AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180001828.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180001828.01.T01 MTR_1g044580 96.207 290 11 0 11 300 1 290 0.0 574
MsG0180001828.01.T01 MTR_1g073320 73.929 280 61 2 34 301 20 299 4.91e-137 392
MsG0180001828.01.T01 MTR_7g098410 46.809 282 140 5 20 300 10 282 8.83e-81 248
MsG0180001828.01.T01 MTR_1g080350 38.433 268 153 6 36 293 24 289 9.07e-57 186
MsG0180001828.01.T01 MTR_8g104990 33.582 268 171 6 38 301 18 282 1.16e-35 131
MsG0180001828.01.T01 MTR_5g078030 31.599 269 165 8 38 299 20 276 4.07e-28 110
MsG0180001828.01.T01 MTR_3g065370 30.375 293 178 9 8 297 3 272 3.05e-26 105
MsG0180001828.01.T01 MTR_1g044560 43.689 103 58 0 152 254 24 126 4.37e-20 85.1
MsG0180001828.01.T01 MTR_7g052020 26.291 213 147 3 50 253 28 239 8.61e-17 79.3
MsG0180001828.01.T01 MTR_1g104890 25.191 262 171 8 50 291 45 301 6.47e-15 74.7
MsG0180001828.01.T01 MTR_6g055960 25.424 236 150 4 59 268 36 271 1.28e-14 75.5
MsG0180001828.01.T01 MTR_1g105655 23.860 285 184 7 49 311 40 313 1.43e-14 75.1
MsG0180001828.01.T01 MTR_1g104890 25.191 262 171 8 50 291 45 301 2.28e-14 73.9
MsG0180001828.01.T01 MTR_1g104890 25.191 262 171 8 50 291 45 301 3.53e-14 73.9
MsG0180001828.01.T01 MTR_1g104890 25.191 262 171 8 50 291 45 301 4.07e-14 73.6
MsG0180001828.01.T01 MTR_5g005480 27.103 214 141 4 50 249 35 247 4.88e-14 73.6
MsG0180001828.01.T01 MTR_6g463400 25.121 207 139 5 58 249 42 247 2.70e-13 70.9
MsG0180001828.01.T01 MTR_5g068260 26.238 202 135 5 59 248 42 241 2.03e-12 68.6
MsG0180001828.01.T01 MTR_1g105755 24.481 241 128 6 59 272 46 259 1.03e-11 66.2
MsG0180001828.01.T01 MTR_1g105820 23.849 239 135 5 59 272 46 262 1.06e-11 66.2
MsG0180001828.01.T01 MTR_6g463830 24.528 265 165 9 35 268 18 278 1.19e-11 66.2
MsG0180001828.01.T01 MTR_2g010710 24.747 198 139 4 59 248 43 238 2.60e-11 63.5
MsG0180001828.01.T01 MTR_1g105650 26.407 231 133 8 44 249 33 251 2.73e-11 65.1
MsG0180001828.01.T01 MTR_6g463660 23.913 184 126 3 80 249 65 248 3.39e-11 64.7
MsG0180001828.01.T01 MTR_6g057680 23.913 184 126 3 80 249 65 248 3.39e-11 64.7
MsG0180001828.01.T01 MTR_2g010730 26.923 234 147 8 62 276 51 279 4.25e-11 63.2
MsG0180001828.01.T01 MTR_6g053000 27.477 222 137 8 16 224 5 215 6.28e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180001828.01.T01 AT1G04520 62.914 302 94 4 19 303 7 307 2.41e-124 359
MsG0180001828.01.T01 AT2G33330 60.535 299 104 4 13 299 4 300 6.79e-120 348
MsG0180001828.01.T01 AT5G43980 41.343 283 150 4 39 306 22 303 4.96e-68 216
MsG0180001828.01.T01 AT3G04370 35.599 309 168 5 19 298 8 314 2.43e-59 194
MsG0180001828.01.T01 AT3G04370 36.013 311 165 6 19 298 8 315 4.13e-59 193
