AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780038625.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780038625.01.T01 MTR_7g058640 85.930 199 16 1 1 199 1 187 5.11e-125 352
MsG0780038625.01.T01 MTR_7g058640 84.574 188 17 1 1 188 1 176 2.45e-115 326
MsG0780038625.01.T01 MTR_4g022570 68.342 199 51 1 1 199 1 187 7.52e-98 283
MsG0780038625.01.T01 MTR_1g047720 62.814 199 62 1 1 199 1 187 2.46e-92 269
MsG0780038625.01.T01 MTR_7g058900 64.824 199 58 1 1 199 1 187 7.40e-91 265
MsG0780038625.01.T01 MTR_4g083490 59.898 197 67 1 3 199 5 189 1.41e-86 254
MsG0780038625.01.T01 MTR_2g028790 60.406 197 66 1 3 199 7 191 1.37e-84 249
MsG0780038625.01.T01 MTR_2g436870 60.302 199 67 1 1 199 1 187 6.38e-84 248
MsG0780038625.01.T01 MTR_7g058640 90.826 109 10 0 91 199 1 109 6.41e-69 207
MsG0780038625.01.T01 MTR_1g111890 46.789 218 85 3 1 199 1 206 8.59e-68 207
MsG0780038625.01.T01 MTR_1g047710 62.595 131 47 1 69 199 7 135 1.82e-57 177
MsG0780038625.01.T01 MTR_4g022470 67.273 110 36 0 73 182 7 116 2.21e-50 159
MsG0780038625.01.T01 MTR_5g089370 32.642 193 115 4 5 196 2 180 1.09e-26 101
MsG0780038625.01.T01 MTR_4g052550 29.082 196 124 4 5 199 2 183 2.11e-26 101
MsG0780038625.01.T01 MTR_4g115390 29.744 195 126 6 6 199 3 187 1.67e-19 83.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780038625.01.T01 AT2G33120 66.834 199 62 2 1 199 1 195 3.14e-95 277
MsG0780038625.01.T01 AT2G33120 66.332 199 55 1 1 199 1 187 1.52e-93 272
MsG0780038625.01.T01 AT1G04750 65.829 199 56 1 1 199 1 187 3.51e-93 271
MsG0780038625.01.T01 AT1G04760 63.819 199 60 1 1 199 1 187 3.21e-92 269
MsG0780038625.01.T01 AT1G04760 63.819 199 60 1 1 199 1 187 3.21e-92 269
MsG0780038625.01.T01 AT2G32670 64.322 199 59 1 1 199 66 252 1.89e-91 269
MsG0780038625.01.T01 AT4G15780 58.763 194 67 2 1 193 1 182 1.71e-78 233
MsG0780038625.01.T01 AT2G33110 54.040 198 75 2 1 198 1 182 1.32e-68 209
MsG0780038625.01.T01 AT3G54300 47.273 220 81 3 1 199 1 206 3.55e-68 208
MsG0780038625.01.T01 AT3G54300 47.273 220 81 3 1 199 1 206 3.55e-68 208
MsG0780038625.01.T01 AT3G54300 47.273 220 81 3 1 199 1 206 3.55e-68 208
MsG0780038625.01.T01 AT2G33110 58.046 174 61 1 1 174 1 162 1.13e-67 206
MsG0780038625.01.T01 AT1G04750 66.972 109 36 0 91 199 1 109 1.19e-48 155
MsG0780038625.01.T01 AT1G04750 66.972 109 36 0 91 199 1 109 1.19e-48 155
MsG0780038625.01.T01 AT1G04750 66.972 109 36 0 91 199 1 109 1.19e-48 155
MsG0780038625.01.T01 AT5G11150 30.964 197 120 4 5 199 2 184 1.66e-26 101
MsG0780038625.01.T01 AT5G11150 30.964 197 120 4 5 199 2 184 2.69e-26 100
MsG0780038625.01.T01 AT5G22360 28.934 197 124 4 5 199 2 184 1.27e-25 99.4
