Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041209.01.T01 | XP_013450239.1 | 83.077 | 130 | 5 | 2 | 1 | 113 | 1 | 130 | 4.22E-66 | 206 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041209.01.T01 | Q9FM54 | 55.556 | 126 | 38 | 2 | 2 | 109 | 4 | 129 | 2.18E-38 | 128 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041209.01.T01 | A0A072U3F9 | 83.077 | 130 | 5 | 2 | 1 | 113 | 1 | 130 | 2.02e-66 | 206 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048687.01 | MsG0780041209.01 | 0.854913 | 8.965897e-62 | 2.034012e-58 |
| MsG0180005337.01 | MsG0780041209.01 | 0.821616 | 3.672458e-53 | 3.002568e-50 |
| MsG0780040472.01 | MsG0780041209.01 | 0.805500 | 1.295578e-49 | 6.865106e-47 |
| MsG0780040926.01 | MsG0780041209.01 | 0.805801 | 1.120364e-49 | 5.982893e-47 |
| MsG0780041209.01 | MsG0880042544.01 | 0.809137 | 2.195058e-50 | 1.278532e-47 |
| MsG0780041209.01 | MsG0880046237.01 | 0.820862 | 5.481910e-53 | 4.389094e-50 |
| MsG0780041209.01 | MsG0880047388.01 | 0.834363 | 3.129328e-56 | 3.705476e-53 |
| MsG0380012073.01 | MsG0780041209.01 | 0.819947 | 8.884926e-53 | 6.935213e-50 |
| MsG0380013641.01 | MsG0780041209.01 | 0.804054 | 2.596784e-49 | 1.325836e-46 |
| MsG0380014778.01 | MsG0780041209.01 | 0.800008 | 1.762696e-48 | 8.118411e-46 |
| MsG0680030865.01 | MsG0780041209.01 | 0.842211 | 2.969106e-58 | 4.469557e-55 |
| MsG0680030968.01 | MsG0780041209.01 | 0.807003 | 6.250294e-50 | 3.442123e-47 |
| MsG0480020597.01 | MsG0780041209.01 | 0.804961 | 1.680125e-49 | 8.780914e-47 |
| MsG0480022960.01 | MsG0780041209.01 | 0.815747 | 7.889876e-52 | 5.486381e-49 |
| MsG0480023559.01 | MsG0780041209.01 | 0.808764 | 2.637723e-50 | 1.521054e-47 |
| MsG0580026059.01 | MsG0780041209.01 | 0.805174 | 1.516397e-49 | 7.968402e-47 |
| MsG0380016290.01 | MsG0780041209.01 | 0.811642 | 6.320336e-51 | 3.933262e-48 |
| MsG0380016389.01 | MsG0780041209.01 | 0.817571 | 3.078139e-52 | 2.249782e-49 |
| MsG0380017784.01 | MsG0780041209.01 | 0.814527 | 1.472656e-51 | 9.905435e-49 |
| MsG0580028060.01 | MsG0780041209.01 | 0.823211 | 1.565250e-53 | 1.339094e-50 |
| MsG0580028897.01 | MsG0780041209.01 | 0.801956 | 7.050053e-49 | 3.411219e-46 |
| MsG0280007196.01 | MsG0780041209.01 | 0.836895 | 7.156602e-57 | 9.143961e-54 |
| MsG0680035888.01 | MsG0780041209.01 | 0.824317 | 8.620400e-54 | 7.608538e-51 |
| MsG0780038400.01 | MsG0780041209.01 | 0.804695 | 1.909236e-49 | 9.909762e-47 |
| MsG0780038587.01 | MsG0780041209.01 | 0.801175 | 1.019312e-48 | 4.835716e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0780041209.01 | MsG0780041208.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041209.01.T01 | MTR_7g106600 | 83.077 | 130 | 5 | 2 | 1 | 113 | 1 | 130 | 5.12e-70 | 206 |