MsG0180001828.01.T01 AT3G60720 31.439 264 159 7 45 298 29 280 3.74e-32 122
MsG0180001828.01.T01 AT3G60720 31.538 260 156 7 45 294 29 276 1.58e-31 120
MsG0180001828.01.T01 AT5G37660 29.927 274 180 5 9 280 4 267 7.42e-29 113
MsG0180001828.01.T01 AT5G37660 29.927 274 180 5 9 280 4 267 1.16e-28 112
MsG0180001828.01.T01 AT2G01660 28.283 297 193 7 13 301 2 286 1.36e-28 112
MsG0180001828.01.T01 AT2G01660 28.283 297 193 7 13 301 2 286 1.60e-28 112
MsG0180001828.01.T01 AT3G60720 29.694 229 141 5 45 266 29 244 1.07e-24 100
MsG0180001828.01.T01 AT2G01660 26.587 252 168 6 13 259 2 241 8.84e-23 95.9
MsG0180001828.01.T01 AT1G70690 26.962 293 191 10 19 298 9 291 8.32e-21 91.3
MsG0180001828.01.T01 AT5G48540 25.969 258 148 7 46 282 24 259 5.87e-17 80.1
MsG0180001828.01.T01 AT5G41290 24.303 251 165 5 14 248 3 244 1.51e-15 76.3
MsG0180001828.01.T01 AT4G05200 26.482 253 161 6 10 249 6 246 4.47e-13 70.5
MsG0180001828.01.T01 AT4G05200 25.692 253 163 6 10 249 24 264 4.48e-13 70.5

Find 53 sgRNAs with CRISPR-Local

Find 139 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATCTGCATACCTGATATT+TGG 0.131490 1:+27816515 None:intergenic
GCTTTATCAGCGATGGAAAA+TGG 0.195405 1:-27815645 MsG0180001828.01.T01:CDS
CAAAATTTGTTGGACAAAAT+TGG 0.199965 1:+27816607 None:intergenic
TAGAATAAATGAAAGAAATA+TGG 0.209647 1:+27816906 None:intergenic
GCTGCAAGAGTTCAACTTCT+TGG 0.248312 1:-27816571 MsG0180001828.01.T01:CDS
GAATGTGGCAACTCTATTTC+TGG 0.253750 1:-27815492 MsG0180001828.01.T01:CDS
ACAAACCAGTTATACTGTTT+TGG 0.290553 1:+27816686 None:intergenic
CATAAGTGTTTCATTAGTTA+TGG 0.313486 1:-27815456 MsG0180001828.01.T01:CDS
TATCTTCCACAGATTATTGA+TGG 0.332558 1:-27814650 None:intergenic
AATGTTGCTGGAAGTGGTTT+TGG 0.333457 1:-27815684 MsG0180001828.01.T01:CDS
GCACAATGTGAAGGTGATAT+TGG 0.371110 1:-27815567 MsG0180001828.01.T01:CDS
CTTTATCAGCGATGGAAAAT+GGG 0.376842 1:-27815644 MsG0180001828.01.T01:CDS
AATCTGCATACCTGATATTT+GGG 0.386006 1:+27816516 None:intergenic
GCTGGTTATACCCAAATATC+AGG 0.389407 1:-27816526 MsG0180001828.01.T01:intron
AGTGGCCAAAACAGTATAAC+TGG 0.391004 1:-27816691 MsG0180001828.01.T01:CDS
GTTTGTGAGTATATTCCATT+TGG 0.392368 1:+27816784 None:intergenic
TATCTACTATATGAAGTTGC+TGG 0.393316 1:-27816544 MsG0180001828.01.T01:CDS
TGTCTAGATGGAACTCCATT+AGG 0.401134 1:+27815426 None:intergenic
AATGGGGTTGTTAGTGGTCA+TGG 0.418001 1:-27815627 MsG0180001828.01.T01:CDS
AGTTATGGCTATTATCCTAA+TGG 0.427351 1:-27815441 MsG0180001828.01.T01:CDS
CAAACACTTTCTACACTCCT+TGG 0.431163 1:-27816766 MsG0180001828.01.T01:CDS
AGAATAAATGAAAGAAATAT+GGG 0.443988 1:+27816907 None:intergenic
