Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780038403.01.T01 | XP_013448534.1 | 94.037 | 218 | 10 | 2 | 1 | 216 | 1 | 217 | 1.41E-134 | 386 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780038403.01.T01 | B3EER1 | 45.312 | 128 | 59 | 3 | 86 | 213 | 6 | 122 | 4.00E-18 | 80.1 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780038403.01.T01 | I3SW10 | 94.037 | 218 | 10 | 2 | 1 | 216 | 1 | 217 | 6.73e-135 | 386 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048856.01 | MsG0780038403.01 | 0.814773 | 1.298567e-51 | 8.792127e-49 |
| MsG0180000139.01 | MsG0780038403.01 | 0.849479 | 3.150259e-60 | 5.979492e-57 |
| MsG0180004152.01 | MsG0780038403.01 | 0.810774 | 9.753245e-51 | 5.932210e-48 |
| MsG0180004481.01 | MsG0780038403.01 | 0.835558 | 1.564885e-56 | 1.920380e-53 |
| MsG0180004482.01 | MsG0780038403.01 | 0.836246 | 1.047409e-56 | 1.312568e-53 |
| MsG0180004509.01 | MsG0780038403.01 | 0.800896 | 1.162136e-48 | 5.474766e-46 |
| MsG0280010776.01 | MsG0780038403.01 | 0.808678 | 2.751810e-50 | 1.583260e-47 |
| MsG0480018477.01 | MsG0780038403.01 | 0.801429 | 9.042325e-49 | 4.317245e-46 |
| MsG0480018894.01 | MsG0780038403.01 | 0.801923 | 7.161324e-49 | 3.462180e-46 |
| MsG0480021006.01 | MsG0780038403.01 | 0.812151 | 4.897025e-51 | 3.089699e-48 |
| MsG0480023221.01 | MsG0780038403.01 | 0.803250 | 3.813369e-49 | 1.907034e-46 |
| MsG0580024197.01 | MsG0780038403.01 | 0.806155 | 9.435933e-50 | 5.084841e-47 |
| MsG0580024544.01 | MsG0780038403.01 | 0.806762 | 7.028914e-50 | 3.846816e-47 |
| MsG0580025651.01 | MsG0780038403.01 | 0.811153 | 8.071236e-51 | 4.958712e-48 |
| MsG0580026226.01 | MsG0780038403.01 | 0.802956 | 4.385149e-49 | 2.176581e-46 |
| MsG0280006993.01 | MsG0780038403.01 | 0.801639 | 8.189744e-49 | 3.930906e-46 |
| MsG0280007527.01 | MsG0780038403.01 | 0.826826 | 2.191833e-54 | 2.078448e-51 |
| MsG0780036240.01 | MsG0780038403.01 | 0.828778 | 7.442411e-55 | 7.470361e-52 |
| MsG0780038403.01 | MsG0880044565.01 | 0.822813 | 1.937408e-53 | 1.639038e-50 |
| MsG0780038403.01 | MsG0880046074.01 | 0.814338 | 1.620694e-51 | 1.084566e-48 |
| MsG0780038403.01 | MsG0880047470.01 | 0.806174 | 9.350099e-50 | 5.041080e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0780038403.01 | MsG0180005007.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780038403.01.T01 | MTR_7g053520 | 94.037 | 218 | 10 | 2 | 1 | 216 | 1 | 217 | 1.71e-138 | 386 |
| MsG0780038403.01.T01 | MTR_2g064300 | 83.945 | 218 | 31 | 3 | 1 | 216 | 1 | 216 | 4.43e-118 | 335 |
| MsG0780038403.01.T01 | MTR_2g031760 | 41.212 | 165 | 92 | 3 | 53 | 216 | 30 | 190 | 1.79e-35 | 124 |
| MsG0780038403.01.T01 | MTR_8g076780 | 47.973 | 148 | 67 | 2 | 78 | 216 | 49 | 195 | 1.49e-31 | 114 |
