AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780036659.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780036659.01.T01 MTR_7g016670 96.154 78 3 0 4 81 247 324 1.67e-48 155
MsG0780036659.01.T01 MTR_5g012850 80.000 80 16 0 2 81 270 349 4.90e-41 137
MsG0780036659.01.T01 MTR_5g012850 74.286 35 9 0 2 36 270 304 8.65e-12 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780036659.01.T01 AT4G17360 83.333 78 13 0 4 81 251 328 1.82e-42 140
MsG0780036659.01.T01 AT5G47435 83.333 78 13 0 4 81 246 323 2.16e-42 140
MsG0780036659.01.T01 AT5G47435 83.333 78 13 0 4 81 246 323 2.16e-42 140

Find 27 sgRNAs with CRISPR-Local

Find 110 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACATAATGTACACAAATATT+TGG 0.127201 7:-11477915 MsG0780036659.01.T01:three_prime_UTR
TTCACCATTTGTTCAATTAT+TGG 0.177833 7:+11479196 None:intergenic
CATAATGTACACAAATATTT+GGG 0.260083 7:-11477914 MsG0780036659.01.T01:three_prime_UTR
GAGGCTGGTGTGAAATTAAT+TGG 0.276061 7:-11479259 MsG0780036659.01.T01:CDS
TTGTTGCAGCTAACATAAAA+TGG 0.277800 7:+11477842 None:intergenic
ATAACTTAACTTCATTCGTT+TGG 0.370328 7:-11477808 MsG0780036659.01.T01:three_prime_UTR
ATATTTGTGTACATTATGTT+TGG 0.372293 7:+11477919 None:intergenic
TATCGTAAGGGCATTGGATG+TGG 0.381151 7:-11477781 MsG0780036659.01.T01:three_prime_UTR
ACAGTCTTCTTTACCTCATA+AGG 0.386943 7:+11477968 None:intergenic
TAGATCCTATTGTGAACTTC+GGG 0.387384 7:-11477996 MsG0780036659.01.T01:CDS
GGTGACCTGGGTTCGAACTA+TGG 0.391286 7:+11477755 None:intergenic
TTCGTTTGGTTCTTATCGTA+AGG 0.394008 7:-11477794 MsG0780036659.01.T01:three_prime_UTR
CGGACCAATAATTGAACAAA+TGG 0.444593 7:-11479200 MsG0780036659.01.T01:intron
TAATACCCGAAGTTCACAAT+AGG 0.456580 7:+11477991 None:intergenic
ATCGTAAGGGCATTGGATGT+GGG 0.457308 7:-11477780 MsG0780036659.01.T01:three_prime_UTR
ACTTCGGGTATTACCTTATG+AGG 0.458749 7:-11477981 MsG0780036659.01.T01:CDS
GGTTCTTATCGTAAGGGCAT+TGG 0.467165 7:-11477787 MsG0780036659.01.T01:three_prime_UTR
ACAATAGGATCTAATAGCCT+TGG 0.491868 7:+11478006 None:intergenic
TTAGATCCTATTGTGAACTT+CGG 0.497060 7:-11477997 MsG0780036659.01.T01:CDS
TGGAAAGACATTGTTTCTCA+AGG 0.521459 7:+11478026 None:intergenic
TCGTTTGGTTCTTATCGTAA+GGG 0.532073 7:-11477793 MsG0780036659.01.T01:three_prime_UTR
TGAGAAACAATGTCTTTCCA+AGG 0.572364 7:-11478023 MsG0780036659.01.T01:CDS
AAAGGGAAATGTGGTGACCT+GGG 0.582465 7:+11477743 None:intergenic
TCGTAAGGGCATTGGATGTG+GGG 0.595015 7:-11477779 MsG0780036659.01.T01:three_prime_UTR
ATAATGTACACAAATATTTG+GGG 0.667804 7:-11477913 MsG0780036659.01.T01:three_prime_UTR
