AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0296250

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0296250 MtrunA17_Chr2g0315551 99.539 217 1 0 1 217 1 217 2.02e-160 441
Msa0296250 MtrunA17_Chr2g0319951 82.110 218 38 1 1 217 1 218 8.35e-128 359
Msa0296250 MtrunA17_Chr2g0283131 78.539 219 43 3 1 217 1 217 1.50e-125 353
Msa0296250 MtrunA17_Chr4g0046261 78.082 219 44 3 1 217 1 217 4.70e-125 352
Msa0296250 MtrunA17_Chr4g0040781 76.712 219 47 3 1 217 1 217 1.19e-124 351
Msa0296250 MtrunA17_Chr2g0325091 75.472 212 49 3 7 217 5 214 6.31e-117 332
Msa0296250 MtrunA17_Chr4g0032201 68.224 214 67 1 4 217 3 215 3.37e-109 312
Msa0296250 MtrunA17_Chr3g0112681 65.421 214 74 0 3 216 2 215 4.11e-105 301
Msa0296250 MtrunA17_Chr2g0276941 67.925 212 67 1 7 217 9 220 4.68e-103 296
Msa0296250 MtrunA17_Chr8g0381841 59.641 223 72 3 6 217 10 225 8.41e-93 271
Msa0296250 MtrunA17_Chr1g0174281 60.952 210 77 3 11 215 13 222 2.95e-92 269
Msa0296250 MtrunA17_Chr7g0231131 59.420 207 82 1 11 215 13 219 8.20e-91 266
Msa0296250 MtrunA17_Chr3g0123821 59.174 218 85 4 2 215 4 221 1.76e-90 265
Msa0296250 MtrunA17_Chr6g0482721 57.619 210 89 0 7 216 6 215 3.85e-86 254
Msa0296250 MtrunA17_Chr8g0346421 55.963 218 88 3 4 216 17 231 1.61e-83 248
Msa0296250 MtrunA17_Chr7g0275991 54.167 216 95 1 5 216 12 227 4.09e-82 244
Msa0296250 MtrunA17_Chr7g0214241 65.116 172 60 0 12 183 14 185 4.72e-81 241
Msa0296250 MtrunA17_Chr8g0351861 52.423 227 97 4 1 216 1 227 5.58e-80 238
Msa0296250 MtrunA17_Chr1g0189361 53.774 212 92 2 11 216 12 223 4.65e-78 233
Msa0296250 MtrunA17_Chr8g0392931 55.450 211 93 1 7 216 8 218 9.88e-78 232
Msa0296250 MtrunA17_Chr7g0234201 61.628 172 66 0 12 183 12 183 1.86e-76 229
Msa0296250 MtrunA17_Chr2g0290891 46.009 213 105 2 10 216 3 211 5.45e-64 197
Msa0296250 MtrunA17_Chr6g0458081 53.293 167 78 0 10 176 3 169 9.21e-62 191
Msa0296250 MtrunA17_Chr4g0055651 45.972 211 104 2 7 217 2 202 2.32e-60 187
Msa0296250 MtrunA17_Chr8g0335471 47.619 210 99 3 9 217 4 203 2.62e-59 185
Msa0296250 MtrunA17_Chr7g0250531 46.411 209 102 2 9 217 4 202 6.21e-59 184
Msa0296250 MtrunA17_Chr8g0349711 45.024 211 107 3 12 216 5 212 2.21e-58 183
Msa0296250 MtrunA17_Chr1g0182321 43.925 214 115 2 5 217 7 216 1.76e-55 176
Msa0296250 MtrunA17_Chr1g0212521 44.231 208 111 2 9 215 11 214 3.00e-55 175
Msa0296250 MtrunA17_Chr1g0182301 43.458 214 116 2 5 217 7 216 5.99e-55 174
Msa0296250 MtrunA17_Chr4g0030911 45.070 213 101 5 9 215 11 213 2.53e-54 172
Msa0296250 MtrunA17_Chr7g0263071 50.898 167 81 1 9 174 11 177 1.74e-53 171
Msa0296250 MtrunA17_Chr8g0349681 45.894 207 109 3 10 216 381 584 2.53e-53 180
Msa0296250 MtrunA17_Chr8g0349681 50.641 156 77 0 12 167 12 167 2.01e-49 169
Msa0296250 MtrunA17_Chr8g0349681 46.108 167 90 0 10 176 172 338 3.93e-49 169
Msa0296250 MtrunA17_Chr2g0276931 41.905 210 113 2 6 215 2 202 2.80e-53 170
Msa0296250 MtrunA17_Chr1g0199331 47.239 163 85 1 15 176 33 195 2.62e-50 162
Msa0296250 MtrunA17_Chr3g0109721 39.806 206 103 3 15 216 12 200 1.46e-48 157
Msa0296250 MtrunA17_Chr2g0327911 45.506 178 93 3 1 174 1 178 3.90e-48 157
Msa0296250 MtrunA17_Chr5g0433561 39.806 206 103 3 15 216 12 200 1.23e-47 155
Msa0296250 MtrunA17_Chr8g0384311 41.148 209 112 5 9 215 9 208 1.17e-46 153
Msa0296250 MtrunA17_Chr7g0265001 44.498 209 107 4 10 216 13 214 4.82e-46 152
Msa0296250 MtrunA17_Chr1g0159481 45.833 168 88 3 9 174 13 179 5.30e-45 149
Msa0296250 MtrunA17_Chr1g0180291 43.269 208 108 5 10 215 11 210 1.34e-44 148
Msa0296250 MtrunA17_Chr1g0197331 41.589 214 113 5 6 216 8 212 1.37e-44 148
Msa0296250 MtrunA17_Chr8g0390561 41.250 160 94 0 15 174 11 170 1.86e-43 144
Msa0296250 MtrunA17_Chr3g0130271 40.566 212 110 8 9 215 8 208 4.35e-43 144
Msa0296250 MtrunA17_Chr5g0400131 37.580 157 98 0 14 170 10 166 1.89e-40 137
Msa0296250 MtrunA17_Chr4g0055081 36.943 157 99 0 14 170 10 166 1.48e-39 135
Msa0296250 MtrunA17_Chr3g0123971 35.669 157 101 0 14 170 10 166 1.98e-39 134
Msa0296250 MtrunA17_Chr8g0382221 35.669 157 101 0 14 170 10 166 2.24e-39 134
Msa0296250 MtrunA17_Chr6g0466301 38.788 165 94 3 13 170 8 172 6.94e-36 125
Msa0296250 MtrunA17_Chr6g0465491 38.788 165 94 3 13 170 8 172 6.94e-36 125
Msa0296250 MtrunA17_Chr8g0383261 34.848 198 112 4 13 193 12 209 1.05e-35 125
Msa0296250 MtrunA17_Chr4g0034871 33.971 209 119 7 13 214 8 204 1.01e-34 122
Msa0296250 MtrunA17_Chr2g0331691 33.971 209 120 5 13 214 10 207 1.59e-34 122
Msa0296250 MtrunA17_Chr1g0201121 38.889 162 92 4 13 167 8 169 5.65e-34 120
Msa0296250 MtrunA17_Chr4g0004661 33.493 209 121 5 13 214 64 261 1.03e-33 121
Msa0296250 MtrunA17_Chr6g0465471 32.143 196 109 6 28 214 5 185 3.88e-25 97.8