| MsG0780038403.01.T01 | MTR_2g031960 | 48.120 | 133 | 64 | 2 | 85 | 216 | 27 | 155 | 1.29e-29 | 108 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780038403.01.T01 | AT1G70190 | 56.364 | 220 | 80 | 3 | 1 | 216 | 1 | 208 | 2.03e-69 | 211 |
| MsG0780038403.01.T01 | AT1G70190 | 56.364 | 220 | 80 | 3 | 1 | 216 | 1 | 208 | 2.03e-69 | 211 |
| MsG0780038403.01.T01 | AT4G36420 | 44.211 | 190 | 91 | 4 | 29 | 216 | 3 | 179 | 6.42e-34 | 120 |
| MsG0780038403.01.T01 | AT3G06040 | 44.667 | 150 | 74 | 3 | 68 | 216 | 45 | 186 | 4.00e-31 | 113 |
| MsG0780038403.01.T01 | AT3G06040 | 44.667 | 150 | 74 | 3 | 68 | 216 | 45 | 186 | 4.00e-31 | 113 |
| MsG0780038403.01.T01 | AT3G06040 | 44.667 | 150 | 74 | 3 | 68 | 216 | 45 | 186 | 4.00e-31 | 113 |
| MsG0780038403.01.T01 | AT4G37660 | 47.727 | 132 | 61 | 2 | 85 | 216 | 44 | 167 | 6.75e-26 | 99.0 |
| MsG0780038403.01.T01 | AT2G03130 | 55.294 | 85 | 38 | 0 | 132 | 216 | 7 | 91 | 6.13e-16 | 71.2 |
Find 49 sgRNAs with CRISPR-Local
Find 61 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCCTTGATGACCTTAATTT+TGG | 0.127344 | 7:+45951505 | None:intergenic |
| GAAACCAGGATCTGTAAATT+TGG | 0.208162 | 7:-45951624 | MsG0780038403.01.T01:CDS |
| ACACCTTTCTTTATAATAGA+AGG | 0.301571 | 7:+45951425 | None:intergenic |
| TGAAGCAGCTTCCAAAATTA+AGG | 0.341436 | 7:-45951516 | MsG0780038403.01.T01:CDS |
| TCTAGCTGAAGGTGCAGAAT+TGG | 0.346308 | 7:-45951702 | MsG0780038403.01.T01:CDS |
| TTGTCTATACAACAAACTAT+TGG | 0.364579 | 7:+45951949 | None:intergenic |
| CCAGCCAAATTTACAGATCC+TGG | 0.386608 | 7:+45951620 | None:intergenic |
| GAAGGAGGCAAAGGATTTAG+TGG | 0.417133 | 7:-45951456 | MsG0780038403.01.T01:CDS |
| TGTCTATACAACAAACTATT+GGG | 0.417171 | 7:+45951950 | None:intergenic |
| CTTTCCTTGCAGCTTGACCT+AGG | 0.449900 | 7:+45951873 | None:intergenic |
| CCAGGATCTGTAAATTTGGC+TGG | 0.453905 | 7:-45951620 | MsG0780038403.01.T01:CDS |
| CCTCTTACTGCTAGCACTGT+AGG | 0.457292 | 7:-45951926 | MsG0780038403.01.T01:CDS |
| CTAGCTGAAGGTGCAGAATT+GGG | 0.458679 | 7:-45951701 | MsG0780038403.01.T01:CDS |
| ATCTGTAAATTTGGCTGGAA+TGG | 0.461697 | 7:-45951615 | MsG0780038403.01.T01:CDS |
| CCTACAGTGCTAGCAGTAAG+AGG | 0.466523 | 7:+45951926 | None:intergenic |
| TCTAGGCGCAATTGTCGTTA+TGG | 0.475224 | 7:-45951360 | MsG0780038403.01.T01:CDS |
| TCAGTAGGATCAAATGTTGC+AGG | 0.479265 | 7:+45951785 | None:intergenic |
| AAAGGTGTTTCGAAGGAAGA+AGG | 0.479617 | 7:-45951410 | MsG0780038403.01.T01:CDS |
| GGTTTCATAAATCCCACATT+GGG | 0.482477 | 7:+45951641 | None:intergenic |
| AGCTGCAAGGAAAGAGGAGG+AGG | 0.499403 | 7:-45951864 | MsG0780038403.01.T01:CDS |
| TGCAAGGAAAGAGGAGGAGG+AGG | 0.500493 | 7:-45951861 | MsG0780038403.01.T01:CDS |
| ACACCTTCTATTATAAAGAA+AGG | 0.509780 | 7:-45951428 | MsG0780038403.01.T01:CDS |
| GTTGCAGGATCAAAATCAGT+TGG | 0.513514 | 7:+45951800 | None:intergenic |
