AlfalfaGEDB | Msa0101750

M. sativa cultivar zhongmu-4 / Msa0101750

KEGG: -
GO: -
PFAM: PF02362.24

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0101750	XP_003589266.1	98.490	596	9	0	1	596	1	596	0.0	1228

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0101750	sp\|Q8S2E6\|Y1235_ORYSJ	26.913	379	222	11	12	350	17	380	4.96e-28	119
Msa0101750	sp\|Q8S2E6\|Y1235_ORYSJ	37.607	117	66	2	463	576	265	377	4.82e-15	80.9

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0101750	G7I4K0	98.490	596	9	0	1	596	1	596	0.0	1228

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TFs/TRs:

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Gene ID	Type	Classification
Msa0101750	TF	B3

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0101750	Msa0336290	0.810382	1.184916e-50	-8.615850e-47
Msa0101750	Msa0411390	0.803600	3.226679e-49	-8.615850e-47
Msa0101750	Msa0530560	0.832259	1.046729e-55	-8.615850e-47
Msa0101750	Msa0534230	0.813974	1.950824e-51	-8.615850e-47
Msa0101750	Msa0578920	0.807726	4.390266e-50	-8.615850e-47
Msa0101750	Msa0610880	0.808855	2.523050e-50	-8.615850e-47
Msa0101750	Msa0692040	-0.804736	1.871769e-49	-8.615850e-47
Msa0101750	Msa0765910	0.841164	5.608054e-58	-8.615850e-47
Msa0101750	Msa0832340	0.802758	4.820255e-49	-8.615850e-47
Msa0101750	Msa1061270	0.805897	1.069187e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0101750	MtrunA17_Chr1g0154041	98.490	596	9	0	1	596	1	596	0.0	1228
Msa0101750	MtrunA17_Chr1g0154101	77.720	386	80	2	209	593	460	840	0.0	602
Msa0101750	MtrunA17_Chr1g0154101	33.434	332	173	8	47	362	539	838	7.26e-41	159
Msa0101750	MtrunA17_Chr1g0154101	40.196	102	59	2	15	115	183	283	1.28e-16	84.0
Msa0101750	MtrunA17_Chr1g0154101	35.156	128	71	4	15	130	24	151	4.41e-15	79.0
Msa0101750	MtrunA17_Chr1g0154071	48.817	338	147	8	29	355	169	491	4.05e-87	279
Msa0101750	MtrunA17_Chr1g0154071	47.619	147	76	1	439	584	345	491	1.09e-34	137
Msa0101750	MtrunA17_Chr1g0154071	32.353	102	68	1	15	115	27	128	8.31e-11	64.7
Msa0101750	MtrunA17_Chr1g0154211	42.091	373	183	8	15	361	22	387	1.90e-83	266
Msa0101750	MtrunA17_Chr1g0154211	43.353	173	85	2	426	589	227	395	6.87e-38	144

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0101750	AT3G18990.2	25.405	370	229	13	13	361	3	346	1.91e-23	102
Msa0101750	AT3G18990.1	25.562	356	218	13	13	347	3	332	4.71e-23	101
Msa0101750	AT4G33280.3	23.932	351	227	13	15	336	24	363	7.14e-14	74.3
Msa0101750	AT5G66980.1	21.777	349	215	12	15	348	9	314	1.18e-13	72.8
Msa0101750	AT3G06220.1	26.712	146	98	2	14	158	8	145	2.65e-13	68.9
Msa0101750	AT5G42700.2	32.110	109	69	1	475	583	76	179	3.92e-12	65.9
Msa0101750	AT5G42700.2	30.556	144	89	3	216	354	42	179	5.02e-12	65.5
Msa0101750	AT3G06160.5	26.733	101	74	0	14	114	8	108	5.42e-12	67.8
Msa0101750	AT3G06160.4	26.733	101	74	0	14	114	8	108	5.42e-12	67.8
Msa0101750	AT3G06160.3	26.733	101	74	0	14	114	8	108	5.42e-12	67.8

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Find 139 sgRNAs with CRISPR-Local