Msa0296250 MtrunA17_Chr6g0466161 31.633 196 110 6 28 214 5 185 1.71e-24 96.3
Msa0296250 MtrunA17_Chr3g0138121 32.468 154 101 2 14 167 14 164 1.42e-23 94.0
Msa0296250 MtrunA17_Chr3g0138101 32.468 154 101 2 14 167 14 164 1.52e-23 94.0
Msa0296250 MtrunA17_Chr3g0138111 32.468 154 101 2 14 167 14 164 1.70e-23 94.0
Msa0296250 MtrunA17_Chr3g0117931 30.387 181 112 5 13 181 8 186 5.61e-21 87.0
Msa0296250 MtrunA17_Chr4g0046211 37.500 120 72 3 13 131 6 123 1.66e-20 84.0
Msa0296250 MtrunA17_Chr4g0037021 38.333 120 71 3 13 131 6 123 6.12e-20 84.0
Msa0296250 MtrunA17_Chr6g0486041 36.364 121 74 3 13 132 6 124 2.86e-19 82.4
Msa0296250 MtrunA17_Chr2g0325101 36.667 120 73 3 13 131 6 123 3.13e-19 82.0
Msa0296250 MtrunA17_Chr5g0405791 35.833 120 74 3 13 131 6 123 7.38e-19 80.9
Msa0296250 MtrunA17_Chr8g0371501 35.833 120 74 3 13 131 6 123 2.72e-18 79.3
Msa0296250 MtrunA17_Chr6g0466431 34.343 99 56 1 13 111 8 97 7.90e-17 73.2
Msa0296250 MtrunA17_Chr6g0465611 34.343 99 56 1 13 111 8 97 7.90e-17 73.2
Msa0296250 MtrunA17_Chr1g0178291 33.526 173 105 5 10 182 428 590 3.39e-15 73.9
Msa0296250 MtrunA17_Chr5g0443631 28.415 183 118 5 3 177 95 272 2.43e-14 70.1
Msa0296250 MtrunA17_Chr1g0182951 60.465 43 17 0 122 164 1 43 1.93e-11 57.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0296250 AT1G16920.1 88.018 217 25 1 1 217 1 216 1.77e-141 394
Msa0296250 AT5G45750.1 85.714 217 30 1 1 217 1 216 2.05e-137 384
Msa0296250 AT4G18800.1 83.410 217 33 2 1 217 1 214 2.41e-134 376
Msa0296250 AT1G06400.1 78.341 217 46 1 1 217 1 216 9.62e-128 359
Msa0296250 AT5G60860.1 76.256 219 48 3 1 217 1 217 3.83e-124 350
Msa0296250 AT3G15060.1 75.799 219 49 3 1 217 1 217 3.79e-123 348
Msa0296250 AT4G18430.1 72.936 218 55 2 1 216 1 216 4.76e-121 342
Msa0296250 AT1G28550.1 71.689 219 59 2 1 217 1 218 2.47e-120 340
Msa0296250 AT2G33870.1 71.233 219 60 2 1 217 1 218 1.38e-117 333
Msa0296250 AT1G07410.1 68.545 213 65 2 5 217 4 214 2.39e-107 308
Msa0296250 AT1G09630.1 68.692 214 65 2 5 217 4 216 2.99e-107 307
Msa0296250 AT5G59150.1 67.130 216 68 2 4 217 3 217 1.99e-102 295
Msa0296250 AT3G46830.1 67.130 216 68 2 4 217 3 217 1.70e-101 293
Msa0296250 AT3G46830.2 67.130 216 68 2 4 217 3 217 1.70e-101 293
Msa0296250 AT5G47960.1 63.810 210 73 1 11 217 13 222 1.43e-94 276
Msa0296250 AT3G12160.1 60.577 208 78 2 11 215 13 219 1.98e-92 270
Msa0296250 AT4G39990.1 60.185 216 70 3 11 217 15 223 7.73e-91 266
Msa0296250 AT5G65270.1 61.033 213 76 3 11 217 15 226 1.91e-89 263
Msa0296250 AT5G47520.1 56.757 222 90 4 1 217 1 221 2.10e-84 250
Msa0296250 AT2G43130.1 55.140 214 91 3 6 216 2 213 7.75e-81 240
Msa0296250 AT2G31680.1 54.839 217 80 3 11 217 10 218 4.05e-79 236
Msa0296250 AT1G18200.1 51.982 227 97 2 1 215 1 227 9.09e-78 233
Msa0296250 AT1G05810.1 55.814 215 80 4 11 217 10 217 9.10e-78 233
Msa0296250 AT3G07410.1 61.453 179 69 0 3 181 2 180 2.02e-77 232
Msa0296250 AT1G05810.2 55.814 215 80 4 11 217 56 263 2.68e-77 233
Msa0296250 AT1G73640.1 52.814 231 93 3 1 215 1 231 3.15e-77 232
Msa0296250 AT1G01200.1 54.460 213 88 4 11 216 26 236 7.12e-75 226
Msa0296250 AT5G59150.2 61.818 165 60 2 55 217 3 166 1.31e-67 205
Msa0296250 AT4G17170.1 46.009 213 105 2 10 216 3 211 9.35e-64 197
Msa0296250 AT4G17160.1 47.343 207 103 2 10 215 3 204 1.69e-62 194
Msa0296250 AT4G35860.1 53.293 167 78 0 10 176 3 169 1.43e-61 191
Msa0296250 AT1G02130.1 47.170 212 101 3 7 217 2 203 4.63e-60 187
Msa0296250 AT5G47200.1 45.972 211 104 2 7 217 2 202 1.03e-58 184
Msa0296250 AT4G17530.1 45.498 211 105 2 7 217 2 202 1.34e-58 184
Msa0296250 AT3G11730.1 43.750 208 110 1 8 215 3 203 2.09e-57 181
Msa0296250 AT5G03520.1 45.161 217 113 2 1 216 3 214 2.42e-55 176
Msa0296250 AT3G46060.2 51.534 163 78 1 9 170 11 173 2.98e-53 170
Msa0296250 AT3G46060.3 51.534 163 78 1 9 170 11 173 2.98e-53 170
Msa0296250 AT3G46060.1 51.534 163 78 1 9 170 11 173 2.98e-53 170
Msa0296250 AT3G09900.1 42.857 217 120 2 1 216 3 216 7.30e-53 169
Msa0296250 AT5G59840.1 50.299 167 82 1 9 174 11 177 1.09e-52 169
Msa0296250 AT5G45130.1 45.109 184 95 1 15 198 12 189 3.98e-52 167
Msa0296250 AT3G53610.2 49.701 167 83 1 9 174 11 177 6.51e-52 167
Msa0296250 AT3G53610.3 49.701 167 83 1 9 174 11 177 6.51e-52 167
Msa0296250 AT3G53610.1 49.701 167 83 1 9 174 11 177 6.51e-52 167
Msa0296250 AT4G19640.1 46.626 163 87 0 15 177 12 174 2.69e-51 165
Msa0296250 AT5G45130.2 45.055 182 94 1 17 198 22 197 4.21e-51 165
Msa0296250 AT1G43890.3 45.545 202 102 5 1 196 1 200 1.05e-50 164
Msa0296250 AT1G43890.1 45.545 202 102 5 1 196 1 200 1.05e-50 164
Msa0296250 AT1G43890.2 45.545 202 102 5 1 196 1 200 1.05e-50 164
Msa0296250 AT3G54840.1 47.853 163 84 1 15 176 35 197 1.20e-49 160
Msa0296250 AT3G54840.3 47.531 162 84 1 16 176 2 163 3.18e-49 159
Msa0296250 AT3G54840.2 49.351 154 77 1 15 167 35 188 3.58e-48 157
Msa0296250 AT5G03530.2 43.119 218 114 5 1 216 1 210 1.02e-46 154
Msa0296250 AT5G03530.1 43.119 218 114 5 1 216 1 210 1.02e-46 154
Msa0296250 AT4G35860.2 51.639 122 59 0 55 176 2 123 2.56e-42 141
Msa0296250 AT2G22290.1 36.306 157 100 0 14 170 10 166 3.71e-39 134
Msa0296250 AT5G03520.2 41.765 170 93 2 48 216 40 204 1.92e-38 132
Msa0296250 AT5G10260.1 35.669 157 101 0 14 170 10 166 2.47e-38 132
Msa0296250 AT4G39890.1 36.709 158 99 1 14 170 10 167 3.16e-38 132
Msa0296250 AT4G19640.3 46.565 131 70 0 15 145 12 142 3.85e-38 131
Msa0296250 AT2G44610.1 36.306 157 100 0 14 170 10 166 8.41e-38 130
Msa0296250 AT4G19640.2 47.200 125 66 0 15 139 12 136 1.39e-37 128
Msa0296250 AT3G09910.3 41.414 198 108 4 1 196 1 192 3.62e-37 129
Msa0296250 AT3G09910.1 41.414 198 108 4 1 196 1 192 3.62e-37 129
Msa0296250 AT3G09910.2 41.414 198 108 4 1 196 1 192 3.62e-37 129
Msa0296250 AT1G22740.1 40.123 162 90 3 13 167 8 169 3.77e-37 129
Msa0296250 AT5G64990.1 35.404 161 104 0 14 174 8 168 5.19e-37 129
Msa0296250 AT5G46025.1 58.491 106 43 1 75 179 6 111 9.88e-37 125
Msa0296250 AT3G09910.5 41.026 195 107 4 1 188 1 194 5.55e-36 125
Msa0296250 AT3G09910.4 41.026 195 107 4 1 188 1 194 5.55e-36 125
Msa0296250 AT3G18820.1 35.450 189 107 4 13 194 8 188 5.71e-35 123
Msa0296250 AT2G21880.1 38.788 165 94 3 13 170 9 173 7.61e-35 123
Msa0296250 AT1G52280.1 38.095 168 96 4 13 173 8 174 1.76e-34 122
Msa0296250 AT5G64990.2 33.929 168 104 1 14 174 8 175 2.74e-34 122
Msa0296250 AT3G16100.1 37.654 162 94 3 13 167 8 169 3.80e-33 119
Msa0296250 AT1G49300.2 36.527 167 99 3 13 172 8 174 8.04e-33 118
Msa0296250 AT1G49300.1 36.527 167 99 3 13 172 8 174 8.04e-33 118
Msa0296250 AT4G09720.1 35.758 165 99 2 13 170 8 172 2.04e-32 117
Msa0296250 AT5G39620.1 34.211 190 114 4 14 195 6 192 1.15e-31 115
Msa0296250 AT4G09720.4 34.118 170 100 2 13 170 8 177 4.41e-31 113
Msa0296250 AT4G09720.3 32.955 176 100 2 13 170 8 183 6.36e-30 110
Msa0296250 AT5G55080.1 35.256 156 94 3 14 167 14 164 2.31e-27 104
Msa0296250 AT2G21880.4 35.811 148 88 3 30 170 1 148 1.13e-25 99.0
Msa0296250 AT2G21880.3 35.811 148 88 3 30 170 1 148 1.13e-25 99.0
Msa0296250 AT2G21880.2 33.939 165 94 4 13 170 9 165 6.10e-25 97.4
Msa0296250 AT5G20020.1 32.468 154 101 2 14 167 14 164 2.09e-23 94.0
Msa0296250 AT5G55190.1 32.468 154 101 2 14 167 14 164 2.16e-23 94.0
Msa0296250 AT5G20010.1 32.468 154 101 2 14 167 14 164 2.18e-23 94.0
Msa0296250 AT4G08190.1 63.636 66 21 1 102 167 65 127 8.88e-22 87.0
Msa0296250 AT2G44690.1 32.222 180 108 5 13 180 18 195 1.32e-21 89.0
Msa0296250 AT4G09720.6 34.815 135 81 2 40 167 1 135 1.45e-21 87.8
Msa0296250 AT4G09720.5 34.815 135 81 2 40 167 1 135 1.45e-21 87.8
Msa0296250 AT4G09720.2 34.815 135 81 2 40 167 1 135 1.45e-21 87.8
Msa0296250 AT4G35020.1 34.132 167 96 5 13 167 6 170 1.61e-20 85.9
Msa0296250 AT4G35020.2 34.132 167 96 5 13 167 6 170 1.61e-20 85.9
Msa0296250 AT4G35020.3 34.132 167 96 5 13 167 6 170 1.61e-20 85.9
Msa0296250 AT4G28950.1 29.121 182 117 5 13 184 6 185 7.91e-20 84.3
Msa0296250 AT3G48040.1 29.949 197 120 6 13 197 8 198 1.94e-19 83.2
Msa0296250 AT1G75840.1 31.737 167 100 5 13 167 6 170 5.33e-19 81.6
Msa0296250 AT5G45970.1 37.500 120 72 3 13 131 6 123 7.82e-19 81.3
Msa0296250 AT1G20090.1 35.833 120 74 3 13 131 5 122 9.77e-19 80.9
Msa0296250 AT5G62880.1 29.545 176 110 5 13 176 8 181 4.44e-18 79.7
Msa0296250 AT2G17800.2 35.833 120 74 3 13 131 6 123 5.02e-18 79.0
Msa0296250 AT2G17800.1 35.833 120 74 3 13 131 6 123 5.02e-18 79.0
Msa0296250 AT4G35950.1 35.833 120 74 3 13 131 6 123 5.74e-18 79.0
Msa0296250 AT3G51300.1 36.441 118 72 3 15 131 8 123 5.99e-18 79.0
Msa0296250 AT4G35020.4 38.596 114 67 3 15 127 8 119 1.63e-17 76.6
Msa0296250 AT4G35020.5 38.596 114 67 3 15 127 8 119 1.63e-17 76.6
Msa0296250 AT3G48040.2 31.210 157 94 5 13 157 8 162 2.62e-17 76.6
Msa0296250 AT5G62880.2 35.000 120 75 3 13 131 8 125 4.17e-17 76.6

Find 55 sgRNAs with CRISPR-Local

Find 229 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCAACACCAATGGTGGATT+TGG 0.236269 2_3:+49339307 None:intergenic
TGTTGATTCTAAAGTTATTA+AGG 0.259169 2_3:-49339264 Msa0296250:CDS
TGGAAGTAATTAATTCATTA+AGG 0.261287 2_3:-49335580 Msa0296250:three_prime_UTR
GAAAGAATCACTTTATTTCA+TGG 0.263251 2_3:-49336051 Msa0296250:CDS
GATTGGAAACATAATTCATT+TGG 0.342285 2_3:-49335834 Msa0296250:three_prime_UTR
CATGTTTCAGTAGGAGCTGC+TGG 0.345152 2_3:-49335683 Msa0296250:three_prime_UTR
ATTTGTATCTCTCTCACTTT+CGG 0.348472 2_3:+49335780 None:intergenic
GCTGTAGAGGGTGCTGAAAA+TGG 0.383578 2_3:-49335945 Msa0296250:CDS
GAAATAAAGTGATTCTTTCT+CGG 0.391175 2_3:+49336055 None:intergenic
AAGTTATTAAGGCTCAGATC+TGG 0.406315 2_3:-49339253 Msa0296250:CDS
GTTGCTGCTCAAGCTAAGAT+TGG 0.425537 2_3:-49335851 Msa0296250:exon
AACATTGTTGTGATGCTTGT+TGG 0.427166 2_3:-49336137 Msa0296250:CDS
TTTCATGGAAACCTCAGCTC+TGG 0.442500 2_3:-49336036 Msa0296250:CDS
CGATCAGAACTACCTTGAAT+AGG 0.446974 2_3:+49339396 None:intergenic
GGTGAAACGAGAGAGAAGAT+TGG 0.454307 2_3:+49339352 None:intergenic
CGACTATGACTACCTATTCA+AGG 0.457570 2_3:-49339408 Msa0296250:CDS
ATAATTCATTTGGTTGCTAT+CGG 0.460679 2_3:-49335824 Msa0296250:three_prime_UTR
CAGCTTTCTTGCTAACAATA+CGG 0.494643 2_3:+49335964 None:intergenic
CTTCCCATCTTCAGTTGATA+CGG 0.496302 2_3:+49336085 None:intergenic
ATTTGAAGACTTCCAGTGGT+TGG 0.500985 2_3:+49335504 None:intergenic
CTGATCGGCGACTCCGGCGT+TGG 0.508206 2_3:-49339380 Msa0296250:CDS
GCTTACTATCGTGGGGCAGT+TGG 0.519994 2_3:-49336248 Msa0296250:CDS
ATGTTTCAGTAGGAGCTGCT+GGG 0.520718 2_3:-49335682 Msa0296250:three_prime_UTR
TCTGCAAATTGATTCAAGCT+TGG 0.529602 2_3:-49335600 Msa0296250:three_prime_UTR
GACAAGTTTCATGTTTCAGT+AGG 0.531782 2_3:-49335692 Msa0296250:three_prime_UTR
CTTCAGTTGATACGGCTACA+AGG 0.532280 2_3:+49336093 None:intergenic
TAATAGAATCATCCAACCAC+TGG 0.533498 2_3:-49335516 Msa0296250:three_prime_UTR
GTAGTTCTGATCGGCGACTC+CGG 0.534376 2_3:-49339386 Msa0296250:CDS
AGTTATTAAGGCTCAGATCT+GGG 0.536509 2_3:-49339252 Msa0296250:CDS
GGGATACTGCTGGTCAAGAA+AGG 0.537036 2_3:-49339232 Msa0296250:intron
GTTTCAGCTTTGAAGAGAGT+TGG 0.538571 2_3:-49335873 Msa0296250:CDS
AACAAGCATCACAACAATGT+TGG 0.538592 2_3:+49336139 None:intergenic
CTTGAATCCAAATCCACCAT+TGG 0.540714 2_3:-49339314 Msa0296250:CDS
AACGCGTCTGTGCCAGCCAA+AGG 0.543390 2_3:-49335921 Msa0296250:CDS
ACGATAGTAAGCACTTGTAA+TGG 0.555075 2_3:+49336259 None:intergenic
GTAGCCGTATCAACTGAAGA+TGG 0.561123 2_3:-49336089 Msa0296250:CDS
GCTCAGATCTGGGATACTGC+TGG 0.571910 2_3:-49339242 Msa0296250:CDS
ATTACAAGTGCTTACTATCG+TGG 0.574326 2_3:-49336257 Msa0296250:CDS
TTACAAGTGCTTACTATCGT+GGG 0.574573 2_3:-49336256 Msa0296250:CDS
CATGGAAACCTCAGCTCTGG+AGG 0.574684 2_3:-49336033 Msa0296250:CDS
AGCGAATTCAACACCAATGG+TGG 0.577629 2_3:+49339301 None:intergenic
CTATTCAAGGTAGTTCTGAT+CGG 0.585029 2_3:-49339395 Msa0296250:CDS
TGTTAGCAAGAAAGCTGTAG+AGG 0.588403 2_3:-49335958 Msa0296250:CDS
CGACGTTCTCAAATGTTGCA+TGG 0.590652 2_3:+49336198 None:intergenic
TAGCCGTATCAACTGAAGAT+GGG 0.593299 2_3:-49336088 Msa0296250:CDS
GTTAGCAAGAAAGCTGTAGA+GGG 0.598414 2_3:-49335957 Msa0296250:CDS
AAGATTGGATTTGCCAACGC+CGG 0.601803 2_3:+49339367 None:intergenic
CATTTGAGAACGTCGACAGA+TGG 0.604678 2_3:-49336190 Msa0296250:CDS
TGTTTCAGTAGGAGCTGCTG+GGG 0.605790 2_3:-49335681 Msa0296250:three_prime_UTR
CCTTATTTGAAGACTTCCAG+TGG 0.618831 2_3:+49335500 None:intergenic
GTTAGTAGCCTCCAGAGCTG+AGG 0.627901 2_3:+49336025 None:intergenic
TTGATACGGCTACAAGGTGA+CGG 0.654516 2_3:+49336099 None:intergenic
TACAAGTGCTTACTATCGTG+GGG 0.673719 2_3:-49336255 Msa0296250:CDS
AGTAGCGAATTCAACACCAA+TGG 0.690637 2_3:+49339298 None:intergenic
CAACAATGTTGGAATCTGTG+TGG 0.736098 2_3:+49336150 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAATTAATAATTCTATTAG+CGG - chr2_3:49336188-49336207 Msa0296250:CDS 10.0%
!!! AATTTTTATTTTTTTTGCTA+AGG + chr2_3:49336511-49336530 None:intergenic 10.0%
!! AAGAATTTATATTACAAAAT+TGG - chr2_3:49337289-49337308 Msa0296250:intron 10.0%
!!! TTCAGAGATTTTAATTTAAA+TGG - chr2_3:49336084-49336103 Msa0296250:CDS 15.0%
!!! TCTAAAATATGATTTGATTT+TGG - chr2_3:49336535-49336554 Msa0296250:intron 15.0%
!!! TTGTTTTATATATTCTATGA+TGG - chr2_3:49336908-49336927 Msa0296250:intron 15.0%
!! AATATAAATTCTTAAGAGTT+GGG + chr2_3:49337282-49337301 None:intergenic 15.0%
!! TAATATAAATTCTTAAGAGT+TGG + chr2_3:49337283-49337302 None:intergenic 15.0%
!! CTATTCAAATATTTGAAAAT+AGG + chr2_3:49337336-49337355 None:intergenic 15.0%
!! TAAATAAAAACAATGTTACT+CGG + chr2_3:49338418-49338437 None:intergenic 15.0%
!!! TGTTGATTCTAAAGTTATTA+AGG - chr2_3:49335801-49335820 Msa0296250:three_prime_UTR 20.0%
!!! ATGAAGAAGAAATGAATTTT+AGG - chr2_3:49336162-49336181 Msa0296250:CDS 20.0%
!! TTTGTTTAAAAAAGATCAAG+AGG - chr2_3:49336344-49336363 Msa0296250:intron 20.0%
!!! TTTTTGCTAAGGAAAATTTA+GGG + chr2_3:49336500-49336519 None:intergenic 20.0%
!!! TTTTTTGCTAAGGAAAATTT+AGG + chr2_3:49336501-49336520 None:intergenic 20.0%
!! AAGGAATTACAAAAAACTAA+AGG - chr2_3:49336587-49336606 Msa0296250:intron 20.0%