| MsG0780041209.01.T01 | MTR_2g088720 | 75.194 | 129 | 15 | 2 | 1 | 112 | 4 | 132 | 6.98e-64 | 190 |
| MsG0780041209.01.T01 | MTR_2g088720 | 75.194 | 129 | 15 | 2 | 1 | 112 | 15 | 143 | 1.14e-63 | 190 |
| MsG0780041209.01.T01 | MTR_7g106580 | 70.543 | 129 | 21 | 1 | 2 | 113 | 5 | 133 | 5.85e-58 | 175 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041209.01.T01 | AT5G62980 | 55.556 | 126 | 38 | 2 | 2 | 109 | 4 | 129 | 2.22e-39 | 128 |
| MsG0780041209.01.T01 | AT5G62980 | 55.556 | 126 | 38 | 2 | 2 | 109 | 4 | 129 | 2.22e-39 | 128 |
| MsG0780041209.01.T01 | AT3G11750 | 52.893 | 121 | 39 | 2 | 7 | 109 | 21 | 141 | 9.51e-36 | 119 |
| MsG0780041209.01.T01 | AT3G21730 | 43.333 | 120 | 50 | 3 | 8 | 109 | 40 | 159 | 2.71e-26 | 96.3 |
Find 25 sgRNAs with CRISPR-Local
Find 73 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTGAGGGGATTGACATTCTT+TGG | 0.182580 | 7:+88238521 | MsG0780041209.01.T01:CDS |
| AAAGCCTCATGTGGCTATTC+AGG | 0.228909 | 7:+88239563 | MsG0780041209.01.T01:CDS |
| TTATTTATTTATTTAATTGC+AGG | 0.332362 | 7:+88238456 | MsG0780041209.01.T01:five_prime_UTR |
| TTAGCTACGCTGAAATATAT+CGG | 0.447993 | 7:+88238657 | MsG0780041209.01.T01:CDS |
| TCAACGCCTAAGTAATCAAC+CGG | 0.493700 | 7:-88239588 | None:intergenic |
| TCATGTGGCTATTCAGGGTC+CGG | 0.501646 | 7:+88239569 | MsG0780041209.01.T01:CDS |
| AAGCCTCATGTGGCTATTCA+GGG | 0.505045 | 7:+88239564 | MsG0780041209.01.T01:CDS |
| AGAGAGAGATAACGAGTGTG+AGG | 0.506024 | 7:-88238375 | None:intergenic |
| TGGATGGATCTTAAACCAGC+TGG | 0.524717 | 7:+88238608 | MsG0780041209.01.T01:CDS |
| CAGGGTCCGGTTGATTACTT+AGG | 0.534205 | 7:+88239582 | MsG0780041209.01.T01:CDS |
| CTACAACGAACTTCTGTCCA+AGG | 0.552732 | 7:-88238574 | None:intergenic |
| CTCTTTGTGAATTGTCAAAG+TGG | 0.557952 | 7:-88239514 | None:intergenic |
| GAGAGAGATAACGAGTGTGA+GGG | 0.566524 | 7:-88238374 | None:intergenic |
| GTGGAGACAAACTGATTCTG+AGG | 0.567883 | 7:+88238504 | MsG0780041209.01.T01:CDS |
| TACAACGAACTTCTGTCCAA+GGG | 0.568116 | 7:-88238573 | None:intergenic |
| TCTGCTGTTCGTGTGAAGAT+CGG | 0.568597 | 7:+88239540 | MsG0780041209.01.T01:CDS |
| AAATGATCAGAAATTCCAGC+TGG | 0.574592 | 7:-88238623 | None:intergenic |
| AAAATGGATGCGTTAATAGG+TGG | 0.579943 | 7:+88238485 | MsG0780041209.01.T01:exon |
| TGTAGATATAGATGCTTGGA+TGG | 0.580239 | 7:+88238592 | MsG0780041209.01.T01:CDS |
| TGGAGACAAACTGATTCTGA+GGG | 0.582302 | 7:+88238505 | MsG0780041209.01.T01:CDS |
| GTGTGAGGGTGTGCATGTAA+TGG | 0.591719 | 7:-88238360 | None:intergenic |
| TCGTTGTAGATATAGATGCT+TGG | 0.592315 | 7:+88238588 | MsG0780041209.01.T01:CDS |