GCAATGACAGGGCAAGATCC+AGG 0.447894 1:-27814858 MsG0180001828.01.T01:intron
ATAATACTAGGAGGAATTGC+TGG 0.462780 1:-27814822 MsG0180001828.01.T01:CDS
TGTGGGATAACAAATGTTGC+TGG 0.470043 1:-27815696 MsG0180001828.01.T01:CDS
GAATATGAAGAATGTCTAGA+TGG 0.470370 1:+27815414 None:intergenic
AAATTCTATAAAACCACTAC+TGG 0.477222 1:-27816715 MsG0180001828.01.T01:CDS
ATGGAAAATGGGGTTGTTAG+TGG 0.485449 1:-27815633 MsG0180001828.01.T01:CDS
GTTCTTACCTGAAGAAAATG+AGG 0.485674 1:+27815392 None:intergenic
TTATATTCTTGCTATTTGCT+AGG 0.498339 1:-27814785 MsG0180001828.01.T01:CDS
CTGGTTTGTTCCAATGTAGA+GGG 0.509794 1:-27816672 MsG0180001828.01.T01:CDS
ATAACAAATGTTGCTGGAAG+TGG 0.510378 1:-27815690 MsG0180001828.01.T01:CDS
GATATTGGTGATTCTGATTG+TGG 0.528519 1:-27815552 MsG0180001828.01.T01:CDS
AAAACAGCTGCTATAATACT+AGG 0.532912 1:-27814834 MsG0180001828.01.T01:CDS
CAGATGCTCTATAAGACTTG+TGG 0.533238 1:-27815714 MsG0180001828.01.T01:CDS
AGATGCTCTATAAGACTTGT+GGG 0.545982 1:-27815713 MsG0180001828.01.T01:CDS
TATAAAACCACTACTGGTAG+TGG 0.547424 1:-27816709 MsG0180001828.01.T01:CDS
ACTGGTTTGTTCCAATGTAG+AGG 0.558068 1:-27816673 MsG0180001828.01.T01:CDS
GAAGAAGATTCTGCAAAATG+AGG 0.561722 1:+27816859 None:intergenic
AGACGCTGCTTTATCAGCGA+TGG 0.565779 1:-27815652 MsG0180001828.01.T01:CDS
GAAGATTCTGCAAAATGAGG+TGG 0.568669 1:+27816862 None:intergenic
TAGTGAGATCCCCTCTACAT+TGG 0.590991 1:+27816662 None:intergenic
TACACTACATTAGTCTACAA+AGG 0.596329 1:-27816832 MsG0180001828.01.T01:CDS
ACTAGGAGGAATTGCTGGAG+TGG 0.599765 1:-27814817 MsG0180001828.01.T01:CDS
AAGATTCTGCAAAATGAGGT+GGG 0.604435 1:+27816863 None:intergenic
CAATGACAGGGCAAGATCCA+GGG 0.608246 1:-27814857 MsG0180001828.01.T01:intron
CAAAGAGCTCAAGTTGAATG+TGG 0.620507 1:-27815507 MsG0180001828.01.T01:CDS
ACAGCTGCTATAATACTAGG+AGG 0.630493 1:-27814831 MsG0180001828.01.T01:CDS
AGCATGCCATCAATAATCTG+TGG 0.638216 1:+27814644 None:intergenic
TTTATCAGCGATGGAAAATG+GGG 0.640047 1:-27815643 MsG0180001828.01.T01:CDS
TGGTTTGTTCCAATGTAGAG+GGG 0.663909 1:-27816671 MsG0180001828.01.T01:CDS
AATTTGACAAAGAAACACAA+TGG 0.727029 1:-27814762 MsG0180001828.01.T01:CDS
ATTGTGAAACTAGTGAACCA+AGG 0.728041 1:+27816749 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATATAAATATAATAAGTG+GGG - Chr1:27815306-27815325 MsG0180001828.01.T01:intron 10.0%
!! TCAATAATAATTTGAAAAAA+TGG - Chr1:27815188-27815207 MsG0180001828.01.T01:intron 10.0%
!! AGAATAAATGAAAGAAATAT+GGG + Chr1:27814704-27814723 None:intergenic 15.0%
!! ATTATAAAAAAATTCAACGA+CGG - Chr1:27815341-27815360 MsG0180001828.01.T01:intron 15.0%
!! TAGAATAAATGAAAGAAATA+TGG + Chr1:27814705-27814724 None:intergenic 15.0%
!!! TAAAATATCAATTTAGTTCA+TGG - Chr1:27816411-27816430 MsG0180001828.01.T01:intron 15.0%
