AlfalfaGEDB | MsG0480021006.01

M. sativa cultivar ZhongmuNo.1 / MsG0480021006.01

KEGG: K17777
GO: -
PFAM: PF02953

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021006.01.T01	XP_013456128.1	100	93	0	0	1	93	1	93	2.15E-62	194

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021006.01.T01	Q9XGX8	79.57	93	19	0	1	93	1	93	8.06E-53	162

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021006.01.T01	I3SBU8	100.000	93	0	0	1	93	1	93	1.03e-62	194

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048197.01	MsG0480021006.01	0.820170	7.902568e-53	6.206894e-50
MsG0080048687.01	MsG0480021006.01	0.867681	1.143687e-65	4.027234e-62
MsG0080048784.01	MsG0480021006.01	0.804220	2.398624e-49	1.229769e-46
MsG0080048843.01	MsG0480021006.01	0.802342	5.871904e-49	2.869395e-46
MsG0080048968.01	MsG0480021006.01	0.843490	1.357418e-58	2.127203e-55
MsG0080048971.01	MsG0480021006.01	0.888053	8.300680e-73	6.328963e-69
MsG0180000055.01	MsG0480021006.01	0.823993	1.026735e-53	8.979783e-51
MsG0180000139.01	MsG0480021006.01	0.869407	3.169635e-66	1.188301e-62
MsG0180000331.01	MsG0480021006.01	0.828100	1.084402e-54	1.067327e-51
MsG0180000339.01	MsG0480021006.01	0.906764	1.047249e-80	1.799543e-76
MsG0180000575.01	MsG0480021006.01	0.834817	2.406816e-56	2.888653e-53
MsG0180000611.01	MsG0480021006.01	0.812520	4.069113e-51	2.592832e-48
MsG0180000614.01	MsG0480021006.01	0.848740	5.054286e-60	9.367568e-57
MsG0180000615.01	MsG0480021006.01	0.831996	1.216111e-55	1.341758e-52
MsG0180000835.01	MsG0480021006.01	0.822447	2.357962e-53	1.974156e-50
MsG0180002023.01	MsG0480021006.01	0.816049	6.757745e-52	4.737935e-49
MsG0180002458.01	MsG0480021006.01	0.805858	1.089723e-49	5.828056e-47
MsG0180002547.01	MsG0480021006.01	0.836382	9.666999e-57	1.216249e-53
MsG0180003115.01	MsG0480021006.01	0.823248	1.534506e-53	1.314116e-50
MsG0180003117.01	MsG0480021006.01	0.875723	2.460902e-68	1.161435e-64
MsG0180003612.01	MsG0480021006.01	0.814100	1.829500e-51	1.216353e-48
MsG0180003651.01	MsG0480021006.01	0.841318	5.110998e-58	7.481117e-55
MsG0180003793.01	MsG0480021006.01	0.908033	2.658395e-81	4.841151e-77
MsG0180004009.01	MsG0480021006.01	0.882187	1.285290e-70	7.752970e-67
MsG0180004128.01	MsG0480021006.01	0.821337	4.260194e-53	3.456260e-50
MsG0180004129.01	MsG0480021006.01	0.916368	1.937128e-85	5.309276e-81
MsG0180004130.01	MsG0480021006.01	0.929703	4.745801e-93	2.690174e-88
MsG0180004152.01	MsG0480021006.01	0.820235	7.636101e-53	6.007966e-50
MsG0180004481.01	MsG0480021006.01	0.867816	1.035295e-65	3.662726e-62
MsG0180004482.01	MsG0480021006.01	0.864191	1.451779e-64	4.518756e-61
MsG0180004509.01	MsG0480021006.01	0.894295	2.820916e-75	2.787367e-71
MsG0180004767.01	MsG0480021006.01	0.809376	1.951111e-50	1.143696e-47
MsG0180004854.01	MsG0480021006.01	0.854928	8.876647e-62	2.014827e-58
MsG0180004861.01	MsG0480021006.01	0.820746	5.827210e-53	4.650251e-50
MsG0180004991.01	MsG0480021006.01	0.810604	1.061368e-50	6.426211e-48
MsG0180005382.01	MsG0480021006.01	0.819872	9.245955e-53	7.201745e-50
MsG0180005426.01	MsG0480021006.01	0.877620	5.431472e-69	2.748842e-65
MsG0180005436.01	MsG0480021006.01	0.811555	6.603643e-51	4.100328e-48
MsG0180005659.01	MsG0480021006.01	0.866209	3.370327e-65	1.126703e-61
MsG0180005851.01	MsG0480021006.01	0.856404	3.291724e-62	7.848036e-59
MsG0180005957.01	MsG0480021006.01	0.829611	4.671402e-55	4.805412e-52
MsG0180006013.01	MsG0480021006.01	0.842135	3.110120e-58	4.670872e-55
MsG0280006406.01	MsG0480021006.01	0.862937	3.556452e-64	1.059836e-60
MsG0280006424.01	MsG0480021006.01	0.844729	6.312593e-59	1.028521e-55
MsG0280010776.01	MsG0480021006.01	0.883622	3.840376e-71	2.453661e-67
MsG0280010801.01	MsG0480021006.01	0.874267	7.718252e-68	3.452685e-64
MsG0280011387.01	MsG0480021006.01	0.878524	2.619556e-69	1.372310e-65
MsG0380011614.01	MsG0480021006.01	0.861785	8.039962e-64	2.301229e-60
MsG0380011704.01	MsG0480021006.01	0.824539	7.642450e-54	6.789223e-51
MsG0380012073.01	MsG0480021006.01	0.877140	7.979000e-69	3.969458e-65
MsG0380012108.01	MsG0480021006.01	0.856411	3.277148e-62	7.815146e-59
MsG0380014175.01	MsG0480021006.01	0.820413	6.950810e-53	5.496372e-50
MsG0480018477.01	MsG0480021006.01	0.892348	1.724925e-74	1.570656e-70
MsG0480018894.01	MsG0480021006.01	0.811499	6.789312e-51	4.209615e-48
MsG0480018906.01	MsG0480021006.01	0.813479	2.506658e-51	1.638846e-48
MsG0480019380.01	MsG0480021006.01	0.822754	2.000037e-53	1.689245e-50
MsG0480020959.01	MsG0480021006.01	0.980676	8.996299e-151	2.184261e-144
MsG0480021006.01	MsG0480021037.01	0.806184	9.302694e-50	5.016875e-47
MsG0480021006.01	MsG0480021832.01	0.823843	1.113278e-53	9.696670e-51
MsG0480021006.01	MsG0480021904.01	0.845141	4.887257e-59	8.066454e-56
MsG0480021006.01	MsG0480021994.01	0.822080	2.868056e-53	2.376026e-50
MsG0480021006.01	MsG0480022035.01	0.853101	2.985835e-61	6.378330e-58
MsG0480021006.01	MsG0480022223.01	0.818367	2.033900e-52	1.519578e-49
MsG0480021006.01	MsG0480022252.01	0.815577	8.609105e-52	5.958257e-49
MsG0480021006.01	MsG0480022493.01	0.815026	1.141481e-51	7.781822e-49
