AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0122550

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0122550 MtrunA17_Chr1g0182531 99.492 197 1 0 1 197 28 224 8.45e-145 401
Msa0122550 MtrunA17_Chr3g0077371 98.985 197 2 0 1 197 1 197 6.56e-144 398
Msa0122550 MtrunA17_Chr3g0077391 98.985 197 2 0 1 197 1 197 6.56e-144 398
Msa0122550 MtrunA17_Chr4g0009481 98.985 197 2 0 1 197 1 197 6.56e-144 398
Msa0122550 MtrunA17_Chr7g0241891 97.970 197 4 0 1 197 1 197 2.56e-142 394
Msa0122550 MtrunA17_Chr4g0060551 96.522 115 4 0 1 115 1 115 9.48e-77 225
Msa0122550 MtrunA17_Chr4g0009501 100.000 60 0 0 138 197 13 72 5.30e-38 125
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0122550 AT5G39850.1 90.909 187 17 0 1 187 1 187 4.21e-124 348
Msa0122550 AT5G15200.1 83.838 198 31 1 1 197 1 198 9.01e-117 330
Msa0122550 AT5G15200.2 88.321 137 16 0 1 137 1 137 3.84e-85 248

Find 87 sgRNAs with CRISPR-Local

Find 159 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAATACATTTGATGATCTT+TGG 0.199358 1_3:-49283030 Msa0122550:three_prime_UTR
ATCACCACCAGAAGCCTTCT+TGG 0.265320 1_3:+49283267 None:intergenic
TACAAACACCATTAAGTAAA+AGG 0.285041 1_3:+49282997 None:intergenic
CAATACATTTGATGATCTTT+GGG 0.308220 1_3:-49283029 Msa0122550:three_prime_UTR
CAGACACTTGTATTCAAATC+TGG 0.328033 1_3:-49284724 Msa0122550:CDS
AATAAACAAGGATAGGAAAA+TGG 0.330874 1_3:+49283132 None:intergenic
CCTTCTTGGCTGCAGCCTTA+AGG 0.354248 1_3:+49283281 None:intergenic
GTTTCATAGGGTTGGAAGGC+AGG 0.358634 1_3:-49283413 Msa0122550:intron
ATCATGCTAGGGTTCTTATC+AGG 0.370005 1_3:-49284684 Msa0122550:CDS
TCGAAAATCTACCTGTGTTC+CGG 0.383091 1_3:-49285213 Msa0122550:five_prime_UTR
CGATTCAACCATGCTTCATA+TGG 0.384231 1_3:-49282904 Msa0122550:three_prime_UTR
CAGAATTACAAGTTCTCAAA+AGG 0.386658 1_3:+49282870 None:intergenic
TCCTTATGAGAAGGAACGTT+TGG 0.386694 1_3:-49285001 Msa0122550:CDS
TTCGGTGCAAGAGGGAGCTT+TGG 0.394293 1_3:-49284945 Msa0122550:CDS
GCATGGTTGAATCGAAGTTT+TGG 0.398137 1_3:+49282913 None:intergenic
GCTGCAGCCAAGAAGGCTTC+TGG 0.408125 1_3:-49283274 Msa0122550:CDS
ACAGATTCCTTGCATTGTTA+CGG 0.425233 1_3:+49284896 None:intergenic
GGTGAACATCCCATCCTTCA+TGG 0.437604 1_3:-49283389 Msa0122550:CDS
CTTAAGCAAAACTCTATACT+TGG 0.438042 1_3:+49283060 None:intergenic
CAAAAGCCCAAAACAAGAAA+AGG 0.439712 1_3:-49285289 None:intergenic
TCCTTGCATTGTTACGGATA+CGG 0.449433 1_3:+49284902 None:intergenic
TTCTTTCGCTTGACGCGACC+AGG 0.452567 1_3:+49283304 None:intergenic
GGTGCAAGAGGGAGCTTTGG+AGG 0.455063 1_3:-49284942 Msa0122550:CDS
CAAAACAAGAAAAGGCCAAT+GGG 0.457636 1_3:-49285281 None:intergenic
AAGATAGATACTCACAGTTC+CGG 0.468521 1_3:+49285145 None:intergenic