!! AGGAATTACAAAAAACTAAA+GGG - chr2_3:49336588-49336607 Msa0296250:intron 20.0%
!! TCTATAAAAATTTCAAAAGC+TGG + chr2_3:49336629-49336648 None:intergenic 20.0%
!!! TATACTTCAATTTTTTTGAC+TGG - chr2_3:49336745-49336764 Msa0296250:intron 20.0%
!!! ATATCTTATTTAAGAACTGT+TGG - chr2_3:49336931-49336950 Msa0296250:intron 20.0%
!!! TATCTTATTTAAGAACTGTT+GGG - chr2_3:49336932-49336951 Msa0296250:intron 20.0%
!! AATTTATACTAAATAGCGTA+TGG - chr2_3:49337164-49337183 Msa0296250:intron 20.0%
!!! AGAAATGTTTTTATTTTCAC+TGG + chr2_3:49337451-49337470 None:intergenic 20.0%
!!! TTTTGAAAGAAAAAAGTTGA+TGG + chr2_3:49337880-49337899 None:intergenic 20.0%
!! TGATTAAAAAACCAGAATTA+TGG - chr2_3:49338192-49338211 Msa0296250:intron 20.0%
!! GATTAAAAAACCAGAATTAT+GGG - chr2_3:49338193-49338212 Msa0296250:intron 20.0%
!! ATTAAAAAACCAGAATTATG+GGG - chr2_3:49338194-49338213 Msa0296250:intron 20.0%
!!! AGATTTTTACTACTGATAAT+TGG + chr2_3:49338247-49338266 None:intergenic 20.0%
!! TTATCAGTAGTAAAAATCTA+AGG - chr2_3:49338248-49338267 Msa0296250:intron 20.0%
!!! AGTTCTAATACTAATAATAG+GGG + chr2_3:49338299-49338318 None:intergenic 20.0%
!!! GAGTTCTAATACTAATAATA+GGG + chr2_3:49338300-49338319 None:intergenic 20.0%
!!! AGAGTTCTAATACTAATAAT+AGG + chr2_3:49338301-49338320 None:intergenic 20.0%
!!! AAGTTCTAATACTAACAATT+GGG + chr2_3:49338361-49338380 None:intergenic 20.0%
!!! AAAGTTCTAATACTAACAAT+TGG + chr2_3:49338362-49338381 None:intergenic 20.0%
!! TTGTTAGTATTAGAACTTTA+AGG - chr2_3:49338363-49338382 Msa0296250:intron 20.0%
!! AAATAAAAACAATGTTACTC+GGG + chr2_3:49338417-49338436 None:intergenic 20.0%
!!! GTAACATTGTTTTTATTTAG+CGG - chr2_3:49338419-49338438 Msa0296250:intron 20.0%
!! AAACTTACAAATGATAAGTA+TGG - chr2_3:49338464-49338483 Msa0296250:intron 20.0%
!! TGGAAGTAATTAATTCATTA+AGG - chr2_3:49339485-49339504 Msa0296250:five_prime_UTR 20.0%
!! CATTTCTACATTTTGCTTAA+TGG - chr2_3:49335888-49335907 Msa0296250:CDS 25.0%
! ATTTAAATGGTTAAGTGATG+TGG - chr2_3:49336097-49336116 Msa0296250:CDS 25.0%
!! TAAGGAAAATTTAGGGTAAA+GGG + chr2_3:49336493-49336512 None:intergenic 25.0%
!! TGGCAAATACCAATTATTTT+AGG - chr2_3:49336555-49336574 Msa0296250:intron 25.0%
!!! GGCAAATACCAATTATTTTA+GGG - chr2_3:49336556-49336575 Msa0296250:intron 25.0%
! TAATAATAACTAAAGCCTAC+AGG + chr2_3:49336717-49336736 None:intergenic 25.0%
! TATAAATTGTTGTCAAACAG+TGG + chr2_3:49337152-49337171 None:intergenic 25.0%
!! TAGCTGCTATTTAACAATAT+TGG - chr2_3:49337243-49337262 Msa0296250:intron 25.0%
!! TTCTCACATTTTCTTATAGA+AGG - chr2_3:49337376-49337395 Msa0296250:intron 25.0%
! AATATGTAATGAAAGGAACA+TGG + chr2_3:49337772-49337791 None:intergenic 25.0%
! ACACTAGTGTAGTAATATAT+TGG - chr2_3:49337838-49337857 Msa0296250:intron 25.0%
! GTAGTTAATTCTTACTAGTA+GGG + chr2_3:49337997-49338016 None:intergenic 25.0%
! AGTAGTTAATTCTTACTAGT+AGG + chr2_3:49337998-49338017 None:intergenic 25.0%
! GAAGATAATGTAAACTTTGT+TGG + chr2_3:49338079-49338098 None:intergenic 25.0%
! TATTGTTCAATTACTCTTCA+AGG + chr2_3:49338120-49338139 None:intergenic 25.0%
!!! TAACTTGTATTTTTGAGCAA+AGG - chr2_3:49338168-49338187 Msa0296250:intron 25.0%
!! AGTTCTAATACTAACAATTG+GGG + chr2_3:49338360-49338379 None:intergenic 25.0%
! ATCTGTAATCAAACTTACAA+AGG - chr2_3:49338548-49338567 Msa0296250:intron 25.0%
! TAATCAAACTTACAAAGGAT+AGG - chr2_3:49338553-49338572 Msa0296250:intron 25.0%
! TTACAAATCAATCGTAACAA+TGG + chr2_3:49338706-49338725 None:intergenic 25.0%
! CTAAAGTCAGTTACAAAATT+TGG + chr2_3:49338736-49338755 None:intergenic 25.0%
! GAAATAAAGTGATTCTTTCT+CGG + chr2_3:49339013-49339032 None:intergenic 25.0%
!! GAAAGAATCACTTTATTTCA+TGG - chr2_3:49339014-49339033 Msa0296250:intron 25.0%
! GATTGGAAACATAATTCATT+TGG - chr2_3:49339231-49339250 Msa0296250:intron 25.0%
! ATAATTCATTTGGTTGCTAT+CGG - chr2_3:49339241-49339260 Msa0296250:CDS 25.0%
TCAAAGAAGAAGAAGAAGAA+TGG - chr2_3:49335615-49335634 Msa0296250:three_prime_UTR 30.0%
! TTCAAGATTGAACTCGTTTT+TGG + chr2_3:49335737-49335756 None:intergenic 30.0%
! CTAAGGAAAATTTAGGGTAA+AGG + chr2_3:49336494-49336513 None:intergenic 30.0%
!!! TACCAATTATTTTAGGGCAT+TGG - chr2_3:49336562-49336581 Msa0296250:intron 30.0%
AACCAATGCCCTAAAATAAT+TGG + chr2_3:49336567-49336586 None:intergenic 30.0%
!!! TTATTTTAGGGCATTGGTTA+AGG - chr2_3:49336568-49336587 Msa0296250:intron 30.0%
! TTTCTAATTCTTGTTTGACC+TGG - chr2_3:49336681-49336700 Msa0296250:intron 30.0%
! TATGTTTAGAGTCCCATATT+TGG - chr2_3:49336976-49336995 Msa0296250:intron 30.0%
!!! CTTACAAGCTAGTTTTGTAA+GGG - chr2_3:49337045-49337064 Msa0296250:intron 30.0%
! CATTTTCTTATAGAAGGCTA+TGG - chr2_3:49337382-49337401 Msa0296250:intron 30.0%
ATCACACTCTTTCGATATTT+AGG - chr2_3:49337516-49337535 Msa0296250:intron 30.0%
TCACACTCTTTCGATATTTA+GGG - chr2_3:49337517-49337536 Msa0296250:intron 30.0%
!! AGTTTTCCATTTGGAATAGA+GGG + chr2_3:49337659-49337678 None:intergenic 30.0%
!! TAGTTTTCCATTTGGAATAG+AGG + chr2_3:49337660-49337679 None:intergenic 30.0%
! CAAAAGAGTAGTTTTCCATT+TGG + chr2_3:49337668-49337687 None:intergenic 30.0%
CATGTGATGGTTAATGATAT+TGG - chr2_3:49337688-49337707 Msa0296250:intron 30.0%
ATGGTTAATGATATTGGTGT+GGG - chr2_3:49337694-49337713 Msa0296250:intron 30.0%
GGAACATGGAAAATTATAAC+TGG + chr2_3:49337758-49337777 None:intergenic 30.0%
TGGGATGAATATGTAATGAA+AGG + chr2_3:49337779-49337798 None:intergenic 30.0%
! ATGGGGATATTTGTTTCTTT+CGG + chr2_3:49337965-49337984 None:intergenic 30.0%
AAACTTACAAAGGATAGGTA+TGG - chr2_3:49338558-49338577 Msa0296250:intron 30.0%
! AAGATCTATTTTCTCTCCTT+TGG + chr2_3:49339163-49339182 None:intergenic 30.0%
ATTTGTATCTCTCTCACTTT+CGG + chr2_3:49339288-49339307 None:intergenic 30.0%
! TTTATTTGTGCACTGTTTTG+TGG - chr2_3:49339510-49339529 Msa0296250:five_prime_UTR 30.0%
AAGAAGAAGAAGAAGAATGG+CGG - chr2_3:49335618-49335637 Msa0296250:three_prime_UTR 35.0%
!! CTATTCAAGGTAGTTCTGAT+CGG - chr2_3:49335670-49335689 Msa0296250:three_prime_UTR 35.0%
AAGTTATTAAGGCTCAGATC+TGG - chr2_3:49335812-49335831 Msa0296250:three_prime_UTR 35.0%
AGTTATTAAGGCTCAGATCT+GGG - chr2_3:49335813-49335832 Msa0296250:three_prime_UTR 35.0%
ACATCAGCACTACAAACAAA+TGG + chr2_3:49336044-49336063 None:intergenic 35.0%
! GAATTCAGTGTTGTGTCTTT+TGG - chr2_3:49336215-49336234 Msa0296250:CDS 35.0%
TCCATAGTAGCAGTTGAATT+TGG - chr2_3:49336264-49336283 Msa0296250:CDS 35.0%
TCCAAATTCAACTGCTACTA+TGG + chr2_3:49336268-49336287 None:intergenic 35.0%
TAGTAGCAGTTGAATTTGGA+CGG - chr2_3:49336268-49336287 Msa0296250:CDS 35.0%
TTTACCATAGATCGAGTCTT+TGG + chr2_3:49336415-49336434 None:intergenic 35.0%
TCGATCTATGGTAAAAAGAC+AGG - chr2_3:49336420-49336439 Msa0296250:intron 35.0%
! GAAAATTTAGGGTAAAGGGA+TGG + chr2_3:49336489-49336508 None:intergenic 35.0%
GTCTAATCTTCAGTTGAACT+TGG - chr2_3:49336775-49336794 Msa0296250:intron 35.0%
AACCAAGCAAGTTCAAAATG+AGG + chr2_3:49336873-49336892 None:intergenic 35.0%
ACCTTCTCTCAACCAAATAT+GGG + chr2_3:49336991-49337010 None:intergenic 35.0%
TGGATATGAGCTCATTAGTT+GGG - chr2_3:49337012-49337031 Msa0296250:intron 35.0%
! CCTTACAAAACTAGCTTGTA+AGG + chr2_3:49337047-49337066 None:intergenic 35.0%
!!! CCTTACAAGCTAGTTTTGTA+AGG - chr2_3:49337044-49337063 Msa0296250:intron 35.0%
TATACTAAATAGCGTATGGC+AGG - chr2_3:49337168-49337187 Msa0296250:intron 35.0%
GAAGAAACAGAGCGAAAATA+AGG - chr2_3:49337408-49337427 Msa0296250:intron 35.0%
! AACCAAGAGCATGTTTGATT+TGG + chr2_3:49337474-49337493 None:intergenic 35.0%
!! GTTTTCCATTTGGAATAGAG+GGG + chr2_3:49337658-49337677 None:intergenic 35.0%
! AACTACTCTTTTGCATGTGA+TGG - chr2_3:49337675-49337694 Msa0296250:intron 35.0%
GATGGTTAATGATATTGGTG+TGG - chr2_3:49337693-49337712 Msa0296250:intron 35.0%
TGATATTGGTGTGGGTTTAT+AGG - chr2_3:49337702-49337721 Msa0296250:intron 35.0%
ATTGGGTGCAGTGTTTAAAT+GGG + chr2_3:49337798-49337817 None:intergenic 35.0%
! ATATTTGTTTCTTTCGGGGA+GGG + chr2_3:49337959-49337978 None:intergenic 35.0%
! TGGGGATATTTGTTTCTTTC+GGG + chr2_3:49337964-49337983 None:intergenic 35.0%
AAAAAACCAGAATTATGGGG+TGG - chr2_3:49338197-49338216 Msa0296250:intron 35.0%
AAAAACCAGAATTATGGGGT+GGG - chr2_3:49338198-49338217 Msa0296250:intron 35.0%
AAGGCCTAAAGTACAATAAG+CGG - chr2_3:49338516-49338535 Msa0296250:intron 35.0%
ACTTATTGTACTTTAGGCCT+TGG + chr2_3:49338611-49338630 None:intergenic 35.0%
AAGGCCTAAAGTACAATAAG+TGG - chr2_3:49338516-49338535 Msa0296250:intron 35.0%
GACACCACTTATTGTACTTT+AGG + chr2_3:49338617-49338636 None:intergenic 35.0%
ACGATAGTAAGCACTTGTAA+TGG + chr2_3:49338809-49338828 None:intergenic 35.0%
ATTACAAGTGCTTACTATCG+TGG - chr2_3:49338808-49338827 Msa0296250:intron 35.0%
TTACAAGTGCTTACTATCGT+GGG - chr2_3:49338809-49338828 Msa0296250:intron 35.0%
AACAAGCATCACAACAATGT+TGG + chr2_3:49338929-49338948 None:intergenic 35.0%
AACATTGTTGTGATGCTTGT+TGG - chr2_3:49338928-49338947 Msa0296250:intron 35.0%
CAGCTTTCTTGCTAACAATA+CGG + chr2_3:49339104-49339123 None:intergenic 35.0%
GACAAGTTTCATGTTTCAGT+AGG - chr2_3:49339373-49339392 Msa0296250:CDS 35.0%
! TCTGCAAATTGATTCAAGCT+TGG - chr2_3:49339465-49339484 Msa0296250:five_prime_UTR 35.0%
TAATAGAATCATCCAACCAC+TGG - chr2_3:49339549-49339568 Msa0296250:five_prime_UTR 35.0%
AGAAGAAGAAGAAGAATGGC+GGG - chr2_3:49335619-49335638 Msa0296250:three_prime_UTR 40.0%
CGACTATGACTACCTATTCA+AGG - chr2_3:49335657-49335676 Msa0296250:three_prime_UTR 40.0%
CGATCAGAACTACCTTGAAT+AGG + chr2_3:49335672-49335691 None:intergenic 40.0%
CTTGAATCCAAATCCACCAT+TGG - chr2_3:49335751-49335770 Msa0296250:three_prime_UTR 40.0%
!! TTCAACACCAATGGTGGATT+TGG + chr2_3:49335761-49335780 None:intergenic 40.0%
AGTAGCGAATTCAACACCAA+TGG + chr2_3:49335770-49335789 None:intergenic 40.0%
TAACAACAGATCACGATCGA+CGG + chr2_3:49335922-49335941 None:intergenic 40.0%
!! TAAACTTAGTAGCACCGACA+CGG - chr2_3:49335951-49335970 Msa0296250:CDS 40.0%
ATCTCCAAAGACTCGATCTA+TGG - chr2_3:49336408-49336427 Msa0296250:intron 40.0%
AGGCAGAGAAGACAATCTAT+CGG + chr2_3:49336454-49336473 None:intergenic 40.0%
ATAGATTGTCTTCTCTGCCT+TGG - chr2_3:49336454-49336473 Msa0296250:intron 40.0%
! TTCTTGTTTGACCTGGTTAG+CGG - chr2_3:49336688-49336707 Msa0296250:intron 40.0%
ATGAGGCTTATGCACGAAAT+AGG + chr2_3:49336856-49336875 None:intergenic 40.0%
!! AGCCTCATTTTGAACTTGCT+TGG - chr2_3:49336868-49336887 Msa0296250:intron 40.0%
TAGACCTCTCCAATATCAAG+CGG + chr2_3:49336958-49336977 None:intergenic 40.0%
TCCCATATTTGGTTGAGAGA+AGG - chr2_3:49336987-49337006 Msa0296250:intron 40.0%
GACCTTCTCTCAACCAAATA+TGG + chr2_3:49336992-49337011 None:intergenic 40.0%
TATTTGGTTGAGAGAAGGTC+TGG - chr2_3:49336992-49337011 Msa0296250:intron 40.0%
CTGGATATGAGCTCATTAGT+TGG - chr2_3:49337011-49337030 Msa0296250:intron 40.0%
CTCTGTAGAGTAGCGAAATT+TGG + chr2_3:49337209-49337228 None:intergenic 40.0%
CAAATTTCGCTACTCTACAG+AGG - chr2_3:49337207-49337226 Msa0296250:intron 40.0%
CTCCAAATCAAACATGCTCT+TGG - chr2_3:49337469-49337488 Msa0296250:intron 40.0%
TGAGTAAAGTTGCCTATGGT+AGG - chr2_3:49337599-49337618 Msa0296250:intron 40.0%
!! AGCATTGTGTCGTTTGAGTA+GGG - chr2_3:49337631-49337650 Msa0296250:intron 40.0%
CATTGGGTGCAGTGTTTAAA+TGG + chr2_3:49337799-49337818 None:intergenic 40.0%
CTCTTCTACCATCTTGCATT+GGG + chr2_3:49337815-49337834 None:intergenic 40.0%
ACTCTTCTACCATCTTGCAT+TGG + chr2_3:49337816-49337835 None:intergenic 40.0%
! GATATTTGTTTCTTTCGGGG+AGG + chr2_3:49337960-49337979 None:intergenic 40.0%
! GGGGATATTTGTTTCTTTCG+GGG + chr2_3:49337963-49337982 None:intergenic 40.0%
AAGGAACATGTCGATAACAG+GGG + chr2_3:49338101-49338120 None:intergenic 40.0%
CAAGGAACATGTCGATAACA+GGG + chr2_3:49338102-49338121 None:intergenic 40.0%
TCAAGGAACATGTCGATAAC+AGG + chr2_3:49338103-49338122 None:intergenic 40.0%
! TGATTCAAGTTCTGTAGCCA+AGG - chr2_3:49338497-49338516 Msa0296250:intron 40.0%
GCTTATTGTACTTTAGGCCT+TGG + chr2_3:49338517-49338536 None:intergenic 40.0%
GACACCGCTTATTGTACTTT+AGG + chr2_3:49338523-49338542 None:intergenic 40.0%
! TGATTCAAGTTCTGTAGCCA+AGG - chr2_3:49338497-49338516 Msa0296250:intron 40.0%
! GATGCTGCATAGTTGCTTTA+TGG - chr2_3:49338679-49338698 Msa0296250:intron 40.0%
!! TTCACACTGTTTCTATCTGC+AGG - chr2_3:49338776-49338795 Msa0296250:intron 40.0%
TACAAGTGCTTACTATCGTG+GGG - chr2_3:49338810-49338829 Msa0296250:intron 40.0%
CAACAATGTTGGAATCTGTG+TGG + chr2_3:49338918-49338937 None:intergenic 40.0%
TAGCCGTATCAACTGAAGAT+GGG - chr2_3:49338977-49338996 Msa0296250:intron 40.0%
! CTTCCCATCTTCAGTTGATA+CGG + chr2_3:49338983-49339002 None:intergenic 40.0%
TGTTAGCAAGAAAGCTGTAG+AGG - chr2_3:49339107-49339126 Msa0296250:intron 40.0%
GTTAGCAAGAAAGCTGTAGA+GGG - chr2_3:49339108-49339127 Msa0296250:intron 40.0%
! TCTATTTTCTCTCCTTTGGC+TGG + chr2_3:49339159-49339178 None:intergenic 40.0%
!! GTTTCAGCTTTGAAGAGAGT+TGG - chr2_3:49339192-49339211 Msa0296250:intron 40.0%
AAGATTGGATTTGCCAACGC+CGG + chr2_3:49335701-49335720 None:intergenic 45.0%
GGTGAAACGAGAGAGAAGAT+TGG + chr2_3:49335716-49335735 None:intergenic 45.0%
AGCGAATTCAACACCAATGG+TGG + chr2_3:49335767-49335786 None:intergenic 45.0%
AACAACAGATCACGATCGAC+GGG + chr2_3:49335921-49335940 None:intergenic 45.0%
GTGTGACATCTATCAGACAC+CGG + chr2_3:49335986-49336005 None:intergenic 45.0%
AGCGTGAGTAAAGTTGCCTA+TGG - chr2_3:49337595-49337614 Msa0296250:intron 45.0%
TAAAGTTGCCTATGGTAGGC+TGG - chr2_3:49337603-49337622 Msa0296250:intron 45.0%
AAAGTTGCCTATGGTAGGCT+GGG - chr2_3:49337604-49337623 Msa0296250:intron 45.0%
!! GAGCATTGTGTCGTTTGAGT+AGG - chr2_3:49337630-49337649 Msa0296250:intron 45.0%
! TGGCACATCAGATTTTGCAC+AGG + chr2_3:49338227-49338246 None:intergenic 45.0%
AACAATTGGGGCACATCAAG+AGG + chr2_3:49338348-49338367 None:intergenic 45.0%
ATGCAGCATCGTGTTCATTC+AGG + chr2_3:49338669-49338688 None:intergenic 45.0%
CGACGTTCTCAAATGTTGCA+TGG + chr2_3:49338870-49338889 None:intergenic 45.0%
CATTTGAGAACGTCGACAGA+TGG - chr2_3:49338875-49338894 Msa0296250:intron 45.0%
TTGATACGGCTACAAGGTGA+CGG + chr2_3:49338969-49338988 None:intergenic 45.0%
! CTTCAGTTGATACGGCTACA+AGG + chr2_3:49338975-49338994 None:intergenic 45.0%
GTAGCCGTATCAACTGAAGA+TGG - chr2_3:49338976-49338995 Msa0296250:intron 45.0%
TTTCATGGAAACCTCAGCTC+TGG - chr2_3:49339029-49339048 Msa0296250:intron 45.0%
GTTGCTGCTCAAGCTAAGAT+TGG - chr2_3:49339214-49339233 Msa0296250:intron 45.0%
ATGTTTCAGTAGGAGCTGCT+GGG - chr2_3:49339383-49339402 Msa0296250:CDS 45.0%
GGGATACTGCTGGTCAAGAA+AGG - chr2_3:49335833-49335852 Msa0296250:three_prime_UTR 50.0%
TTGTCTTCTCTGCCTTGGAC+TGG - chr2_3:49336459-49336478 Msa0296250:intron 50.0%
TGTCTTCTCTGCCTTGGACT+GGG - chr2_3:49336460-49336479 Msa0296250:intron 50.0%
ACTGTTGGGCCGCTTGATAT+TGG - chr2_3:49336946-49336965 Msa0296250:intron 50.0%
AAGTTGCCTATGGTAGGCTG+GGG - chr2_3:49337605-49337624 Msa0296250:intron 50.0%
AGGGTCCCCTCTATTCCAAA+TGG - chr2_3:49337650-49337669 Msa0296250:intron 50.0%
ACACTGCACCCAATGCAAGA+TGG - chr2_3:49337804-49337823 Msa0296250:intron 50.0%
!! TAGTAGGGTGCTGCTAGATG+GGG + chr2_3:49337982-49338001 None:intergenic 50.0%
!! CTAGTAGGGTGCTGCTAGAT+GGG + chr2_3:49337983-49338002 None:intergenic 50.0%
!! ACTAGTAGGGTGCTGCTAGA+TGG + chr2_3:49337984-49338003 None:intergenic 50.0%
GGTATCCCACCCCATAATTC+TGG + chr2_3:49338206-49338225 None:intergenic 50.0%
CAGCATCGTGTTCATTCAGG+TGG + chr2_3:49338666-49338685 None:intergenic 50.0%
!! GCTGTAGAGGGTGCTGAAAA+TGG - chr2_3:49339120-49339139 Msa0296250:intron 50.0%
CATGTTTCAGTAGGAGCTGC+TGG - chr2_3:49339382-49339401 Msa0296250:CDS 50.0%
TGTTTCAGTAGGAGCTGCTG+GGG - chr2_3:49339384-49339403 Msa0296250:CDS 50.0%
! TGTGCACTGTTTTGTGGTCG+TGG - chr2_3:49339516-49339535 Msa0296250:five_prime_UTR 50.0%
!! GTAGTTCTGATCGGCGACTC+CGG - chr2_3:49335679-49335698 Msa0296250:three_prime_UTR 55.0%
! GCTCAGATCTGGGATACTGC+TGG - chr2_3:49335823-49335842 Msa0296250:three_prime_UTR 55.0%
GTCTTCTCTGCCTTGGACTG+GGG - chr2_3:49336461-49336480 Msa0296250:intron 55.0%
TGGGCCGCTTGATATTGGAG+AGG - chr2_3:49336951-49336970 Msa0296250:intron 55.0%
GCTTACTATCGTGGGGCAGT+TGG - chr2_3:49338817-49338836 Msa0296250:intron 55.0%
! CATGGAAACCTCAGCTCTGG+AGG - chr2_3:49339032-49339051 Msa0296250:intron 55.0%
GTTAGTAGCCTCCAGAGCTG+AGG + chr2_3:49339043-49339062 None:intergenic 55.0%
CCTACAGGCTACCGCTAACC+AGG + chr2_3:49336702-49336721 None:intergenic 60.0%
CCTGGTTAGCGGTAGCCTGT+AGG - chr2_3:49336699-49336718 Msa0296250:intron 60.0%
TTGCCTATGGTAGGCTGGGG+TGG - chr2_3:49337608-49337627 Msa0296250:intron 60.0%
AACGCGTCTGTGCCAGCCAA+AGG - chr2_3:49339144-49339163 Msa0296250:intron 60.0%
ACCGACACGGAAGACGCGTC+CGG - chr2_3:49335964-49335983 Msa0296250:CDS 65.0%
ACCGGACGCGTCTTCCGTGT+CGG + chr2_3:49335968-49335987 None:intergenic 65.0%
AGGGATGGTGCCCCAGTCCA+AGG + chr2_3:49336474-49336493 None:intergenic 65.0%
GCTCCACCCCAGCCTACCAT+AGG + chr2_3:49337614-49337633 None:intergenic 65.0%
CTGATCGGCGACTCCGGCGT+TGG - chr2_3:49335685-49335704 Msa0296250:three_prime_UTR 70.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 49335515 49339572 49335515 ID=Msa0296250;Name=Msa0296250
chr2_3 mRNA 49335515 49339572 49335515 ID=Msa0296250-mRNA-1;Parent=Msa0296250;Name=Msa0296250-mRNA-1;_AED=0.08;_eAED=0.08;_QI=119|1|1|1|1|1|2|342|217
chr2_3 exon 49335515 49336289 49335515 ID=Msa0296250-mRNA-1:exon:15539;Parent=Msa0296250-mRNA-1
chr2_3 exon 49339233 49339572 49339233 ID=Msa0296250-mRNA-1:exon:15538;Parent=Msa0296250-mRNA-1
chr2_3 five_prime_UTR 49339454 49339572 49339454 ID=Msa0296250-mRNA-1:five_prime_utr;Parent=Msa0296250-mRNA-1
chr2_3 CDS 49339233 49339453 49339233 ID=Msa0296250-mRNA-1:cds;Parent=Msa0296250-mRNA-1
chr2_3 CDS 49335857 49336289 49335857 ID=Msa0296250-mRNA-1:cds;Parent=Msa0296250-mRNA-1
chr2_3 three_prime_UTR 49335515 49335856 49335515 ID=Msa0296250-mRNA-1:three_prime_utr;Parent=Msa0296250-mRNA-1
Gene Sequence

>Msa0296250

ATGGCGGGTTACAGAGCAGACGACGACTATGACTACCTATTCAAGGTAGTTCTGATCGGCGACTCCGGCGTTGGCAAATCCAATCTTCTCTCTCGTTTCACCAAAAACGAGTTCAATCTTGAATCCAAATCCACCATTGGTGTTGAATTCGCTACTCGTACTTTAAATGTTGATTCTAAAGTTATTAAGGCTCAGATCTGGGATACTGCTGGTCAAGAAAGGTATCGCGCCATTACAAGTGCTTACTATCGTGGGGCAGTTGGTGCACTTCTTGTATATGATGTTACACGCCATGCAACATTTGAGAACGTCGACAGATGGCTGAAAGAACTGCGAAACCACACAGATTCCAACATTGTTGTGATGCTTGTTGGCAACAAAGCAGATCTCCGTCACCTTGTAGCCGTATCAACTGAAGATGGGAAGTCTTATGCCGAGAAAGAATCACTTTATTTCATGGAAACCTCAGCTCTGGAGGCTACTAACGTAGAGAATTCATTTGCTGAAGTTCTGACTCAAATCTACCGTATTGTTAGCAAGAAAGCTGTAGAGGGTGCTGAAAATGGAAACGCGTCTGTGCCAGCCAAAGGAGAGAAAATAGATCTTAAGAATGATGTTTCAGCTTTGAAGAGAGTTGGTTGCTGCTCAAGCTAA
Protein sequence

>Msa0296250

MAGYRADDDYDYLFKVVLIGDSGVGKSNLLSRFTKNEFNLESKSTIGVEFATRTLNVDSKVIKAQIWDTAGQERYRAITSAYYRGAVGALLVYDVTRHATFENVDRWLKELRNHTDSNIVVMLVGNKADLRHLVAVSTEDGKSYAEKESLYFMETSALEATNVENSFAEVLTQIYRIVSKKAVEGAENGNASVPAKGEKIDLKNDVSALKRVGCCSS*