AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580024197.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024197.01.T01 MTR_5g007240 100.000 117 0 0 1 117 50 166 3.47e-81 235
MsG0580024197.01.T01 MTR_5g096490 100.000 117 0 0 1 117 50 166 3.47e-81 235
MsG0580024197.01.T01 MTR_5g096490 100.000 117 0 0 1 117 17 133 3.82e-81 234
MsG0580024197.01.T01 MTR_4g051390 42.735 117 65 1 2 116 5 121 4.80e-31 107
MsG0580024197.01.T01 MTR_1779s0020 42.735 117 65 1 2 116 5 121 1.04e-29 103
MsG0580024197.01.T01 MTR_2g087550 41.026 117 67 1 2 116 5 121 6.95e-28 101
MsG0580024197.01.T01 MTR_0002s0780 41.739 115 64 1 5 116 135 249 7.70e-25 94.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024197.01.T01 AT5G46160 78.226 124 20 1 1 117 49 172 4.99e-62 187
MsG0580024197.01.T01 AT5G46160 78.226 124 20 1 1 117 50 173 5.69e-62 187
MsG0580024197.01.T01 AT1G17560 59.310 145 30 3 1 116 51 195 1.11e-48 154
MsG0580024197.01.T01 AT1G17560 59.310 145 30 3 1 116 28 172 1.65e-48 153
MsG0580024197.01.T01 ATCG00780 44.444 117 63 1 2 116 5 121 1.43e-32 110