GGGGTCCATAGTTCGAACCC+AGG 0.689018 7:-11477760 MsG0780036659.01.T01:three_prime_UTR
GTGAGCGAAGAACTTGACAG+CGG 0.735138 7:-11479220 MsG0780036659.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGATATTATATTATTTAAAG+TGG - Chr7:11477970-11477989 MsG0780036659.01.T01:CDS 10.0%
!!! ATTTTTCTTAAGCAAAAATT+GGG - Chr7:11477941-11477960 MsG0780036659.01.T01:exon 15.0%
!! ACATAATGTACACAAATATT+TGG - Chr7:11479120-11479139 MsG0780036659.01.T01:intron 20.0%
!! ATAATGTACACAAATATTTG+GGG - Chr7:11479122-11479141 MsG0780036659.01.T01:intron 20.0%
!! ATATTTGTGTACATTATGTT+TGG + Chr7:11479119-11479138 None:intergenic 20.0%
!! CATAATGTACACAAATATTT+GGG - Chr7:11479121-11479140 MsG0780036659.01.T01:intron 20.0%
!!! GATTTTTCTTAAGCAAAAAT+TGG - Chr7:11477940-11477959 MsG0780036659.01.T01:exon 20.0%
!!! TAGGACTCAAAATATATTTT+TGG + Chr7:11478175-11478194 None:intergenic 20.0%
! ATAACTTAACTTCATTCGTT+TGG - Chr7:11479227-11479246 MsG0780036659.01.T01:CDS 25.0%
! CATACTATCTTAATCTGTAT+AGG + Chr7:11478343-11478362 None:intergenic 25.0%
! CTATAGCTTATAAGATATCA+TGG + Chr7:11478750-11478769 None:intergenic 25.0%
! TATATATTCCTAGTATGCTA+AGG + Chr7:11478094-11478113 None:intergenic 25.0%
! TTCACCATTTGTTCAATTAT+TGG + Chr7:11477842-11477861 None:intergenic 25.0%
!! ATTTTTCTATACATGCTTCA+TGG - Chr7:11477867-11477886 MsG0780036659.01.T01:three_prime_UTR 25.0%
AACATTATCAATGTCCTGAA+GGG + Chr7:11478043-11478062 None:intergenic 30.0%
ATCAAGCATTTGAACAATAC+AGG + Chr7:11478636-11478655 None:intergenic 30.0%
ATCTTACAATTGTCTCAAAG+GGG - Chr7:11478004-11478023 MsG0780036659.01.T01:CDS 30.0%
CATCTTACAATTGTCTCAAA+GGG - Chr7:11478003-11478022 MsG0780036659.01.T01:CDS 30.0%
CTGTCAAATAGTCTAAATTG+TGG - Chr7:11478883-11478902 MsG0780036659.01.T01:intron 30.0%
CTTAAGCAAAAATTGGGAAA+AGG - Chr7:11477947-11477966 MsG0780036659.01.T01:exon 30.0%
CTTGATATATCCTATTCGTA+GGG - Chr7:11478650-11478669 MsG0780036659.01.T01:intron 30.0%
GATATATACCTTAGCATACT+AGG - Chr7:11478083-11478102 MsG0780036659.01.T01:CDS 30.0%
GCTTGATACTATTTATGTGA+GGG - Chr7:11478905-11478924 MsG0780036659.01.T01:intron 30.0%
TTAGATCCTATTGTGAACTT+CGG - Chr7:11479038-11479057 MsG0780036659.01.T01:intron 30.0%
TTGTTGCAGCTAACATAAAA+TGG + Chr7:11479196-11479215 None:intergenic 30.0%
!! GAAGACTGTTGTATTTTAAG+TGG - Chr7:11479081-11479100 MsG0780036659.01.T01:intron 30.0%
ACAATAGGATCTAATAGCCT+TGG + Chr7:11479032-11479051 None:intergenic 35.0%
ACAGTCTTCTTTACCTCATA+AGG + Chr7:11479070-11479089 None:intergenic 35.0%
ACATTATCAATGTCCTGAAG+GGG + Chr7:11478042-11478061 None:intergenic 35.0%
AGAAGAACCTATGCATACTA+GGG + Chr7:11478388-11478407 None:intergenic 35.0%