MsG0480021006.01	MsG0480022711.01	0.813798	2.132846e-51	1.406490e-48
MsG0480021006.01	MsG0480022791.01	0.823808	1.134893e-53	9.875260e-51
MsG0480021006.01	MsG0480022913.01	0.821091	4.853434e-53	3.910841e-50
MsG0480021006.01	MsG0480022960.01	0.844614	6.777661e-59	1.100277e-55
MsG0480021006.01	MsG0480023055.01	0.801438	9.003417e-49	4.299734e-46
MsG0480021006.01	MsG0480023149.01	0.894435	2.473654e-75	2.459068e-71
MsG0480021006.01	MsG0480023221.01	0.827115	1.869555e-54	1.787879e-51
MsG0480021006.01	MsG0480023467.01	0.834126	3.589992e-56	4.220375e-53
MsG0480021006.01	MsG0480023559.01	0.847146	1.390903e-59	2.449465e-56
MsG0480021006.01	MsG0480023968.01	0.822292	2.561592e-53	2.135191e-50
MsG0480021006.01	MsG0580024084.01	0.828434	9.009870e-55	8.954699e-52
MsG0480021006.01	MsG0580024369.01	0.802280	6.046247e-49	2.949902e-46
MsG0480021006.01	MsG0580024390.01	0.822137	2.782377e-53	2.308925e-50
MsG0480021006.01	MsG0580024544.01	0.833419	5.391566e-56	6.205742e-53
MsG0480021006.01	MsG0580024754.01	0.817493	3.204640e-52	2.337378e-49
MsG0480021006.01	MsG0580025400.01	0.875852	2.221332e-68	1.053606e-64
MsG0480021006.01	MsG0580025421.01	0.842772	2.107662e-58	3.229030e-55
MsG0480021006.01	MsG0580025651.01	0.808220	3.447399e-50	1.959905e-47
MsG0480021006.01	MsG0580026226.01	0.814717	1.336282e-51	9.034229e-49
MsG0480021006.01	MsG0580027043.01	0.879453	1.230472e-69	6.675303e-66
MsG0480021006.01	MsG0580027361.01	0.815346	9.691807e-52	6.665140e-49
MsG0480021006.01	MsG0580028597.01	0.805172	1.517273e-49	7.972701e-47
MsG0480021006.01	MsG0580029132.01	0.883893	3.050460e-71	1.970025e-67
MsG0480021006.01	MsG0580029283.01	0.862635	4.408604e-64	1.299833e-60
MsG0480021006.01	MsG0580029300.01	0.828851	7.144309e-55	7.186237e-52
MsG0480021006.01	MsG0580029783.01	0.800656	1.300851e-48	6.090823e-46
MsG0480021006.01	MsG0680030414.01	0.802653	5.065768e-49	2.495007e-46
MsG0480021006.01	MsG0680030721.01	0.805480	1.307826e-49	6.926638e-47
MsG0480021006.01	MsG0680031628.01	0.843172	1.650066e-58	2.559810e-55
MsG0480021006.01	MsG0680031820.01	0.817701	2.876943e-52	2.110287e-49
MsG0480021006.01	MsG0680032430.01	0.834108	3.626697e-56	4.261425e-53
MsG0480021006.01	MsG0680032770.01	0.846316	2.345099e-59	4.020150e-56
MsG0480021006.01	MsG0680034671.01	0.843331	1.496518e-58	2.333817e-55
MsG0480021006.01	MsG0680035306.01	0.846634	1.919657e-59	3.325211e-56
MsG0480021006.01	MsG0680035577.01	0.818187	2.234093e-52	1.660772e-49
MsG0480021006.01	MsG0680035848.01	0.833551	4.997129e-56	5.774462e-53
MsG0480021006.01	MsG0680035854.01	0.862552	4.676205e-64	1.374803e-60
MsG0480021006.01	MsG0680035888.01	0.812545	4.017843e-51	2.561921e-48
MsG0480021006.01	MsG0780036240.01	0.931858	2.025101e-94	1.314165e-89
MsG0480021006.01	MsG0780036547.01	0.833000	6.854591e-56	7.791812e-53
MsG0480021006.01	MsG0780036813.01	0.811163	8.032740e-51	4.936248e-48
MsG0480021006.01	MsG0780036822.01	0.812815	3.505445e-51	2.250887e-48
MsG0480021006.01	MsG0780037240.01	0.838945	2.127258e-57	2.893442e-54
MsG0480021006.01	MsG0780038400.01	0.904027	1.884171e-79	2.860327e-75
MsG0480021006.01	MsG0780038403.01	0.812151	4.897025e-51	3.089699e-48
MsG0480021006.01	MsG0780038587.01	0.887082	1.949695e-72	1.429187e-68
MsG0480021006.01	MsG0780039258.01	0.801708	7.925899e-49	3.810941e-46
MsG0480021006.01	MsG0780039340.01	0.827460	1.545591e-54	1.493162e-51
MsG0480021006.01	MsG0780039643.01	0.835715	1.427958e-56	1.760786e-53
MsG0480021006.01	MsG0780039848.01	0.843636	1.240699e-58	1.953501e-55
MsG0480021006.01	MsG0780039943.01	0.811575	6.537378e-51	4.061297e-48
MsG0480021006.01	MsG0780040070.01	0.812380	4.365630e-51	2.771441e-48
MsG0480021006.01	MsG0780040338.01	0.859679	3.501225e-63	9.323336e-60
MsG0480021006.01	MsG0780040569.01	0.801387	9.222441e-49	4.398635e-46
MsG0480021006.01	MsG0780040718.01	0.861963	7.087646e-64	2.041370e-60
MsG0480021006.01	MsG0780040861.01	0.809497	1.837945e-50	1.080779e-47
MsG0480021006.01	MsG0780040900.01	0.803286	3.747333e-49	1.875733e-46
MsG0480021006.01	MsG0780041137.01	0.823476	1.357377e-53	1.169957e-50
MsG0480021006.01	MsG0780041223.01	0.896046	5.371869e-76	5.712679e-72
MsG0480021006.01	MsG0780041224.01	0.806819	6.834424e-50	3.746080e-47
MsG0480021006.01	MsG0780041295.01	0.813389	2.624434e-51	1.711633e-48
MsG0480021006.01	MsG0780041462.01	0.852933	3.333647e-61	7.080256e-58
MsG0480021006.01	MsG0780041519.01	0.871094	8.875215e-67	3.535543e-63
MsG0480021006.01	MsG0780041688.01	0.817171	3.787448e-52	2.738109e-49
MsG0480021006.01	MsG0880041967.01	0.826095	3.273426e-54	3.039941e-51
MsG0480021006.01	MsG0880042128.01	0.855855	4.767133e-62	1.115953e-58
MsG0480021006.01	MsG0880042129.01	0.830351	3.083775e-55	3.240951e-52
MsG0480021006.01	MsG0880042519.01	0.867156	1.684151e-65	5.820319e-62
MsG0480021006.01	MsG0880042581.01	0.846501	2.087047e-59	3.599964e-56
MsG0480021006.01	MsG0880042991.01	0.836288	1.021895e-56	1.282180e-53
MsG0480021006.01	MsG0880043592.01	0.831562	1.555849e-55	1.694704e-52
MsG0480021006.01	MsG0880043616.01	0.801904	7.226480e-49	3.492068e-46