!!! TTTTTATACAACAAACTATA+TGG - Chr1:27816004-27816023 MsG0180001828.01.T01:intron 15.0%
!! AAAATAAGTGTCAATTTGTA+TGG - Chr1:27816289-27816308 MsG0180001828.01.T01:intron 20.0%
!! AGAGATTAAATTAAAGTTAG+TGG + Chr1:27815283-27815302 None:intergenic 20.0%
!!! ATTTTGAAATATTGATGATG+TGG - Chr1:27816629-27816648 MsG0180001828.01.T01:CDS 20.0%
!!! GGTTTTATAGAATTTTGTTT+TGG + Chr1:27814888-27814907 None:intergenic 20.0%
!!! TTTATAGAATTTTGTTTTGG+TGG + Chr1:27814885-27814904 None:intergenic 20.0%
! AAATAAGTAAGTGGATGTAA+TGG - Chr1:27816463-27816482 MsG0180001828.01.T01:intron 25.0%
! AAATTCTATAAAACCACTAC+TGG - Chr1:27814893-27814912 MsG0180001828.01.T01:intron 25.0%
! AATTTGACAAAGAAACACAA+TGG - Chr1:27816846-27816865 MsG0180001828.01.T01:CDS 25.0%
! ATAAAATCGGTCAAAATAGT+CGG + Chr1:27815696-27815715 None:intergenic 25.0%
! CATAAGTGTTTCATTAGTTA+TGG - Chr1:27816152-27816171 MsG0180001828.01.T01:intron 25.0%
! CTTAACCATTGGAAAATTTA+GGG + Chr1:27816684-27816703 None:intergenic 25.0%
! GCGTATGAAGTATATAAAAT+CGG + Chr1:27815709-27815728 None:intergenic 25.0%
! TAACCAACATTTGTCATAAA+GGG + Chr1:27816347-27816366 None:intergenic 25.0%
! TGATAATAATTTCAAACCGA+GGG - Chr1:27816571-27816590 MsG0180001828.01.T01:CDS 25.0%
! TTAACCAACATTTGTCATAA+AGG + Chr1:27816348-27816367 None:intergenic 25.0%
! TTTAGTGTTTAAACTATCAG+TGG - Chr1:27815138-27815157 MsG0180001828.01.T01:intron 25.0%
! TTTCATAATTAGTGCTAAGA+TGG - Chr1:27815401-27815420 MsG0180001828.01.T01:CDS 25.0%
!! AAGATGAATTTGAAACTAAG+TGG - Chr1:27816529-27816548 MsG0180001828.01.T01:CDS 25.0%
!! ATGTAATGGTTGATATTTGT+AGG - Chr1:27816477-27816496 MsG0180001828.01.T01:intron 25.0%
!! ATGTCATGAGTTTTTACTTT+CGG + Chr1:27815380-27815399 None:intergenic 25.0%
!! ATTTTGTCCAACAAATTTTG+TGG - Chr1:27815004-27815023 MsG0180001828.01.T01:intron 25.0%
!! CTATATGGCATTTTATGTTT+TGG - Chr1:27816019-27816038 MsG0180001828.01.T01:intron 25.0%
!! GAAACTTTTATAGATCCAAA+TGG - Chr1:27814809-27814828 MsG0180001828.01.T01:CDS 25.0%
!! TGAAAGAAATATGGGTTTTA+AGG + Chr1:27814696-27814715 None:intergenic 25.0%
!! TTGATAATAATTTCAAACCG+AGG - Chr1:27816570-27816589 MsG0180001828.01.T01:CDS 25.0%
!!! ACAAGTTTTGTACAAATGTT+AGG - Chr1:27815867-27815886 MsG0180001828.01.T01:intron 25.0%
!!! CAAAATTTGTTGGACAAAAT+TGG + Chr1:27815004-27815023 None:intergenic 25.0%
!!! CTTATTTTTGGTGATCAATT+TGG + Chr1:27816450-27816469 None:intergenic 25.0%
!!! TACATCCACTTACTTATTTT+TGG + Chr1:27816462-27816481 None:intergenic 25.0%
!!! TTATATTCTTGCTATTTGCT+AGG - Chr1:27816823-27816842 MsG0180001828.01.T01:CDS 25.0%
AAAACAGCTGCTATAATACT+AGG - Chr1:27816774-27816793 MsG0180001828.01.T01:CDS 30.0%