AGTCTAGGACAGAGAACAAA+AGG 0.472560 1_3:+49283185 None:intergenic
GAATCCTCGTCGTATCTTTG+AGG 0.472904 1_3:-49284857 Msa0122550:CDS
TGGTTAAAGTTGCTTTCTAC+CGG 0.476762 1_3:-49285164 Msa0122550:CDS
TCCAAACGTTCCTTCTCATA+AGG 0.485854 1_3:+49285000 None:intergenic
GAACAAACTTGATTATGTCT+TGG 0.487846 1_3:-49284782 Msa0122550:CDS
TTGAAACTGGTGGGAGAGTA+CGG 0.490202 1_3:-49284970 Msa0122550:CDS
TTTGGATGCTGAGTTGAAAC+TGG 0.490439 1_3:-49284983 Msa0122550:CDS
CCTAGCATGATGAATGGACT+TGG 0.491509 1_3:+49284696 None:intergenic
TGGTGGGAGAGTACGGTCTT+CGG 0.497691 1_3:-49284963 Msa0122550:CDS
AACTTTAACCATCACGCCGC+CGG 0.508542 1_3:+49285176 None:intergenic
AATCCTCGTCGTATCTTTGA+GGG 0.510157 1_3:-49284856 Msa0122550:CDS
TGGGGTTTATAATAGAAAAC+CGG 0.516679 1_3:-49285262 Msa0122550:five_prime_UTR
CCTTAAGGCTGCAGCCAAGA+AGG 0.517095 1_3:-49283281 Msa0122550:CDS
TTCTGGTGGTGATGGTGATG+AGG 0.523720 1_3:-49283257 Msa0122550:CDS
AAGAACCCTAGCATGATGAA+TGG 0.528751 1_3:+49284690 None:intergenic
GCCGGTTCTCTACTCGCCGC+CGG 0.529489 1_3:+49285194 None:intergenic
TCAACCCTCACCATGAAGGA+TGG 0.531047 1_3:+49283379 None:intergenic
TCCGGCGGCGAGTAGAGAAC+CGG 0.531605 1_3:-49285195 Msa0122550:five_prime_UTR
CCAGGAAGACCACCACCAAA+AGG 0.535069 1_3:+49283322 None:intergenic
CCAAGTCCATTCATCATGCT+AGG 0.540233 1_3:-49284696 Msa0122550:CDS
ACTTGTATTCAAATCTGGTA+TGG 0.540887 1_3:-49284719 Msa0122550:CDS
CGTCAAGCGAAAGAACCTTA+AGG 0.541128 1_3:-49283296 Msa0122550:CDS
GATGCTAATTGTTTCACCAC+CGG 0.541799 1_3:+49285243 None:intergenic
CTCGCATAATAAACAAGGAT+AGG 0.549068 1_3:+49283125 None:intergenic
TTTGGTTTCATAGGGTTGGA+AGG 0.553079 1_3:-49283417 Msa0122550:intron
TACAAGTTCTCAAAAGGCAC+AGG 0.553498 1_3:+49282876 None:intergenic
GTGCAAGAGGGAGCTTTGGA+GGG 0.553805 1_3:-49284941 Msa0122550:CDS
TGATAGAAAATCTAAAGTCT+AGG 0.553926 1_3:+49283170 None:intergenic
TGCAAGGAATCTGTTAACAT+TGG 0.555760 1_3:-49284887 Msa0122550:CDS
CTATCCTTGTTTATTATGCG+AGG 0.560772 1_3:-49283124 Msa0122550:three_prime_UTR
CCAAAACAAGAAAAGGCCAA+TGG 0.567658 1_3:-49285282 None:intergenic
GTAGAGAACCGGCGGCGTGA+TGG 0.569580 1_3:-49285184 Msa0122550:exon
CAACCCTCACCATGAAGGAT+GGG 0.569875 1_3:+49283380 None:intergenic
TGAATCAACCCTCACCATGA+AGG 0.571223 1_3:+49283375 None:intergenic
TTATCAGGCAGAGACACATC+AGG 0.576233 1_3:-49284669 Msa0122550:intron
TCTACTCGCCGCCGGAACAC+AGG 0.579171 1_3:+49285202 None:intergenic
AAGAAGGCTTCTGGTGGTGA+TGG 0.583588 1_3:-49283265 Msa0122550:CDS
AGTACGGTCTTCGGTGCAAG+AGG 0.589539 1_3:-49284954 Msa0122550:CDS
CAAGTCCATTCATCATGCTA+GGG 0.590386 1_3:-49284695 Msa0122550:CDS
GCCGCGTCGTCCTTATGAGA+AGG 0.591592 1_3:-49285010 Msa0122550:CDS
