AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880043201.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880043201.01.T01 MTR_8g007375 95.492 244 11 0 1 244 164 407 6.44e-175 487
MsG0880043201.01.T01 MTR_8g031510 97.126 174 5 0 71 244 1 174 8.86e-123 346
MsG0880043201.01.T01 MTR_2g025330 55.367 177 44 3 1 177 106 247 3.71e-57 183
MsG0880043201.01.T01 MTR_1g072300 33.190 232 150 2 4 235 262 488 9.52e-36 132
MsG0880043201.01.T01 MTR_8g069570 30.290 241 161 3 3 236 244 484 1.37e-31 121
MsG0880043201.01.T01 MTR_2g090290 30.841 214 138 3 4 209 254 465 1.55e-28 112
MsG0880043201.01.T01 MTR_1g086820 32.512 203 132 3 3 204 251 449 3.54e-28 111
MsG0880043201.01.T01 MTR_8g059115 30.364 247 158 4 2 235 163 408 7.37e-28 110
MsG0880043201.01.T01 MTR_3g099753 27.119 236 168 1 5 236 263 498 1.45e-27 110
MsG0880043201.01.T01 MTR_3g099753 27.119 236 168 1 5 236 287 522 1.81e-27 110
MsG0880043201.01.T01 MTR_8g465890 32.367 207 137 3 4 209 252 456 8.25e-26 105
MsG0880043201.01.T01 MTR_2g047855 31.308 214 143 4 4 215 253 464 3.36e-23 97.8
MsG0880043201.01.T01 MTR_2g013450 26.154 195 143 1 2 195 249 443 6.96e-17 79.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880043201.01.T01 AT1G02150 50.211 237 111 2 5 241 292 521 1.04e-79 249
MsG0880043201.01.T01 AT1G60770 30.928 194 132 1 3 194 244 437 1.69e-29 115
MsG0880043201.01.T01 AT5G27460 29.245 212 150 0 5 216 266 477 1.27e-28 113
MsG0880043201.01.T01 AT4G02820 30.732 205 140 1 4 208 282 484 1.38e-27 110
MsG0880043201.01.T01 AT2G20710 33.503 197 128 3 3 198 165 359 1.78e-26 106
MsG0880043201.01.T01 AT2G20710 33.503 197 128 3 3 198 260 454 4.55e-26 105
MsG0880043201.01.T01 AT4G21705 27.404 208 150 1 5 211 249 456 7.66e-22 94.0
MsG0880043201.01.T01 AT4G01990 26.699 206 150 1 3 208 261 465 7.02e-19 85.5
MsG0880043201.01.T01 AT1G28020 38.889 108 65 1 6 112 262 369 9.56e-16 76.6
MsG0880043201.01.T01 AT1G02370 33.010 103 69 0 3 105 293 395 2.26e-15 75.5
MsG0880043201.01.T01 AT1G80270 26.500 200 133 4 3 198 348 537 8.97e-12 64.7
MsG0880043201.01.T01 AT1G80270 26.500 200 133 4 3 198 348 537 8.97e-12 64.7
MsG0880043201.01.T01 AT1G80270 26.500 200 133 4 3 198 348 537 8.97e-12 64.7
MsG0880043201.01.T01 AT1G80270 26.500 200 133 4 3 198 348 537 8.97e-12 64.7
MsG0880043201.01.T01 AT1G80270 26.500 200 133 4 3 198 348 537 8.97e-12 64.7
MsG0880043201.01.T01 AT1G07590 26.000 200 144 2 3 199 286 484 3.79e-11 62.8

Find 53 sgRNAs with CRISPR-Local

Find 62 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCTCCCATGTAGTCAAATT+TGG 0.254937 8:-20514593 None:intergenic
