AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880043313.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880043313.01.T01 MTR_0002s0040 55.822 292 97 9 1 280 1 272 4.62e-95 283
MsG0880043313.01.T01 MTR_0263s0020 56.507 292 95 9 1 280 1 272 1.58e-94 281
MsG0880043313.01.T01 MTR_1457s0010 66.376 229 53 8 65 289 1 209 1.92e-92 273
MsG0880043313.01.T01 MTR_0002s1060 51.546 291 108 11 4 280 6 277 4.68e-75 232
MsG0880043313.01.T01 MTR_0062s0020 38.542 288 140 8 14 280 4 275 1.63e-48 164
MsG0880043313.01.T01 MTR_0062s0090 39.510 286 138 10 14 280 4 273 9.68e-48 161
MsG0880043313.01.T01 MTR_0062s0040 39.510 286 138 10 14 280 4 273 9.68e-48 161
MsG0880043313.01.T01 MTR_0062s0060 39.510 286 138 10 14 280 4 273 9.68e-48 161
MsG0880043313.01.T01 MTR_0443s0040 39.175 291 132 12 14 280 16 285 1.83e-47 161
MsG0880043313.01.T01 MTR_0062s0170 36.304 303 151 10 1 280 1 284 9.25e-39 139
MsG0880043313.01.T01 MTR_0062s0110 36.304 303 151 10 1 280 1 284 9.25e-39 139
MsG0880043313.01.T01 MTR_0062s0130 36.304 303 151 10 1 280 1 284 9.25e-39 139
MsG0880043313.01.T01 MTR_2g036240 36.304 303 151 10 1 280 1 284 9.25e-39 139
MsG0880043313.01.T01 MTR_2g036260 36.304 303 151 10 1 280 1 284 9.25e-39 139
MsG0880043313.01.T01 MTR_2g036290 36.304 303 151 10 1 280 1 284 9.25e-39 139
MsG0880043313.01.T01 MTR_8g046730 36.304 303 151 10 1 280 1 284 9.25e-39 139
MsG0880043313.01.T01 MTR_6g079630 34.307 274 155 8 13 280 33 287 4.56e-37 134
MsG0880043313.01.T01 MTR_2g096030 32.990 291 163 10 14 291 31 302 2.39e-33 124
MsG0880043313.01.T01 MTR_2g096040 32.806 253 146 6 39 283 26 262 7.62e-28 108
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 71 sgRNAs with CRISPR-Local

Find 80 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAGGGAATATAAAAGATTT+TGG 0.113401 8:+22420942 None:intergenic
AATCGATTTCCATGTCATTT+TGG 0.208877 8:-22420904 MsG0880043313.01.T01:CDS
ACCACAGTGACGATATTAAT+TGG 0.223661 8:-22420431 MsG0880043313.01.T01:CDS
ATTTCGTTGTAGTCAATATC+AGG 0.268052 8:+22420924 None:intergenic
GGAGAGAAAGGATATTTAGT+AGG 0.309124 8:+22420687 None:intergenic
ATCCAAGACTACACGTTTGA+TGG 0.357506 8:-22420612 MsG0880043313.01.T01:CDS
TGTAGTGTTCATTAGTTTCA+TGG 0.361129 8:+22420469 None:intergenic
GAGAGAAAGGATATTTAGTA+GGG 0.368934 8:+22420688 None:intergenic
GTTGGAGAAATCTTCTTCAT+TGG 0.372094 8:+22420560 None:intergenic
GAATCGATCAACAGTGTTAC+CGG 0.376698 8:+22420998 None:intergenic
