AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0327750

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0327750 MtrunA17_Chr2g0309251 97.658 427 9 1 1 427 1 426 0.0 865
Msa0327750 MtrunA17_Chr8g0380511 72.922 421 97 4 7 426 11 415 0.0 637
Msa0327750 MtrunA17_Chr7g0274951 54.953 424 181 6 12 426 17 439 9.29e-171 485
Msa0327750 MtrunA17_Chr1g0189961 53.103 435 183 7 9 427 22 451 6.61e-170 484
Msa0327750 MtrunA17_Chr3g0117271 54.074 405 164 7 25 427 112 496 1.18e-155 449
Msa0327750 MtrunA17_Chr8g0368161 54.663 386 154 5 43 427 83 448 1.02e-153 442
Msa0327750 MtrunA17_Chr4g0058771 55.385 390 153 7 40 427 111 481 9.08e-153 441
Msa0327750 MtrunA17_Chr5g0413131 53.627 386 152 5 46 427 101 463 1.07e-145 422
Msa0327750 MtrunA17_Chr2g0314451 48.454 388 174 8 41 427 43 405 9.79e-125 367
Msa0327750 MtrunA17_Chr2g0314461 48.964 386 171 8 43 427 46 406 1.20e-124 367
Msa0327750 MtrunA17_Chr8g0368241 46.632 386 185 5 43 427 49 414 5.23e-123 363
Msa0327750 MtrunA17_Chr2g0314471 48.187 386 174 8 43 427 46 406 9.00e-123 362
Msa0327750 MtrunA17_Chr8g0368201 48.148 324 139 6 121 427 9 320 1.90e-103 310
Msa0327750 MtrunA17_Chr3g0135381 41.902 389 212 5 45 424 51 434 8.40e-103 313
Msa0327750 MtrunA17_Chr6g0453411 41.755 376 201 9 62 427 8 375 7.51e-102 308
Msa0327750 MtrunA17_Chr3g0135371 39.898 391 221 7 45 427 51 435 6.95e-99 302
Msa0327750 MtrunA17_Chr8g0368291 39.578 427 212 12 12 427 14 405 1.64e-96 295
Msa0327750 MtrunA17_Chr2g0326371 49.835 303 130 8 130 427 75 360 4.60e-90 277
Msa0327750 MtrunA17_Chr8g0368341 40.872 367 180 8 63 427 4 335 2.10e-87 269
Msa0327750 MtrunA17_Chr3g0135401 39.067 343 191 6 94 424 11 347 1.62e-79 249
Msa0327750 MtrunA17_Chr8g0368181 58.031 193 80 1 235 427 2 193 6.87e-78 239
Msa0327750 MtrunA17_Chr1g0151871 38.961 308 167 4 121 427 40 327 6.66e-75 237
Msa0327750 MtrunA17_Chr8g0368221 47.638 254 107 6 50 302 30 258 5.75e-71 224
Msa0327750 MtrunA17_Chr8g0368361 51.282 195 92 3 235 427 2 195 5.25e-70 219
Msa0327750 MtrunA17_Chr3g0129891 50.971 206 93 3 223 426 180 379 5.90e-69 223
Msa0327750 MtrunA17_Chr3g0129891 45.312 128 53 4 43 163 79 196 7.05e-20 90.9
Msa0327750 MtrunA17_Chr8g0368331 41.877 277 129 4 180 427 13 286 1.03e-66 214
Msa0327750 MtrunA17_Chr8g0368281 46.632 193 102 1 235 426 2 194 1.22e-57 187
Msa0327750 MtrunA17_Chr3g0135431 34.078 358 151 9 36 319 35 381 2.55e-56 191
Msa0327750 MtrunA17_Chr3g0138811 32.527 372 206 15 59 423 53 386 1.41e-51 178
Msa0327750 MtrunA17_Chr3g0135441 36.626 243 131 5 188 427 10 232 3.73e-51 172
Msa0327750 MtrunA17_Chr8g0368351 41.739 230 102 7 11 236 47 248 1.82e-45 158
Msa0327750 MtrunA17_Chr8g0368261 57.480 127 52 2 302 427 4 129 4.27e-45 152
Msa0327750 MtrunA17_Chr2g0302691 30.541 370 211 13 60 421 37 368 7.89e-43 154
Msa0327750 MtrunA17_Chr8g0339871 38.211 246 137 12 190 426 2 241 1.78e-41 147
Msa0327750 MtrunA17_Chr8g0368231 53.175 126 57 2 303 427 8 132 5.98e-41 142
Msa0327750 MtrunA17_Chr8g0391201 36.066 244 141 11 191 426 3 239 1.85e-38 139
Msa0327750 MtrunA17_Chr8g0368381 47.788 113 59 0 235 347 2 114 6.41e-38 134
Msa0327750 MtrunA17_Chr5g0445181 31.636 275 152 10 90 364 61 299 1.21e-32 125
Msa0327750 MtrunA17_Chr8g0368171 32.766 235 115 6 5 236 10 204 5.05e-28 110
Msa0327750 MtrunA17_Chr3g0135451 42.424 132 66 2 35 156 28 159 9.20e-27 106
Msa0327750 MtrunA17_Chr8g0353931 30.476 210 114 6 33 236 20 203 5.10e-24 99.0
Msa0327750 MtrunA17_Chr2g0302651 30.942 223 122 11 213 426 1 200 4.52e-22 93.6
Msa0327750 MtrunA17_Chr8g0353941 35.638 188 111 7 240 423 1 182 9.99e-22 92.0
Msa0327750 MtrunA17_Chr5g0426971 28.390 236 142 7 188 421 46 256 4.61e-21 92.0
Msa0327750 MtrunA17_Chr5g0434771 35.849 159 94 5 248 404 12 164 1.32e-20 89.0
Msa0327750 MtrunA17_Chr8g0353961 35.556 135 65 4 31 154 12 135 3.92e-20 86.7
Msa0327750 MtrunA17_Chr8g0353971 32.164 171 108 4 255 421 4 170 1.38e-19 85.9
Msa0327750 MtrunA17_Chr8g0368321 41.026 117 53 4 41 156 41 142 8.60e-17 77.8
Msa0327750 MtrunA17_Chr8g0368271 33.333 153 87 4 9 156 11 153 1.08e-16 78.2
Msa0327750 MtrunA17_Chr5g0443801 36.697 109 67 2 190 298 21 127 1.84e-15 74.3
Msa0327750 MtrunA17_Chr3g0135411 61.224 49 19 0 45 93 64 112 6.40e-14 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0327750 AT3G13510.1 70.726 427 109 4 1 427 9 419 0.0 630
Msa0327750 AT1G55360.1 71.028 428 106 5 1 427 12 422 0.0 624
Msa0327750 AT5G56530.2 69.668 422 111 4 5 426 15 419 0.0 614
Msa0327750 AT5G56530.1 69.668 422 111 4 5 426 15 419 0.0 614
Msa0327750 AT2G44210.1 68.608 395 118 5 35 427 25 415 0.0 576
Msa0327750 AT2G44210.2 63.765 425 118 6 35 427 25 445 0.0 561
Msa0327750 AT5G18460.1 58.462 390 155 5 40 426 44 429 8.99e-165 470
Msa0327750 AT2G44220.1 51.938 387 171 4 42 427 31 403 3.17e-156 447
Msa0327750 AT1G10750.1 55.527 389 151 5 41 427 99 467 2.87e-155 447
Msa0327750 AT5G50150.1 54.242 389 157 5 41 427 51 420 2.82e-153 441
Msa0327750 AT1G23340.2 50.117 429 192 5 1 427 1 409 7.22e-152 437
Msa0327750 AT1G23340.1 50.117 429 192 5 1 427 1 409 7.22e-152 437
Msa0327750 AT1G70550.1 53.213 389 160 5 41 427 97 465 1.05e-149 433
Msa0327750 AT2G44240.1 48.122 426 192 7 3 427 5 402 6.65e-141 409
Msa0327750 AT2G17750.1 45.455 418 200 7 9 426 6 395 6.83e-134 390
Msa0327750 AT2G19360.1 46.404 431 199 7 14 426 9 425 3.08e-131 385
Msa0327750 AT2G44250.1 44.131 426 208 9 5 426 9 408 1.90e-121 359
