AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0289950

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0289950 MtrunA17_Chr2g0309251 97.436 429 8 2 1 429 1 426 0.0 863
Msa0289950 MtrunA17_Chr8g0380511 72.340 423 98 5 7 428 11 415 0.0 629
Msa0289950 MtrunA17_Chr7g0274951 54.374 423 187 4 12 428 17 439 1.56e-171 488
Msa0289950 MtrunA17_Chr1g0189961 52.874 435 186 7 9 429 22 451 6.90e-169 481
Msa0289950 MtrunA17_Chr3g0117271 53.808 407 164 7 25 429 112 496 3.07e-155 448
Msa0289950 MtrunA17_Chr8g0368161 54.124 388 155 3 43 429 83 448 2.78e-154 444
Msa0289950 MtrunA17_Chr4g0058771 54.847 392 154 7 40 429 111 481 1.21e-152 441
Msa0289950 MtrunA17_Chr5g0413131 53.351 388 152 5 46 429 101 463 2.65e-145 422
Msa0289950 MtrunA17_Chr2g0314461 48.082 391 169 7 43 429 46 406 3.39e-124 366
Msa0289950 MtrunA17_Chr2g0314451 48.205 390 174 8 41 429 43 405 4.50e-124 365
Msa0289950 MtrunA17_Chr8g0368241 46.173 392 180 5 43 429 49 414 4.37e-122 361
Msa0289950 MtrunA17_Chr2g0314471 47.423 388 176 7 43 429 46 406 6.18e-122 360
Msa0289950 MtrunA17_Chr3g0135381 41.944 391 211 4 45 426 51 434 4.78e-105 319
Msa0289950 MtrunA17_Chr8g0368201 48.148 324 139 6 123 429 9 320 1.88e-103 310
Msa0289950 MtrunA17_Chr6g0453411 41.534 378 201 8 62 429 8 375 6.71e-103 310
Msa0289950 MtrunA17_Chr3g0135371 39.695 393 221 6 45 429 51 435 3.88e-99 303
Msa0289950 MtrunA17_Chr8g0368291 39.394 429 212 12 12 429 14 405 3.27e-96 294
Msa0289950 MtrunA17_Chr2g0326371 49.835 303 130 8 132 429 75 360 6.56e-90 276
Msa0289950 MtrunA17_Chr8g0368341 40.650 369 180 8 63 429 4 335 4.22e-87 268
Msa0289950 MtrunA17_Chr3g0135401 39.521 334 188 4 105 426 16 347 1.26e-80 252
Msa0289950 MtrunA17_Chr8g0368181 58.031 193 80 1 237 429 2 193 7.21e-78 239
Msa0289950 MtrunA17_Chr1g0151871 38.961 308 167 4 123 429 40 327 8.47e-75 236
Msa0289950 MtrunA17_Chr8g0368221 46.094 256 110 6 50 304 30 258 3.71e-71 225
Msa0289950 MtrunA17_Chr8g0368361 51.282 195 92 3 237 429 2 195 5.04e-70 219
Msa0289950 MtrunA17_Chr3g0129891 50.971 206 93 3 225 428 180 379 7.33e-69 223
Msa0289950 MtrunA17_Chr3g0129891 44.615 130 53 4 43 165 79 196 1.87e-19 89.7
Msa0289950 MtrunA17_Chr8g0368331 41.877 277 129 4 182 429 13 286 8.53e-67 214
Msa0289950 MtrunA17_Chr8g0368281 46.632 193 102 1 237 428 2 194 1.18e-57 187
Msa0289950 MtrunA17_Chr3g0135431 33.611 360 152 9 36 321 35 381 4.26e-55 188
Msa0289950 MtrunA17_Chr3g0138811 32.353 374 206 15 59 425 53 386 3.53e-51 177
Msa0289950 MtrunA17_Chr3g0135441 36.626 243 131 5 190 429 10 232 4.03e-51 172
Msa0289950 MtrunA17_Chr8g0368351 41.379 232 102 8 11 238 47 248 3.01e-45 157
Msa0289950 MtrunA17_Chr8g0368261 57.480 127 52 2 304 429 4 129 5.11e-45 152
Msa0289950 MtrunA17_Chr2g0302691 30.376 372 211 13 60 423 37 368 3.50e-42 152
Msa0289950 MtrunA17_Chr8g0339871 38.211 246 137 12 192 428 2 241 2.04e-41 147
Msa0289950 MtrunA17_Chr8g0368231 53.175 126 57 2 305 429 8 132 5.41e-41 142
Msa0289950 MtrunA17_Chr8g0391201 36.066 244 141 11 193 428 3 239 1.76e-38 139
Msa0289950 MtrunA17_Chr8g0368381 47.788 113 59 0 237 349 2 114 6.92e-38 134
Msa0289950 MtrunA17_Chr5g0445181 32.130 277 150 11 90 366 61 299 4.33e-33 126
Msa0289950 MtrunA17_Chr3g0135451 44.697 132 65 2 35 158 28 159 7.63e-29 112
Msa0289950 MtrunA17_Chr8g0368171 32.068 237 116 5 5 238 10 204 2.70e-27 108
Msa0289950 MtrunA17_Chr8g0353931 30.660 212 113 7 33 238 20 203 1.23e-23 98.2
Msa0289950 MtrunA17_Chr2g0302651 30.942 223 122 11 215 428 1 200 3.55e-22 94.0
Msa0289950 MtrunA17_Chr8g0353941 36.022 186 109 7 244 425 3 182 9.76e-22 92.0
Msa0289950 MtrunA17_Chr5g0426971 28.390 236 142 7 190 423 46 256 4.87e-21 92.0
Msa0289950 MtrunA17_Chr5g0434771 35.849 159 94 5 250 406 12 164 1.39e-20 89.0
Msa0289950 MtrunA17_Chr8g0353961 35.036 137 65 4 31 156 12 135 9.68e-20 85.9
Msa0289950 MtrunA17_Chr8g0353971 32.164 171 108 4 257 423 4 170 1.28e-19 85.9
Msa0289950 MtrunA17_Chr8g0368321 41.176 119 52 4 41 158 41 142 8.44e-18 80.9
Msa0289950 MtrunA17_Chr8g0368271 33.548 155 86 6 9 158 11 153 9.32e-17 78.6
Msa0289950 MtrunA17_Chr5g0443801 36.697 109 67 2 192 300 21 127 2.17e-15 73.9
Msa0289950 MtrunA17_Chr3g0135411 61.224 49 19 0 45 93 64 112 6.45e-14 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0289950 AT3G13510.1 70.396 429 109 5 1 429 9 419 0.0 625
Msa0289950 AT1G55360.1 70.862 429 107 5 1 429 12 422 0.0 622
Msa0289950 AT5G56530.2 69.811 424 109 5 5 428 15 419 0.0 615
Msa0289950 AT5G56530.1 69.811 424 109 5 5 428 15 419 0.0 615
Msa0289950 AT2G44210.1 68.434 396 119 5 35 429 25 415 0.0 576
Msa0289950 AT2G44210.2 63.615 426 119 6 35 429 25 445 0.0 561
Msa0289950 AT5G18460.1 58.673 392 153 6 40 428 44 429 2.37e-164 469
Msa0289950 AT2G44220.1 51.928 389 170 4 42 429 31 403 5.21e-157 449
Msa0289950 AT1G10750.1 55.243 391 151 5 41 429 99 467 1.06e-154 446
Msa0289950 AT5G50150.1 53.964 391 157 5 41 429 51 420 6.94e-153 440
Msa0289950 AT1G23340.2 50.116 431 191 6 1 429 1 409 1.86e-151 436
Msa0289950 AT1G23340.1 50.116 431 191 6 1 429 1 409 1.86e-151 436
Msa0289950 AT1G70550.1 53.964 391 156 6 41 429 97 465 2.82e-149 432
Msa0289950 AT2G44240.1 47.897 428 192 7 3 429 5 402 1.21e-140 408
Msa0289950 AT2G17750.1 44.762 420 202 7 9 428 6 395 4.98e-133 388
Msa0289950 AT2G19360.1 46.189 433 199 7 14 428 9 425 7.56e-131 384
Msa0289950 AT2G44250.1 44.419 430 203 10 5 428 9 408 3.86e-121 358
Msa0289950 AT3G48230.1 43.669 387 187 11 50 429 11 373 1.05e-109 328
