Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0829580 | AFK42939.1 | 95.506 | 89 | 4 | 0 | 1 | 89 | 1 | 89 | 1.10e-55 | 177 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0829580 | I3SRP7 | 95.506 | 89 | 4 | 0 | 1 | 89 | 1 | 89 | 5.25e-56 | 177 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0052780 | Msa0829580 | 0.804196 | 2.425704e-49 | -8.615850e-47 |
| Msa0080020 | Msa0829580 | 0.803578 | 3.260810e-49 | -8.615850e-47 |
| Msa0626920 | Msa0829580 | 0.813620 | 2.333957e-51 | -8.615850e-47 |
| Msa0651870 | Msa0829580 | 0.801503 | 8.730151e-49 | -8.615850e-47 |
| Msa0651890 | Msa0829580 | 0.803105 | 4.086240e-49 | -8.615850e-47 |
| Msa0700410 | Msa0829580 | 0.813067 | 3.087114e-51 | -8.615850e-47 |
| Msa0829580 | Msa1026970 | 0.827377 | 1.617861e-54 | -8.615850e-47 |
| Msa0829580 | Msa1111900 | 0.802413 | 5.677460e-49 | -8.615850e-47 |
| Msa0829580 | Msa1133760 | 0.828033 | 1.125323e-54 | -8.615850e-47 |
| Msa0829580 | Msa1154310 | 0.810209 | 1.291259e-50 | -8.615850e-47 |
| Msa0829580 | Msa1208530 | 0.801483 | 8.814391e-49 | -8.615850e-47 |
| Msa0829580 | Msa1369830 | 0.801874 | 7.329684e-49 | -8.615850e-47 |
| Msa0124960 | Msa0829580 | 0.800220 | 1.596181e-48 | -8.615850e-47 |
| Msa0145430 | Msa0829580 | 0.813830 | 2.098507e-51 | -8.615850e-47 |
| Msa0154730 | Msa0829580 | 0.803404 | 3.543622e-49 | -8.615850e-47 |
| Msa0191260 | Msa0829580 | 0.921612 | 2.862428e-88 | -8.615850e-47 |
| Msa0307380 | Msa0829580 | 0.805955 | 1.039663e-49 | -8.615850e-47 |
| Msa0311900 | Msa0829580 | 0.947470 | 6.318896e-106 | -8.615850e-47 |
| Msa0316140 | Msa0829580 | 0.811363 | 7.268360e-51 | -8.615850e-47 |
| Msa0511380 | Msa0829580 | 0.800669 | 1.292919e-48 | -8.615850e-47 |
| Msa0578460 | Msa0829580 | 0.803747 | 3.007625e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0829580 | MtrunA17_Chr2g0281091 | 95.506 | 89 | 4 | 0 | 1 | 89 | 1 | 89 | 1.01e-59 | 177 |
| Msa0829580 | MtrunA17_Chr2g0323571 | 42.105 | 76 | 38 | 3 | 17 | 89 | 19 | 91 | 1.97e-11 | 55.1 |
| Msa0829580 | MtrunA17_Chr2g0323671 | 42.105 | 76 | 38 | 3 | 17 | 89 | 19 | 91 | 3.94e-11 | 54.3 |
| Msa0829580 | MtrunA17_Chr2g0323631 | 42.105 | 76 | 38 | 3 | 17 | 89 | 19 | 91 | 4.69e-11 | 53.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 14 sgRNAs with CRISPR-Local
Find 12 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGGATTTCTTAGATCAATT+TGG | 0.263324 | 5_4:+2592379 | None:intergenic |
| AACTGCAATGAGAACGCTTC+AGG | 0.302945 | 5_4:-2592605 | Msa0829580:CDS |