| CTGAAAACTCTTTCCGATGG+AGG | 0.526100 | 7:+45951752 | None:intergenic |
| TTCCAAAATTAAGGTCATCA+AGG | 0.526956 | 7:-45951507 | MsG0780038403.01.T01:CDS |
| TTTCAGGCTTGTTGATGAAG+TGG | 0.533852 | 7:-45951735 | MsG0780038403.01.T01:CDS |
| TATAAAGAAAGGTGTTTCGA+AGG | 0.534435 | 7:-45951417 | MsG0780038403.01.T01:CDS |
| AAGGTCATCAAGGAGGTTCG+AGG | 0.535857 | 7:-45951497 | MsG0780038403.01.T01:CDS |
| CTCTTACTGCTAGCACTGTA+GGG | 0.537187 | 7:-45951925 | MsG0780038403.01.T01:CDS |
| TGATCATCGTGGGCCTCCAT+CGG | 0.539706 | 7:-45951765 | MsG0780038403.01.T01:CDS |
| ATAGAGAAATTGAAAGCTCT+AGG | 0.542277 | 7:-45951377 | MsG0780038403.01.T01:CDS |
| TTTAGGTTTGAAGGAGGCAA+AGG | 0.553743 | 7:-45951465 | MsG0780038403.01.T01:CDS |
| AGGTCAAGCTGCAAGGAAAG+AGG | 0.555154 | 7:-45951870 | MsG0780038403.01.T01:CDS |
| TTGCAGGATCAAAATCAGTT+GGG | 0.555773 | 7:+45951801 | None:intergenic |
| AGCCTGAAAACTCTTTCCGA+TGG | 0.583898 | 7:+45951749 | None:intergenic |
| ACAATAAATACAAGAAACCT+AGG | 0.583969 | 7:-45951890 | MsG0780038403.01.T01:CDS |
| TGGTTTCATAAATCCCACAT+TGG | 0.595026 | 7:+45951640 | None:intergenic |
| GGAGGCCCACGATGATCAGT+AGG | 0.597332 | 7:+45951770 | None:intergenic |
| TACTGATTTAGGTTTGAAGG+AGG | 0.609454 | 7:-45951471 | MsG0780038403.01.T01:CDS |
| GAAACCTAGGTCAAGCTGCA+AGG | 0.610947 | 7:-45951877 | MsG0780038403.01.T01:CDS |
| CAAAATTAAGGTCATCAAGG+AGG | 0.615684 | 7:-45951504 | MsG0780038403.01.T01:CDS |
| AACATCAGCAGAAGCCAAGG+AGG | 0.619521 | 7:-45951585 | MsG0780038403.01.T01:CDS |
| GCATCTCTAACTCTAGCTGA+AGG | 0.625143 | 7:-45951713 | MsG0780038403.01.T01:CDS |
| TCAAGCTGCAAGGAAAGAGG+AGG | 0.631410 | 7:-45951867 | MsG0780038403.01.T01:CDS |
| AATGTGGGATTTATGAAACC+AGG | 0.639785 | 7:-45951638 | MsG0780038403.01.T01:CDS |
| GATAAAGGAGATGCCCAATG+TGG | 0.647613 | 7:-45951654 | MsG0780038403.01.T01:CDS |
| ATAAAGGAGATGCCCAATGT+GGG | 0.651305 | 7:-45951653 | MsG0780038403.01.T01:CDS |
| TTGATCCTACTGATCATCGT+GGG | 0.654861 | 7:-45951775 | MsG0780038403.01.T01:CDS |
| TTTGATCCTACTGATCATCG+TGG | 0.702613 | 7:-45951776 | MsG0780038403.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTCAAATACAGTTTTTTC+TGG | + | Chr7:45951787-45951806 | None:intergenic | 20.0% |
| ! | ACAATAAATACAAGAAACCT+AGG | - | Chr7:45951448-45951467 | MsG0780038403.01.T01:CDS | 25.0% |
| ! | ACACCTTCTATTATAAAGAA+AGG | - | Chr7:45951910-45951929 | MsG0780038403.01.T01:CDS | 25.0% |
| ! | ACACCTTTCTTTATAATAGA+AGG | + | Chr7:45951916-45951935 | None:intergenic | 25.0% |
| ! | TGTCTATACAACAAACTATT+GGG | + | Chr7:45951391-45951410 | None:intergenic | 25.0% |
| ! | TTGTCTATACAACAAACTAT+TGG | + | Chr7:45951392-45951411 | None:intergenic | 25.0% |
| !!! | TGAGTTTGCTTTTAAGAATA+AGG | - | Chr7:45951356-45951375 | MsG0780038403.01.T01:CDS | 25.0% |