Msa0289950 AT5G25950.1 40.842 404 208 9 44 429 42 432 1.14e-93 289
Msa0289950 AT1G23340.3 48.366 306 134 6 1 304 1 284 3.01e-93 283
Msa0289950 AT5G19170.1 40.000 390 201 10 40 423 3 365 4.08e-93 286
Msa0289950 AT2G44250.2 45.833 312 159 7 121 428 20 325 1.61e-91 280
Msa0289950 AT2G20170.1 39.075 389 206 13 42 423 32 396 9.33e-85 265
Msa0289950 AT2G20170.2 39.075 389 206 13 42 423 32 396 9.33e-85 265
Msa0289950 AT5G25960.1 39.075 389 180 8 44 429 23 357 1.03e-81 256
Msa0289950 AT2G20170.3 35.990 389 201 13 42 423 32 379 3.87e-73 234
Msa0289950 AT4G23370.1 35.417 384 216 13 50 423 40 401 5.99e-70 227
Msa0289950 AT4G23355.1 35.696 395 210 14 42 423 35 398 1.15e-68 223
Msa0289950 AT4G23350.1 33.419 389 229 10 42 423 35 400 2.28e-67 220
Msa0289950 AT4G23390.1 35.509 383 212 14 50 423 41 397 6.42e-66 216
Msa0289950 AT4G23365.1 33.085 402 211 14 42 423 35 398 6.87e-66 216
Msa0289950 AT4G23360.1 34.097 393 220 12 42 423 35 399 3.07e-64 212
Msa0289950 AT4G23380.1 32.143 392 227 11 42 423 36 398 3.03e-62 206
Msa0289950 AT1G70550.2 49.770 217 85 6 41 255 97 291 1.23e-60 199
Msa0289950 AT2G35250.1 35.440 364 182 12 67 425 24 339 1.28e-54 185
Msa0289950 AT1G10750.2 42.797 236 105 7 46 276 105 315 9.33e-53 180
Msa0289950 AT1G10190.1 33.085 402 222 18 35 423 27 394 1.70e-49 173
Msa0289950 AT4G15050.1 33.333 405 217 19 35 423 27 394 1.73e-47 167
Msa0289950 AT4G15053.1 31.378 392 231 15 42 423 38 401 1.86e-46 165
Msa0289950 AT5G46810.1 30.000 370 212 13 70 423 11 349 1.72e-43 156
Msa0289950 AT2G27320.1 33.016 315 161 10 69 380 30 297 5.95e-41 148
Msa0289950 AT4G17505.1 29.032 372 193 10 58 426 18 321 1.11e-40 147
Msa0289950 AT5G46820.1 27.532 385 221 13 55 423 5 347 1.34e-40 148
Msa0289950 AT5G05030.1 30.287 383 211 18 54 425 22 359 3.52e-39 144
Msa0289950 AT2G24950.1 28.972 428 249 19 8 423 25 409 3.94e-39 145
Msa0289950 AT4G10220.1 29.008 393 230 16 42 423 44 398 1.45e-38 144
Msa0289950 AT4G23373.1 32.319 263 131 9 13 274 7 223 1.84e-38 141
Msa0289950 AT5G25410.1 26.862 376 229 12 54 423 28 363 1.58e-36 137
Msa0289950 AT5G60380.1 27.778 378 221 14 57 423 31 367 5.49e-35 133
Msa0289950 AT5G46200.1 27.110 391 232 13 70 423 31 405 5.55e-35 134
Msa0289950 AT4G10210.1 27.857 420 251 16 1 406 3 384 2.48e-32 127
Msa0289950 AT4G17860.1 28.378 370 202 16 70 423 29 351 4.08e-32 125
Msa0289950 AT4G23080.1 27.064 436 262 17 10 425 7 406 1.55e-30 122
Msa0289950 AT2G38255.1 33.333 225 105 7 70 292 14 195 1.71e-29 114
Msa0289950 AT5G11660.1 30.368 326 188 14 114 425 4 304 1.56e-28 114
Msa0289950 AT5G25415.1 27.219 338 205 13 105 425 19 332 4.73e-23 99.8
Msa0289950 AT2G03930.2 42.105 114 62 3 170 281 31 142 7.51e-23 95.1
Msa0289950 AT5G36680.1 27.213 305 181 11 131 423 52 327 9.96e-20 90.1
Msa0289950 AT5G36770.1 27.213 305 181 11 131 423 52 327 9.96e-20 90.1
Msa0289950 AT5G37520.1 32.328 232 116 13 199 424 2 198 6.00e-19 85.1
Msa0289950 AT5G04937.1 30.000 210 120 7 221 423 9 198 2.89e-18 83.2

Find 145 sgRNAs with CRISPR-Local

Find 277 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTAGCTCTGTTCAAAATTT+TGG 0.058994 2_3:+36545366 Msa0289950:CDS
ATCCACCAGTTTCCCTCTTT+TGG 0.121092 2_3:-36547171 None:intergenic
AATTCAGTCTGTCTCAAATT+TGG 0.126548 2_3:+36545938 Msa0289950:CDS
TTCCCTTCTGGATGGAAATT+TGG 0.152045 2_3:-36545201 None:intergenic
ATATGTATATTTATTTATTT+TGG 0.152989 2_3:+36547953 Msa0289950:three_prime_UTR
ACACTAGACTCTTCACTTAT+TGG 0.158217 2_3:+36546333 Msa0289950:CDS
CATCCAGAAGGGAAAACTTT+TGG 0.204617 2_3:+36545210 Msa0289950:CDS
GCTTTAATGGAGGCCAATTT+TGG 0.214424 2_3:+36544444 Msa0289950:intron
CATAAATAATGTTCTTATTA+AGG 0.219963 2_3:-36548002 None:intergenic
TCTGTCTCAAATTTGGATTT+TGG 0.224441 2_3:+36545945 Msa0289950:CDS
CCAGAACAAGTGACATATTA+AGG 0.229209 2_3:+36545343 Msa0289950:CDS
CATAGGATAAGCTTCAAAAT+TGG 0.235823 2_3:+36544179 Msa0289950:five_prime_UTR
TTCTGCCAAAGCTGAGTTAT+AGG 0.242996 2_3:-36545279 None:intergenic
TGTTATAATCTCCTTTGTTC+TGG 0.263241 2_3:+36546487 Msa0289950:CDS
TGCATTTGGAGAAGCTTTAA+TGG 0.266793 2_3:+36544431 Msa0289950:intron
CCTTAATATGTCACTTGTTC+TGG 0.271266 2_3:-36545343 None:intergenic
CTTGTTCTGGTCCTTCTTAT+AGG 0.284686 2_3:-36545330 None:intergenic
TTTGGAAATGACCATGTTCT+AGG 0.286569 2_3:+36547198 Msa0289950:CDS
CCAAGATGCTTATATCATAT+TGG 0.295235 2_3:-36546579 None:intergenic
CCTGAATCTTGTGATCTTTG+AGG 0.299817 2_3:-36545041 None:intergenic
GCAGAAGCATAATCCAAAAT+TGG 0.311137 2_3:-36544457 None:intergenic
TGGATTATGCTTCTGCTTGT+TGG 0.325487 2_3:+36544464 Msa0289950:CDS
TGGGGCACCTACTTCTATTA+TGG 0.331136 2_3:+36547492 Msa0289950:CDS
GGAAACTGGTGGATGCAATT+TGG 0.341439 2_3:+36547180 Msa0289950:CDS
ATAAAAGATCAAGGACAGTT+AGG 0.341879 2_3:-36547531 None:intergenic
TGAGACAGACTGAATTCATT+AGG 0.345968 2_3:-36545931 None:intergenic
ATATCCCCACTTTCTAACTA+TGG 0.346949 2_3:+36546550 Msa0289950:CDS
AACTGGTAGTGCTGGTGATT+GGG 0.351854 2_3:+36547473 Msa0289950:CDS
AAAAGTTATCTCTACCTTGA+TGG 0.356984 2_3:-36544628 None:intergenic
CCTCAAAGATCACAAGATTC+AGG 0.363603 2_3:+36545041 Msa0289950:CDS
ATCTTGTGATCTTTGAGGTC+AGG 0.364868 2_3:-36545036 None:intergenic
GTGTAAGTACCAAGATCTTT+TGG 0.369659 2_3:-36547423 None:intergenic
CAAGATGCTTATATCATATT+GGG 0.373261 2_3:-36546578 None:intergenic
GCTCTCACCACCACCGTTCC+CGG 0.378518 2_3:-36544538 None:intergenic
GAGCTACCACTTAGGTAATA+TGG 0.378586 2_3:+36547714 Msa0289950:three_prime_UTR
TTATTGTCACCGTACAAATC+AGG 0.381973 2_3:-36546311 None:intergenic
AACTGTTATAATGTCAAAAC+TGG 0.390356 2_3:+36547456 Msa0289950:CDS