TTCTGAGACGCTTTCGTGCT+TGG 0.330616 8:+20514649 MsG0880043201.01.T01:CDS
GAAACCCAAGCCAATAAAAT+TGG 0.333547 8:+20514706 MsG0880043201.01.T01:CDS
CTTCATCTTGACAAAGCTTA+AGG 0.339454 8:-20514736 None:intergenic
AAGACCGAGGGTAGAATATT+AGG 0.348676 8:+20514254 MsG0880043201.01.T01:CDS
CATTATCTCCTGAGTTTGTA+TGG 0.359485 8:+20514296 MsG0880043201.01.T01:CDS
TATTTCTTCTCTCGTAAGAA+TGG 0.377223 8:+20514409 MsG0880043201.01.T01:CDS
CCGCATATGCTTCATCGTTA+TGG 0.390851 8:-20514811 None:intergenic
CCCTCTGATGATTCTAGAAT+AGG 0.408766 8:+20514482 MsG0880043201.01.T01:CDS
CAGAAAAGCTTTACGAGGAA+TGG 0.415843 8:+20514447 MsG0880043201.01.T01:CDS
GTTTCCAAGTATACCTAACT+TGG 0.418407 8:+20514373 MsG0880043201.01.T01:CDS
ATTCTTTCAGTCCTGTTTGA+CGG 0.431698 8:-20514854 None:intergenic
ATAGCATGGTATCCCAAGTT+AGG 0.432336 8:-20514386 None:intergenic
ATAAGCTGGTATATTAAGAA+AGG 0.440983 8:+20514515 MsG0880043201.01.T01:CDS
ACGAGGAATGGGTTTCTGTA+AGG 0.443573 8:+20514459 MsG0880043201.01.T01:CDS
ATTGAGTTGTTGAGGAAGCC+AGG 0.457519 8:+20514785 MsG0880043201.01.T01:CDS
CGTAAGAATGGACGATATTG+AGG 0.465330 8:+20514421 MsG0880043201.01.T01:CDS
GCAAAGATGAACCGTCAAAC+AGG 0.466263 8:+20514843 MsG0880043201.01.T01:CDS
GCTTCATCGTTATGGTATCC+TGG 0.467840 8:-20514803 None:intergenic
TGACAAGTGATTCGAAGAGT+TGG 0.467988 8:+20514684 MsG0880043201.01.T01:CDS
AGAAAAGCTTTACGAGGAAT+GGG 0.471834 8:+20514448 MsG0880043201.01.T01:CDS
TATCCCAAGTTAGGTATACT+TGG 0.477102 8:-20514377 None:intergenic
TAGGAAACCTTCTCATAAGC+TGG 0.515507 8:+20514501 MsG0880043201.01.T01:CDS
TTGATTTATAGTTATTCCAC+AGG 0.522855 8:-20514349 None:intergenic
ATCAAAAGCTACTATCTACT+TGG 0.530755 8:-20514931 None:intergenic
TAAGCTGGTATATTAAGAAA+GGG 0.530989 8:+20514516 MsG0880043201.01.T01:CDS
CAACACTTCCATACAAACTC+AGG 0.539796 8:-20514304 None:intergenic
CTGAGTTTGTATGGAAGTGT+TGG 0.541520 8:+20514305 MsG0880043201.01.T01:CDS
TTCTTTAAGCATATGAGTGA+AGG 0.550405 8:+20514560 MsG0880043201.01.T01:CDS
AGGGTCATATTGCCGAAAAG+AGG 0.552374 8:+20514624 MsG0880043201.01.T01:CDS
GTAAGAATGGACGATATTGA+GGG 0.554710 8:+20514422 MsG0880043201.01.T01:CDS
GTATGGAAGTGTTGGAAACA+AGG 0.554809 8:+20514313 MsG0880043201.01.T01:CDS
AAACTCAGGAGATAATGAAA+AGG 0.565979 8:-20514290 None:intergenic
TCGACCTAATATTCTACCCT+CGG 0.570101 8:-20514258 None:intergenic
AGGACGAAGTTTACCGCCTG+TGG 0.571432 8:+20514333 MsG0880043201.01.T01:CDS
GATGTCCAAATTTGACTACA+TGG 0.575923 8:+20514588 MsG0880043201.01.T01:CDS
CAACAACTCAATTAAAACCT+CGG 0.580666 8:-20514774 None:intergenic
