AlfalfaGEDB | MsG0880044224.01

M. sativa cultivar ZhongmuNo.1 / MsG0880044224.01

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880044224.01.T01	RHN40749.1	100	88	0	0	1	88	1	88	1.20E-54	174

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880044224.01.T01	A0A396GHU3	100.000	88	0	0	1	88	1	88	5.71e-55	174

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048016.01	MsG0880044224.01	-0.850350	1.797338e-60	3.510392e-57
MsG0180000482.01	MsG0880044224.01	-0.812728	3.663491e-51	2.347201e-48
MsG0180001100.01	MsG0880044224.01	-0.808735	2.676432e-50	1.542127e-47
MsG0180001105.01	MsG0880044224.01	-0.826617	2.459060e-54	2.317896e-51
MsG0880044224.01	MsG0880047152.01	-0.800269	1.560030e-48	7.232855e-46
MsG0880044224.01	MsG0880047519.01	-0.816287	5.977987e-52	4.218704e-49
MsG0180003357.01	MsG0880044224.01	-0.807269	5.489072e-50	3.043990e-47
MsG0180004448.01	MsG0880044224.01	-0.821616	3.673607e-53	3.003456e-50
MsG0180005408.01	MsG0880044224.01	-0.810544	1.093658e-50	6.610723e-48
MsG0180005418.01	MsG0880044224.01	-0.837452	5.155196e-57	6.699592e-54
MsG0880042857.01	MsG0880044224.01	-0.811380	7.207103e-51	4.454328e-48
MsG0880042987.01	MsG0880044224.01	0.830491	2.850440e-55	3.007622e-52
MsG0880043337.01	MsG0880044224.01	0.806018	1.008199e-49	5.414115e-47
MsG0280011073.01	MsG0880044224.01	-0.806552	7.781026e-50	4.235776e-47
MsG0380013050.01	MsG0880044224.01	0.841620	4.256227e-58	6.289685e-55
MsG0380013342.01	MsG0880044224.01	-0.801754	7.757604e-49	3.734279e-46
MsG0380014538.01	MsG0880044224.01	-0.824095	9.715503e-54	8.521715e-51
MsG0380014866.01	MsG0880044224.01	-0.817629	2.985864e-52	2.185841e-49
MsG0680034235.01	MsG0880044224.01	-0.809280	2.045318e-50	1.195849e-47
MsG0480022624.01	MsG0880044224.01	-0.813651	2.298122e-51	1.509477e-48
MsG0480023384.01	MsG0880044224.01	-0.802812	4.697338e-49	2.323140e-46
MsG0480023974.01	MsG0880044224.01	0.820599	6.298920e-53	5.005885e-50
MsG0580024717.01	MsG0880044224.01	-0.805519	1.283981e-49	6.806789e-47
MsG0580024775.01	MsG0880044224.01	-0.826867	2.142546e-54	2.034125e-51
MsG0580028024.01	MsG0880044224.01	-0.824685	7.059205e-54	6.298261e-51
MsG0580028033.01	MsG0880044224.01	-0.817909	2.582091e-52	1.904801e-49
MsG0680030464.01	MsG0880044224.01	-0.812160	4.876446e-51	3.077384e-48
MsG0280008738.01	MsG0880044224.01	-0.806191	9.273236e-50	5.001794e-47
MsG0280008929.01	MsG0880044224.01	-0.807680	4.491492e-50	2.517404e-47
MsG0280008932.01	MsG0880044224.01	-0.804484	2.112559e-49	1.090511e-46
MsG0280009676.01	MsG0880044224.01	0.827725	1.334824e-54	1.299451e-51
MsG0780037330.01	MsG0880044224.01	-0.829851	4.083977e-55	4.231311e-52
MsG0780037701.01	MsG0880044224.01	-0.849573	2.965141e-60	5.645395e-57
MsG0780037946.01	MsG0880044224.01	-0.807036	6.149480e-50	3.389558e-47
MsG0780039964.01	MsG0880044224.01	-0.809005	2.343055e-50	1.359802e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 29 sgRNAs with CRISPR-Local