AAGATTTCTCCAACTACGTT+GGG 0.393833 8:-22420551 MsG0880043313.01.T01:CDS
CATCTTCGTTGTAAGTCTCA+TGG 0.421031 8:+22420874 None:intergenic
TACAAAGTCAGTCTCGTTAG+TGG 0.444755 8:+22420377 None:intergenic
GTAGTGTTCATTAGTTTCAT+GGG 0.454037 8:+22420470 None:intergenic
GGTTAGGCTGCATGTAGAAT+TGG 0.458886 8:+22420400 None:intergenic
AACACTACAAGCTACTCTAC+AGG 0.460172 8:-22420455 MsG0880043313.01.T01:CDS
CGGAAAATTCTATCCCGATT+GGG 0.468618 8:-22420841 MsG0880043313.01.T01:CDS
CCACAGTGACGATATTAATT+GGG 0.471394 8:-22420430 MsG0880043313.01.T01:CDS
TGCCATCAAACGTGTAGTCT+TGG 0.476476 8:+22420610 None:intergenic
CTAAGTACCACCTTGACCTT+GGG 0.479326 8:+22420768 None:intergenic
GATCGGTGCTTGCTCCTACT+AGG 0.491839 8:+22420304 None:intergenic
GCAGATATCAAGATCCCAAT+CGG 0.494886 8:+22420827 None:intergenic
TGTTCATTAGTTTCATGGGA+TGG 0.496934 8:+22420474 None:intergenic
TATAACGTCCCCAACGTAGT+TGG 0.497459 8:+22420542 None:intergenic
TTGCGAGTCTAACATAGATC+AGG 0.504007 8:+22420352 None:intergenic
CTGCGGTATACCACGTGTGC+AGG 0.504725 8:-22420808 MsG0880043313.01.T01:CDS
CCGGAAAATTCTATCCCGAT+TGG 0.509755 8:-22420842 MsG0880043313.01.T01:CDS
GCTAAGTACCACCTTGACCT+TGG 0.518197 8:+22420767 None:intergenic
ACAAAGTCAGTCTCGTTAGT+GGG 0.526403 8:+22420378 None:intergenic
ACGAAGACATCTAGTGGAAG+TGG 0.532647 8:+22420261 None:intergenic
CATGAGACTTACAACGAAGA+TGG 0.533162 8:-22420873 MsG0880043313.01.T01:CDS
ATCTTCGTTGTAAGTCTCAT+GGG 0.537135 8:+22420875 None:intergenic
GAGAAAGGATATTTAGTAGG+GGG 0.545897 8:+22420690 None:intergenic
ACTTACAACGAAGATGGAAA+AGG 0.547716 8:-22420867 MsG0880043313.01.T01:CDS
GTCAAGGTGGTACTTAGCAT+TGG 0.558729 8:-22420762 MsG0880043313.01.T01:CDS
TGATCTATGTTAGACTCGCA+AGG 0.562801 8:-22420350 MsG0880043313.01.T01:CDS
GAAGATTTCTCCAACTACGT+TGG 0.566238 8:-22420552 MsG0880043313.01.T01:CDS
ATTACGTCCGTCACGTGGAT+CGG 0.567052 8:+22420287 None:intergenic
TCGTGATATACAAAGTGTGC+AGG 0.567577 8:-22420736 MsG0880043313.01.T01:CDS
AAGGTGGTACTTAGCATTGG+TGG 0.572579 8:-22420759 MsG0880043313.01.T01:CDS
AGTTTCATGGGATGGAGCAA+TGG 0.579271 8:+22420482 None:intergenic
TTTCGTTGTAGTCAATATCA+GGG 0.579571 8:+22420925 None:intergenic
AACGAAGATGGAAAAGGCAC+CGG 0.582283 8:-22420861 MsG0880043313.01.T01:CDS
CAGATATCAAGATCCCAATC+GGG 0.583723 8:+22420828 None:intergenic
CTCGCAACGAAGACATCTAG+TGG 0.584791 8:+22420255 None:intergenic
TCAGTCTCGTTAGTGGGGTT+AGG 0.588840 8:+22420384 None:intergenic
AGAGAAAGGATATTTAGTAG+GGG 0.590103 8:+22420689 None:intergenic