AAATCTGCATACCTGATATT+TGG + Chr1:27815096-27815115 None:intergenic 30.0%
AATCTGCATACCTGATATTT+GGG + Chr1:27815095-27815114 None:intergenic 30.0%
ACATAGTTCAAATTCTTACG+AGG + Chr1:27815807-27815826 None:intergenic 30.0%
AGTTATGGCTATTATCCTAA+TGG - Chr1:27816167-27816186 MsG0180001828.01.T01:intron 30.0%
CCTTAACCATTGGAAAATTT+AGG + Chr1:27816685-27816704 None:intergenic 30.0%
CTAATGTTAAAACCTACAGA+TGG - Chr1:27816238-27816257 MsG0180001828.01.T01:intron 30.0%
GAATATGAAGAATGTCTAGA+TGG + Chr1:27816197-27816216 None:intergenic 30.0%
GATCACCAAAAATAAGTAAG+TGG - Chr1:27816454-27816473 MsG0180001828.01.T01:intron 30.0%
GTTTGTGAGTATATTCCATT+TGG + Chr1:27814827-27814846 None:intergenic 30.0%
TAAAATCGGTCAAAATAGTC+GGG + Chr1:27815695-27815714 None:intergenic 30.0%
TAGAACGACTCAATTGAAAA+GGG - Chr1:27815744-27815763 MsG0180001828.01.T01:intron 30.0%
TATCTACTATATGAAGTTGC+TGG - Chr1:27815064-27815083 MsG0180001828.01.T01:intron 30.0%
TATGGACAACTGCTTAAAAT+AGG + Chr1:27815591-27815610 None:intergenic 30.0%
TTCCAATTGTAAAAATGACG+AGG - Chr1:27815507-27815526 MsG0180001828.01.T01:CDS 30.0%
! ACAAACCAGTTATACTGTTT+TGG + Chr1:27814925-27814944 None:intergenic 30.0%
! AGTTTTGCCACAAAATTTGT+TGG + Chr1:27815014-27815033 None:intergenic 30.0%
! CCTAAATTTTCCAATGGTTA+AGG - Chr1:27816682-27816701 MsG0180001828.01.T01:CDS 30.0%
! CTTTTCCAAACACCAAATTT+TGG + Chr1:27816266-27816285 None:intergenic 30.0%
! TACACTACATTAGTCTACAA+AGG - Chr1:27814776-27814795 MsG0180001828.01.T01:CDS 30.0%
! TCATATTCCTCATTTTCTTC+AGG - Chr1:27816209-27816228 MsG0180001828.01.T01:intron 30.0%
! TTTTCCAATGGTTAAGGAAA+AGG - Chr1:27816688-27816707 MsG0180001828.01.T01:CDS 30.0%
!! ATTGATTTGATGCAATGACA+GGG - Chr1:27816739-27816758 MsG0180001828.01.T01:CDS 30.0%
!! CAAAATTTGGTGTTTGGAAA+AGG - Chr1:27816264-27816283 MsG0180001828.01.T01:intron 30.0%
!! TATTGATTTGATGCAATGAC+AGG - Chr1:27816738-27816757 MsG0180001828.01.T01:CDS 30.0%
!!! TTGCTTTTTGTGTAGTAGTA+AGG + Chr1:27814662-27814681 None:intergenic 30.0%
AACATTTGTCATAAAGGGCA+AGG + Chr1:27816342-27816361 None:intergenic 35.0%
AAGATTCTGCAAAATGAGGT+GGG + Chr1:27814748-27814767 None:intergenic 35.0%
AGAACGACTCAATTGAAAAG+GGG - Chr1:27815745-27815764 MsG0180001828.01.T01:intron 35.0%
AGATGCTCTATAAGACTTGT+GGG - Chr1:27815895-27815914 MsG0180001828.01.T01:intron 35.0%
AGTGTTTAAACTATCAGTGG+TGG - Chr1:27815141-27815160 MsG0180001828.01.T01:intron 35.0%
ATAACAAATGTTGCTGGAAG+TGG - Chr1:27815918-27815937 MsG0180001828.01.T01:intron 35.0%
ATAATACTAGGAGGAATTGC+TGG - Chr1:27816786-27816805 MsG0180001828.01.T01:CDS 35.0%
ATATAGACCTCCTTCATAAG+TGG + Chr1:27815619-27815638 None:intergenic 35.0%