Msa0327750 AT3G48230.1 43.896 385 187 11 50 427 11 373 4.17e-110 329
Msa0327750 AT5G19170.1 40.722 388 199 10 40 421 3 365 5.72e-94 288
Msa0327750 AT1G23340.3 48.684 304 134 6 1 302 1 284 1.54e-93 284
Msa0327750 AT5G25950.1 40.943 403 207 9 44 427 42 432 3.06e-93 288
Msa0327750 AT2G44250.2 45.833 312 159 7 119 426 20 325 2.33e-91 279
Msa0327750 AT2G20170.1 39.276 387 206 13 42 421 32 396 2.83e-85 266
Msa0327750 AT2G20170.2 39.276 387 206 13 42 421 32 396 2.83e-85 266
Msa0327750 AT5G25960.1 39.433 388 178 8 44 427 23 357 2.82e-81 254
Msa0327750 AT2G20170.3 36.176 387 201 13 42 421 32 379 8.41e-74 236
Msa0327750 AT4G23370.1 36.031 383 213 14 50 421 40 401 5.36e-70 227
Msa0327750 AT4G23355.1 35.878 393 210 14 42 421 35 398 6.89e-69 224
Msa0327750 AT4G23350.1 33.333 387 230 10 42 421 35 400 6.61e-68 221
Msa0327750 AT4G23390.1 35.696 381 212 14 50 421 41 397 4.70e-66 216
Msa0327750 AT4G23365.1 33.588 393 219 14 42 421 35 398 1.66e-65 215
Msa0327750 AT4G23360.1 34.015 391 221 12 42 421 35 399 8.66e-65 213
Msa0327750 AT4G23380.1 32.308 390 227 11 42 421 36 398 2.35e-62 207
Msa0327750 AT1G70550.2 49.767 215 86 6 41 253 97 291 9.87e-61 199
Msa0327750 AT2G35250.1 35.635 362 182 12 67 423 24 339 1.87e-55 187
Msa0327750 AT1G10750.2 43.162 234 105 7 46 274 105 315 3.37e-53 181
Msa0327750 AT1G10190.1 32.750 400 224 17 35 421 27 394 6.67e-50 174
Msa0327750 AT4G15050.1 33.002 403 219 18 35 421 27 394 8.56e-48 168
Msa0327750 AT4G15053.1 31.282 390 232 15 42 421 38 401 3.39e-46 164
Msa0327750 AT5G46810.1 30.435 368 211 13 70 421 11 349 3.10e-44 158
Msa0327750 AT2G27320.1 34.505 313 157 11 69 378 30 297 3.46e-42 151
Msa0327750 AT4G17505.1 29.189 370 193 10 58 424 18 321 7.63e-41 148
Msa0327750 AT5G46820.1 27.676 383 221 13 55 421 5 347 1.26e-40 148
Msa0327750 AT4G10220.1 29.337 392 228 16 42 421 44 398 3.66e-39 145
Msa0327750 AT4G23373.1 32.308 260 133 8 13 272 7 223 7.42e-39 142
Msa0327750 AT5G05030.1 30.000 380 214 17 54 423 22 359 9.07e-39 143
Msa0327750 AT2G24950.1 29.108 426 249 19 8 421 25 409 1.33e-38 144
Msa0327750 AT5G25410.1 27.273 374 228 12 54 421 28 363 1.82e-37 140
Msa0327750 AT5G60380.1 28.457 376 219 15 57 421 31 367 2.45e-35 134
Msa0327750 AT5G46200.1 27.249 389 232 13 70 421 31 405 3.80e-35 134
Msa0327750 AT4G10210.1 27.990 418 251 16 1 404 3 384 8.89e-33 128
Msa0327750 AT4G17860.1 28.533 368 202 16 70 421 29 351 1.65e-31 124
Msa0327750 AT4G23080.1 27.982 436 256 19 10 423 7 406 7.58e-31 122
Msa0327750 AT2G38255.1 33.632 223 105 7 70 290 14 195 8.56e-30 115
Msa0327750 AT5G11660.1 30.368 326 188 14 112 423 4 304 1.36e-28 114
Msa0327750 AT2G03930.2 42.105 114 62 3 168 279 31 142 7.46e-23 95.1
Msa0327750 AT5G25415.1 27.545 334 202 13 106 423 23 332 9.51e-23 99.0
Msa0327750 AT5G36680.1 27.213 305 181 11 129 421 52 327 1.21e-19 89.7
Msa0327750 AT5G36770.1 27.213 305 181 11 129 421 52 327 1.21e-19 89.7
Msa0327750 AT5G37520.1 32.328 232 116 13 197 422 2 198 6.67e-19 85.1
Msa0327750 AT5G04937.1 30.000 210 120 7 219 421 9 198 3.51e-18 83.2

Find 140 sgRNAs with CRISPR-Local

Find 270 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTAGCTCTGTTCAAAATTT+TGG 0.058994 2_4:+39697506 Msa0327750:CDS
ATCCACCAGTTTCCCTCTTT+TGG 0.121092 2_4:-39699316 None:intergenic
AATTCAGTCTGTCTCAAATT+TGG 0.126548 2_4:+39698081 Msa0327750:CDS
TTCCCTTCTGGATGGAAATT+TGG 0.152045 2_4:-39697347 None:intergenic
ATATGTATATTTATTTATTT+TGG 0.152989 2_4:+39700101 Msa0327750:three_prime_UTR
ACACTAGACTCTTCACTTAT+TGG 0.158217 2_4:+39698484 Msa0327750:CDS
GCTTTAATGGAGGCCAATTT+TGG 0.214424 2_4:+39696539 Msa0327750:intron
TCTGTCTCAAATTTGGATTT+TGG 0.224441 2_4:+39698088 Msa0327750:CDS
CCAGAACAAGTGACATATTA+AGG 0.229209 2_4:+39697483 Msa0327750:CDS
CATAGGATAAGCTTCAAAAT+TGG 0.235823 2_4:+39696274 Msa0327750:five_prime_UTR
TTCTGCCAAAGCTGAGTTAT+AGG 0.242996 2_4:-39697419 None:intergenic
TGTTATAATCTCCTTTGTTC+TGG 0.263241 2_4:+39698641 Msa0327750:CDS
TGCATTTGGAGAAGCTTTAA+TGG 0.266793 2_4:+39696526 Msa0327750:intron
CCTTAATATGTCACTTGTTC+TGG 0.271266 2_4:-39697483 None:intergenic
CTTGTTCTGGTCCTTCTTAT+AGG 0.284686 2_4:-39697470 None:intergenic
TTTGGAAATGACCATGTTCT+AGG 0.286569 2_4:+39699343 Msa0327750:CDS
CCAAGATGCTTATATCATAT+TGG 0.295235 2_4:-39698733 None:intergenic
CCTGAATCTTGTGATCTTTG+AGG 0.299817 2_4:-39697165 None:intergenic
GCAGAAGCATAATCCAAAAT+TGG 0.311137 2_4:-39696552 None:intergenic
GTGTAAGTACCAAGATCTTT+TGG 0.311380 2_4:-39699568 None:intergenic
TGGATTATGCTTCTGCTTGT+TGG 0.325487 2_4:+39696559 Msa0327750:CDS
TGGGGCACCTACTTCTATTA+TGG 0.331136 2_4:+39699637 Msa0327750:CDS
GGAAACTGGTGGATGCAATT+TGG 0.341439 2_4:+39699325 Msa0327750:CDS
ATAAAAGATCAAGGACAGTT+AGG 0.341879 2_4:-39699676 None:intergenic
TGAGACAGACTGAATTCATT+AGG 0.345968 2_4:-39698074 None:intergenic
ATATCCCCACTTTCTAACTA+TGG 0.346949 2_4:+39698704 Msa0327750:CDS
AAAACTTATCTCTACCTTGA+TGG 0.347925 2_4:-39696723 None:intergenic
GAGCTATCACTTAGGTAATA+TGG 0.349074 2_4:+39699860 Msa0327750:three_prime_UTR
AACTGGTAGTGCTGGTGATT+GGG 0.351854 2_4:+39699618 Msa0327750:CDS
GCTCTGCCAGCACAAGTAAA+AGG 0.352627 2_4:-39696593 None:intergenic
CCTCAAAGATCACAAGATTC+AGG 0.363603 2_4:+39697165 Msa0327750:CDS
ATCTTGTGATCTTTGAGGTC+AGG 0.364868 2_4:-39697160 None:intergenic
CAAGATGCTTATATCATATT+GGG 0.373261 2_4:-39698732 None:intergenic
ACTCTCACCACCACCGTTCC+CGG 0.380605 2_4:-39696633 None:intergenic
AACTGTTATAATGTCAAAAC+TGG 0.390356 2_4:+39699601 Msa0327750:CDS
TTATTGTCTCCATACAAATC+AGG 0.392038 2_4:-39698462 None:intergenic
TGAACAGAATATTCTCTAGT+AGG 0.392533 2_4:+39700066 Msa0327750:three_prime_UTR
GCTTTCACAGCTCCAGAGAT+AGG 0.400467 2_4:-39700027 None:intergenic
CTACTTCTATTATGGTGGTC+CGG 0.402420 2_4:+39699645 Msa0327750:CDS
TAAGCTTCAAAATTGGGTTA+AGG 0.406474 2_4:+39696281 Msa0327750:five_prime_UTR
GTGGAGGGAGGTGAATTCTA+TGG 0.407436 2_4:+39698008 Msa0327750:CDS
ATAGGATAAGCTTCAAAATT+GGG 0.416322 2_4:+39696275 Msa0327750:five_prime_UTR
CAAAGGCGACCATAAACGTT+TGG 0.418700 2_4:+39698033 Msa0327750:CDS
TCTACCTTGATGGACTTAAC+AGG 0.423789 2_4:-39696713 None:intergenic
TTGATGGTGACAACAAGTTA+AGG 0.430415 2_4:+39699542 Msa0327750:CDS
GCCACCATCAGCACCACCAC+CGG 0.431095 2_4:+39696614 Msa0327750:CDS
TAGGTCAGCCCTGATTTGTA+TGG 0.432543 2_4:+39698453 Msa0327750:intron
ATGACCATGTTCTAGGATAC+TGG 0.437458 2_4:+39699350 Msa0327750:CDS
CAAAGCCTATAACTCAGCTT+TGG 0.444736 2_4:+39697414 Msa0327750:CDS
ACCACCACCGTTCCCGGTGG+TGG 0.447471 2_4:-39696627 None:intergenic
TAAAAGATCAAGGACAGTTA+GGG 0.448907 2_4:-39699675 None:intergenic
ACAGCACACATCAACACAAA+TGG 0.449226 2_4:+39699456 Msa0327750:CDS
CCAATATGATATAAGCATCT+TGG 0.452731 2_4:+39698733 Msa0327750:CDS
TTAAGGGCTCCAAAAGATCT+TGG 0.459260 2_4:+39699559 Msa0327750:CDS
AAACTGGTAGTGCTGGTGAT+TGG 0.461992 2_4:+39699617 Msa0327750:CDS
AGGACCCAAAAGAGGGAAAC+TGG 0.463817 2_4:+39699311 Msa0327750:intron
TCAGTTAGGTATGAGAATAA+AGG 0.467064 2_4:-39699379 None:intergenic
CTTGACTCAGAGTGGTCATC+AGG 0.468890 2_4:+39697586 Msa0327750:CDS
TGATGGTGACAACAAGTTAA+GGG 0.475300 2_4:+39699543 Msa0327750:CDS
TGTTGGAGAAAGAGGGTGAT+GGG 0.475386 2_4:+39699925 Msa0327750:three_prime_UTR
ATCTCTGGAGCTGTGAAAGC+TGG 0.479478 2_4:+39700030 Msa0327750:three_prime_UTR
AAAGGCGACCATAAACGTTT+GGG 0.483661 2_4:+39698034 Msa0327750:CDS
TCATTGAGGCACTCTCAGTT+AGG 0.484694 2_4:-39699393 None:intergenic
TACTTCTATTATGGTGGTCC+GGG 0.485729 2_4:+39699646 Msa0327750:CDS
ATTAACAATGGTATAGCCCT+TGG 0.488395 2_4:+39698674 Msa0327750:CDS
GCCTTGCCAAAACCTTCATC+AGG 0.488665 2_4:-39699493 None:intergenic
ATGATATAAGCATCTTGGTC+TGG 0.490781 2_4:+39698738 Msa0327750:CDS
AGAGTGCCTCAATGATTGAA+TGG 0.494264 2_4:+39699401 Msa0327750:CDS
TGGATTGCAATTTAAAACTA+CGG 0.494301 2_4:+39699758 Msa0327750:intron
AGTGATGCATATCAAGCAAC+TGG 0.496993 2_4:+39698617 Msa0327750:CDS
AAGGAACAATTCCTATAAGA+AGG 0.498065 2_4:+39697459 Msa0327750:CDS
ACCACCACCGGGAACGGTGG+TGG 0.505459 2_4:+39696626 Msa0327750:CDS
CAGCATTGAAGCAGGCTGGC+AGG 0.505596 2_4:+39698136 Msa0327750:CDS
GAACAGAATATTCTCTAGTA+GGG 0.506018 2_4:+39700067 Msa0327750:three_prime_UTR
TTGGGTTAAGGTTAAGCCTA+AGG 0.507890 2_4:+39696293 Msa0327750:five_prime_UTR
CCACTCTGAGTCAAGATGTC+AGG 0.518935 2_4:-39697578 None:intergenic
CAGCACACATCAACACAAAT+GGG 0.520852 2_4:+39699457 Msa0327750:CDS
CAGAACAAGTGACATATTAA+GGG 0.521105 2_4:+39697484 Msa0327750:CDS
GAGTGCCTCAATGATTGAAT+GGG 0.527263 2_4:+39699402 Msa0327750:CDS
GTTCCCGGTGGTGGTGCTGA+TGG 0.531155 2_4:-39696618 None:intergenic
TTAGCATGCCATAGCTTATG+TGG 0.531230 2_4:+39697989 Msa0327750:intron
AATGTCAAAACTGGTAGTGC+TGG 0.534590 2_4:+39699610 Msa0327750:CDS
GGCACCTACTTCTATTATGG+TGG 0.539245 2_4:+39699640 Msa0327750:CDS
GATCTCAACAGCATTGAAGC+AGG 0.539985 2_4:+39698128 Msa0327750:CDS
GAAGAACCATAGTTAGAAAG+TGG 0.541399 2_4:-39698710 None:intergenic
TTGTGAAAGCAGAGTCCTGA+TGG 0.541959 2_4:+39697088 Msa0327750:intron
CCACCATCAGCACCACCACC+GGG 0.545767 2_4:+39696615 Msa0327750:CDS
AGGTCAGGGTGATCAAAAGC+TGG 0.547634 2_4:-39697145 None:intergenic
TCTGGAGCTGTGAAAGCTGG+TGG 0.550324 2_4:+39700033 Msa0327750:three_prime_UTR
GGGAACGGTGGTGGTGAGAG+TGG 0.552058 2_4:+39696635 Msa0327750:CDS
ACCTCCCTCCACATAAGCTA+TGG 0.557000 2_4:-39697997 None:intergenic
CCCGGTGGTGGTGCTGATGG+TGG 0.557572 2_4:-39696615 None:intergenic
TAATGTGTAGGACCCAAAAG+AGG 0.564000 2_4:+39699303 Msa0327750:intron
GGCTTTATTCAAATTAACAA+TGG 0.564459 2_4:+39698662 Msa0327750:CDS
TGGGGATATACTTGCTCCAA+GGG 0.564591 2_4:-39698690 None:intergenic
ACATCAACACAAATGGGAAG+TGG 0.565243 2_4:+39699463 Msa0327750:CDS
CAGTCAATAATATCTCCATC+AGG 0.565977 2_4:-39697103 None:intergenic
AGCTTTAACATAAAAGATCA+AGG 0.566363 2_4:-39699685 None:intergenic
TCTTGTGATCTTTGAGGTCA+GGG 0.570285 2_4:-39697159 None:intergenic
AGGTATGAGAATAAAGGAGC+TGG 0.573128 2_4:-39699373 None:intergenic
GAAGCTCAAAATTCTCATTG+AGG 0.576114 2_4:+39699891 Msa0327750:three_prime_UTR
CTGGAGCTGTGAAAGCTGGT+GGG 0.576766 2_4:+39700034 Msa0327750:three_prime_UTR
ACCACCCCATTCAATCATTG+AGG 0.578345 2_4:-39699407 None:intergenic
TATTGTCTCCATACAAATCA+GGG 0.581583 2_4:-39698461 None:intergenic
GTGGGGATATACTTGCTCCA+AGG 0.581673 2_4:-39698691 None:intergenic
TCAACAGCATTGAAGCAGGC+TGG 0.583391 2_4:+39698132 Msa0327750:CDS
AGGCCAAATTTCCATCCAGA+AGG 0.584529 2_4:+39697344 Msa0327750:CDS
CGGACCACCATAATAGAAGT+AGG 0.584855 2_4:-39699644 None:intergenic
TATAAGCATCTTGGTCTGGA+AGG 0.589005 2_4:+39698742 Msa0327750:CDS
AACGGTGGTGGTGAGAGTGG+TGG 0.589657 2_4:+39696638 Msa0327750:CDS
TGTAAGTTGAGCTATCACTT+AGG 0.592148 2_4:+39699852 Msa0327750:three_prime_UTR
TTGTTGGAGAAAGAGGGTGA+TGG 0.593904 2_4:+39699924 Msa0327750:three_prime_UTR
TGGTTCAATTCAGAAACTTG+AGG 0.594105 2_4:+39696658 Msa0327750:CDS
AAGAACCATAGTTAGAAAGT+GGG 0.595857 2_4:-39698709 None:intergenic
ACTGGTAGTGCTGGTGATTG+GGG 0.595966 2_4:+39699619 Msa0327750:CDS
TTTGAATAAAGCCAGAACAA+AGG 0.597232 2_4:-39698652 None:intergenic
TGAGTCAAGATGTCAGGTAG+AGG 0.600193 2_4:-39697572 None:intergenic
ATCAGCACCACCACCGGGAA+CGG 0.601551 2_4:+39696620 Msa0327750:CDS
CAGGGTGATCAAAAGCTGGT+TGG 0.603523 2_4:-39697141 None:intergenic
CTGGCCAGTATCCTAGAACA+TGG 0.608996 2_4:-39699354 None:intergenic
AGCACCACCACCGGGAACGG+TGG 0.613079 2_4:+39696623 Msa0327750:CDS
AGGTGAATTCTATGGAGCAA+AGG 0.618255 2_4:+39698016 Msa0327750:CDS
AATGTGTAGGACCCAAAAGA+GGG 0.621507 2_4:+39699304 Msa0327750:intron
ACCACCTGTTAAGTCCATCA+AGG 0.622540 2_4:+39696709 Msa0327750:CDS
AACAGAATATTCTCTAGTAG+GGG 0.643070 2_4:+39700068 Msa0327750:three_prime_UTR
GGCCAAATTTCCATCCAGAA+GGG 0.643729 2_4:+39697345 Msa0327750:CDS
CATGCCATAGCTTATGTGGA+GGG 0.644068 2_4:+39697993 Msa0327750:CDS
GCATGCCATAGCTTATGTGG+AGG 0.646750 2_4:+39697992 Msa0327750:intron
AGAACCATAGTTAGAAAGTG+GGG 0.647282 2_4:-39698708 None:intergenic
ATTTGGAGAAGCTTTAATGG+AGG 0.650311 2_4:+39696529 Msa0327750:intron
GCTGTTGAGATCTTGACCAA+AGG 0.656530 2_4:-39698117 None:intergenic
GCCATAGCTTATGTGGAGGG+AGG 0.658878 2_4:+39697996 Msa0327750:CDS
GGACAGTTAGGGTTTCGACC+CGG 0.661025 2_4:-39699664 None:intergenic
GTAGTGAACTCTGAGTCTGA+TGG 0.662522 2_4:+39699433 Msa0327750:CDS
GCCTCAATGATTGAATGGGG+TGG 0.671790 2_4:+39699406 Msa0327750:CDS
ACCTTGATGGACTTAACAGG+TGG 0.674625 2_4:-39696710 None:intergenic
CTCACCACCACCGTTCCCGG+TGG 0.688072 2_4:-39696630 None:intergenic
CCTGACATCTTGACTCAGAG+TGG 0.701149 2_4:+39697578 Msa0327750:CDS
ACCCAAAAGAGGGAAACTGG+TGG 0.707549 2_4:+39699314 Msa0327750:CDS
AGTGCCTCAATGATTGAATG+GGG 0.753510 2_4:+39699403 Msa0327750:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAAAATATAGAACTTTTTA+TGG + chr2_4:39696410-39696429 Msa0327750:intron 10.0%
!!! TTGTAAAAAATTAAACTTTT+TGG + chr2_4:39696826-39696845 Msa0327750:intron 10.0%
!!! TGTAAAAAATTAAACTTTTT+GGG + chr2_4:39696827-39696846 Msa0327750:intron 10.0%
!!! TGATTTAATTTTTAGATTAT+AGG + chr2_4:39697016-39697035 Msa0327750:intron 10.0%
!! ATAATAATTCAAACAATTAT+GGG + chr2_4:39697306-39697325 Msa0327750:intron 10.0%
!!! CAAAAAATAACATTTTAAAA+AGG - chr2_4:39698774-39698793 None:intergenic 10.0%
!!! AAAATGTTATTTTTTGTTAT+TGG + chr2_4:39698778-39698797 Msa0327750:intron 10.0%
!! TATAGCATATACAATTAAAT+GGG - chr2_4:39696385-39696404 None:intergenic 15.0%
!! TTATAGCATATACAATTAAA+TGG - chr2_4:39696386-39696405 None:intergenic 15.0%
!! CATAATAATTCAAACAATTA+TGG + chr2_4:39697305-39697324 Msa0327750:intron 15.0%
!! AACATATACTATCATAAAAA+AGG - chr2_4:39697878-39697897 None:intergenic 15.0%
!! TAAAACAAATACATTTAATC+AGG - chr2_4:39697940-39697959 None:intergenic 15.0%
!! ATTGATACATATTAAATTCA+TGG - chr2_4:39699251-39699270 None:intergenic 15.0%
!! TGAATAAAAAAATAATGTGT+AGG + chr2_4:39699291-39699310 Msa0327750:intron 15.0%
!!! TAGTTATTTCAAAAATATTC+AGG + chr2_4:39699516-39699535 Msa0327750:CDS 15.0%
!!! TAGAACTTTTTATGGTTTTT+TGG + chr2_4:39696418-39696437 Msa0327750:intron 20.0%
!!! ATAACGGTTTTATTTCTAAT+TGG + chr2_4:39696800-39696819 Msa0327750:intron 20.0%
!!! TAACGGTTTTATTTCTAATT+GGG + chr2_4:39696801-39696820 Msa0327750:intron 20.0%
!!! TTAGTAGTGTATTTTTGTAT+TGG + chr2_4:39696957-39696976 Msa0327750:intron 20.0%
!! AAATGAACTATAGTTATGAA+TGG - chr2_4:39697043-39697062 None:intergenic 20.0%
!! TCAATAATTGACGAATAAAT+TGG - chr2_4:39697803-39697822 None:intergenic 20.0%
!!! TCCTTTTGATTTTATTTTCT+TGG + chr2_4:39697961-39697980 Msa0327750:intron 20.0%
!! TCCAAGAAAATAAAATCAAA+AGG - chr2_4:39697965-39697984 None:intergenic 20.0%
!! TTTAAAAGCTAATTTGACAT+CGG + chr2_4:39699086-39699105 Msa0327750:intron 20.0%
! ATAGGATAAGCTTCAAAATT+GGG + chr2_4:39696275-39696294 Msa0327750:five_prime_UTR 25.0%
! TGTATGTAATATTCTACCTT+AGG - chr2_4:39696312-39696331 None:intergenic 25.0%
! CAAAGACTAATGAAGTTAAA+GGG - chr2_4:39696447-39696466 None:intergenic 25.0%
! ACAAAGACTAATGAAGTTAA+AGG - chr2_4:39696448-39696467 None:intergenic 25.0%
! CTTTATGAGAATTGTCAAAT+GGG - chr2_4:39696489-39696508 None:intergenic 25.0%
! ACTTTATGAGAATTGTCAAA+TGG - chr2_4:39696490-39696509 None:intergenic 25.0%
!!! TTAAACTTTTTGGGTTTGAA+AGG + chr2_4:39696836-39696855 Msa0327750:intron 25.0%
! ATTGTAGTGAAAAAGACTAA+AGG - chr2_4:39696890-39696909 None:intergenic 25.0%
!!! AGTCTTTTTCACTACAATAA+TGG + chr2_4:39696892-39696911 Msa0327750:intron 25.0%
! ATTGCAAGAACAAAAACAAT+AGG - chr2_4:39696925-39696944 None:intergenic 25.0%
!! TTTCTTTCATTTGTGTCATT+TGG + chr2_4:39697262-39697281 Msa0327750:intron 25.0%
! TAGTATATGTTACTATAGCA+TGG + chr2_4:39697887-39697906 Msa0327750:intron 25.0%
! TTTAATGAAAGAACAGAAAG+TGG - chr2_4:39698536-39698555 None:intergenic 25.0%
!!! GGCTTTATTCAAATTAACAA+TGG + chr2_4:39698662-39698681 Msa0327750:CDS 25.0%
! CAAGATGCTTATATCATATT+GGG - chr2_4:39698735-39698754 None:intergenic 25.0%
!! GGCATATTTTCTTTCTATAA+TGG - chr2_4:39698804-39698823 None:intergenic 25.0%
! AAATAACAGCTTAGAATCAT+TGG - chr2_4:39699129-39699148 None:intergenic 25.0%
! AAATTTGAATTCGTACACAT+GGG + chr2_4:39699178-39699197 Msa0327750:intron 25.0%
!!! AAAAATATTCAGGTTGTTGA+TGG + chr2_4:39699526-39699545 Msa0327750:CDS 25.0%
! AACTGTTATAATGTCAAAAC+TGG + chr2_4:39699601-39699620 Msa0327750:CDS 25.0%
!! AGCTTTAACATAAAAGATCA+AGG - chr2_4:39699688-39699707 None:intergenic 25.0%
!! TTTTAAATTGCAGGTAAATG+CGG + chr2_4:39699712-39699731 Msa0327750:intron 25.0%
!! TGGATTGCAATTTAAAACTA+CGG + chr2_4:39699758-39699777 Msa0327750:intron 25.0%
!! AGCTTTTCATTAATCAAACA+TGG - chr2_4:39700129-39700148 None:intergenic 25.0%
!!! GTTGTTGTTGTTTGTTTTGT+TGG + chr2_4:39696244-39696263 Msa0327750:five_prime_UTR 30.0%
!!! TTGTTGTTTGTTTTGTTGGT+GGG + chr2_4:39696248-39696267 Msa0327750:five_prime_UTR 30.0%
CATAGGATAAGCTTCAAAAT+TGG + chr2_4:39696274-39696293 Msa0327750:five_prime_UTR 30.0%
TAAGCTTCAAAATTGGGTTA+AGG + chr2_4:39696281-39696300 Msa0327750:five_prime_UTR 30.0%
AAAACTTATCTCTACCTTGA+TGG - chr2_4:39696726-39696745 None:intergenic 30.0%
TTATGCAACCAATGAAAAAG+TGG - chr2_4:39696784-39696803 None:intergenic 30.0%
!! CTTTTTCATTGGTTGCATAA+CGG + chr2_4:39696784-39696803 Msa0327750:intron 30.0%
!! TTGAAAGGAACTTTAGTTCT+TGG + chr2_4:39696851-39696870 Msa0327750:intron 30.0%
!! TGAAAGGAACTTTAGTTCTT+GGG + chr2_4:39696852-39696871 Msa0327750:intron 30.0%
!!! ATGCAAGTGTACTTGTTTTA+TGG + chr2_4:39697063-39697082 Msa0327750:intron 30.0%
AAAAATGGTATGTGTTCTGA+AGG + chr2_4:39697440-39697459 Msa0327750:CDS 30.0%
AAGGAACAATTCCTATAAGA+AGG + chr2_4:39697459-39697478 Msa0327750:CDS 30.0%
CAGAACAAGTGACATATTAA+GGG + chr2_4:39697484-39697503 Msa0327750:CDS 30.0%
TAGGTGCATTATAAGTAACA+TGG - chr2_4:39697623-39697642 None:intergenic 30.0%
GGTTATTGAAATTTATGCAC+CGG - chr2_4:39697782-39697801 None:intergenic 30.0%
!! CATTAGGTTGCTGAATTTTT+GGG - chr2_4:39698061-39698080 None:intergenic 30.0%
!! TCATTAGGTTGCTGAATTTT+TGG - chr2_4:39698062-39698081 None:intergenic 30.0%
AATTCAGTCTGTCTCAAATT+TGG + chr2_4:39698081-39698100 Msa0327750:CDS 30.0%
! TCTGTCTCAAATTTGGATTT+TGG + chr2_4:39698088-39698107 Msa0327750:CDS 30.0%
TGTTTGACTCAATTGGTTAA+TGG + chr2_4:39698191-39698210 Msa0327750:intron 30.0%
TATTGTCTCCATACAAATCA+GGG - chr2_4:39698464-39698483 None:intergenic 30.0%
TTATTGTCTCCATACAAATC+AGG - chr2_4:39698465-39698484 None:intergenic 30.0%
TGTTATAATCTCCTTTGTTC+TGG + chr2_4:39698641-39698660 Msa0327750:CDS 30.0%
TTTGAATAAAGCCAGAACAA+AGG - chr2_4:39698655-39698674 None:intergenic 30.0%
AAGAACCATAGTTAGAAAGT+GGG - chr2_4:39698712-39698731 None:intergenic 30.0%
CCAAGATGCTTATATCATAT+TGG - chr2_4:39698736-39698755 None:intergenic 30.0%
CCAATATGATATAAGCATCT+TGG + chr2_4:39698733-39698752 Msa0327750:CDS 30.0%
TTGTAGTGTTAGTTAAGTGT+GGG - chr2_4:39698826-39698845 None:intergenic 30.0%
ATTGTAGTGTTAGTTAAGTG+TGG - chr2_4:39698827-39698846 None:intergenic 30.0%
ATGCGTGTATAATGTATGTA+TGG + chr2_4:39698941-39698960 Msa0327750:intron 30.0%
! AGTATTGGATGAAAATCAGA+CGG + chr2_4:39699054-39699073 Msa0327750:intron 30.0%
TAAATGCATGGAATCTATGA+CGG + chr2_4:39699151-39699170 Msa0327750:intron 30.0%
GAAATTTGAATTCGTACACA+TGG + chr2_4:39699177-39699196 Msa0327750:intron 30.0%
TCAGTTAGGTATGAGAATAA+AGG - chr2_4:39699382-39699401 None:intergenic 30.0%
TAAAAGATCAAGGACAGTTA+GGG - chr2_4:39699678-39699697 None:intergenic 30.0%
ATAAAAGATCAAGGACAGTT+AGG - chr2_4:39699679-39699698 None:intergenic 30.0%
! GATCTTTTATGTTAAAGCTG+TGG + chr2_4:39699689-39699708 Msa0327750:intron 30.0%
CAAAAATTGTTGGAGAAAGA+GGG + chr2_4:39699918-39699937 Msa0327750:three_prime_UTR 30.0%
TGAACAGAATATTCTCTAGT+AGG + chr2_4:39700066-39700085 Msa0327750:three_prime_UTR 30.0%
GAACAGAATATTCTCTAGTA+GGG + chr2_4:39700067-39700086 Msa0327750:three_prime_UTR 30.0%
AACAGAATATTCTCTAGTAG+GGG + chr2_4:39700068-39700087 Msa0327750:three_prime_UTR 30.0%
!!! GTTGTTGTTTGTTTTGTTGG+TGG + chr2_4:39696247-39696266 Msa0327750:five_prime_UTR 35.0%
!!! TGTTGTTTGTTTTGTTGGTG+GGG + chr2_4:39696249-39696268 Msa0327750:five_prime_UTR 35.0%
! CATGCTTTTGCATTTGCATT+TGG + chr2_4:39696512-39696531 Msa0327750:intron 35.0%
! TGCATTTGGAGAAGCTTTAA+TGG + chr2_4:39696526-39696545 Msa0327750:intron 35.0%
! ATTTGGAGAAGCTTTAATGG+AGG + chr2_4:39696529-39696548 Msa0327750:intron 35.0%
! GCAGAAGCATAATCCAAAAT+TGG - chr2_4:39696555-39696574 None:intergenic 35.0%
TGGTTCAATTCAGAAACTTG+AGG + chr2_4:39696658-39696677 Msa0327750:CDS 35.0%
!!! TTACTCTGCCACTTTTTCAT+TGG + chr2_4:39696773-39696792 Msa0327750:intron 35.0%
!! AACTTTAGTTCTTGGGAGTT+GGG + chr2_4:39696859-39696878 Msa0327750:intron 35.0%
CAGTCAATAATATCTCCATC+AGG - chr2_4:39697106-39697125 None:intergenic 35.0%
! TCTTCAAAAGTTTTCCCTTC+TGG - chr2_4:39697362-39697381 None:intergenic 35.0%
! CCTTAATATGTCACTTGTTC+TGG - chr2_4:39697486-39697505 None:intergenic 35.0%
CCAGAACAAGTGACATATTA+AGG + chr2_4:39697483-39697502 Msa0327750:CDS 35.0%
!!! GCTAGCTCTGTTCAAAATTT+TGG + chr2_4:39697506-39697525 Msa0327750:CDS 35.0%
!! TAGTGACTTAAACTTGCTGT+AGG - chr2_4:39697642-39697661 None:intergenic 35.0%
!! TCATCCATGATTTGTTATGC+CGG + chr2_4:39697760-39697779 Msa0327750:intron 35.0%
TGAGACAGACTGAATTCATT+AGG - chr2_4:39698077-39698096 None:intergenic 35.0%
!! TCTCAAATTTGGATTTTGGC+TGG + chr2_4:39698092-39698111 Msa0327750:CDS 35.0%
CATCTTCTGTTTGACTCAAT+TGG + chr2_4:39698184-39698203 Msa0327750:intron 35.0%
! TTTTGGCCATGGTTGAAATT+CGG - chr2_4:39698249-39698268 None:intergenic 35.0%
! AGTGTGGTCAATGATTGTTT+TGG - chr2_4:39698266-39698285 None:intergenic 35.0%
ATTGGGAGATAAGAAAAGTG+TGG - chr2_4:39698282-39698301 None:intergenic 35.0%
! AAGGGAAATGGACTTCAATT+GGG - chr2_4:39698299-39698318 None:intergenic 35.0%
TTTCTGCTCTTGCAACAATT+AGG + chr2_4:39698434-39698453 Msa0327750:intron 35.0%
ACACTAGACTCTTCACTTAT+TGG + chr2_4:39698484-39698503 Msa0327750:CDS 35.0%
! AAAGTGGAGTGATTTGCATT+GGG - chr2_4:39698520-39698539 None:intergenic 35.0%
ATTAACAATGGTATAGCCCT+TGG + chr2_4:39698674-39698693 Msa0327750:CDS 35.0%
ATATCCCCACTTTCTAACTA+TGG + chr2_4:39698704-39698723 Msa0327750:CDS 35.0%
AGAACCATAGTTAGAAAGTG+GGG - chr2_4:39698711-39698730 None:intergenic 35.0%
GAAGAACCATAGTTAGAAAG+TGG - chr2_4:39698713-39698732 None:intergenic 35.0%
ATGATATAAGCATCTTGGTC+TGG + chr2_4:39698738-39698757 Msa0327750:CDS 35.0%
TGTAGTGTTAGTTAAGTGTG+GGG - chr2_4:39698825-39698844 None:intergenic 35.0%
! TCAGTCTCTTTTTGTATGTG+TGG + chr2_4:39698972-39698991 Msa0327750:intron 35.0%
! CAGTCTCTTTTTGTATGTGT+GGG + chr2_4:39698973-39698992 Msa0327750:intron 35.0%
! AGTCAACAGCTTAGAAGTAT+TGG + chr2_4:39699039-39699058 Msa0327750:intron 35.0%
!! GCTGTTATTTCGTAAATGCA+TGG + chr2_4:39699139-39699158 Msa0327750:intron 35.0%
TTTGGAAATGACCATGTTCT+AGG + chr2_4:39699343-39699362 Msa0327750:CDS 35.0%
! CATTTTCCTGATGAAGGTTT+TGG + chr2_4:39699487-39699506 Msa0327750:CDS 35.0%
!! TTGATGGTGACAACAAGTTA+AGG + chr2_4:39699542-39699561 Msa0327750:CDS 35.0%
! TGATGGTGACAACAAGTTAA+GGG + chr2_4:39699543-39699562 Msa0327750:CDS 35.0%
! GTGTAAGTACCAAGATCTTT+TGG - chr2_4:39699571-39699590 None:intergenic 35.0%
!!! AAGCTGTGGTTTTAAATTGC+AGG + chr2_4:39699703-39699722 Msa0327750:intron 35.0%
TAAATTGCAATCCACAACTG+CGG - chr2_4:39699752-39699771 None:intergenic 35.0%
! TGTAAGTTGAGCTATCACTT+AGG + chr2_4:39699852-39699871 Msa0327750:three_prime_UTR 35.0%
GAGCTATCACTTAGGTAATA+TGG + chr2_4:39699860-39699879 Msa0327750:three_prime_UTR 35.0%
GAAGCTCAAAATTCTCATTG+AGG + chr2_4:39699891-39699910 Msa0327750:three_prime_UTR 35.0%
TTGAGGTCTCCAAAAATTGT+TGG + chr2_4:39699908-39699927 Msa0327750:three_prime_UTR 35.0%
! CCTCTTTCTCCAACAATTTT+TGG - chr2_4:39699920-39699939 None:intergenic 35.0%
CCAAAAATTGTTGGAGAAAG+AGG + chr2_4:39699917-39699936 Msa0327750:three_prime_UTR 35.0%
TTGGGTTAAGGTTAAGCCTA+AGG + chr2_4:39696293-39696312 Msa0327750:five_prime_UTR 40.0%
! GCTTTAATGGAGGCCAATTT+TGG + chr2_4:39696539-39696558 Msa0327750:intron 40.0%
TGGATTATGCTTCTGCTTGT+TGG + chr2_4:39696559-39696578 Msa0327750:CDS 40.0%
!!! CTTCTGCTTGTTGGTTTTGT+TGG + chr2_4:39696568-39696587 Msa0327750:CDS 40.0%
! TTGGTTCCTTTTACTTGTGC+TGG + chr2_4:39696587-39696606 Msa0327750:CDS 40.0%
!! TCTACCTTGATGGACTTAAC+AGG - chr2_4:39696716-39696735 None:intergenic 40.0%
!! GAACTTTAGTTCTTGGGAGT+TGG + chr2_4:39696858-39696877 Msa0327750:intron 40.0%
!! TCTTGTGATCTTTGAGGTCA+GGG - chr2_4:39697162-39697181 None:intergenic 40.0%
!! ATCTTGTGATCTTTGAGGTC+AGG - chr2_4:39697163-39697182 None:intergenic 40.0%
CCTGAATCTTGTGATCTTTG+AGG - chr2_4:39697168-39697187 None:intergenic 40.0%
CCTCAAAGATCACAAGATTC+AGG + chr2_4:39697165-39697184 Msa0327750:CDS 40.0%
TTCCCTTCTGGATGGAAATT+TGG - chr2_4:39697350-39697369 None:intergenic 40.0%
! CAAAAGTTTTCCCTTCTGGA+TGG - chr2_4:39697358-39697377 None:intergenic 40.0%
CAAAGCCTATAACTCAGCTT+TGG + chr2_4:39697414-39697433 Msa0327750:CDS 40.0%
TTCTGCCAAAGCTGAGTTAT+AGG - chr2_4:39697422-39697441 None:intergenic 40.0%
! ACTCAGCTTTGGCAGAAAAA+TGG + chr2_4:39697425-39697444 Msa0327750:CDS 40.0%
CTTGTTCTGGTCCTTCTTAT+AGG - chr2_4:39697473-39697492 None:intergenic 40.0%
ACTATAGCATGGAGATGCTT+TGG + chr2_4:39697898-39697917 Msa0327750:intron 40.0%
TTAGCATGCCATAGCTTATG+TGG + chr2_4:39697989-39698008 Msa0327750:intron 40.0%
AGGTGAATTCTATGGAGCAA+AGG + chr2_4:39698016-39698035 Msa0327750:CDS 40.0%
AAAGGCGACCATAAACGTTT+GGG + chr2_4:39698034-39698053 Msa0327750:CDS 40.0%
!! TTTTGGGTCCCAAACGTTTA+TGG - chr2_4:39698045-39698064 None:intergenic 40.0%
AGTCAAACAGAAGATGACAG+TGG - chr2_4:39698180-39698199 None:intergenic 40.0%
AAGTAGCCGAATTTCAACCA+TGG + chr2_4:39698240-39698259 Msa0327750:intron 40.0%
!!! GTCAATGATTGTTTTGGCCA+TGG - chr2_4:39698260-39698279 None:intergenic 40.0%
! CAAGGGAAATGGACTTCAAT+TGG - chr2_4:39698300-39698319 None:intergenic 40.0%
AATCCAGTTCTCAAGGGAAA+TGG - chr2_4:39698311-39698330 None:intergenic 40.0%
TCTGACAATCCAGTTCTCAA+GGG - chr2_4:39698317-39698336 None:intergenic 40.0%
TTCTGACAATCCAGTTCTCA+AGG - chr2_4:39698318-39698337 None:intergenic 40.0%
! GAAAGTGGAGTGATTTGCAT+TGG - chr2_4:39698521-39698540 None:intergenic 40.0%
AGTGATGCATATCAAGCAAC+TGG + chr2_4:39698617-39698636 Msa0327750:CDS 40.0%
TATAAGCATCTTGGTCTGGA+AGG + chr2_4:39698742-39698761 Msa0327750:CDS 40.0%
TAATGTGTAGGACCCAAAAG+AGG + chr2_4:39699303-39699322 Msa0327750:intron 40.0%
AATGTGTAGGACCCAAAAGA+GGG + chr2_4:39699304-39699323 Msa0327750:intron 40.0%
!! ATGACCATGTTCTAGGATAC+TGG + chr2_4:39699350-39699369 Msa0327750:CDS 40.0%
AGGTATGAGAATAAAGGAGC+TGG - chr2_4:39699376-39699395 None:intergenic 40.0%
! AGAGTGCCTCAATGATTGAA+TGG + chr2_4:39699401-39699420 Msa0327750:CDS 40.0%
GAGTGCCTCAATGATTGAAT+GGG + chr2_4:39699402-39699421 Msa0327750:CDS 40.0%
AGTGCCTCAATGATTGAATG+GGG + chr2_4:39699403-39699422 Msa0327750:CDS 40.0%
ACAGCACACATCAACACAAA+TGG + chr2_4:39699456-39699475 Msa0327750:CDS 40.0%
CAGCACACATCAACACAAAT+GGG + chr2_4:39699457-39699476 Msa0327750:CDS 40.0%
ACATCAACACAAATGGGAAG+TGG + chr2_4:39699463-39699482 Msa0327750:CDS 40.0%
! AGTGGACATTTTCCTGATGA+AGG + chr2_4:39699481-39699500 Msa0327750:CDS 40.0%
TTAAGGGCTCCAAAAGATCT+TGG + chr2_4:39699559-39699578 Msa0327750:CDS 40.0%
! AATGTCAAAACTGGTAGTGC+TGG + chr2_4:39699610-39699629 Msa0327750:CDS 40.0%