TGAACAGAATATTCTCTAGT+AGG 0.392533 2_3:+36547918 Msa0289950:three_prime_UTR
TTGGTTCCTTTCACTTGTGC+TGG 0.399660 2_3:+36544492 Msa0289950:CDS
GCTTTCACAGCTCCAGAGAT+AGG 0.400467 2_3:-36547879 None:intergenic
CTACTTCTATTATGGTGGTC+CGG 0.402420 2_3:+36547500 Msa0289950:CDS
TAAGCTTCAAAATTGGGTTA+AGG 0.406474 2_3:+36544186 Msa0289950:five_prime_UTR
GTGGAGGGAGGTGAATTCTA+TGG 0.407436 2_3:+36545865 Msa0289950:CDS
ATAGGATAAGCTTCAAAATT+GGG 0.416322 2_3:+36544180 Msa0289950:five_prime_UTR
CAAAGGCGACCATAAACGTT+TGG 0.418700 2_3:+36545890 Msa0289950:CDS
TTGATGGTGACAACAAGTTA+AGG 0.430415 2_3:+36547397 Msa0289950:CDS
GCCACCATCAGCACCACCAC+CGG 0.431095 2_3:+36544519 Msa0289950:CDS
TCTACCTTGATGGACTTAAC+AGG 0.434566 2_3:-36544618 None:intergenic
ATGACCATGTTCTAGGATAC+TGG 0.437458 2_3:+36547205 Msa0289950:CDS
CAAAGCCTATAACTCAGCTT+TGG 0.444736 2_3:+36545274 Msa0289950:CDS
ACCACCACCGTTCCCGGTGG+TGG 0.447471 2_3:-36544532 None:intergenic
TAAAAGATCAAGGACAGTTA+GGG 0.448907 2_3:-36547530 None:intergenic
ACAGCACACATCAACACAAA+TGG 0.449226 2_3:+36547311 Msa0289950:CDS
CCAATATGATATAAGCATCT+TGG 0.452731 2_3:+36546579 Msa0289950:CDS
AAACTGGTAGTGCTGGTGAT+TGG 0.461992 2_3:+36547472 Msa0289950:CDS
AGGACCCAAAAGAGGGAAAC+TGG 0.463817 2_3:+36547166 Msa0289950:intron
TCAGTTAGGTATGAGAATAA+AGG 0.467064 2_3:-36547234 None:intergenic
CTTGACTCAGAGTGGTCATC+AGG 0.468890 2_3:+36545446 Msa0289950:CDS
TGTTGGAGAAAGAGGGTGAT+GGG 0.475386 2_3:+36547779 Msa0289950:three_prime_UTR
ATCTCTGGAGCTGTGAAAGC+TGG 0.479478 2_3:+36547882 Msa0289950:three_prime_UTR
TTAAGGGCACCAAAAGATCT+TGG 0.482227 2_3:+36547414 Msa0289950:CDS
AAAGGCGACCATAAACGTTT+GGG 0.483661 2_3:+36545891 Msa0289950:CDS
TCATTGAGGCACTCTCAGTT+AGG 0.484694 2_3:-36547248 None:intergenic
TACTTCTATTATGGTGGTCC+GGG 0.485729 2_3:+36547501 Msa0289950:CDS
TGATGGTGACAACAAGTTAA+GGG 0.487718 2_3:+36547398 Msa0289950:CDS
ATTAACAATGGTATAGCCCT+TGG 0.488395 2_3:+36546520 Msa0289950:CDS
GCCTTGCCAAAACCTTCATC+AGG 0.488665 2_3:-36547348 None:intergenic
ATGATATAAGCATCTTGGTC+TGG 0.490781 2_3:+36546584 Msa0289950:CDS
AGAGTGCCTCAATGATTGAA+TGG 0.494264 2_3:+36547256 Msa0289950:CDS
TGGATTGCAATTTAAAACTA+CGG 0.494301 2_3:+36547613 Msa0289950:intron
AAGGAACAATTCCTATAAGA+AGG 0.498065 2_3:+36545319 Msa0289950:CDS
ACCACCACCGGGAACGGTGG+TGG 0.505459 2_3:+36544531 Msa0289950:CDS
CAGCATTGAAGCAGGCTGGC+AGG 0.505596 2_3:+36545993 Msa0289950:CDS
GAACAGAATATTCTCTAGTA+GGG 0.506018 2_3:+36547919 Msa0289950:three_prime_UTR
TTGGGTTAAGGTTAAGCCTA+AGG 0.507890 2_3:+36544198 Msa0289950:five_prime_UTR
CCACTCTGAGTCAAGATGTC+AGG 0.518935 2_3:-36545438 None:intergenic
CAGCACACATCAACACAAAT+GGG 0.520852 2_3:+36547312 Msa0289950:CDS
CAGAACAAGTGACATATTAA+GGG 0.521105 2_3:+36545344 Msa0289950:CDS
GAGTGCCTCAATGATTGAAT+GGG 0.527263 2_3:+36547257 Msa0289950:CDS
GTTCCCGGTGGTGGTGCTGA+TGG 0.531155 2_3:-36544523 None:intergenic
TTAGCATGCCATAGCTTATG+TGG 0.531230 2_3:+36545846 Msa0289950:intron
AATGTCAAAACTGGTAGTGC+TGG 0.534590 2_3:+36547465 Msa0289950:CDS
GGCACCTACTTCTATTATGG+TGG 0.539245 2_3:+36547495 Msa0289950:CDS
GATCTCAACAGCATTGAAGC+AGG 0.539985 2_3:+36545985 Msa0289950:CDS
GAAGAACCATAGTTAGAAAG+TGG 0.541399 2_3:-36546556 None:intergenic
TTGTGAAAGCAGAGTCCTGA+TGG 0.541959 2_3:+36544964 Msa0289950:intron
CCACCATCAGCACCACCACC+GGG 0.545767 2_3:+36544520 Msa0289950:CDS
AGGTCAGGGTGATCAAAAGC+TGG 0.547634 2_3:-36545021 None:intergenic
TCTGGAGCTGTGAAAGCTGG+TGG 0.550324 2_3:+36547885 Msa0289950:three_prime_UTR
ACCTCCCTCCACATAAGCTA+TGG 0.557000 2_3:-36545854 None:intergenic
CCCGGTGGTGGTGCTGATGG+TGG 0.557572 2_3:-36544520 None:intergenic
TGTAAGTTGAGCTACCACTT+AGG 0.562344 2_3:+36547706 Msa0289950:three_prime_UTR
GGCTTTATTCAAATTAACAA+TGG 0.564459 2_3:+36546508 Msa0289950:CDS
TGGGGATATACTTGCTCCAA+GGG 0.564591 2_3:-36546536 None:intergenic
ACATCAACACAAATGGGAAG+TGG 0.565243 2_3:+36547318 Msa0289950:CDS
TAATGTGTAGGACCCAAAAG+AGG 0.565462 2_3:+36547158 Msa0289950:intron
CAGTCAATAATATCTCCATC+AGG 0.565977 2_3:-36544979 None:intergenic
TATACACCATATTACCTAAG+TGG 0.566286 2_3:-36547720 None:intergenic
AGCTTTAACATAAAAGATCA+AGG 0.566363 2_3:-36547540 None:intergenic
TAGGTCAGCCCTGATTTGTA+CGG 0.566591 2_3:+36546302 Msa0289950:intron
GGGAACGGTGGTGGTGAGAG+CGG 0.566812 2_3:+36544540 Msa0289950:CDS
TCTTGTGATCTTTGAGGTCA+GGG 0.570285 2_3:-36545035 None:intergenic
AGGTATGAGAATAAAGGAGC+TGG 0.573128 2_3:-36547228 None:intergenic
GAAGCTCAAAATTCTCATTG+AGG 0.576114 2_3:+36547745 Msa0289950:three_prime_UTR
CTGGAGCTGTGAAAGCTGGT+GGG 0.576766 2_3:+36547886 Msa0289950:three_prime_UTR
ACCACCCCATTCAATCATTG+AGG 0.578345 2_3:-36547262 None:intergenic
AACGGTGGTGGTGAGAGCGG+TGG 0.580017 2_3:+36544543 Msa0289950:CDS
GTGGGGATATACTTGCTCCA+AGG 0.581673 2_3:-36546537 None:intergenic
TCAACAGCATTGAAGCAGGC+TGG 0.583391 2_3:+36545989 Msa0289950:CDS
TATTGTCACCGTACAAATCA+GGG 0.584428 2_3:-36546310 None:intergenic
AGGCCAAATTTCCATCCAGA+AGG 0.584529 2_3:+36545198 Msa0289950:CDS
CGGACCACCATAATAGAAGT+AGG 0.584855 2_3:-36547499 None:intergenic
TGGTTCAATTCAGAAACTTG+AGG 0.588867 2_3:+36544563 Msa0289950:CDS
TATAAGCATCTTGGTCTGGA+AGG 0.589005 2_3:+36546588 Msa0289950:CDS
TTGTTGGAGAAAGAGGGTGA+TGG 0.593904 2_3:+36547778 Msa0289950:three_prime_UTR
AAGAACCATAGTTAGAAAGT+GGG 0.595857 2_3:-36546555 None:intergenic
ACTGGTAGTGCTGGTGATTG+GGG 0.595966 2_3:+36547474 Msa0289950:CDS
TTTGAATAAAGCCAGAACAA+AGG 0.597232 2_3:-36546498 None:intergenic
TGAGTCAAGATGTCAGGTAG+AGG 0.600193 2_3:-36545432 None:intergenic
