AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0524820

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0524820 MtrunA17_Chr3g0137981 98.140 215 4 0 1 215 47 261 3.42e-153 425
Msa0524820 MtrunA17_Chr4g0003661 51.149 174 81 2 19 192 11 180 3.00e-51 164
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0524820 AT5G11280.1 72.195 205 54 2 13 215 6 209 2.94e-111 317
Msa0524820 AT1G80200.3 40.933 193 108 4 6 192 25 217 1.99e-41 140
Msa0524820 AT1G80200.2 40.933 193 108 4 6 192 27 219 2.61e-41 140
Msa0524820 AT1G80200.1 41.935 186 102 4 13 192 4 189 2.73e-41 139

Find 78 sgRNAs with CRISPR-Local

Find 234 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATGAAAGTTTAACAATTC+TGG 0.201220 3_4:+78372368 None:intergenic
TACCCTGGTGCTGCTTTAAA+TGG 0.213795 3_4:-78373525 Msa0524820:CDS
TTGGATCACTTCTTGTGATA+TGG 0.253529 3_4:-78372681 Msa0524820:CDS
CCTTTCTTCAAAATTCCTTT+TGG 0.263981 3_4:-78372421 Msa0524820:three_prime_UTR
TGTTCCTGTTGGCACACAAC+TGG 0.311672 3_4:-78373897 Msa0524820:five_prime_UTR
TTCGCAGGATCAGTATTTAC+TGG 0.318077 3_4:+78372628 None:intergenic
TGTAAAGTATGATATGAAAA+AGG 0.337916 3_4:-78372551 Msa0524820:CDS
CCCGGTAGAATGAGCATGTT+TGG 0.342893 3_4:-78372700 Msa0524820:CDS
AGTTGTGTGCCAACAGGAAC+AGG 0.356731 3_4:+78373899 None:intergenic
ATGATTTATCAGGTCCTTGC+AGG 0.367749 3_4:-78372766 Msa0524820:intron
TCCTAGTTGGGATAGTTTGT+TGG 0.385762 3_4:-78373481 Msa0524820:CDS
GACTTTGATGAGCTCTTAAG+AGG 0.392333 3_4:+78372490 None:intergenic
TTTCCTGTTCCACTTGTAAA+GGG 0.393641 3_4:-78373555 Msa0524820:CDS
CTGCCAGGGCGGCCCCTGTC+CGG 0.403387 3_4:-78372525 Msa0524820:CDS
TACAGGGTTACATGCAGATC+TGG 0.408658 3_4:-78373633 Msa0524820:intron
ATCATGGCAAACACCATTGT+TGG 0.415601 3_4:+78372589 None:intergenic
GGAAGCTACTTTGCAGTAGC+AGG 0.416283 3_4:-78372745 Msa0524820:CDS
TGGATCACTTCTTGTGATAT+GGG 0.418628 3_4:-78372680 Msa0524820:CDS
GCAGTAGCAGGAGCACTTGT+TGG 0.425218 3_4:-78372733 Msa0524820:CDS
ATTTCCTGTTCCACTTGTAA+AGG 0.426556 3_4:-78373556 Msa0524820:CDS
GTGATGATGTTACATGTAAA+GGG 0.435849 3_4:+78373598 None:intergenic
TGAGGAAATGTTCCATCCCT+TGG 0.437177 3_4:+78373782 None:intergenic
AGACCCTTTACAAGTGGAAC+AGG 0.438573 3_4:+78373552 None:intergenic
ATCTTCTTCTTCCTTCTCCA+TGG 0.442248 3_4:-78373841 Msa0524820:exon
CTTCCCAGTTGTGTGCCAAC+AGG 0.443594 3_4:+78373893 None:intergenic
CAACATGACTGTTCCTAGTT+GGG 0.458590 3_4:-78373493 Msa0524820:CDS
TGCAGAAGCTTCCTTGATAG+TGG 0.474544 3_4:+78373443 None:intergenic
AAATAGAGAAAATAAGATGA+TGG 0.476204 3_4:-78373928 None:intergenic
TCCAAACATGCTCATTCTAC+CGG 0.484946 3_4:+78372699 None:intergenic
TGTGATGATGTTACATGTAA+AGG 0.485821 3_4:+78373597 None:intergenic
GGACCGGACAGGGGCCGCCC+TGG 0.487426 3_4:+78372522 None:intergenic
AATAGAGAAAATAAGATGAT+GGG 0.490358 3_4:-78373927 None:intergenic
ACAACATGACTGTTCCTAGT+TGG 0.492866 3_4:-78373494 Msa0524820:CDS
CTATGTCTATCTGTTCCAAA+AGG 0.493372 3_4:+78372406 None:intergenic
GTATAATTTGACCACTATCA+AGG 0.493949 3_4:-78373454 Msa0524820:CDS
CTTGGAAGATGATGATGAGT+AGG 0.494468 3_4:+78373742 None:intergenic
AACCGATCTTCAACGCTTGG+AGG 0.495020 3_4:-78373418 Msa0524820:intron
GGTTTAGCAGTGGACCGGAC+AGG 0.498097 3_4:+78372511 None:intergenic