ATTGTGAAACTAGTGAACCA+AGG + Chr1:27814862-27814881 None:intergenic 35.0%
CTACAGATGGAACCAAAATT+TGG - Chr1:27816251-27816270 MsG0180001828.01.T01:intron 35.0%
CTTACAAGATCGAACTCTTT+CGG - Chr1:27815836-27815855 MsG0180001828.01.T01:intron 35.0%
CTTTATCAGCGATGGAAAAT+GGG - Chr1:27815964-27815983 MsG0180001828.01.T01:intron 35.0%
GAAGAAGATTCTGCAAAATG+AGG + Chr1:27814752-27814771 None:intergenic 35.0%
GAATGTGTAATCGGTCAATT+TGG - Chr1:27815668-27815687 MsG0180001828.01.T01:CDS 35.0%
GTAGAACGACTCAATTGAAA+AGG - Chr1:27815743-27815762 MsG0180001828.01.T01:intron 35.0%
GTTCTTACCTGAAGAAAATG+AGG + Chr1:27816219-27816238 None:intergenic 35.0%
TATAAAACCACTACTGGTAG+TGG - Chr1:27814899-27814918 MsG0180001828.01.T01:intron 35.0%
TTGCCCTTTATGACAAATGT+TGG - Chr1:27816341-27816360 MsG0180001828.01.T01:intron 35.0%
TTTATCAGCGATGGAAAATG+GGG - Chr1:27815965-27815984 MsG0180001828.01.T01:intron 35.0%
! AAACTATCAGTGGTGGAAAA+TGG - Chr1:27815148-27815167 MsG0180001828.01.T01:intron 35.0%
! AACTATCAGTGGTGGAAAAT+GGG - Chr1:27815149-27815168 MsG0180001828.01.T01:intron 35.0%
! AGTACCTTTTCCTTAACCAT+TGG + Chr1:27816695-27816714 None:intergenic 35.0%
! CTCTTGGTAGTAGATGTTAA+TGG + Chr1:27815534-27815553 None:intergenic 35.0%
! GATATTGGTGATTCTGATTG+TGG - Chr1:27816056-27816075 MsG0180001828.01.T01:intron 35.0%
! GTCTCTGAAGAATGTGTAAT+CGG - Chr1:27815659-27815678 MsG0180001828.01.T01:CDS 35.0%
! TTTTAAGCAGTTGTCCATAG+TGG - Chr1:27815592-27815611 MsG0180001828.01.T01:CDS 35.0%
! TTTTAATCGAGTTTGTCCCT+CGG + Chr1:27816590-27816609 None:intergenic 35.0%
!! TGGAACCAAAATTTGGTGTT+TGG - Chr1:27816258-27816277 MsG0180001828.01.T01:intron 35.0%
!!! CAAATTTTGGTTCCATCTGT+AGG + Chr1:27816253-27816272 None:intergenic 35.0%
!!! GTCCTCGTCATTTTTACAAT+TGG + Chr1:27815512-27815531 None:intergenic 35.0%
!!! TATGTTTTGGCACAATGTGA+AGG - Chr1:27816032-27816051 MsG0180001828.01.T01:intron 35.0%
ACAGCTGCTATAATACTAGG+AGG - Chr1:27816777-27816796 MsG0180001828.01.T01:CDS 40.0%
AGTGGCCAAAACAGTATAAC+TGG - Chr1:27814917-27814936 MsG0180001828.01.T01:intron 40.0%
ATGGAAAATGGGGTTGTTAG+TGG - Chr1:27815975-27815994 MsG0180001828.01.T01:intron 40.0%
CAAACACTTTCTACACTCCT+TGG - Chr1:27814842-27814861 MsG0180001828.01.T01:CDS 40.0%
CAGATGCTCTATAAGACTTG+TGG - Chr1:27815894-27815913 MsG0180001828.01.T01:intron 40.0%
GAACGACTCAATTGAAAAGG+GGG - Chr1:27815746-27815765 MsG0180001828.01.T01:intron 40.0%
GAAGATTCTGCAAAATGAGG+TGG + Chr1:27814749-27814768 None:intergenic 40.0%
GAATGTGGCAACTCTATTTC+TGG - Chr1:27816116-27816135 MsG0180001828.01.T01:intron 40.0%
GATGGTGACTAGATTGTTGT+TGG - Chr1:27815419-27815438 MsG0180001828.01.T01:CDS 40.0%