| GAAGATCGGAAAGCCTCATG+TGG | 0.680483 | 7:+88239554 | MsG0780041209.01.T01:CDS |
| GGACCCTGAATAGCCACATG+AGG | 0.705107 | 7:-88239567 | None:intergenic |
| GGAGACAAACTGATTCTGAG+GGG | 0.749444 | 7:+88238506 | MsG0780041209.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAAATTAAATTAAATAGTAG+TGG | - | Chr7:88238841-88238860 | None:intergenic | 10.0% |
| !! | TTATTTATTTATTTAATTGC+AGG | + | Chr7:88238456-88238475 | MsG0780041209.01.T01:five_prime_UTR | 10.0% |
| !! | AAATACAATTCAAAATAACA+GGG | - | Chr7:88239062-88239081 | None:intergenic | 15.0% |
| !! | TATACTGCTATAAATTATAA+TGG | + | Chr7:88239002-88239021 | MsG0780041209.01.T01:intron | 15.0% |
| !!! | AATCTCTGAAAAATAAATAA+CGG | + | Chr7:88238921-88238940 | MsG0780041209.01.T01:intron | 15.0% |
| !! | ATCTACAGTATAATTTGAAT+TGG | - | Chr7:88238898-88238917 | None:intergenic | 20.0% |
| !! | CTAAAACAGAAGATAAAATA+GGG | - | Chr7:88239298-88239317 | None:intergenic | 20.0% |
| !! | GAAATACAATTCAAAATAAC+AGG | - | Chr7:88239063-88239082 | None:intergenic | 20.0% |
| !! | TCATAAGAATAATGATAAAG+TGG | - | Chr7:88238696-88238715 | None:intergenic | 20.0% |
| !!! | TCAAAAATTACTCTTCTTTT+GGG | - | Chr7:88239360-88239379 | None:intergenic | 20.0% |
| !!! | TCTAAAACAGAAGATAAAAT+AGG | - | Chr7:88239299-88239318 | None:intergenic | 20.0% |
| ! | AAAAAAATGGATGCGTTAAT+AGG | + | Chr7:88238482-88238501 | MsG0780041209.01.T01:exon | 25.0% |
| ! | AAATTTAATATCATCAACCC+TGG | - | Chr7:88238968-88238987 | None:intergenic | 25.0% |
| ! | CTATCATTGAGAGTTTATAT+GGG | - | Chr7:88239235-88239254 | None:intergenic | 25.0% |
| ! | TAACATTCTGAACATACTTT+GGG | + | Chr7:88239108-88239127 | MsG0780041209.01.T01:intron | 25.0% |
| ! | TTAAACTAAACATACACACT+TGG | + | Chr7:88239412-88239431 | MsG0780041209.01.T01:intron | 25.0% |
| ! | TTAACATTCTGAACATACTT+TGG | + | Chr7:88239107-88239126 | MsG0780041209.01.T01:intron | 25.0% |
| !! | ATAAATAACGGAGTTGTTAA+TGG | + | Chr7:88238933-88238952 | MsG0780041209.01.T01:intron | 25.0% |
| !! | CAAAAGAAGAGTAATTTTTG+AGG | + | Chr7:88239359-88239378 | MsG0780041209.01.T01:intron | 25.0% |
| !! | CTCAAAAATTACTCTTCTTT+TGG | - | Chr7:88239361-88239380 | None:intergenic | 25.0% |
| !! | TGAATAGTTTGTGCTTAATT+TGG | + | Chr7:88239083-88239102 | MsG0780041209.01.T01:intron | 25.0% |
| !!! | TTATGAGTGAGTATTGAAAA+TGG | + | Chr7:88238792-88238811 | MsG0780041209.01.T01:intron | 25.0% |
| ATAAAGGAAGAAAAAACCCT+TGG | + | Chr7:88238557-88238576 | MsG0780041209.01.T01:CDS | 30.0% | |
| GCTATCATTGAGAGTTTATA+TGG | - | Chr7:88239236-88239255 | None:intergenic | 30.0% | |
| GTTTATATGGGAAATAAGCT+TGG | - | Chr7:88239223-88239242 | None:intergenic | 30.0% | |
| TAATTGCAGGAGCAAAAAAA+TGG | + | Chr7:88238469-88238488 | MsG0780041209.01.T01:exon | 30.0% | |