TAATATACCAGCTTATGAGA+AGG 0.582826 8:-20514508 None:intergenic
GGGTCATATTGCCGAAAAGA+GGG 0.583094 8:+20514625 MsG0880043201.01.T01:CDS
TTTCCAAGTATACCTAACTT+GGG 0.589423 8:+20514374 MsG0880043201.01.T01:CDS
TTTAAGCATATGAGTGAAGG+TGG 0.593303 8:+20514563 MsG0880043201.01.T01:CDS
TCCTATTCTAGAATCATCAG+AGG 0.618071 8:-20514483 None:intergenic
CGAGAGAAGAAATAATAGCA+TGG 0.622616 8:-20514400 None:intergenic
ATTTATAGTTATTCCACAGG+CGG 0.629230 8:-20514346 None:intergenic
TGATGTTGACAGTGAAAACA+TGG 0.629492 8:+20514880 MsG0880043201.01.T01:CDS
TTGTCAAGATGAAGACGACA+TGG 0.631429 8:+20514745 MsG0880043201.01.T01:CDS
CCTATTCTAGAATCATCAGA+GGG 0.647606 8:-20514482 None:intergenic
CCATAACGATGAAGCATATG+CGG 0.648692 8:+20514811 MsG0880043201.01.T01:CDS
AAGCATATGAGTGAAGGTGG+TGG 0.657423 8:+20514566 MsG0880043201.01.T01:CDS
ATGTCCAAATTTGACTACAT+GGG 0.659932 8:+20514589 MsG0880043201.01.T01:CDS
TAAGAATGGACGATATTGAG+GGG 0.688947 8:+20514423 MsG0880043201.01.T01:CDS
AAGAATGGACGATATTGAGG+GGG 0.691386 8:+20514424 MsG0880043201.01.T01:CDS
AGACGACATGGAGAGCGCCG+AGG 0.710154 8:+20514757 MsG0880043201.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! ATAAGCTGGTATATTAAGAA+AGG + Chr8:20514515-20514534 MsG0880043201.01.T01:CDS 25.0%
! TAAGCTGGTATATTAAGAAA+GGG + Chr8:20514516-20514535 MsG0880043201.01.T01:CDS 25.0%
! TTGATTTATAGTTATTCCAC+AGG - Chr8:20514352-20514371 None:intergenic 25.0%
AAACTCAGGAGATAATGAAA+AGG - Chr8:20514293-20514312 None:intergenic 30.0%
ATGTCCAAATTTGACTACAT+GGG + Chr8:20514589-20514608 MsG0880043201.01.T01:CDS 30.0%
ATTTATAGTTATTCCACAGG+CGG - Chr8:20514349-20514368 None:intergenic 30.0%
CAACAACTCAATTAAAACCT+CGG - Chr8:20514777-20514796 None:intergenic 30.0%
TAATATACCAGCTTATGAGA+AGG - Chr8:20514511-20514530 None:intergenic 30.0%
TATTTCTTCTCTCGTAAGAA+TGG + Chr8:20514409-20514428 MsG0880043201.01.T01:CDS 30.0%
TTCTTTAAGCATATGAGTGA+AGG + Chr8:20514560-20514579 MsG0880043201.01.T01:CDS 30.0%
TTTCCAAGTATACCTAACTT+GGG + Chr8:20514374-20514393 MsG0880043201.01.T01:CDS 30.0%
!!! AGGTTTTAATTGAGTTGTTG+AGG + Chr8:20514777-20514796 MsG0880043201.01.T01:CDS 30.0%
AGAAAAGCTTTACGAGGAAT+GGG + Chr8:20514448-20514467 MsG0880043201.01.T01:CDS 35.0%
ATTCTTTCAGTCCTGTTTGA+CGG - Chr8:20514857-20514876 None:intergenic 35.0%
CATTATCTCCTGAGTTTGTA+TGG + Chr8:20514296-20514315 MsG0880043201.01.T01:CDS 35.0%
CGAGAGAAGAAATAATAGCA+TGG - Chr8:20514403-20514422 None:intergenic 35.0%
CTTCATCTTGACAAAGCTTA+AGG - Chr8:20514739-20514758 None:intergenic 35.0%
GAAACCCAAGCCAATAAAAT+TGG + Chr8:20514706-20514725 MsG0880043201.01.T01:CDS 35.0%