MsG0480021006.01	MsG0880043770.01	0.817884	2.616449e-52	1.928843e-49
MsG0480021006.01	MsG0880044711.01	0.856422	3.252730e-62	7.759938e-59
MsG0480021006.01	MsG0880045145.01	0.815306	9.889320e-52	6.793466e-49
MsG0480021006.01	MsG0880045656.01	0.842257	2.888035e-58	4.354056e-55
MsG0480021006.01	MsG0880045684.01	0.826458	2.683239e-54	2.517847e-51
MsG0480021006.01	MsG0880046074.01	0.828802	7.342538e-55	7.375459e-52
MsG0480021006.01	MsG0880046122.01	0.816663	4.923723e-52	3.510484e-49
MsG0480021006.01	MsG0880046177.01	0.876864	9.945849e-69	4.898545e-65
MsG0480021006.01	MsG0880046253.01	0.820317	7.312270e-53	5.766308e-50
MsG0480021006.01	MsG0880046321.01	0.811960	5.389133e-51	3.382410e-48
MsG0480021006.01	MsG0880046328.01	0.827049	1.938417e-54	1.850186e-51
MsG0480021006.01	MsG0880046444.01	0.839830	1.253287e-57	1.751752e-54
MsG0480021006.01	MsG0880046575.01	0.908269	2.056106e-81	3.787556e-77
MsG0480021006.01	MsG0880046631.01	0.821807	3.317145e-53	2.727052e-50
MsG0480021006.01	MsG0880046879.01	0.825547	4.416085e-54	4.037609e-51
MsG0480021006.01	MsG0880046933.01	0.862597	4.528309e-64	1.333473e-60
MsG0480021006.01	MsG0880047038.01	0.820986	5.131070e-53	4.122731e-50
MsG0480021006.01	MsG0880047212.01	0.807883	4.065902e-50	2.290964e-47
MsG0480021006.01	MsG0880047427.01	0.860510	1.964288e-63	5.382589e-60
MsG0480021006.01	MsG0880047428.01	0.873336	1.590987e-67	6.878988e-64
MsG0480021006.01	MsG0880047448.01	0.809378	1.948628e-50	1.142318e-47
MsG0480021006.01	MsG0880047470.01	0.800091	1.695318e-48	7.824721e-46
MsG0480021006.01	MsG0880047607.01	0.826630	2.440709e-54	2.301489e-51
MsG0380015027.01	MsG0480021006.01	0.864841	9.093608e-65	2.895536e-61
MsG0380015435.01	MsG0480021006.01	0.805370	1.379699e-49	7.286483e-47
MsG0380015818.01	MsG0480021006.01	0.844822	5.957782e-59	9.735790e-56
MsG0380016067.01	MsG0480021006.01	0.819834	9.429172e-53	7.336939e-50
MsG0380016290.01	MsG0480021006.01	0.851811	6.959328e-61	1.425391e-57
MsG0380016559.01	MsG0480021006.01	0.805187	1.506731e-49	7.920117e-47
MsG0380016560.01	MsG0480021006.01	0.812264	4.627495e-51	2.928561e-48
MsG0380017382.01	MsG0480021006.01	0.846987	1.537366e-59	2.693183e-56
MsG0380017556.01	MsG0480021006.01	0.809939	1.476714e-50	8.785880e-48
MsG0380017857.01	MsG0480021006.01	0.818030	2.424706e-52	1.794600e-49
MsG0480018110.01	MsG0480021006.01	0.833361	5.573916e-56	6.404515e-53
MsG0480018124.01	MsG0480021006.01	0.811447	6.971077e-51	4.316253e-48
MsG0480018335.01	MsG0480021006.01	0.807931	3.970978e-50	2.240295e-47
MsG0280006741.01	MsG0480021006.01	0.830256	3.252558e-55	3.409030e-52
MsG0280006759.01	MsG0480021006.01	0.824219	9.089304e-54	8.000496e-51
MsG0280006819.01	MsG0480021006.01	0.816504	5.343763e-52	3.793214e-49
MsG0280006836.01	MsG0480021006.01	0.820250	7.574469e-53	5.962139e-50
MsG0280006852.01	MsG0480021006.01	0.802967	4.364154e-49	2.166694e-46
MsG0280006993.01	MsG0480021006.01	0.892401	1.642725e-74	1.499382e-70
MsG0280007161.01	MsG0480021006.01	0.813434	2.564794e-51	1.674763e-48
MsG0280007222.01	MsG0480021006.01	0.804794	1.820265e-49	9.472423e-47
MsG0280007440.01	MsG0480021006.01	0.814858	1.243439e-51	8.438262e-49
MsG0280007441.01	MsG0480021006.01	0.813489	2.493954e-51	1.630999e-48
MsG0280007527.01	MsG0480021006.01	0.808500	3.003546e-50	1.720196e-47
MsG0280007634.01	MsG0480021006.01	0.802997	4.301821e-49	2.137325e-46
MsG0280007847.01	MsG0480021006.01	0.851160	1.063134e-60	2.132553e-57
MsG0280008118.01	MsG0480021006.01	0.897316	1.582455e-76	1.777764e-72
MsG0280008239.01	MsG0480021006.01	0.836655	8.237527e-57	1.044783e-53
MsG0280008283.01	MsG0480021006.01	0.891471	3.852697e-74	3.382628e-70
MsG0280009141.01	MsG0480021006.01	0.847101	1.430952e-59	2.516401e-56
MsG0280009230.01	MsG0480021006.01	0.882940	6.832232e-71	4.244717e-67
MsG0280009372.01	MsG0480021006.01	0.801228	9.938799e-49	4.721378e-46
MsG0280009375.01	MsG0480021006.01	0.915306	6.881043e-85	1.786080e-80
MsG0280009575.01	MsG0480021006.01	0.862946	3.534874e-64	1.053651e-60
MsG0280009716.01	MsG0480021006.01	0.817502	3.189553e-52	2.326958e-49
MsG0280010013.01	MsG0480021006.01	0.826184	3.118751e-54	2.903770e-51
MsG0280010258.01	MsG0480021006.01	0.813999	1.925579e-51	1.276735e-48
MsG0280010383.01	MsG0480021006.01	0.839173	1.855893e-57	2.542033e-54
MsG0280010495.01	MsG0480021006.01	0.853781	1.904785e-61	4.162623e-58
MsG0280010525.01	MsG0480021006.01	0.811452	6.953807e-51	4.306015e-48

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021006.01.T01	MTR_4g063925	100.000	93	0	0	1	93	1	93	2.61e-66	194
MsG0480021006.01.T01	MTR_4g021715	46.237	93	33	1	1	93	1	76	9.93e-25	89.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021006.01.T01	AT3G46560	78.495	93	20	0	1	93	1	93	4.57e-51	155

Find 32 sgRNAs with CRISPR-Local

Find 270 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACGATTATACGCAGTGATTT+AGG	0.125954	4:-51986414	None:intergenic
CAAAAGCATGATTGCTGATA+TGG	0.238381	4:+51986452	MsG0480021006.01.T01:CDS
CGATTATACGCAGTGATTTA+GGG	0.278105	4:-51986413	None:intergenic
TGAGCTAGGGTTTATCTTTA+CGG	0.322138	4:+51986366	MsG0480021006.01.T01:five_prime_UTR