CATCCCATCCTTCATGGTGA+GGG 0.593258 1_3:-49283383 Msa0122550:CDS
GCCGTATCCGTAACAATGCA+AGG 0.595109 1_3:-49284903 Msa0122550:CDS
AGGGTGAAGCACTATTGAGA+AGG 0.597313 1_3:-49284837 Msa0122550:CDS
TCATAGGGTTGGAAGGCAGG+TGG 0.599698 1_3:-49283410 Msa0122550:intron
GCAGCCAAGAAGGCTTCTGG+TGG 0.599775 1_3:-49283271 Msa0122550:CDS
GGATGCTGAGTTGAAACTGG+TGG 0.607337 1_3:-49284980 Msa0122550:CDS
ACATCCCATCCTTCATGGTG+AGG 0.616704 1_3:-49283384 Msa0122550:CDS
TGGTGGTGATGGTGATGAGG+AGG 0.622606 1_3:-49283254 Msa0122550:CDS
TTGAGAAGGATGTTTAAGCA+TGG 0.626035 1_3:-49284823 Msa0122550:CDS
GAGAACAAAAGGCTAGAGCA+AGG 0.632584 1_3:+49283196 None:intergenic
ATTTCCTCGCATAATAAACA+AGG 0.635314 1_3:+49283120 None:intergenic
AAAATCTACCTGTGTTCCGG+CGG 0.637056 1_3:-49285210 Msa0122550:five_prime_UTR
GATGCTGAGTTGAAACTGGT+GGG 0.650447 1_3:-49284979 Msa0122550:CDS
ATTTGAATACAAGTGTCTGA+AGG 0.654254 1_3:+49284728 None:intergenic
AGGCTACACCATATGAAGCA+TGG 0.656502 1_3:+49282896 None:intergenic
GGCGGCGAGTAGAGAACCGG+CGG 0.659354 1_3:-49285192 Msa0122550:five_prime_UTR
TGAATACAAGTGTCTGAAGG+CGG 0.659618 1_3:+49284731 None:intergenic
AAAACAAGAAAAGGCCAATG+GGG 0.679374 1_3:-49285280 None:intergenic
GTACGGTCTTCGGTGCAAGA+GGG 0.685910 1_3:-49284953 Msa0122550:CDS
GGTTTATAATAGAAAACCGG+TGG 0.690776 1_3:-49285259 Msa0122550:five_prime_UTR
TCCTTCTCATAAGGACGACG+CGG 0.741698 1_3:+49285009 None:intergenic
TCACCCTCAAAGATACGACG+AGG 0.762301 1_3:+49284853 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTAATGATAATAATGAAA+TGG + chr1_3:49282984-49283003 None:intergenic 10.0%
!!! TGAATTTTTCTTATTTTTTA+TGG - chr1_3:49283500-49283519 Msa0122550:intron 10.0%
!! AAATATGAAAAATATAAACA+AGG + chr1_3:49284581-49284600 None:intergenic 10.0%
!! AATAAATAAATAAACATTAG+GGG + chr1_3:49284619-49284638 None:intergenic 10.0%
!!! ATGTATGATAATATTATTTT+TGG - chr1_3:49284668-49284687 Msa0122550:intron 10.0%
!! ATATAATTTAGTGTAATCAT+AGG + chr1_3:49284007-49284026 None:intergenic 15.0%
!!! ATCATTTTCTCAAATATTAT+CGG - chr1_3:49284025-49284044 Msa0122550:intron 15.0%
!!! AATATTATTTTTGGTTTCAT+AGG - chr1_3:49284677-49284696 Msa0122550:CDS 15.0%
!!! ATATTATTTTTGGTTTCATA+GGG - chr1_3:49284678-49284697 Msa0122550:CDS 15.0%
!!! ACAATTGTTTGTTGAATTTA+TGG - chr1_3:49283022-49283041 Msa0122550:three_prime_UTR 20.0%
!!! TTCTCATTTGCTATAAAAAA+TGG + chr1_3:49283553-49283572 None:intergenic 20.0%
!!! TGTTTTTTCAAAATAATGCA+GGG + chr1_3:49283749-49283768 None:intergenic 20.0%
!!! ATGTTTTTTCAAAATAATGC+AGG + chr1_3:49283750-49283769 None:intergenic 20.0%
!! CAAAACATGTATAGAATTAA+AGG + chr1_3:49284210-49284229 None:intergenic 20.0%
!!! ATTTTTTTTGTTACTCTGAA+TGG - chr1_3:49284553-49284572 Msa0122550:intron 20.0%
