Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047506.01.T01 | XP_003610171.1 | 99.296 | 142 | 1 | 0 | 1 | 142 | 65 | 206 | 2.66E-103 | 303 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047506.01.T01 | Q40374 | 57.246 | 138 | 54 | 2 | 6 | 142 | 40 | 173 | 7.54E-53 | 167 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047506.01.T01 | G7JD30 | 99.296 | 142 | 1 | 0 | 1 | 142 | 65 | 206 | 1.27e-103 | 303 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000557.01 | MsG0880047506.01 | 0.826095 | 3.273058e-54 | 3.039625e-51 |
| MsG0180001828.01 | MsG0880047506.01 | 0.803842 | 2.874031e-49 | 1.459397e-46 |
| MsG0880045294.01 | MsG0880047506.01 | 0.813731 | 2.206602e-51 | 1.452508e-48 |
| MsG0180004754.01 | MsG0880047506.01 | 0.808826 | 2.559341e-50 | 1.478240e-47 |
| MsG0880042544.01 | MsG0880047506.01 | 0.829817 | 4.163311e-55 | 4.309192e-52 |
| MsG0880042760.01 | MsG0880047506.01 | 0.800587 | 1.343666e-48 | 6.280240e-46 |
| MsG0280010907.01 | MsG0880047506.01 | 0.815121 | 1.087149e-51 | 7.430250e-49 |
| MsG0380014778.01 | MsG0880047506.01 | 0.807042 | 6.131655e-50 | 3.380231e-47 |
| MsG0480020597.01 | MsG0880047506.01 | 0.802723 | 4.899595e-49 | 2.417672e-46 |
| MsG0480022855.01 | MsG0880047506.01 | 0.802206 | 6.263291e-49 | 3.049840e-46 |
| MsG0580026059.01 | MsG0880047506.01 | 0.814389 | 1.579435e-51 | 1.058459e-48 |
| MsG0380015944.01 | MsG0880047506.01 | 0.837553 | 4.857799e-57 | 6.332397e-54 |
| MsG0580028897.01 | MsG0880047506.01 | 0.802373 | 5.785761e-49 | 2.829608e-46 |
| MsG0580029127.01 | MsG0880047506.01 | 0.803452 | 3.462243e-49 | 1.740500e-46 |
| MsG0280008764.01 | MsG0880047506.01 | 0.825597 | 4.297583e-54 | 3.935063e-51 |
| MsG0280008909.01 | MsG0880047506.01 | 0.819292 | 1.253597e-52 | 9.607192e-50 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047506.01.T01 | MTR_4g128770 | 99.296 | 142 | 1 | 0 | 1 | 142 | 65 | 206 | 3.22e-107 | 303 |
| MsG0880047506.01.T01 | MTR_4g128750 | 57.246 | 138 | 54 | 2 | 6 | 142 | 40 | 173 | 7.34e-54 | 167 |
| MsG0880047506.01.T01 | MTR_2g010630 | 47.518 | 141 | 66 | 5 | 6 | 142 | 29 | 165 | 1.00e-43 | 141 |
| MsG0880047506.01.T01 | MTR_2g010590 | 45.255 | 137 | 70 | 2 | 6 | 142 | 28 | 159 | 8.66e-43 | 139 |
| MsG0880047506.01.T01 | MTR_2g010600 | 46.377 | 138 | 70 | 3 | 6 | 142 | 28 | 162 | 3.74e-42 | 137 |
| MsG0880047506.01.T01 | MTR_4g050762 | 46.377 | 138 | 70 | 3 | 6 | 142 | 28 | 162 | 6.19e-42 | 137 |
| MsG0880047506.01.T01 | MTR_2g012370 | 46.377 | 138 | 70 | 3 | 6 | 142 | 28 | 162 | 6.19e-42 | 137 |
