AlfalfaGEDB | Msa0400650

M. sativa cultivar zhongmu-4 / Msa0400650

KEGG: K22390
GO: GO:0003674 GO:0003824 GO:0003993 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0042578 GO:0044237
PFAM: PF16656.8

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0400650	XP_003600057.1	96.648	358	12	0	22	379	45	402	0.0	732

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0400650	sp\|Q9LJU7\|PPA18_ARATH	76.819	371	80	1	22	386	49	419	0.0	617

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0400650	G7IXK7	96.648	358	12	0	22	379	45	402	0.0	732

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0019900	Msa0400650	0.815854	7.467360e-52	-8.615850e-47
Msa0025160	Msa0400650	0.841094	5.853066e-58	-8.615850e-47
Msa0072520	Msa0400650	0.822844	1.905355e-53	-8.615850e-47
Msa0161930	Msa0400650	0.802364	5.809355e-49	-8.615850e-47
Msa0226310	Msa0400650	0.801976	6.981827e-49	-8.615850e-47
Msa0284800	Msa0400650	0.831245	1.861767e-55	-8.615850e-47
Msa0304680	Msa0400650	0.811240	7.727404e-51	-8.615850e-47
Msa0330520	Msa0400650	0.818000	2.462833e-52	-8.615850e-47
Msa0334660	Msa0400650	0.821380	4.163485e-53	-8.615850e-47
Msa0400650	Msa0453040	0.866719	2.321938e-65	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0400650	MtrunA17_Chr3g0098721	96.648	358	12	0	22	379	45	402	0.0	732
Msa0400650	MtrunA17_Chr5g0443881	83.473	357	59	0	22	378	51	407	0.0	650
Msa0400650	MtrunA17_Chr1g0178831	65.363	358	123	1	22	378	48	405	0.0	509
Msa0400650	MtrunA17_Chr7g0266131	63.966	358	128	1	22	378	56	413	2.40e-179	506
Msa0400650	MtrunA17_Chr7g0266151	60.218	367	142	3	22	385	49	414	1.61e-161	460
Msa0400650	MtrunA17_Chr4g0064541	44.357	381	185	7	22	376	57	436	1.54e-98	301
Msa0400650	MtrunA17_Chr6g0453731	39.189	444	178	8	22	376	73	513	4.98e-95	295
Msa0400650	MtrunA17_Chr3g0121411	39.159	452	182	8	14	376	67	514	5.30e-94	291
Msa0400650	MtrunA17_Chr7g0236041	38.876	445	179	8	22	376	72	513	2.71e-93	290
Msa0400650	MtrunA17_Chr4g0019091	42.932	382	190	9	22	376	57	437	1.24e-92	285

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0400650	AT3G20500.1	76.819	371	80	1	22	386	49	419	0.0	617
Msa0400650	AT3G52820.1	60.938	384	143	2	8	384	35	418	3.40e-178	503
Msa0400650	AT3G52810.1	60.218	367	145	1	22	387	53	419	1.22e-165	471
Msa0400650	AT3G52780.1	62.431	362	129	5	22	377	46	406	2.45e-164	467
Msa0400650	AT3G52780.2	61.644	292	105	5	22	307	46	336	1.08e-127	372
Msa0400650	AT5G34850.1	43.307	381	189	7	22	376	56	435	8.69e-97	297
Msa0400650	AT3G07130.1	41.320	409	167	5	40	376	97	504	5.04e-94	291
Msa0400650	AT1G56360.1	41.538	390	195	10	22	379	52	440	8.73e-91	281
Msa0400650	AT4G36350.1	41.538	390	194	11	22	379	53	440	5.51e-90	279
Msa0400650	AT3G07130.2	38.235	442	167	6	40	376	97	537	3.88e-87	275

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Find 126 sgRNAs with CRISPR-Local