CAACATTATCAATGTCCTGA+AGG + Chr7:11478044-11478063 None:intergenic 35.0%
CGGACCAATAATTGAACAAA+TGG - Chr7:11477835-11477854 MsG0780036659.01.T01:three_prime_UTR 35.0%
CTATTTATGTGAGGGTAACA+TGG - Chr7:11478913-11478932 MsG0780036659.01.T01:intron 35.0%
GCATCTTACAATTGTCTCAA+AGG - Chr7:11478002-11478021 MsG0780036659.01.T01:CDS 35.0%
GCTTGATATATCCTATTCGT+AGG - Chr7:11478649-11478668 MsG0780036659.01.T01:intron 35.0%
GGCTTGATACTATTTATGTG+AGG - Chr7:11478904-11478923 MsG0780036659.01.T01:intron 35.0%
TAATACCCGAAGTTCACAAT+AGG + Chr7:11479047-11479066 None:intergenic 35.0%
TAGATCCTATTGTGAACTTC+GGG - Chr7:11479039-11479058 MsG0780036659.01.T01:intron 35.0%
TCATTCACATCTTATTGCCA+AGG + Chr7:11477913-11477932 None:intergenic 35.0%
TGAGAAACAATGTCTTTCCA+AGG - Chr7:11479012-11479031 MsG0780036659.01.T01:intron 35.0%
TGTTGATCTGAATTGAGCAT+AGG - Chr7:11478059-11478078 MsG0780036659.01.T01:CDS 35.0%
! TGGAAAGACATTGTTTCTCA+AGG + Chr7:11479012-11479031 None:intergenic 35.0%
! TTATATATGTGGGTTGCATG+AGG - Chr7:11478469-11478488 MsG0780036659.01.T01:intron 35.0%
!! ACTTTTGTTTGATGTGTTGC+TGG - Chr7:11478589-11478608 MsG0780036659.01.T01:intron 35.0%
!! TCGTTTGGTTCTTATCGTAA+GGG - Chr7:11479242-11479261 MsG0780036659.01.T01:CDS 35.0%
!! TTCGTTTGGTTCTTATCGTA+AGG - Chr7:11479241-11479260 MsG0780036659.01.T01:CDS 35.0%
!!! ATATTTTTGGATGCTTGCAC+TGG + Chr7:11478162-11478181 None:intergenic 35.0%
ACCCACATATATAAGTCGCT+AGG + Chr7:11478463-11478482 None:intergenic 40.0%
ACTTCGGGTATTACCTTATG+AGG - Chr7:11479054-11479073 MsG0780036659.01.T01:intron 40.0%
ATCTTATTGCCAAGGAAGCA+AGG + Chr7:11477905-11477924 None:intergenic 40.0%
CAAGGGATAATGCAAATGAG+AGG - Chr7:11478492-11478511 MsG0780036659.01.T01:intron 40.0%
CACATATATAAGTCGCTAGG+CGG + Chr7:11478460-11478479 None:intergenic 40.0%
GAGAAGAACCTATGCATACT+AGG + Chr7:11478389-11478408 None:intergenic 40.0%
GAGGCTGGTGTGAAATTAAT+TGG - Chr7:11477776-11477795 MsG0780036659.01.T01:three_prime_UTR 40.0%
GCCTAGCGACTTATATATGT+GGG - Chr7:11478459-11478478 MsG0780036659.01.T01:intron 40.0%
AGAGGAACCTGTGCATACTA+GGG - Chr7:11478510-11478529 MsG0780036659.01.T01:intron 45.0%
CAATCGAACCTAAGACCTTG+AGG + Chr7:11478255-11478274 None:intergenic 45.0%
CATGGCTGAATCTCATTTGC+AGG - Chr7:11478931-11478950 MsG0780036659.01.T01:intron 45.0%
CCTATTCGTAGGGTCTGTTT+GGG - Chr7:11478660-11478679 MsG0780036659.01.T01:intron 45.0%
CGCCTAGCGACTTATATATG+TGG - Chr7:11478458-11478477 MsG0780036659.01.T01:intron 45.0%
CTAGGGATGGTGCAATTAAG+AGG + Chr7:11478371-11478390 None:intergenic 45.0%
CTCACATTTCCTTGCTTCCT+TGG - Chr7:11477893-11477912 MsG0780036659.01.T01:three_prime_UTR 45.0%