| MsG0880047506.01.T01 | MTR_2g010650 | 48.227 | 141 | 63 | 6 | 6 | 142 | 31 | 165 | 2.93e-40 | 132 |
| MsG0880047506.01.T01 | MTR_2g435490 | 44.928 | 138 | 70 | 3 | 6 | 142 | 32 | 164 | 1.12e-38 | 129 |
| MsG0880047506.01.T01 | MTR_2g010700 | 42.254 | 142 | 75 | 5 | 6 | 142 | 29 | 168 | 3.89e-33 | 114 |
| MsG0880047506.01.T01 | MTR_4g092540 | 42.336 | 137 | 74 | 2 | 6 | 142 | 28 | 159 | 4.20e-32 | 112 |
| MsG0880047506.01.T01 | MTR_2g010580 | 43.796 | 137 | 71 | 3 | 6 | 142 | 34 | 164 | 5.04e-32 | 112 |
| MsG0880047506.01.T01 | MTR_2g010640 | 41.135 | 141 | 73 | 7 | 6 | 142 | 29 | 163 | 1.15e-31 | 110 |
| MsG0880047506.01.T01 | MTR_1g067290 | 42.143 | 140 | 72 | 5 | 6 | 141 | 73 | 207 | 2.29e-30 | 108 |
| MsG0880047506.01.T01 | MTR_2g010610 | 50.495 | 101 | 43 | 4 | 43 | 142 | 44 | 138 | 5.41e-30 | 105 |
| MsG0880047506.01.T01 | MTR_2g010690 | 40.141 | 142 | 74 | 7 | 6 | 142 | 29 | 164 | 3.71e-27 | 99.8 |
| MsG0880047506.01.T01 | MTR_2g010670 | 39.716 | 141 | 76 | 6 | 6 | 142 | 29 | 164 | 4.46e-27 | 99.4 |
| MsG0880047506.01.T01 | MTR_8g078770 | 35.915 | 142 | 89 | 2 | 2 | 142 | 32 | 172 | 1.89e-24 | 92.8 |
| MsG0880047506.01.T01 | MTR_5g018770 | 32.374 | 139 | 92 | 1 | 6 | 142 | 47 | 185 | 3.48e-23 | 89.7 |
| MsG0880047506.01.T01 | MTR_5g018755 | 33.696 | 92 | 56 | 3 | 56 | 142 | 12 | 103 | 1.54e-12 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047506.01.T01 | AT4G25780 | 65.000 | 140 | 49 | 0 | 3 | 142 | 51 | 190 | 1.69e-72 | 215 |
| MsG0880047506.01.T01 | AT4G25790 | 56.522 | 138 | 55 | 2 | 6 | 142 | 77 | 210 | 2.18e-52 | 165 |
| MsG0880047506.01.T01 | AT5G57625 | 54.745 | 137 | 57 | 2 | 7 | 142 | 75 | 207 | 4.03e-50 | 159 |
| MsG0880047506.01.T01 | AT4G30320 | 50.725 | 138 | 62 | 3 | 6 | 142 | 29 | 161 | 7.01e-47 | 149 |
| MsG0880047506.01.T01 | AT1G01310 | 49.635 | 137 | 65 | 1 | 6 | 142 | 87 | 219 | 2.53e-45 | 148 |
| MsG0880047506.01.T01 | AT4G31470 | 51.095 | 137 | 63 | 1 | 6 | 142 | 53 | 185 | 1.29e-44 | 144 |
| MsG0880047506.01.T01 | AT4G33720 | 49.635 | 137 | 64 | 2 | 6 | 142 | 32 | 163 | 1.25e-40 | 134 |
| MsG0880047506.01.T01 | AT5G02730 | 46.479 | 142 | 70 | 3 | 3 | 142 | 56 | 193 | 1.34e-39 | 132 |
| MsG0880047506.01.T01 | AT3G19690 | 45.890 | 146 | 70 | 4 | 1 | 142 | 21 | 161 | 1.48e-39 | 130 |
| MsG0880047506.01.T01 | AT2G14610 | 43.066 | 137 | 71 | 3 | 6 | 142 | 32 | 161 | 1.60e-37 | 125 |
| MsG0880047506.01.T01 | AT3G09590 | 46.809 | 141 | 69 | 3 | 4 | 142 | 50 | 186 | 3.99e-37 | 125 |