TATAATCGTTCGATCCAAAA+TGG	0.326606	4:+51986428	MsG0480021006.01.T01:exon
AACCTCACCTGTCACGGATT+TGG	0.342119	4:-51986527	None:intergenic
TGCTGAAATCTAAATAAGTT+CGG	0.376638	4:+51990232	MsG0480021006.01.T01:three_prime_UTR
CCAGGTTATTATACATTCTC+AGG	0.398941	4:-51989926	None:intergenic
AATCTTGAGTTGCAGCTCCT+TGG	0.400935	4:-51990088	None:intergenic
ATTCTTTGTTTATCTGCCTC+TGG	0.426356	4:-51986483	None:intergenic
TCTTCTTCCACACTTGAGCT+AGG	0.452406	4:+51986352	MsG0480021006.01.T01:five_prime_UTR
GCTGAAATCTAAATAAGTTC+GGG	0.457487	4:+51990233	MsG0480021006.01.T01:three_prime_UTR
CTTCTTCCACACTTGAGCTA+GGG	0.471850	4:+51986353	MsG0480021006.01.T01:five_prime_UTR
TGAAGTCTATTAATTAACAT+TGG	0.487889	4:+51990110	MsG0480021006.01.T01:exon
AGAGGCAGATAAACAAAGAA+TGG	0.502607	4:+51986485	MsG0480021006.01.T01:CDS
TACAGCTTTGTTTGAAAGTG+AGG	0.506466	4:+51990161	MsG0480021006.01.T01:three_prime_UTR
ATCAGCTCCAAATCCGTGAC+AGG	0.514968	4:+51986520	MsG0480021006.01.T01:CDS
CTCTGCACATCTTCGAACAC+AGG	0.526331	4:-51990015	None:intergenic
TTTGGGAACCTCACCTGTCA+CGG	0.540460	4:-51986533	None:intergenic
CTGAAGCACTCTATGCGTGT+TGG	0.545186	4:+51990044	MsG0480021006.01.T01:CDS
ATAATGACAATCAAGAATCT+CGG	0.547637	4:-51990286	None:intergenic
GATTTGGAGCTGATCGATCA+TGG	0.557131	4:-51986511	None:intergenic
CCTGAGAATGTATAATAACC+TGG	0.571370	4:+51989926	MsG0480021006.01.T01:CDS
CATGAAAGCATCTCTCCACC+AGG	0.576768	4:-51989944	None:intergenic
ACTCTATGCGTGTTGGCATG+AGG	0.597417	4:+51990051	MsG0480021006.01.T01:CDS
GATAAACCCTAGCTCAAGTG+TGG	0.600396	4:-51986359	None:intergenic
TAAATCCCTGCAAAAGCAAG+AGG	0.610554	4:+51989989	MsG0480021006.01.T01:CDS
AGGTTTGCTGAGCTCAACCA+AGG	0.659807	4:+51990071	MsG0480021006.01.T01:CDS
GAGATGCTTTCATGATTGTG+TGG	0.661772	4:+51989953	MsG0480021006.01.T01:CDS
AAGAAGATGATATATATACT+AGG	0.665897	4:-51986336	None:intergenic
GAGAATGTATAATAACCTGG+TGG	0.692374	4:+51989929	MsG0480021006.01.T01:CDS
TGATATGGAAAATCTTCCAG+AGG	0.718208	4:+51986467	MsG0480021006.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TAATTTATTAGAACTTATAA+CGG	-	Chr4:51989720-51989739	None:intergenic	10.0%
!!!	AAAAAAAACCTAAATTTTTT+TGG	-	Chr4:51986674-51986693	None:intergenic	10.0%
!!	AATAAATTAAGATTTAAACG+TGG	+	Chr4:51987728-51987747	MsG0480021006.01.T01:intron	15.0%
!!	AATAATTTGAGCTTATTTAT+TGG	-	Chr4:51989544-51989563	None:intergenic	15.0%
!!	ACATTAAATAGATCATTAAA+CGG	-	Chr4:51988306-51988325	None:intergenic	15.0%
!!	CTTAATTTATTAGACTTTAT+TGG	-	Chr4:51987719-51987738	None:intergenic	15.0%
!!	TTTGTTTACCAAAAAAATTT+AGG	+	Chr4:51986663-51986682	MsG0480021006.01.T01:intron	15.0%
!!!	AATTTAGGTTTTTTTTTTGT+TGG	+	Chr4:51986678-51986697	MsG0480021006.01.T01:intron	15.0%
!!!	ATAATGTGTTGAATTTATTT+TGG	+	Chr4:51986712-51986731	MsG0480021006.01.T01:intron	15.0%
!!!	TGAATCTTTTTTAAAATTTG+AGG	+	Chr4:51988769-51988788	MsG0480021006.01.T01:intron	15.0%
!!!	TTGAATTTATTTTGGAATTT+GGG	+	Chr4:51986720-51986739	MsG0480021006.01.T01:intron	15.0%
!!	AAATAAATGTACAAACTTTG+AGG	-	Chr4:51988677-51988696	None:intergenic	20.0%
!!	AAGAAGATGATATATATACT+AGG	-	Chr4:51986339-51986358	None:intergenic	20.0%
!!	GTTTAATGATCTATTTAATG+TGG	+	Chr4:51988305-51988324	MsG0480021006.01.T01:intron	20.0%
!!	TCAATATAGTACTTTACTTA+GGG	-	Chr4:51989580-51989599	None:intergenic	20.0%
!!	TGAAGTCTATTAATTAACAT+TGG	+	Chr4:51990110-51990129	MsG0480021006.01.T01:exon	20.0%
!!	TTATTGCTTCATTACATTAT+GGG	+	Chr4:51989804-51989823	MsG0480021006.01.T01:intron	20.0%
!!	TTGGTTTCATAAAATATTAG+TGG	+	Chr4:51987611-51987630	MsG0480021006.01.T01:intron	20.0%
!!!	ATTTTACATATGACTTTGTT+AGG	+	Chr4:51989773-51989792	MsG0480021006.01.T01:intron	20.0%
!!!	CATTTTTGTTTTTGGATAAT+AGG	+	Chr4:51988128-51988147	MsG0480021006.01.T01:intron	20.0%
!!!	CTTTTTTTTCTTTCTTTTGT+TGG	+	Chr4:51988726-51988745	MsG0480021006.01.T01:intron	20.0%
!!!	GCTTTATTATTTACTCATTT+TGG	+	Chr4:51988592-51988611	MsG0480021006.01.T01:intron	20.0%
!!!	GTTGAATTTATTTTGGAATT+TGG	+	Chr4:51986719-51986738	MsG0480021006.01.T01:intron	20.0%
!!!	TGAATCTGTTTTTATATTGA+GGG	+	Chr4:51987894-51987913	MsG0480021006.01.T01:intron	20.0%
!!!	TGAATTTATTTTGGAATTTG+GGG	+	Chr4:51986721-51986740	MsG0480021006.01.T01:intron	20.0%
!!!	TTGAATCTGTTTTTATATTG+AGG	+	Chr4:51987893-51987912	MsG0480021006.01.T01:intron	20.0%
!!!	TTTTACATATGACTTTGTTA+GGG	+	Chr4:51989774-51989793	MsG0480021006.01.T01:intron	20.0%
!!!	TTTTTGTTGTCTATATAATG+TGG	+	Chr4:51987113-51987132	MsG0480021006.01.T01:intron	20.0%
!	AAAAAACCCTAAACTTTACA+AGG	-	Chr4:51986646-51986665	None:intergenic	25.0%
!	AAAATATTAGTGGTGTGTTT+GGG	+	Chr4:51987621-51987640	MsG0480021006.01.T01:intron	25.0%
!	ATAGACAACAAAAATCAATG+TGG	-	Chr4:51987107-51987126	None:intergenic	25.0%
!	CAAATTATCAACATGTCATA+AGG	+	Chr4:51989476-51989495	MsG0480021006.01.T01:intron	25.0%
!	CTCAATATAGTACTTTACTT+AGG	-	Chr4:51989581-51989600	None:intergenic	25.0%
!	GTAAATAATAAAGCTCAATG+TGG	-	Chr4:51988586-51988605	None:intergenic	25.0%
!	GTTATTGCTTCATTACATTA+TGG	+	Chr4:51989803-51989822	MsG0480021006.01.T01:intron	25.0%
!	TAAAATATTAGTGGTGTGTT+TGG	+	Chr4:51987620-51987639	MsG0480021006.01.T01:intron	25.0%