MsG0780038625.01.T01 AT4G32150 30.570 193 119 4 5 196 2 180 4.29e-24 95.1
MsG0780038625.01.T01 AT2G25340 30.319 188 116 4 5 191 2 175 6.62e-23 92.0
MsG0780038625.01.T01 AT2G33120 56.667 60 26 0 140 199 46 105 3.18e-13 64.7

Find 31 sgRNAs with CRISPR-Local

Find 74 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAAGCTTGAACAAAGAATT+TGG 0.149954 7:+50082950 MsG0780038625.01.T01:CDS
AGCTATTTCAGCAAAGTTTC+CGG 0.255031 7:-50082433 None:intergenic
GCAAGTTTGCTAATCTCTTC+TGG 0.255298 7:-50083115 None:intergenic
GGCGCGTGGCACGGTGATTC+TGG 0.320729 7:+50082392 MsG0780038625.01.T01:CDS
TGGTTGCTGGGAGTCTTTGC+AGG 0.323885 7:-50082464 None:intergenic
TCCGGTGAAGTTGGTGTGCT+CGG 0.407070 7:-50082415 None:intergenic
AACTGACAACCTTCGTGATC+AGG 0.429765 7:+50083329 MsG0780038625.01.T01:CDS
AAAGCTTGAACAAAGAATTT+GGG 0.431429 7:+50082951 MsG0780038625.01.T01:CDS
ATCACTTATATACAGCTTTG+TGG 0.443785 7:+50082371 MsG0780038625.01.T01:CDS
CTCCTTGATGCGATCAAGAA+AGG 0.482399 7:-50082879 None:intergenic
AGCAAAGTTTCCGGTGAAGT+TGG 0.500993 7:-50082424 None:intergenic
CAGCTTTGTGGCGCGTGGCA+CGG 0.506415 7:+50082383 MsG0780038625.01.T01:CDS
GGCAAGTACTGAATCCATCA+TGG 0.518155 7:-50082536 None:intergenic
GCCGAGCACACCAACTTCAC+CGG 0.518367 7:+50082414 MsG0780038625.01.T01:CDS
TCAAGTTCTAGATGTTATGT+TGG 0.518679 7:+50083159 MsG0780038625.01.T01:CDS
GGCCTTTCTTGATCGCATCA+AGG 0.526618 7:+50082877 MsG0780038625.01.T01:CDS
AGCTTACTGCGTTGTTGCTG+TGG 0.527710 7:+50082829 MsG0780038625.01.T01:CDS
TTCAACTACCTCGCCCATGA+TGG 0.533919 7:+50082522 MsG0780038625.01.T01:CDS
ATCATGGGCGAGGTAGTTGA+AGG 0.542753 7:-50082520 None:intergenic
TACTGAATCCATCATGGGCG+AGG 0.571090 7:-50082530 None:intergenic
ACATGATCGTCATATTGCTA+TGG 0.577485 7:+50082799 MsG0780038625.01.T01:CDS
ACTTGCGAAAGTAAAAGCTC+AGG 0.586038 7:+50083132 MsG0780038625.01.T01:CDS
ATATACAGCTTTGTGGCGCG+TGG 0.589934 7:+50082378 MsG0780038625.01.T01:CDS
ATGGTTTAGACCGAAAATGA+AGG 0.599090 7:+50083066 MsG0780038625.01.T01:intron
GTAGTTGAAGGTATGACCAT+CGG 0.631667 7:-50082508 None:intergenic
GCAAGTACTGAATCCATCAT+GGG 0.632742 7:-50082535 None:intergenic
CTTTCTTGATCGCATCAAGG+AGG 0.649404 7:+50082880 MsG0780038625.01.T01:CDS
AAATTCACTTACAACACCGA+TGG 0.653817 7:+50082492 MsG0780038625.01.T01:CDS
TTTACCAAAAGATATGCTGG+AGG 0.669628 7:+50082905 MsG0780038625.01.T01:CDS
GGAGCATATGCAATATTGTG+TGG 0.672159 7:+50083087 MsG0780038625.01.T01:CDS
ATAAAAGGACCTGATCACGA+AGG 0.753478 7:-50083338 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAAGAAAAAAATAAAACG+AGG - Chr7:50082560-50082579 None:intergenic 15.0%
!! TTATATTATATATGGTTACT+AGG - Chr7:50083494-50083513 None:intergenic 15.0%
!! TTTATGTATGAACAAATAAA+AGG - Chr7:50083356-50083375 None:intergenic 15.0%