GCACAATGTGAAGGTGATAT+TGG - Chr1:27816041-27816060 MsG0180001828.01.T01:intron 40.0%
GCTGGTTATACCCAAATATC+AGG - Chr1:27815082-27815101 MsG0180001828.01.T01:intron 40.0%
GCTTTATCAGCGATGGAAAA+TGG - Chr1:27815963-27815982 MsG0180001828.01.T01:intron 40.0%
TGTCTAGATGGAACTCCATT+AGG + Chr1:27816185-27816204 None:intergenic 40.0%
TGTGGGATAACAAATGTTGC+TGG - Chr1:27815912-27815931 MsG0180001828.01.T01:intron 40.0%
! AATGTTGCTGGAAGTGGTTT+TGG - Chr1:27815924-27815943 MsG0180001828.01.T01:intron 40.0%
! ACTATCAGTGGTGGAAAATG+GGG - Chr1:27815150-27815169 MsG0180001828.01.T01:intron 40.0%
! ATGTTGCTGGAAGTGGTTTT+GGG - Chr1:27815925-27815944 MsG0180001828.01.T01:intron 40.0%
! CTGCTCCCTAAATTTTCCAA+TGG - Chr1:27816676-27816695 MsG0180001828.01.T01:CDS 40.0%
!! ACTGGTTTGTTCCAATGTAG+AGG - Chr1:27814935-27814954 MsG0180001828.01.T01:intron 40.0%
!! ATTATAGCAGCTGTTTTCCC+TGG + Chr1:27816771-27816790 None:intergenic 40.0%
!! CAAAGAGCTCAAGTTGAATG+TGG - Chr1:27816101-27816120 MsG0180001828.01.T01:intron 40.0%
!! CTGGTTTGTTCCAATGTAGA+GGG - Chr1:27814936-27814955 MsG0180001828.01.T01:intron 40.0%
!! TGGTTTGTTCCAATGTAGAG+GGG - Chr1:27814937-27814956 MsG0180001828.01.T01:intron 40.0%
AATGGGGTTGTTAGTGGTCA+TGG - Chr1:27815981-27816000 MsG0180001828.01.T01:intron 45.0%
ACAGACATCACATCGTCTCT+TGG + Chr1:27815550-27815569 None:intergenic 45.0%
CCTTCATAAGTGGTCCACTA+TGG + Chr1:27815609-27815628 None:intergenic 45.0%
GCTGCAAGAGTTCAACTTCT+TGG - Chr1:27815037-27815056 MsG0180001828.01.T01:intron 45.0%
TAGTGAGATCCCCTCTACAT+TGG + Chr1:27814949-27814968 None:intergenic 45.0%
TGTGATGTCTGTCATAGACG+AGG - Chr1:27815558-27815577 MsG0180001828.01.T01:CDS 45.0%
! TAGTGGACCACTTATGAAGG+AGG - Chr1:27815609-27815628 MsG0180001828.01.T01:CDS 45.0%
!! CCATAGTGGACCACTTATGA+AGG - Chr1:27815606-27815625 MsG0180001828.01.T01:CDS 45.0%
!! TGTTGCTGGAAGTGGTTTTG+GGG - Chr1:27815926-27815945 MsG0180001828.01.T01:intron 45.0%
!! AAATATATAAATATAATAAG+TGG - Chr1:27815304-27815323 MsG0180001828.01.T01:intron 5.0%
!! AATATATAAATATAATAAGT+GGG - Chr1:27815305-27815324 MsG0180001828.01.T01:intron 5.0%
ACTAGGAGGAATTGCTGGAG+TGG - Chr1:27816791-27816810 MsG0180001828.01.T01:CDS 50.0%
CAATGACAGGGCAAGATCCA+GGG - Chr1:27816751-27816770 MsG0180001828.01.T01:CDS 50.0%
! ACGTCGTCCATCAAAAGTGC+TGG - Chr1:27815252-27815271 MsG0180001828.01.T01:intron 50.0%
! AGACGCTGCTTTATCAGCGA+TGG - Chr1:27815956-27815975 MsG0180001828.01.T01:intron 50.0%
!! CACTTTTGATGGACGACGTG+TGG + Chr1:27815251-27815270 None:intergenic 50.0%
!! CGTCGTCCATCAAAAGTGCT+GGG - Chr1:27815253-27815272 MsG0180001828.01.T01:intron 50.0%
!! GTCGTCCATCAAAAGTGCTG+GGG - Chr1:27815254-27815273 MsG0180001828.01.T01:intron 50.0%
