AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0210620

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0210620 MtrunA17_Chr2g0309251 97.203 429 9 2 1 429 1 426 0.0 861
Msa0210620 MtrunA17_Chr8g0380511 72.340 423 98 5 7 428 11 415 0.0 629
Msa0210620 MtrunA17_Chr7g0274951 54.374 423 187 4 12 428 17 439 1.24e-171 488
Msa0210620 MtrunA17_Chr1g0189961 52.874 435 186 7 9 429 22 451 7.61e-169 481
Msa0210620 MtrunA17_Chr3g0117271 53.808 407 164 7 25 429 112 496 4.95e-155 447
Msa0210620 MtrunA17_Chr8g0368161 54.124 388 155 3 43 429 83 448 4.03e-154 444
Msa0210620 MtrunA17_Chr4g0058771 54.847 392 154 7 40 429 111 481 2.07e-152 441
Msa0210620 MtrunA17_Chr5g0413131 53.351 388 152 5 46 429 101 463 3.67e-145 421
Msa0210620 MtrunA17_Chr2g0314451 48.205 390 174 8 41 429 43 405 6.51e-124 365
Msa0210620 MtrunA17_Chr2g0314461 48.082 391 169 7 43 429 46 406 6.59e-124 365
Msa0210620 MtrunA17_Chr8g0368241 46.173 392 180 5 43 429 49 414 6.18e-122 360
Msa0210620 MtrunA17_Chr2g0314471 47.423 388 176 7 43 429 46 406 9.97e-122 359
Msa0210620 MtrunA17_Chr3g0135381 41.944 391 211 4 45 426 51 434 8.38e-105 318
Msa0210620 MtrunA17_Chr8g0368201 48.148 324 139 6 123 429 9 320 3.26e-103 309
Msa0210620 MtrunA17_Chr6g0453411 41.534 378 201 8 62 429 8 375 1.31e-102 310
Msa0210620 MtrunA17_Chr3g0135371 39.695 393 221 6 45 429 51 435 4.92e-99 302
Msa0210620 MtrunA17_Chr8g0368291 39.394 429 212 12 12 429 14 405 4.62e-96 294
Msa0210620 MtrunA17_Chr2g0326371 49.835 303 130 8 132 429 75 360 6.22e-90 276
Msa0210620 MtrunA17_Chr8g0368341 40.650 369 180 8 63 429 4 335 6.79e-87 268
Msa0210620 MtrunA17_Chr3g0135401 39.521 334 188 4 105 426 16 347 2.00e-80 252
Msa0210620 MtrunA17_Chr8g0368181 58.031 193 80 1 237 429 2 193 7.95e-78 239
Msa0210620 MtrunA17_Chr1g0151871 38.961 308 167 4 123 429 40 327 1.18e-74 236
Msa0210620 MtrunA17_Chr8g0368221 46.094 256 110 6 50 304 30 258 6.57e-71 224
Msa0210620 MtrunA17_Chr8g0368361 51.282 195 92 3 237 429 2 195 5.49e-70 219
Msa0210620 MtrunA17_Chr3g0129891 50.971 206 93 3 225 428 180 379 7.57e-69 223
Msa0210620 MtrunA17_Chr3g0129891 44.615 130 53 4 43 165 79 196 2.57e-19 89.4
Msa0210620 MtrunA17_Chr8g0368331 41.877 277 129 4 182 429 13 286 8.44e-67 214
Msa0210620 MtrunA17_Chr8g0368281 46.632 193 102 1 237 428 2 194 1.25e-57 187
Msa0210620 MtrunA17_Chr3g0135431 33.611 360 152 9 36 321 35 381 6.84e-55 187
Msa0210620 MtrunA17_Chr3g0138811 32.353 374 206 15 59 425 53 386 3.42e-51 177
Msa0210620 MtrunA17_Chr3g0135441 36.626 243 131 5 190 429 10 232 4.16e-51 172
Msa0210620 MtrunA17_Chr8g0368351 41.379 232 102 8 11 238 47 248 4.64e-45 157
Msa0210620 MtrunA17_Chr8g0368261 57.480 127 52 2 304 429 4 129 5.06e-45 152
Msa0210620 MtrunA17_Chr2g0302691 30.376 372 211 13 60 423 37 368 3.61e-42 152
Msa0210620 MtrunA17_Chr8g0339871 38.211 246 137 12 192 428 2 241 2.06e-41 147
Msa0210620 MtrunA17_Chr8g0368231 53.175 126 57 2 305 429 8 132 5.36e-41 142
Msa0210620 MtrunA17_Chr8g0391201 36.066 244 141 11 193 428 3 239 1.88e-38 139
Msa0210620 MtrunA17_Chr8g0368381 47.788 113 59 0 237 349 2 114 7.07e-38 134
Msa0210620 MtrunA17_Chr5g0445181 32.130 277 150 11 90 366 61 299 3.99e-33 126
Msa0210620 MtrunA17_Chr3g0135451 44.697 132 65 2 35 158 28 159 1.28e-28 111
Msa0210620 MtrunA17_Chr8g0368171 32.068 237 116 5 5 238 10 204 4.01e-27 107
Msa0210620 MtrunA17_Chr8g0353931 30.660 212 113 7 33 238 20 203 1.54e-23 97.8
Msa0210620 MtrunA17_Chr2g0302651 30.942 223 122 11 215 428 1 200 3.59e-22 94.0
Msa0210620 MtrunA17_Chr8g0353941 35.638 188 111 7 242 425 1 182 1.02e-21 92.0
Msa0210620 MtrunA17_Chr5g0426971 28.390 236 142 7 190 423 46 256 5.11e-21 92.0
Msa0210620 MtrunA17_Chr5g0434771 35.849 159 94 5 250 406 12 164 1.39e-20 89.0
Msa0210620 MtrunA17_Chr8g0353971 32.164 171 108 4 257 423 4 170 1.26e-19 85.9
Msa0210620 MtrunA17_Chr8g0353961 35.036 137 65 4 31 156 12 135 1.39e-19 85.1
Msa0210620 MtrunA17_Chr8g0368321 41.176 119 52 4 41 158 41 142 1.10e-17 80.5
Msa0210620 MtrunA17_Chr8g0368271 33.548 155 86 6 9 158 11 153 1.54e-16 77.8
Msa0210620 MtrunA17_Chr5g0443801 36.697 109 67 2 192 300 21 127 2.19e-15 73.9
Msa0210620 MtrunA17_Chr3g0135411 61.224 49 19 0 45 93 64 112 6.45e-14 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0210620 AT3G13510.1 70.396 429 109 5 1 429 9 419 0.0 625
Msa0210620 AT1G55360.1 70.862 429 107 5 1 429 12 422 0.0 622
Msa0210620 AT5G56530.2 69.811 424 109 5 5 428 15 419 0.0 615
Msa0210620 AT5G56530.1 69.811 424 109 5 5 428 15 419 0.0 615
Msa0210620 AT2G44210.1 68.434 396 119 5 35 429 25 415 0.0 576
Msa0210620 AT2G44210.2 63.615 426 119 6 35 429 25 445 0.0 560
Msa0210620 AT5G18460.1 58.673 392 153 6 40 428 44 429 3.04e-164 469
Msa0210620 AT2G44220.1 51.928 389 170 4 42 429 31 403 8.07e-157 449
Msa0210620 AT1G10750.1 55.243 391 151 5 41 429 99 467 1.27e-154 446
Msa0210620 AT5G50150.1 53.964 391 157 5 41 429 51 420 8.73e-153 439
Msa0210620 AT1G23340.2 50.116 431 191 6 1 429 1 409 2.55e-151 435
Msa0210620 AT1G23340.1 50.116 431 191 6 1 429 1 409 2.55e-151 435
Msa0210620 AT1G70550.1 53.964 391 156 6 41 429 97 465 3.21e-149 432
Msa0210620 AT2G44240.1 47.897 428 192 7 3 429 5 402 1.86e-140 407
Msa0210620 AT2G17750.1 44.762 420 202 7 9 428 6 395 8.22e-133 388
Msa0210620 AT2G19360.1 46.189 433 199 7 14 428 9 425 1.33e-130 383
Msa0210620 AT2G44250.1 44.419 430 203 10 5 428 9 408 6.72e-121 358
Msa0210620 AT3G48230.1 43.669 387 187 11 50 429 11 373 1.05e-109 328