| MsG0880047506.01.T01 | AT5G26130 | 44.526 | 137 | 73 | 2 | 6 | 142 | 33 | 166 | 4.10e-36 | 122 |
| MsG0880047506.01.T01 | AT2G14580 | 40.876 | 137 | 74 | 2 | 6 | 142 | 32 | 161 | 4.34e-35 | 119 |
| MsG0880047506.01.T01 | AT1G50050 | 40.876 | 137 | 78 | 1 | 6 | 142 | 29 | 162 | 8.05e-34 | 116 |
| MsG0880047506.01.T01 | AT4G33730 | 41.304 | 138 | 74 | 4 | 6 | 142 | 41 | 172 | 1.44e-33 | 116 |
| MsG0880047506.01.T01 | AT4G33710 | 40.876 | 137 | 78 | 2 | 6 | 142 | 33 | 166 | 8.30e-31 | 108 |
| MsG0880047506.01.T01 | AT1G50060 | 43.066 | 137 | 74 | 1 | 6 | 142 | 29 | 161 | 9.92e-31 | 108 |
| MsG0880047506.01.T01 | AT2G19990 | 41.304 | 138 | 75 | 3 | 6 | 142 | 44 | 176 | 3.35e-30 | 107 |
| MsG0880047506.01.T01 | AT1G50050 | 40.157 | 127 | 73 | 1 | 6 | 132 | 29 | 152 | 3.95e-29 | 106 |
| MsG0880047506.01.T01 | AT5G66590 | 34.266 | 143 | 91 | 3 | 2 | 142 | 44 | 185 | 1.38e-24 | 93.6 |
| MsG0880047506.01.T01 | AT4G07820 | 33.333 | 132 | 81 | 4 | 11 | 142 | 36 | 160 | 2.51e-19 | 79.3 |
| MsG0880047506.01.T01 | AT2G19980 | 36.667 | 150 | 67 | 10 | 3 | 142 | 34 | 165 | 2.20e-15 | 69.3 |
Find 33 sgRNAs with CRISPR-Local
Find 34 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTGAAGATGGTTTCAAATT+AGG | 0.121429 | 8:-87259030 | MsG0880047506.01.T01:CDS |
| TATGATTAATTAAATTAATA+TGG | 0.291226 | 8:+87258760 | None:intergenic |
| GAATGTTCCACTTTGCAATC+TGG | 0.345438 | 8:+87259057 | None:intergenic |
| ATTAGGAGAGAATATATATT+GGG | 0.348184 | 8:-87259013 | MsG0880047506.01.T01:CDS |
| CCTAATTTGAAACCATCTTC+AGG | 0.349768 | 8:+87259030 | None:intergenic |
| AATTAGGAGAGAATATATAT+TGG | 0.371913 | 8:-87259014 | MsG0880047506.01.T01:CDS |
| GTGACTAATTCATGTGTATC+TGG | 0.422128 | 8:-87258919 | MsG0880047506.01.T01:CDS |
| CATCAATGGAAGTTCCCATT+TGG | 0.423106 | 8:+87259131 | None:intergenic |
| TGGAAAAGTACTAGAAGAAT+TGG | 0.425934 | 8:-87258865 | MsG0880047506.01.T01:CDS |
| CAACGGATGCTGTAAAAGCT+TGG | 0.458076 | 8:-87258969 | MsG0880047506.01.T01:CDS |
| ATTTAGTAAGAGCATCCAAA+TGG | 0.467344 | 8:-87259146 | MsG0880047506.01.T01:CDS |
| AGCTGGTAATCCCACATCAA+TGG | 0.480192 | 8:+87259117 | None:intergenic |
| AACGGATGCTGTAAAAGCTT+GGG | 0.485815 | 8:-87258968 | MsG0880047506.01.T01:CDS |
| GAGAATATATATTGGGGTAG+TGG | 0.490490 | 8:-87259006 | MsG0880047506.01.T01:CDS |
| TTGAACAATACGCAAGGTGG+TGG | 0.491083 | 8:-87259095 | MsG0880047506.01.T01:CDS |
| ATTGGGGTAGTGGCTCTGAT+TGG | 0.492371 | 8:-87258996 | MsG0880047506.01.T01:CDS |
| GCAAGAGTTGTGTGTGATGA+TGG | 0.498907 | 8:-87258838 | MsG0880047506.01.T01:CDS |