ATCAGCACCACCACCGGGAA+CGG 0.601551 2_3:+36544525 Msa0289950:CDS
GCTCTGCCAGCACAAGTGAA+AGG 0.602569 2_3:-36544498 None:intergenic
CAGGGTGATCAAAAGCTGGT+TGG 0.603523 2_3:-36545017 None:intergenic
AGTGATGCATATCAAGCCAC+TGG 0.605418 2_3:+36546463 Msa0289950:CDS
CTGGCCAGTATCCTAGAACA+TGG 0.608996 2_3:-36547209 None:intergenic
AGCACCACCACCGGGAACGG+TGG 0.613079 2_3:+36544528 Msa0289950:CDS
ACCGCCTGTTAAGTCCATCA+AGG 0.617331 2_3:+36544614 Msa0289950:CDS
AGGTGAATTCTATGGAGCAA+AGG 0.618255 2_3:+36545873 Msa0289950:CDS
AATGTGTAGGACCCAAAAGA+GGG 0.626016 2_3:+36547159 Msa0289950:intron
GTTGTGAACTCTGAATCTGA+TGG 0.627674 2_3:+36547288 Msa0289950:CDS
AACAGAATATTCTCTAGTAG+GGG 0.643070 2_3:+36547920 Msa0289950:three_prime_UTR
GGCCAAATTTCCATCCAGAA+GGG 0.643729 2_3:+36545199 Msa0289950:CDS
CATGCCATAGCTTATGTGGA+GGG 0.644068 2_3:+36545850 Msa0289950:CDS
GCATGCCATAGCTTATGTGG+AGG 0.646750 2_3:+36545849 Msa0289950:intron
AGAACCATAGTTAGAAAGTG+GGG 0.647282 2_3:-36546554 None:intergenic
ATTTGGAGAAGCTTTAATGG+AGG 0.650311 2_3:+36544434 Msa0289950:intron
GCTGTTGAGATCTTGACCAA+AGG 0.656530 2_3:-36545974 None:intergenic
AAGGAGATTATAACAACCAG+TGG 0.657697 2_3:-36546479 None:intergenic
GCCATAGCTTATGTGGAGGG+AGG 0.658878 2_3:+36545853 Msa0289950:CDS
GGACAGTTAGGGTTTCGACC+CGG 0.661025 2_3:-36547519 None:intergenic
GCCTCAATGATTGAATGGGG+TGG 0.671790 2_3:+36547261 Msa0289950:CDS
ACCTTGATGGACTTAACAGG+CGG 0.693538 2_3:-36544615 None:intergenic
CCTGACATCTTGACTCAGAG+TGG 0.701149 2_3:+36545438 Msa0289950:CDS
ACCCAAAAGAGGGAAACTGG+TGG 0.707549 2_3:+36547169 Msa0289950:CDS
CTCACCACCACCGTTCCCGG+TGG 0.716489 2_3:-36544535 None:intergenic
AGTGCCTCAATGATTGAATG+GGG 0.753510 2_3:+36547258 Msa0289950:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAAAATATAGAACTTTTTA+TGG + chr2_3:36544315-36544334 Msa0289950:intron 10.0%
!!! TTGTAAAAAATTAAACTTTT+TGG + chr2_3:36544714-36544733 Msa0289950:intron 10.0%
!!! TGTAAAAAATTAAACTTTTT+GGG + chr2_3:36544715-36544734 Msa0289950:intron 10.0%
!!! ATTTTAGTATGTTTTTATAT+TGG + chr2_3:36544842-36544861 Msa0289950:intron 10.0%
!!! GATTTAATTTTTAGATTTTT+AGG + chr2_3:36544893-36544912 Msa0289950:intron 10.0%
!!! AAAAGATAACATTTTAAAAA+GGG - chr2_3:36546619-36546638 None:intergenic 10.0%
!! TATAGCATATACAATTAAAT+GGG - chr2_3:36544290-36544309 None:intergenic 15.0%
!! TTATAGCATATACAATTAAA+TGG - chr2_3:36544291-36544310 None:intergenic 15.0%
!! TTTCTAAATTCAAACAATTA+TGG + chr2_3:36545159-36545178 Msa0289950:intron 15.0%
!! TTCTAAATTCAAACAATTAT+GGG + chr2_3:36545160-36545179 Msa0289950:intron 15.0%
!! AAATTGGTTATTGAAATTTA+TGG - chr2_3:36545644-36545663 None:intergenic 15.0%
!! AACATATACTATCATAAAAA+AGG - chr2_3:36545735-36545754 None:intergenic 15.0%
!! TAAAACAAATACATTTAATC+AGG - chr2_3:36545797-36545816 None:intergenic 15.0%
!!! CAAAAGATAACATTTTAAAA+AGG - chr2_3:36546620-36546639 None:intergenic 15.0%
!!! AAAATGTTATCTTTTGTTAT+TGG + chr2_3:36546624-36546643 Msa0289950:intron 15.0%
!! ATTGATACATATTAAATTCA+TGG - chr2_3:36547105-36547124 None:intergenic 15.0%
!!! TAGTTATTTCAAAAATATTC+AGG + chr2_3:36547371-36547390 Msa0289950:CDS 15.0%
!! CATAAATAATGTTCTTATTA+AGG - chr2_3:36548005-36548024 None:intergenic 15.0%
!!! TAGAACTTTTTATGGTTTTT+TGG + chr2_3:36544323-36544342 Msa0289950:intron 20.0%
!!! ATAACGGTTTTATTTCTAAT+TGG + chr2_3:36544688-36544707 Msa0289950:intron 20.0%
!!! TAACGGTTTTATTTCTAATT+GGG + chr2_3:36544689-36544708 Msa0289950:intron 20.0%
!! AAATGAACTATGTTTATGAA+TGG - chr2_3:36544920-36544939 None:intergenic 20.0%
!!! ATTTGTTAGTAAACTATTAG+TGG - chr2_3:36545522-36545541 None:intergenic 20.0%
!! TCAATAATTGACGAATAAAT+TGG - chr2_3:36545660-36545679 None:intergenic 20.0%
!!! TCCTTTTGATTTTATTTTCT+TGG + chr2_3:36545818-36545837 Msa0289950:intron 20.0%
!! TCCAAGAAAATAAAATCAAA+AGG - chr2_3:36545822-36545841 None:intergenic 20.0%
!! TTTAAAAGCTAATTTGACAT+CGG + chr2_3:36546940-36546959 Msa0289950:intron 20.0%
!! AAATAACAGCTTAAAATCAT+TGG - chr2_3:36546983-36547002 None:intergenic 20.0%
!! GAATAAAAAAACTAATGTGT+AGG + chr2_3:36547146-36547165 Msa0289950:intron 20.0%
! ATAGGATAAGCTTCAAAATT+GGG + chr2_3:36544180-36544199 Msa0289950:five_prime_UTR 25.0%
! TGTATGTAATATTCTACCTT+AGG - chr2_3:36544217-36544236 None:intergenic 25.0%
! CAAAGACTAATGAAGTTAAA+GGG - chr2_3:36544352-36544371 None:intergenic 25.0%
! ACAAAGACTAATGAAGTTAA+AGG - chr2_3:36544353-36544372 None:intergenic 25.0%
! CTTTATGAGAATTGTCAAAT+GGG - chr2_3:36544394-36544413 None:intergenic 25.0%
! ACTTTATGAGAATTGTCAAA+TGG - chr2_3:36544395-36544414 None:intergenic 25.0%
!!! TTAAACTTTTTGGGTTTGAA+AGG + chr2_3:36544724-36544743 Msa0289950:intron 25.0%
! ATTGTAGTGAAAAAGACTAA+AGG - chr2_3:36544778-36544797 None:intergenic 25.0%
!!! AGTCTTTTTCACTACAATAA+TGG + chr2_3:36544780-36544799 Msa0289950:intron 25.0%
!!! TCTTTCTTTTATGTGTCATT+TGG + chr2_3:36545123-36545142 Msa0289950:intron 25.0%
! CATTCAAATGAAGTAAACAT+AGG - chr2_3:36545556-36545575 None:intergenic 25.0%
! TAGTATATGTTACTATAGCA+TGG + chr2_3:36545744-36545763 Msa0289950:intron 25.0%
!!! CATTAGGTTGTTGAATTTTT+GGG - chr2_3:36545918-36545937 None:intergenic 25.0%
!!! TCATTAGGTTGTTGAATTTT+TGG - chr2_3:36545919-36545938 None:intergenic 25.0%
! TGTATGACTTAATTGGTTAA+TGG + chr2_3:36546050-36546069 Msa0289950:intron 25.0%
!! CGTTATCTTTTGAATGATTT+TGG + chr2_3:36546239-36546258 Msa0289950:intron 25.0%
!! AATTTAATGAAAGCACAAAG+TGG - chr2_3:36546384-36546403 None:intergenic 25.0%
!!! GGCTTTATTCAAATTAACAA+TGG + chr2_3:36546508-36546527 Msa0289950:CDS 25.0%