GAACCTATGCATACTAGGGA+TGG + Chr7:11478384-11478403 None:intergenic 45.0%
GAGTCCTATAGAACCCACTT+GGG - Chr7:11478187-11478206 MsG0780036659.01.T01:intron 45.0%
GTCTTAGGTTCGATTGTCTC+CGG - Chr7:11478259-11478278 MsG0780036659.01.T01:intron 45.0%
TAAACTAGCCCCACCCAAAT+TGG + Chr7:11478287-11478306 None:intergenic 45.0%
TCCTATTCGTAGGGTCTGTT+TGG - Chr7:11478659-11478678 MsG0780036659.01.T01:intron 45.0%
TGAGTCCTATAGAACCCACT+TGG - Chr7:11478186-11478205 MsG0780036659.01.T01:intron 45.0%
TTATCAATGTCCTGAAGGGG+AGG + Chr7:11478039-11478058 None:intergenic 45.0%
! ATTGTCTCCGGTGCCAATTT+GGG - Chr7:11478271-11478290 MsG0780036659.01.T01:intron 45.0%
! GGTTCTTATCGTAAGGGCAT+TGG - Chr7:11479248-11479267 MsG0780036659.01.T01:CDS 45.0%
!! ATCGTAAGGGCATTGGATGT+GGG - Chr7:11479255-11479274 MsG0780036659.01.T01:CDS 45.0%
!! TATCGTAAGGGCATTGGATG+TGG - Chr7:11479254-11479273 MsG0780036659.01.T01:CDS 45.0%
AGGCGGTTGCATGTCAACTA+TGG + Chr7:11478443-11478462 None:intergenic 50.0%
CCCAAACAGACCCTACGAAT+AGG + Chr7:11478663-11478682 None:intergenic 50.0%
CGGTTGCATGTCAACTATGG+TGG + Chr7:11478440-11478459 None:intergenic 50.0%
CTAGGGGTGGTGCAATTAAG+AGG - Chr7:11478527-11478546 MsG0780036659.01.T01:intron 50.0%
GAGAGGAACCTGTGCATACT+AGG - Chr7:11478509-11478528 MsG0780036659.01.T01:intron 50.0%
GAGGAACCTGTGCATACTAG+GGG - Chr7:11478511-11478530 MsG0780036659.01.T01:intron 50.0%
GATTTGTACTCCTCCCCTTC+AGG - Chr7:11478026-11478045 MsG0780036659.01.T01:CDS 50.0%
GCACCATCCCTAGTATGCAT+AGG - Chr7:11478378-11478397 MsG0780036659.01.T01:intron 50.0%
GTGAGCGAAGAACTTGACAG+CGG - Chr7:11477815-11477834 MsG0780036659.01.T01:three_prime_UTR 50.0%
GTGCTCTTCCTCAAGGTCTT+AGG - Chr7:11478244-11478263 MsG0780036659.01.T01:intron 50.0%
TTGGAGTGTGCTCTTCCTCA+AGG - Chr7:11478237-11478256 MsG0780036659.01.T01:intron 50.0%
! ATGGTGGCTTGCATGAGGTA+AGG + Chr7:11478424-11478443 None:intergenic 50.0%
! ATGTGGGTTGCATGAGGCAA+GGG - Chr7:11478475-11478494 MsG0780036659.01.T01:intron 50.0%
! CAACTATGGTGGCTTGCATG+AGG + Chr7:11478429-11478448 None:intergenic 50.0%
! CGAACCCAAGTGGGTTCTAT+AGG + Chr7:11478194-11478213 None:intergenic 50.0%
! GATTGTCTCCGGTGCCAATT+TGG - Chr7:11478270-11478289 MsG0780036659.01.T01:intron 50.0%
! TATGTGGGTTGCATGAGGCA+AGG - Chr7:11478474-11478493 MsG0780036659.01.T01:intron 50.0%
! TGGTGGCTTGCATGAGGTAA+GGG + Chr7:11478423-11478442 None:intergenic 50.0%
! TTGAGGAAGAGCACACTCCA+AGG + Chr7:11478238-11478257 None:intergenic 50.0%
!! TCGTAAGGGCATTGGATGTG+GGG - Chr7:11479256-11479275 MsG0780036659.01.T01:CDS 50.0%