GATGTCCAAATTTGACTACA+TGG + Chr8:20514588-20514607 MsG0880043201.01.T01:CDS 35.0%
GTAAGAATGGACGATATTGA+GGG + Chr8:20514422-20514441 MsG0880043201.01.T01:CDS 35.0%
GTTTCCAAGTATACCTAACT+TGG + Chr8:20514373-20514392 MsG0880043201.01.T01:CDS 35.0%
TAAGAATGGACGATATTGAG+GGG + Chr8:20514423-20514442 MsG0880043201.01.T01:CDS 35.0%
TATCCCAAGTTAGGTATACT+TGG - Chr8:20514380-20514399 None:intergenic 35.0%
TGATGTTGACAGTGAAAACA+TGG + Chr8:20514880-20514899 MsG0880043201.01.T01:CDS 35.0%
TTTAAGCATATGAGTGAAGG+TGG + Chr8:20514563-20514582 MsG0880043201.01.T01:CDS 35.0%
! AGGAATGCAGCCAATTTTAT+TGG - Chr8:20514719-20514738 None:intergenic 35.0%
!! CCTATTCTAGAATCATCAGA+GGG - Chr8:20514485-20514504 None:intergenic 35.0%
!! TCCTATTCTAGAATCATCAG+AGG - Chr8:20514486-20514505 None:intergenic 35.0%
AAGAATGGACGATATTGAGG+GGG + Chr8:20514424-20514443 MsG0880043201.01.T01:CDS 40.0%
AAGACCGAGGGTAGAATATT+AGG + Chr8:20514254-20514273 MsG0880043201.01.T01:CDS 40.0%
AGCTCCCATGTAGTCAAATT+TGG - Chr8:20514596-20514615 None:intergenic 40.0%
ATAGCATGGTATCCCAAGTT+AGG - Chr8:20514389-20514408 None:intergenic 40.0%
CAACACTTCCATACAAACTC+AGG - Chr8:20514307-20514326 None:intergenic 40.0%
CAGAAAAGCTTTACGAGGAA+TGG + Chr8:20514447-20514466 MsG0880043201.01.T01:CDS 40.0%
CCATAACGATGAAGCATATG+CGG + Chr8:20514811-20514830 MsG0880043201.01.T01:CDS 40.0%
CGTAAGAATGGACGATATTG+AGG + Chr8:20514421-20514440 MsG0880043201.01.T01:CDS 40.0%
GAGTGCTTAAAAAAGACCGA+GGG + Chr8:20514242-20514261 MsG0880043201.01.T01:CDS 40.0%
TAGGAAACCTTCTCATAAGC+TGG + Chr8:20514501-20514520 MsG0880043201.01.T01:CDS 40.0%
TCGACCTAATATTCTACCCT+CGG - Chr8:20514261-20514280 None:intergenic 40.0%
TGACAAGTGATTCGAAGAGT+TGG + Chr8:20514684-20514703 MsG0880043201.01.T01:CDS 40.0%
TTGTCAAGATGAAGACGACA+TGG + Chr8:20514745-20514764 MsG0880043201.01.T01:CDS 40.0%
! ACATGGGAGCTTCTTTTTGA+GGG + Chr8:20514605-20514624 MsG0880043201.01.T01:CDS 40.0%
! AGAGTGCTTAAAAAAGACCG+AGG + Chr8:20514241-20514260 MsG0880043201.01.T01:CDS 40.0%
! CTGAGTTTGTATGGAAGTGT+TGG + Chr8:20514305-20514324 MsG0880043201.01.T01:CDS 40.0%
! TACATGGGAGCTTCTTTTTG+AGG + Chr8:20514604-20514623 MsG0880043201.01.T01:CDS 40.0%
!! CCCTCTGATGATTCTAGAAT+AGG + Chr8:20514482-20514501 MsG0880043201.01.T01:CDS 40.0%
!! GTATGGAAGTGTTGGAAACA+AGG + Chr8:20514313-20514332 MsG0880043201.01.T01:CDS 40.0%
!! TGCAGCCAATTTTATTGGCT+TGG - Chr8:20514714-20514733 None:intergenic 40.0%
!!! GCAGCCAATTTTATTGGCTT+GGG - Chr8:20514713-20514732 None:intergenic 40.0%
AAGCATATGAGTGAAGGTGG+TGG + Chr8:20514566-20514585 MsG0880043201.01.T01:CDS 45.0%
