Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0431360 | RHN70500.1 | 97.500 | 120 | 3 | 0 | 1 | 120 | 24 | 143 | 1.25e-75 | 230 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0431360 | A0A396IYB7 | 97.500 | 120 | 3 | 0 | 1 | 120 | 24 | 143 | 5.98e-76 | 230 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0175100 | Msa0431360 | 0.815461 | 9.135264e-52 | -8.615850e-47 |
| Msa0224980 | Msa0431360 | 0.810618 | 1.054010e-50 | -8.615850e-47 |
| Msa0225030 | Msa0431360 | 0.810359 | 1.198628e-50 | -8.615850e-47 |
| Msa0370180 | Msa0431360 | 0.806042 | 9.965247e-50 | -8.615850e-47 |
| Msa0379110 | Msa0431360 | 0.803099 | 4.096766e-49 | -8.615850e-47 |
| Msa0381970 | Msa0431360 | 0.968214 | 2.325993e-128 | -8.615850e-47 |
| Msa0431310 | Msa0431360 | 0.804855 | 1.768116e-49 | -8.615850e-47 |
| Msa0431360 | Msa0477780 | 0.807273 | 5.477884e-50 | -8.615850e-47 |
| Msa0431360 | Msa0565930 | 0.805584 | 1.244104e-49 | -8.615850e-47 |
| Msa0431360 | Msa0798500 | 0.817383 | 3.392101e-52 | -8.615850e-47 |
| Msa0431360 | Msa0851870 | 0.806485 | 8.040414e-50 | -8.615850e-47 |
| Msa0431360 | Msa1017590 | 0.815842 | 7.515062e-52 | -8.615850e-47 |
| Msa0431360 | Msa1116010 | 0.818115 | 2.320009e-52 | -8.615850e-47 |
| Msa0431360 | Msa1143910 | 0.819894 | 9.135898e-53 | -8.615850e-47 |
| Msa0431360 | Msa1359950 | 0.810859 | 9.347499e-51 | -8.615850e-47 |
| Msa0431360 | Msa1460650 | 0.800362 | 1.493162e-48 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0431360 | MtrunA17_Chr3g0136171 | 97.500 | 120 | 3 | 0 | 1 | 120 | 24 | 143 | 1.15e-79 | 230 |
| Msa0431360 | MtrunA17_Chr1g0150841 | 66.129 | 124 | 37 | 2 | 1 | 119 | 1 | 124 | 5.72e-48 | 149 |
| Msa0431360 | MtrunA17_Chr4g0070691 | 57.851 | 121 | 44 | 5 | 1 | 119 | 1 | 116 | 3.70e-35 | 117 |
| Msa0431360 | MtrunA17_Chr2g0300351 | 55.738 | 122 | 48 | 3 | 1 | 119 | 1 | 119 | 1.72e-24 | 90.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0431360 | AT2G06520.1 | 52.427 | 103 | 46 | 1 | 20 | 119 | 13 | 115 | 2.24e-24 | 90.1 |
Find 22 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTATACTTGGAGCTGTTAT+TGG | 0.192002 | 3_2:-83414731 | Msa0431360:CDS |
| TTTGACTGTGTGATCCTCTT+TGG | 0.259959 | 3_2:+83414929 | None:intergenic |
| CTATACTTGGAGCTGTTATT+GGG | 0.283981 | 3_2:-83414730 | Msa0431360:CDS |
| TTTGGTTGTGTGATGGGTAA+TGG | 0.333839 | 3_2:+83414947 | None:intergenic |
| CTTAATTAAGCTCTCTTAAC+CGG | 0.367220 | 3_2:+83414692 | None:intergenic |
| AGATGCATTAATTTCAACCT+TGG | 0.419109 | 3_2:+83414904 | None:intergenic |
| TAATGCATCTTTGAAAGAAA+AGG | 0.433518 | 3_2:-83414891 | Msa0431360:CDS |
| CCTGATGTGGCTCATGCAGC+TGG | 0.437171 | 3_2:-83414821 | Msa0431360:CDS |
| ATCCTCTTTGGTTGTGTGAT+GGG | 0.438365 | 3_2:+83414941 | None:intergenic |
| GATCCTCTTTGGTTGTGTGA+TGG | 0.472817 | 3_2:+83414940 | None:intergenic |
| TGGGGTTTCCAACTTTGATC+CGG | 0.483190 | 3_2:-83414711 | Msa0431360:CDS |
| CCAGCTGCATGAGCCACATC+AGG | 0.505992 | 3_2:+83414821 | None:intergenic |
| CTCCATGGTTATACCTGATG+TGG | 0.524546 | 3_2:-83414834 | Msa0431360:CDS |
| TCTCTTAACCGGATCAAAGT+TGG | 0.532912 | 3_2:+83414703 | None:intergenic |
| TGCATCCTTGACAGCCTCCA+TGG | 0.539158 | 3_2:-83414849 | Msa0431360:CDS |
| GCTGTGCTAACAGCTATACT+TGG | 0.540967 | 3_2:-83414743 | Msa0431360:CDS |
| TATACTTGGAGCTGTTATTG+GGG | 0.562747 | 3_2:-83414729 | Msa0431360:CDS |
| TATAACCATGGAGGCTGTCA+AGG | 0.564152 | 3_2:+83414844 | None:intergenic |
| TACCCATCACACAACCAAAG+AGG | 0.615865 | 3_2:-83414943 | Msa0431360:CDS |
| GATCACACAGTCAAAATCCA+AGG | 0.634917 | 3_2:-83414921 | Msa0431360:CDS |