Msa0210620 AT5G25950.1 40.842 404 208 9 44 429 42 432 1.75e-93 288
Msa0210620 AT1G23340.3 48.366 306 134 6 1 304 1 284 3.90e-93 283
Msa0210620 AT5G19170.1 40.000 390 201 10 40 423 3 365 8.06e-93 285
Msa0210620 AT2G44250.2 45.833 312 159 7 121 428 20 325 2.59e-91 279
Msa0210620 AT2G20170.1 39.075 389 206 13 42 423 32 396 2.16e-84 264
Msa0210620 AT2G20170.2 39.075 389 206 13 42 423 32 396 2.16e-84 264
Msa0210620 AT5G25960.1 39.075 389 180 8 44 429 23 357 1.37e-81 255
Msa0210620 AT2G20170.3 35.990 389 201 13 42 423 32 379 4.89e-73 234
Msa0210620 AT4G23370.1 35.677 384 215 13 50 423 40 401 2.66e-70 228
Msa0210620 AT4G23355.1 35.696 395 210 14 42 423 35 398 1.28e-68 223
Msa0210620 AT4G23350.1 33.419 389 229 10 42 423 35 400 3.49e-67 219
Msa0210620 AT4G23390.1 35.770 383 211 14 50 423 41 397 2.65e-66 217
Msa0210620 AT4G23365.1 33.085 402 211 14 42 423 35 398 9.97e-66 216
Msa0210620 AT4G23360.1 34.097 393 220 12 42 423 35 399 4.85e-64 211
Msa0210620 AT4G23380.1 32.143 392 227 11 42 423 36 398 4.26e-62 206
Msa0210620 AT1G70550.2 49.770 217 85 6 41 255 97 291 1.81e-60 199
Msa0210620 AT2G35250.1 35.440 364 182 12 67 425 24 339 1.35e-54 185
Msa0210620 AT1G10750.2 42.797 236 105 7 46 276 105 315 9.83e-53 180
Msa0210620 AT1G10190.1 33.085 402 222 18 35 423 27 394 2.23e-49 172
Msa0210620 AT4G15050.1 33.333 405 217 19 35 423 27 394 2.29e-47 167
Msa0210620 AT4G15053.1 31.378 392 231 15 42 423 38 401 2.32e-46 165
Msa0210620 AT5G46810.1 30.000 370 212 13 70 423 11 349 2.67e-43 155
Msa0210620 AT4G17505.1 29.301 372 192 10 58 426 18 321 3.96e-41 149
Msa0210620 AT2G27320.1 33.016 315 161 10 69 380 30 297 6.61e-41 148
Msa0210620 AT5G46820.1 27.532 385 221 13 55 423 5 347 1.62e-40 148
Msa0210620 AT2G24950.1 29.206 428 248 19 8 423 25 409 1.38e-39 147
Msa0210620 AT5G05030.1 30.469 384 209 18 54 425 22 359 1.68e-39 145
Msa0210620 AT4G23373.1 32.700 263 130 9 13 274 7 223 7.32e-39 142
Msa0210620 AT4G10220.1 29.008 393 230 16 42 423 44 398 1.97e-38 143
Msa0210620 AT5G25410.1 26.862 376 229 12 54 423 28 363 2.36e-36 137
Msa0210620 AT5G60380.1 27.778 378 221 14 57 423 31 367 6.07e-35 133
Msa0210620 AT5G46200.1 27.110 391 232 13 70 423 31 405 8.64e-35 134
Msa0210620 AT4G10210.1 27.857 420 251 16 1 406 3 384 1.16e-32 127
Msa0210620 AT4G17860.1 28.378 370 202 16 70 423 29 351 4.29e-32 125
Msa0210620 AT4G23080.1 27.064 436 262 17 10 425 7 406 2.03e-30 121
Msa0210620 AT2G38255.1 33.333 225 105 7 70 292 14 195 2.51e-29 114
Msa0210620 AT5G11660.1 30.675 326 187 14 114 425 4 304 6.13e-29 115
Msa0210620 AT5G25415.1 27.219 338 205 13 105 425 19 332 2.94e-23 100
Msa0210620 AT2G03930.2 42.105 114 62 3 170 281 31 142 7.15e-23 95.1
Msa0210620 AT5G36680.1 27.213 305 181 11 131 423 52 327 9.31e-20 90.1
Msa0210620 AT5G36770.1 27.213 305 181 11 131 423 52 327 9.31e-20 90.1
Msa0210620 AT5G37520.1 32.328 232 116 13 199 424 2 198 6.43e-19 85.1
Msa0210620 AT5G04937.1 30.000 210 120 7 221 423 9 198 2.92e-18 83.2

Find 145 sgRNAs with CRISPR-Local

Find 273 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTAGCTCTGTTCAAAATTT+TGG 0.058994 2_1:-50841583 Msa0210620:CDS
ATCCACCAGTTTCCCTCTTT+TGG 0.121092 2_1:+50839772 None:intergenic
AATTCAGTCTGTCTCAAATT+TGG 0.126548 2_1:-50841007 Msa0210620:CDS
TTCCCTTCTGGATGGAAATT+TGG 0.152045 2_1:+50841748 None:intergenic
ATATGTATATTTATTTATTT+TGG 0.152989 2_1:-50838990 Msa0210620:three_prime_UTR
ACACTAGACTCTTCACTTAT+TGG 0.158217 2_1:-50840604 Msa0210620:CDS
CATCCAGAAGGGAAAACTTT+TGG 0.204617 2_1:-50841739 Msa0210620:CDS
GCTTTAATGGAGGCCAATTT+TGG 0.214424 2_1:-50842505 Msa0210620:exon
TCTGTCTCAAATTTGGATTT+TGG 0.224441 2_1:-50841000 Msa0210620:CDS
CCAGAACAAGTGACATATTA+AGG 0.229209 2_1:-50841606 Msa0210620:CDS
CATAGGATAAGCTTCAAAAT+TGG 0.235823 2_1:-50842770 Msa0210620:five_prime_UTR
TTCTGCCAAAGCTGAGTTAT+AGG 0.242996 2_1:+50841670 None:intergenic
TGTTATAATCTCCTTTGTTC+TGG 0.263241 2_1:-50840447 Msa0210620:CDS
TGCATTTGGAGAAGCTTTAA+TGG 0.266793 2_1:-50842518 Msa0210620:intron
CCTTAATATGTCACTTGTTC+TGG 0.271266 2_1:+50841606 None:intergenic
CTTGTTCTGGTCCTTCTTAT+AGG 0.284686 2_1:+50841619 None:intergenic
TTTGGAAATGACCATGTTCT+AGG 0.286569 2_1:-50839745 Msa0210620:CDS
CCAAGATGCTTATATCATAT+TGG 0.295235 2_1:+50840355 None:intergenic
CCTGAATCTTGTGATCTTTG+AGG 0.299817 2_1:+50841908 None:intergenic
GCAGAAGCATAATCCAAAAT+TGG 0.311137 2_1:+50842492 None:intergenic
GTGTAAGTACCAAGATCTTT+TGG 0.311380 2_1:+50839520 None:intergenic
TGGATTATGCTTCTGCTTGT+TGG 0.325487 2_1:-50842485 Msa0210620:CDS
TGGGGCACCTACTTCTATTA+TGG 0.331136 2_1:-50839451 Msa0210620:CDS
GAAAGCTGGTGGGTTGTTAA+TGG 0.338033 2_1:-50839047 Msa0210620:three_prime_UTR
GGAAACTGGTGGATGCAATT+TGG 0.341439 2_1:-50839763 Msa0210620:CDS
ATAAAAGATCAAGGACAGTT+AGG 0.341879 2_1:+50839412 None:intergenic
TGAGACAGACTGAATTCATT+AGG 0.345968 2_1:+50841014 None:intergenic
ATATCCCCACTTTCTAACTA+TGG 0.346949 2_1:-50840384 Msa0210620:CDS
AACTGGTAGTGCTGGTGATT+GGG 0.351854 2_1:-50839470 Msa0210620:CDS
AAAAGTTATCTCTACCTTGA+TGG 0.356984 2_1:+50842321 None:intergenic
CCTCAAAGATCACAAGATTC+AGG 0.363603 2_1:-50841908 Msa0210620:intron
ATCTTGTGATCTTTGAGGTC+AGG 0.364868 2_1:+50841913 None:intergenic
CAAGATGCTTATATCATATT+GGG 0.373261 2_1:+50840356 None:intergenic
GCTCTCACCACCACCGTTCC+CGG 0.378518 2_1:+50842411 None:intergenic
AACTGTTATAATGTCAAAAC+TGG 0.390356 2_1:-50839487 Msa0210620:CDS
TTATTGTCTCCATACAAATC+AGG 0.392038 2_1:+50840626 None:intergenic