!	TATACCTTAATACAGTCTAT+AGG	+	Chr4:51988435-51988454	MsG0480021006.01.T01:intron	25.0%
!	TATTGCTTCATTACATTATG+GGG	+	Chr4:51989805-51989824	MsG0480021006.01.T01:intron	25.0%
!	TCATGCTAATTTATAAGTTG+CGG	+	Chr4:51987919-51987938	MsG0480021006.01.T01:intron	25.0%
!	TGCTGAAATCTAAATAAGTT+CGG	+	Chr4:51990232-51990251	MsG0480021006.01.T01:three_prime_UTR	25.0%
!	TTCATTCATCTATCACTATT+TGG	+	Chr4:51988409-51988428	MsG0480021006.01.T01:intron	25.0%
!!	AAAAAACGAAGCAATGATTT+TGG	-	Chr4:51986554-51986573	None:intergenic	25.0%
!!	AAAAACGAAGCAATGATTTT+GGG	-	Chr4:51986553-51986572	None:intergenic	25.0%
!!	AATTTAATGAACTAGTCTCT+GGG	-	Chr4:51988241-51988260	None:intergenic	25.0%
!!	AGAGACTAGTTCATTAAATT+GGG	+	Chr4:51988241-51988260	MsG0480021006.01.T01:intron	25.0%
!!!	TTCTAATGAACTAGTCTTTT+GGG	-	Chr4:51987539-51987558	None:intergenic	25.0%
!!!	TTTTAAAATTTGAGGCTTCA+TGG	+	Chr4:51988777-51988796	MsG0480021006.01.T01:intron	25.0%
!!!	TTTTAAGGAGAACATCAATA+TGG	-	Chr4:51988880-51988899	None:intergenic	25.0%
	AAAACTCAATGTGGTAGTTA+AGG	-	Chr4:51987576-51987595	None:intergenic	30.0%
	AAAGTGGTAATTAAACCATG+AGG	-	Chr4:51988271-51988290	None:intergenic	30.0%
	AAATAAATGTACCTCCTTTG+AGG	-	Chr4:51986615-51986634	None:intergenic	30.0%
	AACTAACCCAAAAATTTGCA+AGG	-	Chr4:51987839-51987858	None:intergenic	30.0%
	AATAAATGTACCTCCTTTGA+GGG	-	Chr4:51986614-51986633	None:intergenic	30.0%
	ACGGATAAGAAAAATCAAAG+TGG	-	Chr4:51988287-51988306	None:intergenic	30.0%
	AGAAACATTCAAAAGTCACA+CGG	-	Chr4:51988904-51988923	None:intergenic	30.0%
	AGTTATGACTTAAGCCAATA+TGG	+	Chr4:51988505-51988524	MsG0480021006.01.T01:intron	30.0%
	ATAAGAGCCAATGTCTATAA+CGG	+	Chr4:51987158-51987177	MsG0480021006.01.T01:intron	30.0%
	ATTTGCACCTTGTAAAGTTT+AGG	+	Chr4:51986636-51986655	MsG0480021006.01.T01:intron	30.0%
	CATGCTAATTTATAAGTTGC+GGG	+	Chr4:51987920-51987939	MsG0480021006.01.T01:intron	30.0%
	CATTAGATGAAGGCTTAATT+AGG	+	Chr4:51989016-51989035	MsG0480021006.01.T01:intron	30.0%
	CTTTCTTATCTACTCAATGT+GGG	+	Chr4:51989601-51989620	MsG0480021006.01.T01:intron	30.0%
	GCTGAAATCTAAATAAGTTC+GGG	+	Chr4:51990233-51990252	MsG0480021006.01.T01:three_prime_UTR	30.0%
	TATAATCGTTCGATCCAAAA+TGG	+	Chr4:51986428-51986447	MsG0480021006.01.T01:exon	30.0%
	TCTACCTATAGACTGTATTA+AGG	-	Chr4:51988442-51988461	None:intergenic	30.0%
	TGAAGTTATCATTGTGAGTT+AGG	-	Chr4:51987268-51987287	None:intergenic	30.0%
	TGAAGTTGTTTATCCAAACA+AGG	-	Chr4:51989317-51989336	None:intergenic	30.0%
	TTATTTCCATAAGTTGTCCA+GGG	-	Chr4:51989439-51989458	None:intergenic	30.0%
	TTTGCACCTTGTAAAGTTTA+GGG	+	Chr4:51986637-51986656	MsG0480021006.01.T01:intron	30.0%
!	AATCATGCTTTTGTCCATTT+TGG	-	Chr4:51986445-51986464	None:intergenic	30.0%
!	ATAGTGTTTTCATTGAGAGT+TGG	-	Chr4:51988477-51988496	None:intergenic	30.0%
!	CAAAAGACTAGTTCATTAGA+AGG	+	Chr4:51987538-51987557	MsG0480021006.01.T01:intron	30.0%
!	CAATTTAATGAACTAGTCTC+TGG	-	Chr4:51988242-51988261	None:intergenic	30.0%
!	CAGAGACTAGTTCATTAAAT+TGG	+	Chr4:51988240-51988259	MsG0480021006.01.T01:intron	30.0%
!	CATAGACTAGTTAGTTAGAT+GGG	+	Chr4:51987068-51987087	MsG0480021006.01.T01:intron	30.0%
!	CATCTAACTAACTAGTCTAT+GGG	-	Chr4:51987069-51987088	None:intergenic	30.0%
!	TGACTTTTGAATGTTTCTTC+TGG	+	Chr4:51988906-51988925	MsG0480021006.01.T01:intron	30.0%
!	TTTCCACCTTGCAAATTTTT+GGG	+	Chr4:51987830-51987849	MsG0480021006.01.T01:intron	30.0%
!	TTTTCCACCTTGCAAATTTT+TGG	+	Chr4:51987829-51987848	MsG0480021006.01.T01:intron	30.0%
!	TTTTGATGTCCTCCTTAAAA+TGG	+	Chr4:51988822-51988841	MsG0480021006.01.T01:intron	30.0%
!!	GTCTCTGTCTAAGATTTTTA+TGG	+	Chr4:51988188-51988207	MsG0480021006.01.T01:intron	30.0%
!!	TCTGTCTAAGATTTTTATGG+TGG	+	Chr4:51988191-51988210	MsG0480021006.01.T01:intron	30.0%
!!	TTGGTTTGTATTAGCATTAC+AGG	+	Chr4:51988745-51988764	MsG0480021006.01.T01:intron	30.0%
!!	TTTTAAGGAGAACATCACTA+TGG	-	Chr4:51986816-51986835	None:intergenic	30.0%
!!	TTTTAAGGAGAACGTAACTA+TGG	-	Chr4:51987989-51988008	None:intergenic	30.0%
!!!	CTTCTAATGAACTAGTCTTT+TGG	-	Chr4:51987540-51987559	None:intergenic	30.0%
!!!	GTAGCTGTCATTTTTGTTTT+TGG	+	Chr4:51988120-51988139	MsG0480021006.01.T01:intron	30.0%
!!!	GTTAGTTTTTGTTCTTCTGT+TGG	+	Chr4:51987852-51987871	MsG0480021006.01.T01:intron	30.0%
!!!	TGATTTAGGGTTTTGAGATT+GGG	-	Chr4:51986403-51986422	None:intergenic	30.0%
!!!	TGTCATGTTTTTTGGAGAAT+AGG	+	Chr4:51986953-51986972	MsG0480021006.01.T01:intron	30.0%
	AAATCTTAGACAGAGACCAA+AGG	-	Chr4:51988185-51988204	None:intergenic	35.0%
	AACTTCAATTGTTACGACGT+AGG	+	Chr4:51987281-51987300	MsG0480021006.01.T01:intron	35.0%
	AATGTGGTAGTTAAGGCATA+AGG	-	Chr4:51987569-51987588	None:intergenic	35.0%
	ACCTAATGAACAAGTCTCTA+GGG	-	Chr4:51988540-51988559	None:intergenic	35.0%
	ACGATTATACGCAGTGATTT+AGG	-	Chr4:51986417-51986436	None:intergenic	35.0%
	ACTTCAATTGTTACGACGTA+GGG	+	Chr4:51987282-51987301	MsG0480021006.01.T01:intron	35.0%
	AGAGGCAGATAAACAAAGAA+TGG	+	Chr4:51986485-51986504	MsG0480021006.01.T01:CDS	35.0%
	AGATGAATGAAGCCATGAAA+GGG	-	Chr4:51988400-51988419	None:intergenic	35.0%
	AGTAACCCCTAAAATTTGCA+CGG	-	Chr4:51988708-51988727	None:intergenic	35.0%