GGGCAAAGGCCAAAATGACA+TGG 0.593006 8:+22420895 None:intergenic
GCGTTGAACCTTCATGAGAT+CGG 0.594521 8:+22420227 None:intergenic
AAAAGACTCAAGGAAGAGGT+AGG 0.596213 8:-22420519 MsG0880043313.01.T01:CDS
GGAACATTACGTCCGTCACG+TGG 0.606987 8:+22420282 None:intergenic
ACAATGTCTCATCTCATCAC+CGG 0.607947 8:-22421017 MsG0880043313.01.T01:CDS
CTGGTAATTAATTATTACGA+GGG 0.608914 8:+22420139 None:intergenic
GAGTTGCTCCGATCTCATGA+AGG 0.616774 8:-22420235 MsG0880043313.01.T01:CDS
CTCATGAAGGTTCAACGCAG+TGG 0.621602 8:-22420222 MsG0880043313.01.T01:CDS
GTTGTAAGTCTCATGGGCAA+AGG 0.623453 8:+22420881 None:intergenic
GATCTATGTTAGACTCGCAA+GGG 0.624736 8:-22420349 MsG0880043313.01.T01:CDS
GCTCAAGTATCCCAAGGTCA+AGG 0.636705 8:-22420778 MsG0880043313.01.T01:CDS
CAAAGTCAGTCTCGTTAGTG+GGG 0.637774 8:+22420379 None:intergenic
ACACTACAAGCTACTCTACA+GGG 0.639330 8:-22420454 MsG0880043313.01.T01:CDS
AGCTTGATATCCTGCACACG+TGG 0.641314 8:+22420798 None:intergenic
ATCGACGGCATTATCACACC+AGG 0.643275 8:+22420650 None:intergenic
GCAAGCACCGATCCACGTGA+CGG 0.658997 8:-22420294 MsG0880043313.01.T01:CDS
AGATTTCTCCAACTACGTTG+GGG 0.668094 8:-22420550 MsG0880043313.01.T01:CDS
TATCAAGCTCAAGTATCCCA+AGG 0.668476 8:-22420784 MsG0880043313.01.T01:CDS
CCCAATTAATATCGTCACTG+TGG 0.680315 8:+22420430 None:intergenic
GCAGGATATCATCAAGCACA+AGG 0.687092 8:-22420718 MsG0880043313.01.T01:CDS
CAAGTATCCCAAGGTCAAGG+TGG 0.692556 8:-22420775 MsG0880043313.01.T01:CDS
GCTGGTAATTAATTATTACG+AGG 0.699438 8:+22420138 None:intergenic
GATTGGGATCTTGATATCTG+CGG 0.706223 8:-22420825 MsG0880043313.01.T01:CDS
GGAAGAGGTAGGTATCAATG+TGG 0.752690 8:-22420508 MsG0880043313.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATATAAAAGATTTTGGGTA+TGG + Chr8:22420215-22420234 None:intergenic 20.0%
!! CAGGGAATATAAAAGATTTT+GGG + Chr8:22420220-22420239 None:intergenic 25.0%
!! TCAGGGAATATAAAAGATTT+TGG + Chr8:22420221-22420240 None:intergenic 25.0%
!! TTCATTTTACGAGAATACAT+TGG - Chr8:22420182-22420201 MsG0880043313.01.T01:CDS 25.0%
AGAGAAAGGATATTTAGTAG+GGG + Chr8:22420474-22420493 None:intergenic 30.0%
ATTTCGTTGTAGTCAATATC+AGG + Chr8:22420239-22420258 None:intergenic 30.0%
GAGAGAAAGGATATTTAGTA+GGG + Chr8:22420475-22420494 None:intergenic 30.0%
TATAAAAAGACTCAAGGAAG+AGG - Chr8:22420637-22420656 MsG0880043313.01.T01:CDS 30.0%
TTTCGTTGTAGTCAATATCA+GGG + Chr8:22420238-22420257 None:intergenic 30.0%