Find 296 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGAGGACGTAAATGATT+GGG	0.490021	1_3:+8401628	None:intergenic
AAACAAAGACAGCTGTACCG+AGG	0.720792	1_3:-8399968	Msa0101750:intron
AAATCTATCCAATTATGAGT+TGG	0.532358	1_3:-8402030	Msa0101750:CDS
AAATTGGTTTCGTTAAGAAA+TGG	0.440445	1_3:-8403903	None:intergenic
AACTCTGTTCCAGATGCCAA+AGG	0.640736	1_3:-8399603	Msa0101750:CDS
AAGACCTTCCTTTCCAACCC+CGG	0.580500	1_3:+8399461	None:intergenic
AAGCTCCGCTGAGAATATCG+TGG	0.711691	1_3:+8401690	None:intergenic
AAGGTCTTGACCATCAAATT+GGG	0.499167	1_3:-8399446	Msa0101750:CDS
AATCATCTGTTCTTCCATCA+TGG	0.499739	1_3:-8402341	Msa0101750:CDS
AATCCTGTATAGAAGTGGGA+AGG	0.661650	1_3:-8401720	Msa0101750:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GCAGTAGTATATAAATTTCG+AGG	-	chr1_3:8399330-8399349	Msa0101750:three_prime_UTR	30.0%
CAAGTAAAATTGGAAGCAAA+TGG	-	chr1_3:8399685-8399704	Msa0101750:CDS	30.0%
AGTAAAATTGGAAGCAAATG+GGG	-	chr1_3:8399687-8399706	Msa0101750:CDS	30.0%
TCAAAAGCTAAATGTGTGTT+TGG	-	chr1_3:8399732-8399751	Msa0101750:intron	30.0%
TAAATGTGTGTTTGGATTGA+CGG	-	chr1_3:8399740-8399759	Msa0101750:intron	30.0%
AGAGTGATTGAAATGTTGTA+TGG	+	chr1_3:8400049-8400068	None:intergenic	30.0%
ATAGTTTCTCAGCTTCTTAT+AGG	+	chr1_3:8400211-8400230	None:intergenic	30.0%
ATACATGATACTGGAAATTG+AGG	+	chr1_3:8400367-8400386	None:intergenic	30.0%
CTTGTTTAGATACATGATAC+TGG	+	chr1_3:8400376-8400395	None:intergenic	30.0%
GGAGATCATCATTTACAATA+TGG	-	chr1_3:8400623-8400642	Msa0101750:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_3	gene	8399028	8403919	8399028	ID=Msa0101750;Name=Msa0101750
chr1_3	mRNA	8399028	8403919	8399028	ID=Msa0101750-mRNA-1;Parent=Msa0101750;Name=Msa0101750-mRNA-1;_AED=0.38;_eAED=0.38;_QI=296\|1\|1\|1\|0.71\|0.62\|8\|394\|596
chr1_3	exon	8399028	8399715	8399028	ID=Msa0101750-mRNA-1:exon:3367;Parent=Msa0101750-mRNA-1
chr1_3	exon	8399809	8399978	8399809	ID=Msa0101750-mRNA-1:exon:3366;Parent=Msa0101750-mRNA-1
chr1_3	exon	8400254	8400770	8400254	ID=Msa0101750-mRNA-1:exon:3365;Parent=Msa0101750-mRNA-1
chr1_3	exon	8401574	8401749	8401574	ID=Msa0101750-mRNA-1:exon:3364;Parent=Msa0101750-mRNA-1
chr1_3	exon	8401925	8402471	8401925	ID=Msa0101750-mRNA-1:exon:3363;Parent=Msa0101750-mRNA-1
chr1_3	exon	8403397	8403487	8403397	ID=Msa0101750-mRNA-1:exon:3362;Parent=Msa0101750-mRNA-1
chr1_3	exon	8403508	8403645	8403508	ID=Msa0101750-mRNA-1:exon:3361;Parent=Msa0101750-mRNA-1
chr1_3	exon	8403766	8403919	8403766	ID=Msa0101750-mRNA-1:exon:3360;Parent=Msa0101750-mRNA-1
chr1_3	five_prime_UTR	8403766	8403919	8403766	ID=Msa0101750-mRNA-1:five_prime_utr;Parent=Msa0101750-mRNA-1
chr1_3	five_prime_UTR	8403508	8403645	8403508	ID=Msa0101750-mRNA-1:five_prime_utr;Parent=Msa0101750-mRNA-1
chr1_3	five_prime_UTR	8403484	8403487	8403484	ID=Msa0101750-mRNA-1:five_prime_utr;Parent=Msa0101750-mRNA-1
chr1_3	CDS	8403397	8403483	8403397	ID=Msa0101750-mRNA-1:cds;Parent=Msa0101750-mRNA-1
chr1_3	CDS	8401925	8402471	8401925	ID=Msa0101750-mRNA-1:cds;Parent=Msa0101750-mRNA-1
chr1_3	CDS	8401574	8401749	8401574	ID=Msa0101750-mRNA-1:cds;Parent=Msa0101750-mRNA-1
chr1_3	CDS	8400254	8400770	8400254	ID=Msa0101750-mRNA-1:cds;Parent=Msa0101750-mRNA-1
chr1_3	CDS	8399809	8399978	8399809	ID=Msa0101750-mRNA-1:cds;Parent=Msa0101750-mRNA-1
chr1_3	CDS	8399422	8399715	8399422	ID=Msa0101750-mRNA-1:cds;Parent=Msa0101750-mRNA-1
chr1_3	three_prime_UTR	8399028	8399421	8399028	ID=Msa0101750-mRNA-1:three_prime_utr;Parent=Msa0101750-mRNA-1

