AlfalfaGEDB | Msa0076640

M. sativa cultivar zhongmu-4 / Msa0076640

KEGG: -
GO: -
PFAM: PF14543.9

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0076640	KAF7804763.1	55.981	209	42	4	1	159	176	384	2.16e-59	214
Msa0076640	KAF7804763.1	61.937	444	109	8	161	553	1	435	2.57e-177	517

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0076640	sp\|P0DO21\|GIP2_NICBE	55.804	448	134	9	161	553	1	439	1.77e-153	449
Msa0076640	sp\|P0DO21\|GIP2_NICBE	51.869	214	49	5	1	162	180	391	1.47e-55	196

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0076640	G7IEE3	59.722	216	31	4	1	161	188	402	2.33e-64	226
Msa0076640	G7IEE3	83.738	412	20	2	189	553	40	451	0.0	681

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000610	Msa0076640	-0.824409	8.200240e-54	-8.615850e-47
Msa0011920	Msa0076640	-0.832602	8.609202e-56	-8.615850e-47
Msa0029010	Msa0076640	0.874466	6.607935e-68	-8.615850e-47
Msa0030050	Msa0076640	0.802573	5.261856e-49	-8.615850e-47
Msa0030760	Msa0076640	-0.801836	7.461080e-49	-8.615850e-47
Msa0030800	Msa0076640	0.815388	9.482778e-52	-8.615850e-47
Msa0033140	Msa0076640	0.805423	1.344834e-49	-8.615850e-47
Msa0037180	Msa0076640	0.840299	9.456662e-58	-8.615850e-47
Msa0037280	Msa0076640	0.849743	2.657597e-60	-8.615850e-47
Msa0037720	Msa0076640	0.811447	6.967919e-51	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0076640	MtrunA17_Chr1g0185651	83.738	412	20	2	189	553	40	451	0.0	681
Msa0076640	MtrunA17_Chr1g0185651	59.722	216	31	4	1	161	188	402	4.48e-68	226
Msa0076640	MtrunA17_Chr1g0185661	72.748	444	63	7	160	553	651	1086	0.0	618
Msa0076640	MtrunA17_Chr1g0185661	69.816	434	72	7	161	544	1	425	0.0	572
Msa0076640	MtrunA17_Chr1g0185661	73.430	207	8	2	1	160	830	1036	3.23e-84	283
Msa0076640	MtrunA17_Chr1g0185661	68.599	207	17	4	1	160	179	384	1.02e-74	256
Msa0076640	MtrunA17_Chr2g0287821	45.622	434	173	9	171	552	35	457	4.96e-119	359
Msa0076640	MtrunA17_Chr2g0287821	45.116	215	65	5	1	164	200	412	3.53e-45	165
Msa0076640	MtrunA17_Chr1g0187841	45.169	445	157	10	173	552	13	435	1.84e-112	341
Msa0076640	MtrunA17_Chr1g0187841	47.343	207	59	5	1	160	169	372	1.05e-48	174

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0076640	AT1G03220.1	53.103	435	145	7	169	553	8	433	2.86e-141	416
Msa0076640	AT1G03220.1	48.325	209	60	4	1	161	176	384	9.76e-51	180
Msa0076640	AT1G03230.1	51.306	421	146	7	183	553	23	434	1.03e-130	389
Msa0076640	AT1G03230.1	45.455	209	66	4	1	161	177	385	1.90e-47	171
Msa0076640	AT5G19120.1	34.509	397	196	13	169	533	11	375	5.49e-45	163
Msa0076640	AT5G19120.1	31.383	188	94	6	3	165	173	350	1.52e-14	75.9
Msa0076640	AT5G19110.1	30.311	386	221	11	193	544	27	398	2.95e-40	151
Msa0076640	AT5G19110.1	29.630	189	85	7	14	160	175	357	2.03e-12	69.7
Msa0076640	AT5G19110.2	30.311	386	221	11	193	544	63	434	3.59e-40	151
Msa0076640	AT5G19110.2	29.630	189	85	7	14	160	211	393	2.54e-12	69.3

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Find 123 sgRNAs with CRISPR-Local