| !!! | TTTTACTGATTTAGGTTTGA+AGG | - | Chr7:45951864-45951883 | MsG0780038403.01.T01:CDS | 25.0% |
| ATAGAGAAATTGAAAGCTCT+AGG | - | Chr7:45951961-45951980 | MsG0780038403.01.T01:CDS | 30.0% | |
| GATGAAAAAAATGGGGATAA+AGG | - | Chr7:45951669-45951688 | MsG0780038403.01.T01:CDS | 30.0% | |
| TTCCAAAATTAAGGTCATCA+AGG | - | Chr7:45951831-45951850 | MsG0780038403.01.T01:CDS | 30.0% | |
| !! | TATAAAGAAAGGTGTTTCGA+AGG | - | Chr7:45951921-45951940 | MsG0780038403.01.T01:CDS | 30.0% |
| AATGTGGGATTTATGAAACC+AGG | - | Chr7:45951700-45951719 | MsG0780038403.01.T01:CDS | 35.0% | |
| ATCTGTAAATTTGGCTGGAA+TGG | - | Chr7:45951723-45951742 | MsG0780038403.01.T01:CDS | 35.0% | |
| CAAAATTAAGGTCATCAAGG+AGG | - | Chr7:45951834-45951853 | MsG0780038403.01.T01:CDS | 35.0% | |
| CTCGCTCTGATGAAAAAAAT+GGG | - | Chr7:45951661-45951680 | MsG0780038403.01.T01:CDS | 35.0% | |
| GAAACCAGGATCTGTAAATT+TGG | - | Chr7:45951714-45951733 | MsG0780038403.01.T01:CDS | 35.0% | |
| GGTTTCATAAATCCCACATT+GGG | + | Chr7:45951700-45951719 | None:intergenic | 35.0% | |
| TCGCTCTGATGAAAAAAATG+GGG | - | Chr7:45951662-45951681 | MsG0780038403.01.T01:CDS | 35.0% | |
| TCTCGCTCTGATGAAAAAAA+TGG | - | Chr7:45951660-45951679 | MsG0780038403.01.T01:CDS | 35.0% | |
| TGAAGCAGCTTCCAAAATTA+AGG | - | Chr7:45951822-45951841 | MsG0780038403.01.T01:CDS | 35.0% | |
| TGGTTTCATAAATCCCACAT+TGG | + | Chr7:45951701-45951720 | None:intergenic | 35.0% | |
| TTGCAGGATCAAAATCAGTT+GGG | + | Chr7:45951540-45951559 | None:intergenic | 35.0% | |
| !! | AATTTTGGAAGCTGCTTCAT+AGG | + | Chr7:45951821-45951840 | None:intergenic | 35.0% |
| !! | CTCCTTGATGACCTTAATTT+TGG | + | Chr7:45951836-45951855 | None:intergenic | 35.0% |
| !! | TACTGATTTAGGTTTGAAGG+AGG | - | Chr7:45951867-45951886 | MsG0780038403.01.T01:CDS | 35.0% |
| !!! | GTTCGAGGTTTTACTGATTT+AGG | - | Chr7:45951856-45951875 | MsG0780038403.01.T01:CDS | 35.0% |
| AAAAACATCAGCAGAAGCCA+AGG | - | Chr7:45951750-45951769 | MsG0780038403.01.T01:CDS | 40.0% | |
| ATAAAGGAGATGCCCAATGT+GGG | - | Chr7:45951685-45951704 | MsG0780038403.01.T01:CDS | 40.0% | |
| GTTGCAGGATCAAAATCAGT+TGG | + | Chr7:45951541-45951560 | None:intergenic | 40.0% | |
| TCAGTAGGATCAAATGTTGC+AGG | + | Chr7:45951556-45951575 | None:intergenic | 40.0% | |
| TTGATCCTACTGATCATCGT+GGG | - | Chr7:45951563-45951582 | MsG0780038403.01.T01:CDS | 40.0% | |
| TTTGATCCTACTGATCATCG+TGG | - | Chr7:45951562-45951581 | MsG0780038403.01.T01:CDS | 40.0% | |
| ! | TTTAGGTTTGAAGGAGGCAA+AGG | - | Chr7:45951873-45951892 | MsG0780038403.01.T01:CDS | 40.0% |
| !! | AAAGGTGTTTCGAAGGAAGA+AGG | - | Chr7:45951928-45951947 | MsG0780038403.01.T01:CDS | 40.0% |
| !! | TTTCAGGCTTGTTGATGAAG+TGG | - | Chr7:45951603-45951622 | MsG0780038403.01.T01:CDS | 40.0% |
| AGCCTGAAAACTCTTTCCGA+TGG | + | Chr7:45951592-45951611 | None:intergenic | 45.0% | |
