49 1 1 81 285 366 4.67e-14 65.5
MsG0780041248.01.T01 AT3G49960 43.902 82 45 1 1 81 248 329 5.22e-14 65.5
MsG0780041248.01.T01 AT5G14130 42.683 82 46 1 1 81 290 371 1.06e-13 64.7
MsG0780041248.01.T01 AT5G14130 42.683 82 46 1 1 81 249 330 1.09e-13 64.3
MsG0780041248.01.T01 AT5G64110 42.353 85 45 1 1 81 246 330 2.08e-13 63.5
MsG0780041248.01.T01 AT4G08770 42.857 84 44 2 2 81 244 327 2.60e-13 63.5
MsG0780041248.01.T01 AT5G06730 45.238 84 43 2 1 81 251 334 2.69e-13 63.5
MsG0780041248.01.T01 AT5G67400 41.463 82 47 1 1 81 248 329 3.21e-13 63.2
MsG0780041248.01.T01 AT1G34510 37.805 82 50 1 1 81 228 309 4.11e-13 62.8
MsG0780041248.01.T01 AT2G38390 45.238 84 43 2 1 81 250 333 4.65e-13 62.8
MsG0780041248.01.T01 AT5G19890 39.535 86 45 2 1 81 244 327 5.15e-13 62.4
MsG0780041248.01.T01 AT5G06720 45.238 84 43 2 1 81 250 333 1.14e-12 61.6
MsG0780041248.01.T01 AT4G33420 39.189 74 44 1 9 81 252 325 2.63e-12 60.5
MsG0780041248.01.T01 AT4G33420 39.189 74 44 1 9 81 299 372 2.92e-12 60.5
MsG0780041248.01.T01 AT4G21960 37.037 81 50 1 2 81 247 327 6.13e-12 59.7
MsG0780041248.01.T01 AT3G50990 40.964 83 47 1 1 81 259 341 7.17e-12 59.3
MsG0780041248.01.T01 AT3G32980 40.000 85 45 2 2 81 251 334 1.31e-11 58.5
MsG0780041248.01.T01 AT5G24070 40.741 81 47 1 2 81 255 335 1.35e-11 58.5
MsG0780041248.01.T01 AT5G40150 38.554 83 45 3 1 81 250 328 3.09e-11 57.8
MsG0780041248.01.T01 AT5G58390 38.462 78 47 1 1 77 235 312 4.48e-11 57.0
MsG0780041248.01.T01 AT3G28200 36.585 82 48 2 1 81 238 316 5.46e-11 57.0
MsG0780041248.01.T01 AT4G08780 40.000 85 45 2 2 81 244 327 6.99e-11 56.6