| TTAGCTACGCTGAAATATAT+CGG | + | Chr7:88238657-88238676 | MsG0780041209.01.T01:CDS | 30.0% | |
| ! | AAAGTGGAGATTGCAATTTT+TGG | - | Chr7:88239501-88239520 | None:intergenic | 30.0% |
| ! | AAGTGGAGATTGCAATTTTT+GGG | - | Chr7:88239500-88239519 | None:intergenic | 30.0% |
| ! | CAAGCATTGTGAAACTATTT+AGG | + | Chr7:88239169-88239188 | MsG0780041209.01.T01:intron | 30.0% |
| !!! | GAGTGAGTGTTGAATATTTT+TGG | + | Chr7:88238765-88238784 | MsG0780041209.01.T01:intron | 30.0% |
| !!! | TTGACATTCTTTGGTTTTCA+TGG | + | Chr7:88238530-88238549 | MsG0780041209.01.T01:CDS | 30.0% |
| AAAATGGATGCGTTAATAGG+TGG | + | Chr7:88238485-88238504 | MsG0780041209.01.T01:exon | 35.0% | |
| AAATGATCAGAAATTCCAGC+TGG | - | Chr7:88238626-88238645 | None:intergenic | 35.0% | |
| AAGCTCCACAAACATATGTT+TGG | - | Chr7:88239385-88239404 | None:intergenic | 35.0% | |
| ATAGCTAAGGAAGTTGTTGA+AGG | + | Chr7:88239445-88239464 | MsG0780041209.01.T01:intron | 35.0% | |
| CTCTTTGTGAATTGTCAAAG+TGG | - | Chr7:88239517-88239536 | None:intergenic | 35.0% | |
| GTTAATGGTAAATCTGTCCA+GGG | + | Chr7:88238948-88238967 | MsG0780041209.01.T01:intron | 35.0% | |
| TCACTCTGAGCATTAAGTTT+AGG | - | Chr7:88238751-88238770 | None:intergenic | 35.0% | |
| TCGTTGTAGATATAGATGCT+TGG | + | Chr7:88238588-88238607 | MsG0780041209.01.T01:CDS | 35.0% | |
| TGTAGATATAGATGCTTGGA+TGG | + | Chr7:88238592-88238611 | MsG0780041209.01.T01:CDS | 35.0% | |
| TGTTAATGGTAAATCTGTCC+AGG | + | Chr7:88238947-88238966 | MsG0780041209.01.T01:intron | 35.0% | |
| ! | GAACATACTTTGGGTTTGTT+TGG | + | Chr7:88239117-88239136 | MsG0780041209.01.T01:intron | 35.0% |
| ! | TGGTTTTCATGGAGCAATAA+AGG | + | Chr7:88238541-88238560 | MsG0780041209.01.T01:CDS | 35.0% |
| !! | TACTCTTCTTTTGGGAACAA+AGG | - | Chr7:88239352-88239371 | None:intergenic | 35.0% |
| CTCTCTCTCTCTACTACTTT+AGG | + | Chr7:88238420-88238439 | MsG0780041209.01.T01:five_prime_UTR | 40.0% | |
| TACAACGAACTTCTGTCCAA+GGG | - | Chr7:88238576-88238595 | None:intergenic | 40.0% | |
| TCAACGCCTAAGTAATCAAC+CGG | - | Chr7:88239591-88239610 | None:intergenic | 40.0% | |
| TGAGGCCAAACATATGTTTG+TGG | + | Chr7:88239377-88239396 | MsG0780041209.01.T01:intron | 40.0% | |
| ! | TGGAGACAAACTGATTCTGA+GGG | + | Chr7:88238505-88238524 | MsG0780041209.01.T01:CDS | 40.0% |
| ! | TGGTTGATGCAGTATAGCTA+AGG | + | Chr7:88239432-88239451 | MsG0780041209.01.T01:intron | 40.0% |
| !! | TCAAAACCTTCTGGAATCAG+TGG | + | Chr7:88239474-88239493 | MsG0780041209.01.T01:intron | 40.0% |
| AAAGCCTCATGTGGCTATTC+AGG | + | Chr7:88239563-88239582 | MsG0780041209.01.T01:CDS | 45.0% | |
| AAGCCTCATGTGGCTATTCA+GGG | + | Chr7:88239564-88239583 | MsG0780041209.01.T01:CDS | 45.0% | |
