AlfalfaGEDB | Msa1424590

M. sativa cultivar zhongmu-4 / Msa1424590

KEGG: K09872
GO: GO:0001101 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005215 GO:0005372 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005773 GO:0005886 GO:0005887 GO:0005911 GO:0006810 GO:0006811 GO:0006833 GO:0008150 GO:0009506 GO:0009719 GO:0009725 GO:0009737 GO:0010033 GO:0015250 GO:0015267 GO:0015318 GO:0016020 GO:0016021 GO:0022803 GO:0022838 GO:0022857 GO:0030054 GO:0031224 GO:0031225 GO:0031226 GO:0033993 GO:0034220 GO:0042044 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044425 GO:0044444 GO:0044459 GO:0044464 GO:0046658 GO:0050896 GO:0051179 GO:0051234 GO:0055044 GO:0055085 GO:0071944 GO:0097159 GO:0097305 GO:1901363 GO:1901700
PFAM: PF00230.23

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1424590	sp\|P93004\|PIP27_ARATH	89.716	282	27	1	1	282	1	280	0.0	514

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1424590	A0A2Z6MMA4	97.527	283	6	1	1	282	1	283	0.0	553

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0006840	Msa1424590	0.816948	4.250230e-52	-8.615850e-47
Msa0018920	Msa1424590	0.813205	2.880097e-51	-8.615850e-47
Msa0027800	Msa1424590	0.821830	3.277634e-53	-8.615850e-47
Msa0030070	Msa1424590	0.844921	5.604200e-59	-8.615850e-47
Msa0030690	Msa1424590	0.815158	1.066800e-51	-8.615850e-47
Msa0032970	Msa1424590	0.800281	1.551586e-48	-8.615850e-47
Msa0032980	Msa1424590	0.895942	5.927762e-76	-8.615850e-47
Msa0033780	Msa1424590	0.828031	1.126693e-54	-8.615850e-47
Msa0040640	Msa1424590	0.832310	1.016656e-55	-8.615850e-47
Msa0049450	Msa1424590	0.815675	8.186301e-52	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1424590	MtrunA17_Chr3g0145671	97.163	282	5	1	1	282	1	279	0.0	548
Msa1424590	MtrunA17_Chr7g0264111	79.443	287	48	3	1	279	1	284	2.63e-162	452
Msa1424590	MtrunA17_Chr2g0326621	74.126	286	67	1	1	279	1	286	2.21e-156	437
Msa1424590	MtrunA17_Chr4g0029071	75.532	282	60	2	4	279	6	284	7.40e-156	436
Msa1424590	MtrunA17_Chr1g0199731	77.113	284	56	2	4	281	6	286	4.25e-155	434
Msa1424590	MtrunA17_Chr8g0386961	69.145	269	82	1	4	271	16	284	8.18e-134	380
Msa1424590	MtrunA17_Chr3g0113211	71.648	261	73	1	13	272	28	288	1.91e-133	379
Msa1424590	MtrunA17_Chr4g0006001	69.922	256	77	0	17	272	33	288	2.45e-133	379
Msa1424590	MtrunA17_Chr5g0436701	71.595	257	72	1	17	272	32	288	2.99e-131	374
Msa1424590	MtrunA17_Chr5g0397391	67.778	270	86	1	4	272	16	285	6.52e-130	370

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1424590	AT4G35100.1	89.716	282	27	1	1	282	1	280	0.0	514
Msa1424590	AT4G35100.2	89.716	282	27	1	1	282	1	280	0.0	514
Msa1424590	AT2G16850.1	90.780	282	22	1	1	282	1	278	0.0	513
Msa1424590	AT5G60660.1	75.000	284	64	2	1	278	1	283	2.96e-153	429
Msa1424590	AT3G54820.1	74.825	286	64	2	1	280	1	284	3.29e-152	427
Msa1424590	AT3G53420.1	77.985	268	53	1	17	278	16	283	6.57e-152	426
Msa1424590	AT3G53420.2	77.985	268	53	1	17	278	16	283	6.57e-152	426
Msa1424590	AT2G37170.2	73.611	288	66	3	1	278	37	324	1.04e-151	427
Msa1424590	AT2G37170.1	74.564	287	58	4	1	278	1	281	8.96e-151	423
Msa1424590	AT2G39010.1	74.126	286	64	3	1	279	1	283	1.22e-150	423

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Find 107 sgRNAs with CRISPR-Local

Find 236 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGGCCGAATGTGACAGC+AGG	0.520051	tig0025770:+43198	None:intergenic
AAACAAACTGGTCCATGTGA+CGG	0.634416	tig0025770:-43312	Msa1424590:CDS
AAACACCCTTACAACCACCT+CGG	0.690282	tig0025770:-43078	Msa1424590:CDS
AAAGGCCGAATGTGACAGCA+GGG	0.654056	tig0025770:+43199	None:intergenic
AAGTCAAGGAGGGGTGCTGG+TGG	0.564356	tig0025770:+43438	None:intergenic
AATCTCAGCGAAGTCAAGGA+GGG	0.616287	tig0025770:+43428	None:intergenic
AATTAATTAATTAATTTAGT+TGG	0.338910	tig0025770:+42563	None:intergenic
AATTAATTAATTTAGTTGGT+GGG	0.430160	tig0025770:+42567	None:intergenic
ACACTTTACGAGCCAAGAAA+AGG	0.506621	tig0025770:+43181	None:intergenic
ACAGCAAAACCAATGGGCAA+AGG	0.573216	tig0025770:+42907	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATTGGTCACAAAAAACAAAC+TGG	-	tig0025770:42574-42593	Msa1424590:exon	30.0%
CATAAAAACAGCAAAACCAA+TGG	+	tig0025770:43001-43020	None:intergenic	30.0%
TATACTATATATGCATGCAG+TGG	-	tig0025770:43203-43222	Msa1424590:CDS	30.0%
ATTGGTCACAAAAAACAAAC+TGG	-	tig0025770:42574-42593	Msa1424590:exon	30.0%
CATAAAAACAGCAAAACCAA+TGG	+	tig0025770:43001-43020	None:intergenic	30.0%
TATACTATATATGCATGCAG+TGG	-	tig0025770:43203-43222	Msa1424590:CDS	30.0%
TCTAAAGAAGTGAGTGAAGA+AGG	-	tig0025770:42397-42416	Msa1424590:three_prime_UTR	35.0%
CTAAAGAAGTGAGTGAAGAA+GGG	-	tig0025770:42398-42417	Msa1424590:three_prime_UTR	35.0%
TCTTCAACAATGCCAAAGTT+TGG	-	tig0025770:43091-43110	Msa1424590:CDS	35.0%
CTTCAACAATGCCAAAGTTT+GGG	-	tig0025770:43092-43111	Msa1424590:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0025770	gene	42340	43580	42340	ID=Msa1424590;Name=Msa1424590
tig0025770	mRNA	42340	43580	42340	ID=Msa1424590-mRNA-1;Parent=Msa1424590;Name=Msa1424590-mRNA-1;_AED=0.02;_eAED=0.02;_QI=54\|1\|1\|1\|1\|1\|2\|238\|282
tig0025770	exon	42340	42697	42340	ID=Msa1424590-mRNA-1:exon:3847;Parent=Msa1424590-mRNA-1
tig0025770	exon	42798	43580	42798	ID=Msa1424590-mRNA-1:exon:3846;Parent=Msa1424590-mRNA-1
tig0025770	five_prime_UTR	43527	43580	43527	ID=Msa1424590-mRNA-1:five_prime_utr;Parent=Msa1424590-mRNA-1
tig0025770	CDS	42798	43526	42798	ID=Msa1424590-mRNA-1:cds;Parent=Msa1424590-mRNA-1
tig0025770	CDS	42578	42697	42578	ID=Msa1424590-mRNA-1:cds;Parent=Msa1424590-mRNA-1
tig0025770	three_prime_UTR	42340	42577	42340	ID=Msa1424590-mRNA-1:three_prime_utr;Parent=Msa1424590-mRNA-1

Gene Sequence

>Msa1424590

ATGTCTAAAGAAGTGAGTGAAGAAGGGCACCTACCACATCCTCACGGTAAGGACTACGTAGACCCACCACCAGCACCCCTCCTTGACTTCGCTGAGATTAAGCTCTGGTCTTTCTACAGAGCTCTCATCGCTGAGTTCATAGCCACCCTCCTCTTTCTCTACGTCACTGTCGCCACCGTCATTGGTCACAAAAAACAAACTGGTCCATGTGACGGTGTTGGCCTTCTAGGCATAGCATGGTCCTTTGGTGGCATGATCTTTGTCCTTGTCTACTGCACCGCTGGTATCTCCGGTGGCCACATTAACCCTGCTGTCACATTCGGCCTTTTCTTGGCTCGTAAAGTGTCTCTCATAAGAGCGGTGTTATACATGGTAGCACAGTGTTTAGGTGCTATCTGTGGCGTTGGGTTGGTGAAGGCTTTCATGAAACACCCTTACAACCACCTCGGCGGCGGTGCTAACTCTGTAGCTTCCGGTTACAGCAAAGGCTCTGCCCTCGGTGCTGAAATTATCGGAACTTTTGTGTTGGTTTACACTGTTTTCTCTGCTACTGACCCTAAGAGAAGCGCACGGGACTCACATGTCCCTGTTTTGGCTCCTTTGCCCATTGGTTTTGCTGTTTTTATGGTTCACCTTGCAACTATACCAATCACCGGTACCGGAATCAACCCTGCTAGGAGCTTCGGTGCTGCTGTCATCTTCAACAATGCCAAAGTTTGGGATGACCATTGGATCTTCTGGGTTGGACCGTTGGTGGGAGCGTTGGCAGCGGCTGCATACCATCAATACATTCTTAGAGCGGCGGCTATCAAGGCGTTGGGCTCCTTTAGAAGCAACCCCACCAACTAA

Protein sequence

>Msa1424590

MSKEVSEEGHLPHPHGKDYVDPPPAPLLDFAEIKLWSFYRALIAEFIATLLFLYVTVATVIGHKKQTGPCDGVGLLGIAWSFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVSLIRAVLYMVAQCLGAICGVGLVKAFMKHPYNHLGGGANSVASGYSKGSALGAEIIGTFVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIFNNAKVWDDHWIFWVGPLVGALAAAAYHQYILRAAAIKALGSFRSNPTN*