!!! AATTTTCTCTTTTAATCTAA+CGG - Chr7:50083249-50083268 None:intergenic 15.0%
!!! TGAAGTTTTTTTATTCTATA+TGG + Chr7:50083043-50083062 MsG0780038625.01.T01:intron 15.0%
!! TTATAGTTATGTAACTGATA+TGG + Chr7:50083563-50083582 MsG0780038625.01.T01:intron 20.0%
!!! GTTTTTTTATTCTATATGGA+TGG + Chr7:50083047-50083066 MsG0780038625.01.T01:intron 20.0%
!!! TCTTTTACATAATCTAATTG+TGG + Chr7:50082573-50082592 MsG0780038625.01.T01:intron 20.0%
! AAAAGCTTGAACAAAGAATT+TGG + Chr7:50082950-50082969 MsG0780038625.01.T01:CDS 25.0%
! AAAGCTTGAACAAAGAATTT+GGG + Chr7:50082951-50082970 MsG0780038625.01.T01:CDS 25.0%
! AGGTATGTATATGTCTAAAT+TGG + Chr7:50083194-50083213 MsG0780038625.01.T01:intron 25.0%
! ATACATAAACTGTGTTCATT+TGG + Chr7:50083367-50083386 MsG0780038625.01.T01:intron 25.0%
! ATGTGAGTCATTTATTTCAA+AGG - Chr7:50083439-50083458 None:intergenic 25.0%
! ATTATATATGGTTACTAGGA+TGG - Chr7:50083490-50083509 None:intergenic 25.0%
! CAAATCATAAATTTCCTTAG+AGG - Chr7:50082726-50082745 None:intergenic 25.0%
! TAACTGATATGGTTTGAATA+TGG + Chr7:50083574-50083593 MsG0780038625.01.T01:intron 25.0%
!!! TATGTTGGAAAATTTTGATC+AGG + Chr7:50083174-50083193 MsG0780038625.01.T01:CDS 25.0%
AATAAATGACTCACATCACA+TGG + Chr7:50083443-50083462 MsG0780038625.01.T01:intron 30.0%
ATCACTTATATACAGCTTTG+TGG + Chr7:50082371-50082390 MsG0780038625.01.T01:CDS 30.0%
TCAAATGCATTTACATGTAC+TGG + Chr7:50082679-50082698 MsG0780038625.01.T01:intron 30.0%
! GATTTTACCAAAAGATATGC+TGG + Chr7:50082902-50082921 MsG0780038625.01.T01:CDS 30.0%
! GTTGTAAGTGAATTTTGTGT+TGG - Chr7:50082487-50082506 None:intergenic 30.0%
! TTTTGATCGCAATATTGCTA+TGG + Chr7:50082856-50082875 MsG0780038625.01.T01:CDS 30.0%
!! TCAAGTTCTAGATGTTATGT+TGG + Chr7:50083159-50083178 MsG0780038625.01.T01:CDS 30.0%
AAATTCACTTACAACACCGA+TGG + Chr7:50082492-50082511 MsG0780038625.01.T01:CDS 35.0%
ACATGATCGTCATATTGCTA+TGG + Chr7:50082799-50082818 MsG0780038625.01.T01:CDS 35.0%
ATGGTTTAGACCGAAAATGA+AGG + Chr7:50083066-50083085 MsG0780038625.01.T01:intron 35.0%
TATGTCTAAATTGGATCCTC+TGG + Chr7:50083203-50083222 MsG0780038625.01.T01:intron 35.0%
TCAATGCAATGCGATATATG+AGG + Chr7:50082605-50082624 MsG0780038625.01.T01:intron 35.0%
TTTACCAAAAGATATGCTGG+AGG + Chr7:50082905-50082924 MsG0780038625.01.T01:CDS 35.0%
! AGCTATTTCAGCAAAGTTTC+CGG - Chr7:50082436-50082455 None:intergenic 35.0%
! TGCATATGCTCCTTCATTTT+CGG - Chr7:50083079-50083098 None:intergenic 35.0%
! TTTTCCTCCAGCATATCTTT+TGG - Chr7:50082912-50082931 None:intergenic 35.0%
!! TACATGTACTGGTTTCATGT+CGG + Chr7:50082690-50082709 MsG0780038625.01.T01:intron 35.0%
!!! TGAATTTTGTGTTGGTTGCT+GGG - Chr7:50082479-50082498 None:intergenic 35.0%
