AlfalfaGEDB | Msa0524320

M. sativa cultivar zhongmu-4 / Msa0524320

KEGG: K09872
GO: GO:0001101 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005215 GO:0005372 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005773 GO:0005886 GO:0005887 GO:0005911 GO:0006810 GO:0006811 GO:0006833 GO:0008150 GO:0009506 GO:0009719 GO:0009725 GO:0009737 GO:0010033 GO:0015250 GO:0015267 GO:0015318 GO:0016020 GO:0016021 GO:0022803 GO:0022838 GO:0022857 GO:0030054 GO:0031224 GO:0031225 GO:0031226 GO:0033993 GO:0034220 GO:0042044 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044425 GO:0044444 GO:0044459 GO:0044464 GO:0046658 GO:0050896 GO:0051179 GO:0051234 GO:0055044 GO:0055085 GO:0071944 GO:0097159 GO:0097305 GO:1901363 GO:1901700
PFAM: PF00230.23

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0524320	GAU33646.1	97.527	283	6	1	1	282	1	283	0.0	553

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0524320	sp\|P93004\|PIP27_ARATH	89.716	282	27	1	1	282	1	280	0.0	514

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0524320	A0A2Z6MMA4	97.527	283	6	1	1	282	1	283	0.0	553

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0006840	Msa0524320	0.806303	8.783413e-50	-8.615850e-47
Msa0015570	Msa0524320	0.805406	1.355554e-49	-8.615850e-47
Msa0016530	Msa0524320	0.803619	3.197352e-49	-8.615850e-47
Msa0018920	Msa0524320	0.818063	2.382808e-52	-8.615850e-47
Msa0027800	Msa0524320	0.807263	5.505864e-50	-8.615850e-47
Msa0030070	Msa0524320	0.840730	7.290114e-58	-8.615850e-47
Msa0030690	Msa0524320	0.812683	3.747847e-51	-8.615850e-47
Msa0032980	Msa0524320	0.882053	1.438203e-70	-8.615850e-47
Msa0033780	Msa0524320	0.831966	1.236797e-55	-8.615850e-47
Msa0040640	Msa0524320	0.824322	8.593273e-54	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0524320	MtrunA17_Chr3g0145671	97.163	282	5	1	1	282	1	279	0.0	548
Msa0524320	MtrunA17_Chr7g0264111	79.443	287	48	3	1	279	1	284	2.63e-162	452
Msa0524320	MtrunA17_Chr2g0326621	74.126	286	67	1	1	279	1	286	2.21e-156	437
Msa0524320	MtrunA17_Chr4g0029071	75.532	282	60	2	4	279	6	284	7.40e-156	436
Msa0524320	MtrunA17_Chr1g0199731	77.113	284	56	2	4	281	6	286	4.25e-155	434
Msa0524320	MtrunA17_Chr8g0386961	69.145	269	82	1	4	271	16	284	8.18e-134	380
Msa0524320	MtrunA17_Chr3g0113211	71.648	261	73	1	13	272	28	288	1.91e-133	379
Msa0524320	MtrunA17_Chr4g0006001	69.922	256	77	0	17	272	33	288	2.45e-133	379
Msa0524320	MtrunA17_Chr5g0436701	71.595	257	72	1	17	272	32	288	2.99e-131	374
Msa0524320	MtrunA17_Chr5g0397391	67.778	270	86	1	4	272	16	285	6.52e-130	370

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0524320	AT4G35100.1	89.716	282	27	1	1	282	1	280	0.0	514
Msa0524320	AT4G35100.2	89.716	282	27	1	1	282	1	280	0.0	514
Msa0524320	AT2G16850.1	90.780	282	22	1	1	282	1	278	0.0	513
Msa0524320	AT5G60660.1	75.000	284	64	2	1	278	1	283	2.96e-153	429
Msa0524320	AT3G54820.1	74.825	286	64	2	1	280	1	284	3.29e-152	427
Msa0524320	AT3G53420.1	77.985	268	53	1	17	278	16	283	6.57e-152	426
Msa0524320	AT3G53420.2	77.985	268	53	1	17	278	16	283	6.57e-152	426
Msa0524320	AT2G37170.2	73.611	288	66	3	1	278	37	324	1.04e-151	427
Msa0524320	AT2G37170.1	74.564	287	58	4	1	278	1	281	8.96e-151	423
Msa0524320	AT2G39010.1	74.126	286	64	3	1	279	1	283	1.22e-150	423

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Find 107 sgRNAs with CRISPR-Local

Find 236 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGGCCGAATGTGACAGC+AGG	0.520051	3_4:-77807196	None:intergenic
AAACAAACTGGTCCATGTGA+CGG	0.634416	3_4:+77807082	Msa0524320:CDS
AAACACCCTTACAACCACCT+CGG	0.690282	3_4:+77807316	Msa0524320:CDS
AAAGGCCGAATGTGACAGCA+GGG	0.654056	3_4:-77807195	None:intergenic
AAGTCAAGGAGGGGTGCTGG+TGG	0.564356	3_4:-77806956	None:intergenic
AATCTCAGCGAAGTCAAGGA+GGG	0.616287	3_4:-77806966	None:intergenic
AATTAACTAATTTAGTTGGT+GGG	0.430160	3_4:-77807827	None:intergenic
AATTAATTAACTAATTTAGT+TGG	0.361626	3_4:-77807831	None:intergenic
ACACTTTACGAGCCAAGAAA+AGG	0.506621	3_4:-77807213	None:intergenic
ACAGCAAAACCAATGGGCAA+AGG	0.573216	3_4:-77807487	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATTGGTCACAAAAAACAAAC+TGG	+	chr3_4:77807070-77807089	Msa0524320:CDS	30.0%
CATAAAAACAGCAAAACCAA+TGG	-	chr3_4:77807497-77807516	None:intergenic	30.0%
TATACTATATATGCATGCAG+TGG	+	chr3_4:77807699-77807718	Msa0524320:intron	30.0%
ATTGGTCACAAAAAACAAAC+TGG	+	chr3_4:77807070-77807089	Msa0524320:CDS	30.0%
CATAAAAACAGCAAAACCAA+TGG	-	chr3_4:77807497-77807516	None:intergenic	30.0%
TATACTATATATGCATGCAG+TGG	+	chr3_4:77807699-77807718	Msa0524320:intron	30.0%
TCTAAAGAAGTGAGTGAAGA+AGG	+	chr3_4:77806893-77806912	Msa0524320:CDS	35.0%
CTAAAGAAGTGAGTGAAGAA+GGG	+	chr3_4:77806894-77806913	Msa0524320:CDS	35.0%
TCTTCAACAATGCCAAAGTT+TGG	+	chr3_4:77807587-77807606	Msa0524320:CDS	35.0%
CTTCAACAATGCCAAAGTTT+GGG	+	chr3_4:77807588-77807607	Msa0524320:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3_4	gene	77806849	77808076	77806849	ID=Msa0524320;Name=Msa0524320
chr3_4	mRNA	77806849	77808076	77806849	ID=Msa0524320-mRNA-1;Parent=Msa0524320;Name=Msa0524320-mRNA-1;_AED=0.18;_eAED=0.18;_QI=41\|1\|1\|1\|1\|1\|2\|238\|282
chr3_4	exon	77806849	77807618	77806849	ID=Msa0524320-mRNA-1:exon:22856;Parent=Msa0524320-mRNA-1
chr3_4	exon	77807719	77808076	77807719	ID=Msa0524320-mRNA-1:exon:22857;Parent=Msa0524320-mRNA-1
chr3_4	five_prime_UTR	77806849	77806889	77806849	ID=Msa0524320-mRNA-1:five_prime_utr;Parent=Msa0524320-mRNA-1
chr3_4	CDS	77806890	77807618	77806890	ID=Msa0524320-mRNA-1:cds;Parent=Msa0524320-mRNA-1
chr3_4	CDS	77807719	77807838	77807719	ID=Msa0524320-mRNA-1:cds;Parent=Msa0524320-mRNA-1
chr3_4	three_prime_UTR	77807839	77808076	77807839	ID=Msa0524320-mRNA-1:three_prime_utr;Parent=Msa0524320-mRNA-1

Gene Sequence

>Msa0524320

ATGTCTAAAGAAGTGAGTGAAGAAGGGCACCTACCACATCCTCACGGTAAGGACTACGTAGACCCACCACCAGCACCCCTCCTTGACTTCGCTGAGATTAAGCTCTGGTCTTTCTACAGAGCTCTCATCGCTGAGTTCATAGCCACCCTCCTCTTTCTCTACGTCACTGTCGCCACCGTCATTGGTCACAAAAAACAAACTGGTCCATGTGACGGTGTTGGCCTTCTAGGCATAGCATGGTCCTTTGGTGGCATGATCTTTGTCCTTGTCTACTGCACCGCTGGTATCTCCGGTGGCCACATTAACCCTGCTGTCACATTCGGCCTTTTCTTGGCTCGTAAAGTGTCTCTCATAAGAGCGGTGTTATACATGGTAGCACAGTGTTTAGGTGCTATCTGTGGCGTTGGGTTGGTGAAGGCTTTCATGAAACACCCTTACAACCACCTCGGCGGCGGTGCTAACTCTGTAGCTTCCGGTTACAGCAAAGGCTCTGCCCTCGGTGCTGAAATTATCGGAACTTTTGTGTTGGTTTACACTGTTTTCTCTGCTACTGACCCTAAGAGAAGCGCACGGGACTCACATGTCCCTGTTTTGGCTCCTTTGCCCATTGGTTTTGCTGTTTTTATGGTTCACCTTGCAACTATACCAATCACCGGTACCGGAATCAACCCTGCTAGGAGCTTCGGTGCTGCTGTCATCTTCAACAATGCCAAAGTTTGGGATGACCATTGGATCTTCTGGGTTGGACCGTTGGTGGGAGCGTTGGCAGCGGCTGCATACCATCAATACATTCTTAGAGCGGCGGCTATCAAGGCGTTGGGCTCCTTTAGAAGCAACCCCACCAACTAA

Protein sequence

>Msa0524320

MSKEVSEEGHLPHPHGKDYVDPPPAPLLDFAEIKLWSFYRALIAEFIATLLFLYVTVATVIGHKKQTGPCDGVGLLGIAWSFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVSLIRAVLYMVAQCLGAICGVGLVKAFMKHPYNHLGGGANSVASGYSKGSALGAEIIGTFVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIFNNAKVWDDHWIFWVGPLVGALAAAAYHQYILRAAAIKALGSFRSNPTN*