Find 23 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGATGAGCTATTCCGCATTA+TGG	0.264063	8:-37908873	MsG0880044224.01.T01:CDS
TGCACCTCCTCCTCCTGATT+TGG	0.313592	8:-37909011	MsG0880044224.01.T01:CDS
ATTAGTTGGACGCGCTCTTT+TGG	0.328578	8:+37909078	None:intergenic
GGCTTGCTTCGATACGCTGC+TGG	0.331694	8:+37908951	None:intergenic
GGCTCGCTTCAATGCGTTGT+TGG	0.344390	8:+37908930	None:intergenic
AAAGTACTGACCTTATTAGT+TGG	0.391151	8:+37909064	None:intergenic
AAATCACTGAGTTGGAGGTT+GGG	0.404568	8:+37908838	None:intergenic
GGTGGACATAGTAGCACGTT+TGG	0.410455	8:-37908990	MsG0880044224.01.T01:CDS
GCTACTATGTCCACCAAATC+AGG	0.411036	8:+37908998	None:intergenic
AAGAGCGCGTCCAACTAATA+AGG	0.424679	8:-37909074	MsG0880044224.01.T01:CDS
GGTGCAGGCACATTAGCAGC+AGG	0.441887	8:+37909028	None:intergenic
CAAATCACTGAGTTGGAGGT+TGG	0.444461	8:+37908837	None:intergenic
ACCTCCTCCTCCTGATTTGG+TGG	0.465473	8:-37909008	MsG0880044224.01.T01:CDS
AATCACTGAGTTGGAGGTTG+GGG	0.465605	8:+37908839	None:intergenic
GCTGCTGGCGCGCCTCGATA+CGG	0.470336	8:+37908966	None:intergenic
CAGCAGTCTCAATACGCTCT+TGG	0.493032	8:+37908909	None:intergenic
TGCAGGCACATTAGCAGCAG+GGG	0.505123	8:+37909030	None:intergenic
AAATCAGGAGGAGGAGGTGC+AGG	0.505526	8:+37909013	None:intergenic
ACAAGGACAAATCACTGAGT+TGG	0.515568	8:+37908830	None:intergenic
AGGACAAATCACTGAGTTGG+AGG	0.557604	8:+37908833	None:intergenic
TCCACCAAATCAGGAGGAGG+AGG	0.563742	8:+37909007	None:intergenic
GAGCTATTCCGCATTATGGA+TGG	0.588295	8:-37908869	MsG0880044224.01.T01:CDS
GGAATAGCTCATCAAAGCGC+TGG	0.595097	8:+37908882	None:intergenic
GTGCAGGCACATTAGCAGCA+GGG	0.608776	8:+37909029	None:intergenic
ATGTCCACCAAATCAGGAGG+AGG	0.611528	8:+37909004	None:intergenic
ATCACTGAGTTGGAGGTTGG+GGG	0.637697	8:+37908840	None:intergenic
AGCACGTTTGGACCGTATCG+AGG	0.656377	8:-37908978	MsG0880044224.01.T01:CDS
GGATTTGACCATCCATAATG+CGG	0.674802	8:+37908861	None:intergenic
ACTATGTCCACCAAATCAGG+AGG	0.698541	8:+37909001	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAGTACTGACCTTATTAGT+TGG	+	Chr8:37908865-37908884	None:intergenic	30.0%
	GGATTTGACCATCCATAATG+CGG	+	Chr8:37909068-37909087	None:intergenic	40.0%
	TGATGAGCTATTCCGCATTA+TGG	-	Chr8:37909053-37909072	MsG0880044224.01.T01:CDS	40.0%
	ACTATGTCCACCAAATCAGG+AGG	+	Chr8:37908928-37908947	None:intergenic	45.0%
	GAGCTATTCCGCATTATGGA+TGG	-	Chr8:37909057-37909076	MsG0880044224.01.T01:CDS	45.0%
