Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047152.01.T01 | XP_003609761.1 | 95.671 | 231 | 10 | 0 | 1 | 231 | 1 | 231 | 3.72E-164 | 462 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048016.01 | MsG0880047152.01 | 0.876202 | 1.684575e-68 | 8.095435e-65 |
| MsG0080048464.01 | MsG0880047152.01 | 0.812171 | 4.848762e-51 | 3.060808e-48 |
| MsG0080048614.01 | MsG0880047152.01 | 0.826206 | 3.080272e-54 | 2.869770e-51 |
| MsG0080048748.01 | MsG0880047152.01 | 0.831794 | 1.363553e-55 | 1.495615e-52 |
| MsG0080048773.01 | MsG0880047152.01 | 0.817766 | 2.780797e-52 | 2.043487e-49 |
| MsG0080048802.01 | MsG0880047152.01 | 0.821579 | 3.745202e-53 | 3.058923e-50 |
| MsG0080048845.01 | MsG0880047152.01 | 0.808134 | 3.594886e-50 | 2.039114e-47 |
| MsG0080048849.01 | MsG0880047152.01 | 0.836935 | 6.991076e-57 | 8.943109e-54 |
| MsG0080048912.01 | MsG0880047152.01 | 0.812952 | 3.272880e-51 | 2.109205e-48 |
| MsG0080048938.01 | MsG0880047152.01 | 0.880197 | 6.693060e-70 | 3.736472e-66 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047152.01.T01 | MTR_4g121940 | 95.671 | 231 | 10 | 0 | 1 | 231 | 1 | 231 | 4.51e-168 | 462 |
Find 68 sgRNAs with CRISPR-Local
Find 94 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGCTGAGGAGAAGAAAAT+CGG | 0.488939 | 8:-82709263 | MsG0880047152.01.T01:CDS |
| AAAGGTCTACTAATGAAATC+AGG | 0.461485 | 8:+82707881 | None:intergenic |
| AAATCGGAATGACAGAAACA+TGG | 0.662897 | 8:-82709247 | MsG0880047152.01.T01:CDS |
| AACAAGATCAAACATTTCCA+CGG | 0.540534 | 8:+82708160 | None:intergenic |
| AAGAGAGAAGCCAACAAATG+GGG | 0.651734 | 8:-82708904 | MsG0880047152.01.T01:CDS |
| AAGGAGACTTGTGAAAGATG+GGG | 0.673941 | 8:-82708327 | MsG0880047152.01.T01:CDS |
| AAGTCTCCTTCAATTCTGGT+GGG | 0.496148 | 8:+82708340 | None:intergenic |
| AAGTTTGATGAGTCAAGATG+TGG | 0.608940 | 8:-82708451 | MsG0880047152.01.T01:CDS |
| AATAGCAAAAGCAAAAGCTG+AGG | 0.623925 | 8:-82709276 | MsG0880047152.01.T01:CDS |
| AATCTCATTCCTTCAAAACA+TGG | 0.569007 | 8:-82709148 | MsG0880047152.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AACAAGATCAAACATTTCCA+CGG | + | Chr8:82709190-82709209 | None:intergenic | 30.0% | |
| AACCTAACAAAAACTATTGC+AGG | - | Chr8:82708495-82708514 | MsG0880047152.01.T01:intron | 30.0% | |
| AATCTCATTCCTTCAAAACA+TGG | - | Chr8:82708199-82708218 | MsG0880047152.01.T01:CDS | 30.0% | |
| GAAAAGAAAAGAAAAGATGC+AGG | - | Chr8:82708047-82708066 | MsG0880047152.01.T01:three_prime_UTR | 30.0% | |
| GATGATGTGGAAGTTATATT+GGG | - | Chr8:82708383-82708402 | MsG0880047152.01.T01:CDS | 30.0% | |
| TCTAGATTAATTGAGTAAGC+TGG | - | Chr8:82709309-82709328 | MsG0880047152.01.T01:five_prime_UTR | 30.0% | |