AGGGTCATATTGCCGAAAAG+AGG + Chr8:20514624-20514643 MsG0880043201.01.T01:CDS 45.0%
CCGCATATGCTTCATCGTTA+TGG - Chr8:20514814-20514833 None:intergenic 45.0%
GCAAAGATGAACCGTCAAAC+AGG + Chr8:20514843-20514862 MsG0880043201.01.T01:CDS 45.0%
GCTTCATCGTTATGGTATCC+TGG - Chr8:20514806-20514825 None:intergenic 45.0%
GGGTCATATTGCCGAAAAGA+GGG + Chr8:20514625-20514644 MsG0880043201.01.T01:CDS 45.0%
! ACGAGGAATGGGTTTCTGTA+AGG + Chr8:20514459-20514478 MsG0880043201.01.T01:CDS 45.0%
! ATTGAGTTGTTGAGGAAGCC+AGG + Chr8:20514785-20514804 MsG0880043201.01.T01:CDS 45.0%
! CGTCTCAGAAACCCTCTTTT+CGG - Chr8:20514639-20514658 None:intergenic 45.0%
!! TTCTGAGACGCTTTCGTGCT+TGG + Chr8:20514649-20514668 MsG0880043201.01.T01:CDS 50.0%
AGGACGAAGTTTACCGCCTG+TGG + Chr8:20514333-20514352 MsG0880043201.01.T01:CDS 55.0%
GGGGGCAGAAAAGCTTTACG+AGG + Chr8:20514442-20514461 MsG0880043201.01.T01:CDS 55.0%
!! AGACGACATGGAGAGCGCCG+AGG + Chr8:20514757-20514776 MsG0880043201.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr8 gene 20514218 20514952 20514218 ID=MsG0880043201.01;Name=MsG0880043201.01
Chr8 mRNA 20514218 20514952 20514218 ID=MsG0880043201.01.T01;Parent=MsG0880043201.01;Name=MsG0880043201.01.T01;_AED=0.22;_eAED=0.22;_QI=0|-1|0|1|-1|1|1|0|244
Chr8 exon 20514218 20514952 20514218 ID=MsG0880043201.01.T01:exon:11794;Parent=MsG0880043201.01.T01
Chr8 CDS 20514218 20514952 20514218 ID=MsG0880043201.01.T01:cds;Parent=MsG0880043201.01.T01
Gene Sequence

>MsG0880043201.01.T01

ATGGAACTGTTTGAAAAAGCACAAGAGTGCTTAAAAAAGACCGAGGGTAGAATATTAGGTCGAGATAAAGTACCTTTTCATTATCTCCTGAGTTTGTATGGAAGTGTTGGAAACAAGGACGAAGTTTACCGCCTGTGGAATAACTATAAATCAATGTTTCCAAGTATACCTAACTTGGGATACCATGCTATTATTTCTTCTCTCGTAAGAATGGACGATATTGAGGGGGCAGAAAAGCTTTACGAGGAATGGGTTTCTGTAAGGCCCTCTGATGATTCTAGAATAGGAAACCTTCTCATAAGCTGGTATATTAAGAAAGGGAAATCAGATAAAGTTTTTAGCTTCTTTAAGCATATGAGTGAAGGTGGTGGATGTCCAAATTTGACTACATGGGAGCTTCTTTTTGAGGGTCATATTGCCGAAAAGAGGGTTTCTGAGACGCTTTCGTGCTTGGAAAAAGCTTTTATGACAAGTGATTCGAAGAGTTGGAAACCCAAGCCAATAAAATTGGCTGCATTCCTTAAGCTTTGTCAAGATGAAGACGACATGGAGAGCGCCGAGGTTTTAATTGAGTTGTTGAGGAAGCCAGGATACCATAACGATGAAGCATATGCGGCACTCATTAGCAAAGATGAACCGTCAAACAGGACTGAAAGAATTGATGATGTTGACAGTGAAAACATGGATGATGATGACTCTCAAGTGTTGTTCAGCCAAGTAGATAGTAGCTTTTGA

Protein sequence

>MsG0880043201.01.T01

MELFEKAQECLKKTEGRILGRDKVPFHYLLSLYGSVGNKDEVYRLWNNYKSMFPSIPNLGYHAIISSLVRMDDIEGAEKLYEEWVSVRPSDDSRIGNLLISWYIKKGKSDKVFSFFKHMSEGGGCPNLTTWELLFEGHIAEKRVSETLSCLEKAFMTSDSKSWKPKPIKLAAFLKLCQDEDDMESAEVLIELLRKPGYHNDEAYAALISKDEPSNRTERIDDVDSENMDDDDSQVLFSQVDSSF*