| AGAGAGAGATAACGAGTGTG+AGG | - | Chr7:88238378-88238397 | None:intergenic | 45.0% | |
| CTACAACGAACTTCTGTCCA+AGG | - | Chr7:88238577-88238596 | None:intergenic | 45.0% | |
| CTGAGGGGATTGACATTCTT+TGG | + | Chr7:88238521-88238540 | MsG0780041209.01.T01:CDS | 45.0% | |
| GAGAGAGATAACGAGTGTGA+GGG | - | Chr7:88238377-88238396 | None:intergenic | 45.0% | |
| GGCAGTACCAATGTTAGACT+CGG | - | Chr7:88239041-88239060 | None:intergenic | 45.0% | |
| TCTGCTGTTCGTGTGAAGAT+CGG | + | Chr7:88239540-88239559 | MsG0780041209.01.T01:CDS | 45.0% | |
| TGGATGGATCTTAAACCAGC+TGG | + | Chr7:88238608-88238627 | MsG0780041209.01.T01:CDS | 45.0% | |
| ! | GGAGACAAACTGATTCTGAG+GGG | + | Chr7:88238506-88238525 | MsG0780041209.01.T01:CDS | 45.0% |
| ! | GTGGAGACAAACTGATTCTG+AGG | + | Chr7:88238504-88238523 | MsG0780041209.01.T01:CDS | 45.0% |
| !!! | GATTCCAGAAGGTTTTGAGC+TGG | - | Chr7:88239472-88239491 | None:intergenic | 45.0% |
| AGGACCAGCTCAAAACCTTC+TGG | + | Chr7:88239465-88239484 | MsG0780041209.01.T01:intron | 50.0% | |
| CAGGGTCCGGTTGATTACTT+AGG | + | Chr7:88239582-88239601 | MsG0780041209.01.T01:CDS | 50.0% | |
| CCACGTACCGAGTCTAACAT+TGG | + | Chr7:88239031-88239050 | MsG0780041209.01.T01:intron | 50.0% | |
| GAAGATCGGAAAGCCTCATG+TGG | + | Chr7:88239554-88239573 | MsG0780041209.01.T01:CDS | 50.0% | |
| TCATGTGGCTATTCAGGGTC+CGG | + | Chr7:88239569-88239588 | MsG0780041209.01.T01:CDS | 50.0% | |
| ! | CCAATGTTAGACTCGGTACG+TGG | - | Chr7:88239034-88239053 | None:intergenic | 50.0% |
| ! | GTGTGAGGGTGTGCATGTAA+TGG | - | Chr7:88238363-88238382 | None:intergenic | 50.0% |
| !! | TTTGGGCCACTGATTCCAGA+AGG | - | Chr7:88239483-88239502 | None:intergenic | 50.0% |
| GGACCCTGAATAGCCACATG+AGG | - | Chr7:88239570-88239589 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 88238352 | 88239644 | 88238352 | ID=MsG0780041209.01;Name=MsG0780041209.01 |
| Chr7 | mRNA | 88238352 | 88239644 | 88238352 | ID=MsG0780041209.01.T01;Parent=MsG0780041209.01;Name=MsG0780041209.01.T01;_AED=0.45;_eAED=0.46;_QI=136|0|0.5|1|1|1|2|0|113 |
| Chr7 | exon | 88238352 | 88238678 | 88238352 | ID=MsG0780041209.01.T01:exon:3326;Parent=MsG0780041209.01.T01 |
| Chr7 | exon | 88239494 | 88239644 | 88239494 | ID=MsG0780041209.01.T01:exon:3327;Parent=MsG0780041209.01.T01 |
| Chr7 | five_prime_UTR | 88238352 | 88238487 | 88238352 | ID=MsG0780041209.01.T01:five_prime_utr;Parent=MsG0780041209.01.T01 |
| Chr7 | CDS | 88238488 | 88238678 | 88238488 | ID=MsG0780041209.01.T01:cds;Parent=MsG0780041209.01.T01 |
| Chr7 | CDS | 88239494 | 88239644 | 88239494 | ID=MsG0780041209.01.T01:cds;Parent=MsG0780041209.01.T01 |
| Gene Sequence |
| Protein sequence |
