AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0278520

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0278520 MtrunA17_Chr2g0291581 91.207 671 43 2 60 730 33 687 0.0 1263
Msa0278520 MtrunA17_Chr2g0291591 49.822 562 273 3 38 596 1 556 0.0 567
Msa0278520 MtrunA17_Chr2g0296781 37.790 561 339 8 111 665 1 557 2.18e-125 390
Msa0278520 MtrunA17_Chr5g0441581 35.453 629 367 9 107 721 32 635 2.01e-121 379
Msa0278520 MtrunA17_Chr1g0207551 35.397 630 382 11 99 719 27 640 1.22e-117 372
Msa0278520 MtrunA17_Chr2g0329031 34.961 635 359 12 107 721 14 614 7.61e-117 368
Msa0278520 MtrunA17_Chr6g0477261 40.090 444 262 4 224 665 5 446 1.52e-111 352
Msa0278520 MtrunA17_Chr1g0196541 36.607 560 347 8 111 665 1 557 1.63e-111 354
Msa0278520 MtrunA17_Chr3g0080351 33.861 632 368 8 105 718 74 673 1.86e-109 352
Msa0278520 MtrunA17_Chr1g0202341 32.031 640 402 11 105 726 49 673 4.76e-109 346
Msa0278520 MtrunA17_Chr5g0448761 32.348 643 408 14 105 729 58 691 1.51e-99 324
Msa0278520 MtrunA17_Chr2g0287061 31.442 617 376 11 105 718 181 753 2.84e-90 300
Msa0278520 MtrunA17_Chr2g0287061 34.694 98 55 1 107 204 29 117 5.99e-11 66.2
Msa0278520 MtrunA17_Chr5g0440451 28.963 656 419 14 92 722 28 661 2.56e-88 297
Msa0278520 MtrunA17_Chr5g0448511 32.533 458 290 6 272 722 11 456 6.53e-80 265
Msa0278520 MtrunA17_Chr3g0118611 31.858 452 295 6 273 722 118 558 2.31e-78 267
Msa0278520 MtrunA17_Chr8g0363841 28.205 585 405 7 109 684 19 597 2.65e-70 243
Msa0278520 MtrunA17_Chr2g0324011 27.907 602 400 13 107 692 10 593 2.85e-66 230
Msa0278520 MtrunA17_Chr1g0203721 27.202 647 417 18 108 725 64 685 4.07e-62 223
Msa0278520 MtrunA17_Chr2g0287651 26.609 575 380 14 111 664 79 632 4.29e-57 209
Msa0278520 MtrunA17_Chr6g0458391 25.744 571 381 11 107 664 225 765 5.53e-56 204
Msa0278520 MtrunA17_Chr3g0082121 26.498 634 413 14 107 718 43 645 1.20e-55 202
Msa0278520 MtrunA17_Chr1g0198761 27.332 611 388 17 80 662 28 610 2.30e-55 202
Msa0278520 MtrunA17_Chr1g0203051 26.239 545 371 13 120 651 95 621 1.92e-54 198
Msa0278520 MtrunA17_Chr2g0276841 26.655 589 378 18 107 669 70 630 1.16e-53 197
Msa0278520 MtrunA17_Chr2g0290391 25.543 599 393 15 103 672 47 621 1.48e-42 164
Msa0278520 MtrunA17_Chr2g0294841 23.576 632 387 13 79 659 17 603 3.63e-39 155
Msa0278520 MtrunA17_Chr5g0431001 25.858 437 290 11 95 507 54 480 7.40e-36 142
Msa0278520 MtrunA17_Chr1g0164591 26.774 310 207 5 190 491 11 308 9.42e-31 123
Msa0278520 MtrunA17_Chr7g0258861 27.915 283 184 5 107 374 10 287 5.40e-28 115