! GTAGTGTTCATTAGTTTCAT+GGG + Chr8:22420693-22420712 None:intergenic 30.0%
! TGTAGTGTTCATTAGTTTCA+TGG + Chr8:22420694-22420713 None:intergenic 30.0%
!! AATCGATTTCCATGTCATTT+TGG - Chr8:22420256-22420275 MsG0880043313.01.T01:CDS 30.0%
!! ACTTTTGAAAAACTCCTAGT+AGG - Chr8:22420842-22420861 MsG0880043313.01.T01:CDS 30.0%
!!! TCTTTTTCAATAGGAGAGAA+AGG + Chr8:22420488-22420507 None:intergenic 30.0%
AAGATTTCTCCAACTACGTT+GGG - Chr8:22420609-22420628 MsG0880043313.01.T01:CDS 35.0%
ACCACAGTGACGATATTAAT+TGG - Chr8:22420729-22420748 MsG0880043313.01.T01:CDS 35.0%
ACTTACAACGAAGATGGAAA+AGG - Chr8:22420293-22420312 MsG0880043313.01.T01:CDS 35.0%
ATCTTCGTTGTAAGTCTCAT+GGG + Chr8:22420288-22420307 None:intergenic 35.0%
CCACAGTGACGATATTAATT+GGG - Chr8:22420730-22420749 MsG0880043313.01.T01:CDS 35.0%
CTCCTATTGAAAAAGAATCC+TGG - Chr8:22420492-22420511 MsG0880043313.01.T01:CDS 35.0%
GAGAAAGGATATTTAGTAGG+GGG + Chr8:22420473-22420492 None:intergenic 35.0%
GGACGTTATAAAAAGACTCA+AGG - Chr8:22420631-22420650 MsG0880043313.01.T01:CDS 35.0%
GGAGAGAAAGGATATTTAGT+AGG + Chr8:22420476-22420495 None:intergenic 35.0%
GTTGGAGAAATCTTCTTCAT+TGG + Chr8:22420603-22420622 None:intergenic 35.0%
! TGTTCATTAGTTTCATGGGA+TGG + Chr8:22420689-22420708 None:intergenic 35.0%
!!! CACCAGGATTCTTTTTCAAT+AGG + Chr8:22420497-22420516 None:intergenic 35.0%
AAAAGACTCAAGGAAGAGGT+AGG - Chr8:22420641-22420660 MsG0880043313.01.T01:CDS 40.0%
AACACTACAAGCTACTCTAC+AGG - Chr8:22420705-22420724 MsG0880043313.01.T01:CDS 40.0%
ACAAAGTCAGTCTCGTTAGT+GGG + Chr8:22420785-22420804 None:intergenic 40.0%
ACACTACAAGCTACTCTACA+GGG - Chr8:22420706-22420725 MsG0880043313.01.T01:CDS 40.0%
AGATTTCTCCAACTACGTTG+GGG - Chr8:22420610-22420629 MsG0880043313.01.T01:CDS 40.0%
ATCCAAGACTACACGTTTGA+TGG - Chr8:22420548-22420567 MsG0880043313.01.T01:CDS 40.0%
CAGATATCAAGATCCCAATC+GGG + Chr8:22420335-22420354 None:intergenic 40.0%
CATCTTCGTTGTAAGTCTCA+TGG + Chr8:22420289-22420308 None:intergenic 40.0%
CATGAGACTTACAACGAAGA+TGG - Chr8:22420287-22420306 MsG0880043313.01.T01:CDS 40.0%
CCCAATTAATATCGTCACTG+TGG + Chr8:22420733-22420752 None:intergenic 40.0%
CGGAAAATTCTATCCCGATT+GGG - Chr8:22420319-22420338 MsG0880043313.01.T01:CDS 40.0%
GAAGATTTCTCCAACTACGT+TGG - Chr8:22420608-22420627 MsG0880043313.01.T01:CDS 40.0%
GAATCGATCAACAGTGTTAC+CGG + Chr8:22420165-22420184 None:intergenic 40.0%
GATCTATGTTAGACTCGCAA+GGG - Chr8:22420811-22420830 MsG0880043313.01.T01:CDS 40.0%