!! GTTTTGGCCACTACCAGTAG+TGG + Chr1:27814909-27814928 None:intergenic 50.0%
GCAATGACAGGGCAAGATCC+AGG - Chr1:27816750-27816769 MsG0180001828.01.T01:CDS 55.0%
GTGATCACGCGTGTCTGTGT+CGG - Chr1:27815222-27815241 MsG0180001828.01.T01:intron 55.0%
! GGTAGCCCCAGCACTTTTGA+TGG + Chr1:27815262-27815281 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr1 gene 27814653 27816977 27814653 ID=MsG0180001828.01;Name=MsG0180001828.01
Chr1 mRNA 27814653 27816977 27814653 ID=MsG0180001828.01.T01;Parent=MsG0180001828.01;Name=MsG0180001828.01.T01;_AED=0.34;_eAED=0.42;_QI=0|1|0.33|1|1|1|3|0|336
Chr1 exon 27816527 27816977 27816527 ID=MsG0180001828.01.T01:exon:387;Parent=MsG0180001828.01.T01
Chr1 exon 27815400 27815741 27815400 ID=MsG0180001828.01.T01:exon:386;Parent=MsG0180001828.01.T01
Chr1 exon 27814653 27814870 27814653 ID=MsG0180001828.01.T01:exon:385;Parent=MsG0180001828.01.T01
Chr1 CDS 27816527 27816977 27816527 ID=MsG0180001828.01.T01:cds;Parent=MsG0180001828.01.T01
Chr1 CDS 27815400 27815741 27815400 ID=MsG0180001828.01.T01:cds;Parent=MsG0180001828.01.T01
Chr1 CDS 27814653 27814870 27814653 ID=MsG0180001828.01.T01:cds;Parent=MsG0180001828.01.T01
Gene Sequence

>MsG0180001828.01.T01

TTGATACCTTACTACTACACAAAAAGCAAAATGAGTTTCACCTTAAAACCCATATTTCTTTCATTTATTCTAGCTTTATTAACTAATCTTAACCCACCTCATTTTGCAGAATCTTCTTCAGATTACACTACATTAGTCTACAAAGGTTGTTCAAAAGAAACTTTTATAGATCCAAATGGAATATACTCACAAACACTTTCTACACTCCTTGGTTCACTAGTTTCACAATCCACCAAAACAAAATTCTATAAAACCACTACTGGTAGTGGCCAAAACAGTATAACTGGTTTGTTCCAATGTAGAGGGGATCTCACTAATTCTGTTTGCTACAATTGTGTAAAGAAACTACCAATTTTGTCCAACAAATTTTGTGGCAAAACTATTGCTGCAAGAGTTCAACTTCTTGGTTGTTATCTACTATATGAAGTTGCTGGTTATACCCAAATATCAGGTATGCAGATGCTCTATAAGACTTGTGGGATAACAAATGTTGCTGGAAGTGGTTTTGGGGAGAGAAGAGACGCTGCTTTATCAGCGATGGAAAATGGGGTTGTTAGTGGTCATGGTTTTTATACAACAAACTATATGGCATTTTATGTTTTGGCACAATGTGAAGGTGATATTGGTGATTCTGATTGTGGACAGTGTGTGAAAAATGCTGTTCAAAGAGCTCAAGTTGAATGTGGCAACTCTATTTCTGGTGAAATCTTTTTGCATAAGTGTTTCATTAGTTATGGCTATTATCCTAATGGAGTTCCATCTAGACATTCTTCATATTCCTCATTTTCTTCAGGGCAAGATCCAGGGAAAACAGCTGCTATAATACTAGGAGGAATTGCTGGAGTGGCTTTTCTAGTTATATTCTTGCTATTTGCTAGGAATTTGACAAAGAAACACAATGGTATGTATGAGTTATTTATGAACTCTTATGTTTATATTTTAGTCTCATCTCATGTTTTGATTCTTATTTACTCATTCTGCAATATCATTTTATCTTCCACAGATTATTGA
Protein sequence

>MsG0180001828.01.T01

LIPYYYTKSKMSFTLKPIFLSFILALLTNLNPPHFAESSSDYTTLVYKGCSKETFIDPNGIYSQTLSTLLGSLVSQSTKTKFYKTTTGSGQNSITGLFQCRGDLTNSVCYNCVKKLPILSNKFCGKTIAARVQLLGCYLLYEVAGYTQISGMQMLYKTCGITNVAGSGFGERRDAALSAMENGVVSGHGFYTTNYMAFYVLAQCEGDIGDSDCGQCVKNAVQRAQVECGNSISGEIFLHKCFISYGYYPNGVPSRHSSYSSFSSGQDPGKTAAIILGGIAGVAFLVIFLLFARNLTKKHNGMYELFMNSYVYILVSSHVLILIYSFCNIILSSTDY*