GGAACAGAATATTCTCTAGT+AGG 0.392533 2_1:-50839025 Msa0210620:three_prime_UTR
TTGGTTCCTTTCACTTGTGC+TGG 0.399660 2_1:-50842457 Msa0210620:CDS
GCTTTCACAGCTCCAGAGAT+AGG 0.400467 2_1:+50839064 None:intergenic
CTACTTCTATTATGGTGGTC+CGG 0.402420 2_1:-50839443 Msa0210620:CDS
TAAGCTTCAAAATTGGGTTA+AGG 0.406474 2_1:-50842763 Msa0210620:five_prime_UTR
GTGGAGGGAGGTGAATTCTA+TGG 0.407436 2_1:-50841080 Msa0210620:CDS
ATAGGATAAGCTTCAAAATT+GGG 0.416322 2_1:-50842769 Msa0210620:five_prime_UTR
CAAAGGCGACCATAAACGTT+TGG 0.418700 2_1:-50841055 Msa0210620:CDS
TTGATGGTGACAACAAGTTA+AGG 0.430415 2_1:-50839546 Msa0210620:CDS
GCCACCATCAGCACCACCAC+CGG 0.431095 2_1:-50842430 Msa0210620:CDS
TAGGTCAGCCCTGATTTGTA+TGG 0.432543 2_1:-50840635 Msa0210620:CDS
TCTACCTTGATGGACTTAAC+AGG 0.434566 2_1:+50842331 None:intergenic
ATGACCATGTTCTAGGATAC+TGG 0.437458 2_1:-50839738 Msa0210620:CDS
CAAAGCCTATAACTCAGCTT+TGG 0.444736 2_1:-50841675 Msa0210620:CDS
ACCACCACCGTTCCCGGTGG+TGG 0.447471 2_1:+50842417 None:intergenic
TAAAAGATCAAGGACAGTTA+GGG 0.448907 2_1:+50839413 None:intergenic
ACAGCACACATCAACACAAA+TGG 0.449226 2_1:-50839632 Msa0210620:CDS
CCAATATGATATAAGCATCT+TGG 0.452731 2_1:-50840355 Msa0210620:CDS
TTAAGGGCTCCAAAAGATCT+TGG 0.459260 2_1:-50839529 Msa0210620:CDS
AAACTGGTAGTGCTGGTGAT+TGG 0.461992 2_1:-50839471 Msa0210620:CDS
AGGACCCAAAAGAGGGAAAC+TGG 0.463817 2_1:-50839777 Msa0210620:CDS
TCAGTTAGGTATGAGAATAA+AGG 0.467064 2_1:+50839709 None:intergenic
CTTGACTCAGAGTGGTCATC+AGG 0.468890 2_1:-50841503 Msa0210620:intron
TGATGGTGACAACAAGTTAA+GGG 0.475300 2_1:-50839545 Msa0210620:CDS
TGTTGGAGAAAGAGGGTGAT+GGG 0.475386 2_1:-50839164 Msa0210620:three_prime_UTR
ATCTCTGGAGCTGTGAAAGC+TGG 0.479478 2_1:-50839061 Msa0210620:three_prime_UTR
AAAGGCGACCATAAACGTTT+GGG 0.483661 2_1:-50841054 Msa0210620:CDS
TCATTGAGGCACTCTCAGTT+AGG 0.484694 2_1:+50839695 None:intergenic
TACTTCTATTATGGTGGTCC+GGG 0.485729 2_1:-50839442 Msa0210620:CDS
ATTAACAATGGTATAGCCCT+TGG 0.488395 2_1:-50840414 Msa0210620:CDS
GCCTTGCCAAAACCTTCATC+AGG 0.488665 2_1:+50839595 None:intergenic
ATGATATAAGCATCTTGGTC+TGG 0.490781 2_1:-50840350 Msa0210620:CDS
AGAGTGCCTCAATGATTGAA+TGG 0.494264 2_1:-50839687 Msa0210620:CDS
TGGATTGCAATTTAAAACTA+CGG 0.494301 2_1:-50839330 Msa0210620:intron
AGTGATGCATATCAAGCAAC+TGG 0.496993 2_1:-50840471 Msa0210620:CDS
AAGGAACAATTCCTATAAGA+AGG 0.498065 2_1:-50841630 Msa0210620:CDS
ACCACCACCGGGAACGGTGG+TGG 0.505459 2_1:-50842418 Msa0210620:CDS
CAGCATTGAAGCAGGCTGGC+AGG 0.505596 2_1:-50840952 Msa0210620:intron
GAACAGAATATTCTCTAGTA+GGG 0.506018 2_1:-50839024 Msa0210620:three_prime_UTR
AAAGCTGGTGGGTTGTTAAT+GGG 0.507478 2_1:-50839046 Msa0210620:three_prime_UTR
TTGGGTTAAGGTTAAGCCTA+AGG 0.507890 2_1:-50842751 Msa0210620:intron
CCACTCTGAGTCAAGATGTC+AGG 0.518935 2_1:+50841511 None:intergenic
CAGCACACATCAACACAAAT+GGG 0.520852 2_1:-50839631 Msa0210620:CDS
CAGAACAAGTGACATATTAA+GGG 0.521105 2_1:-50841605 Msa0210620:CDS
GAGTGCCTCAATGATTGAAT+GGG 0.527263 2_1:-50839686 Msa0210620:CDS
GTTCCCGGTGGTGGTGCTGA+TGG 0.531155 2_1:+50842426 None:intergenic
TTAGCATGCCATAGCTTATG+TGG 0.531230 2_1:-50841099 Msa0210620:intron
AATGTCAAAACTGGTAGTGC+TGG 0.534590 2_1:-50839478 Msa0210620:CDS
GGCACCTACTTCTATTATGG+TGG 0.539245 2_1:-50839448 Msa0210620:CDS
GATCTCAACAGCATTGAAGC+AGG 0.539985 2_1:-50840960 Msa0210620:CDS
GAAGAACCATAGTTAGAAAG+TGG 0.541399 2_1:+50840378 None:intergenic
TTGTGAAAGCAGAGTCCTGA+TGG 0.541959 2_1:-50841985 Msa0210620:intron
CCACCATCAGCACCACCACC+GGG 0.545767 2_1:-50842429 Msa0210620:CDS
AGGTCAGGGTGATCAAAAGC+TGG 0.547634 2_1:+50841928 None:intergenic
TCTGGAGCTGTGAAAGCTGG+TGG 0.550324 2_1:-50839058 Msa0210620:three_prime_UTR
ACCTCCCTCCACATAAGCTA+TGG 0.557000 2_1:+50841091 None:intergenic
CCCGGTGGTGGTGCTGATGG+TGG 0.557572 2_1:+50842429 None:intergenic
TGTAAGTTGAGCTACCACTT+AGG 0.562344 2_1:-50839237 Msa0210620:three_prime_UTR
TAATGTGTAGGACCCAAAAG+AGG 0.564000 2_1:-50839785 Msa0210620:intron
GGCTTTATTCAAATTAACAA+TGG 0.564459 2_1:-50840426 Msa0210620:CDS
TGGGGATATACTTGCTCCAA+GGG 0.564591 2_1:+50840398 None:intergenic
ACATCAACACAAATGGGAAG+TGG 0.565243 2_1:-50839625 Msa0210620:CDS
CAGTCAATAATATCTCCATC+AGG 0.565977 2_1:+50841970 None:intergenic
AGCTTTAACATAAAAGATCA+AGG 0.566363 2_1:+50839403 None:intergenic
GGGAACGGTGGTGGTGAGAG+CGG 0.566812 2_1:-50842409 Msa0210620:CDS
TCTTGTGATCTTTGAGGTCA+GGG 0.570285 2_1:+50841914 None:intergenic
AGGTATGAGAATAAAGGAGC+TGG 0.573128 2_1:+50839715 None:intergenic
GAAGCTCAAAATTCTCATTG+AGG 0.576114 2_1:-50839198 Msa0210620:three_prime_UTR
CTGGAGCTGTGAAAGCTGGT+GGG 0.576766 2_1:-50839057 Msa0210620:three_prime_UTR
ACCACCCCATTCAATCATTG+AGG 0.578345 2_1:+50839681 None:intergenic
AACGGTGGTGGTGAGAGCGG+TGG 0.580017 2_1:-50842406 Msa0210620:CDS
TATTGTCTCCATACAAATCA+GGG 0.581583 2_1:+50840627 None:intergenic
GTGGGGATATACTTGCTCCA+AGG 0.581673 2_1:+50840397 None:intergenic
TATACACCACATTACCTAAG+TGG 0.581964 2_1:+50839223 None:intergenic
TCAACAGCATTGAAGCAGGC+TGG 0.583391 2_1:-50840956 Msa0210620:CDS
AGGCCAAATTTCCATCCAGA+AGG 0.584529 2_1:-50841751 Msa0210620:CDS
CGGACCACCATAATAGAAGT+AGG 0.584855 2_1:+50839444 None:intergenic
TGGTTCAATTCAGAAACTTG+AGG 0.588867 2_1:-50842386 Msa0210620:CDS
TATAAGCATCTTGGTCTGGA+AGG 0.589005 2_1:-50840346 Msa0210620:intron
TTGTTGGAGAAAGAGGGTGA+TGG 0.593904 2_1:-50839165 Msa0210620:three_prime_UTR
AAGAACCATAGTTAGAAAGT+GGG 0.595857 2_1:+50840379 None:intergenic
ACTGGTAGTGCTGGTGATTG+GGG 0.595966 2_1:-50839469 Msa0210620:CDS