!!! TTCATATTTTGTATGCTTAT+TGG - chr1_3:49284592-49284611 Msa0122550:intron 20.0%
!!! TAATTGAACCTTTTACTTAA+TGG - chr1_3:49285098-49285117 Msa0122550:intron 20.0%
!!! TTTGATTTTGCATATTTTGA+TGG - chr1_3:49285144-49285163 Msa0122550:intron 20.0%
!!! TTGATTTTGCATATTTTGAT+GGG - chr1_3:49285145-49285164 Msa0122550:intron 20.0%
!! TTTTCTATTATAAACCCCAT+TGG + chr1_3:49282840-49282859 None:intergenic 25.0%
!!! AACTTGCATTTTTTTTCTAG+CGG - chr1_3:49284317-49284336 Msa0122550:intron 25.0%
!! TAGTCTTAACGAAATATGTT+TGG - chr1_3:49284643-49284662 Msa0122550:intron 25.0%
! AGTCTTAACGAAATATGTTT+GGG - chr1_3:49284644-49284663 Msa0122550:intron 25.0%
!! TGATAGAAAATCTAAAGTCT+AGG + chr1_3:49284936-49284955 None:intergenic 25.0%
! AATAAACAAGGATAGGAAAA+TGG + chr1_3:49284974-49284993 None:intergenic 25.0%
!! TCAATACATTTGATGATCTT+TGG - chr1_3:49285073-49285092 Msa0122550:intron 25.0%
!! CAATACATTTGATGATCTTT+GGG - chr1_3:49285074-49285093 Msa0122550:intron 25.0%
! TACAAACACCATTAAGTAAA+AGG + chr1_3:49285109-49285128 None:intergenic 25.0%
!!! TGATTTTGCATATTTTGATG+GGG - chr1_3:49285146-49285165 Msa0122550:intron 25.0%
TGGGGTTTATAATAGAAAAC+CGG - chr1_3:49282841-49282860 Msa0122550:three_prime_UTR 30.0%
TTGAATTGAATACACACAGA+TGG - chr1_3:49283055-49283074 Msa0122550:three_prime_UTR 30.0%
TGAATTGAATACACACAGAT+GGG - chr1_3:49283056-49283075 Msa0122550:three_prime_UTR 30.0%
GAACAAACTTGATTATGTCT+TGG - chr1_3:49283321-49283340 Msa0122550:CDS 30.0%
ATTTGAATACAAGTGTCTGA+AGG + chr1_3:49283378-49283397 None:intergenic 30.0%
ACTTGTATTCAAATCTGGTA+TGG - chr1_3:49283384-49283403 Msa0122550:CDS 30.0%
!! GCTTATTTGAGTTTGTCTTT+TGG + chr1_3:49283713-49283732 None:intergenic 30.0%
GTAGATCATGTTTAGATCAT+TGG - chr1_3:49283848-49283867 Msa0122550:intron 30.0%
!! GATTATGTTTTTCTTGGTGT+CGG - chr1_3:49283959-49283978 Msa0122550:intron 30.0%
TTCTCAAATATTATCGGTCT+CGG - chr1_3:49284031-49284050 Msa0122550:intron 30.0%
TGTTTGTATTCACCAAGAAT+TGG - chr1_3:49284124-49284143 Msa0122550:intron 30.0%
!!! TTCCTGTTTTTTCCAATTCT+TGG + chr1_3:49284139-49284158 None:intergenic 30.0%
! ACAGGAAAGTATATGTGAAA+TGG - chr1_3:49284152-49284171 Msa0122550:intron 30.0%
! CAGGAAAGTATATGTGAAAT+GGG - chr1_3:49284153-49284172 Msa0122550:intron 30.0%
! AGGAAAGTATATGTGAAATG+GGG - chr1_3:49284154-49284173 Msa0122550:intron 30.0%
CAATATGTTCTATGTATCGA+AGG - chr1_3:49284359-49284378 Msa0122550:intron 30.0%
AACAGACAAGATCATATTAC+AGG + chr1_3:49284423-49284442 None:intergenic 30.0%
!!! TACTCAAATGTCACTGATTT+TGG - chr1_3:49284452-49284471 Msa0122550:intron 30.0%
!!! TATTTTTGGTTTCATAGGGT+TGG - chr1_3:49284682-49284701 Msa0122550:CDS 30.0%