GTAGATGCGTGCTGCGATTG+AGG 0.506076 3_4:+78373764 None:intergenic
AGACAAGAAGGCACATATCA+TGG 0.508797 3_4:+78372573 None:intergenic
GTTTAGCAGTGGACCGGACA+GGG 0.519587 3_4:+78372512 None:intergenic
TCATGGCAAACACCATTGTT+GGG 0.522116 3_4:+78372590 None:intergenic
GGATCACTTCTTGTGATATG+GGG 0.524575 3_4:-78372679 Msa0524820:CDS
GAACAGTCATGTTGTGGATG+AGG 0.528098 3_4:+78373502 None:intergenic
AGTACCTGAAAAGAGGGACT+TGG 0.536129 3_4:+78373724 None:intergenic
CCAAACATGCTCATTCTACC+GGG 0.539556 3_4:+78372700 None:intergenic
TAAGAGGTTTAGCAGTGGAC+CGG 0.544646 3_4:+78372506 None:intergenic
ATCATACTTTACAGACAAGA+AGG 0.545178 3_4:+78372561 None:intergenic
GAAATCATCACCTTCATCCA+AGG 0.545466 3_4:-78373799 Msa0524820:CDS
ATGTTCCATCCCTTGGATGA+AGG 0.546480 3_4:+78373789 None:intergenic
GTTCCTGTTGGCACACAACT+GGG 0.559343 3_4:-78373896 Msa0524820:five_prime_UTR
GCACACAACTGGGAAGAAGT+TGG 0.560545 3_4:-78373886 Msa0524820:five_prime_UTR
AGTAACCGATCTTCAACGCT+TGG 0.561137 3_4:-78373421 Msa0524820:CDS
ACTTCTTGTGATATGGGGAC+TGG 0.561214 3_4:-78372674 Msa0524820:CDS
AGGCCATTTAAAGCAGCACC+AGG 0.563544 3_4:+78373522 None:intergenic
AACTAGGAACAGTCATGTTG+TGG 0.564260 3_4:+78373496 None:intergenic
AACTTGTGTTGCCATGGAGA+AGG 0.565910 3_4:+78373830 None:intergenic
ATATGGGGACTGGTCAAAGA+AGG 0.566921 3_4:-78372664 Msa0524820:CDS
AACCTCCAAGCGTTGAAGAT+CGG 0.570379 3_4:+78373416 None:intergenic
GTTTGGGAGTACCTGAAAAG+AGG 0.572459 3_4:+78373717 None:intergenic
AGATACAACTTGTGTTGCCA+TGG 0.577324 3_4:+78373824 None:intergenic
GATCAGATGCTTCTCGACGA+TGG 0.582609 3_4:+78373861 None:intergenic
TACAAAAGACCCTTTACAAG+TGG 0.585226 3_4:+78373546 None:intergenic
TAGACGTAGACAGCTTTCGC+AGG 0.586449 3_4:+78372613 None:intergenic
CATCACCTTCATCCAAGGGA+TGG 0.592421 3_4:-78373794 Msa0524820:CDS
CTTGTTGGTATTCTGAAACC+CGG 0.595853 3_4:-78372718 Msa0524820:CDS
ATAGAGAAAATAAGATGATG+GGG 0.608587 3_4:-78373926 None:intergenic
TTTGGGAGTACCTGAAAAGA+GGG 0.608777 3_4:+78373718 None:intergenic
TGGGGACACCCTGTTCCTGT+TGG 0.612744 3_4:-78373908 Msa0524820:five_prime_UTR
AAATCATCACCTTCATCCAA+GGG 0.613603 3_4:-78373798 Msa0524820:CDS
TTTAGCAGTGGACCGGACAG+GGG 0.629054 3_4:+78372513 None:intergenic
TCCAACAAACTATCCCAACT+AGG 0.646774 3_4:+78373480 None:intergenic
CTGCAAAGTAGCTTCCTGCA+AGG 0.676389 3_4:+78372752 None:intergenic
GTTGTGTGCCAACAGGAACA+GGG 0.678585 3_4:+78373900 None:intergenic
TGTCTACGTCTACCCAACAA+TGG 0.684219 3_4:-78372602 Msa0524820:CDS
GCTCTTAAGAGGTTTAGCAG+TGG 0.687561 3_4:+78372501 None:intergenic
GGCCATTTAAAGCAGCACCA+GGG 0.713591 3_4:+78373523 None:intergenic
TGATGATGTTACATGTAAAG+GGG 0.715942 3_4:+78373599 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATTTATCATTTTGATTAC+AGG - chr3_4:78372597-78372616 Msa0524820:CDS 15.0%
!!! AATTTATCATTTTGATTACA+GGG - chr3_4:78372598-78372617 Msa0524820:CDS 15.0%
!!! ATATTTTTCAATGATTTATC+AGG - chr3_4:78373471-78373490 Msa0524820:CDS 15.0%
!! TCAATCAATTAATAGAAAAA+TGG + chr3_4:78373907-78373926 None:intergenic 15.0%
!!! AAATTTATCATTTTGATTAC+AGG - chr3_4:78372597-78372616 Msa0524820:CDS 15.0%
!!! AATTTATCATTTTGATTACA+GGG - chr3_4:78372598-78372617 Msa0524820:CDS 15.0%
!!! ATATTTTTCAATGATTTATC+AGG - chr3_4:78373471-78373490 Msa0524820:CDS 15.0%
!! TCAATCAATTAATAGAAAAA+TGG + chr3_4:78373907-78373926 None:intergenic 15.0%
!! AATATGTTAGAATAACTAGA+AGG + chr3_4:78372570-78372589 None:intergenic 20.0%
!!! TTCTTTTTCTGTATTTAGAT+CGG - chr3_4:78372876-78372895 Msa0524820:intron 20.0%
!!! TGTAACATTTTTTGTTGAAA+TGG - chr3_4:78373135-78373154 Msa0524820:intron 20.0%
!!! TGCATAAGCAAATTTTTTTA+AGG - chr3_4:78373219-78373238 Msa0524820:intron 20.0%
!!! TAAGCAAATTTTTTTAAGGA+AGG - chr3_4:78373223-78373242 Msa0524820:intron 20.0%
!! TAAATCATTGAAAAATATCC+AGG + chr3_4:78373469-78373488 None:intergenic 20.0%
!! TGTAAAGTATGATATGAAAA+AGG - chr3_4:78373696-78373715 Msa0524820:intron 20.0%
!! AAATGAAAGTTTAACAATTC+TGG + chr3_4:78373882-78373901 None:intergenic 20.0%
!! AATATGTTAGAATAACTAGA+AGG + chr3_4:78372570-78372589 None:intergenic 20.0%
!!! TTCTTTTTCTGTATTTAGAT+CGG - chr3_4:78372876-78372895 Msa0524820:intron 20.0%
!!! TGTAACATTTTTTGTTGAAA+TGG - chr3_4:78373135-78373154 Msa0524820:intron 20.0%
!!! TGCATAAGCAAATTTTTTTA+AGG - chr3_4:78373219-78373238 Msa0524820:intron 20.0%
!!! TAAGCAAATTTTTTTAAGGA+AGG - chr3_4:78373223-78373242 Msa0524820:intron 20.0%
!! TAAATCATTGAAAAATATCC+AGG + chr3_4:78373469-78373488 None:intergenic 20.0%
!! TGTAAAGTATGATATGAAAA+AGG - chr3_4:78373696-78373715 Msa0524820:intron 20.0%
!! AAATGAAAGTTTAACAATTC+TGG + chr3_4:78373882-78373901 None:intergenic 20.0%
! GGAAGAAATAAAAAGAGTTT+GGG + chr3_4:78372549-78372568 None:intergenic 25.0%
! AGGAAGAAATAAAAAGAGTT+TGG + chr3_4:78372550-78372569 None:intergenic 25.0%
!!! TTTTTTTTTTGGTTCAGACA+TGG - chr3_4:78373095-78373114 Msa0524820:intron 25.0%
! TAGTCATAGCAATTGATTAT+TGG - chr3_4:78373181-78373200 Msa0524820:intron 25.0%
! AAATAGCTGAAATATCCAAA+GGG + chr3_4:78373391-78373410 None:intergenic 25.0%
! AAAATAGCTGAAATATCCAA+AGG + chr3_4:78373392-78373411 None:intergenic 25.0%
! GGAAGAAATAAAAAGAGTTT+GGG + chr3_4:78372549-78372568 None:intergenic 25.0%
! AGGAAGAAATAAAAAGAGTT+TGG + chr3_4:78372550-78372569 None:intergenic 25.0%
!!! TTTTTTTTTTGGTTCAGACA+TGG - chr3_4:78373095-78373114 Msa0524820:intron 25.0%
! TAGTCATAGCAATTGATTAT+TGG - chr3_4:78373181-78373200 Msa0524820:intron 25.0%
! AAATAGCTGAAATATCCAAA+GGG + chr3_4:78373391-78373410 None:intergenic 25.0%
! AAAATAGCTGAAATATCCAA+AGG + chr3_4:78373392-78373411 None:intergenic 25.0%
! TGATGATGTTACATGTAAAG+GGG + chr3_4:78372651-78372670 None:intergenic 30.0%
! GTGATGATGTTACATGTAAA+GGG + chr3_4:78372652-78372671 None:intergenic 30.0%
! TGTGATGATGTTACATGTAA+AGG + chr3_4:78372653-78372672 None:intergenic 30.0%
GTATAATTTGACCACTATCA+AGG - chr3_4:78372793-78372812 Msa0524820:intron 30.0%
AAATAACATGGCATGTCTAT+TGG - chr3_4:78372956-78372975 Msa0524820:intron 30.0%
AAGCAATCATGAAGAACTTA+CGG + chr3_4:78372999-78373018 None:intergenic 30.0%
ATCAATTGCTATGACTATGT+AGG + chr3_4:78373178-78373197 None:intergenic 30.0%
! AGAGTATTCTCATTACTATG+AGG + chr3_4:78373260-78373279 None:intergenic 30.0%
! GCATATTTTATGCGAGTTTT+AGG + chr3_4:78373309-78373328 None:intergenic 30.0%
CAAATAGACAAGAACAACTA+GGG + chr3_4:78373333-78373352 None:intergenic 30.0%
! GTTCTTGTCTATTTGCATTT+TGG - chr3_4:78373338-78373357 Msa0524820:intron 30.0%
ATCATACTTTACAGACAAGA+AGG + chr3_4:78373689-78373708 None:intergenic 30.0%
!! CCAAAAGGAATTTTGAAGAA+AGG + chr3_4:78373829-78373848 None:intergenic 30.0%
! CCTTTCTTCAAAATTCCTTT+TGG - chr3_4:78373826-78373845 Msa0524820:exon 30.0%
! TGATGATGTTACATGTAAAG+GGG + chr3_4:78372651-78372670 None:intergenic 30.0%
! GTGATGATGTTACATGTAAA+GGG + chr3_4:78372652-78372671 None:intergenic 30.0%
! TGTGATGATGTTACATGTAA+AGG + chr3_4:78372653-78372672 None:intergenic 30.0%
GTATAATTTGACCACTATCA+AGG - chr3_4:78372793-78372812 Msa0524820:intron 30.0%
AAATAACATGGCATGTCTAT+TGG - chr3_4:78372956-78372975 Msa0524820:intron 30.0%
AAGCAATCATGAAGAACTTA+CGG + chr3_4:78372999-78373018 None:intergenic 30.0%
ATCAATTGCTATGACTATGT+AGG + chr3_4:78373178-78373197 None:intergenic 30.0%
! AGAGTATTCTCATTACTATG+AGG + chr3_4:78373260-78373279 None:intergenic 30.0%
! GCATATTTTATGCGAGTTTT+AGG + chr3_4:78373309-78373328 None:intergenic 30.0%
CAAATAGACAAGAACAACTA+GGG + chr3_4:78373333-78373352 None:intergenic 30.0%
! GTTCTTGTCTATTTGCATTT+TGG - chr3_4:78373338-78373357 Msa0524820:intron 30.0%
ATCATACTTTACAGACAAGA+AGG + chr3_4:78373689-78373708 None:intergenic 30.0%
!! CCAAAAGGAATTTTGAAGAA+AGG + chr3_4:78373829-78373848 None:intergenic 30.0%
! CCTTTCTTCAAAATTCCTTT+TGG - chr3_4:78373826-78373845 Msa0524820:exon 30.0%
AAATCATCACCTTCATCCAA+GGG - chr3_4:78372449-78372468 Msa0524820:three_prime_UTR 35.0%
ATTTCCTGTTCCACTTGTAA+AGG - chr3_4:78372691-78372710 Msa0524820:CDS 35.0%
TTTCCTGTTCCACTTGTAAA+GGG - chr3_4:78372692-78372711 Msa0524820:CDS 35.0%
TACAAAAGACCCTTTACAAG+TGG + chr3_4:78372704-78372723 None:intergenic 35.0%
GAGAGTTCATGGAAATAACA+TGG - chr3_4:78372944-78372963 Msa0524820:intron 35.0%
GCAAATAGACAAGAACAACT+AGG + chr3_4:78373334-78373353 None:intergenic 35.0%
!! ACTTGTTATAAGGCAGATGA+AGG + chr3_4:78373368-78373387 None:intergenic 35.0%
!! ATCCAAAGGGACTTGTTATA+AGG + chr3_4:78373378-78373397 None:intergenic 35.0%
GTACATAGTCTATTTCCATC+AGG - chr3_4:78373419-78373438 Msa0524820:CDS 35.0%
TTGGATCACTTCTTGTGATA+TGG - chr3_4:78373566-78373585 Msa0524820:CDS 35.0%
TGGATCACTTCTTGTGATAT+GGG - chr3_4:78373567-78373586 Msa0524820:CDS 35.0%
! CTGGTCAAAGAAGGAATTTT+AGG - chr3_4:78373592-78373611 Msa0524820:CDS 35.0%
ATATGAAAAAGGTTACTGCC+AGG - chr3_4:78373707-78373726 Msa0524820:intron 35.0%
TATGAAAAAGGTTACTGCCA+GGG - chr3_4:78373708-78373727 Msa0524820:intron 35.0%
CTATGTCTATCTGTTCCAAA+AGG + chr3_4:78373844-78373863 None:intergenic 35.0%
AAATCATCACCTTCATCCAA+GGG - chr3_4:78372449-78372468 Msa0524820:three_prime_UTR 35.0%
ATTTCCTGTTCCACTTGTAA+AGG - chr3_4:78372691-78372710 Msa0524820:CDS 35.0%
TTTCCTGTTCCACTTGTAAA+GGG - chr3_4:78372692-78372711 Msa0524820:CDS 35.0%
TACAAAAGACCCTTTACAAG+TGG + chr3_4:78372704-78372723 None:intergenic 35.0%
GAGAGTTCATGGAAATAACA+TGG - chr3_4:78372944-78372963 Msa0524820:intron 35.0%
GCAAATAGACAAGAACAACT+AGG + chr3_4:78373334-78373353 None:intergenic 35.0%
!! ACTTGTTATAAGGCAGATGA+AGG + chr3_4:78373368-78373387 None:intergenic 35.0%
!! ATCCAAAGGGACTTGTTATA+AGG + chr3_4:78373378-78373397 None:intergenic 35.0%