! CAAGATGCTTATATCATATT+GGG - chr2_3:36546581-36546600 None:intergenic 25.0%
!! GGCATATTTTCTTTCTATAA+TGG - chr2_3:36546650-36546669 None:intergenic 25.0%
! GTAATGTATGAATGTATGTA+TGG + chr2_3:36546795-36546814 Msa0289950:intron 25.0%
! AGTCAACAACTTAAAAGAAT+TGG + chr2_3:36546893-36546912 Msa0289950:intron 25.0%
! AAATTTGAATTCGTACACAT+GGG + chr2_3:36547032-36547051 Msa0289950:intron 25.0%
!!! AAAAATATTCAGGTTGTTGA+TGG + chr2_3:36547381-36547400 Msa0289950:CDS 25.0%
! AACTGTTATAATGTCAAAAC+TGG + chr2_3:36547456-36547475 Msa0289950:CDS 25.0%
!! AGCTTTAACATAAAAGATCA+AGG - chr2_3:36547543-36547562 None:intergenic 25.0%
!! TTTTAAATTGCAGGTAAATG+CGG + chr2_3:36547567-36547586 Msa0289950:intron 25.0%
!! TGGATTGCAATTTAAAACTA+CGG + chr2_3:36547613-36547632 Msa0289950:intron 25.0%
!! AGCTTTTCATTAATCAAACA+TGG - chr2_3:36547981-36548000 None:intergenic 25.0%
!!! GTTGTTGTTGTTTGTTTTGT+TGG + chr2_3:36544149-36544168 Msa0289950:five_prime_UTR 30.0%
!!! TTGTTGTTTGTTTTGTTGGT+GGG + chr2_3:36544153-36544172 Msa0289950:five_prime_UTR 30.0%
CATAGGATAAGCTTCAAAAT+TGG + chr2_3:36544179-36544198 Msa0289950:five_prime_UTR 30.0%
TAAGCTTCAAAATTGGGTTA+AGG + chr2_3:36544186-36544205 Msa0289950:five_prime_UTR 30.0%
AAAAGTTATCTCTACCTTGA+TGG - chr2_3:36544631-36544650 None:intergenic 30.0%
!! CTTTTTCATTGGTTGCATAA+CGG + chr2_3:36544672-36544691 Msa0289950:intron 30.0%
!! TTGAAAGGAACTTTAGTTCT+TGG + chr2_3:36544739-36544758 Msa0289950:intron 30.0%
!! TGAAAGGAACTTTAGTTCTT+GGG + chr2_3:36544740-36544759 Msa0289950:intron 30.0%
TATGCAAGTGTACTTTGTTA+TGG + chr2_3:36544939-36544958 Msa0289950:intron 30.0%
AAAAATGGTATGTGTTCTGA+AGG + chr2_3:36545300-36545319 Msa0289950:CDS 30.0%
AAGGAACAATTCCTATAAGA+AGG + chr2_3:36545319-36545338 Msa0289950:CDS 30.0%
CAGAACAAGTGACATATTAA+GGG + chr2_3:36545344-36545363 Msa0289950:CDS 30.0%
TAGGTGCATTATAAGTAACA+TGG - chr2_3:36545483-36545502 None:intergenic 30.0%
GGTTATTGAAATTTATGGAC+CGG - chr2_3:36545639-36545658 None:intergenic 30.0%
AATTCAGTCTGTCTCAAATT+TGG + chr2_3:36545938-36545957 Msa0289950:CDS 30.0%
! TCTGTCTCAAATTTGGATTT+TGG + chr2_3:36545945-36545964 Msa0289950:CDS 30.0%
CATCTTCTGTATGACTTAAT+TGG + chr2_3:36546043-36546062 Msa0289950:intron 30.0%
!!! CCAATGATTGTTTTTTTCAC+GGG - chr2_3:36546119-36546138 None:intergenic 30.0%
!!! GCCAATGATTGTTTTTTTCA+CGG - chr2_3:36546120-36546139 None:intergenic 30.0%
TGTTATAATCTCCTTTGTTC+TGG + chr2_3:36546487-36546506 Msa0289950:CDS 30.0%
TTTGAATAAAGCCAGAACAA+AGG - chr2_3:36546501-36546520 None:intergenic 30.0%
AAGAACCATAGTTAGAAAGT+GGG - chr2_3:36546558-36546577 None:intergenic 30.0%
CCAAGATGCTTATATCATAT+TGG - chr2_3:36546582-36546601 None:intergenic 30.0%
CCAATATGATATAAGCATCT+TGG + chr2_3:36546579-36546598 Msa0289950:CDS 30.0%
TTGTAGTGTTAGTTAAGTGT+GGG - chr2_3:36546672-36546691 None:intergenic 30.0%
ATTGTAGTGTTAGTTAAGTG+TGG - chr2_3:36546673-36546692 None:intergenic 30.0%
AGAATTGGATGAAAATCAGA+CGG + chr2_3:36546908-36546927 Msa0289950:intron 30.0%
TAAATGCATGGAATCTATGA+CGG + chr2_3:36547005-36547024 Msa0289950:intron 30.0%
GAAATTTGAATTCGTACACA+TGG + chr2_3:36547031-36547050 Msa0289950:intron 30.0%
TCAGTTAGGTATGAGAATAA+AGG - chr2_3:36547237-36547256 None:intergenic 30.0%
TAAAAGATCAAGGACAGTTA+GGG - chr2_3:36547533-36547552 None:intergenic 30.0%
ATAAAAGATCAAGGACAGTT+AGG - chr2_3:36547534-36547553 None:intergenic 30.0%
! GATCTTTTATGTTAAAGCTG+TGG + chr2_3:36547544-36547563 Msa0289950:intron 30.0%
TATACACCATATTACCTAAG+TGG - chr2_3:36547723-36547742 None:intergenic 30.0%
CAAAAATTGTTGGAGAAAGA+GGG + chr2_3:36547772-36547791 Msa0289950:three_prime_UTR 30.0%
TGAACAGAATATTCTCTAGT+AGG + chr2_3:36547918-36547937 Msa0289950:three_prime_UTR 30.0%
GAACAGAATATTCTCTAGTA+GGG + chr2_3:36547919-36547938 Msa0289950:three_prime_UTR 30.0%
AACAGAATATTCTCTAGTAG+GGG + chr2_3:36547920-36547939 Msa0289950:three_prime_UTR 30.0%
!!! GTTGTTGTTTGTTTTGTTGG+TGG + chr2_3:36544152-36544171 Msa0289950:five_prime_UTR 35.0%
!!! TGTTGTTTGTTTTGTTGGTG+GGG + chr2_3:36544154-36544173 Msa0289950:five_prime_UTR 35.0%
! CATGCTTTTGCATTTGCATT+TGG + chr2_3:36544417-36544436 Msa0289950:intron 35.0%
! TGCATTTGGAGAAGCTTTAA+TGG + chr2_3:36544431-36544450 Msa0289950:intron 35.0%
! ATTTGGAGAAGCTTTAATGG+AGG + chr2_3:36544434-36544453 Msa0289950:intron 35.0%
! GCAGAAGCATAATCCAAAAT+TGG - chr2_3:36544460-36544479 None:intergenic 35.0%
TGGTTCAATTCAGAAACTTG+AGG + chr2_3:36544563-36544582 Msa0289950:CDS 35.0%
GAAAAAGTGACAGAGTGAAA+AGG - chr2_3:36544659-36544678 None:intergenic 35.0%
!!! TCACTCTGTCACTTTTTCAT+TGG + chr2_3:36544661-36544680 Msa0289950:intron 35.0%
!! AACTTTAGTTCTTGGGAGTT+GGG + chr2_3:36544747-36544766 Msa0289950:intron 35.0%
CAGTCAATAATATCTCCATC+AGG - chr2_3:36544982-36545001 None:intergenic 35.0%
! CCTTAATATGTCACTTGTTC+TGG - chr2_3:36545346-36545365 None:intergenic 35.0%
CCAGAACAAGTGACATATTA+AGG + chr2_3:36545343-36545362 Msa0289950:CDS 35.0%
!!! GCTAGCTCTGTTCAAAATTT+TGG + chr2_3:36545366-36545385 Msa0289950:CDS 35.0%
!! TCATCCATGATTTGTTATGC+CGG + chr2_3:36545617-36545636 Msa0289950:intron 35.0%
TGAGACAGACTGAATTCATT+AGG - chr2_3:36545934-36545953 None:intergenic 35.0%
!! TCTCAAATTTGGATTTTGGC+TGG + chr2_3:36545949-36545968 Msa0289950:CDS 35.0%
!!! TTTTTTCACGGGTTGAAAGT+CGG - chr2_3:36546108-36546127 None:intergenic 35.0%
CCCGTGAAAAAAACAATCAT+TGG + chr2_3:36546116-36546135 Msa0289950:intron 35.0%
ATTGGGAGCTAAGAAAAGTA+CGG - chr2_3:36546142-36546161 None:intergenic 35.0%
! AAGGGAAATGGACTTCAATT+GGG - chr2_3:36546159-36546178 None:intergenic 35.0%