| CTGTCATGTACTTGAGCTGC+TGG | 0.406277 | 5_4:-2592405 | Msa0829580:CDS |
| AACTTCTTCAAAGCAAGTTA+AGG | 0.411883 | 5_4:-2592527 | Msa0829580:CDS |
| CTTCAAAGCAAGTTAAGGCT+AGG | 0.439718 | 5_4:-2592522 | Msa0829580:CDS |
| GAAGCGTTCTCATTGCAGTT+TGG | 0.455396 | 5_4:+2592608 | None:intergenic |
| TGTCATGTACTTGAGCTGCT+GGG | 0.470465 | 5_4:-2592404 | Msa0829580:CDS |
| AATTCATCTTCTACTTTCAA+AGG | 0.482157 | 5_4:-2592472 | Msa0829580:CDS |
| TTCAAAGCAAGTTAAGGCTA+GGG | 0.484226 | 5_4:-2592521 | Msa0829580:CDS |
| ACTGCAATGAGAACGCTTCA+GGG | 0.509342 | 5_4:-2592604 | Msa0829580:CDS |
| AACGTGCTGAAGAGTCTCTT+AGG | 0.527974 | 5_4:-2592429 | Msa0829580:CDS |
| GGCATGAGTTTGAGGTACAT+AGG | 0.560477 | 5_4:-2592643 | Msa0829580:CDS |
| GTCATGTACTTGAGCTGCTG+GGG | 0.565171 | 5_4:-2592403 | Msa0829580:CDS |
| CAAAGGTAGCAACAGTGACA+AGG | 0.606257 | 5_4:-2592455 | Msa0829580:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AATTCATCTTCTACTTTCAA+AGG | - | chr5_4:2592561-2592580 | Msa0829580:CDS | 25.0% |
| AACTTCTTCAAAGCAAGTTA+AGG | - | chr5_4:2592506-2592525 | Msa0829580:CDS | 30.0% | |
| TTCAAAGCAAGTTAAGGCTA+GGG | - | chr5_4:2592512-2592531 | Msa0829580:CDS | 35.0% | |
| CTTCAAAGCAAGTTAAGGCT+AGG | - | chr5_4:2592511-2592530 | Msa0829580:CDS | 40.0% | |
| ! | GAAGCGTTCTCATTGCAGTT+TGG | + | chr5_4:2592428-2592447 | None:intergenic | 45.0% |
| AACTGCAATGAGAACGCTTC+AGG | - | chr5_4:2592428-2592447 | Msa0829580:CDS | 45.0% | |
| ACTGCAATGAGAACGCTTCA+GGG | - | chr5_4:2592429-2592448 | Msa0829580:CDS | 45.0% | |
| CAAAGGTAGCAACAGTGACA+AGG | - | chr5_4:2592578-2592597 | Msa0829580:CDS | 45.0% | |
| AACGTGCTGAAGAGTCTCTT+AGG | - | chr5_4:2592604-2592623 | Msa0829580:CDS | 45.0% | |
| TGTCATGTACTTGAGCTGCT+GGG | - | chr5_4:2592629-2592648 | Msa0829580:CDS | 45.0% | |
| CTGTCATGTACTTGAGCTGC+TGG | - | chr5_4:2592628-2592647 | Msa0829580:CDS | 50.0% | |
| GTCATGTACTTGAGCTGCTG+GGG | - | chr5_4:2592630-2592649 | Msa0829580:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_4 | gene | 2592393 | 2592662 | 2592393 | ID=Msa0829580;Name=Msa0829580 |
| chr5_4 | mRNA | 2592393 | 2592662 | 2592393 | ID=Msa0829580-mRNA-1;Parent=Msa0829580;Name=Msa0829580-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|-1|0|1|-1|1|1|0|89 |
| chr5_4 | exon | 2592393 | 2592662 | 2592393 | ID=Msa0829580-mRNA-1:exon:913;Parent=Msa0829580-mRNA-1 |
| chr5_4 | CDS | 2592393 | 2592662 | 2592393 | ID=Msa0829580-mRNA-1:cds;Parent=Msa0829580-mRNA-1 |
| Gene Sequence |
| Protein sequence |