ATTTCCTCGCATAATAAACA+AGG + chr1_3:49284986-49285005 None:intergenic 30.0%
CTTAAGCAAAACTCTATACT+TGG + chr1_3:49285046-49285065 None:intergenic 30.0%
CAGAATTACAAGTTCTCAAA+AGG + chr1_3:49285236-49285255 None:intergenic 30.0%
GGTTTATAATAGAAAACCGG+TGG - chr1_3:49282844-49282863 Msa0122550:three_prime_UTR 35.0%
! TGGTTAAAGTTGCTTTCTAC+CGG - chr1_3:49282939-49282958 Msa0122550:three_prime_UTR 35.0%
AAGATAGATACTCACAGTTC+CGG + chr1_3:49282961-49282980 None:intergenic 35.0%
ACAGATTCCTTGCATTGTTA+CGG + chr1_3:49283210-49283229 None:intergenic 35.0%
TGCAAGGAATCTGTTAACAT+TGG - chr1_3:49283216-49283235 Msa0122550:three_prime_UTR 35.0%
TTGAGAAGGATGTTTAAGCA+TGG - chr1_3:49283280-49283299 Msa0122550:CDS 35.0%
CAGACACTTGTATTCAAATC+TGG - chr1_3:49283379-49283398 Msa0122550:CDS 35.0%
TCAAATAAGCCAATCCAAAC+AGG - chr1_3:49283723-49283742 Msa0122550:intron 35.0%
TGGTTGTCTCTAACAATGTT+TGG - chr1_3:49283868-49283887 Msa0122550:intron 35.0%
!! GTTTGGTTTGCATTGTTGTA+TGG - chr1_3:49283885-49283904 Msa0122550:intron 35.0%
! CTCCTGGATTATGTTTTTCT+TGG - chr1_3:49283953-49283972 Msa0122550:intron 35.0%
CACCAAGAAAAACATAATCC+AGG + chr1_3:49283958-49283977 None:intergenic 35.0%
CACCAAGAATTGGAAAAAAC+AGG - chr1_3:49284134-49284153 Msa0122550:intron 35.0%
!! TTCAAGTTGCCAGCAAAAAT+GGG + chr1_3:49284246-49284265 None:intergenic 35.0%
!!! TTTTTTTCTAGCGGCTGTTA+TGG - chr1_3:49284326-49284345 Msa0122550:intron 35.0%
TTCTATGTATCGAAGGTAGT+AGG - chr1_3:49284366-49284385 Msa0122550:intron 35.0%
! TCCATTGTTCAAGTAATGGA+GGG - chr1_3:49284395-49284414 Msa0122550:intron 35.0%
! TTTTCACTCACAAGTCCTTT+TGG - chr1_3:49284766-49284785 Msa0122550:CDS 35.0%
CTCGCATAATAAACAAGGAT+AGG + chr1_3:49284981-49285000 None:intergenic 35.0%
! CTATCCTTGTTTATTATGCG+AGG - chr1_3:49284979-49284998 Msa0122550:CDS 35.0%
GATGCTAATTGTTTCACCAC+CGG + chr1_3:49282863-49282882 None:intergenic 40.0%
! CACAGGTAGATTTTCGAAGA+AGG + chr1_3:49282887-49282906 None:intergenic 40.0%
TCGAAAATCTACCTGTGTTC+CGG - chr1_3:49282890-49282909 Msa0122550:three_prime_UTR 40.0%
TCCTTATGAGAAGGAACGTT+TGG - chr1_3:49283102-49283121 Msa0122550:three_prime_UTR 40.0%
TCCAAACGTTCCTTCTCATA+AGG + chr1_3:49283106-49283125 None:intergenic 40.0%
! TTTGGATGCTGAGTTGAAAC+TGG - chr1_3:49283120-49283139 Msa0122550:three_prime_UTR 40.0%
TCCTTGCATTGTTACGGATA+CGG + chr1_3:49283204-49283223 None:intergenic 40.0%
AATCCTCGTCGTATCTTTGA+GGG - chr1_3:49283247-49283266 Msa0122550:CDS 40.0%
TGAATACAAGTGTCTGAAGG+CGG + chr1_3:49283375-49283394 None:intergenic 40.0%
CAAGTCCATTCATCATGCTA+GGG - chr1_3:49283408-49283427 Msa0122550:intron 40.0%
AAGAACCCTAGCATGATGAA+TGG + chr1_3:49283416-49283435 None:intergenic 40.0%