| TTGAACATGAAGTTCATTTC+TGG | - | Chr8:82709156-82709175 | MsG0880047152.01.T01:CDS | 30.0% | |
| TTTGAAGGAATGAGATTGAA+CGG | + | Chr8:82708196-82708215 | None:intergenic | 30.0% | |
| TTTGACCTTATCTACTGTAT+AGG | - | Chr8:82708658-82708677 | MsG0880047152.01.T01:intron | 30.0% | |
| AAAGCTGAGGAGAAGAAAAT+CGG | - | Chr8:82708084-82708103 | MsG0880047152.01.T01:three_prime_UTR | 35.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 82707896 | 82709473 | 82707896 | ID=MsG0880047152.01;Name=MsG0880047152.01 |
| Chr8 | mRNA | 82707896 | 82709473 | 82707896 | ID=MsG0880047152.01.T01;Parent=MsG0880047152.01;Name=MsG0880047152.01.T01;_AED=0.49;_eAED=0.49;_QI=167|1|1|1|1|1|3|271|231 |
| Chr8 | exon | 82707896 | 82708482 | 82707896 | ID=MsG0880047152.01.T01:exon:20439;Parent=MsG0880047152.01.T01 |
| Chr8 | exon | 82708853 | 82709070 | 82708853 | ID=MsG0880047152.01.T01:exon:20438;Parent=MsG0880047152.01.T01 |
| Chr8 | exon | 82709145 | 82709473 | 82709145 | ID=MsG0880047152.01.T01:exon:20437;Parent=MsG0880047152.01.T01 |
| Chr8 | five_prime_UTR | 82709307 | 82709473 | 82709307 | ID=MsG0880047152.01.T01:five_prime_utr;Parent=MsG0880047152.01.T01 |
| Chr8 | CDS | 82709145 | 82709306 | 82709145 | ID=MsG0880047152.01.T01:cds;Parent=MsG0880047152.01.T01 |
| Chr8 | CDS | 82708853 | 82709070 | 82708853 | ID=MsG0880047152.01.T01:cds;Parent=MsG0880047152.01.T01 |
| Chr8 | CDS | 82708167 | 82708482 | 82708167 | ID=MsG0880047152.01.T01:cds;Parent=MsG0880047152.01.T01 |
| Chr8 | three_prime_UTR | 82707896 | 82708166 | 82707896 | ID=MsG0880047152.01.T01:three_prime_utr;Parent=MsG0880047152.01.T01 |
| Gene Sequence |
>MsG0880047152.01.T01 ATGCAGGTAATAGCAAAAGCAAAAGCTGAGGAGAAGAAAATCGGAATGACAGAAACATGGTTGAGGAAACACCGTCCTATCTACGACGCAGCAACCAGACACCCTTTTATTCTCTCCATTCGTGATGGCACCGTTCAATCTCATTCCTTCAAAACATGGCTCGCTCAGGATTACTTGTTTGTTAGAGAGTTTGTTCCATTTGTTGCGAGTGTGCTGATTAAAGCTTGCAAGGAATCAGATGACAGTGATGATGTGGAAGTTATATTGGGAGGTATGGCTTCTCTTAAGGATGAAATATTATGGTTTAAGAGAGAAGCCAACAAATGGGGTATTTCACTCTCAGACGTTGTTCCTCAAAAACCTAACAAAAACTATTGCAGGTTGCTGGAAAGTTTGATGAGTCAAGATGTGGATTATACCGTGGCTCTTACTGCATTTTGGGCAATTGAAGTGGTGTATCAAGAGAGTTTTGCACACTGTATTGAAGAAGGCTCAAAAACCCCACCAGAATTGAAGGAGACTTGTGAAAGATGGGGCAATGAAGGGTTTGGTCAGTACTGTCAATCTCTGCAAAACATAGTCAATCAGCGCTTGCAAAAGGCTTCTAAGGATGAGCTAAAGAAGGCTGAAGTCATGTTGCTGAGTATTATTGAACATGAAGTTCATTTCTGGAATATGAGCCGTGGAAATGTTTGA |
| Protein sequence |
>MsG0880047152.01.T01 MQVIAKAKAEEKKIGMTETWLRKHRPIYDAATRHPFILSIRDGTVQSHSFKTWLAQDYLFVREFVPFVASVLIKACKESDDSDDVEVILGGMASLKDEILWFKREANKWGISLSDVVPQKPNKNYCRLLESLMSQDVDYTVALTAFWAIEVVYQESFAHCIEEGSKTPPELKETCERWGNEGFGQYCQSLQNIVNQRLQKASKDELKKAEVMLLSIIEHEVHFWNMSRGNV* |