TTTGAATAAAGCCAGAACAA+AGG 0.597232 2_1:+50840436 None:intergenic
TGAGTCAAGATGTCAGGTAG+AGG 0.600193 2_1:+50841517 None:intergenic
ATCAGCACCACCACCGGGAA+CGG 0.601551 2_1:-50842424 Msa0210620:CDS
GCTCTGCCAGCACAAGTGAA+AGG 0.602569 2_1:+50842451 None:intergenic
CAGGGTGATCAAAAGCTGGT+TGG 0.603523 2_1:+50841932 None:intergenic
CTGGCCAGTATCCTAGAACA+TGG 0.608996 2_1:+50839734 None:intergenic
AGCACCACCACCGGGAACGG+TGG 0.613079 2_1:-50842421 Msa0210620:CDS
ACCGCCTGTTAAGTCCATCA+AGG 0.617331 2_1:-50842335 Msa0210620:intron
AGGTGAATTCTATGGAGCAA+AGG 0.618255 2_1:-50841072 Msa0210620:CDS
AATGTGTAGGACCCAAAAGA+GGG 0.621507 2_1:-50839784 Msa0210620:intron
GAGCTACCACTTAGGTAATG+TGG 0.640847 2_1:-50839229 Msa0210620:three_prime_UTR
AACAGAATATTCTCTAGTAG+GGG 0.643070 2_1:-50839023 Msa0210620:three_prime_UTR
GGCCAAATTTCCATCCAGAA+GGG 0.643729 2_1:-50841750 Msa0210620:CDS
CATGCCATAGCTTATGTGGA+GGG 0.644068 2_1:-50841095 Msa0210620:CDS
GCATGCCATAGCTTATGTGG+AGG 0.646750 2_1:-50841096 Msa0210620:CDS
AGAACCATAGTTAGAAAGTG+GGG 0.647282 2_1:+50840380 None:intergenic
ATTTGGAGAAGCTTTAATGG+AGG 0.650311 2_1:-50842515 Msa0210620:intron
GCTGTTGAGATCTTGACCAA+AGG 0.656530 2_1:+50840971 None:intergenic
GCCATAGCTTATGTGGAGGG+AGG 0.658878 2_1:-50841092 Msa0210620:CDS
GGACAGTTAGGGTTTCGACC+CGG 0.661025 2_1:+50839424 None:intergenic
GTAGTGAACTCTGAGTCTGA+TGG 0.662522 2_1:-50839655 Msa0210620:CDS
GCCTCAATGATTGAATGGGG+TGG 0.671790 2_1:-50839682 Msa0210620:CDS
ACCTTGATGGACTTAACAGG+CGG 0.693538 2_1:+50842334 None:intergenic
CCTGACATCTTGACTCAGAG+TGG 0.701149 2_1:-50841511 Msa0210620:CDS
ACCCAAAAGAGGGAAACTGG+TGG 0.707549 2_1:-50839774 Msa0210620:CDS
CTCACCACCACCGTTCCCGG+TGG 0.716489 2_1:+50842414 None:intergenic
AGTGCCTCAATGATTGAATG+GGG 0.753510 2_1:-50839685 Msa0210620:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAAAATATAGAACTTTTTA+TGG - chr2_1:50839115-50839134 Msa0210620:three_prime_UTR 10.0%
!!! TTGTAAAAAATTAAACTTTT+TGG - chr2_1:50839514-50839533 Msa0210620:CDS 10.0%
!!! TGTAAAAAATTAAACTTTTT+GGG - chr2_1:50839515-50839534 Msa0210620:CDS 10.0%
!!! ATTTTAGTATGTTTTTATAT+TGG - chr2_1:50839642-50839661 Msa0210620:CDS 10.0%
!!! GATTTAATTTTTAGATTTTT+AGG - chr2_1:50839693-50839712 Msa0210620:CDS 10.0%
!!! CAAAAAATAACATTTTAAAA+AGG + chr2_1:50841435-50841454 None:intergenic 10.0%
!!! AAAATGTTATTTTTTGTTAT+TGG - chr2_1:50841439-50841458 Msa0210620:intron 10.0%
!! TATAGCATATACAATTAAAT+GGG + chr2_1:50839090-50839109 None:intergenic 15.0%
!! TTATAGCATATACAATTAAA+TGG + chr2_1:50839091-50839110 None:intergenic 15.0%
!! TTTCTAAATTCAAACAATTA+TGG - chr2_1:50839959-50839978 Msa0210620:intron 15.0%
!! TTCTAAATTCAAACAATTAT+GGG - chr2_1:50839960-50839979 Msa0210620:intron 15.0%
!! AATTGGTTATTGAAAATTTA+TGG + chr2_1:50840447-50840466 None:intergenic 15.0%
!! AACATATACTATCATAAAAA+AGG + chr2_1:50840539-50840558 None:intergenic 15.0%
!! TAAAACAAATACATTTAATC+AGG + chr2_1:50840601-50840620 None:intergenic 15.0%
!! ATTGATACATATTAAATTCA+TGG + chr2_1:50841912-50841931 None:intergenic 15.0%
!! TGAATAAAAAAATAATGTGT+AGG - chr2_1:50841952-50841971 Msa0210620:CDS 15.0%
!!! TAGTTATTTCAAAAATATTC+AGG - chr2_1:50842177-50842196 Msa0210620:intron 15.0%
!!! TAGAACTTTTTATGGTTTTT+TGG - chr2_1:50839123-50839142 Msa0210620:three_prime_UTR 20.0%
!!! ATAACGGTTTTATTTCTAAT+TGG - chr2_1:50839488-50839507 Msa0210620:CDS 20.0%
!!! TAACGGTTTTATTTCTAATT+GGG - chr2_1:50839489-50839508 Msa0210620:CDS 20.0%
!! AAATGAACTATGTTTATGAA+TGG + chr2_1:50839720-50839739 None:intergenic 20.0%
!!! TAAACAAGTTGAAAAAGAAA+AGG + chr2_1:50839877-50839896 None:intergenic 20.0%
!! TCAATAATTGACGAATAAAT+TGG + chr2_1:50840464-50840483 None:intergenic 20.0%
!!! TCCTTTTGATTTTATTTTCT+TGG - chr2_1:50840622-50840641 Msa0210620:CDS 20.0%
!! TCCAAGAAAATAAAATCAAA+AGG + chr2_1:50840626-50840645 None:intergenic 20.0%
!! TTTAAAAGCTAATTTGACAT+CGG - chr2_1:50841747-50841766 Msa0210620:CDS 20.0%
! ATAGGATAAGCTTCAAAATT+GGG - chr2_1:50838980-50838999 Msa0210620:three_prime_UTR 25.0%
! TGTATGTAATATTCTACCTT+AGG + chr2_1:50839017-50839036 None:intergenic 25.0%
! CAAAGACTAATGAAGTTAAA+GGG + chr2_1:50839152-50839171 None:intergenic 25.0%
! ACAAAGACTAATGAAGTTAA+AGG + chr2_1:50839153-50839172 None:intergenic 25.0%
! CTTTATGAGAATTGTCAAAT+GGG + chr2_1:50839194-50839213 None:intergenic 25.0%
! ACTTTATGAGAATTGTCAAA+TGG + chr2_1:50839195-50839214 None:intergenic 25.0%
!!! TTAAACTTTTTGGGTTTGAA+AGG - chr2_1:50839524-50839543 Msa0210620:CDS 25.0%
! ATTGTAGTGAAAAAGACTAA+AGG + chr2_1:50839578-50839597 None:intergenic 25.0%
!!! AGTCTTTTTCACTACAATAA+TGG - chr2_1:50839580-50839599 Msa0210620:CDS 25.0%
!!! TCTTTCTTTTATGTGTCATT+TGG - chr2_1:50839923-50839942 Msa0210620:intron 25.0%
! AAAAATGGTATTTGTTCTGA+AGG - chr2_1:50840100-50840119 Msa0210620:intron 25.0%
! GTTATTGAAAATTTATGGAC+CGG + chr2_1:50840442-50840461 None:intergenic 25.0%
! TAGTATATGTTACTATAGCA+TGG - chr2_1:50840548-50840567 Msa0210620:intron 25.0%
! TTTAATGAAAGAACAGAAAG+TGG + chr2_1:50841197-50841216 None:intergenic 25.0%
!!! GGCTTTATTCAAATTAACAA+TGG - chr2_1:50841323-50841342 Msa0210620:intron 25.0%
! CAAGATGCTTATATCATATT+GGG + chr2_1:50841396-50841415 None:intergenic 25.0%
!! GGCATATTTTCTTTCTATAA+TGG + chr2_1:50841465-50841484 None:intergenic 25.0%
! AAATAACAGCTTAGAATCAT+TGG + chr2_1:50841790-50841809 None:intergenic 25.0%
! AAATTTGAATTCGTACACAT+GGG - chr2_1:50841839-50841858 Msa0210620:intron 25.0%
!!! AAAAATATTCAGGTTGTTGA+TGG - chr2_1:50842187-50842206 Msa0210620:intron 25.0%