	GCTACTATGTCCACCAAATC+AGG	+	Chr8:37908931-37908950	None:intergenic	45.0%
!	AAGAGCGCGTCCAACTAATA+AGG	-	Chr8:37908852-37908871	MsG0880044224.01.T01:CDS	45.0%
!	ATTAGTTGGACGCGCTCTTT+TGG	+	Chr8:37908851-37908870	None:intergenic	45.0%
	ATGTCCACCAAATCAGGAGG+AGG	+	Chr8:37908925-37908944	None:intergenic	50.0%
	CAGCAGTCTCAATACGCTCT+TGG	+	Chr8:37909020-37909039	None:intergenic	50.0%
	GGAATAGCTCATCAAAGCGC+TGG	+	Chr8:37909047-37909066	None:intergenic	50.0%
	GGTGGACATAGTAGCACGTT+TGG	-	Chr8:37908936-37908955	MsG0880044224.01.T01:CDS	50.0%
	AGCACGTTTGGACCGTATCG+AGG	-	Chr8:37908948-37908967	MsG0880044224.01.T01:CDS	55.0%
	GGCTCGCTTCAATGCGTTGT+TGG	+	Chr8:37908999-37909018	None:intergenic	55.0%
	GTGCAGGCACATTAGCAGCA+GGG	+	Chr8:37908900-37908919	None:intergenic	55.0%
	TCCACCAAATCAGGAGGAGG+AGG	+	Chr8:37908922-37908941	None:intergenic	55.0%
	TGCACCTCCTCCTCCTGATT+TGG	-	Chr8:37908915-37908934	MsG0880044224.01.T01:CDS	55.0%
	TGCAGGCACATTAGCAGCAG+GGG	+	Chr8:37908899-37908918	None:intergenic	55.0%
!	AAATCAGGAGGAGGAGGTGC+AGG	+	Chr8:37908916-37908935	None:intergenic	55.0%
!!	ACCTCCTCCTCCTGATTTGG+TGG	-	Chr8:37908918-37908937	MsG0880044224.01.T01:CDS	55.0%
	GGTGCAGGCACATTAGCAGC+AGG	+	Chr8:37908901-37908920	None:intergenic	60.0%
!	GGCTTGCTTCGATACGCTGC+TGG	+	Chr8:37908978-37908997	None:intergenic	60.0%
	GCTGCTGGCGCGCCTCGATA+CGG	+	Chr8:37908963-37908982	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
Chr8	gene	37908841	37909107	37908841	ID=MsG0880044224.01;Name=MsG0880044224.01
Chr8	mRNA	37908841	37909107	37908841	ID=MsG0880044224.01.T01;Parent=MsG0880044224.01;Name=MsG0880044224.01.T01;_AED=0.44;_eAED=0.44;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|88
Chr8	exon	37908841	37909107	37908841	ID=MsG0880044224.01.T01:exon:2732;Parent=MsG0880044224.01.T01
Chr8	CDS	37908841	37909107	37908841	ID=MsG0880044224.01.T01:cds;Parent=MsG0880044224.01.T01

Gene Sequence	>MsG0880044224.01.T01 ATGGGCACCAAAAGAGCGCGTCCAACTAATAAGGTCAGTACTTTTCGATCTGCTGCCCCTGCTGCTAATGTGCCTGCACCTCCTCCTCCTGATTTGGTGGACATAGTAGCACGTTTGGACCGTATCGAGGCGCGCCAGCAGCGTATCGAAGCAAGCCAACAACGCATTGAAGCGAGCCAAGAGCGTATTGAGACTGCTGTACACCAGCGCTTTGATGAGCTATTCCGCATTATGGATGGTCAAATCCCCCAACCTCCAACTCAGTGA
Protein sequence	>MsG0880044224.01.T01 MGTKRARPTNKVSTFRSAAPAANVPAPPPPDLVDIVARLDRIEARQQRIEASQQRIEASQERIETAVHQRFDELFRIMDGQIPQPPTQ*