AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880047470.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880047470.01.T01 MTR_7g029510 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0880047470.01.T01 MTR_7g099610 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0880047470.01.T01 MTR_5g063620 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0880047470.01.T01 MTR_4g061827 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0880047470.01.T01 MTR_4g061920 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0880047470.01.T01 MTR_3g462400 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0880047470.01.T01 MTR_3g054380 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0880047470.01.T01 MTR_3g462730 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0880047470.01.T01 MTR_3g462510 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0880047470.01.T01 MTR_3g070920 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0880047470.01.T01 MTR_3g061440 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0880047470.01.T01 MTR_3g462410 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0880047470.01.T01 MTR_2g096100 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0880047470.01.T01 MTR_8g038460 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0880047470.01.T01 MTR_4g128150 100.000 102 0 0 1 102 1 102 1.74e-66 204
MsG0880047470.01.T01 MTR_7g029520 84.000 50 8 0 27 76 1 50 2.23e-21 87.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880047470.01.T01 AT5G59970 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0880047470.01.T01 AT5G59690 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0880047470.01.T01 AT5G59970 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0880047470.01.T01 AT3G46320 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0880047470.01.T01 AT3G45930 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0880047470.01.T01 AT3G53730 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0880047470.01.T01 AT2G28740 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0880047470.01.T01 AT1G07660 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0880047470.01.T01 AT1G07820 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0880047470.01.T01 AT1G07820 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0880047470.01.T01 AT1G07660 83.495 103 0 1 1 103 1 86 4.52e-51 155