Find 33 sgRNAs with CRISPR-Local

Find 34 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGAAGGTTGTGGACAATTC+TGG 0.240087 5:-1842215 MsG0580024197.01.T01:CDS
GGACAATTCTGGAGCTAAAA+AGG 0.321429 5:-1842204 MsG0580024197.01.T01:CDS
GACAAACAAGGGCAGCCTAT+TGG 0.362318 5:-1841981 MsG0580024197.01.T01:CDS
ACAAACAAGGGCAGCCTATT+GGG 0.366691 5:-1841980 MsG0580024197.01.T01:CDS
GTCAAGATCCTTACCTTAGC+TGG 0.374088 5:-1841909 MsG0580024197.01.T01:CDS
GTGAAGGAAGCACATCCTAA+TGG 0.397241 5:-1842116 MsG0580024197.01.T01:CDS
TGCCTCAACTCATGAGGAAC+TGG 0.420523 5:+1841942 None:intergenic
CCGAAAACTCTGGTCCCAAT+AGG 0.444490 5:+1841966 None:intergenic
CGCGCGTTGGCAGGGAAGAA+AGG 0.457182 5:-1842170 MsG0580024197.01.T01:CDS
GGGAAGAAAGGAGCAAGATT+AGG 0.462937 5:-1842158 MsG0580024197.01.T01:CDS
ATGTGCATACGCGCGTTGGC+AGG 0.463329 5:-1842179 MsG0580024197.01.T01:CDS
TCCTAATGGAAAAGTGAAGA+AGG 0.493277 5:-1842102 MsG0580024197.01.T01:CDS
CTGCCATCACACCTACCCTT+TGG 0.494271 5:+1842041 None:intergenic
ATGCCAAAGGGTAGGTGTGA+TGG 0.533908 5:-1842044 MsG0580024197.01.T01:CDS
CAATATGCCCAGCTAAGGTA+AGG 0.539065 5:+1841901 None:intergenic
CATTGCTGCACGAACAACTA+CGG 0.550383 5:+1842064 None:intergenic
AGGAACTGGTCCGAAAACTC+TGG 0.550755 5:+1841956 None:intergenic
TCAAGATCCTTACCTTAGCT+GGG 0.562147 5:-1841908 MsG0580024197.01.T01:CDS
CTATGCAATATGCCCAGCTA+AGG 0.565511 5:+1841896 None:intergenic
TGTTCGTGCAGCAATGCCAA+AGG 0.568662 5:-1842057 MsG0580024197.01.T01:CDS
TGTGCATACGCGCGTTGGCA+GGG 0.580036 5:-1842178 MsG0580024197.01.T01:CDS
GACCAGTTCCTCATGAGTTG+AGG 0.580661 5:-1841944 MsG0580024197.01.T01:CDS
ACAGATGAGGACTGTTCTGA+AGG 0.580846 5:-1842231 None:intergenic
GAGGACTGTTCTGAAGGTTG+TGG 0.589490 5:-1842225 MsG0580024197.01.T01:CDS
GGTGATGTGCATACGCGCGT+TGG 0.592616 5:-1842183 MsG0580024197.01.T01:CDS
GTTCGTGCAGCAATGCCAAA+GGG 0.599925 5:-1842056 MsG0580024197.01.T01:CDS
CCTAATGGAAAAGTGAAGAA+GGG 0.626002 5:-1842101 MsG0580024197.01.T01:CDS
TTCTTCTGCCTCAACTCATG+AGG 0.632900 5:+1841936 None:intergenic
GTGCAGCAATGCCAAAGGGT+AGG 0.641762 5:-1842052 MsG0580024197.01.T01:CDS
GTTGTGCTTGTTGACAAACA+AGG 0.656177 5:-1841993 MsG0580024197.01.T01:CDS
TATGATACAAGCATCAGTGA+AGG 0.662607 5:-1842132 MsG0580024197.01.T01:CDS
TTGTGCTTGTTGACAAACAA+GGG 0.669343 5:-1841992 MsG0580024197.01.T01:CDS
GGGTAGGTGTGATGGCAGTG+AGG 0.701393 5:-1842036 MsG0580024197.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
CCTAATGGAAAAGTGAAGAA+GGG - Chr5:1842022-1842041 MsG0580024197.01.T01:CDS 35.0%
TATGATACAAGCATCAGTGA+AGG - Chr5:1841991-1842010 MsG0580024197.01.T01:CDS 35.0%
TCCTAATGGAAAAGTGAAGA+AGG - Chr5:1842021-1842040 MsG0580024197.01.T01:CDS 35.0%
! TTGTGCTTGTTGACAAACAA+GGG - Chr5:1842131-1842150 MsG0580024197.01.T01:CDS 35.0%
TCAAGATCCTTACCTTAGCT+GGG - Chr5:1842215-1842234 MsG0580024197.01.T01:CDS 40.0%
! GTTGTGCTTGTTGACAAACA+AGG - Chr5:1842130-1842149 MsG0580024197.01.T01:CDS 40.0%
!! CCCTTCTTCACTTTTCCATT+AGG + Chr5:1842025-1842044 None:intergenic 40.0%
!! GGACAATTCTGGAGCTAAAA+AGG - Chr5:1841919-1841938 MsG0580024197.01.T01:CDS 40.0%
