Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0191260 | AFK42939.1 | 94.667 | 75 | 4 | 0 | 1 | 75 | 15 | 89 | 3.22e-44 | 147 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0191260 | I3SRP7 | 94.667 | 75 | 4 | 0 | 1 | 75 | 15 | 89 | 1.54e-44 | 147 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0029700 | Msa0191260 | 0.812636 | 3.836386e-51 | -8.615850e-47 |
| Msa0080020 | Msa0191260 | 0.821400 | 4.120706e-53 | -8.615850e-47 |
| Msa0110240 | Msa0191260 | 0.813269 | 2.788535e-51 | -8.615850e-47 |
| Msa0124960 | Msa0191260 | 0.816256 | 6.073191e-52 | -8.615850e-47 |
| Msa0154730 | Msa0191260 | 0.821969 | 3.043357e-53 | -8.615850e-47 |
| Msa0191260 | Msa0311900 | 0.954006 | 7.791986e-112 | -8.615850e-47 |
| Msa0191260 | Msa0316140 | 0.800934 | 1.141682e-48 | -8.615850e-47 |
| Msa0191260 | Msa0511380 | 0.808515 | 2.981974e-50 | -8.615850e-47 |
| Msa0191260 | Msa0700410 | 0.807564 | 4.751828e-50 | -8.615850e-47 |
| Msa0191260 | Msa0829580 | 0.921612 | 2.862428e-88 | -8.615850e-47 |
| Msa0191260 | Msa1026970 | 0.840931 | 6.458333e-58 | -8.615850e-47 |
| Msa0191260 | Msa1111900 | 0.816238 | 6.129316e-52 | -8.615850e-47 |
| Msa0191260 | Msa1133760 | 0.841577 | 4.367883e-58 | -8.615850e-47 |
| Msa0191260 | Msa1154310 | 0.828340 | 9.491958e-55 | -8.615850e-47 |
| Msa0191260 | Msa1214780 | 0.803563 | 3.283831e-49 | -8.615850e-47 |
| Msa0191260 | Msa1300730 | 0.807200 | 5.676050e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0191260 | MtrunA17_Chr2g0281091 | 94.667 | 75 | 4 | 0 | 1 | 75 | 15 | 89 | 2.96e-48 | 147 |
| Msa0191260 | MtrunA17_Chr2g0323571 | 42.105 | 76 | 38 | 3 | 3 | 75 | 19 | 91 | 1.00e-11 | 55.1 |
| Msa0191260 | MtrunA17_Chr2g0323671 | 42.105 | 76 | 38 | 3 | 3 | 75 | 19 | 91 | 1.99e-11 | 54.3 |
| Msa0191260 | MtrunA17_Chr2g0323631 | 42.105 | 76 | 38 | 3 | 3 | 75 | 19 | 91 | 2.95e-11 | 53.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 12 sgRNAs with CRISPR-Local
Find 9 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATGGATTTCTTAATCAATT+TGG | 0.148938 | 2_1:+6814882 | None:intergenic |
| AACTGCAATGAGAACGCTTC+AGG | 0.302945 | 2_1:-6815108 | None:intergenic |
| CTGTCATGTACTTGAGCTGC+TGG | 0.406277 | 2_1:-6814908 | Msa0191260:CDS |
| AACTTCTTCAAAGCAAGTTA+AGG | 0.411883 | 2_1:-6815030 | Msa0191260:CDS |
| CTTCAAAGCAAGTTAAGGCT+AGG | 0.439718 | 2_1:-6815025 | Msa0191260:CDS |
| TGTCATGTACTTGAGCTGCT+GGG | 0.470465 | 2_1:-6814907 | Msa0191260:CDS |
| AATTCATCTTCTACTTTCAA+AGG | 0.482157 | 2_1:-6814975 | Msa0191260:CDS |
| TTCAAAGCAAGTTAAGGCTA+GGG | 0.484226 | 2_1:-6815024 | Msa0191260:CDS |
| ACTGCAATGAGAACGCTTCA+GGG | 0.509342 | 2_1:-6815107 | None:intergenic |
| AACGTGCTGAAGAGTCTCTT+AGG | 0.527974 | 2_1:-6814932 | Msa0191260:CDS |
| GTCATGTACTTGAGCTGCTG+GGG | 0.565171 | 2_1:-6814906 | Msa0191260:CDS |
| CAAAGGTAGCAACAGTGACA+AGG | 0.606257 | 2_1:-6814958 | Msa0191260:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AATTCATCTTCTACTTTCAA+AGG | - | chr2_1:6815022-6815041 | Msa0191260:CDS | 25.0% |
| AACTTCTTCAAAGCAAGTTA+AGG | - | chr2_1:6814967-6814986 | Msa0191260:CDS | 30.0% | |
| TTCAAAGCAAGTTAAGGCTA+GGG | - | chr2_1:6814973-6814992 | Msa0191260:CDS | 35.0% | |
| CTTCAAAGCAAGTTAAGGCT+AGG | - | chr2_1:6814972-6814991 | Msa0191260:CDS | 40.0% | |
| CAAAGGTAGCAACAGTGACA+AGG | - | chr2_1:6815039-6815058 | Msa0191260:CDS | 45.0% | |
| AACGTGCTGAAGAGTCTCTT+AGG | - | chr2_1:6815065-6815084 | Msa0191260:CDS | 45.0% | |
| TGTCATGTACTTGAGCTGCT+GGG | - | chr2_1:6815090-6815109 | Msa0191260:CDS | 45.0% | |
| CTGTCATGTACTTGAGCTGC+TGG | - | chr2_1:6815089-6815108 | Msa0191260:CDS | 50.0% | |
| GTCATGTACTTGAGCTGCTG+GGG | - | chr2_1:6815091-6815110 | Msa0191260:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_1 | gene | 6814896 | 6815123 | 6814896 | ID=Msa0191260;Name=Msa0191260 |
| chr2_1 | mRNA | 6814896 | 6815123 | 6814896 | ID=Msa0191260-mRNA-1;Parent=Msa0191260;Name=Msa0191260-mRNA-1;_AED=0.09;_eAED=0.09;_QI=0|-1|0|1|-1|1|1|0|75 |
| chr2_1 | exon | 6814896 | 6815123 | 6814896 | ID=Msa0191260-mRNA-1:exon:3109;Parent=Msa0191260-mRNA-1 |
| chr2_1 | CDS | 6814896 | 6815123 | 6814896 | ID=Msa0191260-mRNA-1:cds;Parent=Msa0191260-mRNA-1 |
| Gene Sequence |
| Protein sequence |
