Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0311900 | AFK42939.1 | 94.667 | 75 | 4 | 0 | 1 | 75 | 15 | 89 | 3.22e-44 | 147 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0029700 | Msa0311900 | 0.808080 | 3.692569e-50 | -8.615850e-47 |
| Msa0080020 | Msa0311900 | 0.817040 | 4.052779e-52 | -8.615850e-47 |
| Msa0110240 | Msa0311900 | 0.800030 | 1.744828e-48 | -8.615850e-47 |
| Msa0124960 | Msa0311900 | 0.814659 | 1.376641e-51 | -8.615850e-47 |
| Msa0145430 | Msa0311900 | 0.800389 | 1.474997e-48 | -8.615850e-47 |
| Msa0154730 | Msa0311900 | 0.816138 | 6.454020e-52 | -8.615850e-47 |
| Msa0191260 | Msa0311900 | 0.954006 | 7.791986e-112 | -8.615850e-47 |
| Msa0192890 | Msa0311900 | 0.802447 | 5.586555e-49 | -8.615850e-47 |
| Msa0311900 | Msa0316140 | 0.824518 | 7.729809e-54 | -8.615850e-47 |
| Msa0311900 | Msa0342590 | 0.806083 | 9.771714e-50 | -8.615850e-47 |
| Msa0311900 | Msa0511380 | 0.811615 | 6.407110e-51 | -8.615850e-47 |
| Msa0311900 | Msa0700410 | 0.811546 | 6.633905e-51 | -8.615850e-47 |
| Msa0311900 | Msa0829580 | 0.947470 | 6.318896e-106 | -8.615850e-47 |
| Msa0311900 | Msa1026970 | 0.844603 | 6.827333e-59 | -8.615850e-47 |
| Msa0311900 | Msa1111900 | 0.817834 | 2.684207e-52 | -8.615850e-47 |
| Msa0311900 | Msa1133760 | 0.844572 | 6.957678e-59 | -8.615850e-47 |
| Msa0311900 | Msa1154310 | 0.825298 | 5.059831e-54 | -8.615850e-47 |
| Msa0311900 | Msa1208530 | 0.800060 | 1.720868e-48 | -8.615850e-47 |
| Msa0311900 | Msa1369830 | 0.800733 | 1.255044e-48 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0311900 | MtrunA17_Chr2g0281091 | 94.667 | 75 | 4 | 0 | 1 | 75 | 15 | 89 | 2.96e-48 | 147 |
| Msa0311900 | MtrunA17_Chr2g0323571 | 42.105 | 76 | 38 | 3 | 3 | 75 | 19 | 91 | 1.00e-11 | 55.1 |
| Msa0311900 | MtrunA17_Chr2g0323671 | 42.105 | 76 | 38 | 3 | 3 | 75 | 19 | 91 | 1.99e-11 | 54.3 |
| Msa0311900 | MtrunA17_Chr2g0323631 | 42.105 | 76 | 38 | 3 | 3 | 75 | 19 | 91 | 2.95e-11 | 53.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 12 sgRNAs with CRISPR-Local
Find 9 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGGATTTCTTAGATCAATT+TGG | 0.263324 | 2_4:-6862692 | None:intergenic |
| AACTGCAATGAGAACGCTTC+AGG | 0.302945 | 2_4:+6862466 | None:intergenic |
| CTGTCATGTACTTGAGCTGC+TGG | 0.406277 | 2_4:+6862666 | Msa0311900:CDS |
| AACTTCTTCAAAGCAAGTTA+AGG | 0.411883 | 2_4:+6862544 | Msa0311900:CDS |
| CTTCAAAGCAAGTTAAGGCT+AGG | 0.439718 | 2_4:+6862549 | Msa0311900:CDS |
| TGTCATGTACTTGAGCTGCT+GGG | 0.470465 | 2_4:+6862667 | Msa0311900:CDS |
| AATTCATCTTCTACTTTCAA+AGG | 0.482157 | 2_4:+6862599 | Msa0311900:CDS |
| TTCAAAGCAAGTTAAGGCTA+GGG | 0.484226 | 2_4:+6862550 | Msa0311900:CDS |
| ACTGCAATGAGAACGCTTCA+GGG | 0.509342 | 2_4:+6862467 | None:intergenic |
| AACGTGCTGAAGAGTCTCTT+AGG | 0.527974 | 2_4:+6862642 | Msa0311900:CDS |
| GTCATGTACTTGAGCTGCTG+GGG | 0.565171 | 2_4:+6862668 | Msa0311900:CDS |
| CAAAGGTAGCAACAGTGACA+AGG | 0.606257 | 2_4:+6862616 | Msa0311900:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AATTCATCTTCTACTTTCAA+AGG | + | chr2_4:6862599-6862618 | Msa0311900:CDS | 25.0% |
| AACTTCTTCAAAGCAAGTTA+AGG | + | chr2_4:6862544-6862563 | Msa0311900:CDS | 30.0% | |
| TTCAAAGCAAGTTAAGGCTA+GGG | + | chr2_4:6862550-6862569 | Msa0311900:CDS | 35.0% | |
| CTTCAAAGCAAGTTAAGGCT+AGG | + | chr2_4:6862549-6862568 | Msa0311900:CDS | 40.0% | |
| CAAAGGTAGCAACAGTGACA+AGG | + | chr2_4:6862616-6862635 | Msa0311900:CDS | 45.0% | |
| AACGTGCTGAAGAGTCTCTT+AGG | + | chr2_4:6862642-6862661 | Msa0311900:CDS | 45.0% | |
| TGTCATGTACTTGAGCTGCT+GGG | + | chr2_4:6862667-6862686 | Msa0311900:CDS | 45.0% | |
| CTGTCATGTACTTGAGCTGC+TGG | + | chr2_4:6862666-6862685 | Msa0311900:CDS | 50.0% | |
| GTCATGTACTTGAGCTGCTG+GGG | + | chr2_4:6862668-6862687 | Msa0311900:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_4 | gene | 6862473 | 6862700 | 6862473 | ID=Msa0311900;Name=Msa0311900 |
| chr2_4 | mRNA | 6862473 | 6862700 | 6862473 | ID=Msa0311900-mRNA-1;Parent=Msa0311900;Name=Msa0311900-mRNA-1;_AED=0.09;_eAED=0.09;_QI=0|-1|0|1|-1|1|1|0|75 |
| chr2_4 | exon | 6862473 | 6862700 | 6862473 | ID=Msa0311900-mRNA-1:exon:2615;Parent=Msa0311900-mRNA-1 |
| chr2_4 | CDS | 6862473 | 6862700 | 6862473 | ID=Msa0311900-mRNA-1:cds;Parent=Msa0311900-mRNA-1 |
| Gene Sequence |
| Protein sequence |