GATTGGGATCTTGATATCTG+CGG - Chr8:22420335-22420354 MsG0880043313.01.T01:CDS 40.0%
GCAGATATCAAGATCCCAAT+CGG + Chr8:22420336-22420355 None:intergenic 40.0%
TACAAAGTCAGTCTCGTTAG+TGG + Chr8:22420786-22420805 None:intergenic 40.0%
TATCAAGCTCAAGTATCCCA+AGG - Chr8:22420376-22420395 MsG0880043313.01.T01:CDS 40.0%
TCGTGATATACAAAGTGTGC+AGG - Chr8:22420424-22420443 MsG0880043313.01.T01:CDS 40.0%
TGATCTATGTTAGACTCGCA+AGG - Chr8:22420810-22420829 MsG0880043313.01.T01:CDS 40.0%
TTGCGAGTCTAACATAGATC+AGG + Chr8:22420811-22420830 None:intergenic 40.0%
! AACGCAGTGGCATTTTCATT+TGG - Chr8:22420951-22420970 MsG0880043313.01.T01:CDS 40.0%
! CCAATCGGGATAGAATTTTC+CGG + Chr8:22420321-22420340 None:intergenic 40.0%
!! GATCTTTTTGAGCGAATCGA+CGG + Chr8:22420528-22420547 None:intergenic 40.0%
ACGAAGACATCTAGTGGAAG+TGG + Chr8:22420902-22420921 None:intergenic 45.0%
AGTTTCATGGGATGGAGCAA+TGG + Chr8:22420681-22420700 None:intergenic 45.0%
CAAAGTCAGTCTCGTTAGTG+GGG + Chr8:22420784-22420803 None:intergenic 45.0%
CCGGAAAATTCTATCCCGAT+TGG - Chr8:22420318-22420337 MsG0880043313.01.T01:CDS 45.0%
CTAAGTACCACCTTGACCTT+GGG + Chr8:22420395-22420414 None:intergenic 45.0%
GCAGGATATCATCAAGCACA+AGG - Chr8:22420442-22420461 MsG0880043313.01.T01:CDS 45.0%
GCGTTGAACCTTCATGAGAT+CGG + Chr8:22420936-22420955 None:intergenic 45.0%
GGAAGAGGTAGGTATCAATG+TGG - Chr8:22420652-22420671 MsG0880043313.01.T01:CDS 45.0%
GGTTAGGCTGCATGTAGAAT+TGG + Chr8:22420763-22420782 None:intergenic 45.0%
GTCAAGGTGGTACTTAGCAT+TGG - Chr8:22420398-22420417 MsG0880043313.01.T01:CDS 45.0%
GTTGTAAGTCTCATGGGCAA+AGG + Chr8:22420282-22420301 None:intergenic 45.0%
TATAACGTCCCCAACGTAGT+TGG + Chr8:22420621-22420640 None:intergenic 45.0%
TGCCATCAAACGTGTAGTCT+TGG + Chr8:22420553-22420572 None:intergenic 45.0%
! AACGAAGATGGAAAAGGCAC+CGG - Chr8:22420299-22420318 MsG0880043313.01.T01:CDS 45.0%
! AAGGTGGTACTTAGCATTGG+TGG - Chr8:22420401-22420420 MsG0880043313.01.T01:CDS 45.0%
AGCTTGATATCCTGCACACG+TGG + Chr8:22420365-22420384 None:intergenic 50.0%
ATCGACGGCATTATCACACC+AGG + Chr8:22420513-22420532 None:intergenic 50.0%
ATTACGTCCGTCACGTGGAT+CGG + Chr8:22420876-22420895 None:intergenic 50.0%
CAAGTATCCCAAGGTCAAGG+TGG - Chr8:22420385-22420404 MsG0880043313.01.T01:CDS 50.0%
CTCATGAAGGTTCAACGCAG+TGG - Chr8:22420938-22420957 MsG0880043313.01.T01:CDS 50.0%
CTCGCAACGAAGACATCTAG+TGG + Chr8:22420908-22420927 None:intergenic 50.0%