!!! TTTTGGCAGGCTTTTGAGGC+TGG - Chr7:11477761-11477780 MsG0780036659.01.T01:three_prime_UTR 50.0%
GAACCTGTGCATACTAGGGG+TGG - Chr7:11478514-11478533 MsG0780036659.01.T01:intron 55.0%
! TCTCCGGTGCCAATTTGGGT+GGG - Chr7:11478275-11478294 MsG0780036659.01.T01:intron 55.0%
ACCACCAGGCGAACCCAAGT+GGG + Chr7:11478203-11478222 None:intergenic 60.0%
AGAACCCACTTGGGTTCGCC+TGG - Chr7:11478196-11478215 MsG0780036659.01.T01:intron 60.0%
AGCCCCACCCAAATTGGCAC+CGG + Chr7:11478281-11478300 None:intergenic 60.0%
GCACCACCCCTAGTATGCAC+AGG + Chr7:11478520-11478539 None:intergenic 60.0%
GGGGTCCATAGTTCGAACCC+AGG - Chr7:11479275-11479294 MsG0780036659.01.T01:CDS 60.0%
! CTCCGGTGCCAATTTGGGTG+GGG - Chr7:11478276-11478295 MsG0780036659.01.T01:intron 60.0%
! GTCTCCGGTGCCAATTTGGG+TGG - Chr7:11478274-11478293 MsG0780036659.01.T01:intron 60.0%
! TTGGGTTCGCCTGGTGGTGT+TGG - Chr7:11478205-11478224 MsG0780036659.01.T01:intron 60.0%
!! GTGGTGTTGGCTTGGGACCT+TGG - Chr7:11478218-11478237 MsG0780036659.01.T01:intron 60.0%
!! TCGCCTGGTGGTGTTGGCTT+GGG - Chr7:11478211-11478230 MsG0780036659.01.T01:intron 60.0%
!! TTCGCCTGGTGGTGTTGGCT+TGG - Chr7:11478210-11478229 MsG0780036659.01.T01:intron 60.0%
ACCCACTTGGGTTCGCCTGG+TGG - Chr7:11478199-11478218 MsG0780036659.01.T01:intron 65.0%
CACCACCAGGCGAACCCAAG+TGG + Chr7:11478204-11478223 None:intergenic 65.0%
GGTCCCAAGCCAACACCACC+AGG + Chr7:11478217-11478236 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr7 gene 11477758 11479299 11477758 ID=MsG0780036659.01;Name=MsG0780036659.01
Chr7 mRNA 11477758 11479299 11477758 ID=MsG0780036659.01.T01;Parent=MsG0780036659.01;Name=MsG0780036659.01.T01;_AED=0.46;_eAED=0.47;_QI=0|1|0.5|1|1|1|2|200|81
Chr7 exon 11477758 11478104 11477758 ID=MsG0780036659.01.T01:exon:1796;Parent=MsG0780036659.01.T01
Chr7 exon 11479201 11479299 11479201 ID=MsG0780036659.01.T01:exon:1795;Parent=MsG0780036659.01.T01
Chr7 CDS 11479201 11479299 11479201 ID=MsG0780036659.01.T01:cds;Parent=MsG0780036659.01.T01
Chr7 CDS 11477958 11478104 11477958 ID=MsG0780036659.01.T01:cds;Parent=MsG0780036659.01.T01
Chr7 three_prime_UTR 11477758 11477957 11477758 ID=MsG0780036659.01.T01:three_prime_utr;Parent=MsG0780036659.01.T01
Gene Sequence

>MsG0780036659.01.T01

TTGTTTTGGCAGGCTTTTGAGGCTGGTGTGAAATTAATTGGCGCGACAAGTCACTTTGTGAGCGAAGAACTTGACAGCGGACCAATAATTGAACAAATGGTTGAGAGAGTTTCACACAGAGATAACTTGCAGAGCTTTGTGCAGAAATCACAAAACCTTGAGAAACAATGTCTTTCCAAGGCTATTAGATCCTATTGTGAACTTCGGGTATTACCTTATGAGGTAAAGAAGACTGTTGTATTTTAA

Protein sequence

>MsG0780036659.01.T01

LFWQAFEAGVKLIGATSHFVSEELDSGPIIEQMVERVSHRDNLQSFVQKSQNLEKQCLSKAIRSYCELRVLPYEVKKTVVF*