ACTTGCGAAAGTAAAAGCTC+AGG + Chr7:50083132-50083151 MsG0780038625.01.T01:CDS 40.0%
ATAAAAGGACCTGATCACGA+AGG - Chr7:50083341-50083360 None:intergenic 40.0%
GGAGCATATGCAATATTGTG+TGG + Chr7:50083087-50083106 MsG0780038625.01.T01:CDS 40.0%
! AGTGAAGATCGACGTTTTGA+TGG + Chr7:50083302-50083321 MsG0780038625.01.T01:CDS 40.0%
! GTAGTTGAAGGTATGACCAT+CGG - Chr7:50082511-50082530 None:intergenic 40.0%
! TGGTTTGAATATGGCTTTGC+AGG + Chr7:50083583-50083602 MsG0780038625.01.T01:intron 40.0%
! TTTGCAGGCACAAGTTTTCA+GGG + Chr7:50083598-50083617 MsG0780038625.01.T01:intron 40.0%
!! GCAAGTACTGAATCCATCAT+GGG - Chr7:50082538-50082557 None:intergenic 40.0%
!! GCAAGTTTGCTAATCTCTTC+TGG - Chr7:50083118-50083137 None:intergenic 40.0%
!!! GTGAATTTTGTGTTGGTTGC+TGG - Chr7:50082480-50082499 None:intergenic 40.0%
AACGGTCTGCACTTCAAACT+TGG - Chr7:50083231-50083250 None:intergenic 45.0%
AACTGACAACCTTCGTGATC+AGG + Chr7:50083329-50083348 MsG0780038625.01.T01:CDS 45.0%
AGCAAAGTTTCCGGTGAAGT+TGG - Chr7:50082427-50082446 None:intergenic 45.0%
CTCCTTGATGCGATCAAGAA+AGG - Chr7:50082882-50082901 None:intergenic 45.0%
CTTTCTTGATCGCATCAAGG+AGG + Chr7:50082880-50082899 MsG0780038625.01.T01:CDS 45.0%
GCAATGCGATATATGAGGCA+CGG + Chr7:50082610-50082629 MsG0780038625.01.T01:intron 45.0%
TCGGACATTGAACACCTCTA+AGG + Chr7:50082709-50082728 MsG0780038625.01.T01:intron 45.0%
! CTTTGCAGGCACAAGTTTTC+AGG + Chr7:50083597-50083616 MsG0780038625.01.T01:intron 45.0%
! TTGCAGGCACAAGTTTTCAG+GGG + Chr7:50083599-50083618 MsG0780038625.01.T01:intron 45.0%
!! GGCAAGTACTGAATCCATCA+TGG - Chr7:50082539-50082558 None:intergenic 45.0%
!! TAATTTAGTTATATTATATA+TGG - Chr7:50083502-50083521 None:intergenic 5.0%
AGCTTACTGCGTTGTTGCTG+TGG + Chr7:50082829-50082848 MsG0780038625.01.T01:CDS 50.0%
ATCATGGGCGAGGTAGTTGA+AGG - Chr7:50082523-50082542 None:intergenic 50.0%
CACTTCAAACTTGGCTCCAG+AGG - Chr7:50083222-50083241 None:intergenic 50.0%
GGCCTTTCTTGATCGCATCA+AGG + Chr7:50082877-50082896 MsG0780038625.01.T01:CDS 50.0%
TACTGAATCCATCATGGGCG+AGG - Chr7:50082533-50082552 None:intergenic 50.0%
TTCAACTACCTCGCCCATGA+TGG + Chr7:50082522-50082541 MsG0780038625.01.T01:CDS 50.0%
! ATATACAGCTTTGTGGCGCG+TGG + Chr7:50082378-50082397 MsG0780038625.01.T01:CDS 50.0%
!! TGCAGGCACAAGTTTTCAGG+GGG + Chr7:50083600-50083619 MsG0780038625.01.T01:intron 50.0%
TGGTTGCTGGGAGTCTTTGC+AGG - Chr7:50082467-50082486 None:intergenic 55.0%
!! AAGTTTTCAGGGGGCAGGGA+GGG + Chr7:50083609-50083628 MsG0780038625.01.T01:CDS 55.0%
!! GCACAAGTTTTCAGGGGGCA+GGG + Chr7:50083605-50083624 MsG0780038625.01.T01:CDS 55.0%
!! TCCGGTGAAGTTGGTGTGCT+CGG - Chr7:50082418-50082437 None:intergenic 55.0%