Find 14 sgRNAs with CRISPR-Local

Find 16 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAATTCATTAACAATGTTTC+TGG 0.127160 7:+88773738 None:intergenic
TCAAATGTTGTTGAGCTATT+TGG 0.173041 7:+88773838 None:intergenic
CAGAAACATTGTTAATGAAT+TGG 0.289764 7:-88773737 MsG0780041248.01.T01:CDS
GTTTGAATGGAGAAATTAGA+AGG 0.402528 7:-88773642 MsG0780041248.01.T01:CDS
GAGGTCCTCACTGGTTTGAA+TGG 0.449923 7:-88773655 MsG0780041248.01.T01:CDS
AACAATGTTTCTGGATTGCT+TGG 0.462159 7:+88773747 None:intergenic
GAAGAGGATGGGAGCTATTG+AGG 0.498561 7:-88773674 MsG0780041248.01.T01:CDS
GGAGCTATTGAGGTCCTCAC+TGG 0.501455 7:-88773664 MsG0780041248.01.T01:CDS
TTCTCAGTCAATGAAGAGGA+TGG 0.515611 7:-88773686 MsG0780041248.01.T01:CDS
TCCTGTACTTCTACAAAACA+AGG 0.536222 7:-88773803 MsG0780041248.01.T01:CDS
CTTCATTGACTGAGAAAACT+CGG 0.554984 7:+88773693 None:intergenic
TCTCAGTCAATGAAGAGGAT+GGG 0.577113 7:-88773685 MsG0780041248.01.T01:CDS
CCTGTACTTCTACAAAACAA+GGG 0.691319 7:-88773802 MsG0780041248.01.T01:CDS
TTTCTCCATTCAAACCAGTG+AGG 0.718240 7:+88773650 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! CAATTCATTAACAATGTTTC+TGG + Chr7:88773730-88773749 None:intergenic 25.0%
! CAGAAACATTGTTAATGAAT+TGG - Chr7:88773728-88773747 MsG0780041248.01.T01:CDS 25.0%
GTTTGAATGGAGAAATTAGA+AGG - Chr7:88773823-88773842 MsG0780041248.01.T01:CDS 30.0%
TCAAATGTTGTTGAGCTATT+TGG + Chr7:88773630-88773649 None:intergenic 30.0%
CCTGTACTTCTACAAAACAA+GGG - Chr7:88773663-88773682 MsG0780041248.01.T01:CDS 35.0%
TCCTGTACTTCTACAAAACA+AGG - Chr7:88773662-88773681 MsG0780041248.01.T01:CDS 35.0%
! AGTTTTCTCAGTCAATGAAG+AGG - Chr7:88773775-88773794 MsG0780041248.01.T01:CDS 35.0%
! CTTCATTGACTGAGAAAACT+CGG + Chr7:88773775-88773794 None:intergenic 35.0%
!! AACAATGTTTCTGGATTGCT+TGG + Chr7:88773721-88773740 None:intergenic 35.0%
TCTCAGTCAATGAAGAGGAT+GGG - Chr7:88773780-88773799 MsG0780041248.01.T01:CDS 40.0%
TTCTCAGTCAATGAAGAGGA+TGG - Chr7:88773779-88773798 MsG0780041248.01.T01:CDS 40.0%
TTTCTCCATTCAAACCAGTG+AGG + Chr7:88773818-88773837 None:intergenic 40.0%
!!! CCCTTGTTTTGTAGAAGTAC+AGG + Chr7:88773666-88773685 None:intergenic 40.0%
GAAGAGGATGGGAGCTATTG+AGG - Chr7:88773791-88773810 MsG0780041248.01.T01:CDS 50.0%
!! GAGGTCCTCACTGGTTTGAA+TGG - Chr7:88773810-88773829 MsG0780041248.01.T01:CDS 50.0%
GGAGCTATTGAGGTCCTCAC+TGG - Chr7:88773801-88773820 MsG0780041248.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr7 gene 88773621 88773866 88773621 ID=MsG0780041248.01;Name=MsG0780041248.01
Chr7 mRNA 88773621 88773866 88773621 ID=MsG0780041248.01.T01;Parent=MsG0780041248.01;Name=MsG0780041248.01.T01;_AED=0.40;_eAED=0.40;_QI=0|-1|0|1|-1|0|1|0|81
Chr7 exon 88773621 88773866 88773621 ID=MsG0780041248.01.T01:exon:20222;Parent=MsG0780041248.01.T01
Chr7 CDS 88773621 88773866 88773621 ID=MsG0780041248.01.T01:cds;Parent=MsG0780041248.01.T01
Gene Sequence

>MsG0780041248.01.T01

ATGGATCCAAATAGCTCAACAACATTTGACAATGACTATTATCCTGTACTTCTACAAAACAAGGGTCTCTTCACATCAGACGCTGCTCTTCTAACAACCAAGCAATCCAGAAACATTGTTAATGAATTGGTTTCACAAAACAAATTCTTCACCGAGTTTTCTCAGTCAATGAAGAGGATGGGAGCTATTGAGGTCCTCACTGGTTTGAATGGAGAAATTAGAAGGAAGTGCTCTGTTGTTAATTAA

Protein sequence

>MsG0780041248.01.T01

MDPNSSTTFDNDYYPVLLQNKGLFTSDAALLTTKQSRNIVNELVSQNKFFTEFSQSMKRMGAIEVLTGLNGEIRRKCSVVN*