	AGTTGACATATTACCAGACA+GGG	+	Chr4:51989872-51989891	MsG0480021006.01.T01:intron	35.0%
	ATAAATGTACCTCCTTTGAG+GGG	-	Chr4:51986613-51986632	None:intergenic	35.0%
	ATAGAACCGAGATTCTTGAT+TGG	+	Chr4:51990280-51990299	MsG0480021006.01.T01:three_prime_UTR	35.0%
	CCAGGTTATTATACATTCTC+AGG	-	Chr4:51989929-51989948	None:intergenic	35.0%
	CCTGAGAATGTATAATAACC+TGG	+	Chr4:51989926-51989945	MsG0480021006.01.T01:CDS	35.0%
	CGATTATACGCAGTGATTTA+GGG	-	Chr4:51986416-51986435	None:intergenic	35.0%
	CTACAGAAAGGAATAGAATG+AGG	-	Chr4:51989192-51989211	None:intergenic	35.0%
	CTCACTAGTTCATTAGATGA+AGG	+	Chr4:51989006-51989025	MsG0480021006.01.T01:intron	35.0%
	CTCATGATAGATTAGTGGTA+TGG	+	Chr4:51989625-51989644	MsG0480021006.01.T01:intron	35.0%
	CTTATTTCCATAAGTTGTCC+AGG	-	Chr4:51989440-51989459	None:intergenic	35.0%
	CTTCATGGTATGTAAGTTGT+TGG	+	Chr4:51988792-51988811	MsG0480021006.01.T01:intron	35.0%
	GAAACATTCAAAAGTCACAC+GGG	-	Chr4:51988903-51988922	None:intergenic	35.0%
	GAGAATGTATAATAACCTGG+TGG	+	Chr4:51989929-51989948	MsG0480021006.01.T01:CDS	35.0%
	GCTTTCTTATCTACTCAATG+TGG	+	Chr4:51989600-51989619	MsG0480021006.01.T01:intron	35.0%
	GTAACAGTCTAGTGTTTGTT+TGG	-	Chr4:51987697-51987716	None:intergenic	35.0%
	GTAGACAAGAAAACTCAATG+TGG	-	Chr4:51987585-51987604	None:intergenic	35.0%
	TAACCCAAAAATTTGCAAGG+TGG	-	Chr4:51987836-51987855	None:intergenic	35.0%
	TACTTTACTTAGGGTCTGTT+GGG	-	Chr4:51989571-51989590	None:intergenic	35.0%
	TAGATGAATGAAGCCATGAA+AGG	-	Chr4:51988401-51988420	None:intergenic	35.0%
	TAGTTGACATATTACCAGAC+AGG	+	Chr4:51989871-51989890	MsG0480021006.01.T01:intron	35.0%
	TATGACTTTGTTAGGGTGAT+TGG	+	Chr4:51989781-51989800	MsG0480021006.01.T01:intron	35.0%
	TCGGCTCTTAAAGTCTTAAA+GGG	-	Chr4:51989081-51989100	None:intergenic	35.0%
	TGAGCTAGGGTTTATCTTTA+CGG	+	Chr4:51986366-51986385	MsG0480021006.01.T01:five_prime_UTR	35.0%
	TGATATGGAAAATCTTCCAG+AGG	+	Chr4:51986467-51986486	MsG0480021006.01.T01:CDS	35.0%
	TTAAGAGCCGACATATACTA+CGG	+	Chr4:51989090-51989109	MsG0480021006.01.T01:intron	35.0%
	TTGTATTAGACTCTCCTTGT+GGG	-	Chr4:51989254-51989273	None:intergenic	35.0%
	TTGTGCTTTCATTGAGAGTT+AGG	-	Chr4:51989215-51989234	None:intergenic	35.0%
	TTTGTATTAGACTCTCCTTG+TGG	-	Chr4:51989255-51989274	None:intergenic	35.0%
!	AATGTTTCTTCTGGACGTTT+TGG	+	Chr4:51988915-51988934	MsG0480021006.01.T01:intron	35.0%
!	ATGCTGTCTTTTAGTACCTT+TGG	+	Chr4:51986991-51987010	MsG0480021006.01.T01:intron	35.0%
!	ATGTTTCTTCTGGACGTTTT+GGG	+	Chr4:51988916-51988935	MsG0480021006.01.T01:intron	35.0%
!	ATTCTTTGTTTATCTGCCTC+TGG	-	Chr4:51986486-51986505	None:intergenic	35.0%
!	CAAAAGCATGATTGCTGATA+TGG	+	Chr4:51986452-51986471	MsG0480021006.01.T01:CDS	35.0%
!	CAAAATCTGCCACCATTTTA+AGG	-	Chr4:51988837-51988856	None:intergenic	35.0%
!	CCATAGACTAGTTAGTTAGA+TGG	+	Chr4:51987067-51987086	MsG0480021006.01.T01:intron	35.0%
!	CCATCTAACTAACTAGTCTA+TGG	-	Chr4:51987070-51987089	None:intergenic	35.0%
!	GGAACTACATTTTCAACAAG+TGG	+	Chr4:51986873-51986892	MsG0480021006.01.T01:intron	35.0%
!	GTGACTTTCAATGTTGCTTA+TGG	+	Chr4:51988014-51988033	MsG0480021006.01.T01:intron	35.0%
!	GTTTGGGTTTCAGTTCAAAA+AGG	+	Chr4:51987637-51987656	MsG0480021006.01.T01:intron	35.0%
!	TGACTATTGAATGTTGCTTC+TGG	+	Chr4:51986842-51986861	MsG0480021006.01.T01:intron	35.0%
!	TGCTGTCTTTTAGTACCTTT+GGG	+	Chr4:51986992-51987011	MsG0480021006.01.T01:intron	35.0%
!	TTATCCTTAGTTCTCCCAAA+CGG	+	Chr4:51989501-51989520	MsG0480021006.01.T01:intron	35.0%
!	TTATGGGGCTCTTGTATTTT+TGG	+	Chr4:51989820-51989839	MsG0480021006.01.T01:intron	35.0%
!	TTTCCACCGTGCAAATTTTA+GGG	+	Chr4:51988699-51988718	MsG0480021006.01.T01:intron	35.0%
!	TTTTCCACCGTGCAAATTTT+AGG	+	Chr4:51988698-51988717	MsG0480021006.01.T01:intron	35.0%
!	TTTTCTTGTCTACTCGATGT+TGG	+	Chr4:51987592-51987611	MsG0480021006.01.T01:intron	35.0%
!!	AAACTTTGAGGCGTAAATCT+GGG	-	Chr4:51988665-51988684	None:intergenic	35.0%
!!	AATGTTGCTTATGGACGTTT+TGG	+	Chr4:51988023-51988042	MsG0480021006.01.T01:intron	35.0%
!!	ATAGACATTGGCTCTTATAG+AGG	-	Chr4:51987156-51987175	None:intergenic	35.0%
!!	ATGTTGCTTATGGACGTTTT+GGG	+	Chr4:51988024-51988043	MsG0480021006.01.T01:intron	35.0%
!!	TACAGCTTTGTTTGAAAGTG+AGG	+	Chr4:51990161-51990180	MsG0480021006.01.T01:three_prime_UTR	35.0%
!!!	CAATAGTCACACTGGTTTTA+AGG	-	Chr4:51986831-51986850	None:intergenic	35.0%
!!!	CTTAGTTGTGTGCTGTTTTT+CGG	+	Chr4:51988157-51988176	MsG0480021006.01.T01:intron	35.0%
!!!	CTTGTAGCTGTCATGTTTTT+TGG	+	Chr4:51986945-51986964	MsG0480021006.01.T01:intron	35.0%
!!!	GTGATTTAGGGTTTTGAGAT+TGG	-	Chr4:51986404-51986423	None:intergenic	35.0%
!!!	TGAAAGTCACACTGGTTTTA+AGG	-	Chr4:51988004-51988023	None:intergenic	35.0%
	AAACAAGGTCTTAAGCCATG+AGG	-	Chr4:51989302-51989321	None:intergenic	40.0%
	AAACACTTGTGAGACCGTTT+GGG	-	Chr4:51989518-51989537	None:intergenic	40.0%
	AAAGGGGAAAGGGATAAAAG+TGG	-	Chr4:51988383-51988402	None:intergenic	40.0%
	AAATGAGTGAACCTGCCAAA+GGG	-	Chr4:51989143-51989162	None:intergenic	40.0%
	AAGGGATAAAAGTGGGTGAA+AGG	-	Chr4:51988375-51988394	None:intergenic	40.0%
	AAGGGGAAAGGGATAAAAGT+GGG	-	Chr4:51988382-51988401	None:intergenic	40.0%
	AATGTGGTACTTAAGTCGTG+AGG	-	Chr4:51988570-51988589	None:intergenic	40.0%