GCCGAGCACACCAACTTCAC+CGG + Chr7:50082414-50082433 MsG0780038625.01.T01:CDS 60.0%
!! CAAGTTTTCAGGGGGCAGGG+AGG + Chr7:50083608-50083627 MsG0780038625.01.T01:CDS 60.0%
!! GGCACAAGTTTTCAGGGGGC+AGG + Chr7:50083604-50083623 MsG0780038625.01.T01:intron 60.0%
GGGGGCAGGGAGGGCAATTA+AGG + Chr7:50083618-50083637 MsG0780038625.01.T01:CDS 65.0%
! CAGCTTTGTGGCGCGTGGCA+CGG + Chr7:50082383-50082402 MsG0780038625.01.T01:CDS 65.0%
GGCGCGTGGCACGGTGATTC+TGG + Chr7:50082392-50082411 MsG0780038625.01.T01:CDS 70.0%
Chromosome Type Strat End Strand Name
Chr7 gene 50082360 50083652 50082360 ID=MsG0780038625.01;Name=MsG0780038625.01
Chr7 mRNA 50082360 50083652 50082360 ID=MsG0780038625.01.T01;Parent=MsG0780038625.01;Name=MsG0780038625.01.T01;_AED=0.49;_eAED=0.49;_QI=0|0|0|1|1|1|5|0|199
Chr7 exon 50082360 50082549 50082360 ID=MsG0780038625.01.T01:exon:5546;Parent=MsG0780038625.01.T01
Chr7 exon 50082795 50082972 50082795 ID=MsG0780038625.01.T01:exon:5547;Parent=MsG0780038625.01.T01
Chr7 exon 50083075 50083195 50083075 ID=MsG0780038625.01.T01:exon:5548;Parent=MsG0780038625.01.T01
Chr7 exon 50083288 50083350 50083288 ID=MsG0780038625.01.T01:exon:5549;Parent=MsG0780038625.01.T01
Chr7 exon 50083605 50083652 50083605 ID=MsG0780038625.01.T01:exon:5550;Parent=MsG0780038625.01.T01
Chr7 CDS 50082360 50082549 50082360 ID=MsG0780038625.01.T01:cds;Parent=MsG0780038625.01.T01
Chr7 CDS 50082795 50082972 50082795 ID=MsG0780038625.01.T01:cds;Parent=MsG0780038625.01.T01
Chr7 CDS 50083075 50083195 50083075 ID=MsG0780038625.01.T01:cds;Parent=MsG0780038625.01.T01
Chr7 CDS 50083288 50083350 50083288 ID=MsG0780038625.01.T01:cds;Parent=MsG0780038625.01.T01
Chr7 CDS 50083605 50083652 50083605 ID=MsG0780038625.01.T01:cds;Parent=MsG0780038625.01.T01
Gene Sequence

>MsG0780038625.01.T01

ATGGCGCAACAATCACTTATATACAGCTTTGTGGCGCGTGGCACGGTGATTCTGGCCGAGCACACCAACTTCACCGGAAACTTTGCTGAAATAGCTTTGCAATGCCTGCAAAGACTCCCAGCAACCAACACAAAATTCACTTACAACACCGATGGTCATACCTTCAACTACCTCGCCCATGATGGATTCAACTTACATGATCGTCATATTGCTATGGAATCTTTAGCTTACTGCGTTGTTGCTGTGGAATCTTTTGATCGCAATATTGCTATGGCCTTTCTTGATCGCATCAAGGAGGATTTTACCAAAAGATATGCTGGAGGAAAAGCTGCAACAGCAACATCTAAAAGCTTGAACAAAGAATTTGGACCGAAAATGAAGGAGCATATGCAATATTGTGTGGAACATCCAGAAGAGATTAGCAAACTTGCGAAAGTAAAAGCTCAGGTGTTTCAAGTTCTAGATGTTATGTTGGAAAATTTTGATCAGCTCAATAATCGTCAAGTGAAGATCGACGTTTTGATGGACAAAACTGACAACCTTCGTGATCAGGCACAAGTTTTCAGGGGGCAGGGAGGGCAATTAAGGAGAAAGATGTGA

Protein sequence

>MsG0780038625.01.T01

MAQQSLIYSFVARGTVILAEHTNFTGNFAEIALQCLQRLPATNTKFTYNTDGHTFNYLAHDGFNLHDRHIAMESLAYCVVAVESFDRNIAMAFLDRIKEDFTKRYAGGKAATATSKSLNKEFGPKMKEHMQYCVEHPEEISKLAKVKAQVFQVLDVMLENFDQLNNRQVKIDVLMDKTDNLRDQAQVFRGQGGQLRRKM*