Find 720 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGGCATCTAGGCAGTGG+AGG	0.650082	1_2:+52816679	None:intergenic
AAAATGAAAATGTGGTTTGG+AGG	0.560548	1_2:-52825779	Msa0076640:CDS
AAACATAAAGAGGGTGGATT+CGG	0.425811	1_2:-52825883	Msa0076640:CDS
AAACTATAACAAAATTCAAA+AGG	0.494523	1_2:+52816989	None:intergenic
AAAGAATGGAAATGAAGTGT+TGG	0.528151	1_2:+52821914	None:intergenic
AAAGCCGAAGCTAGTTGAGA+TGG	0.563385	1_2:+52826275	None:intergenic
AAAGCTGAAGCTGTGCTAAC+AGG	0.402137	1_2:+52821193	None:intergenic
AACCTTGTCGTCGATCTTGG+CGG	0.500614	1_2:-52821754	Msa0076640:CDS
AAGGTTTAGTGGGACTAGTG+GGG	0.651701	1_2:+52821771	None:intergenic
AATGAAGAGCAATAAAAGAA+TGG	0.521779	1_2:+52821900	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TATTTGTCTTTCCTCTTCAA+AGG	-	chr1_2:52816765-52816784	Msa0076640:CDS	30.0%
ATTTGTCTTTCCTCTTCAAA+GGG	-	chr1_2:52816766-52816785	Msa0076640:CDS	30.0%
TCTGAATCAAGAACAATGTT+GGG	+	chr1_2:52816829-52816848	None:intergenic	30.0%
TTTGTTACACTAATGTGACT+GGG	-	chr1_2:52817151-52817170	Msa0076640:intron	30.0%
TGCAAAATGAAAATGTGGTT+TGG	-	chr1_2:52817214-52817233	Msa0076640:intron	30.0%
AATTCATGTATGGTTTGACA+AGG	-	chr1_2:52817759-52817778	Msa0076640:intron	30.0%
AAAGGGGAAAATACATTCTT+TGG	+	chr1_2:52817806-52817825	None:intergenic	30.0%
CCAACCATTTGTATAAAAAG+GGG	+	chr1_2:52817822-52817841	None:intergenic	30.0%
AATAGTGATCAAGCCAAAAT+TGG	-	chr1_2:52818281-52818300	Msa0076640:intron	30.0%
TTGGAGGATAATATAGTACT+AGG	+	chr1_2:52818778-52818797	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_2	gene	52816688	52826330	52816688	ID=Msa0076640;Name=Msa0076640
chr1_2	mRNA	52816688	52826330	52816688	ID=Msa0076640-mRNA-1;Parent=Msa0076640;Name=Msa0076640-mRNA-1;_AED=0.21;_eAED=0.22;_QI=0\|0\|0\|1\|0\|0\|6\|0\|553
chr1_2	exon	52826201	52826330	52826201	ID=Msa0076640-mRNA-1:exon:16347;Parent=Msa0076640-mRNA-1
chr1_2	exon	52825988	52826107	52825988	ID=Msa0076640-mRNA-1:exon:16346;Parent=Msa0076640-mRNA-1
chr1_2	exon	52825712	52825939	52825712	ID=Msa0076640-mRNA-1:exon:16345;Parent=Msa0076640-mRNA-1
chr1_2	exon	52821428	52821955	52821428	ID=Msa0076640-mRNA-1:exon:16344;Parent=Msa0076640-mRNA-1
chr1_2	exon	52821126	52821328	52821126	ID=Msa0076640-mRNA-1:exon:16343;Parent=Msa0076640-mRNA-1
chr1_2	exon	52816688	52817140	52816688	ID=Msa0076640-mRNA-1:exon:16342;Parent=Msa0076640-mRNA-1
chr1_2	CDS	52826201	52826330	52826201	ID=Msa0076640-mRNA-1:cds;Parent=Msa0076640-mRNA-1
chr1_2	CDS	52825988	52826107	52825988	ID=Msa0076640-mRNA-1:cds;Parent=Msa0076640-mRNA-1
chr1_2	CDS	52825712	52825939	52825712	ID=Msa0076640-mRNA-1:cds;Parent=Msa0076640-mRNA-1
chr1_2	CDS	52821428	52821955	52821428	ID=Msa0076640-mRNA-1:cds;Parent=Msa0076640-mRNA-1
chr1_2	CDS	52821126	52821328	52821126	ID=Msa0076640-mRNA-1:cds;Parent=Msa0076640-mRNA-1
chr1_2	CDS	52816688	52817140	52816688	ID=Msa0076640-mRNA-1:cds;Parent=Msa0076640-mRNA-1