| CCAGCCAAATTTACAGATCC+TGG | + | Chr7:45951721-45951740 | None:intergenic | 45.0% | |
| CCAGGATCTGTAAATTTGGC+TGG | - | Chr7:45951718-45951737 | MsG0780038403.01.T01:CDS | 45.0% | |
| CTCTTACTGCTAGCACTGTA+GGG | - | Chr7:45951413-45951432 | MsG0780038403.01.T01:CDS | 45.0% | |
| CTGAAAACTCTTTCCGATGG+AGG | + | Chr7:45951589-45951608 | None:intergenic | 45.0% | |
| GAAGGAGGCAAAGGATTTAG+TGG | - | Chr7:45951882-45951901 | MsG0780038403.01.T01:CDS | 45.0% | |
| GATAAAGGAGATGCCCAATG+TGG | - | Chr7:45951684-45951703 | MsG0780038403.01.T01:CDS | 45.0% | |
| GCATCTCTAACTCTAGCTGA+AGG | - | Chr7:45951625-45951644 | MsG0780038403.01.T01:CDS | 45.0% | |
| TCTAGGCGCAATTGTCGTTA+TGG | - | Chr7:45951978-45951997 | MsG0780038403.01.T01:CDS | 45.0% | |
| ! | CTAGCTGAAGGTGCAGAATT+GGG | - | Chr7:45951637-45951656 | MsG0780038403.01.T01:CDS | 45.0% |
| ! | CTCCATCGGAAAGAGTTTTC+AGG | - | Chr7:45951587-45951606 | MsG0780038403.01.T01:CDS | 45.0% |
| ! | TCTAGCTGAAGGTGCAGAAT+TGG | - | Chr7:45951636-45951655 | MsG0780038403.01.T01:CDS | 45.0% |
| AACATCAGCAGAAGCCAAGG+AGG | - | Chr7:45951753-45951772 | MsG0780038403.01.T01:CDS | 50.0% | |
| AAGGTCATCAAGGAGGTTCG+AGG | - | Chr7:45951841-45951860 | MsG0780038403.01.T01:CDS | 50.0% | |
| AGGTCAAGCTGCAAGGAAAG+AGG | - | Chr7:45951468-45951487 | MsG0780038403.01.T01:CDS | 50.0% | |
| CCTACAGTGCTAGCAGTAAG+AGG | + | Chr7:45951415-45951434 | None:intergenic | 50.0% | |
| CCTCTTACTGCTAGCACTGT+AGG | - | Chr7:45951412-45951431 | MsG0780038403.01.T01:CDS | 50.0% | |
| CTTTCCTTGCAGCTTGACCT+AGG | + | Chr7:45951468-45951487 | None:intergenic | 50.0% | |
| GAAACCTAGGTCAAGCTGCA+AGG | - | Chr7:45951461-45951480 | MsG0780038403.01.T01:CDS | 50.0% | |
| TCAAGCTGCAAGGAAAGAGG+AGG | - | Chr7:45951471-45951490 | MsG0780038403.01.T01:CDS | 50.0% | |
| !!! | TTCTGGCTTTTGCTCCTCCT+TGG | + | Chr7:45951770-45951789 | None:intergenic | 50.0% |
| AGCTGCAAGGAAAGAGGAGG+AGG | - | Chr7:45951474-45951493 | MsG0780038403.01.T01:CDS | 55.0% | |
| TGATCATCGTGGGCCTCCAT+CGG | - | Chr7:45951573-45951592 | MsG0780038403.01.T01:CDS | 55.0% | |
| TGCAAGGAAAGAGGAGGAGG+AGG | - | Chr7:45951477-45951496 | MsG0780038403.01.T01:CDS | 55.0% | |
| GGAGGCCCACGATGATCAGT+AGG | + | Chr7:45951571-45951590 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 45951355 | 45952005 | 45951355 | ID=MsG0780038403.01;Name=MsG0780038403.01 |
| Chr7 | mRNA | 45951355 | 45952005 | 45951355 | ID=MsG0780038403.01.T01;Parent=MsG0780038403.01;Name=MsG0780038403.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|216 |
| Chr7 | exon | 45951355 | 45952005 | 45951355 | ID=MsG0780038403.01.T01:exon:4275;Parent=MsG0780038403.01.T01 |
| Chr7 | CDS | 45951355 | 45952005 | 45951355 | ID=MsG0780038403.01.T01:cds;Parent=MsG0780038403.01.T01 |
| Gene Sequence |
| Protein sequence |