Find 500 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATGCCATTTGAGGAAAG+TGG	0.586799	3_2:+25130756	Msa0400650:CDS
AAACTAATTGGGAATTGAGC+TGG	0.540026	3_2:-25130477	None:intergenic
AAACTATCGTGGAGAGCTTA+TGG	0.300724	3_2:-25130080	None:intergenic
AACACAGCTCATCAAGGTGA+AGG	0.620113	3_2:+25132599	Msa0400650:CDS
AACATCAATGGACTAGACTA+CGG	0.574203	3_2:-25129598	None:intergenic
AAGATACACCATACGGTAAT+AGG	0.307628	3_2:+25130381	Msa0400650:CDS
AAGTATGACTCGGTAGCTGA+AGG	0.586262	3_2:+25130318	Msa0400650:CDS
AAGTATTATAGACTTGTGTC+TGG	0.530246	3_2:+25129675	Msa0400650:five_prime_UTR
AATAGTAACACAGCTCATCA+AGG	0.589055	3_2:+25132593	Msa0400650:CDS
AATAGTTCTGATTTATTGTG+AGG	0.507350	3_2:+25129367	Msa0400650:five_prime_UTR

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATTGCACTAAATGATCTATG+AGG	-	chr3_2:25129258-25129277	None:intergenic	30.0%
AGATCAGACGACTTATATTT+AGG	+	chr3_2:25129476-25129495	Msa0400650:five_prime_UTR	30.0%
CTTATGAAGTTATGAATGTG+TGG	+	chr3_2:25129567-25129586	Msa0400650:five_prime_UTR	30.0%
AAGTATTATAGACTTGTGTC+TGG	+	chr3_2:25129675-25129694	Msa0400650:five_prime_UTR	30.0%
ATGAGAATCGGAAAAATTAG+AGG	-	chr3_2:25129721-25129740	None:intergenic	30.0%
TCATCCCTAAATATGAGAAT+CGG	-	chr3_2:25129733-25129752	None:intergenic	30.0%
TTATATGAAAAGTGGAATCC+AGG	+	chr3_2:25130197-25130216	Msa0400650:CDS	30.0%
GTAGAAAATGTAACTGTAAG+AGG	-	chr3_2:25130352-25130371	None:intergenic	30.0%
CGGTAAAAGTAAACAGAATT+CGG	-	chr3_2:25130417-25130436	None:intergenic	30.0%
AGAGCATATACCTTTGTTAA+AGG	+	chr3_2:25130704-25130723	Msa0400650:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3_2	gene	25129239	25133351	25129239	ID=Msa0400650;Name=Msa0400650
chr3_2	mRNA	25129239	25133351	25129239	ID=Msa0400650-mRNA-1;Parent=Msa0400650;Name=Msa0400650-mRNA-1;_AED=0.10;_eAED=0.10;_QI=914\|0.75\|0.6\|0.8\|1\|1\|5\|0\|387
chr3_2	exon	25129239	25130887	25129239	ID=Msa0400650-mRNA-1:exon:5234;Parent=Msa0400650-mRNA-1
chr3_2	exon	25132524	25132712	25132524	ID=Msa0400650-mRNA-1:exon:5235;Parent=Msa0400650-mRNA-1
chr3_2	exon	25132802	25132890	25132802	ID=Msa0400650-mRNA-1:exon:5236;Parent=Msa0400650-mRNA-1
chr3_2	exon	25133029	25133152	25133029	ID=Msa0400650-mRNA-1:exon:5237;Parent=Msa0400650-mRNA-1
chr3_2	exon	25133325	25133351	25133325	ID=Msa0400650-mRNA-1:exon:5238;Parent=Msa0400650-mRNA-1
chr3_2	five_prime_UTR	25129239	25130152	25129239	ID=Msa0400650-mRNA-1:five_prime_utr;Parent=Msa0400650-mRNA-1
chr3_2	CDS	25130153	25130887	25130153	ID=Msa0400650-mRNA-1:cds;Parent=Msa0400650-mRNA-1
chr3_2	CDS	25132524	25132712	25132524	ID=Msa0400650-mRNA-1:cds;Parent=Msa0400650-mRNA-1
chr3_2	CDS	25132802	25132890	25132802	ID=Msa0400650-mRNA-1:cds;Parent=Msa0400650-mRNA-1
chr3_2	CDS	25133029	25133152	25133029	ID=Msa0400650-mRNA-1:cds;Parent=Msa0400650-mRNA-1
chr3_2	CDS	25133325	25133351	25133325	ID=Msa0400650-mRNA-1:cds;Parent=Msa0400650-mRNA-1