! AACTGTTATAATGTCAAAAC+TGG - chr2_1:50842262-50842281 Msa0210620:intron 25.0%
!! AGCTTTAACATAAAAGATCA+AGG + chr2_1:50842349-50842368 None:intergenic 25.0%
!! TTTTAAATTGCAGGTAAATG+CGG - chr2_1:50842373-50842392 Msa0210620:CDS 25.0%
!! TGGATTGCAATTTAAAACTA+CGG - chr2_1:50842419-50842438 Msa0210620:CDS 25.0%
!! AGCTTTTCATTAATCAAACA+TGG + chr2_1:50842787-50842806 None:intergenic 25.0%
!!! GTTGTTGTTGTTTGTTTTGT+TGG - chr2_1:50838949-50838968 Msa0210620:three_prime_UTR 30.0%
!!! TTGTTGTTTGTTTTGTTGGT+GGG - chr2_1:50838953-50838972 Msa0210620:three_prime_UTR 30.0%
CATAGGATAAGCTTCAAAAT+TGG - chr2_1:50838979-50838998 Msa0210620:three_prime_UTR 30.0%
TAAGCTTCAAAATTGGGTTA+AGG - chr2_1:50838986-50839005 Msa0210620:three_prime_UTR 30.0%
AAAAGTTATCTCTACCTTGA+TGG + chr2_1:50839431-50839450 None:intergenic 30.0%
!! CTTTTTCATTGGTTGCATAA+CGG - chr2_1:50839472-50839491 Msa0210620:CDS 30.0%
!! TTGAAAGGAACTTTAGTTCT+TGG - chr2_1:50839539-50839558 Msa0210620:CDS 30.0%
!! TGAAAGGAACTTTAGTTCTT+GGG - chr2_1:50839540-50839559 Msa0210620:CDS 30.0%
TATGCAAGTGTACTTTGTTA+TGG - chr2_1:50839739-50839758 Msa0210620:CDS 30.0%
AAGGAACAATTCCTATAAGA+AGG - chr2_1:50840119-50840138 Msa0210620:intron 30.0%
CAGAACAAGTGACATATTAA+GGG - chr2_1:50840144-50840163 Msa0210620:intron 30.0%
TAGGTGCATTATAAGTAACA+TGG + chr2_1:50840283-50840302 None:intergenic 30.0%
!! CATTAGGTTGCTGAATTTTT+GGG + chr2_1:50840722-50840741 None:intergenic 30.0%
!! TCATTAGGTTGCTGAATTTT+TGG + chr2_1:50840723-50840742 None:intergenic 30.0%
AATTCAGTCTGTCTCAAATT+TGG - chr2_1:50840742-50840761 Msa0210620:intron 30.0%
! TCTGTCTCAAATTTGGATTT+TGG - chr2_1:50840749-50840768 Msa0210620:intron 30.0%
TGTTTGACTCAATTGGTTAA+TGG - chr2_1:50840852-50840871 Msa0210620:intron 30.0%
TATTGTCTCCATACAAATCA+GGG + chr2_1:50841125-50841144 None:intergenic 30.0%
TTATTGTCTCCATACAAATC+AGG + chr2_1:50841126-50841145 None:intergenic 30.0%
TGTTATAATCTCCTTTGTTC+TGG - chr2_1:50841302-50841321 Msa0210620:intron 30.0%
TTTGAATAAAGCCAGAACAA+AGG + chr2_1:50841316-50841335 None:intergenic 30.0%
AAGAACCATAGTTAGAAAGT+GGG + chr2_1:50841373-50841392 None:intergenic 30.0%
CCAAGATGCTTATATCATAT+TGG + chr2_1:50841397-50841416 None:intergenic 30.0%
CCAATATGATATAAGCATCT+TGG - chr2_1:50841394-50841413 Msa0210620:intron 30.0%
TTGTAGTGTTAGTTAAGTGT+GGG + chr2_1:50841487-50841506 None:intergenic 30.0%
ATTGTAGTGTTAGTTAAGTG+TGG + chr2_1:50841488-50841507 None:intergenic 30.0%
ATGCGTGTATAATGTATGTA+TGG - chr2_1:50841602-50841621 Msa0210620:CDS 30.0%
! AGTATTGGATGAAAATCAGA+CGG - chr2_1:50841715-50841734 Msa0210620:CDS 30.0%
TAAATGCATGGATTCTATGA+CGG - chr2_1:50841812-50841831 Msa0210620:intron 30.0%
GAAATTTGAATTCGTACACA+TGG - chr2_1:50841838-50841857 Msa0210620:intron 30.0%
TCAGTTAGGTATGAGAATAA+AGG + chr2_1:50842043-50842062 None:intergenic 30.0%
TAAAAGATCAAGGACAGTTA+GGG + chr2_1:50842339-50842358 None:intergenic 30.0%
ATAAAAGATCAAGGACAGTT+AGG + chr2_1:50842340-50842359 None:intergenic 30.0%
! GATCTTTTATGTTAAAGCTG+TGG - chr2_1:50842350-50842369 Msa0210620:CDS 30.0%
CAAAAATTGTTGGAGAAAGA+GGG - chr2_1:50842578-50842597 Msa0210620:intron 30.0%
GAACAGAATATTCTCTAGTA+GGG - chr2_1:50842725-50842744 Msa0210620:intron 30.0%
AACAGAATATTCTCTAGTAG+GGG - chr2_1:50842726-50842745 Msa0210620:intron 30.0%
!!! GTTGTTGTTTGTTTTGTTGG+TGG - chr2_1:50838952-50838971 Msa0210620:three_prime_UTR 35.0%
!!! TGTTGTTTGTTTTGTTGGTG+GGG - chr2_1:50838954-50838973 Msa0210620:three_prime_UTR 35.0%
! CATGCTTTTGCATTTGCATT+TGG - chr2_1:50839217-50839236 Msa0210620:three_prime_UTR 35.0%
! TGCATTTGGAGAAGCTTTAA+TGG - chr2_1:50839231-50839250 Msa0210620:three_prime_UTR 35.0%
! ATTTGGAGAAGCTTTAATGG+AGG - chr2_1:50839234-50839253 Msa0210620:three_prime_UTR 35.0%
! GCAGAAGCATAATCCAAAAT+TGG + chr2_1:50839260-50839279 None:intergenic 35.0%
TGGTTCAATTCAGAAACTTG+AGG - chr2_1:50839363-50839382 Msa0210620:intron 35.0%
GAAAAAGTGACAGAGTGAAA+AGG + chr2_1:50839459-50839478 None:intergenic 35.0%
!!! TCACTCTGTCACTTTTTCAT+TGG - chr2_1:50839461-50839480 Msa0210620:CDS 35.0%
!! AACTTTAGTTCTTGGGAGTT+GGG - chr2_1:50839547-50839566 Msa0210620:CDS 35.0%
CAGTCAATAATATCTCCATC+AGG + chr2_1:50839782-50839801 None:intergenic 35.0%
! CCTTAATATGTCACTTGTTC+TGG + chr2_1:50840146-50840165 None:intergenic 35.0%
CCAGAACAAGTGACATATTA+AGG - chr2_1:50840143-50840162 Msa0210620:intron 35.0%
!!! GCTAGCTCTGTTCAAAATTT+TGG - chr2_1:50840166-50840185 Msa0210620:intron 35.0%
!! TAGTGACTTAAACTTGCTGT+AGG + chr2_1:50840302-50840321 None:intergenic 35.0%
!! TCATCCATGATTTGTTATGC+CGG - chr2_1:50840420-50840439 Msa0210620:CDS 35.0%
TGAGACAGACTGAATTCATT+AGG + chr2_1:50840738-50840757 None:intergenic 35.0%
!! TCTCAAATTTGGATTTTGGC+TGG - chr2_1:50840753-50840772 Msa0210620:intron 35.0%
CATCTTCTGTTTGACTCAAT+TGG - chr2_1:50840845-50840864 Msa0210620:intron 35.0%
! TTTTGGCCATGGTTGAAATT+CGG + chr2_1:50840910-50840929 None:intergenic 35.0%
! AGTGTGGTCAATGATTGTTT+TGG + chr2_1:50840927-50840946 None:intergenic 35.0%
ATTGGGAGATAAGAAAAGTG+TGG + chr2_1:50840943-50840962 None:intergenic 35.0%
! AAGGGAAATGGACTTCAATT+GGG + chr2_1:50840960-50840979 None:intergenic 35.0%
TTTCTGCTCTTGCAACAATT+AGG - chr2_1:50841095-50841114 Msa0210620:CDS 35.0%
ACACTAGACTCTTCACTTAT+TGG - chr2_1:50841145-50841164 Msa0210620:intron 35.0%
! AAAGTGGAGTGATTTGCATT+GGG + chr2_1:50841181-50841200 None:intergenic 35.0%
ATTAACAATGGTATAGCCCT+TGG - chr2_1:50841335-50841354 Msa0210620:intron 35.0%
ATATCCCCACTTTCTAACTA+TGG - chr2_1:50841365-50841384 Msa0210620:intron 35.0%
AGAACCATAGTTAGAAAGTG+GGG + chr2_1:50841372-50841391 None:intergenic 35.0%
GAAGAACCATAGTTAGAAAG+TGG + chr2_1:50841374-50841393 None:intergenic 35.0%
ATGATATAAGCATCTTGGTC+TGG - chr2_1:50841399-50841418 Msa0210620:intron 35.0%
TGTAGTGTTAGTTAAGTGTG+GGG + chr2_1:50841486-50841505 None:intergenic 35.0%
! AGTCAACAGCTTAGAAGTAT+TGG - chr2_1:50841700-50841719 Msa0210620:CDS 35.0%
!! GCTGTTATTTCGTAAATGCA+TGG - chr2_1:50841800-50841819 Msa0210620:intron 35.0%
TTTGGAAATGACCATGTTCT+AGG - chr2_1:50842004-50842023 Msa0210620:intron 35.0%
! CATTTTCCTGATGAAGGTTT+TGG - chr2_1:50842148-50842167 Msa0210620:intron 35.0%
!! TTGATGGTGACAACAAGTTA+AGG - chr2_1:50842203-50842222 Msa0210620:intron 35.0%
! TGATGGTGACAACAAGTTAA+GGG - chr2_1:50842204-50842223 Msa0210620:intron 35.0%
! GTGTAAGTACCAAGATCTTT+TGG + chr2_1:50842232-50842251 None:intergenic 35.0%
!!! AAGCTGTGGTTTTAAATTGC+AGG - chr2_1:50842364-50842383 Msa0210620:CDS 35.0%
TAAATTGCAATCCACAACTG+CGG + chr2_1:50842413-50842432 None:intergenic 35.0%
TATACACCACATTACCTAAG+TGG + chr2_1:50842529-50842548 None:intergenic 35.0%
GAAGCTCAAAATTCTCATTG+AGG - chr2_1:50842551-50842570 Msa0210620:intron 35.0%
TTGAGGTCTCCAAAAATTGT+TGG - chr2_1:50842568-50842587 Msa0210620:intron 35.0%
! CCTCTTTCTCCAACAATTTT+TGG + chr2_1:50842580-50842599 None:intergenic 35.0%
CCAAAAATTGTTGGAGAAAG+AGG - chr2_1:50842577-50842596 Msa0210620:intron 35.0%
GGAACAGAATATTCTCTAGT+AGG - chr2_1:50842724-50842743 Msa0210620:intron 35.0%
TTGGGTTAAGGTTAAGCCTA+AGG - chr2_1:50838998-50839017 Msa0210620:three_prime_UTR 40.0%
! GCTTTAATGGAGGCCAATTT+TGG - chr2_1:50839244-50839263 Msa0210620:three_prime_UTR 40.0%
TGGATTATGCTTCTGCTTGT+TGG - chr2_1:50839264-50839283 Msa0210620:three_prime_UTR 40.0%
!!! CTTCTGCTTGTTGGTTTTGT+TGG - chr2_1:50839273-50839292 Msa0210620:three_prime_UTR 40.0%
!! TCTACCTTGATGGACTTAAC+AGG + chr2_1:50839421-50839440 None:intergenic 40.0%
!! GAACTTTAGTTCTTGGGAGT+TGG - chr2_1:50839546-50839565 Msa0210620:CDS 40.0%
!! TCTTGTGATCTTTGAGGTCA+GGG + chr2_1:50839838-50839857 None:intergenic 40.0%
!! ATCTTGTGATCTTTGAGGTC+AGG + chr2_1:50839839-50839858 None:intergenic 40.0%
CCTGAATCTTGTGATCTTTG+AGG + chr2_1:50839844-50839863 None:intergenic 40.0%
CCTCAAAGATCACAAGATTC+AGG - chr2_1:50839841-50839860 Msa0210620:intron 40.0%
TTCCCTTCTGGATGGAAATT+TGG + chr2_1:50840004-50840023 None:intergenic 40.0%
! CAAAAGTTTTCCCTTCTGGA+TGG + chr2_1:50840012-50840031 None:intergenic 40.0%
! CATCCAGAAGGGAAAACTTT+TGG - chr2_1:50840010-50840029 Msa0210620:intron 40.0%
! TCTCCAAAAGTTTTCCCTTC+TGG + chr2_1:50840016-50840035 None:intergenic 40.0%
CAAAGCCTATAACTCAGCTT+TGG - chr2_1:50840074-50840093 Msa0210620:intron 40.0%
TTCTGCCAAAGCTGAGTTAT+AGG + chr2_1:50840082-50840101 None:intergenic 40.0%
! ACTCAGCTTTGGCAGAAAAA+TGG - chr2_1:50840085-50840104 Msa0210620:intron 40.0%
CTTGTTCTGGTCCTTCTTAT+AGG + chr2_1:50840133-50840152 None:intergenic 40.0%
ACTATAGCATGGAGATGCTT+TGG - chr2_1:50840559-50840578 Msa0210620:intron 40.0%
TTAGCATGCCATAGCTTATG+TGG - chr2_1:50840650-50840669 Msa0210620:intron 40.0%
AGGTGAATTCTATGGAGCAA+AGG - chr2_1:50840677-50840696 Msa0210620:intron 40.0%
AAAGGCGACCATAAACGTTT+GGG - chr2_1:50840695-50840714 Msa0210620:intron 40.0%
!! TTTTGGGTCCCAAACGTTTA+TGG + chr2_1:50840706-50840725 None:intergenic 40.0%
AGTCAAACAGAAGATGACAG+TGG + chr2_1:50840841-50840860 None:intergenic 40.0%
AAGTAGCCGAATTTCAACCA+TGG - chr2_1:50840901-50840920 Msa0210620:intron 40.0%
!!! GTCAATGATTGTTTTGGCCA+TGG + chr2_1:50840921-50840940 None:intergenic 40.0%
! CAAGGGAAATGGACTTCAAT+TGG + chr2_1:50840961-50840980 None:intergenic 40.0%
AATCCAGTTCTCAAGGGAAA+TGG + chr2_1:50840972-50840991 None:intergenic 40.0%
TCTGACAATCCAGTTCTCAA+GGG + chr2_1:50840978-50840997 None:intergenic 40.0%
TTCTGACAATCCAGTTCTCA+AGG + chr2_1:50840979-50840998 None:intergenic 40.0%
! GAAAGTGGAGTGATTTGCAT+TGG + chr2_1:50841182-50841201 None:intergenic 40.0%
AGTGATGCATATCAAGCAAC+TGG - chr2_1:50841278-50841297 Msa0210620:intron 40.0%
TATAAGCATCTTGGTCTGGA+AGG - chr2_1:50841403-50841422 Msa0210620:intron 40.0%
TAATGTGTAGGACCCAAAAG+AGG - chr2_1:50841964-50841983 Msa0210620:CDS 40.0%
AATGTGTAGGACCCAAAAGA+GGG - chr2_1:50841965-50841984 Msa0210620:CDS 40.0%
!! ATGACCATGTTCTAGGATAC+TGG - chr2_1:50842011-50842030 Msa0210620:intron 40.0%
AGGTATGAGAATAAAGGAGC+TGG + chr2_1:50842037-50842056 None:intergenic 40.0%
! AGAGTGCCTCAATGATTGAA+TGG - chr2_1:50842062-50842081 Msa0210620:intron 40.0%
GAGTGCCTCAATGATTGAAT+GGG - chr2_1:50842063-50842082 Msa0210620:intron 40.0%
AGTGCCTCAATGATTGAATG+GGG - chr2_1:50842064-50842083 Msa0210620:intron 40.0%
ACAGCACACATCAACACAAA+TGG - chr2_1:50842117-50842136 Msa0210620:intron 40.0%
CAGCACACATCAACACAAAT+GGG - chr2_1:50842118-50842137 Msa0210620:intron 40.0%
ACATCAACACAAATGGGAAG+TGG - chr2_1:50842124-50842143 Msa0210620:intron 40.0%
! AGTGGACATTTTCCTGATGA+AGG - chr2_1:50842142-50842161 Msa0210620:intron 40.0%
TTAAGGGCTCCAAAAGATCT+TGG - chr2_1:50842220-50842239 Msa0210620:intron 40.0%
! AATGTCAAAACTGGTAGTGC+TGG - chr2_1:50842271-50842290 Msa0210620:intron 40.0%
CTACTTCTATTATGGTGGTC+CGG - chr2_1:50842306-50842325 Msa0210620:intron 40.0%
TACTTCTATTATGGTGGTCC+GGG - chr2_1:50842307-50842326 Msa0210620:intron 40.0%
TAAATGCGGTTGCAATATCG+TGG - chr2_1:50842387-50842406 Msa0210620:CDS 40.0%
! TGTAAGTTGAGCTACCACTT+AGG - chr2_1:50842512-50842531 Msa0210620:intron 40.0%
! TTTTTGTCTGCTCCTATCTC+TGG - chr2_1:50842673-50842692 Msa0210620:intron 40.0%
!! AAAGCTGGTGGGTTGTTAAT+GGG - chr2_1:50842703-50842722 Msa0210620:intron 40.0%
TTGGTTCCTTTCACTTGTGC+TGG - chr2_1:50839292-50839311 Msa0210620:three_prime_UTR 45.0%
!! ACCTTGATGGACTTAACAGG+CGG + chr2_1:50839418-50839437 None:intergenic 45.0%
TTGTGAAAGCAGAGTCCTGA+TGG - chr2_1:50839764-50839783 Msa0210620:CDS 45.0%
ATGGGTTTGTGATGCAGATG+AGG - chr2_1:50839978-50839997 Msa0210620:intron 45.0%
AGGCCAAATTTCCATCCAGA+AGG - chr2_1:50839998-50840017 Msa0210620:intron 45.0%
GGCCAAATTTCCATCCAGAA+GGG - chr2_1:50839999-50840018 Msa0210620:intron 45.0%
!!! GGTTTTGCAGGTTTTGGTTG+AGG + chr2_1:50840214-50840233 None:intergenic 45.0%
! GGTAGAGGTTTTGCAGGTTT+TGG + chr2_1:50840220-50840239 None:intergenic 45.0%
! ATGTCAGGTAGAGGTTTTGC+AGG + chr2_1:50840226-50840245 None:intergenic 45.0%
TGAGTCAAGATGTCAGGTAG+AGG + chr2_1:50840235-50840254 None:intergenic 45.0%
TGGACCGGCATAACAAATCA+TGG + chr2_1:50840427-50840446 None:intergenic 45.0%
AAAAAAGGACCAGACCTCTG+TGG + chr2_1:50840524-50840543 None:intergenic 45.0%
CATGCCATAGCTTATGTGGA+GGG - chr2_1:50840654-50840673 Msa0210620:intron 45.0%
CAAAGGCGACCATAAACGTT+TGG - chr2_1:50840694-50840713 Msa0210620:intron 45.0%
GCTGTTGAGATCTTGACCAA+AGG + chr2_1:50840781-50840800 None:intergenic 45.0%
! GATCTCAACAGCATTGAAGC+AGG - chr2_1:50840789-50840808 Msa0210620:intron 45.0%
TGGTTAATGGCCTCCAGTTA+AGG - chr2_1:50840865-50840884 Msa0210620:intron 45.0%
!! ATCGATTCGACCTTAACTGG+AGG + chr2_1:50840878-50840897 None:intergenic 45.0%
AGTCCATTTCCCTTGAGAAC+TGG - chr2_1:50840966-50840985 Msa0210620:CDS 45.0%
! TAGGTCAGCCCTGATTTGTA+TGG - chr2_1:50841114-50841133 Msa0210620:intron 45.0%
TGGGGATATACTTGCTCCAA+GGG + chr2_1:50841354-50841373 None:intergenic 45.0%
AATTTGACATCGGAGTCGTC+TGG - chr2_1:50841757-50841776 Msa0210620:CDS 45.0%
! TCCACCAGTTTCCCTCTTTT+GGG + chr2_1:50841979-50841998 None:intergenic 45.0%
! ATCCACCAGTTTCCCTCTTT+TGG + chr2_1:50841980-50841999 None:intergenic 45.0%
! GGAAACTGGTGGATGCAATT+TGG - chr2_1:50841986-50842005 Msa0210620:intron 45.0%
! TCATTGAGGCACTCTCAGTT+AGG + chr2_1:50842057-50842076 None:intergenic 45.0%
ACCACCCCATTCAATCATTG+AGG + chr2_1:50842071-50842090 None:intergenic 45.0%
GTAGTGAACTCTGAGTCTGA+TGG - chr2_1:50842094-50842113 Msa0210620:intron 45.0%
!! TCCTGATGAAGGTTTTGGCA+AGG - chr2_1:50842153-50842172 Msa0210620:intron 45.0%
!! AAACTGGTAGTGCTGGTGAT+TGG - chr2_1:50842278-50842297 Msa0210620:intron 45.0%
!! AACTGGTAGTGCTGGTGATT+GGG - chr2_1:50842279-50842298 Msa0210620:intron 45.0%
! TGGGGCACCTACTTCTATTA+TGG - chr2_1:50842298-50842317 Msa0210620:intron 45.0%
GGCACCTACTTCTATTATGG+TGG - chr2_1:50842301-50842320 Msa0210620:intron 45.0%
CGGACCACCATAATAGAAGT+AGG + chr2_1:50842308-50842327 None:intergenic 45.0%
GAGCTACCACTTAGGTAATG+TGG - chr2_1:50842520-50842539 Msa0210620:intron 45.0%
TTGTTGGAGAAAGAGGGTGA+TGG - chr2_1:50842584-50842603 Msa0210620:intron 45.0%
TGTTGGAGAAAGAGGGTGAT+GGG - chr2_1:50842585-50842604 Msa0210620:intron 45.0%
!! GAAAGCTGGTGGGTTGTTAA+TGG - chr2_1:50842702-50842721 Msa0210620:intron 45.0%
!!! AAAAAATAACATTTTAAAAA+GGG + chr2_1:50841434-50841453 None:intergenic 5.0%
!! AAAAAAATATAAAAAAAACT+AGG - chr2_1:50842460-50842479 Msa0210620:CDS 5.0%
!! AAAAAATATAAAAAAAACTA+GGG - chr2_1:50842461-50842480 Msa0210620:CDS 5.0%
!!! ATATGTATATTTATTTATTT+TGG - chr2_1:50842759-50842778 Msa0210620:five_prime_UTR 5.0%
!! GTTTTGTTGGTGGGGTGCAT+AGG - chr2_1:50838962-50838981 Msa0210620:three_prime_UTR 50.0%
ACCGCCTGTTAAGTCCATCA+AGG - chr2_1:50839414-50839433 Msa0210620:exon 50.0%
CAGGGTGATCAAAAGCTGGT+TGG + chr2_1:50839820-50839839 None:intergenic 50.0%
AGGTCAGGGTGATCAAAAGC+TGG + chr2_1:50839824-50839843 None:intergenic 50.0%
CCACTCTGAGTCAAGATGTC+AGG + chr2_1:50840241-50840260 None:intergenic 50.0%
CCTGACATCTTGACTCAGAG+TGG - chr2_1:50840238-50840257 Msa0210620:intron 50.0%
CTTGACTCAGAGTGGTCATC+AGG - chr2_1:50840246-50840265 Msa0210620:intron 50.0%
!! ATGACTTGTTCACGCCACAG+AGG - chr2_1:50840507-50840526 Msa0210620:intron 50.0%
GCATGCCATAGCTTATGTGG+AGG - chr2_1:50840653-50840672 Msa0210620:intron 50.0%
ACCTCCCTCCACATAAGCTA+TGG + chr2_1:50840661-50840680 None:intergenic 50.0%
GTGGAGGGAGGTGAATTCTA+TGG - chr2_1:50840669-50840688 Msa0210620:intron 50.0%
!!! TGGATTTTGGCTGGTGCCTT+TGG - chr2_1:50840762-50840781 Msa0210620:intron 50.0%
! TCAACAGCATTGAAGCAGGC+TGG - chr2_1:50840793-50840812 Msa0210620:intron 50.0%
!! GCGATCGATTCGACCTTAAC+TGG + chr2_1:50840881-50840900 None:intergenic 50.0%
GTGGGGATATACTTGCTCCA+AGG + chr2_1:50841355-50841374 None:intergenic 50.0%
TGCATGGATTCTATGACGGC+AGG - chr2_1:50841816-50841835 Msa0210620:intron 50.0%
AGGACCCAAAAGAGGGAAAC+TGG - chr2_1:50841972-50841991 Msa0210620:CDS 50.0%
ACCCAAAAGAGGGAAACTGG+TGG - chr2_1:50841975-50841994 Msa0210620:CDS 50.0%
CTGGCCAGTATCCTAGAACA+TGG + chr2_1:50842018-50842037 None:intergenic 50.0%
GCCTCAATGATTGAATGGGG+TGG - chr2_1:50842067-50842086 Msa0210620:intron 50.0%
GCCTTGCCAAAACCTTCATC+AGG + chr2_1:50842157-50842176 None:intergenic 50.0%
!! ACTGGTAGTGCTGGTGATTG+GGG - chr2_1:50842280-50842299 Msa0210620:intron 50.0%
GCTTTCACAGCTCCAGAGAT+AGG + chr2_1:50842688-50842707 None:intergenic 50.0%
!! ATCTCTGGAGCTGTGAAAGC+TGG - chr2_1:50842688-50842707 Msa0210620:intron 50.0%
GCTCTGCCAGCACAAGTGAA+AGG + chr2_1:50839301-50839320 None:intergenic 55.0%
TTGTTCACGCCACAGAGGTC+TGG - chr2_1:50840512-50840531 Msa0210620:intron 55.0%
GCCATAGCTTATGTGGAGGG+AGG - chr2_1:50840657-50840676 Msa0210620:intron 55.0%
GGACAGTTAGGGTTTCGACC+CGG + chr2_1:50842328-50842347 None:intergenic 55.0%
CAATATCGTGGCCGCAGTTG+TGG - chr2_1:50842399-50842418 Msa0210620:CDS 55.0%
! TCTGGAGCTGTGAAAGCTGG+TGG - chr2_1:50842691-50842710 Msa0210620:intron 55.0%
CTGGAGCTGTGAAAGCTGGT+GGG - chr2_1:50842692-50842711 Msa0210620:intron 55.0%
ATCAGCACCACCACCGGGAA+CGG - chr2_1:50839325-50839344 Msa0210620:intron 60.0%
! CAGCATTGAAGCAGGCTGGC+AGG - chr2_1:50840797-50840816 Msa0210620:intron 60.0%
GCCACCATCAGCACCACCAC+CGG - chr2_1:50839319-50839338 Msa0210620:three_prime_UTR 65.0%
CCACCATCAGCACCACCACC+GGG - chr2_1:50839320-50839339 Msa0210620:three_prime_UTR 65.0%
! GTTCCCGGTGGTGGTGCTGA+TGG + chr2_1:50839326-50839345 None:intergenic 65.0%
GCTCTCACCACCACCGTTCC+CGG + chr2_1:50839341-50839360 None:intergenic 65.0%
! GGGAACGGTGGTGGTGAGAG+CGG - chr2_1:50839340-50839359 Msa0210620:intron 65.0%
! AACGGTGGTGGTGAGAGCGG+TGG - chr2_1:50839343-50839362 Msa0210620:intron 65.0%
!! CCCGGTGGTGGTGCTGATGG+TGG + chr2_1:50839323-50839342 None:intergenic 70.0%
AGCACCACCACCGGGAACGG+TGG - chr2_1:50839328-50839347 Msa0210620:intron 70.0%
ACCACCACCGGGAACGGTGG+TGG - chr2_1:50839331-50839350 Msa0210620:intron 70.0%
ACCACCACCGTTCCCGGTGG+TGG + chr2_1:50839335-50839354 None:intergenic 70.0%
CTCACCACCACCGTTCCCGG+TGG + chr2_1:50839338-50839357 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 50838935 50842836 50838935 ID=Msa0210620;Name=Msa0210620
chr2_1 mRNA 50838935 50842836 50838935 ID=Msa0210620-mRNA-1;Parent=Msa0210620;Name=Msa0210620-mRNA-1;_AED=0.17;_eAED=0.17;_QI=90|1|1|1|0.75|0.55|9|416|429
chr2_1 exon 50838935 50839341 50838935 ID=Msa0210620-mRNA-1:exon:14108;Parent=Msa0210620-mRNA-1
chr2_1 exon 50839411 50839797 50839411 ID=Msa0210620-mRNA-1:exon:14107;Parent=Msa0210620-mRNA-1
chr2_1 exon 50840347 50840493 50840347 ID=Msa0210620-mRNA-1:exon:14106;Parent=Msa0210620-mRNA-1
chr2_1 exon 50840601 50840654 50840601 ID=Msa0210620-mRNA-1:exon:14105;Parent=Msa0210620-mRNA-1
chr2_1 exon 50840953 50841117 50840953 ID=Msa0210620-mRNA-1:exon:14104;Parent=Msa0210620-mRNA-1
chr2_1 exon 50841504 50841776 50841504 ID=Msa0210620-mRNA-1:exon:14103;Parent=Msa0210620-mRNA-1
chr2_1 exon 50841909 50841995 50841909 ID=Msa0210620-mRNA-1:exon:14102;Parent=Msa0210620-mRNA-1
chr2_1 exon 50842336 50842526 50842336 ID=Msa0210620-mRNA-1:exon:14101;Parent=Msa0210620-mRNA-1
chr2_1 exon 50842752 50842836 50842752 ID=Msa0210620-mRNA-1:exon:14100;Parent=Msa0210620-mRNA-1
chr2_1 five_prime_UTR 50842752 50842836 50842752 ID=Msa0210620-mRNA-1:five_prime_utr;Parent=Msa0210620-mRNA-1
chr2_1 five_prime_UTR 50842522 50842526 50842522 ID=Msa0210620-mRNA-1:five_prime_utr;Parent=Msa0210620-mRNA-1
chr2_1 CDS 50842336 50842521 50842336 ID=Msa0210620-mRNA-1:cds;Parent=Msa0210620-mRNA-1
chr2_1 CDS 50841909 50841995 50841909 ID=Msa0210620-mRNA-1:cds;Parent=Msa0210620-mRNA-1
chr2_1 CDS 50841504 50841776 50841504 ID=Msa0210620-mRNA-1:cds;Parent=Msa0210620-mRNA-1
chr2_1 CDS 50840953 50841117 50840953 ID=Msa0210620-mRNA-1:cds;Parent=Msa0210620-mRNA-1
chr2_1 CDS 50840601 50840654 50840601 ID=Msa0210620-mRNA-1:cds;Parent=Msa0210620-mRNA-1
chr2_1 CDS 50840347 50840493 50840347 ID=Msa0210620-mRNA-1:cds;Parent=Msa0210620-mRNA-1
chr2_1 CDS 50839420 50839797 50839420 ID=Msa0210620-mRNA-1:cds;Parent=Msa0210620-mRNA-1
chr2_1 three_prime_UTR 50839411 50839419 50839411 ID=Msa0210620-mRNA-1:three_prime_utr;Parent=Msa0210620-mRNA-1
chr2_1 three_prime_UTR 50838935 50839341 50838935 ID=Msa0210620-mRNA-1:three_prime_utr;Parent=Msa0210620-mRNA-1
Gene Sequence

>Msa0210620

ATGGAGGCCAATTTTGGATTATGCTTCTGCTTGTTGGTTTTGTTGGTTCCTTTCACTTGTGCTGGCAGAGCCACCATCAGCACCACCACCGGGAACGGTGGTGGTGAGAGCGGTGGTTCAATTCAGAAACTTGAGGTTAAGAAGCACTTGAAGAATTTGAACAGACCGCCTGTTAAGTCCATCAAGAGTCCTGATGGAGATATTATTGACTGTGTTCATGTTTCACACCAACCAGCTTTTGATCACCCTGACCTCAAAGATCACAAGATTCAGATGAGGCCAAATTTCCATCCAGAAGGGAAAACTTTTGGAGAGAGCAAAGTCTCTTCAACTTCAAATTCAAATTCAAATTCAAAGCCTATAACTCAGCTTTGGCAGAAAAATGGTATTTGTTCTGAAGGAACAATTCCTATAAGAAGGACCAGAACAAGTGACATATTAAGGGCTAGCTCTGTTCAAAATTTTGGAAAGAAGAAGCATAAAAGTACTCCTCAACCAAAACCTGCAAAACCTCTACCTGACATCTTGACTCAGAGTGGTCATCAGCATGCCATAGCTTATGTGGAGGGAGGTGAATTCTATGGAGCAAAGGCGACCATAAACGTTTGGGACCCAAAAATTCAGCAACCTAATGAATTCAGTCTGTCTCAAATTTGGATTTTGGCTGGTGCCTTTGGTCAAGATCTCAACAGCATTGAAGCAGGCTGGCAGGTCAGCCCTGATTTGTATGGAGACAATAACACTAGACTCTTCACTTATTGGACAAGTGATGCATATCAAGCAACTGGTTGTTATAATCTCCTTTGTTCTGGCTTTATTCAAATTAACAATGGTATAGCCCTTGGAGCAAGTATATCCCCACTTTCTAACTATGGTTCTTCCCAATATGATATAAGCATCTTGGTCTGGAAGGACCCAAAAGAGGGAAACTGGTGGATGCAATTTGGAAATGACCATGTTCTAGGATACTGGCCAGCTCCTTTATTCTCATACCTAACTGAGAGTGCCTCAATGATTGAATGGGGTGGTGAAGTAGTGAACTCTGAGTCTGATGGACAGCACACATCAACACAAATGGGAAGTGGACATTTTCCTGATGAAGGTTTTGGCAAGGCTAGTTATTTCAAAAATATTCAGGTTGTTGATGGTGACAACAAGTTAAGGGCTCCAAAAGATCTTGGTACTTACACTGAAAAAGATAACTGTTATAATGTCAAAACTGGTAGTGCTGGTGATTGGGGCACCTACTTCTATTATGGTGGTCCGGGTCGAAACCCTAACTGTCCTTGA
Protein sequence

>Msa0210620

MEANFGLCFCLLVLLVPFTCAGRATISTTTGNGGGESGGSIQKLEVKKHLKNLNRPPVKSIKSPDGDIIDCVHVSHQPAFDHPDLKDHKIQMRPNFHPEGKTFGESKVSSTSNSNSNSKPITQLWQKNGICSEGTIPIRRTRTSDILRASSVQNFGKKKHKSTPQPKPAKPLPDILTQSGHQHAIAYVEGGEFYGAKATINVWDPKIQQPNEFSLSQIWILAGAFGQDLNSIEAGWQVSPDLYGDNNTRLFTYWTSDAYQATGCYNLLCSGFIQINNGIALGASISPLSNYGSSQYDISILVWKDPKEGNWWMQFGNDHVLGYWPAPLFSYLTESASMIEWGGEVVNSESDGQHTSTQMGSGHFPDEGFGKASYFKNIQVVDGDNKLRAPKDLGTYTEKDNCYNVKTGSAGDWGTYFYYGGPGRNPNCP*