Msa0278520 MtrunA17_Chr2g0291601 86.792 53 7 0 180 232 1 53 1.40e-25 99.8
Msa0278520 MtrunA17_Chr2g0308841 24.454 229 163 6 435 661 4 224 1.24e-18 86.7
Msa0278520 MtrunA17_Chr4g0033611 40.196 102 56 2 107 204 76 176 2.31e-14 73.9
Msa0278520 MtrunA17_Chr2g0329361 33.962 106 64 1 105 204 59 164 1.99e-13 70.9
Msa0278520 MtrunA17_Chr2g0329421 38.235 102 58 2 107 204 27 127 7.55e-13 68.6
Msa0278520 MtrunA17_Chr1g0172041 35.165 91 57 2 575 665 1 89 4.23e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0278520 AT1G52520.1 60.901 688 253 10 50 730 25 703 0.0 861
Msa0278520 AT1G80010.1 45.510 657 331 12 90 728 76 723 0.0 567
Msa0278520 AT3G22170.2 32.959 713 398 13 37 719 6 668 1.80e-121 384
Msa0278520 AT3G22170.1 32.959 713 398 13 37 719 6 668 1.80e-121 384
Msa0278520 AT4G15090.1 33.589 652 406 9 86 725 30 666 2.62e-121 383
Msa0278520 AT4G15090.5 33.589 652 406 9 86 725 30 666 2.62e-121 383
Msa0278520 AT4G15090.3 33.589 652 406 9 86 725 30 666 2.62e-121 383
Msa0278520 AT4G15090.2 33.589 652 406 9 86 725 30 666 2.62e-121 383
Msa0278520 AT4G15090.4 33.589 652 406 9 86 725 30 666 2.62e-121 383
Msa0278520 AT1G76320.3 35.397 630 355 13 111 721 1 597 1.85e-110 350
Msa0278520 AT1G76320.2 35.238 630 356 12 111 721 1 597 4.18e-110 351
Msa0278520 AT1G76320.1 35.238 630 356 12 111 721 1 597 5.21e-110 350
Msa0278520 AT1G76320.4 35.238 630 356 12 111 721 1 597 5.21e-110 350
Msa0278520 AT4G19990.3 34.429 578 314 10 110 664 24 559 2.68e-95 312
Msa0278520 AT4G19990.4 34.429 578 314 10 110 664 24 559 2.68e-95 312
Msa0278520 AT4G19990.5 34.429 578 314 10 110 664 24 559 3.33e-95 312
Msa0278520 AT4G19990.7 34.429 578 314 10 110 664 38 573 4.45e-95 312
Msa0278520 AT4G19990.6 34.429 578 314 10 110 664 24 559 5.53e-95 312
Msa0278520 AT4G19990.2 34.429 578 314 10 110 664 24 559 5.53e-95 312
Msa0278520 AT4G38180.1 31.329 632 411 12 105 722 71 693 1.56e-92 306
Msa0278520 AT2G27110.2 30.047 639 407 14 93 728 40 641 3.94e-91 303
Msa0278520 AT2G27110.1 30.047 639 407 14 93 728 40 641 3.94e-91 303
Msa0278520 AT4G19990.1 35.400 500 277 7 170 664 44 502 1.36e-88 293
Msa0278520 AT3G06250.2 30.255 628 389 13 93 718 176 756 1.76e-88 294
Msa0278520 AT3G06250.2 32.710 107 63 1 105 211 26 123 2.39e-12 70.9
Msa0278520 AT3G06250.1 30.255 628 389 13 93 718 176 756 1.76e-88 294
Msa0278520 AT3G06250.1 32.710 107 63 1 105 211 26 123 2.39e-12 70.9
Msa0278520 AT3G06250.3 30.255 628 389 13 93 718 176 756 1.76e-88 294
Msa0278520 AT3G06250.3 32.710 107 63 1 105 211 26 123 2.39e-12 70.9
Msa0278520 AT5G18960.2 30.782 614 379 15 107 718 211 780 2.48e-86 289
Msa0278520 AT5G18960.1 30.782 614 379 15 107 718 211 780 2.48e-86 289
Msa0278520 AT2G27110.3 31.809 481 316 7 249 728 27 496 2.26e-83 279
Msa0278520 AT2G32250.8 34.234 444 265 9 284 719 139 563 4.27e-73 253
Msa0278520 AT2G32250.5 34.234 444 265 9 284 719 180 604 4.52e-73 253
Msa0278520 AT2G32250.7 34.234 444 265 9 284 719 139 563 5.04e-73 253
Msa0278520 AT2G32250.6 34.234 444 265 9 284 719 180 604 9.36e-73 253
Msa0278520 AT2G32250.1 34.234 444 265 9 284 719 180 604 9.36e-73 253
Msa0278520 AT2G32250.3 34.234 444 265 9 284 719 180 604 9.36e-73 253
Msa0278520 AT2G32250.2 34.234 444 265 9 284 719 180 604 1.14e-72 252
Msa0278520 AT2G32250.4 34.234 444 265 9 284 719 180 604 1.14e-72 252
Msa0278520 AT4G38170.1 31.920 448 291 7 277 722 21 456 1.72e-72 246
Msa0278520 AT1G10240.2 26.261 575 386 14 107 661 48 604 3.25e-52 193
Msa0278520 AT1G10240.1 26.261 575 386 14 107 661 48 604 3.25e-52 193
Msa0278520 AT5G28530.1 24.613 646 438 16 106 725 54 676 3.15e-39 155
Msa0278520 AT5G28530.3 24.730 647 432 17 106 725 54 672 8.65e-39 154
Msa0278520 AT5G28530.2 24.730 647 432 17 106 725 54 672 8.65e-39 154
Msa0278520 AT5G28530.4 23.307 635 397 19 106 725 54 613 8.69e-28 119
Msa0278520 AT5G28530.5 23.622 635 391 20 106 725 54 609 1.28e-27 119
Msa0278520 AT3G59470.3 36.607 112 66 2 96 204 61 170 4.23e-14 73.2
Msa0278520 AT3G59470.1 36.607 112 66 2 96 204 61 170 4.23e-14 73.2
Msa0278520 AT3G59470.2 36.607 112 66 2 96 204 73 182 4.99e-14 73.2

Find 135 sgRNAs with CRISPR-Local

Find 6 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTCAACTTATTAGAATATT+TGG 0.117558 2_3:-11504604 None:intergenic
AGTGACAAGTCTTTATAATT+TGG 0.149627 2_3:-11502782 None:intergenic
TTATCTTGACAACACTTATT+TGG 0.170954 2_3:+11504794 Msa0278520:CDS
GAAGAAATGTTCTCTTGTTT+TGG 0.204791 2_3:+11505536 Msa0278520:CDS
TCAATGATGAAGGGCATTTA+AGG 0.212565 2_3:+11504712 Msa0278520:CDS
GGCATTTAAGGAATGCATTA+TGG 0.227561 2_3:+11504724 Msa0278520:CDS
CATGGTCAATCAGTGTTATT+AGG 0.249432 2_3:+11504864 Msa0278520:CDS
GGATTCATGATTCAATGTTT+TGG 0.288413 2_3:+11505179 Msa0278520:CDS
AAATAATAGAATCATATAAA+TGG 0.289585 2_3:+11504907 Msa0278520:CDS
TTAGGTTGTGGCCTGCTTGC+AGG 0.291307 2_3:+11504882 Msa0278520:CDS
TCCATCAAATAAAAGAAGTT+TGG 0.301368 2_3:-11504687 None:intergenic
CGATATTTCTGAGGGTAAAA+AGG 0.306656 2_3:+11504107 Msa0278520:CDS
GGTATGTCTGCAACACGGTC+TGG 0.311372 2_3:+11505287 Msa0278520:CDS
CTGCAGTTCAAAAGAGCATT+TGG 0.324605 2_3:-11505462 None:intergenic
GGGAAACAAACGGGAAATTA+AGG 0.326033 2_3:+11505625 Msa0278520:CDS
TGGCCCTATCACCGTCTTCT+TGG 0.333592 2_3:+11505580 Msa0278520:CDS
CAAAATTACAAAACTGATAC+TGG 0.344469 2_3:+11501540 None:intergenic
TTGACCAAGAAGACGGTGAT+AGG 0.355516 2_3:-11505584 None:intergenic
TCATTACAAGGTAGCGTTGC+AGG 0.357719 2_3:+11505940 Msa0278520:CDS
GCTAAAGCAATGATGAGATC+TGG 0.357977 2_3:-11505030 None:intergenic
CTCGAACATAACCACATCTT+AGG 0.364101 2_3:+11504414 Msa0278520:CDS
CATGGCAGGACAACCAGTTC+TGG 0.364832 2_3:-11504340 None:intergenic
ATCCCTCTTGTGGCTTTAGT+TGG 0.368401 2_3:+11504831 Msa0278520:CDS
GCATTTAAGGAATGCATTAT+GGG 0.369143 2_3:+11504725 Msa0278520:CDS
TGCAGAGGGGAAAACAATTA+AGG 0.370228 2_3:+11504506 Msa0278520:CDS
ACGGCAGAGGAAATTATAAA+TGG 0.374105 2_3:-11504649 None:intergenic
CACACAAAATCCAACCTCTT+TGG 0.375093 2_3:-11504202 None:intergenic
TATGGGTTGATGCTAAATCT+AGG 0.378405 2_3:+11504742 Msa0278520:CDS
GTGTGAGGATTAAGAATTCA+TGG 0.378670 2_3:+11504220 Msa0278520:CDS
GTATGTCTGCAACACGGTCT+GGG 0.384016 2_3:+11505288 Msa0278520:CDS
ACATGGAACACATGTATATC+AGG 0.387568 2_3:+11504939 Msa0278520:CDS
TTTACGAAGACCGAGTTCAT+TGG 0.400562 2_3:+11505231 Msa0278520:CDS
AACCGATTAGACCATTGAAT+AGG 0.415320 2_3:-11505869 None:intergenic
ACCCTATTCAATGGTCTAAT+CGG 0.415941 2_3:+11505867 Msa0278520:CDS
CCACAAACAGACTTCTTTGA+AGG 0.418328 2_3:+11505343 Msa0278520:CDS
TTCCACTTCAAGTTGGAACT+TGG 0.419493 2_3:-11505516 None:intergenic
AGAGCACCATCTTCATCTCT+TGG 0.423726 2_3:-11504063 None:intergenic
ACCGATTAGACCATTGAATA+GGG 0.424191 2_3:-11505868 None:intergenic
CCTTCAAAGAAGTCTGTTTG+TGG 0.428381 2_3:-11505343 None:intergenic
TTGATGGATTTCAATGATGA+AGG 0.428882 2_3:+11504702 Msa0278520:CDS
ATTTCAATGCATTCTCCATC+CGG 0.439531 2_3:-11504039 None:intergenic
AGATGAAGAACGATATTTAA+CGG 0.441556 2_3:+11501664 Msa0278520:CDS
TCTTCATCTTCTTCTTCTGA+AGG 0.442963 2_3:-11501650 None:intergenic
TCCCTCCTCCACAACTTGTA+AGG 0.455897 2_3:-11505903 None:intergenic
CAATCAGTGTTATTAGGTTG+TGG 0.457165 2_3:+11504870 Msa0278520:CDS
TTACGAAGACCGAGTTCATT+GGG 0.459257 2_3:+11505232 Msa0278520:CDS
CATGATAAGGATTAAGTTAG+TGG 0.463158 2_3:+11504359 Msa0278520:CDS
TCTTCTTCTTCTGAAGGGAG+AGG 0.466438 2_3:-11501644 None:intergenic
TTCCCCTCTGCATCAGAACT+AGG 0.470411 2_3:-11504495 None:intergenic
TTTGATCGAGGGAAACAAAC+GGG 0.479414 2_3:+11505616 Msa0278520:CDS
CCCTCTGATCGGATCATGAC+GGG 0.480660 2_3:+11502743 Msa0278520:CDS
ATTTGCTATGCCAAAGAGGT+TGG 0.481359 2_3:+11504192 Msa0278520:CDS
TCGTGATGTAGAACACAAAC+AGG 0.481522 2_3:+11505988 Msa0278520:CDS
TTGAGAAAAGAAACCAGAAC+TGG 0.483081 2_3:+11504327 Msa0278520:CDS
CTGGTTGTCCTGCCATGATA+AGG 0.483669 2_3:+11504346 Msa0278520:CDS
CTTCATCTTCTTCTTCTGAA+GGG 0.486139 2_3:-11501649 None:intergenic
TAGAAGTGCCTTACAAGTTG+TGG 0.488022 2_3:+11505895 Msa0278520:CDS
GTGCTTTGTTGTAGCAGTCA+AGG 0.493576 2_3:+11504276 Msa0278520:CDS
AGCTCTGTCATCTCTTGCAT+TGG 0.494419 2_3:-11504577 None:intergenic
CTAAAGCAATGATGAGATCT+GGG 0.494575 2_3:-11505029 None:intergenic
CACCAAGTTCCAACTTGAAG+TGG 0.497024 2_3:+11505514 Msa0278520:CDS
CTCCTAAGTACATTCTTTCG+CGG 0.499725 2_3:+11505774 Msa0278520:CDS
GGAGGAGGGAGTAATTTCTC+TGG 0.511124 2_3:+11505916 Msa0278520:CDS
AGGACTAAAACGGTTGTGAC+CGG 0.512191 2_3:-11505828 None:intergenic
CTATTATTTCACCTGCAAGC+AGG 0.514073 2_3:-11504893 None:intergenic
AACTTGTCCTTCAGATATAC+CGG 0.514192 2_3:-11505257 None:intergenic
TGCCTTACAAGTTGTGGAGG+AGG 0.514813 2_3:+11505901 Msa0278520:CDS
AATAAGTTGAACCTAAGAAA+AGG 0.515686 2_3:+11504615 Msa0278520:CDS
ACATTTCTTCCACTTCAAGT+TGG 0.516480 2_3:-11505523 None:intergenic
AACTTAATCCTTATCATGGC+AGG 0.517948 2_3:-11504354 None:intergenic
TGATGGATTTCAATGATGAA+GGG 0.520805 2_3:+11504703 Msa0278520:CDS
CAGTATCATCAGGACTAAAA+CGG 0.522367 2_3:-11505838 None:intergenic
GGGTCAGTATCAGTATCATC+AGG 0.522563 2_3:-11505848 None:intergenic
AATTTCTCTGGATCATTACA+AGG 0.524035 2_3:+11505928 Msa0278520:CDS
CTGATACTGACCCTATTCAA+TGG 0.530519 2_3:+11505858 Msa0278520:CDS
CTATCTGTAATAATAGTTTG+AGG 0.532529 2_3:-11504969 None:intergenic
TCAAACTCCATTCCGACGGC+AGG 0.533529 2_3:-11504141 None:intergenic
CACTAACTTAATCCTTATCA+TGG 0.537066 2_3:-11504358 None:intergenic
TAGTCAATTGCATACGGCAG+AGG 0.541377 2_3:-11504662 None:intergenic
TTGGATTAGTCAATTGCATA+CGG 0.547484 2_3:-11504668 None:intergenic
GATTGAATTCGATGCAGCAT+GGG 0.556013 2_3:+11505157 Msa0278520:CDS
ATGGGTTGATGCTAAATCTA+GGG 0.557484 2_3:+11504743 Msa0278520:CDS
TGATTGAATTCGATGCAGCA+TGG 0.558452 2_3:+11505156 Msa0278520:CDS
TGTTGCACCTGCCGTCGGAA+TGG 0.558619 2_3:+11504134 Msa0278520:CDS
TGACCAAGAAGACGGTGATA+GGG 0.563728 2_3:-11505583 None:intergenic
ACAATGCAATTACACGTGGA+TGG 0.565499 2_3:+11505560 Msa0278520:CDS
CCCGTCATGATCCGATCAGA+GGG 0.567046 2_3:-11502743 None:intergenic
TGCAGCAAGCAATCGCCGGA+TGG 0.569478 2_3:+11504024 Msa0278520:CDS
GAATTTGTTGCACCTGCCGT+CGG 0.571617 2_3:+11504129 Msa0278520:CDS
GCCTTACAAGTTGTGGAGGA+GGG 0.572299 2_3:+11505902 Msa0278520:CDS
TTATATTTGCTATGCCAAAG+AGG 0.572634 2_3:+11504188 Msa0278520:CDS
AGTTAGTGGAGTCTCAGAGA+TGG 0.573080 2_3:+11504373 Msa0278520:CDS
AGCTCTTTACAAGAAGTACA+AGG 0.577775 2_3:+11505388 Msa0278520:CDS
CAAATATGAAATCCCTCTTG+TGG 0.578562 2_3:+11504821 Msa0278520:CDS
TATCACCGTCTTCTTGGTCA+AGG 0.580733 2_3:+11505586 Msa0278520:CDS
GGACTAAAACGGTTGTGACC+GGG 0.581921 2_3:-11505827 None:intergenic
ACCATGCAGCAAGCAATCGC+CGG 0.584825 2_3:+11504020 Msa0278520:CDS
TTGTCAAGACGATATTTCTG+AGG 0.585249 2_3:+11504098 Msa0278520:CDS
TAGTGGAGTCTCAGAGATGG+AGG 0.590281 2_3:+11504376 Msa0278520:CDS
TTGCCTAGTTCTGATGCAGA+GGG 0.592446 2_3:+11504492 Msa0278520:CDS
ATACCGGTGCCCAATGAACT+CGG 0.592551 2_3:-11505241 None:intergenic
GAAATTCCAAGAGATGAAGA+TGG 0.592964 2_3:+11504057 Msa0278520:CDS
CTAAGTACATTCTTTCGCGG+TGG 0.595177 2_3:+11505777 Msa0278520:CDS
GTATCTTAGCACCTAAGATG+TGG 0.595734 2_3:-11504425 None:intergenic
ACCCGTCATGATCCGATCAG+AGG 0.597109 2_3:-11502744 None:intergenic
CGCGAAAGAATGTACTTAGG+AGG 0.597604 2_3:-11505773 None:intergenic
ATAAATGGTTGTTTAGAACA+TGG 0.600697 2_3:+11504922 Msa0278520:CDS
TCCGGCGATTGCTTGCTGCA+TGG 0.603156 2_3:-11504021 None:intergenic
GTTCATGTGAACCCTCTGAT+CGG 0.604605 2_3:+11502732 Msa0278520:CDS
ATTCCAACTAAAGCCACAAG+AGG 0.609409 2_3:-11504834 None:intergenic
AAGTGCCTTACAAGTTGTGG+AGG 0.614403 2_3:+11505898 Msa0278520:CDS
TTGGGCACCGGTATATCTGA+AGG 0.614520 2_3:+11505250 Msa0278520:CDS
TGTCAAGACGATATTTCTGA+GGG 0.614817 2_3:+11504099 Msa0278520:CDS
TTCCAACTAAAGCCACAAGA+GGG 0.616806 2_3:-11504833 None:intergenic
TATGTCTGCAACACGGTCTG+GGG 0.623170 2_3:+11505289 Msa0278520:CDS
TTCTGAAGGGAGAGGCTCAA+CGG 0.623564 2_3:-11501636 None:intergenic
TTGCTGGTATGTCTGCAACA+CGG 0.624394 2_3:+11505282 Msa0278520:CDS
ACCCTCTGATCGGATCATGA+CGG 0.625114 2_3:+11502742 Msa0278520:CDS
GAGCTCTAATCTCGATGTCA+CGG 0.627743 2_3:+11502805 Msa0278520:CDS
GTTGCCTAGTTCTGATGCAG+AGG 0.630104 2_3:+11504491 Msa0278520:CDS
CTAATAACACTGATTGACCA+TGG 0.632937 2_3:-11504863 None:intergenic
AGACCGAGTTCATTGGGCAC+CGG 0.643751 2_3:+11505238 Msa0278520:CDS
ATTGAATTCGATGCAGCATG+GGG 0.645271 2_3:+11505158 Msa0278520:CDS
CACCGCGAAAGAATGTACTT+AGG 0.647338 2_3:-11505776 None:intergenic
TGGGACAATGCAATTACACG+TGG 0.650233 2_3:+11505556 Msa0278520:CDS
TTACAAAACTGATACTGGCG+CGG 0.654812 2_3:+11501545 None:intergenic
GCGCTCCTTGACCAAGAAGA+CGG 0.655876 2_3:-11505591 None:intergenic
TGATTCAAACTCCATTCCGA+CGG 0.656357 2_3:-11504145 None:intergenic
TTGGAGTTAATGATAATGAG+TGG 0.659577 2_3:+11505198 Msa0278520:CDS
GCACTTGTGATAGATACAGA+AGG 0.672329 2_3:+11504537 Msa0278520:CDS
TGAAGGGAGAGGCTCAACGG+CGG 0.678410 2_3:-11501633 None:intergenic
TTAGTTGGAATAAATCACCA+TGG 0.700334 2_3:+11504846 Msa0278520:CDS
TCAGAGATGGAGGATATGCG+AGG 0.712448 2_3:+11504386 Msa0278520:CDS
TGCCTAGTTCTGATGCAGAG+GGG 0.720033 2_3:+11504493 Msa0278520:CDS
GCTAAATCTAGGGCTGCATG+CGG 0.722557 2_3:+11504753 Msa0278520:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATGTTTTGATGTCGAATCTT+GGG + chr2_3:11501591-11501610 Msa0278520:exon 30.0%
AATTTCTCTGGATCATTACA+AGG + chr2_3:11505928-11505947 Msa0278520:CDS 30.0%
!! TTTTGAAGAATCACTTCATC+AGG + chr2_3:11505964-11505983 Msa0278520:CDS 30.0%
!!! CATGTTTTGATGTCGAATCT+TGG + chr2_3:11501590-11501609 Msa0278520:exon 35.0%
TCGTGATGTAGAACACAAAC+AGG + chr2_3:11505988-11506007 Msa0278520:CDS 40.0%
TCATTACAAGGTAGCGTTGC+AGG + chr2_3:11505940-11505959 Msa0278520:CDS 45.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 11501548 11506015 11501548 ID=Msa0278520;Name=Msa0278520
chr2_3 mRNA 11501548 11506015 11501548 ID=Msa0278520-mRNA-1;Parent=Msa0278520;Name=Msa0278520-mRNA-1;_AED=0.20;_eAED=0.20;_QI=51|1|0.66|1|0.5|1|3|0|730
chr2_3 exon 11501548 11501685 11501548 ID=Msa0278520-mRNA-1:exon:5224;Parent=Msa0278520-mRNA-1
chr2_3 exon 11502731 11502826 11502731 ID=Msa0278520-mRNA-1:exon:5225;Parent=Msa0278520-mRNA-1
chr2_3 exon 11504006 11506015 11504006 ID=Msa0278520-mRNA-1:exon:5226;Parent=Msa0278520-mRNA-1
chr2_3 five_prime_UTR 11501548 11501598 11501548 ID=Msa0278520-mRNA-1:five_prime_utr;Parent=Msa0278520-mRNA-1
chr2_3 CDS 11501599 11501685 11501599 ID=Msa0278520-mRNA-1:cds;Parent=Msa0278520-mRNA-1
chr2_3 CDS 11502731 11502826 11502731 ID=Msa0278520-mRNA-1:cds;Parent=Msa0278520-mRNA-1
chr2_3 CDS 11504006 11506015 11504006 ID=Msa0278520-mRNA-1:cds;Parent=Msa0278520-mRNA-1
Gene Sequence

>Msa0278520

ATGTCGAATCTTGGGACTGAACAACAACTTGTCTCCGCCGTTGAGCCTCTCCCTTCAGAAGAAGAAGATGAAGAACGATATTTAACGAGTTCATGTGAACCCTCTGATCGGATCATGACGGGTGATACTGTGTGTGCTCCAAATTATAAAGACTTGTCACTGAGCTCTAATCTCGATGTCACGATGGAAGATACTTCACCATGCAGCAAGCAATCGCCGGATGGAGAATGCATTGAAATTCCAAGAGATGAAGATGGTGCTCTAATTGAATTTGATTGTCAAGACGATATTTCTGAGGGTAAAAAGGAATTTGTTGCACCTGCCGTCGGAATGGAGTTTGAATCATATGATGATGCTTACAATTATTATATTTGCTATGCCAAAGAGGTTGGATTTTGTGTGAGGATTAAGAATTCATGGTTCAAGCGAAATAGCAAAGAAAAATACGGTGCAGTGCTTTGTTGTAGCAGTCAAGGTTTCAAAAGAATAAAAGATGTTAACAATTTGAGAAAAGAAACCAGAACTGGTTGTCCTGCCATGATAAGGATTAAGTTAGTGGAGTCTCAGAGATGGAGGATATGCGAGGTTACTCTCGAACATAACCACATCTTAGGTGCTAAGATACATAAATCCATTAAAAAGATGGGAAGTGGAACTAAAAATAACTCGTTGCCTAGTTCTGATGCAGAGGGGAAAACAATTAAGGTATATCATGCACTTGTGATAGATACAGAAGGTAATGACAATTTAAACTCCAATGCAAGAGATGACAGAGCTTTCTCCAAATATTCTAATAAGTTGAACCTAAGAAAAGGTGACACACAAGCCATTTATAATTTCCTCTGCCGTATGCAATTGACTAATCCAAACTTCTTTTATTTGATGGATTTCAATGATGAAGGGCATTTAAGGAATGCATTATGGGTTGATGCTAAATCTAGGGCTGCATGCGGTTATTTTAGTGATGTCATTTATCTTGACAACACTTATTTGGTAAACAAATATGAAATCCCTCTTGTGGCTTTAGTTGGAATAAATCACCATGGTCAATCAGTGTTATTAGGTTGTGGCCTGCTTGCAGGTGAAATAATAGAATCATATAAATGGTTGTTTAGAACATGGAACACATGTATATCAGGTTGTTCTCCTCAAACTATTATTACAGATAGATGCAAAGCTTTGCAGAGTGTAATTGCTGAAGTTTTTCCCAGATCTCATCATTGCTTTAGCTTGTCATTAATTATGAAAAAAGTACCAGAAAAACTGGGAGGGTTGCACAATTATGATGCAATCAAGAAAGCGCTGATTAAAGCAGTTTATGAGACATCAAAAGTGATTGAATTCGATGCAGCATGGGGATTCATGATTCAATGTTTTGGAGTTAATGATAATGAGTGGCTTTGTTCACTTTACGAAGACCGAGTTCATTGGGCACCGGTATATCTGAAGGACAAGTTTTTTGCTGGTATGTCTGCAACACGGTCTGGGGAAAGCATTAGTCCTTTTTTCGATAAATATGTCCACAAACAGACTTCTTTGAAGGAGTTTCTTGACAAGTATGAATTAGCTCTTTACAAGAAGTACAAGGAAGAATCTCTTGCAGATATTGAATCGAGAAGCTCAAGCCCTTTGCTAAAAACCAAATGCTCTTTTGAACTGCAGCTCTCTAGTATGTACACAAGAGAATTATTCACCAAGTTCCAACTTGAAGTGGAAGAAATGTTCTCTTGTTTTGGGACAATGCAATTACACGTGGATGGCCCTATCACCGTCTTCTTGGTCAAGGAGCGCGTTTTGATCGAGGGAAACAAACGGGAAATTAAGGATTTTGAAGTTGTGTATAGTAAAACATCAGTTGAAGTTCGTTGTATCTGTTGTTGCTTTAATTTTTATGGATATCTTTGTCGGCATGCGTTGTGTGTACTCAATTTTATTGGGGTGGAGGAGATTCCTCCTAAGTACATTCTTTCGCGGTGGAATAAAGATTACAAGCGCTTTTGTATTCCCGGTCACAACCGTTTTAGTCCTGATGATACTGATACTGACCCTATTCAATGGTCTAATCGGTTATTTAGAAGTGCCTTACAAGTTGTGGAGGAGGGAGTAATTTCTCTGGATCATTACAAGGTAGCGTTGCAGGCTTTTGAAGAATCACTTCATCAGGTTCGTGATGTAGAACACAAACAGGAGTAA
Protein sequence

>Msa0278520

MSNLGTEQQLVSAVEPLPSEEEDEERYLTSSCEPSDRIMTGDTVCAPNYKDLSLSSNLDVTMEDTSPCSKQSPDGECIEIPRDEDGALIEFDCQDDISEGKKEFVAPAVGMEFESYDDAYNYYICYAKEVGFCVRIKNSWFKRNSKEKYGAVLCCSSQGFKRIKDVNNLRKETRTGCPAMIRIKLVESQRWRICEVTLEHNHILGAKIHKSIKKMGSGTKNNSLPSSDAEGKTIKVYHALVIDTEGNDNLNSNARDDRAFSKYSNKLNLRKGDTQAIYNFLCRMQLTNPNFFYLMDFNDEGHLRNALWVDAKSRAACGYFSDVIYLDNTYLVNKYEIPLVALVGINHHGQSVLLGCGLLAGEIIESYKWLFRTWNTCISGCSPQTIITDRCKALQSVIAEVFPRSHHCFSLSLIMKKVPEKLGGLHNYDAIKKALIKAVYETSKVIEFDAAWGFMIQCFGVNDNEWLCSLYEDRVHWAPVYLKDKFFAGMSATRSGESISPFFDKYVHKQTSLKEFLDKYELALYKKYKEESLADIESRSSSPLLKTKCSFELQLSSMYTRELFTKFQLEVEEMFSCFGTMQLHVDGPITVFLVKERVLIEGNKREIKDFEVVYSKTSVEVRCICCCFNFYGYLCRHALCVLNFIGVEEIPPKYILSRWNKDYKRFCIPGHNRFSPDDTDTDPIQWSNRLFRSALQVVEEGVISLDHYKVALQAFEESLHQVRDVEHKQE*