Find 27 sgRNAs with CRISPR-Local

Find 26 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCAAAGGAGGAAAGGGTTT+AGG 0.384869 8:+86851687 MsG0880047470.01.T01:CDS
CTAGCGAGACGACGAATTGC+AGG 0.420434 8:-86851771 None:intergenic
GGAGGAAAGGGTTTAGGAAA+GGG 0.429069 8:+86851693 MsG0880047470.01.T01:CDS
GGAAGGACTCTTTACGGATT+CGG 0.436444 8:+86851957 MsG0880047470.01.T01:CDS
AGGCAGGGAAGGACTCTTTA+CGG 0.443617 8:+86851951 MsG0880047470.01.T01:CDS
TTGCAGGCTTTGTAATTCCC+TGG 0.457591 8:-86851755 None:intergenic
TGTTTACGCTCTCAAGAGGC+AGG 0.493747 8:+86851935 MsG0880047470.01.T01:CDS
AGGAAGAGGCAAAGGAGGAA+AGG 0.518412 8:+86851680 MsG0880047470.01.T01:CDS
GGTTCTTCGTGATAACATCC+AGG 0.522451 8:+86851737 MsG0880047470.01.T01:CDS
GGAAGAGGCAAAGGAGGAAA+GGG 0.527098 8:+86851681 MsG0880047470.01.T01:CDS
AGGAGGAAAGGGTTTAGGAA+AGG 0.528235 8:+86851692 MsG0880047470.01.T01:CDS
GGAAAGGGTTTAGGAAAGGG+AGG 0.536831 8:+86851696 MsG0880047470.01.T01:CDS
CCGTAAAACTGTTACTGCGA+TGG 0.549023 8:+86851908 MsG0880047470.01.T01:CDS
GCGATGTCAGGAAGAGGCAA+AGG 0.557914 8:+86851672 None:intergenic
TGTTATCACGAAGAACCTTA+CGG 0.569497 8:-86851731 None:intergenic
TTCACAGCGATGTCAGGAAG+AGG 0.573275 8:+86851666 None:intergenic
AGGAGCAAAACGACACCGTA+AGG 0.575994 8:+86851716 MsG0880047470.01.T01:CDS
ACGCTCTCAAGAGGCAGGGA+AGG 0.583359 8:+86851940 MsG0880047470.01.T01:CDS
ATGTCAGGAAGAGGCAAAGG+AGG 0.592835 8:+86851675 MsG0880047470.01.T01:CDS
AGGACTCTTTACGGATTCGG+TGG 0.600535 8:+86851960 MsG0880047470.01.T01:CDS
ATGTTGTTTACGCTCTCAAG+AGG 0.616421 8:+86851931 MsG0880047470.01.T01:CDS
GTTTACGCTCTCAAGAGGCA+GGG 0.624823 8:+86851936 MsG0880047470.01.T01:CDS
GTTCTTCGTGATAACATCCA+GGG 0.626731 8:+86851738 MsG0880047470.01.T01:CDS
TTAATCTATGAAGAAACTCG+TGG 0.634992 8:+86851822 MsG0880047470.01.T01:CDS
ATTCGTCGTCTCGCTAGACG+TGG 0.638162 8:+86851777 MsG0880047470.01.T01:CDS
CGTCGTCTCGCTAGACGTGG+TGG 0.639871 8:+86851780 MsG0880047470.01.T01:CDS
GGTGGTGTGAAGCGTATCAG+TGG 0.669421 8:+86851798 MsG0880047470.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
TTAATCTATGAAGAAACTCG+TGG + Chr8:86851822-86851841 MsG0880047470.01.T01:CDS 30.0%
TGTTATCACGAAGAACCTTA+CGG - Chr8:86851734-86851753 None:intergenic 35.0%
GTTCTTCGTGATAACATCCA+GGG + Chr8:86851738-86851757 MsG0880047470.01.T01:CDS 40.0%
! ATGTTGTTTACGCTCTCAAG+AGG + Chr8:86851931-86851950 MsG0880047470.01.T01:CDS 40.0%
! CCATCGCAGTAACAGTTTTA+CGG - Chr8:86851911-86851930 None:intergenic 40.0%
AGGAGGAAAGGGTTTAGGAA+AGG + Chr8:86851692-86851711 MsG0880047470.01.T01:CDS 45.0%
CCGTAAAACTGTTACTGCGA+TGG + Chr8:86851908-86851927 MsG0880047470.01.T01:CDS 45.0%
GGAAGGACTCTTTACGGATT+CGG + Chr8:86851957-86851976 MsG0880047470.01.T01:CDS 45.0%
GGAGGAAAGGGTTTAGGAAA+GGG + Chr8:86851693-86851712 MsG0880047470.01.T01:CDS 45.0%
GGTTCTTCGTGATAACATCC+AGG + Chr8:86851737-86851756 MsG0880047470.01.T01:CDS 45.0%
! TTGCAGGCTTTGTAATTCCC+TGG - Chr8:86851758-86851777 None:intergenic 45.0%
AGGAAGAGGCAAAGGAGGAA+AGG + Chr8:86851680-86851699 MsG0880047470.01.T01:CDS 50.0%
AGGACTCTTTACGGATTCGG+TGG + Chr8:86851960-86851979 MsG0880047470.01.T01:CDS 50.0%
AGGAGCAAAACGACACCGTA+AGG + Chr8:86851716-86851735 MsG0880047470.01.T01:CDS 50.0%
AGGCAGGGAAGGACTCTTTA+CGG + Chr8:86851951-86851970 MsG0880047470.01.T01:CDS 50.0%
ATGTCAGGAAGAGGCAAAGG+AGG + Chr8:86851675-86851694 MsG0880047470.01.T01:CDS 50.0%
GGAAAGGGTTTAGGAAAGGG+AGG + Chr8:86851696-86851715 MsG0880047470.01.T01:CDS 50.0%
GGAAGAGGCAAAGGAGGAAA+GGG + Chr8:86851681-86851700 MsG0880047470.01.T01:CDS 50.0%
GGCAAAGGAGGAAAGGGTTT+AGG + Chr8:86851687-86851706 MsG0880047470.01.T01:CDS 50.0%
GTTTACGCTCTCAAGAGGCA+GGG + Chr8:86851936-86851955 MsG0880047470.01.T01:CDS 50.0%
TGTTTACGCTCTCAAGAGGC+AGG + Chr8:86851935-86851954 MsG0880047470.01.T01:CDS 50.0%
ATTCGTCGTCTCGCTAGACG+TGG + Chr8:86851777-86851796 MsG0880047470.01.T01:CDS 55.0%
CTAGCGAGACGACGAATTGC+AGG - Chr8:86851774-86851793 None:intergenic 55.0%
GGTGGTGTGAAGCGTATCAG+TGG + Chr8:86851798-86851817 MsG0880047470.01.T01:CDS 55.0%
ACGCTCTCAAGAGGCAGGGA+AGG + Chr8:86851940-86851959 MsG0880047470.01.T01:CDS 60.0%
CGTCGTCTCGCTAGACGTGG+TGG + Chr8:86851780-86851799 MsG0880047470.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr8 gene 86851675 86851986 86851675 ID=MsG0880047470.01;Name=MsG0880047470.01
Chr8 mRNA 86851675 86851986 86851675 ID=MsG0880047470.01.T01;Parent=MsG0880047470.01;Name=MsG0880047470.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|103
Chr8 exon 86851675 86851986 86851675 ID=MsG0880047470.01.T01:exon:17950;Parent=MsG0880047470.01.T01
Chr8 CDS 86851675 86851986 86851675 ID=MsG0880047470.01.T01:cds;Parent=MsG0880047470.01.T01
Gene Sequence

>MsG0880047470.01.T01

ATGTCAGGAAGAGGCAAAGGAGGAAAGGGTTTAGGAAAGGGAGGAGCAAAACGACACCGTAAGGTTCTTCGTGATAACATCCAGGGAATTACAAAGCCTGCAATTCGTCGTCTCGCTAGACGTGGTGGTGTGAAGCGTATCAGTGGCTTAATCTATGAAGAAACTCGTGGTGTTCTCAAAATCTTTCTTGAGAACGTGATTCGTGATGCTGTCACATACACTGAACATGCTCGCCGTAAAACTGTTACTGCGATGGATGTTGTTTACGCTCTCAAGAGGCAGGGAAGGACTCTTTACGGATTCGGTGGTTAG

Protein sequence

>MsG0880047470.01.T01

MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG*