AlfalfaGEDB | Msa1206230

M. sativa cultivar zhongmu-4 / Msa1206230

KEGG: K08494
GO: -
PFAM: PF03908.16

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1206230	sp\|Q944A9\|NPS11_ARATH	75.564	266	59	3	1	262	1	264	3.12e-134	382

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1206230	A0A072UPG8	99.620	263	1	0	1	263	1	263	0.0	533

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0001680	Msa1206230	0.815653	8.279089e-52	-8.615850e-47
Msa0012940	Msa1206230	0.818461	1.936881e-52	-8.615850e-47
Msa0035120	Msa1206230	0.801307	9.579124e-49	-8.615850e-47
Msa0052870	Msa1206230	0.818223	2.192568e-52	-8.615850e-47
Msa0075300	Msa1206230	0.800341	1.508500e-48	-8.615850e-47
Msa0077340	Msa1206230	0.819783	9.689594e-53	-8.615850e-47
Msa0081520	Msa1206230	0.809874	1.525108e-50	-8.615850e-47
Msa0093850	Msa1206230	0.832508	9.084806e-56	-8.615850e-47
Msa0098470	Msa1206230	0.845113	4.972330e-59	-8.615850e-47
Msa0099230	Msa1206230	0.851056	1.137563e-60	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1206230	MtrunA17_Chr4g0055321	99.620	263	1	0	1	263	1	263	0.0	533
Msa1206230	MtrunA17_Chr2g0302561	65.370	257	84	2	7	260	7	261	1.89e-110	319
Msa1206230	MtrunA17_Chr4g0069271	64.202	257	87	2	7	260	7	261	2.16e-105	306

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1206230	AT2G35190.1	75.564	266	59	3	1	262	1	264	3.17e-135	382
Msa1206230	AT3G17440.1	63.953	258	87	2	7	260	7	262	1.15e-107	313
Msa1206230	AT1G48240.1	63.178	258	89	2	7	260	7	262	4.48e-106	308
Msa1206230	AT1G48240.2	63.380	213	72	2	52	260	1	211	3.26e-84	251
Msa1206230	AT3G17440.2	61.353	207	76	1	7	209	7	213	4.33e-84	251

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Find 73 sgRNAs with CRISPR-Local

Find 268 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGAATAAACAAAACATTGT+TGG	0.436069	8_1:+63201647	None:intergenic
AAGCCATTGAGAGGGGAAAA+AGG	0.306993	8_1:-63199141	Msa1206230:CDS
AAGCGAATTGAACTCTTTGA+GGG	0.501546	8_1:-63200026	Msa1206230:CDS
AAGGACATTCGCGACATTCC+AGG	0.392891	8_1:-63197190	Msa1206230:CDS
AAGGGATCAAAAGCGCGTTT+AGG	0.389328	8_1:+63201568	None:intergenic
AATAAATGCAGCACTCTGTT+TGG	0.387247	8_1:-63201543	Msa1206230:five_prime_UTR
AATGAACAGCTAATGGATCA+TGG	0.468547	8_1:-63199190	Msa1206230:CDS
AATGTCAAATGAACAGCTAA+TGG	0.436817	8_1:-63199197	Msa1206230:CDS
AATTGCTATCATTATTGTAA+AGG	0.494738	8_1:-63197825	Msa1206230:intron
AATTGGTCAAGGAAATCGGT+AGG	0.642065	8_1:-63198001	Msa1206230:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TAAACAAAACATTGTTGGGT+CGG	+	chr8_1:63196766-63196785	None:intergenic	30.0%
GATTATTGGATTCTGTAATG+TGG	+	chr8_1:63196917-63196936	None:intergenic	30.0%
TGATTGATTACCGTTGAAAA+TGG	-	chr8_1:63197020-63197039	Msa1206230:three_prime_UTR	30.0%
CGGATGCATTTATATAAAAG+TGG	-	chr8_1:63197590-63197609	Msa1206230:intron	30.0%
AATTTAGGCTCATCTAAAAG+TGG	-	chr8_1:63197784-63197803	Msa1206230:intron	30.0%
CATGAAGTAGCAAAATTAAG+GGG	+	chr8_1:63198119-63198138	None:intergenic	30.0%
GAACAAAATGAGAGATTCTA+AGG	+	chr8_1:63198280-63198299	None:intergenic	30.0%
CATTATTTCATATTGGGAGA+TGG	-	chr8_1:63198784-63198803	Msa1206230:intron	30.0%
TGTTTCAGCTATTGTTTGAA+GGG	-	chr8_1:63198986-63199005	Msa1206230:intron	30.0%
ATTAAATCTTCTTTGGTCAG+TGG	+	chr8_1:63199151-63199170	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_1	gene	63196639	63201798	63196639	ID=Msa1206230;Name=Msa1206230
chr8_1	mRNA	63196639	63201798	63196639	ID=Msa1206230-mRNA-1;Parent=Msa1206230;Name=Msa1206230-mRNA-1;_AED=0.13;_eAED=0.13;_QI=400\|1\|1\|1\|1\|1\|10\|487\|266
chr8_1	exon	63196639	63197230	63196639	ID=Msa1206230-mRNA-1:exon:20502;Parent=Msa1206230-mRNA-1
chr8_1	exon	63197826	63197900	63197826	ID=Msa1206230-mRNA-1:exon:20501;Parent=Msa1206230-mRNA-1
chr8_1	exon	63197998	63198090	63197998	ID=Msa1206230-mRNA-1:exon:20500;Parent=Msa1206230-mRNA-1
chr8_1	exon	63198191	63198247	63198191	ID=Msa1206230-mRNA-1:exon:20499;Parent=Msa1206230-mRNA-1
chr8_1	exon	63199141	63199220	63199141	ID=Msa1206230-mRNA-1:exon:20498;Parent=Msa1206230-mRNA-1
chr8_1	exon	63199982	63200070	63199982	ID=Msa1206230-mRNA-1:exon:20497;Parent=Msa1206230-mRNA-1
chr8_1	exon	63200185	63200222	63200185	ID=Msa1206230-mRNA-1:exon:20496;Parent=Msa1206230-mRNA-1
chr8_1	exon	63200373	63200466	63200373	ID=Msa1206230-mRNA-1:exon:20495;Parent=Msa1206230-mRNA-1
chr8_1	exon	63201130	63201222	63201130	ID=Msa1206230-mRNA-1:exon:20494;Parent=Msa1206230-mRNA-1
chr8_1	exon	63201322	63201798	63201322	ID=Msa1206230-mRNA-1:exon:20493;Parent=Msa1206230-mRNA-1
chr8_1	five_prime_UTR	63201399	63201798	63201399	ID=Msa1206230-mRNA-1:five_prime_utr;Parent=Msa1206230-mRNA-1
chr8_1	CDS	63201322	63201398	63201322	ID=Msa1206230-mRNA-1:cds;Parent=Msa1206230-mRNA-1
chr8_1	CDS	63201130	63201222	63201130	ID=Msa1206230-mRNA-1:cds;Parent=Msa1206230-mRNA-1
chr8_1	CDS	63200373	63200466	63200373	ID=Msa1206230-mRNA-1:cds;Parent=Msa1206230-mRNA-1
chr8_1	CDS	63200185	63200222	63200185	ID=Msa1206230-mRNA-1:cds;Parent=Msa1206230-mRNA-1
chr8_1	CDS	63199982	63200070	63199982	ID=Msa1206230-mRNA-1:cds;Parent=Msa1206230-mRNA-1
chr8_1	CDS	63199141	63199220	63199141	ID=Msa1206230-mRNA-1:cds;Parent=Msa1206230-mRNA-1
chr8_1	CDS	63198191	63198247	63198191	ID=Msa1206230-mRNA-1:cds;Parent=Msa1206230-mRNA-1
chr8_1	CDS	63197998	63198090	63197998	ID=Msa1206230-mRNA-1:cds;Parent=Msa1206230-mRNA-1
chr8_1	CDS	63197826	63197900	63197826	ID=Msa1206230-mRNA-1:cds;Parent=Msa1206230-mRNA-1
chr8_1	CDS	63197126	63197230	63197126	ID=Msa1206230-mRNA-1:cds;Parent=Msa1206230-mRNA-1
chr8_1	three_prime_UTR	63196639	63197125	63196639	ID=Msa1206230-mRNA-1:three_prime_utr;Parent=Msa1206230-mRNA-1