ACAAACAAGGGCAGCCTATT+GGG - Chr5:1842143-1842162 MsG0580024197.01.T01:CDS 45.0%
CAATATGCCCAGCTAAGGTA+AGG + Chr5:1842225-1842244 None:intergenic 45.0%
CATTGCTGCACGAACAACTA+CGG + Chr5:1842062-1842081 None:intergenic 45.0%
CTATGCAATATGCCCAGCTA+AGG + Chr5:1842230-1842249 None:intergenic 45.0%
CTGAAGGTTGTGGACAATTC+TGG - Chr5:1841908-1841927 MsG0580024197.01.T01:CDS 45.0%
GGGAAGAAAGGAGCAAGATT+AGG - Chr5:1841965-1841984 MsG0580024197.01.T01:CDS 45.0%
GTCAAGATCCTTACCTTAGC+TGG - Chr5:1842214-1842233 MsG0580024197.01.T01:CDS 45.0%
TTCTTCTGCCTCAACTCATG+AGG + Chr5:1842190-1842209 None:intergenic 45.0%
! CCTATTGGGACCAGAGTTTT+CGG - Chr5:1842157-1842176 MsG0580024197.01.T01:CDS 45.0%
! GTGAAGGAAGCACATCCTAA+TGG - Chr5:1842007-1842026 MsG0580024197.01.T01:CDS 45.0%
AGGAACTGGTCCGAAAACTC+TGG + Chr5:1842170-1842189 None:intergenic 50.0%
ATGCCAAAGGGTAGGTGTGA+TGG - Chr5:1842079-1842098 MsG0580024197.01.T01:CDS 50.0%
CCGAAAACTCTGGTCCCAAT+AGG + Chr5:1842160-1842179 None:intergenic 50.0%
GACAAACAAGGGCAGCCTAT+TGG - Chr5:1842142-1842161 MsG0580024197.01.T01:CDS 50.0%
GACCAGTTCCTCATGAGTTG+AGG - Chr5:1842179-1842198 MsG0580024197.01.T01:CDS 50.0%
GTTCGTGCAGCAATGCCAAA+GGG - Chr5:1842067-1842086 MsG0580024197.01.T01:CDS 50.0%
TGCCTCAACTCATGAGGAAC+TGG + Chr5:1842184-1842203 None:intergenic 50.0%
TGTTCGTGCAGCAATGCCAA+AGG - Chr5:1842066-1842085 MsG0580024197.01.T01:CDS 50.0%
!! GAGGACTGTTCTGAAGGTTG+TGG - Chr5:1841898-1841917 MsG0580024197.01.T01:CDS 50.0%
CTGCCATCACACCTACCCTT+TGG + Chr5:1842085-1842104 None:intergenic 55.0%
GTGCAGCAATGCCAAAGGGT+AGG - Chr5:1842071-1842090 MsG0580024197.01.T01:CDS 55.0%
GGGTAGGTGTGATGGCAGTG+AGG - Chr5:1842087-1842106 MsG0580024197.01.T01:CDS 60.0%
GGTGATGTGCATACGCGCGT+TGG - Chr5:1841940-1841959 MsG0580024197.01.T01:CDS 60.0%
! ATGTGCATACGCGCGTTGGC+AGG - Chr5:1841944-1841963 MsG0580024197.01.T01:CDS 60.0%
! TGTGCATACGCGCGTTGGCA+GGG - Chr5:1841945-1841964 MsG0580024197.01.T01:CDS 60.0%
! CGCGCGTTGGCAGGGAAGAA+AGG - Chr5:1841953-1841972 MsG0580024197.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr5 gene 1841896 1842249 1841896 ID=MsG0580024197.01;Name=MsG0580024197.01
Chr5 mRNA 1841896 1842249 1841896 ID=MsG0580024197.01.T01;Parent=MsG0580024197.01;Name=MsG0580024197.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|117
Chr5 exon 1841896 1842249 1841896 ID=MsG0580024197.01.T01:exon:1031;Parent=MsG0580024197.01.T01
Chr5 CDS 1841896 1842249 1841896 ID=MsG0580024197.01.T01:cds;Parent=MsG0580024197.01.T01
Gene Sequence

>MsG0580024197.01.T01

ATGAGGACTGTTCTGAAGGTTGTGGACAATTCTGGAGCTAAAAAGGTGATGTGCATACGCGCGTTGGCAGGGAAGAAAGGAGCAAGATTAGGTGATATGATACAAGCATCAGTGAAGGAAGCACATCCTAATGGAAAAGTGAAGAAGGGAGCTGTTGTATTTGCCGTAGTTGTTCGTGCAGCAATGCCAAAGGGTAGGTGTGATGGCAGTGAGGTCAAGTTTGACGACAATGCTGTTGTGCTTGTTGACAAACAAGGGCAGCCTATTGGGACCAGAGTTTTCGGACCAGTTCCTCATGAGTTGAGGCAGAAGAAGCATGTCAAGATCCTTACCTTAGCTGGGCATATTGCATAG

Protein sequence

>MsG0580024197.01.T01

MRTVLKVVDNSGAKKVMCIRALAGKKGARLGDMIQASVKEAHPNGKVKKGAVVFAVVVRAAMPKGRCDGSEVKFDDNAVVLVDKQGQPIGTRVFGPVPHELRQKKHVKILTLAGHIA*