AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1007230


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1007230 MtrunA17_Chr7g0217621 90.099 202 18 1 1 200 1 202 5.84e-120 338
Msa1007230 MtrunA17_Chr5g0408251 79.902 204 33 2 1 200 1 200 9.26e-109 310
Msa1007230 MtrunA17_Chr4g0071941 66.434 143 44 2 62 200 8 150 2.13e-52 165
Msa1007230 MtrunA17_Chr4g0042491 48.148 108 53 2 89 196 72 176 6.33e-21 85.5
Msa1007230 MtrunA17_Chr7g0217611 83.721 43 7 0 96 138 1 43 6.67e-17 71.2
Msa1007230 MtrunA17_Chr3g0139311 39.167 120 64 2 84 195 60 178 7.95e-13 63.9
Msa1007230 MtrunA17_Chr4g0064231 43.243 74 42 0 124 197 145 218 4.01e-12 62.8
Msa1007230 MtrunA17_Chr2g0329371 47.541 61 32 0 136 196 143 203 1.00e-11 61.6
Msa1007230 MtrunA17_Chr7g0245371 44.928 69 38 0 127 195 102 170 1.02e-11 60.8
Msa1007230 MtrunA17_Chr4g0033601 43.939 66 37 0 131 196 77 142 1.31e-11 60.1
Msa1007230 MtrunA17_Chr8g0392951 44.118 68 36 1 130 197 94 159 1.49e-11 60.5
Msa1007230 MtrunA17_Chr7g0256171 45.902 61 33 0 136 196 82 142 9.64e-11 57.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1007230 AT5G39670.1 43.602 211 101 6 1 200 1 204 3.71e-46 151
Msa1007230 AT3G29000.1 45.109 184 88 4 24 197 11 191 2.97e-40 136
Msa1007230 AT3G47480.1 54.545 66 29 1 131 196 115 179 4.00e-16 73.2
Msa1007230 AT4G20780.1 54.930 71 29 1 125 195 116 183 1.86e-15 71.6
Msa1007230 AT5G44460.1 31.098 164 94 3 39 197 27 176 1.30e-14 69.3
Msa1007230 AT2G43290.1 40.449 89 52 1 108 196 120 207 5.91e-14 68.2
Msa1007230 AT1G05990.1 46.154 65 35 0 133 197 80 144 1.56e-12 62.8
Msa1007230 AT4G03290.1 45.312 64 35 0 133 196 82 145 4.46e-12 62.0
Msa1007230 AT1G21550.1 37.838 111 62 2 85 191 41 148 4.59e-12 62.0
Msa1007230 AT3G07490.1 45.902 61 33 0 136 196 82 142 7.96e-12 61.2
Msa1007230 AT3G59440.1 25.758 198 106 6 25 197 7 188 1.48e-11 61.2

Find 23 sgRNAs with CRISPR-Local

Find 33 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCGAAGAGTTATGTGTATTT+TGG 0.154565 7_1:+6662203 Msa1007230:CDS
TGAAGCGGTGATGGCGAAAA+TGG 0.258329 7_1:+6662020 Msa1007230:CDS
GAGAAAGTGGAGAGATTAAG+AGG 0.304916 7_1:+6661989 Msa1007230:CDS
GAAAGCAACTCAACTTTGTT+TGG 0.330887 7_1:+6661802 Msa1007230:CDS
CTTCTCTGCCAACTTCGTTC+CGG 0.355688 7_1:+6661898 Msa1007230:CDS
TGATGAGAATGAGCCAAGTT+TGG 0.401446 7_1:+6662113 Msa1007230:CDS
ATCTTCACTTCCTCCAAACT+TGG 0.413419 7_1:-6662126 None:intergenic
TTCCGGTGACTTAGAAGTAA+AGG 0.466313 7_1:+6661915 Msa1007230:CDS
CGGAACGAAGTTGGCAGAGA+AGG 0.482954 7_1:-6661897 None:intergenic
TCCGGTGACTTAGAAGTAAA+GGG 0.483748 7_1:+6661916 Msa1007230:CDS
GAAGCGGTGATGGCGAAAAT+GGG 0.492089 7_1:+6662021 Msa1007230:CDS
AAGTTTGGAGGAAGTGAAGA+TGG 0.496634 7_1:+6662128 Msa1007230:CDS
TCCCTTTACTTCTAAGTCAC+CGG 0.500276 7_1:-6661917 None:intergenic
TGAGAATGAGCCAAGTTTGG+AGG 0.510803 7_1:+6662116 Msa1007230:CDS
GGATGAAGTTGAAGCGGTGA+TGG 0.511527 7_1:+6662011 Msa1007230:CDS
CTAAGTCACCGGAACGAAGT+TGG 0.521372 7_1:-6661906 None:intergenic
TCAAATGTAAGCGGAGAAAG+TGG 0.530749 7_1:+6661976 Msa1007230:CDS
AGATGGATTTATTGATGCAA+TGG 0.533184 7_1:+6662176 Msa1007230:CDS
CTGCAATAAGATAAACAAGT+TGG 0.540643 7_1:-6661756 None:intergenic
AGAAAGTGGAGAGATTAAGA+GGG 0.546580 7_1:+6661990 Msa1007230:CDS
TAAGAGGGATGAAGTTGAAG+CGG 0.562793 7_1:+6662005 Msa1007230:CDS
ACTTGTTTATCTTATTGCAG+AGG 0.590641 7_1:+6661759 Msa1007230:CDS
AGCAGAGACTCAAATGTAAG+CGG 0.613770 7_1:+6661967 Msa1007230:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CATTGAATTTGTAAAAATTA+TGG + chr7_1:6662308-6662327 Msa1007230:CDS 15.0%
! GATGATCTTGAAGAAAAATA+TGG + chr7_1:6662066-6662085 Msa1007230:CDS 25.0%
! TGAAGAAAAATATGGTTCAA+AGG + chr7_1:6662074-6662093 Msa1007230:CDS 25.0%
!!! TATGTGTATTTTGGGATTTA+AGG + chr7_1:6662212-6662231 Msa1007230:CDS 25.0%
!! AAGATGATCAAGAATTTTGA+TGG + chr7_1:6662261-6662280 Msa1007230:CDS 25.0%
!!! AATTTTGATGGAAATCAAGA+TGG + chr7_1:6662273-6662292 Msa1007230:CDS 25.0%
CTGCAATAAGATAAACAAGT+TGG - chr7_1:6661759-6661778 None:intergenic 30.0%
ACTTGTTTATCTTATTGCAG+AGG + chr7_1:6661759-6661778 Msa1007230:CDS 30.0%
! ATAAGCTTTTCTCGAGTTTT+TGG + chr7_1:6661869-6661888 Msa1007230:CDS 30.0%
! GTTTTTGATGAGAACAAAGA+TGG + chr7_1:6662159-6662178 Msa1007230:CDS 30.0%
! AGATGGATTTATTGATGCAA+TGG + chr7_1:6662176-6662195 Msa1007230:CDS 30.0%
! CGAAGAGTTATGTGTATTTT+GGG + chr7_1:6662204-6662223 Msa1007230:CDS 30.0%
!!! TGTATTTTGGGATTTAAGGA+AGG + chr7_1:6662216-6662235 Msa1007230:CDS 30.0%
GAAAGCAACTCAACTTTGTT+TGG + chr7_1:6661802-6661821 Msa1007230:CDS 35.0%
AGAAAGTGGAGAGATTAAGA+GGG + chr7_1:6661990-6662009 Msa1007230:CDS 35.0%
! GCGAAGAGTTATGTGTATTT+TGG + chr7_1:6662203-6662222 Msa1007230:CDS 35.0%
TTCCGGTGACTTAGAAGTAA+AGG + chr7_1:6661915-6661934 Msa1007230:CDS 40.0%
TCCGGTGACTTAGAAGTAAA+GGG + chr7_1:6661916-6661935 Msa1007230:CDS 40.0%
TCCCTTTACTTCTAAGTCAC+CGG - chr7_1:6661920-6661939 None:intergenic 40.0%
AGCAGAGACTCAAATGTAAG+CGG + chr7_1:6661967-6661986 Msa1007230:CDS 40.0%
TCAAATGTAAGCGGAGAAAG+TGG + chr7_1:6661976-6661995 Msa1007230:CDS 40.0%
GAGAAAGTGGAGAGATTAAG+AGG + chr7_1:6661989-6662008 Msa1007230:CDS 40.0%
TAAGAGGGATGAAGTTGAAG+CGG + chr7_1:6662005-6662024 Msa1007230:CDS 40.0%
TGATGAGAATGAGCCAAGTT+TGG + chr7_1:6662113-6662132 Msa1007230:CDS 40.0%
ATCTTCACTTCCTCCAAACT+TGG - chr7_1:6662129-6662148 None:intergenic 40.0%
AAGTTTGGAGGAAGTGAAGA+TGG + chr7_1:6662128-6662147 Msa1007230:CDS 40.0%
TGAGAATGAGCCAAGTTTGG+AGG + chr7_1:6662116-6662135 Msa1007230:CDS 45.0%
CTTCTCTGCCAACTTCGTTC+CGG + chr7_1:6661898-6661917 Msa1007230:CDS 50.0%
CTAAGTCACCGGAACGAAGT+TGG - chr7_1:6661909-6661928 None:intergenic 50.0%
GGATGAAGTTGAAGCGGTGA+TGG + chr7_1:6662011-6662030 Msa1007230:CDS 50.0%
! TGAAGCGGTGATGGCGAAAA+TGG + chr7_1:6662020-6662039 Msa1007230:CDS 50.0%
! GAAGCGGTGATGGCGAAAAT+GGG + chr7_1:6662021-6662040 Msa1007230:CDS 50.0%
CGGAACGAAGTTGGCAGAGA+AGG - chr7_1:6661900-6661919 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr7_1 gene 6661745 6662347 6661745 ID=Msa1007230;Name=Msa1007230
chr7_1 mRNA 6661745 6662347 6661745 ID=Msa1007230-mRNA-1;Parent=Msa1007230;Name=Msa1007230-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|200
chr7_1 exon 6661745 6662347 6661745 ID=Msa1007230-mRNA-1:exon:1958;Parent=Msa1007230-mRNA-1
chr7_1 CDS 6661745 6662347 6661745 ID=Msa1007230-mRNA-1:cds;Parent=Msa1007230-mRNA-1
Gene Sequence