! GAGTTTTGCTTCTAACAATC+CGG - chr2_3:36546187-36546206 None:intergenic 35.0%
TTTCTGCTCTTGCAACAATT+AGG + chr2_3:36546283-36546302 Msa0289950:intron 35.0%
TATTGTCACCGTACAAATCA+GGG - chr2_3:36546313-36546332 None:intergenic 35.0%
TTATTGTCACCGTACAAATC+AGG - chr2_3:36546314-36546333 None:intergenic 35.0%
ACACTAGACTCTTCACTTAT+TGG + chr2_3:36546333-36546352 Msa0289950:CDS 35.0%
AAGGAGATTATAACAACCAG+TGG - chr2_3:36546482-36546501 None:intergenic 35.0%
ATTAACAATGGTATAGCCCT+TGG + chr2_3:36546520-36546539 Msa0289950:CDS 35.0%
ATATCCCCACTTTCTAACTA+TGG + chr2_3:36546550-36546569 Msa0289950:CDS 35.0%
AGAACCATAGTTAGAAAGTG+GGG - chr2_3:36546557-36546576 None:intergenic 35.0%
GAAGAACCATAGTTAGAAAG+TGG - chr2_3:36546559-36546578 None:intergenic 35.0%
ATGATATAAGCATCTTGGTC+TGG + chr2_3:36546584-36546603 Msa0289950:CDS 35.0%
TGTAGTGTTAGTTAAGTGTG+GGG - chr2_3:36546671-36546690 None:intergenic 35.0%
!! GCTGTTATTTCGTAAATGCA+TGG + chr2_3:36546993-36547012 Msa0289950:intron 35.0%
TTTGGAAATGACCATGTTCT+AGG + chr2_3:36547198-36547217 Msa0289950:CDS 35.0%
! CATTTTCCTGATGAAGGTTT+TGG + chr2_3:36547342-36547361 Msa0289950:CDS 35.0%
!! TTGATGGTGACAACAAGTTA+AGG + chr2_3:36547397-36547416 Msa0289950:CDS 35.0%
! TGATGGTGACAACAAGTTAA+GGG + chr2_3:36547398-36547417 Msa0289950:CDS 35.0%
! GTGTAAGTACCAAGATCTTT+TGG - chr2_3:36547426-36547445 None:intergenic 35.0%
!!! AAGCTGTGGTTTTAAATTGC+AGG + chr2_3:36547558-36547577 Msa0289950:intron 35.0%
TAAATTGCAATCCACAACTG+CGG - chr2_3:36547607-36547626 None:intergenic 35.0%
GAAGCTCAAAATTCTCATTG+AGG + chr2_3:36547745-36547764 Msa0289950:three_prime_UTR 35.0%
TTGAGGTCTCCAAAAATTGT+TGG + chr2_3:36547762-36547781 Msa0289950:three_prime_UTR 35.0%
! CCTCTTTCTCCAACAATTTT+TGG - chr2_3:36547774-36547793 None:intergenic 35.0%
CCAAAAATTGTTGGAGAAAG+AGG + chr2_3:36547771-36547790 Msa0289950:three_prime_UTR 35.0%
TTGGGTTAAGGTTAAGCCTA+AGG + chr2_3:36544198-36544217 Msa0289950:five_prime_UTR 40.0%
! GCTTTAATGGAGGCCAATTT+TGG + chr2_3:36544444-36544463 Msa0289950:intron 40.0%
TGGATTATGCTTCTGCTTGT+TGG + chr2_3:36544464-36544483 Msa0289950:CDS 40.0%
!!! CTTCTGCTTGTTGGTTTTGT+TGG + chr2_3:36544473-36544492 Msa0289950:CDS 40.0%
!! TCTACCTTGATGGACTTAAC+AGG - chr2_3:36544621-36544640 None:intergenic 40.0%
!! GAACTTTAGTTCTTGGGAGT+TGG + chr2_3:36544746-36544765 Msa0289950:intron 40.0%
!! TCTTGTGATCTTTGAGGTCA+GGG - chr2_3:36545038-36545057 None:intergenic 40.0%
!! ATCTTGTGATCTTTGAGGTC+AGG - chr2_3:36545039-36545058 None:intergenic 40.0%
CCTGAATCTTGTGATCTTTG+AGG - chr2_3:36545044-36545063 None:intergenic 40.0%
CCTCAAAGATCACAAGATTC+AGG + chr2_3:36545041-36545060 Msa0289950:CDS 40.0%
TTCCCTTCTGGATGGAAATT+TGG - chr2_3:36545204-36545223 None:intergenic 40.0%
! CAAAAGTTTTCCCTTCTGGA+TGG - chr2_3:36545212-36545231 None:intergenic 40.0%
! CATCCAGAAGGGAAAACTTT+TGG + chr2_3:36545210-36545229 Msa0289950:CDS 40.0%
! TCTCCAAAAGTTTTCCCTTC+TGG - chr2_3:36545216-36545235 None:intergenic 40.0%
CAAAGCCTATAACTCAGCTT+TGG + chr2_3:36545274-36545293 Msa0289950:CDS 40.0%
TTCTGCCAAAGCTGAGTTAT+AGG - chr2_3:36545282-36545301 None:intergenic 40.0%
! ACTCAGCTTTGGCAGAAAAA+TGG + chr2_3:36545285-36545304 Msa0289950:CDS 40.0%
CTTGTTCTGGTCCTTCTTAT+AGG - chr2_3:36545333-36545352 None:intergenic 40.0%
ACTATAGCATGGAGATGCTT+TGG + chr2_3:36545755-36545774 Msa0289950:intron 40.0%
TTAGCATGCCATAGCTTATG+TGG + chr2_3:36545846-36545865 Msa0289950:intron 40.0%
AGGTGAATTCTATGGAGCAA+AGG + chr2_3:36545873-36545892 Msa0289950:CDS 40.0%
AAAGGCGACCATAAACGTTT+GGG + chr2_3:36545891-36545910 Msa0289950:CDS 40.0%
!! TTTTGGGTCCCAAACGTTTA+TGG - chr2_3:36545902-36545921 None:intergenic 40.0%
! ATCGATTCAACCTTAACTGG+AGG - chr2_3:36546076-36546095 None:intergenic 40.0%
CCAGTTAAGGTTGAATCGAT+CGG + chr2_3:36546076-36546095 Msa0289950:intron 40.0%
!! TTTTTCACGGGTTGAAAGTC+GGG - chr2_3:36546107-36546126 None:intergenic 40.0%
! CAAGGGAAATGGACTTCAAT+TGG - chr2_3:36546160-36546179 None:intergenic 40.0%
TCTAACAATCCGGTTCTCAA+GGG - chr2_3:36546177-36546196 None:intergenic 40.0%
TTCTAACAATCCGGTTCTCA+AGG - chr2_3:36546178-36546197 None:intergenic 40.0%
CAAAGTGGAGTGATTGCATT+GGG - chr2_3:36546369-36546388 None:intergenic 40.0%
ACAAAGTGGAGTGATTGCAT+TGG - chr2_3:36546370-36546389 None:intergenic 40.0%
TATAAGCATCTTGGTCTGGA+AGG + chr2_3:36546588-36546607 Msa0289950:CDS 40.0%
TAATGTGTAGGACCCAAAAG+AGG + chr2_3:36547158-36547177 Msa0289950:intron 40.0%
AATGTGTAGGACCCAAAAGA+GGG + chr2_3:36547159-36547178 Msa0289950:intron 40.0%
!! ATGACCATGTTCTAGGATAC+TGG + chr2_3:36547205-36547224 Msa0289950:CDS 40.0%
AGGTATGAGAATAAAGGAGC+TGG - chr2_3:36547231-36547250 None:intergenic 40.0%
! AGAGTGCCTCAATGATTGAA+TGG + chr2_3:36547256-36547275 Msa0289950:CDS 40.0%
GAGTGCCTCAATGATTGAAT+GGG + chr2_3:36547257-36547276 Msa0289950:CDS 40.0%
AGTGCCTCAATGATTGAATG+GGG + chr2_3:36547258-36547277 Msa0289950:CDS 40.0%
GTTGTGAACTCTGAATCTGA+TGG + chr2_3:36547288-36547307 Msa0289950:CDS 40.0%
ACAGCACACATCAACACAAA+TGG + chr2_3:36547311-36547330 Msa0289950:CDS 40.0%
CAGCACACATCAACACAAAT+GGG + chr2_3:36547312-36547331 Msa0289950:CDS 40.0%
ACATCAACACAAATGGGAAG+TGG + chr2_3:36547318-36547337 Msa0289950:CDS 40.0%
! AGTGGACATTTTCCTGATGA+AGG + chr2_3:36547336-36547355 Msa0289950:CDS 40.0%
!! TTAAGGGCACCAAAAGATCT+TGG + chr2_3:36547414-36547433 Msa0289950:CDS 40.0%
! AATGTCAAAACTGGTAGTGC+TGG + chr2_3:36547465-36547484 Msa0289950:CDS 40.0%
CTACTTCTATTATGGTGGTC+CGG + chr2_3:36547500-36547519 Msa0289950:CDS 40.0%