CTACTTCTATTATGGTGGTC+CGG + chr2_4:39699645-39699664 Msa0327750:CDS 40.0%
TACTTCTATTATGGTGGTCC+GGG + chr2_4:39699646-39699665 Msa0327750:CDS 40.0%
TAAATGCGGTTGCAATATCG+TGG + chr2_4:39699726-39699745 Msa0327750:intron 40.0%
! TTTTTGTCTGCTCCTATCTC+TGG + chr2_4:39700015-39700034 Msa0327750:three_prime_UTR 40.0%
ACCACCTGTTAAGTCCATCA+AGG + chr2_4:39696709-39696728 Msa0327750:CDS 45.0%
!! ACCTTGATGGACTTAACAGG+TGG - chr2_4:39696713-39696732 None:intergenic 45.0%
TTGTGAAAGCAGAGTCCTGA+TGG + chr2_4:39697088-39697107 Msa0327750:intron 45.0%
ATGGGTTTGTGATGCAGATG+AGG + chr2_4:39697324-39697343 Msa0327750:intron 45.0%
AGGCCAAATTTCCATCCAGA+AGG + chr2_4:39697344-39697363 Msa0327750:CDS 45.0%
GGCCAAATTTCCATCCAGAA+GGG + chr2_4:39697345-39697364 Msa0327750:CDS 45.0%
!!! GGTTTTGCAGGTTTTGGTTG+AGG - chr2_4:39697554-39697573 None:intergenic 45.0%
! GGTAGAGGTTTTGCAGGTTT+TGG - chr2_4:39697560-39697579 None:intergenic 45.0%
! ATGTCAGGTAGAGGTTTTGC+AGG - chr2_4:39697566-39697585 None:intergenic 45.0%
TGAGTCAAGATGTCAGGTAG+AGG - chr2_4:39697575-39697594 None:intergenic 45.0%
! TGCACCGGCATAACAAATCA+TGG - chr2_4:39697767-39697786 None:intergenic 45.0%
AAAAAAGGACCAGACCTCTG+TGG - chr2_4:39697863-39697882 None:intergenic 45.0%
CATGCCATAGCTTATGTGGA+GGG + chr2_4:39697993-39698012 Msa0327750:CDS 45.0%
CAAAGGCGACCATAAACGTT+TGG + chr2_4:39698033-39698052 Msa0327750:CDS 45.0%
GCTGTTGAGATCTTGACCAA+AGG - chr2_4:39698120-39698139 None:intergenic 45.0%
! GATCTCAACAGCATTGAAGC+AGG + chr2_4:39698128-39698147 Msa0327750:CDS 45.0%
TGGTTAATGGCCTCCAGTTA+AGG + chr2_4:39698204-39698223 Msa0327750:intron 45.0%
!! ATCGATTCGACCTTAACTGG+AGG - chr2_4:39698217-39698236 None:intergenic 45.0%
AGTCCATTTCCCTTGAGAAC+TGG + chr2_4:39698305-39698324 Msa0327750:intron 45.0%
! TAGGTCAGCCCTGATTTGTA+TGG + chr2_4:39698453-39698472 Msa0327750:intron 45.0%
TGGGGATATACTTGCTCCAA+GGG - chr2_4:39698693-39698712 None:intergenic 45.0%
AATTTGACATCGGAGTCGTC+TGG + chr2_4:39699096-39699115 Msa0327750:intron 45.0%
! TCCACCAGTTTCCCTCTTTT+GGG - chr2_4:39699318-39699337 None:intergenic 45.0%
! ATCCACCAGTTTCCCTCTTT+TGG - chr2_4:39699319-39699338 None:intergenic 45.0%
! GGAAACTGGTGGATGCAATT+TGG + chr2_4:39699325-39699344 Msa0327750:CDS 45.0%
! TCATTGAGGCACTCTCAGTT+AGG - chr2_4:39699396-39699415 None:intergenic 45.0%
ACCACCCCATTCAATCATTG+AGG - chr2_4:39699410-39699429 None:intergenic 45.0%
GTAGTGAACTCTGAGTCTGA+TGG + chr2_4:39699433-39699452 Msa0327750:CDS 45.0%
!! TCCTGATGAAGGTTTTGGCA+AGG + chr2_4:39699492-39699511 Msa0327750:CDS 45.0%
!! AAACTGGTAGTGCTGGTGAT+TGG + chr2_4:39699617-39699636 Msa0327750:CDS 45.0%
!! AACTGGTAGTGCTGGTGATT+GGG + chr2_4:39699618-39699637 Msa0327750:CDS 45.0%
! TGGGGCACCTACTTCTATTA+TGG + chr2_4:39699637-39699656 Msa0327750:CDS 45.0%
GGCACCTACTTCTATTATGG+TGG + chr2_4:39699640-39699659 Msa0327750:CDS 45.0%
CGGACCACCATAATAGAAGT+AGG - chr2_4:39699647-39699666 None:intergenic 45.0%
TTGTTGGAGAAAGAGGGTGA+TGG + chr2_4:39699924-39699943 Msa0327750:three_prime_UTR 45.0%
TGTTGGAGAAAGAGGGTGAT+GGG + chr2_4:39699925-39699944 Msa0327750:three_prime_UTR 45.0%
!!! AAAAAATAACATTTTAAAAA+GGG - chr2_4:39698773-39698792 None:intergenic 5.0%
!! AAAAAAATATAAAAAAAACT+AGG + chr2_4:39699800-39699819 Msa0327750:three_prime_UTR 5.0%
!! AAAAAATATAAAAAAAACTA+GGG + chr2_4:39699801-39699820 Msa0327750:three_prime_UTR 5.0%
!!! ATATGTATATTTATTTATTT+TGG + chr2_4:39700101-39700120 Msa0327750:three_prime_UTR 5.0%
!! GTTTTGTTGGTGGGGTGCAT+AGG + chr2_4:39696257-39696276 Msa0327750:five_prime_UTR 50.0%
GCTCTGCCAGCACAAGTAAA+AGG - chr2_4:39696596-39696615 None:intergenic 50.0%
CAGGGTGATCAAAAGCTGGT+TGG - chr2_4:39697144-39697163 None:intergenic 50.0%
AGGTCAGGGTGATCAAAAGC+TGG - chr2_4:39697148-39697167 None:intergenic 50.0%
CCACTCTGAGTCAAGATGTC+AGG - chr2_4:39697581-39697600 None:intergenic 50.0%
CCTGACATCTTGACTCAGAG+TGG + chr2_4:39697578-39697597 Msa0327750:CDS 50.0%
CTTGACTCAGAGTGGTCATC+AGG + chr2_4:39697586-39697605 Msa0327750:CDS 50.0%
!! ATGACTTGTTCACGCCACAG+AGG + chr2_4:39697846-39697865 Msa0327750:intron 50.0%
GCATGCCATAGCTTATGTGG+AGG + chr2_4:39697992-39698011 Msa0327750:intron 50.0%
ACCTCCCTCCACATAAGCTA+TGG - chr2_4:39698000-39698019 None:intergenic 50.0%
GTGGAGGGAGGTGAATTCTA+TGG + chr2_4:39698008-39698027 Msa0327750:CDS 50.0%
!!! TGGATTTTGGCTGGTGCCTT+TGG + chr2_4:39698101-39698120 Msa0327750:CDS 50.0%
! TCAACAGCATTGAAGCAGGC+TGG + chr2_4:39698132-39698151 Msa0327750:CDS 50.0%
!! GCGATCGATTCGACCTTAAC+TGG - chr2_4:39698220-39698239 None:intergenic 50.0%
GTGGGGATATACTTGCTCCA+AGG - chr2_4:39698694-39698713 None:intergenic 50.0%
TGCATGGAATCTATGACGGC+AGG + chr2_4:39699155-39699174 Msa0327750:intron 50.0%
AGGACCCAAAAGAGGGAAAC+TGG + chr2_4:39699311-39699330 Msa0327750:intron 50.0%
ACCCAAAAGAGGGAAACTGG+TGG + chr2_4:39699314-39699333 Msa0327750:CDS 50.0%
CTGGCCAGTATCCTAGAACA+TGG - chr2_4:39699357-39699376 None:intergenic 50.0%
GCCTCAATGATTGAATGGGG+TGG + chr2_4:39699406-39699425 Msa0327750:CDS 50.0%
GCCTTGCCAAAACCTTCATC+AGG - chr2_4:39699496-39699515 None:intergenic 50.0%
!! ACTGGTAGTGCTGGTGATTG+GGG + chr2_4:39699619-39699638 Msa0327750:CDS 50.0%
GCTTTCACAGCTCCAGAGAT+AGG - chr2_4:39700030-39700049 None:intergenic 50.0%
!! ATCTCTGGAGCTGTGAAAGC+TGG + chr2_4:39700030-39700049 Msa0327750:three_prime_UTR 50.0%
TTGTTCACGCCACAGAGGTC+TGG + chr2_4:39697851-39697870 Msa0327750:intron 55.0%