GTACATAGTCTATTTCCATC+AGG - chr3_4:78373419-78373438 Msa0524820:CDS 35.0%
TTGGATCACTTCTTGTGATA+TGG - chr3_4:78373566-78373585 Msa0524820:CDS 35.0%
TGGATCACTTCTTGTGATAT+GGG - chr3_4:78373567-78373586 Msa0524820:CDS 35.0%
! CTGGTCAAAGAAGGAATTTT+AGG - chr3_4:78373592-78373611 Msa0524820:CDS 35.0%
ATATGAAAAAGGTTACTGCC+AGG - chr3_4:78373707-78373726 Msa0524820:intron 35.0%
TATGAAAAAGGTTACTGCCA+GGG - chr3_4:78373708-78373727 Msa0524820:intron 35.0%
CTATGTCTATCTGTTCCAAA+AGG + chr3_4:78373844-78373863 None:intergenic 35.0%
ATCTTCTTCTTCCTTCTCCA+TGG - chr3_4:78372406-78372425 Msa0524820:three_prime_UTR 40.0%
AGATACAACTTGTGTTGCCA+TGG + chr3_4:78372426-78372445 None:intergenic 40.0%
GAAATCATCACCTTCATCCA+AGG - chr3_4:78372448-78372467 Msa0524820:three_prime_UTR 40.0%
! CTTGGAAGATGATGATGAGT+AGG + chr3_4:78372508-78372527 None:intergenic 40.0%
!! TTTGGGAGTACCTGAAAAGA+GGG + chr3_4:78372532-78372551 None:intergenic 40.0%
AACTAGGAACAGTCATGTTG+TGG + chr3_4:78372754-78372773 None:intergenic 40.0%
ACAACATGACTGTTCCTAGT+TGG - chr3_4:78372753-78372772 Msa0524820:CDS 40.0%
CAACATGACTGTTCCTAGTT+GGG - chr3_4:78372754-78372773 Msa0524820:CDS 40.0%
! TCCTAGTTGGGATAGTTTGT+TGG - chr3_4:78372766-78372785 Msa0524820:intron 40.0%
TCCAACAAACTATCCCAACT+AGG + chr3_4:78372770-78372789 None:intergenic 40.0%
AGACTGTTTGAGAGAGTTCA+TGG - chr3_4:78372933-78372952 Msa0524820:intron 40.0%
TGCCTTATAACAAGTCCCTT+TGG - chr3_4:78373373-78373392 Msa0524820:intron 40.0%
CTATTTCCATCAGGTCTATG+CGG - chr3_4:78373428-78373447 Msa0524820:CDS 40.0%
TATTTCCATCAGGTCTATGC+GGG - chr3_4:78373429-78373448 Msa0524820:CDS 40.0%
ATGATTTATCAGGTCCTTGC+AGG - chr3_4:78373481-78373500 Msa0524820:CDS 40.0%
!! CTTGTTGGTATTCTGAAACC+CGG - chr3_4:78373529-78373548 Msa0524820:CDS 40.0%
TCCAAACATGCTCATTCTAC+CGG + chr3_4:78373551-78373570 None:intergenic 40.0%
GGATCACTTCTTGTGATATG+GGG - chr3_4:78373568-78373587 Msa0524820:CDS 40.0%
TTCGCAGGATCAGTATTTAC+TGG + chr3_4:78373622-78373641 None:intergenic 40.0%
TCATGGCAAACACCATTGTT+GGG + chr3_4:78373660-78373679 None:intergenic 40.0%
ATCATGGCAAACACCATTGT+TGG + chr3_4:78373661-78373680 None:intergenic 40.0%
! AGACAAGAAGGCACATATCA+TGG + chr3_4:78373677-78373696 None:intergenic 40.0%
GACTTTGATGAGCTCTTAAG+AGG + chr3_4:78373760-78373779 None:intergenic 40.0%
ATCTTCTTCTTCCTTCTCCA+TGG - chr3_4:78372406-78372425 Msa0524820:three_prime_UTR 40.0%
AGATACAACTTGTGTTGCCA+TGG + chr3_4:78372426-78372445 None:intergenic 40.0%
GAAATCATCACCTTCATCCA+AGG - chr3_4:78372448-78372467 Msa0524820:three_prime_UTR 40.0%
! CTTGGAAGATGATGATGAGT+AGG + chr3_4:78372508-78372527 None:intergenic 40.0%
!! TTTGGGAGTACCTGAAAAGA+GGG + chr3_4:78372532-78372551 None:intergenic 40.0%
AACTAGGAACAGTCATGTTG+TGG + chr3_4:78372754-78372773 None:intergenic 40.0%
ACAACATGACTGTTCCTAGT+TGG - chr3_4:78372753-78372772 Msa0524820:CDS 40.0%
CAACATGACTGTTCCTAGTT+GGG - chr3_4:78372754-78372773 Msa0524820:CDS 40.0%
! TCCTAGTTGGGATAGTTTGT+TGG - chr3_4:78372766-78372785 Msa0524820:intron 40.0%
TCCAACAAACTATCCCAACT+AGG + chr3_4:78372770-78372789 None:intergenic 40.0%
AGACTGTTTGAGAGAGTTCA+TGG - chr3_4:78372933-78372952 Msa0524820:intron 40.0%