GAGTTGCTCCGATCTCATGA+AGG - Chr8:22420925-22420944 MsG0880043313.01.T01:CDS 50.0%
GCTAAGTACCACCTTGACCT+TGG + Chr8:22420396-22420415 None:intergenic 50.0%
GCTCAAGTATCCCAAGGTCA+AGG - Chr8:22420382-22420401 MsG0880043313.01.T01:CDS 50.0%
GGGCAAAGGCCAAAATGACA+TGG + Chr8:22420268-22420287 None:intergenic 50.0%
TCAGTCTCGTTAGTGGGGTT+AGG + Chr8:22420779-22420798 None:intergenic 50.0%
GGAACATTACGTCCGTCACG+TGG + Chr8:22420881-22420900 None:intergenic 55.0%
! GATCGGTGCTTGCTCCTACT+AGG + Chr8:22420859-22420878 None:intergenic 55.0%
CTGCGGTATACCACGTGTGC+AGG - Chr8:22420352-22420371 MsG0880043313.01.T01:CDS 60.0%
!! GCAAGCACCGATCCACGTGA+CGG - Chr8:22420866-22420885 MsG0880043313.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr8 gene 22420146 22421036 22420146 ID=MsG0880043313.01;Name=MsG0880043313.01
Chr8 mRNA 22420146 22421036 22420146 ID=MsG0880043313.01.T01;Parent=MsG0880043313.01;Name=MsG0880043313.01.T01;_AED=0.44;_eAED=0.44;_QI=0|-1|0|1|-1|1|1|0|296
Chr8 exon 22420146 22421036 22420146 ID=MsG0880043313.01.T01:exon:64262;Parent=MsG0880043313.01.T01
Chr8 CDS 22420146 22421036 22420146 ID=MsG0880043313.01.T01:cds;Parent=MsG0880043313.01.T01
Gene Sequence

>MsG0880043313.01.T01

ATGTCTCATCTCATCACCGGTAACACTGTTGATCGATTCATTTTACGAGAATACATTGGCGTGAAACCATACCCAAAATCTTTTATATTCCCTGATATTGACTACAACGAAATCGATTTCCATGTCATTTTGGCCTTTGCCCATGAGACTTACAACGAAGATGGAAAAGGCACCGGAAAATTCTATCCCGATTGGGATCTTGATATCTGCGGTATACCACGTGTGCAGGATATCAAGCTCAAGTATCCCAAGGTCAAGGTGGTACTTAGCATTGGTGGTCGTGATATACAAAGTGTGCAGGATATCATCAAGCACAAGGATATTCCCCCTACTAAATATCCTTTCTCTCCTATTGAAAAAGAATCCTGGTGTGATAATGCCGTCGATTCGCTCAAAAAGATCATCCAAGACTACACGTTTGATGGCATTGATATTTTCTATGACAACATCAATACCAATGAAGAAGATTTCTCCAACTACGTTGGGGACGTTATAAAAAGACTCAAGGAAGAGGTAGGTATCAATGTGGTGTCCATTGCTCCATCCCATGAAACTAATGAACACTACAAGCTACTCTACAGGGACCACAGTGACGATATTAATTGGGTTAACTACCAATTCTACATGCAGCCTAACCCCACTAACGAGACTGACTTTGTAGACCTGATCTATGTTAGACTCGCAAGGGAATGTTACACTTTTGAAAAACTCCTAGTAGGAGCAAGCACCGATCCACGTGACGGACGTAATGTTCCACTTCCACTAGATGTCTTCGTTGCGAGTTGCTCCGATCTCATGAAGGTTCAACGCAGTGGCATTTTCATTTGGAATGCTAATGACTATGAGAAAATTGCACGAGATATCATAAAAACTATCCCTCGTAATAATTAA

Protein sequence

>MsG0880043313.01.T01

MSHLITGNTVDRFILREYIGVKPYPKSFIFPDIDYNEIDFHVILAFAHETYNEDGKGTGKFYPDWDLDICGIPRVQDIKLKYPKVKVVLSIGGRDIQSVQDIIKHKDIPPTKYPFSPIEKESWCDNAVDSLKKIIQDYTFDGIDIFYDNINTNEEDFSNYVGDVIKRLKEEVGINVVSIAPSHETNEHYKLLYRDHSDDINWVNYQFYMQPNPTNETDFVDLIYVRLARECYTFEKLLVGASTDPRDGRNVPLPLDVFVASCSDLMKVQRSGIFIWNANDYEKIARDIIKTIPRNN*