ATCATGCTAGGGTTCTTATC+AGG - chr1_3:49283419-49283438 Msa0122550:intron 40.0%
AAAATAATGCAGGGCCTGTT+TGG + chr1_3:49283740-49283759 None:intergenic 40.0%
! TACTCGCTTTCTGTGTTTTG+TGG - chr1_3:49283910-49283929 Msa0122550:intron 40.0%
! ACTCGCTTTCTGTGTTTTGT+GGG - chr1_3:49283911-49283930 Msa0122550:intron 40.0%
ATATGTGAAATGGGGTACAC+AGG - chr1_3:49284162-49284181 Msa0122550:intron 40.0%
! TCAAGTTGCCAGCAAAAATG+GGG + chr1_3:49284245-49284264 None:intergenic 40.0%
!! GTTCAAGTTGCCAGCAAAAA+TGG + chr1_3:49284247-49284266 None:intergenic 40.0%
CCATTACTTGAACAATGGAG+AGG + chr1_3:49284394-49284413 None:intergenic 40.0%
CCTCTCCATTGTTCAAGTAA+TGG - chr1_3:49284391-49284410 Msa0122550:intron 40.0%
CTCCATTGTTCAAGTAATGG+AGG - chr1_3:49284394-49284413 Msa0122550:intron 40.0%
GTGAATGAAGAGAAGAGAGA+TGG - chr1_3:49284519-49284538 Msa0122550:intron 40.0%
! TTTGGTTTCATAGGGTTGGA+AGG - chr1_3:49284686-49284705 Msa0122550:CDS 40.0%
! AGTCTAGGACAGAGAACAAA+AGG + chr1_3:49284921-49284940 None:intergenic 40.0%
!! TTTTGGTTGGTTGGATACAC+AGG + chr1_3:49285176-49285195 None:intergenic 40.0%
!!! GAATCGAAGTTTTGGTTGGT+TGG + chr1_3:49285185-49285204 None:intergenic 40.0%
!! GGTTGAATCGAAGTTTTGGT+TGG + chr1_3:49285189-49285208 None:intergenic 40.0%
! GCATGGTTGAATCGAAGTTT+TGG + chr1_3:49285193-49285212 None:intergenic 40.0%
CGATTCAACCATGCTTCATA+TGG - chr1_3:49285199-49285218 Msa0122550:five_prime_UTR 40.0%
! TACAAGTTCTCAAAAGGCAC+AGG + chr1_3:49285230-49285249 None:intergenic 40.0%
AAAATCTACCTGTGTTCCGG+CGG - chr1_3:49282893-49282912 Msa0122550:three_prime_UTR 45.0%
! GATGCTGAGTTGAAACTGGT+GGG - chr1_3:49283124-49283143 Msa0122550:three_prime_UTR 45.0%
TTGAAACTGGTGGGAGAGTA+CGG - chr1_3:49283133-49283152 Msa0122550:three_prime_UTR 45.0%
GAATCCTCGTCGTATCTTTG+AGG - chr1_3:49283246-49283265 Msa0122550:CDS 45.0%
! AGGGTGAAGCACTATTGAGA+AGG - chr1_3:49283266-49283285 Msa0122550:CDS 45.0%
! CCTAGCATGATGAATGGACT+TGG + chr1_3:49283410-49283429 None:intergenic 45.0%
CCAAGTCCATTCATCATGCT+AGG - chr1_3:49283407-49283426 Msa0122550:CDS 45.0%
TTATCAGGCAGAGACACATC+AGG - chr1_3:49283434-49283453 Msa0122550:intron 45.0%
TGTCCATGTCTTTGCGTCAT+AGG - chr1_3:49284061-49284080 Msa0122550:intron 45.0%
!!! GACTAAAGCTGCTGCTTTTC+TGG - chr1_3:49284271-49284290 Msa0122550:intron 45.0%
GCCCTCCATTACTTGAACAA+TGG + chr1_3:49284399-49284418 None:intergenic 45.0%
TGAATCAACCCTCACCATGA+AGG + chr1_3:49284731-49284750 None:intergenic 45.0%
! TCACTCACAAGTCCTTTTGG+TGG - chr1_3:49284769-49284788 Msa0122550:CDS 45.0%
CGTCAAGCGAAAGAACCTTA+AGG - chr1_3:49284807-49284826 Msa0122550:CDS 45.0%
GAGAACAAAAGGCTAGAGCA+AGG + chr1_3:49284910-49284929 None:intergenic 45.0%