TGCCTTATAACAAGTCCCTT+TGG - chr3_4:78373373-78373392 Msa0524820:intron 40.0%
CTATTTCCATCAGGTCTATG+CGG - chr3_4:78373428-78373447 Msa0524820:CDS 40.0%
TATTTCCATCAGGTCTATGC+GGG - chr3_4:78373429-78373448 Msa0524820:CDS 40.0%
ATGATTTATCAGGTCCTTGC+AGG - chr3_4:78373481-78373500 Msa0524820:CDS 40.0%
!! CTTGTTGGTATTCTGAAACC+CGG - chr3_4:78373529-78373548 Msa0524820:CDS 40.0%
TCCAAACATGCTCATTCTAC+CGG + chr3_4:78373551-78373570 None:intergenic 40.0%
GGATCACTTCTTGTGATATG+GGG - chr3_4:78373568-78373587 Msa0524820:CDS 40.0%
TTCGCAGGATCAGTATTTAC+TGG + chr3_4:78373622-78373641 None:intergenic 40.0%
TCATGGCAAACACCATTGTT+GGG + chr3_4:78373660-78373679 None:intergenic 40.0%
ATCATGGCAAACACCATTGT+TGG + chr3_4:78373661-78373680 None:intergenic 40.0%
! AGACAAGAAGGCACATATCA+TGG + chr3_4:78373677-78373696 None:intergenic 40.0%
GACTTTGATGAGCTCTTAAG+AGG + chr3_4:78373760-78373779 None:intergenic 40.0%
AACTTGTGTTGCCATGGAGA+AGG + chr3_4:78372420-78372439 None:intergenic 45.0%
ATGTTCCATCCCTTGGATGA+AGG + chr3_4:78372461-78372480 None:intergenic 45.0%
TGAGGAAATGTTCCATCCCT+TGG + chr3_4:78372468-78372487 None:intergenic 45.0%
! TCTTCCAAGTCCCTCTTTTC+AGG - chr3_4:78372519-78372538 Msa0524820:CDS 45.0%
AGTACCTGAAAAGAGGGACT+TGG + chr3_4:78372526-78372545 None:intergenic 45.0%
!! GTTTGGGAGTACCTGAAAAG+AGG + chr3_4:78372533-78372552 None:intergenic 45.0%
TACAGGGTTACATGCAGATC+TGG - chr3_4:78372614-78372633 Msa0524820:CDS 45.0%
AGACCCTTTACAAGTGGAAC+AGG + chr3_4:78372698-78372717 None:intergenic 45.0%
! GTAAAGGGTCTTTTGTACCC+TGG - chr3_4:78372707-78372726 Msa0524820:CDS 45.0%
!! TACCCTGGTGCTGCTTTAAA+TGG - chr3_4:78372722-78372741 Msa0524820:CDS 45.0%
GAACAGTCATGTTGTGGATG+AGG + chr3_4:78372748-78372767 None:intergenic 45.0%
TGCAGAAGCTTCCTTGATAG+TGG + chr3_4:78372807-78372826 None:intergenic 45.0%
AGTAACCGATCTTCAACGCT+TGG - chr3_4:78372826-78372845 Msa0524820:intron 45.0%
!! AACCTCCAAGCGTTGAAGAT+CGG + chr3_4:78372834-78372853 None:intergenic 45.0%
CCAAACATGCTCATTCTACC+GGG + chr3_4:78373550-78373569 None:intergenic 45.0%
ACTTCTTGTGATATGGGGAC+TGG - chr3_4:78373573-78373592 Msa0524820:CDS 45.0%
!! ATATGGGGACTGGTCAAAGA+AGG - chr3_4:78373583-78373602 Msa0524820:CDS 45.0%
TGTCTACGTCTACCCAACAA+TGG - chr3_4:78373645-78373664 Msa0524820:intron 45.0%
TAAGAGGTTTAGCAGTGGAC+CGG + chr3_4:78373744-78373763 None:intergenic 45.0%
GCTCTTAAGAGGTTTAGCAG+TGG + chr3_4:78373749-78373768 None:intergenic 45.0%
AACTTGTGTTGCCATGGAGA+AGG + chr3_4:78372420-78372439 None:intergenic 45.0%
ATGTTCCATCCCTTGGATGA+AGG + chr3_4:78372461-78372480 None:intergenic 45.0%
TGAGGAAATGTTCCATCCCT+TGG + chr3_4:78372468-78372487 None:intergenic 45.0%
! TCTTCCAAGTCCCTCTTTTC+AGG - chr3_4:78372519-78372538 Msa0524820:CDS 45.0%
AGTACCTGAAAAGAGGGACT+TGG + chr3_4:78372526-78372545 None:intergenic 45.0%
!! GTTTGGGAGTACCTGAAAAG+AGG + chr3_4:78372533-78372552 None:intergenic 45.0%
TACAGGGTTACATGCAGATC+TGG - chr3_4:78372614-78372633 Msa0524820:CDS 45.0%
AGACCCTTTACAAGTGGAAC+AGG + chr3_4:78372698-78372717 None:intergenic 45.0%
! GTAAAGGGTCTTTTGTACCC+TGG - chr3_4:78372707-78372726 Msa0524820:CDS 45.0%
!! TACCCTGGTGCTGCTTTAAA+TGG - chr3_4:78372722-78372741 Msa0524820:CDS 45.0%