Gene Sequence

>Msa0076640

ATGGCTGGTCTTGGAAGAACCAAAATTGCTCTTCCATCTCAACTAGCTTCGGCTTTTAGCTTCGATAGAAAATTCGCTATTTGTCTTTCCTCTTCAAAGGGAGTTGTTTTATTCGGGGATGGACCTTATGGTCAGCCTTCAGCCGAGTATTTCATCGGCGTGAAAACAATAAAAATCGATGAAAAAGTTGTTTCACTTAACTCAAGTTTGTTTTCTATAGACAACAACGGCGTTGGTGGAACAAAGATTATTACAGACGCTTTCGTGAAAGCGTCTGCTGCAAGAAACATAAAGAGGGTGGATTCGGTTGCGCCTTTTGAATTTTGTTACACTAATGTGACTGGGACACGATTGGGTGCAGCTGTGCCTACTATTGAGCTCTTTTTGCAAAATGAAAATGTGGTTTGGAGGATATTTGGTGCAAATTCAATGGTGAATATAAATGATGAGGTTTTGTGTCTTGGATTTGTGAATGGTGCCATGGCTAATACCAACTTCCAACACTTCATTTCCATTCTTTTATTGCTCTTCATTACACCAACTTTCTCTCAACAATCATTCAGACCCAAAGCACTTGTTCTCCCTGTCACAAAAGATAGCGCAACAACACTACAATACTTGGCTCAAATTAACCAAAGAACCCCACTAGTCCCACTAAACCTTGTCGTCGATCTTGGCGGCAAATTTCTCTGGGTCGACTGTGAAAACCACTACACTTCCTCTACATACCACCCTGCACGGTGCCCCTCCGCCCAATGTTCCCTCGCCAAATCTGATAGCTGCGGTGATTGTTTCTCATCACCAAAACCCGGCTGCAACAACACTTGTGGCCTCATTCCGGACAACACCATCACACACAGCGCTACTCGTGGCGAACTAGCTGAAGACGTTTTATCCATCCAATCAACTAACGGTTTCAACCCAGGACAAAATGTAGTTGTTTCACGCTTTCTATTTTCATGTGCACCGACTTCATTATTACGAGGACTTGCAGGTGGTGTTTCTGATGGTGTCATCATCTTCGGTGATGGTCCTTACAGTTTTCTTGCTGACAACGCTTCTCTCCCTAACGTTGTATTTGATTCAAAATCACTCACATACACGCCACTTTTAATCAATCCTGTTAGCACAGCTTCAGCTTTCTTACAGGGAGAGCCTTCAGTCGAGTATTTCATCGGAGTGAAAACAATAAAAATTGATGGAAAAGTGTTAGGTGGAACAAAGATTAGTACGGTTGATCCTTACACGGTTTTAGAAGCTTCTATTTATAAAGCAGTCACAGACGCTTTTGTGAAAGCGTCTGTTGCAAGAAACATAACGACGGAGGATTCATCTCCGCCTTTTGAATTTTGTTATAGTTTTGACAATTTGCCAGGGACGCCATTGGGTGCGGCTGTGCCTACAATTGAGCTTGTTTTACAGAACAATGTGATTTGGAGCATGTTTGGTGCAAATTCAATGGTGAATATAAATGATGAGGTTTTGTGCCTTGGATTTGTTAACGGTGGTGAGAATGCAAGGACTTCTATTGTGATTGGAGGGTATCAATTGGAGAATAATGTTCTGCAATTTGATTTGGCTGCGTCTAGATTGGGTTTTAGCAACACGATCTTTGCACGTCAAACTGATTGTTTTAGATTCAACTTCACCTCCACTGCCTAG

Protein sequence

>Msa0076640

MAGLGRTKIALPSQLASAFSFDRKFAICLSSSKGVVLFGDGPYGQPSAEYFIGVKTIKIDEKVVSLNSSLFSIDNNGVGGTKIITDAFVKASAARNIKRVDSVAPFEFCYTNVTGTRLGAAVPTIELFLQNENVVWRIFGANSMVNINDEVLCLGFVNGAMANTNFQHFISILLLLFITPTFSQQSFRPKALVLPVTKDSATTLQYLAQINQRTPLVPLNLVVDLGGKFLWVDCENHYTSSTYHPARCPSAQCSLAKSDSCGDCFSSPKPGCNNTCGLIPDNTITHSATRGELAEDVLSIQSTNGFNPGQNVVVSRFLFSCAPTSLLRGLAGGVSDGVIIFGDGPYSFLADNASLPNVVFDSKSLTYTPLLINPVSTASAFLQGEPSVEYFIGVKTIKIDGKVLGGTKISTVDPYTVLEASIYKAVTDAFVKASVARNITTEDSSPPFEFCYSFDNLPGTPLGAAVPTIELVLQNNVIWSMFGANSMVNINDEVLCLGFVNGGENARTSIVIGGYQLENNVLQFDLAASRLGFSNTIFARQTDCFRFNFTSTA*