| TCTCCAACGTAATTACCAAC+AGG | 0.517002 | 8:+87258787 | None:intergenic |
| ACCTTGCGTATTGTTCAAGC+TGG | 0.537913 | 8:+87259100 | None:intergenic |
| TTTAGTAAGAGCATCCAAAT+GGG | 0.554322 | 8:-87259145 | MsG0880047506.01.T01:CDS |
| AATGGGAACTTCCATTGATG+TGG | 0.559795 | 8:-87259128 | MsG0880047506.01.T01:CDS |
| CAAGGTGGTGGGCTAGTCAA+AGG | 0.567063 | 8:-87259083 | MsG0880047506.01.T01:CDS |
| GATCCTGTTGGTAATTACGT+TGG | 0.567456 | 8:-87258790 | MsG0880047506.01.T01:CDS |
| ATGGGAACTTCCATTGATGT+GGG | 0.586774 | 8:-87259127 | MsG0880047506.01.T01:CDS |
| TGAACAATACGCAAGGTGGT+GGG | 0.616960 | 8:-87259094 | MsG0880047506.01.T01:CDS |
| GACATTATACTCAGATTGTG+TGG | 0.618377 | 8:-87258885 | MsG0880047506.01.T01:CDS |
| TTAGGAGAGAATATATATTG+GGG | 0.623586 | 8:-87259012 | MsG0880047506.01.T01:CDS |
| TGGCTCTGATTGGACACCAA+CGG | 0.632245 | 8:-87258986 | MsG0880047506.01.T01:CDS |
| TGTGTATCTGGTCAAATGTG+TGG | 0.656492 | 8:-87258907 | MsG0880047506.01.T01:CDS |
| ACCAGCTTGAACAATACGCA+AGG | 0.661122 | 8:-87259101 | MsG0880047506.01.T01:CDS |
| ACTTGTAACTATGATCCTGT+TGG | 0.661520 | 8:-87258802 | MsG0880047506.01.T01:CDS |
| AGCTTGAACAATACGCAAGG+TGG | 0.696278 | 8:-87259098 | MsG0880047506.01.T01:CDS |
| AAGGAAACCAGATTGCAAAG+TGG | 0.700454 | 8:-87259064 | MsG0880047506.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTAGGAGAGAATATATAT+TGG | - | Chr8:87258940-87258959 | MsG0880047506.01.T01:CDS | 20.0% |
| !! | ATTAGGAGAGAATATATATT+GGG | - | Chr8:87258941-87258960 | MsG0880047506.01.T01:CDS | 20.0% |
| ! | TTAGGAGAGAATATATATTG+GGG | - | Chr8:87258942-87258961 | MsG0880047506.01.T01:CDS | 25.0% |
| !! | ATTTAGTAAGAGCATCCAAA+TGG | - | Chr8:87258808-87258827 | MsG0880047506.01.T01:CDS | 30.0% |
| !! | TGGAAAAGTACTAGAAGAAT+TGG | - | Chr8:87259089-87259108 | MsG0880047506.01.T01:CDS | 30.0% |
| !! | TTTAGTAAGAGCATCCAAAT+GGG | - | Chr8:87258809-87258828 | MsG0880047506.01.T01:CDS | 30.0% |
| ACTTGTAACTATGATCCTGT+TGG | - | Chr8:87259152-87259171 | MsG0880047506.01.T01:CDS | 35.0% | |
| CCTGAAGATGGTTTCAAATT+AGG | - | Chr8:87258924-87258943 | MsG0880047506.01.T01:CDS | 35.0% | |
| GACATTATACTCAGATTGTG+TGG | - | Chr8:87259069-87259088 | MsG0880047506.01.T01:CDS | 35.0% | |
| GAGAATATATATTGGGGTAG+TGG | - | Chr8:87258948-87258967 | MsG0880047506.01.T01:CDS | 35.0% | |
| GTGACTAATTCATGTGTATC+TGG | - | Chr8:87259035-87259054 | MsG0880047506.01.T01:CDS | 35.0% | |
| ! | CCTAATTTGAAACCATCTTC+AGG | + | Chr8:87258927-87258946 | None:intergenic | 35.0% |
| ! | GAACATTCTTTTCCTGAAGA+TGG | - | Chr8:87258912-87258931 | MsG0880047506.01.T01:CDS | 35.0% |