GAACAGTCATGTTGTGGATG+AGG + chr3_4:78372748-78372767 None:intergenic 45.0%
TGCAGAAGCTTCCTTGATAG+TGG + chr3_4:78372807-78372826 None:intergenic 45.0%
AGTAACCGATCTTCAACGCT+TGG - chr3_4:78372826-78372845 Msa0524820:intron 45.0%
!! AACCTCCAAGCGTTGAAGAT+CGG + chr3_4:78372834-78372853 None:intergenic 45.0%
CCAAACATGCTCATTCTACC+GGG + chr3_4:78373550-78373569 None:intergenic 45.0%
ACTTCTTGTGATATGGGGAC+TGG - chr3_4:78373573-78373592 Msa0524820:CDS 45.0%
!! ATATGGGGACTGGTCAAAGA+AGG - chr3_4:78373583-78373602 Msa0524820:CDS 45.0%
TGTCTACGTCTACCCAACAA+TGG - chr3_4:78373645-78373664 Msa0524820:intron 45.0%
TAAGAGGTTTAGCAGTGGAC+CGG + chr3_4:78373744-78373763 None:intergenic 45.0%
GCTCTTAAGAGGTTTAGCAG+TGG + chr3_4:78373749-78373768 None:intergenic 45.0%
!!! ATTAAGTTTTTTTTTTTTTT+TGG - chr3_4:78373084-78373103 Msa0524820:intron 5.0%
!!! ATTAAGTTTTTTTTTTTTTT+TGG - chr3_4:78373084-78373103 Msa0524820:intron 5.0%
GTTGTGTGCCAACAGGAACA+GGG + chr3_4:78372350-78372369 None:intergenic 50.0%
AGTTGTGTGCCAACAGGAAC+AGG + chr3_4:78372351-78372370 None:intergenic 50.0%
TGTTCCTGTTGGCACACAAC+TGG - chr3_4:78372350-78372369 Msa0524820:three_prime_UTR 50.0%
GTTCCTGTTGGCACACAACT+GGG - chr3_4:78372351-78372370 Msa0524820:three_prime_UTR 50.0%
GCACACAACTGGGAAGAAGT+TGG - chr3_4:78372361-78372380 Msa0524820:three_prime_UTR 50.0%
GATCAGATGCTTCTCGACGA+TGG + chr3_4:78372389-78372408 None:intergenic 50.0%
CATCACCTTCATCCAAGGGA+TGG - chr3_4:78372453-78372472 Msa0524820:three_prime_UTR 50.0%
! GGCCATTTAAAGCAGCACCA+GGG + chr3_4:78372727-78372746 None:intergenic 50.0%
! AGGCCATTTAAAGCAGCACC+AGG + chr3_4:78372728-78372747 None:intergenic 50.0%
AACCGATCTTCAACGCTTGG+AGG - chr3_4:78372829-78372848 Msa0524820:intron 50.0%
! CGGGCACTTTTCAGTTAACC+TGG - chr3_4:78373448-78373467 Msa0524820:CDS 50.0%
CTGCAAAGTAGCTTCCTGCA+AGG + chr3_4:78373498-78373517 None:intergenic 50.0%
! GGAAGCTACTTTGCAGTAGC+AGG - chr3_4:78373502-78373521 Msa0524820:CDS 50.0%
CCCGGTAGAATGAGCATGTT+TGG - chr3_4:78373547-78373566 Msa0524820:CDS 50.0%
TAGACGTAGACAGCTTTCGC+AGG + chr3_4:78373637-78373656 None:intergenic 50.0%
GAAAAAGGTTACTGCCAGGG+CGG - chr3_4:78373711-78373730 Msa0524820:intron 50.0%
GTTGTGTGCCAACAGGAACA+GGG + chr3_4:78372350-78372369 None:intergenic 50.0%
AGTTGTGTGCCAACAGGAAC+AGG + chr3_4:78372351-78372370 None:intergenic 50.0%
TGTTCCTGTTGGCACACAAC+TGG - chr3_4:78372350-78372369 Msa0524820:three_prime_UTR 50.0%
GTTCCTGTTGGCACACAACT+GGG - chr3_4:78372351-78372370 Msa0524820:three_prime_UTR 50.0%
GCACACAACTGGGAAGAAGT+TGG - chr3_4:78372361-78372380 Msa0524820:three_prime_UTR 50.0%
GATCAGATGCTTCTCGACGA+TGG + chr3_4:78372389-78372408 None:intergenic 50.0%
CATCACCTTCATCCAAGGGA+TGG - chr3_4:78372453-78372472 Msa0524820:three_prime_UTR 50.0%
! GGCCATTTAAAGCAGCACCA+GGG + chr3_4:78372727-78372746 None:intergenic 50.0%
! AGGCCATTTAAAGCAGCACC+AGG + chr3_4:78372728-78372747 None:intergenic 50.0%
AACCGATCTTCAACGCTTGG+AGG - chr3_4:78372829-78372848 Msa0524820:intron 50.0%
! CGGGCACTTTTCAGTTAACC+TGG - chr3_4:78373448-78373467 Msa0524820:CDS 50.0%
CTGCAAAGTAGCTTCCTGCA+AGG + chr3_4:78373498-78373517 None:intergenic 50.0%