TACTTCTATTATGGTGGTCC+GGG + chr2_3:36547501-36547520 Msa0289950:CDS 40.0%
TAAATGCGGTTGCAATATCG+TGG + chr2_3:36547581-36547600 Msa0289950:intron 40.0%
! TGTAAGTTGAGCTACCACTT+AGG + chr2_3:36547706-36547725 Msa0289950:three_prime_UTR 40.0%
GAGCTACCACTTAGGTAATA+TGG + chr2_3:36547714-36547733 Msa0289950:three_prime_UTR 40.0%
! TTTTTGTCTGCTCCTATCTC+TGG + chr2_3:36547867-36547886 Msa0289950:three_prime_UTR 40.0%
TTGGTTCCTTTCACTTGTGC+TGG + chr2_3:36544492-36544511 Msa0289950:CDS 45.0%
!! ACCTTGATGGACTTAACAGG+CGG - chr2_3:36544618-36544637 None:intergenic 45.0%
TTGTGAAAGCAGAGTCCTGA+TGG + chr2_3:36544964-36544983 Msa0289950:intron 45.0%
ATGGGTTTGTGATGCAGATG+AGG + chr2_3:36545178-36545197 Msa0289950:intron 45.0%
AGGCCAAATTTCCATCCAGA+AGG + chr2_3:36545198-36545217 Msa0289950:CDS 45.0%
GGCCAAATTTCCATCCAGAA+GGG + chr2_3:36545199-36545218 Msa0289950:CDS 45.0%
!!! GGTTTTGCAGGTTTTGGTTG+AGG - chr2_3:36545414-36545433 None:intergenic 45.0%
! GGTAGAGGTTTTGCAGGTTT+TGG - chr2_3:36545420-36545439 None:intergenic 45.0%
! ATGTCAGGTAGAGGTTTTGC+AGG - chr2_3:36545426-36545445 None:intergenic 45.0%
TGAGTCAAGATGTCAGGTAG+AGG - chr2_3:36545435-36545454 None:intergenic 45.0%
!! TGGTGACTTGAACTTGCTGT+AGG - chr2_3:36545502-36545521 None:intergenic 45.0%
TGGACCGGCATAACAAATCA+TGG - chr2_3:36545624-36545643 None:intergenic 45.0%
AAAAAAGGACCAGACCTCTG+CGG - chr2_3:36545720-36545739 None:intergenic 45.0%
CATGCCATAGCTTATGTGGA+GGG + chr2_3:36545850-36545869 Msa0289950:CDS 45.0%
CAAAGGCGACCATAAACGTT+TGG + chr2_3:36545890-36545909 Msa0289950:CDS 45.0%
GCTGTTGAGATCTTGACCAA+AGG - chr2_3:36545977-36545996 None:intergenic 45.0%
! GATCTCAACAGCATTGAAGC+AGG + chr2_3:36545985-36546004 Msa0289950:CDS 45.0%
TGGTTAATGGCCTCCAGTTA+AGG + chr2_3:36546063-36546082 Msa0289950:intron 45.0%
! CCGATCGATTCAACCTTAAC+TGG - chr2_3:36546079-36546098 None:intergenic 45.0%
AGTCCATTTCCCTTGAGAAC+CGG + chr2_3:36546165-36546184 Msa0289950:intron 45.0%
AATCCGGTTCTCAAGGGAAA+TGG - chr2_3:36546171-36546190 None:intergenic 45.0%
! TAGGTCAGCCCTGATTTGTA+CGG + chr2_3:36546302-36546321 Msa0289950:intron 45.0%
AGTGATGCATATCAAGCCAC+TGG + chr2_3:36546463-36546482 Msa0289950:CDS 45.0%
TGGGGATATACTTGCTCCAA+GGG - chr2_3:36546539-36546558 None:intergenic 45.0%
AATTTGACATCGGAGTCGTC+TGG + chr2_3:36546950-36546969 Msa0289950:intron 45.0%
TGCATGGAATCTATGACGGT+AGG + chr2_3:36547009-36547028 Msa0289950:intron 45.0%
! TCCACCAGTTTCCCTCTTTT+GGG - chr2_3:36547173-36547192 None:intergenic 45.0%
! ATCCACCAGTTTCCCTCTTT+TGG - chr2_3:36547174-36547193 None:intergenic 45.0%
! GGAAACTGGTGGATGCAATT+TGG + chr2_3:36547180-36547199 Msa0289950:CDS 45.0%
! TCATTGAGGCACTCTCAGTT+AGG - chr2_3:36547251-36547270 None:intergenic 45.0%
ACCACCCCATTCAATCATTG+AGG - chr2_3:36547265-36547284 None:intergenic 45.0%
!! TCCTGATGAAGGTTTTGGCA+AGG + chr2_3:36547347-36547366 Msa0289950:CDS 45.0%
!! AAACTGGTAGTGCTGGTGAT+TGG + chr2_3:36547472-36547491 Msa0289950:CDS 45.0%
!! AACTGGTAGTGCTGGTGATT+GGG + chr2_3:36547473-36547492 Msa0289950:CDS 45.0%
! TGGGGCACCTACTTCTATTA+TGG + chr2_3:36547492-36547511 Msa0289950:CDS 45.0%
GGCACCTACTTCTATTATGG+TGG + chr2_3:36547495-36547514 Msa0289950:CDS 45.0%
CGGACCACCATAATAGAAGT+AGG - chr2_3:36547502-36547521 None:intergenic 45.0%
TTGTTGGAGAAAGAGGGTGA+TGG + chr2_3:36547778-36547797 Msa0289950:three_prime_UTR 45.0%
TGTTGGAGAAAGAGGGTGAT+GGG + chr2_3:36547779-36547798 Msa0289950:three_prime_UTR 45.0%
!! AAAAAAATATAAAAAAAACT+AGG + chr2_3:36547654-36547673 Msa0289950:three_prime_UTR 5.0%
!! AAAAAATATAAAAAAAACTA+GGG + chr2_3:36547655-36547674 Msa0289950:three_prime_UTR 5.0%
!!! ATATGTATATTTATTTATTT+TGG + chr2_3:36547953-36547972 Msa0289950:three_prime_UTR 5.0%
!! GTTTTGTTGGTGGGGTGCAT+AGG + chr2_3:36544162-36544181 Msa0289950:five_prime_UTR 50.0%
ACCGCCTGTTAAGTCCATCA+AGG + chr2_3:36544614-36544633 Msa0289950:CDS 50.0%
CAGGGTGATCAAAAGCTGGT+TGG - chr2_3:36545020-36545039 None:intergenic 50.0%
AGGTCAGGGTGATCAAAAGC+TGG - chr2_3:36545024-36545043 None:intergenic 50.0%
CCACTCTGAGTCAAGATGTC+AGG - chr2_3:36545441-36545460 None:intergenic 50.0%
CCTGACATCTTGACTCAGAG+TGG + chr2_3:36545438-36545457 Msa0289950:CDS 50.0%
CTTGACTCAGAGTGGTCATC+AGG + chr2_3:36545446-36545465 Msa0289950:CDS 50.0%
GCATGCCATAGCTTATGTGG+AGG + chr2_3:36545849-36545868 Msa0289950:intron 50.0%
ACCTCCCTCCACATAAGCTA+TGG - chr2_3:36545857-36545876 None:intergenic 50.0%
GTGGAGGGAGGTGAATTCTA+TGG + chr2_3:36545865-36545884 Msa0289950:CDS 50.0%
!!! TGGATTTTGGCTGGTGCCTT+TGG + chr2_3:36545958-36545977 Msa0289950:CDS 50.0%
! TCAACAGCATTGAAGCAGGC+TGG + chr2_3:36545989-36546008 Msa0289950:CDS 50.0%
GTGGGGATATACTTGCTCCA+AGG - chr2_3:36546540-36546559 None:intergenic 50.0%
AGGACCCAAAAGAGGGAAAC+TGG + chr2_3:36547166-36547185 Msa0289950:intron 50.0%
ACCCAAAAGAGGGAAACTGG+TGG + chr2_3:36547169-36547188 Msa0289950:CDS 50.0%
CTGGCCAGTATCCTAGAACA+TGG - chr2_3:36547212-36547231 None:intergenic 50.0%
GCCTCAATGATTGAATGGGG+TGG + chr2_3:36547261-36547280 Msa0289950:CDS 50.0%
GCCTTGCCAAAACCTTCATC+AGG - chr2_3:36547351-36547370 None:intergenic 50.0%
!! ACTGGTAGTGCTGGTGATTG+GGG + chr2_3:36547474-36547493 Msa0289950:CDS 50.0%
GCTTTCACAGCTCCAGAGAT+AGG - chr2_3:36547882-36547901 None:intergenic 50.0%
!! ATCTCTGGAGCTGTGAAAGC+TGG + chr2_3:36547882-36547901 Msa0289950:three_prime_UTR 50.0%
GCTCTGCCAGCACAAGTGAA+AGG - chr2_3:36544501-36544520 None:intergenic 55.0%
ATGACTCGTTCACACCGCAG+AGG + chr2_3:36545703-36545722 Msa0289950:intron 55.0%