| AACGGATGCTGTAAAAGCTT+GGG | - | Chr8:87258986-87259005 | MsG0880047506.01.T01:CDS | 40.0% | |
| AAGGAAACCAGATTGCAAAG+TGG | - | Chr8:87258890-87258909 | MsG0880047506.01.T01:CDS | 40.0% | |
| AATGGGAACTTCCATTGATG+TGG | - | Chr8:87258826-87258845 | MsG0880047506.01.T01:CDS | 40.0% | |
| ATGGGAACTTCCATTGATGT+GGG | - | Chr8:87258827-87258846 | MsG0880047506.01.T01:CDS | 40.0% | |
| CATCAATGGAAGTTCCCATT+TGG | + | Chr8:87258826-87258845 | None:intergenic | 40.0% | |
| GAATGTTCCACTTTGCAATC+TGG | + | Chr8:87258900-87258919 | None:intergenic | 40.0% | |
| TCTCCAACGTAATTACCAAC+AGG | + | Chr8:87259170-87259189 | None:intergenic | 40.0% | |
| TGTGTATCTGGTCAAATGTG+TGG | - | Chr8:87259047-87259066 | MsG0880047506.01.T01:CDS | 40.0% | |
| !! | GATCCTGTTGGTAATTACGT+TGG | - | Chr8:87259164-87259183 | MsG0880047506.01.T01:CDS | 40.0% |
| ACCAGCTTGAACAATACGCA+AGG | - | Chr8:87258853-87258872 | MsG0880047506.01.T01:CDS | 45.0% | |
| ACCTTGCGTATTGTTCAAGC+TGG | + | Chr8:87258857-87258876 | None:intergenic | 45.0% | |
| AGCTGGTAATCCCACATCAA+TGG | + | Chr8:87258840-87258859 | None:intergenic | 45.0% | |
| AGCTTGAACAATACGCAAGG+TGG | - | Chr8:87258856-87258875 | MsG0880047506.01.T01:CDS | 45.0% | |
| CAACGGATGCTGTAAAAGCT+TGG | - | Chr8:87258985-87259004 | MsG0880047506.01.T01:CDS | 45.0% | |
| GCAAGAGTTGTGTGTGATGA+TGG | - | Chr8:87259116-87259135 | MsG0880047506.01.T01:CDS | 45.0% | |
| TGAACAATACGCAAGGTGGT+GGG | - | Chr8:87258860-87258879 | MsG0880047506.01.T01:CDS | 45.0% | |
| TTGAACAATACGCAAGGTGG+TGG | - | Chr8:87258859-87258878 | MsG0880047506.01.T01:CDS | 45.0% | |
| ! | CAAGCTTTTACAGCATCCGT+TGG | + | Chr8:87258987-87259006 | None:intergenic | 45.0% |
| !! | ATTGGGGTAGTGGCTCTGAT+TGG | - | Chr8:87258958-87258977 | MsG0880047506.01.T01:CDS | 50.0% |
| !! | TGGCTCTGATTGGACACCAA+CGG | - | Chr8:87258968-87258987 | MsG0880047506.01.T01:CDS | 50.0% |
| !! | CAAGGTGGTGGGCTAGTCAA+AGG | - | Chr8:87258871-87258890 | MsG0880047506.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 87258774 | 87259202 | 87258774 | ID=MsG0880047506.01;Name=MsG0880047506.01 |
| Chr8 | mRNA | 87258774 | 87259202 | 87258774 | ID=MsG0880047506.01.T01;Parent=MsG0880047506.01;Name=MsG0880047506.01.T01;_AED=0.24;_eAED=0.24;_QI=0|-1|0|1|-1|1|1|0|142 |
| Chr8 | exon | 87258774 | 87259202 | 87258774 | ID=MsG0880047506.01.T01:exon:15761;Parent=MsG0880047506.01.T01 |
| Chr8 | CDS | 87258774 | 87259202 | 87258774 | ID=MsG0880047506.01.T01:cds;Parent=MsG0880047506.01.T01 |
| Gene Sequence |
| Protein sequence |