AGGCTACACCATATGAAGCA+TGG + chr1_3:49285210-49285229 None:intergenic 45.0%
!! AAATAAATAAATAAACATTA+GGG + chr1_3:49284620-49284639 None:intergenic 5.0%
!! TAAATAAATAAATAAACATT+AGG + chr1_3:49284621-49284640 None:intergenic 5.0%
AACTTTAACCATCACGCCGC+CGG + chr1_3:49282930-49282949 None:intergenic 50.0%
TCCTTCTCATAAGGACGACG+CGG + chr1_3:49283097-49283116 None:intergenic 50.0%
! GGATGCTGAGTTGAAACTGG+TGG - chr1_3:49283123-49283142 Msa0122550:three_prime_UTR 50.0%
GCCGTATCCGTAACAATGCA+AGG - chr1_3:49283200-49283219 Msa0122550:three_prime_UTR 50.0%
TCACCCTCAAAGATACGACG+AGG + chr1_3:49283253-49283272 None:intergenic 50.0%
AATGCAGGGCCTGTTTGGAT+TGG + chr1_3:49283735-49283754 None:intergenic 50.0%
CTATGAAGCACGAACACTCC+TGG - chr1_3:49283937-49283956 Msa0122550:intron 50.0%
GCACCTATGACGCAAAGACA+TGG + chr1_3:49284067-49284086 None:intergenic 50.0%
!! TGCTACTGCCCCATTTTTGC+TGG - chr1_3:49284234-49284253 Msa0122550:intron 50.0%
GTTTCATAGGGTTGGAAGGC+AGG - chr1_3:49284690-49284709 Msa0122550:CDS 50.0%
GGTGAACATCCCATCCTTCA+TGG - chr1_3:49284714-49284733 Msa0122550:CDS 50.0%
ACATCCCATCCTTCATGGTG+AGG - chr1_3:49284719-49284738 Msa0122550:CDS 50.0%
CATCCCATCCTTCATGGTGA+GGG - chr1_3:49284720-49284739 Msa0122550:CDS 50.0%
CAACCCTCACCATGAAGGAT+GGG + chr1_3:49284726-49284745 None:intergenic 50.0%
TCAACCCTCACCATGAAGGA+TGG + chr1_3:49284727-49284746 None:intergenic 50.0%
! CTCACAAGTCCTTTTGGTGG+TGG - chr1_3:49284772-49284791 Msa0122550:CDS 50.0%
ATCACCACCAGAAGCCTTCT+TGG + chr1_3:49284839-49284858 None:intergenic 50.0%
! AAGAAGGCTTCTGGTGGTGA+TGG - chr1_3:49284838-49284857 Msa0122550:CDS 50.0%
!! TTCTGGTGGTGATGGTGATG+AGG - chr1_3:49284846-49284865 Msa0122550:CDS 50.0%
! TGGTGGGAGAGTACGGTCTT+CGG - chr1_3:49283140-49283159 Msa0122550:three_prime_UTR 55.0%
! AGTACGGTCTTCGGTGCAAG+AGG - chr1_3:49283149-49283168 Msa0122550:three_prime_UTR 55.0%
! GTACGGTCTTCGGTGCAAGA+GGG - chr1_3:49283150-49283169 Msa0122550:three_prime_UTR 55.0%
! TTCGGTGCAAGAGGGAGCTT+TGG - chr1_3:49283158-49283177 Msa0122550:three_prime_UTR 55.0%
!! GTGCAAGAGGGAGCTTTGGA+GGG - chr1_3:49283162-49283181 Msa0122550:three_prime_UTR 55.0%
TCATAGGGTTGGAAGGCAGG+TGG - chr1_3:49284693-49284712 Msa0122550:CDS 55.0%
CCAGGAAGACCACCACCAAA+AGG + chr1_3:49284784-49284803 None:intergenic 55.0%
!!! CCTTTTGGTGGTGGTCTTCC+TGG - chr1_3:49284781-49284800 Msa0122550:CDS 55.0%
TTCTTTCGCTTGACGCGACC+AGG + chr1_3:49284802-49284821 None:intergenic 55.0%
CCTTCTTGGCTGCAGCCTTA+AGG + chr1_3:49284825-49284844 None:intergenic 55.0%
CCTTAAGGCTGCAGCCAAGA+AGG - chr1_3:49284822-49284841 Msa0122550:CDS 55.0%
!! TGGTGGTGATGGTGATGAGG+AGG - chr1_3:49284849-49284868 Msa0122550:CDS 55.0%