GCCATAGCTTATGTGGAGGG+AGG + chr2_3:36545853-36545872 Msa0289950:CDS 55.0%
GGACAGTTAGGGTTTCGACC+CGG - chr2_3:36547522-36547541 None:intergenic 55.0%
CAATATCGTGGCCGCAGTTG+TGG + chr2_3:36547593-36547612 Msa0289950:intron 55.0%
! TCTGGAGCTGTGAAAGCTGG+TGG + chr2_3:36547885-36547904 Msa0289950:three_prime_UTR 55.0%
CTGGAGCTGTGAAAGCTGGT+GGG + chr2_3:36547886-36547905 Msa0289950:three_prime_UTR 55.0%
ATCAGCACCACCACCGGGAA+CGG + chr2_3:36544525-36544544 Msa0289950:CDS 60.0%
TCGTTCACACCGCAGAGGTC+TGG + chr2_3:36545708-36545727 Msa0289950:intron 60.0%
! CAGCATTGAAGCAGGCTGGC+AGG + chr2_3:36545993-36546012 Msa0289950:CDS 60.0%
GCCACCATCAGCACCACCAC+CGG + chr2_3:36544519-36544538 Msa0289950:CDS 65.0%
CCACCATCAGCACCACCACC+GGG + chr2_3:36544520-36544539 Msa0289950:CDS 65.0%
! GTTCCCGGTGGTGGTGCTGA+TGG - chr2_3:36544526-36544545 None:intergenic 65.0%
GCTCTCACCACCACCGTTCC+CGG - chr2_3:36544541-36544560 None:intergenic 65.0%
! GGGAACGGTGGTGGTGAGAG+CGG + chr2_3:36544540-36544559 Msa0289950:CDS 65.0%
! AACGGTGGTGGTGAGAGCGG+TGG + chr2_3:36544543-36544562 Msa0289950:CDS 65.0%
!! CCCGGTGGTGGTGCTGATGG+TGG - chr2_3:36544523-36544542 None:intergenic 70.0%
AGCACCACCACCGGGAACGG+TGG + chr2_3:36544528-36544547 Msa0289950:CDS 70.0%
ACCACCACCGGGAACGGTGG+TGG + chr2_3:36544531-36544550 Msa0289950:CDS 70.0%
ACCACCACCGTTCCCGGTGG+TGG - chr2_3:36544535-36544554 None:intergenic 70.0%
CTCACCACCACCGTTCCCGG+TGG - chr2_3:36544538-36544557 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 36544135 36548030 36544135 ID=Msa0289950;Name=Msa0289950
chr2_3 mRNA 36544135 36548030 36544135 ID=Msa0289950-mRNA-1;Parent=Msa0289950;Name=Msa0289950-mRNA-1;_AED=0.17;_eAED=0.17;_QI=90|1|1|1|0.75|0.55|9|416|429
chr2_3 exon 36544135 36544219 36544135 ID=Msa0289950-mRNA-1:exon:11950;Parent=Msa0289950-mRNA-1
chr2_3 exon 36544445 36544635 36544445 ID=Msa0289950-mRNA-1:exon:11951;Parent=Msa0289950-mRNA-1
chr2_3 exon 36544976 36545062 36544976 ID=Msa0289950-mRNA-1:exon:11952;Parent=Msa0289950-mRNA-1
chr2_3 exon 36545195 36545467 36545195 ID=Msa0289950-mRNA-1:exon:11953;Parent=Msa0289950-mRNA-1
chr2_3 exon 36545850 36546014 36545850 ID=Msa0289950-mRNA-1:exon:11954;Parent=Msa0289950-mRNA-1
chr2_3 exon 36546305 36546358 36546305 ID=Msa0289950-mRNA-1:exon:11955;Parent=Msa0289950-mRNA-1
chr2_3 exon 36546463 36546609 36546463 ID=Msa0289950-mRNA-1:exon:11956;Parent=Msa0289950-mRNA-1
chr2_3 exon 36547168 36547554 36547168 ID=Msa0289950-mRNA-1:exon:11957;Parent=Msa0289950-mRNA-1
chr2_3 exon 36547624 36548030 36547624 ID=Msa0289950-mRNA-1:exon:11958;Parent=Msa0289950-mRNA-1
chr2_3 five_prime_UTR 36544135 36544219 36544135 ID=Msa0289950-mRNA-1:five_prime_utr;Parent=Msa0289950-mRNA-1
chr2_3 five_prime_UTR 36544445 36544449 36544445 ID=Msa0289950-mRNA-1:five_prime_utr;Parent=Msa0289950-mRNA-1
chr2_3 CDS 36544450 36544635 36544450 ID=Msa0289950-mRNA-1:cds;Parent=Msa0289950-mRNA-1
chr2_3 CDS 36544976 36545062 36544976 ID=Msa0289950-mRNA-1:cds;Parent=Msa0289950-mRNA-1
chr2_3 CDS 36545195 36545467 36545195 ID=Msa0289950-mRNA-1:cds;Parent=Msa0289950-mRNA-1
chr2_3 CDS 36545850 36546014 36545850 ID=Msa0289950-mRNA-1:cds;Parent=Msa0289950-mRNA-1
chr2_3 CDS 36546305 36546358 36546305 ID=Msa0289950-mRNA-1:cds;Parent=Msa0289950-mRNA-1
chr2_3 CDS 36546463 36546609 36546463 ID=Msa0289950-mRNA-1:cds;Parent=Msa0289950-mRNA-1
chr2_3 CDS 36547168 36547545 36547168 ID=Msa0289950-mRNA-1:cds;Parent=Msa0289950-mRNA-1
chr2_3 three_prime_UTR 36547546 36547554 36547546 ID=Msa0289950-mRNA-1:three_prime_utr;Parent=Msa0289950-mRNA-1
chr2_3 three_prime_UTR 36547624 36548030 36547624 ID=Msa0289950-mRNA-1:three_prime_utr;Parent=Msa0289950-mRNA-1
Gene Sequence

>Msa0289950

ATGGAGGCCAATTTTGGATTATGCTTCTGCTTGTTGGTTTTGTTGGTTCCTTTCACTTGTGCTGGCAGAGCCACCATCAGCACCACCACCGGGAACGGTGGTGGTGAGAGCGGTGGTTCAATTCAGAAACTTGAGGTTAAGAAGCACTTGAAGAATTTGAACAGACCGCCTGTTAAGTCCATCAAGAGTCCTGATGGAGATATTATTGACTGTGTTCATGTTTCACACCAACCAGCTTTTGATCACCCTGACCTCAAAGATCACAAGATTCAGATGAGGCCAAATTTCCATCCAGAAGGGAAAACTTTTGGAGAGAGCAAAGTCTCTTCAACTTCAAATTCAAATTCAAATTCAAAGCCTATAACTCAGCTTTGGCAGAAAAATGGTATGTGTTCTGAAGGAACAATTCCTATAAGAAGGACCAGAACAAGTGACATATTAAGGGCTAGCTCTGTTCAAAATTTTGGAAAGAAGAAGCATAAAAGTACTCCTCAACCAAAACCTGCAAAACCTCTACCTGACATCTTGACTCAGAGTGGTCATCAGCATGCCATAGCTTATGTGGAGGGAGGTGAATTCTATGGAGCAAAGGCGACCATAAACGTTTGGGACCCAAAAATTCAACAACCTAATGAATTCAGTCTGTCTCAAATTTGGATTTTGGCTGGTGCCTTTGGTCAAGATCTCAACAGCATTGAAGCAGGCTGGCAGGTCAGCCCTGATTTGTACGGTGACAATAACACTAGACTCTTCACTTATTGGACAAGTGATGCATATCAAGCCACTGGTTGTTATAATCTCCTTTGTTCTGGCTTTATTCAAATTAACAATGGTATAGCCCTTGGAGCAAGTATATCCCCACTTTCTAACTATGGTTCTTCCCAATATGATATAAGCATCTTGGTCTGGAAGGACCCAAAAGAGGGAAACTGGTGGATGCAATTTGGAAATGACCATGTTCTAGGATACTGGCCAGCTCCTTTATTCTCATACCTAACTGAGAGTGCCTCAATGATTGAATGGGGTGGTGAAGTTGTGAACTCTGAATCTGATGGACAGCACACATCAACACAAATGGGAAGTGGACATTTTCCTGATGAAGGTTTTGGCAAGGCTAGTTATTTCAAAAATATTCAGGTTGTTGATGGTGACAACAAGTTAAGGGCACCAAAAGATCTTGGTACTTACACTGAAAAAGATAACTGTTATAATGTCAAAACTGGTAGTGCTGGTGATTGGGGCACCTACTTCTATTATGGTGGTCCGGGTCGAAACCCTAACTGTCCTTGA
Protein sequence

>Msa0289950

MEANFGLCFCLLVLLVPFTCAGRATISTTTGNGGGESGGSIQKLEVKKHLKNLNRPPVKSIKSPDGDIIDCVHVSHQPAFDHPDLKDHKIQMRPNFHPEGKTFGESKVSSTSNSNSNSKPITQLWQKNGMCSEGTIPIRRTRTSDILRASSVQNFGKKKHKSTPQPKPAKPLPDILTQSGHQHAIAYVEGGEFYGAKATINVWDPKIQQPNEFSLSQIWILAGAFGQDLNSIEAGWQVSPDLYGDNNTRLFTYWTSDAYQATGCYNLLCSGFIQINNGIALGASISPLSNYGSSQYDISILVWKDPKEGNWWMQFGNDHVLGYWPAPLFSYLTESASMIEWGGEVVNSESDGQHTSTQMGSGHFPDEGFGKASYFKNIQVVDGDNKLRAPKDLGTYTEKDNCYNVKTGSAGDWGTYFYYGGPGRNPNCP*