| AGCCACATCAGGTATAACCA+TGG | 0.670542 | 3_2:+83414832 | None:intergenic |
| CACATCAGGTATAACCATGG+AGG | 0.712255 | 3_2:+83414835 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAATGCATCTTTGAAAGAAA+AGG | - | chr3_2:83414843-83414862 | Msa0431360:CDS | 25.0% |
| !! | TGGATTTTTCAGCTTGTTTT+GGG | + | chr3_2:83414746-83414765 | None:intergenic | 30.0% |
| !! | ATGGATTTTTCAGCTTGTTT+TGG | + | chr3_2:83414747-83414766 | None:intergenic | 30.0% |
| TAATGGCCTGAAAAATGATT+CGG | + | chr3_2:83414773-83414792 | None:intergenic | 30.0% | |
| AGATGCATTAATTTCAACCT+TGG | + | chr3_2:83414833-83414852 | None:intergenic | 30.0% | |
| AAATGCTTAGCAAAAAGTTC+TGG | + | chr3_2:83414960-83414979 | None:intergenic | 30.0% | |
| ! | TTTTTGCTAAGCATTTTTGC+TGG | - | chr3_2:83414964-83414983 | Msa0431360:CDS | 30.0% |
| CTATACTTGGAGCTGTTATT+GGG | - | chr3_2:83415004-83415023 | Msa0431360:CDS | 35.0% | |
| TATACTTGGAGCTGTTATTG+GGG | - | chr3_2:83415005-83415024 | Msa0431360:CDS | 35.0% | |
| ! | CTGAAAAATGATTCGGAGGA+TGG | + | chr3_2:83414766-83414785 | None:intergenic | 40.0% |
| !! | CATCCTCCGAATCATTTTTC+AGG | - | chr3_2:83414764-83414783 | Msa0431360:CDS | 40.0% |
| !! | TTTGGTTGTGTGATGGGTAA+TGG | + | chr3_2:83414790-83414809 | None:intergenic | 40.0% |
| ATCCTCTTTGGTTGTGTGAT+GGG | + | chr3_2:83414796-83414815 | None:intergenic | 40.0% | |
| TTTGACTGTGTGATCCTCTT+TGG | + | chr3_2:83414808-83414827 | None:intergenic | 40.0% | |
| GATCACACAGTCAAAATCCA+AGG | - | chr3_2:83414813-83414832 | Msa0431360:CDS | 40.0% | |
| !! | AGCAAAAAGTTCTGGAGAGA+TGG | + | chr3_2:83414952-83414971 | None:intergenic | 40.0% |
| ! | TTGCTAAGCATTTTTGCTGG+TGG | - | chr3_2:83414967-83414986 | Msa0431360:CDS | 40.0% |
| GCTATACTTGGAGCTGTTAT+TGG | - | chr3_2:83415003-83415022 | Msa0431360:CDS | 40.0% | |
| TCTCTTAACCGGATCAAAGT+TGG | + | chr3_2:83415034-83415053 | None:intergenic | 40.0% | |
| !! | TTTTGGGTAGCAGAACTCAG+TGG | + | chr3_2:83414730-83414749 | None:intergenic | 45.0% |
| ! | TGGCCTGAAAAATGATTCGG+AGG | + | chr3_2:83414770-83414789 | None:intergenic | 45.0% |
| TACCCATCACACAACCAAAG+AGG | - | chr3_2:83414791-83414810 | Msa0431360:CDS | 45.0% | |
| GATCCTCTTTGGTTGTGTGA+TGG | + | chr3_2:83414797-83414816 | None:intergenic | 45.0% | |
| TATAACCATGGAGGCTGTCA+AGG | + | chr3_2:83414893-83414912 | None:intergenic | 45.0% | |
| CACATCAGGTATAACCATGG+AGG | + | chr3_2:83414902-83414921 | None:intergenic | 45.0% | |
| CTCCATGGTTATACCTGATG+TGG | - | chr3_2:83414900-83414919 | Msa0431360:CDS | 45.0% | |
| AGCCACATCAGGTATAACCA+TGG | + | chr3_2:83414905-83414924 | None:intergenic | 45.0% | |
| GCTGTGCTAACAGCTATACT+TGG | - | chr3_2:83414991-83415010 | Msa0431360:CDS | 45.0% | |
| TGGGGTTTCCAACTTTGATC+CGG | - | chr3_2:83415023-83415042 | Msa0431360:CDS | 45.0% | |
| TGCATCCTTGACAGCCTCCA+TGG | - | chr3_2:83414885-83414904 | Msa0431360:CDS | 55.0% | |
| CCAGCTGCATGAGCCACATC+AGG | + | chr3_2:83414916-83414935 | None:intergenic | 60.0% | |
| CCTGATGTGGCTCATGCAGC+TGG | - | chr3_2:83414913-83414932 | Msa0431360:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3_2 | gene | 83414697 | 83415059 | 83414697 | ID=Msa0431360;Name=Msa0431360 |
| chr3_2 | mRNA | 83414697 | 83415059 | 83414697 | ID=Msa0431360-mRNA-1;Parent=Msa0431360;Name=Msa0431360-mRNA-1;_AED=0.46;_eAED=0.46;_QI=0|-1|0|1|-1|1|1|0|120 |
| chr3_2 | exon | 83414697 | 83415059 | 83414697 | ID=Msa0431360-mRNA-1:exon:23355;Parent=Msa0431360-mRNA-1 |
| chr3_2 | CDS | 83414697 | 83415059 | 83414697 | ID=Msa0431360-mRNA-1:cds;Parent=Msa0431360-mRNA-1 |
| Gene Sequence |
| Protein sequence |