	AGCAACATTGAAAGTCACAC+TGG	-	Chr4:51988012-51988031	None:intergenic	40.0%
	ATTAAATTGGGAGAGCCTCA+TGG	+	Chr4:51988253-51988272	MsG0480021006.01.T01:intron	40.0%
	CAAAATCAGATAACCCTGTC+TGG	-	Chr4:51989888-51989907	None:intergenic	40.0%
	CACCTAATGAACAAGTCTCT+AGG	-	Chr4:51988541-51988560	None:intergenic	40.0%
	CATCAAGTCTAAGAACCTCA+TGG	+	Chr4:51989284-51989303	MsG0480021006.01.T01:intron	40.0%
	CATGACAGCTACAAGTGTTT+AGG	-	Chr4:51986940-51986959	None:intergenic	40.0%
	CCAGAATGAAGAGTTACAGA+GGG	-	Chr4:51989747-51989766	None:intergenic	40.0%
	GAGATGCTTTCATGATTGTG+TGG	+	Chr4:51989953-51989972	MsG0480021006.01.T01:CDS	40.0%
	GATGAATGAAGCCATGAAAG+GGG	-	Chr4:51988399-51988418	None:intergenic	40.0%
	GCAACATTCAATAGTCACAC+TGG	-	Chr4:51986839-51986858	None:intergenic	40.0%
	GTACTTTACTTAGGGTCTGT+TGG	-	Chr4:51989572-51989591	None:intergenic	40.0%
	GTGAAAGCCGTAGTATATGT+CGG	-	Chr4:51989100-51989119	None:intergenic	40.0%
	TAAATCCCTGCAAAAGCAAG+AGG	+	Chr4:51989989-51990008	MsG0480021006.01.T01:CDS	40.0%
	TACCTTTGGGCTCTGTTTAA+TGG	+	Chr4:51987005-51987024	MsG0480021006.01.T01:intron	40.0%
	TAGGGTTAGACTCTCCATAT+TGG	-	Chr4:51988522-51988541	None:intergenic	40.0%
	TCATGGCTTAAGACCTTGTT+TGG	+	Chr4:51989301-51989320	MsG0480021006.01.T01:intron	40.0%
	TCGATGAATGAAACCACCAA+CGG	-	Chr4:51987193-51987212	None:intergenic	40.0%
	TGATGTCCTCCTTAAAATGG+TGG	+	Chr4:51988825-51988844	MsG0480021006.01.T01:intron	40.0%
	TGGGTCTCATGATAGATTAG+TGG	+	Chr4:51989620-51989639	MsG0480021006.01.T01:intron	40.0%
	TGGTGGTTTCATTCATCGAT+CGG	+	Chr4:51987194-51987213	MsG0480021006.01.T01:intron	40.0%
	TGTAGCTTCATCCAACCTAT+AGG	+	Chr4:51987227-51987246	MsG0480021006.01.T01:intron	40.0%
	TGTGAGTTAGGATACAGAGA+GGG	-	Chr4:51987256-51987275	None:intergenic	40.0%
	TTCCATTAAACAGAGCCCAA+AGG	-	Chr4:51987010-51987029	None:intergenic	40.0%
	TTGAGAGTTAGGCTACAGAA+AGG	-	Chr4:51989204-51989223	None:intergenic	40.0%
	TTGTGAGTTAGGATACAGAG+AGG	-	Chr4:51987257-51987276	None:intergenic	40.0%
	TTTATCCCTTTCCCCTTTCA+TGG	+	Chr4:51988385-51988404	MsG0480021006.01.T01:intron	40.0%
!	ACCCTAGAGACTTGTTCATT+AGG	+	Chr4:51988536-51988555	MsG0480021006.01.T01:intron	40.0%
!	CAAAAGTCACACGGGTTTTA+AGG	-	Chr4:51988895-51988914	None:intergenic	40.0%
!	CTAGAGACTTGTTCATTAGG+TGG	+	Chr4:51988539-51988558	MsG0480021006.01.T01:intron	40.0%
!	GTCGGCTCTTAAAGTCTTAA+AGG	-	Chr4:51989082-51989101	None:intergenic	40.0%
!	TAAATGTACCTCCTTTGAGG+GGG	-	Chr4:51986612-51986631	None:intergenic	40.0%
!	TATGTTTGGATGGAGGGTTT+TGG	+	Chr4:51987305-51987324	MsG0480021006.01.T01:intron	40.0%
!	TTCCACCGTGCAAATTTTAG+GGG	+	Chr4:51988700-51988719	MsG0480021006.01.T01:intron	40.0%
!!	AACTTTGAGGCGTAAATCTG+GGG	-	Chr4:51988664-51988683	None:intergenic	40.0%
!!	AATCTGCCACCATTTTAAGG+AGG	-	Chr4:51988834-51988853	None:intergenic	40.0%
!!	AATGTTGCTTCTGGACGTTT+TGG	+	Chr4:51986851-51986870	MsG0480021006.01.T01:intron	40.0%
!!	ATAACGGACTAGAGTGTGTT+TGG	-	Chr4:51989704-51989723	None:intergenic	40.0%
!!	ATGTTGCTTCTGGACGTTTT+GGG	+	Chr4:51986852-51986871	MsG0480021006.01.T01:intron	40.0%
!!	ATTCATCGATCGGTTTTCAG+TGG	+	Chr4:51987204-51987223	MsG0480021006.01.T01:intron	40.0%
!!	CAAACTTTGAGGCGTAAATC+TGG	-	Chr4:51988666-51988685	None:intergenic	40.0%
!!	TTCTGGAGGAGGTTTTTGAA+GGG	-	Chr4:51987491-51987510	None:intergenic	40.0%
!!	TTTCTGGAGGAGGTTTTTGA+AGG	-	Chr4:51987492-51987511	None:intergenic	40.0%
	AAAACACCCTTAAGCTCTCC+AGG	+	Chr4:51987509-51987528	MsG0480021006.01.T01:intron	45.0%
	AACCCCTAAAATTTGCACGG+TGG	-	Chr4:51988705-51988724	None:intergenic	45.0%
	AAGGGAGCCGTTATAGACAT+TGG	-	Chr4:51987168-51987187	None:intergenic	45.0%
	AAGTTGCCCTGGACAACTTA+TGG	+	Chr4:51989430-51989449	MsG0480021006.01.T01:intron	45.0%
	AATGAAACCACCAACGGGAA+AGG	-	Chr4:51987187-51987206	None:intergenic	45.0%
	AATGAGTGAACCTGCCAAAG+GGG	-	Chr4:51989142-51989161	None:intergenic	45.0%
	ATGAAACCACCAACGGGAAA+GGG	-	Chr4:51987186-51987205	None:intergenic	45.0%
	ATGAAGCCATGAAAGGGGAA+AGG	-	Chr4:51988394-51988413	None:intergenic	45.0%
	CAAATGAGTGAACCTGCCAA+AGG	-	Chr4:51989144-51989163	None:intergenic	45.0%
	CCCTCTGTAACTCTTCATTC+TGG	+	Chr4:51989744-51989763	MsG0480021006.01.T01:intron	45.0%
	CGATGAATGAAACCACCAAC+GGG	-	Chr4:51987192-51987211	None:intergenic	45.0%
	CTTCTTCCACACTTGAGCTA+GGG	+	Chr4:51986353-51986372	MsG0480021006.01.T01:five_prime_UTR	45.0%
	GAAACACTTGTGAGACCGTT+TGG	-	Chr4:51989519-51989538	None:intergenic	45.0%
	GATAAACCCTAGCTCAAGTG+TGG	-	Chr4:51986362-51986381	None:intergenic	45.0%
	GCCAGAATGAAGAGTTACAG+AGG	-	Chr4:51989748-51989767	None:intergenic	45.0%
	GTAGGGTATGTTTGGATGGA+GGG	+	Chr4:51987299-51987318	MsG0480021006.01.T01:intron	45.0%
	GTTACGACGTAGGGTATGTT+TGG	+	Chr4:51987291-51987310	MsG0480021006.01.T01:intron	45.0%
	TCTTCTTCCACACTTGAGCT+AGG	+	Chr4:51986352-51986371	MsG0480021006.01.T01:five_prime_UTR	45.0%
	TGAAGCCATGAAAGGGGAAA+GGG	-	Chr4:51988393-51988412	None:intergenic	45.0%
	TGTTATGACTTGAGCCCACA+AGG	+	Chr4:51989237-51989256	MsG0480021006.01.T01:intron	45.0%
!	AAATGTACCTCCTTTGAGGG+GGG	-	Chr4:51986611-51986630	None:intergenic	45.0%