>Msa1007230

ATGGTTTTTCGCCAACTTGTTTATCTTATTGCAGAGGAGAACACATTACAGAATGATGAAAGCAACTCAACTTTGTTTGGTTTGATAGATTTTTTCATGTGTTCAGCATTATTCATTAGAATACATAAGCTTTTCTCGAGTTTTTGGTTTTTCCTTCTCTGCCAACTTCGTTCCGGTGACTTAGAAGTAAAGGGAGAGAAGCAGTTATCAGAAACTGAATCTAGCAGAGACTCAAATGTAAGCGGAGAAAGTGGAGAGATTAAGAGGGATGAAGTTGAAGCGGTGATGGCGAAAATGGGACTTTTTTGCAGCTCAGAAAGTGATGATCTTGAAGAAAAATATGGTTCAAAGGAGCTTTCTGAATTGTTTGATGAGAATGAGCCAAGTTTGGAGGAAGTGAAGATGGCTTTCGATGTTTTTGATGAGAACAAAGATGGATTTATTGATGCAATGGAGTTGCGAAGAGTTATGTGTATTTTGGGATTTAAGGAAGGTTCAGAAGTTAAGAGTTGTCACAAGATGATCAAGAATTTTGATGGAAATCAAGATGGAAGAATTGATTTCATTGAATTTGTAAAAATTATGGAAAACAGATTGTGTTGA

Protein sequence

>Msa1007230

MVFRQLVYLIAEENTLQNDESNSTLFGLIDFFMCSALFIRIHKLFSSFWFFLLCQLRSGDLEVKGEKQLSETESSRDSNVSGESGEIKRDEVEAVMAKMGLFCSSESDDLEEKYGSKELSELFDENEPSLEEVKMAFDVFDENKDGFIDAMELRRVMCILGFKEGSEVKSCHKMIKNFDGNQDGRIDFIEFVKIMENRLC*