Gene Sequence

>Msa0101750

ATGGGTCGTGAGTGTAAGGGAAAGTTAAATGTGAGAAAACCTCACTTCTATCACCTCTTTAACCCTTCTTCAACCTCTCAATCTCTGAGAGTTCCAGATGGTTTTGTCCATCAAATGGAGGGCACGACATGCGGATTAGTTTCATTAACTGGTCCTAGTGGAAATACTTGGCAAGTTCGATTAGTTGAGCAGGACAATCATCTGTTCTTCCATCATGGATGGTCAACATTTGTGGGAGATCATCATTTACAATATGGCGACCTTTTGGTTTTCAGATACGAGGGTCACTTGCATTTCACCGTGCAAGTATTTGGTGAGAATGCATGCGAGAAAGAAGCTGCATTTCATTCTGAGTGCAATCTAAATTCATTAAACTTCAATAATATTGAAGGACAGAAAAGTGATGCTGAGGTGATTTCTTCTTTGGATGTTGTTGGAGATGTTCCGAAGAAAATGAGGTGCGACGCCATCGAGAACCAGGAGCCTGAGTTGGCTATTGCTGGTAAAAATCTATCCAATTATGAGTTGGTTAGACCAATCAGTATGATCAGAGAAACAGAAGAAACCTGCAAAGAATGTTCAGCTAGTGCTGTCCATGTTCCTTTTCAAACAGGAAATAGTAATGAAGTTGAAGATGCAGCAATCCTGTATAGAAGTGGGAAGGAAGATGACCACGATATTCTCAGCGGAGCTTCACTATCAAAGTTATCAGCACATGATGAGAAAAAGATAGCCCAATCATTTACGTCCTCTTTTCCATATTTTGTTAAAATAATCAAAACCTTCAATGTCAGTGGTTCGTATACTCTGAATGTTCCATATCAATTTTCGATGTCACATTTTCCAAACTGCAAGACAAAGATTATTCTTTATAATTTGAAGGGAGAACACTGGACTGTTAATTCTGTTCCAACAACTAGAGTGCATACAAGTCACACTCTATGTGGAGGATGGATGGCTTTTGTTCGTGGCAATAGCATAAAAGTTGGAGATGTCTGCATTTTCGAACTTATACGTGAGTGTGAATTACGTGTTCGCATTGCTGAGGTTGGAAAAAATGGGTTAGACTGCCAGGTTGGAAAACTAGCTTGTAGCATGCTTAGTGAACGACATGATGTTGCTAGCCATAATACTTCCAGATATATGCCAAAGAAACCTAAAGTTAGCTCAAAGTGCAGAACGAAGGTTGATCTATCAGATAAAAAGTTGTCAAAGATTTGTCAAGAACCTGTTTTAGCCATTGAATTCAAGAAATCCGGTAGAGCTTCAACTACTTCGAAGAAGATGGGGCTTTCCCCCAAGTCAAAAGCTTCCCATAAAAAGACGGCTGTACCGAGGAAACATCGTGTTGAAGATGAACTAAGTTCCCAGGCTAAAGCTGGTTTGAGAATGTTGTTTGCACTCGACGAACAAAGAGTAGCTGAAGCTTTTACTTCTCCATTTCCTAATTTTGTGAAAATCATGAAGAAGTTCAATGTCAGTGGATCATACACTTTGAAAATCCCATATCAGTTCTCTGCTGCACATCTCCCCGCCTACAAAACAGAAGTTACGCTTCGCAATTCAAGAGGCCAGTGTTGGATTGTAAACTCTGTTCCAGATGCCAAAGGCAGAACAATTCACACCTTTTGTGGGGGATGGATAGCCTTTGTTCGTGATAATGACATCAATTTTGGCGATACTTGCATTTTCGAGCTCGTATCTCATTATGTGATGCAGGTTCATATATCCGGGGTTGGAAAGGAAGGTCTTGACCATCAAATTGGGCACGTGAAACTTAATAACATGTAG

Protein sequence

>Msa0101750

MGRECKGKLNVRKPHFYHLFNPSSTSQSLRVPDGFVHQMEGTTCGLVSLTGPSGNTWQVRLVEQDNHLFFHHGWSTFVGDHHLQYGDLLVFRYEGHLHFTVQVFGENACEKEAAFHSECNLNSLNFNNIEGQKSDAEVISSLDVVGDVPKKMRCDAIENQEPELAIAGKNLSNYELVRPISMIRETEETCKECSASAVHVPFQTGNSNEVEDAAILYRSGKEDDHDILSGASLSKLSAHDEKKIAQSFTSSFPYFVKIIKTFNVSGSYTLNVPYQFSMSHFPNCKTKIILYNLKGEHWTVNSVPTTRVHTSHTLCGGWMAFVRGNSIKVGDVCIFELIRECELRVRIAEVGKNGLDCQVGKLACSMLSERHDVASHNTSRYMPKKPKVSSKCRTKVDLSDKKLSKICQEPVLAIEFKKSGRASTTSKKMGLSPKSKASHKKTAVPRKHRVEDELSSQAKAGLRMLFALDEQRVAEAFTSPFPNFVKIMKKFNVSGSYTLKIPYQFSAAHLPAYKTEVTLRNSRGQCWIVNSVPDAKGRTIHTFCGGWIAFVRDNDINFGDTCIFELVSHYVMQVHISGVGKEGLDHQIGHVKLNNM*