GCCATAGCTTATGTGGAGGG+AGG + chr2_4:39697996-39698015 Msa0327750:CDS 55.0%
GGACAGTTAGGGTTTCGACC+CGG - chr2_4:39699667-39699686 None:intergenic 55.0%
CAATATCGTGGCCGCAGTTG+TGG + chr2_4:39699738-39699757 Msa0327750:intron 55.0%
! TCTGGAGCTGTGAAAGCTGG+TGG + chr2_4:39700033-39700052 Msa0327750:three_prime_UTR 55.0%
CTGGAGCTGTGAAAGCTGGT+GGG + chr2_4:39700034-39700053 Msa0327750:three_prime_UTR 55.0%
ATCAGCACCACCACCGGGAA+CGG + chr2_4:39696620-39696639 Msa0327750:CDS 60.0%
ACTCTCACCACCACCGTTCC+CGG - chr2_4:39696636-39696655 None:intergenic 60.0%
! AACGGTGGTGGTGAGAGTGG+TGG + chr2_4:39696638-39696657 Msa0327750:CDS 60.0%
! CAGCATTGAAGCAGGCTGGC+AGG + chr2_4:39698136-39698155 Msa0327750:CDS 60.0%
GCCACCATCAGCACCACCAC+CGG + chr2_4:39696614-39696633 Msa0327750:CDS 65.0%
CCACCATCAGCACCACCACC+GGG + chr2_4:39696615-39696634 Msa0327750:CDS 65.0%
! GTTCCCGGTGGTGGTGCTGA+TGG - chr2_4:39696621-39696640 None:intergenic 65.0%
! GGGAACGGTGGTGGTGAGAG+TGG + chr2_4:39696635-39696654 Msa0327750:CDS 65.0%
!! CCCGGTGGTGGTGCTGATGG+TGG - chr2_4:39696618-39696637 None:intergenic 70.0%
AGCACCACCACCGGGAACGG+TGG + chr2_4:39696623-39696642 Msa0327750:CDS 70.0%
ACCACCACCGGGAACGGTGG+TGG + chr2_4:39696626-39696645 Msa0327750:CDS 70.0%
ACCACCACCGTTCCCGGTGG+TGG - chr2_4:39696630-39696649 None:intergenic 70.0%
CTCACCACCACCGTTCCCGG+TGG - chr2_4:39696633-39696652 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 39696230 39700178 39696230 ID=Msa0327750;Name=Msa0327750
chr2_4 mRNA 39696230 39700178 39696230 ID=Msa0327750-mRNA-1;Parent=Msa0327750;Name=Msa0327750-mRNA-1;_AED=0.16;_eAED=0.16;_QI=90|1|1|1|0.75|0.55|9|419|427
chr2_4 exon 39696230 39696314 39696230 ID=Msa0327750-mRNA-1:exon:11983;Parent=Msa0327750-mRNA-1
chr2_4 exon 39696540 39696730 39696540 ID=Msa0327750-mRNA-1:exon:11984;Parent=Msa0327750-mRNA-1
chr2_4 exon 39697100 39697186 39697100 ID=Msa0327750-mRNA-1:exon:11985;Parent=Msa0327750-mRNA-1
chr2_4 exon 39697341 39697607 39697341 ID=Msa0327750-mRNA-1:exon:11986;Parent=Msa0327750-mRNA-1
chr2_4 exon 39697993 39698157 39697993 ID=Msa0327750-mRNA-1:exon:11987;Parent=Msa0327750-mRNA-1
chr2_4 exon 39698456 39698509 39698456 ID=Msa0327750-mRNA-1:exon:11988;Parent=Msa0327750-mRNA-1
chr2_4 exon 39698617 39698763 39698617 ID=Msa0327750-mRNA-1:exon:11989;Parent=Msa0327750-mRNA-1
chr2_4 exon 39699313 39699699 39699313 ID=Msa0327750-mRNA-1:exon:11990;Parent=Msa0327750-mRNA-1
chr2_4 exon 39699769 39700178 39699769 ID=Msa0327750-mRNA-1:exon:11991;Parent=Msa0327750-mRNA-1
chr2_4 five_prime_UTR 39696230 39696314 39696230 ID=Msa0327750-mRNA-1:five_prime_utr;Parent=Msa0327750-mRNA-1
chr2_4 five_prime_UTR 39696540 39696544 39696540 ID=Msa0327750-mRNA-1:five_prime_utr;Parent=Msa0327750-mRNA-1
chr2_4 CDS 39696545 39696730 39696545 ID=Msa0327750-mRNA-1:cds;Parent=Msa0327750-mRNA-1
chr2_4 CDS 39697100 39697186 39697100 ID=Msa0327750-mRNA-1:cds;Parent=Msa0327750-mRNA-1
chr2_4 CDS 39697341 39697607 39697341 ID=Msa0327750-mRNA-1:cds;Parent=Msa0327750-mRNA-1
chr2_4 CDS 39697993 39698157 39697993 ID=Msa0327750-mRNA-1:cds;Parent=Msa0327750-mRNA-1
chr2_4 CDS 39698456 39698509 39698456 ID=Msa0327750-mRNA-1:cds;Parent=Msa0327750-mRNA-1
chr2_4 CDS 39698617 39698763 39698617 ID=Msa0327750-mRNA-1:cds;Parent=Msa0327750-mRNA-1
chr2_4 CDS 39699313 39699690 39699313 ID=Msa0327750-mRNA-1:cds;Parent=Msa0327750-mRNA-1
chr2_4 three_prime_UTR 39699691 39699699 39699691 ID=Msa0327750-mRNA-1:three_prime_utr;Parent=Msa0327750-mRNA-1
chr2_4 three_prime_UTR 39699769 39700178 39699769 ID=Msa0327750-mRNA-1:three_prime_utr;Parent=Msa0327750-mRNA-1
Gene Sequence

>Msa0327750

ATGGAGGCCAATTTTGGATTATGCTTCTGCTTGTTGGTTTTGTTGGTTCCTTTTACTTGTGCTGGCAGAGCCACCATCAGCACCACCACCGGGAACGGTGGTGGTGAGAGTGGTGGTTCAATTCAGAAACTTGAGGTTAAGAAGCACTTGAAGAATTTGAACAGACCACCTGTTAAGTCCATCAAGAGTCCTGATGGAGATATTATTGACTGTGTTCATGTTTCACACCAACCAGCTTTTGATCACCCTGACCTCAAAGATCACAAGATTCAGATGAGGCCAAATTTCCATCCAGAAGGGAAAACTTTTGAAGAGAGCAAAGTCTCTTCAAATTCAAATTCAAATTCAAAGCCTATAACTCAGCTTTGGCAGAAAAATGGTATGTGTTCTGAAGGAACAATTCCTATAAGAAGGACCAGAACAAGTGACATATTAAGGGCTAGCTCTGTTCAAAATTTTGGAAAGAAGAAGCATAAAAGTACTCCTCAACCAAAACCTGCAAAACCTCTACCTGACATCTTGACTCAGAGTGGTCATCAGCATGCCATAGCTTATGTGGAGGGAGGTGAATTCTATGGAGCAAAGGCGACCATAAACGTTTGGGACCCAAAAATTCAGCAACCTAATGAATTCAGTCTGTCTCAAATTTGGATTTTGGCTGGTGCCTTTGGTCAAGATCTCAACAGCATTGAAGCAGGCTGGCAGGTCAGCCCTGATTTGTATGGAGACAATAACACTAGACTCTTCACTTATTGGACAAGTGATGCATATCAAGCAACTGGTTGTTATAATCTCCTTTGTTCTGGCTTTATTCAAATTAACAATGGTATAGCCCTTGGAGCAAGTATATCCCCACTTTCTAACTATGGTTCTTCCCAATATGATATAAGCATCTTGGTCTGGAAGGACCCAAAAGAGGGAAACTGGTGGATGCAATTTGGAAATGACCATGTTCTAGGATACTGGCCAGCTCCTTTATTCTCATACCTAACTGAGAGTGCCTCAATGATTGAATGGGGTGGTGAAGTAGTGAACTCTGAGTCTGATGGACAGCACACATCAACACAAATGGGAAGTGGACATTTTCCTGATGAAGGTTTTGGCAAGGCTAGTTATTTCAAAAATATTCAGGTTGTTGATGGTGACAACAAGTTAAGGGCTCCAAAAGATCTTGGTACTTACACTGAAAAAGATAACTGTTATAATGTCAAAACTGGTAGTGCTGGTGATTGGGGCACCTACTTCTATTATGGTGGTCCGGGTCGAAACCCTAACTGTCCTTGA
Protein sequence

>Msa0327750

MEANFGLCFCLLVLLVPFTCAGRATISTTTGNGGGESGGSIQKLEVKKHLKNLNRPPVKSIKSPDGDIIDCVHVSHQPAFDHPDLKDHKIQMRPNFHPEGKTFEESKVSSNSNSNSKPITQLWQKNGMCSEGTIPIRRTRTSDILRASSVQNFGKKKHKSTPQPKPAKPLPDILTQSGHQHAIAYVEGGEFYGAKATINVWDPKIQQPNEFSLSQIWILAGAFGQDLNSIEAGWQVSPDLYGDNNTRLFTYWTSDAYQATGCYNLLCSGFIQINNGIALGASISPLSNYGSSQYDISILVWKDPKEGNWWMQFGNDHVLGYWPAPLFSYLTESASMIEWGGEVVNSESDGQHTSTQMGSGHFPDEGFGKASYFKNIQVVDGDNKLRAPKDLGTYTEKDNCYNVKTGSAGDWGTYFYYGGPGRNPNCP*