!	AATCTTGAGTTGCAGCTCCT+TGG	-	Chr4:51990091-51990110	None:intergenic	45.0%
!	GATTTGGAGCTGATCGATCA+TGG	-	Chr4:51986514-51986533	None:intergenic	45.0%
!!	AGGAGGTTTTTGAAGGGTTC+TGG	-	Chr4:51987485-51987504	None:intergenic	45.0%
!!	AGGTTTCCTCTTGCTTTTGC+AGG	-	Chr4:51989998-51990017	None:intergenic	45.0%
!!	GACATTGGCTCTTATAGAGG+TGG	-	Chr4:51987153-51987172	None:intergenic	45.0%
!!	GCTATTTTCAGAAGTTGCCC+TGG	+	Chr4:51989419-51989438	MsG0480021006.01.T01:intron	45.0%
!!	GGTTTCCTCTTGCTTTTGCA+GGG	-	Chr4:51989997-51990016	None:intergenic	45.0%
!!!	ACTAGTCTTTTGGGTTCTCC+TGG	-	Chr4:51987530-51987549	None:intergenic	45.0%
!!!	AGCTTAAGGGTGTTTTCTGG+AGG	-	Chr4:51987505-51987524	None:intergenic	45.0%
!!!	GAGAGCTTAAGGGTGTTTTC+TGG	-	Chr4:51987508-51987527	None:intergenic	45.0%
!!!	GGTTTTTGAAGGGTTCTGGA+TGG	-	Chr4:51987481-51987500	None:intergenic	45.0%
!!!	GTGCTGTTTTTCGGTACCTT+TGG	+	Chr4:51988166-51988185	MsG0480021006.01.T01:intron	45.0%
!!!	TTAAGGGTGTTTTCTGGAGG+AGG	-	Chr4:51987502-51987521	None:intergenic	45.0%
!!!	TTTGGATGGAGGGTTTTGGA+GGG	+	Chr4:51987309-51987328	MsG0480021006.01.T01:intron	45.0%
	AACCTCACCTGTCACGGATT+TGG	-	Chr4:51986530-51986549	None:intergenic	50.0%
	ATACAGAGAGGGCTGCCTAT+AGG	-	Chr4:51987245-51987264	None:intergenic	50.0%
	ATACTACGGCTTTCACCTGC+TGG	+	Chr4:51989104-51989123	MsG0480021006.01.T01:intron	50.0%
	ATCAGCTCCAAATCCGTGAC+AGG	+	Chr4:51986520-51986539	MsG0480021006.01.T01:CDS	50.0%
	CGACGTAGGGTATGTTTGGA+TGG	+	Chr4:51987295-51987314	MsG0480021006.01.T01:intron	50.0%
	CGTAGGGTATGTTTGGATGG+AGG	+	Chr4:51987298-51987317	MsG0480021006.01.T01:intron	50.0%
	CTCTGCACATCTTCGAACAC+AGG	-	Chr4:51990018-51990037	None:intergenic	50.0%
	GAGACCGTTTGGGAGAACTA+AGG	-	Chr4:51989508-51989527	None:intergenic	50.0%
	GGTGGTATTCCTTTCCCCTT+TGG	+	Chr4:51989125-51989144	MsG0480021006.01.T01:intron	50.0%
	GGTTCTCCTGGAGAGCTTAA+GGG	-	Chr4:51987518-51987537	None:intergenic	50.0%
	GTATTCCTTTCCCCTTTGGC+AGG	+	Chr4:51989129-51989148	MsG0480021006.01.T01:intron	50.0%
	TTTCTGTTACCCCCCCTCAA+AGG	+	Chr4:51986598-51986617	MsG0480021006.01.T01:intron	50.0%
	TTTGGGAACCTCACCTGTCA+CGG	-	Chr4:51986536-51986555	None:intergenic	50.0%
!	AATGTACCTCCTTTGAGGGG+GGG	-	Chr4:51986610-51986629	None:intergenic	50.0%
!	ACTAGTCTATGGGCTCGACT+CGG	-	Chr4:51987059-51987078	None:intergenic	50.0%
!	ACTCTATGCGTGTTGGCATG+AGG	+	Chr4:51990051-51990070	MsG0480021006.01.T01:CDS	50.0%
!	CTGAAGCACTCTATGCGTGT+TGG	+	Chr4:51990044-51990063	MsG0480021006.01.T01:CDS	50.0%
!!	AGGTTTGCTGAGCTCAACCA+AGG	+	Chr4:51990071-51990090	MsG0480021006.01.T01:CDS	50.0%
!!	CATGAAAGCATCTCTCCACC+AGG	-	Chr4:51989947-51989966	None:intergenic	50.0%
!!	GTTTGGATGGAGGGTTTTGG+AGG	+	Chr4:51987308-51987327	MsG0480021006.01.T01:intron	50.0%
!!	TGAAGGGTTCTGGATGGCTA+AGG	-	Chr4:51987475-51987494	None:intergenic	50.0%
!!!	TTGGATGGAGGGTTTTGGAG+GGG	+	Chr4:51987310-51987329	MsG0480021006.01.T01:intron	50.0%
	AGAGAGGGCTGCCTATAGGT+TGG	-	Chr4:51987241-51987260	None:intergenic	55.0%
	ATAACGGCTCCCTTTCCCGT+TGG	+	Chr4:51987174-51987193	MsG0480021006.01.T01:intron	55.0%
	CTCCAAATCCGTGACAGGTG+AGG	+	Chr4:51986525-51986544	MsG0480021006.01.T01:intron	55.0%
	GGGAAAGGAATACCACCAGC+AGG	-	Chr4:51989122-51989141	None:intergenic	55.0%
	GGGTTCTCCTGGAGAGCTTA+AGG	-	Chr4:51987519-51987538	None:intergenic	55.0%
	GTGAACCTGCCAAAGGGGAA+AGG	-	Chr4:51989137-51989156	None:intergenic	55.0%
	CTACGGCTTTCACCTGCTGG+TGG	+	Chr4:51989107-51989126	MsG0480021006.01.T01:intron	60.0%
	CTGTTACCCCCCCTCAAAGG+AGG	+	Chr4:51986601-51986620	MsG0480021006.01.T01:intron	60.0%
!!!	GATGGAGGGTTTTGGAGGGG+AGG	+	Chr4:51987313-51987332	MsG0480021006.01.T01:intron	60.0%
	ACGGCTCCCTTTCCCGTTGG+TGG	+	Chr4:51987177-51987196	MsG0480021006.01.T01:intron	65.0%

Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	51986332	51990301	51986332	ID=MsG0480021006.01;Name=MsG0480021006.01
Chr4	mRNA	51986332	51990301	51986332	ID=MsG0480021006.01.T01;Parent=MsG0480021006.01;Name=MsG0480021006.01.T01;_AED=0.48;_eAED=0.48;_QI=115\|1\|1\|1\|1\|1\|2\|189\|93
Chr4	exon	51986332	51986541	51986332	ID=MsG0480021006.01.T01:exon:7879;Parent=MsG0480021006.01.T01
Chr4	exon	51989926	51990301	51989926	ID=MsG0480021006.01.T01:exon:7880;Parent=MsG0480021006.01.T01
Chr4	five_prime_UTR	51986332	51986446	51986332	ID=MsG0480021006.01.T01:five_prime_utr;Parent=MsG0480021006.01.T01
Chr4	CDS	51986447	51986541	51986447	ID=MsG0480021006.01.T01:cds;Parent=MsG0480021006.01.T01
Chr4	CDS	51989926	51990112	51989926	ID=MsG0480021006.01.T01:cds;Parent=MsG0480021006.01.T01
Chr4	three_prime_UTR	51990113	51990301	51990113	ID=MsG0480021006.01.T01:three_prime_utr;Parent=MsG0480021006.01.T01

Gene Sequence	>MsG0480021006.01.T01 ATGGACAAAAGCATGATTGCTGATATGGAAAATCTTCCAGAGGCAGATAAACAAAGAATGGCTACCATGATCGATCAGCTCCAAATCCGTGACAGCCTGAGAATGTATAATAACCTGGTGGAGAGATGCTTTCATGATTGTGTGGATACATTTAAGCATAAATCCCTGCAAAAGCAAGAGGAAACCTGTGTTCGAAGATGTGCAGAGAAATTTCTGAAGCACTCTATGCGTGTTGGCATGAGGTTTGCTGAGCTCAACCAAGGAGCTGCAACTCAAGATTGA
Protein sequence	>MsG0480021006.01.T01 MDKSMIADMENLPEADKQRMATMIDQLQIRDSLRMYNNLVERCFHDCVDTFKHKSLQKQEETCVRRCAEKFLKHSMRVGMRFAELNQGAATQD*