Gene Sequence

>Msa0400650

ATGTTATTTTCTGACACTATAATTTCATATATTTATTCAGATTCTTATATGAAAAGTGGAATCCAGGTACACATTTCTTTGGCAGGAGACAAGCACGTGAGAATCACCTGGGTTACTGATGACAAATCTGCACCTTCAGTTGTAGAATATGGAACATTGCCAGGGAAGTATGACTCGGTAGCTGAAGGAGAGACAACCTCTTACAGTTACATTTTCTACAGCTCAGGAAAGATACACCATACGGTAATAGGTCCTTTAGAGCCGAATTCTGTTTACTTTTACCGATGCGGTGGACAAGGTCCTGAGCTCCAGCTCAAAACACCACCAGCTCAATTCCCAATTAGTTTTGCTGTGGTCGGAGATCTTGGCCAAACTGGTTGGACCAAATCAACATTAGATCACATCGACCAATGCAGATACGATGTTAACCTGATTCCTGGCGACCTTTCGTATGCCGACTATATACAGCATCGTTGGGACACGTTTGGTAGGCTTGTGCAGCCACTTGCTAGTTCTAGACCATGGATGGTAACACAAGGGAATCATGAAGTAGAGCATATACCTTTGTTAAAGGATGGATTTATATCCTATAATTCGAGGTGGAAAATGCCATTTGAGGAAAGTGGATCAAGCTCGAATCTCTATTATTCTTTTGAAGTTGCTGGTGCTCACATTATCATGCTTGGTTCATATGATGATTATGACGTGTACTCAGAACAATATAAATGGCTAAAGATGGATCTGTCAAAGGTGGACAGGAAAAGGACACCTTGGTTGCTTGTCATATTTCATGTGCCATGGTATAATAGTAACACAGCTCATCAAGGTGAAGGTGGTGATATGATGAAAACCATGGAGCCATTGCTTTATGCTGCTAGCGTCGATTTAGTTTTTGCAGGTCATGTCCACGCATATGAACGCTCGAAACGTGTATATAATGGAAAATTGGATCCTTGTGGTGCTGTTCATGTAACAATTGGTGATGGAGGGAACAAAGAAGGATTGGCTCATAAGTATATAGATCCACCGCCAAAGTGGTCAGAATTCCGTGAAGCAAGTTTTGGTCATGGTGAGCTAAAGATTGTAAACTCAACACATGCCTTCTGGAGTTGGCACCGGAATGACGATGATGAGCCGGGAATTGATAATCTTGTCAACCTCTAG

Protein sequence

>Msa0400650

MLFSDTIISYIYSDSYMKSGIQVHISLAGDKHVRITWVTDDKSAPSVVEYGTLPGKYDSVAEGETTSYSYIFYSSGKIHHTVIGPLEPNSVYFYRCGGQGPELQLKTPPAQFPISFAVVGDLGQTGWTKSTLDHIDQCRYDVNLIPGDLSYADYIQHRWDTFGRLVQPLASSRPWMVTQGNHEVEHIPLLKDGFISYNSRWKMPFEESGSSSNLYYSFEVAGAHIIMLGSYDDYDVYSEQYKWLKMDLSKVDRKRTPWLLVIFHVPWYNSNTAHQGEGGDMMKTMEPLLYAASVDLVFAGHVHAYERSKRVYNGKLDPCGAVHVTIGDGGNKEGLAHKYIDPPPKWSEFREASFGHGELKIVNSTHAFWSWHRNDDDEPGIDNLVNL*