Gene Sequence

>Msa1206230

ATGGATCAGTTGTCGGCGATCAGCGAGGATCTGGCGGAGATCGACGGACGCGTCGCCGACAATTTCCGCGCTCTATCAAATGGATTTCAGAAGCTGGAGAAAATTAAGGATGCGAGTAGAAAGAGCAGGCAATTGGAAGAACTCACCGAGAAAATGCGAGAGTGTAAAGGGCTTATCAAAGAGTTTGATAAAGAAGTCAAAGCTTTAGAGGCTAGATTTGATAGAGAAACAACCAAATTTTTGAATGAGAAAAAGCAGTCAATGATCAAAGAATTGAATTCATATGTTGCTTTGAAAAAACAATACGCTTCAAATATTGAGAATAAGCGAATTGAACTCTTTGAGGGACCCAATGAAGGCGACACAGAAGAAAATGTCTTGCTAGCTTCCGCAATGTCAAATGAACAGCTAATGGATCATGGGAATCGGATGATGGATGACACAGATCAAGCCATTGAGAGGGGAAAAAGGATTGTTCAAGACACGGTAAATATTGGTACTGAGACTGCTGCAGCTCTTAAGGCACAGACGGAACAAATGAGCAGAGTTGTCAATGAGCTGGATTCTATCCATTTTTCTATAAAGAAAGCATCTAAATTGGTCAAGGAAATCGGTAGGCAGGTTGCTACTGACAAGTGTATTATGGCATTACTTTTCCTCATCGTCATTGGAGTAATTGCTATCATTATTGTAAAGCTTGTACATCCAGAGAACAAGGACATTCGCGACATTCCAGGATTAGCTCCTCCTGTTGTCACGAACAACAGAAGACTGCTTTGGAATCACAGTCGATTATTTTAA

Protein sequence

>Msa1206230

MDQLSAISEDLAEIDGRVADNFRALSNGFQKLEKIKDASRKSRQLEELTEKMRECKGLIKEFDKEVKALEARFDRETTKFLNEKKQSMIKELNSYVALKKQYASNIENKRIELFEGPNEGDTEENVLLASAMSNEQLMDHGNRMMDDTDQAIERGKRIVQDTVNIGTETAAALKAQTEQMSRVVNELDSIHFSIKKASKLVKEIGRQVATDKCIMALLFLIVIGVIAIIIVKLVHPENKDIRDIPGLAPPVVTNNRRLLWNHSRLF*