GCCGCGTCGTCCTTATGAGA+AGG - chr1_3:49283093-49283112 Msa0122550:three_prime_UTR 60.0%
! GGTGCAAGAGGGAGCTTTGG+AGG - chr1_3:49283161-49283180 Msa0122550:three_prime_UTR 60.0%
GCTGCAGCCAAGAAGGCTTC+TGG - chr1_3:49284829-49284848 Msa0122550:CDS 60.0%
GCAGCCAAGAAGGCTTCTGG+TGG - chr1_3:49284832-49284851 Msa0122550:CDS 60.0%
TCTACTCGCCGCCGGAACAC+AGG + chr1_3:49282904-49282923 None:intergenic 65.0%
TCCGGCGGCGAGTAGAGAAC+CGG - chr1_3:49282908-49282927 Msa0122550:three_prime_UTR 65.0%
GTAGAGAACCGGCGGCGTGA+TGG - chr1_3:49282919-49282938 Msa0122550:three_prime_UTR 65.0%
! GCCGGTTCTCTACTCGCCGC+CGG + chr1_3:49282912-49282931 None:intergenic 70.0%
GGCGGCGAGTAGAGAACCGG+CGG - chr1_3:49282911-49282930 Msa0122550:three_prime_UTR 70.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 49282827 49285298 49282827 ID=Msa0122550;Name=Msa0122550
chr1_3 mRNA 49282827 49285298 49282827 ID=Msa0122550-mRNA-1;Parent=Msa0122550;Name=Msa0122550-mRNA-1;_AED=0.05;_eAED=0.05;_QI=111|1|1|1|1|1|3|416|197
chr1_3 exon 49282827 49283426 49282827 ID=Msa0122550-mRNA-1:exon:14897;Parent=Msa0122550-mRNA-1
chr1_3 exon 49284670 49285051 49284670 ID=Msa0122550-mRNA-1:exon:14896;Parent=Msa0122550-mRNA-1
chr1_3 exon 49285160 49285298 49285160 ID=Msa0122550-mRNA-1:exon:14895;Parent=Msa0122550-mRNA-1
chr1_3 five_prime_UTR 49285188 49285298 49285188 ID=Msa0122550-mRNA-1:five_prime_utr;Parent=Msa0122550-mRNA-1
chr1_3 CDS 49285160 49285187 49285160 ID=Msa0122550-mRNA-1:cds;Parent=Msa0122550-mRNA-1
chr1_3 CDS 49284670 49285051 49284670 ID=Msa0122550-mRNA-1:cds;Parent=Msa0122550-mRNA-1
chr1_3 CDS 49283243 49283426 49283243 ID=Msa0122550-mRNA-1:cds;Parent=Msa0122550-mRNA-1
chr1_3 three_prime_UTR 49282827 49283242 49282827 ID=Msa0122550-mRNA-1:three_prime_utr;Parent=Msa0122550-mRNA-1
Gene Sequence

>Msa0122550

ATGGTTAAAGTTGCTTTCTACCGGAACTATGGGAAAACATTCAAAAAGCCGCGTCGTCCTTATGAGAAGGAACGTTTGGATGCTGAGTTGAAACTGGTGGGAGAGTACGGTCTTCGGTGCAAGAGGGAGCTTTGGAGGGTTCAGTATGCTCTGAGCCGTATCCGTAACAATGCAAGGAATCTGTTAACATTGGATGAGAAGAATCCTCGTCGTATCTTTGAGGGTGAAGCACTATTGAGAAGGATGTTTAAGCATGGTCTTCTTGATGAAACTCAGAACAAACTTGATTATGTCTTGGCTCTCACTGTTGAGAATTTTCTTGAACGCCGCCTTCAGACACTTGTATTCAAATCTGGTATGGCCAAGTCCATTCATCATGCTAGGGTTCTTATCAGGCAGAGACACATCAGGGTTGGAAGGCAGGTGGTGAACATCCCATCCTTCATGGTGAGGGTTGATTCACAGAAGCACATTGACTTTTCACTCACAAGTCCTTTTGGTGGTGGTCTTCCTGGTCGCGTCAAGCGAAAGAACCTTAAGGCTGCAGCCAAGAAGGCTTCTGGTGGTGATGGTGATGAGGAGGATGAAGATTAA
Protein sequence

>Msa0122550

MVKVAFYRNYGKTFKKPRRPYEKERLDAELKLVGEYGLRCKRELWRVQYALSRIRNNARNLLTLDEKNPRRIFEGEALLRRMFKHGLLDETQNKLDYVLALTVENFLERRLQTLVFKSGMAKSIHHARVLIRQRHIRVGRQVVNIPSFMVRVDSQKHIDFSLTSPFGGGLPGRVKRKNLKAAAKKASGGDGDEEDED*