! GGAAGCTACTTTGCAGTAGC+AGG - chr3_4:78373502-78373521 Msa0524820:CDS 50.0%
CCCGGTAGAATGAGCATGTT+TGG - chr3_4:78373547-78373566 Msa0524820:CDS 50.0%
TAGACGTAGACAGCTTTCGC+AGG + chr3_4:78373637-78373656 None:intergenic 50.0%
GAAAAAGGTTACTGCCAGGG+CGG - chr3_4:78373711-78373730 Msa0524820:intron 50.0%
CTTCCCAGTTGTGTGCCAAC+AGG + chr3_4:78372357-78372376 None:intergenic 55.0%
GTAGATGCGTGCTGCGATTG+AGG + chr3_4:78372486-78372505 None:intergenic 55.0%
AAGTGCCCGCATAGACCTGA+TGG + chr3_4:78373437-78373456 None:intergenic 55.0%
!! GCAGTAGCAGGAGCACTTGT+TGG - chr3_4:78373514-78373533 Msa0524820:CDS 55.0%
TTTAGCAGTGGACCGGACAG+GGG + chr3_4:78373737-78373756 None:intergenic 55.0%
GTTTAGCAGTGGACCGGACA+GGG + chr3_4:78373738-78373757 None:intergenic 55.0%
CTTCCCAGTTGTGTGCCAAC+AGG + chr3_4:78372357-78372376 None:intergenic 55.0%
GTAGATGCGTGCTGCGATTG+AGG + chr3_4:78372486-78372505 None:intergenic 55.0%
AAGTGCCCGCATAGACCTGA+TGG + chr3_4:78373437-78373456 None:intergenic 55.0%
!! GCAGTAGCAGGAGCACTTGT+TGG - chr3_4:78373514-78373533 Msa0524820:CDS 55.0%
TTTAGCAGTGGACCGGACAG+GGG + chr3_4:78373737-78373756 None:intergenic 55.0%
GTTTAGCAGTGGACCGGACA+GGG + chr3_4:78373738-78373757 None:intergenic 55.0%
TGGGGACACCCTGTTCCTGT+TGG - chr3_4:78372339-78372358 Msa0524820:three_prime_UTR 60.0%
GGTTTAGCAGTGGACCGGAC+AGG + chr3_4:78373739-78373758 None:intergenic 60.0%
TGGGGACACCCTGTTCCTGT+TGG - chr3_4:78372339-78372358 Msa0524820:three_prime_UTR 60.0%
GGTTTAGCAGTGGACCGGAC+AGG + chr3_4:78373739-78373758 None:intergenic 60.0%
! CTGCCAGGGCGGCCCCTGTC+CGG - chr3_4:78373722-78373741 Msa0524820:intron 80.0%
! CTGCCAGGGCGGCCCCTGTC+CGG - chr3_4:78373722-78373741 Msa0524820:intron 80.0%
! GGACCGGACAGGGGCCGCCC+TGG + chr3_4:78373728-78373747 None:intergenic 85.0%
! GGACCGGACAGGGGCCGCCC+TGG + chr3_4:78373728-78373747 None:intergenic 85.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 78372326 78373943 78372326 ID=Msa0524820;Name=Msa0524820
chr3_4 mRNA 78372326 78373943 78372326 ID=Msa0524820-mRNA-1;Parent=Msa0524820;Name=Msa0524820-mRNA-1;_AED=0.06;_eAED=0.06;_QI=99|1|1|1|1|1|3|151|215
chr3_4 exon 78372326 78372776 78372326 ID=Msa0524820-mRNA-1:exon:23219;Parent=Msa0524820-mRNA-1
chr3_4 exon 78373419 78373650 78373419 ID=Msa0524820-mRNA-1:exon:23218;Parent=Msa0524820-mRNA-1
chr3_4 exon 78373729 78373943 78373729 ID=Msa0524820-mRNA-1:exon:23217;Parent=Msa0524820-mRNA-1
chr3_4 five_prime_UTR 78373845 78373943 78373845 ID=Msa0524820-mRNA-1:five_prime_utr;Parent=Msa0524820-mRNA-1
chr3_4 CDS 78373729 78373844 78373729 ID=Msa0524820-mRNA-1:cds;Parent=Msa0524820-mRNA-1
chr3_4 CDS 78373419 78373650 78373419 ID=Msa0524820-mRNA-1:cds;Parent=Msa0524820-mRNA-1
chr3_4 CDS 78372477 78372776 78372477 ID=Msa0524820-mRNA-1:cds;Parent=Msa0524820-mRNA-1
chr3_4 three_prime_UTR 78372326 78372476 78372326 ID=Msa0524820-mRNA-1:three_prime_utr;Parent=Msa0524820-mRNA-1
Gene Sequence

>Msa0524820

ATGGCAACACAAGTTGTATCTTCGAAATCATCACCTTCATCCAAGGGATGGAACATTTCCTCAATCGCAGCACGCATCTACCTACTCATCATCATCTTCCAAGTCCCTCTTTTCAGGGTTACATGCAGATCTGGTTTGTGCACAACCCCTTTACATGTAACATCATCACAGTTAGTTGCAAGTGATGTATTTCCTGTTCCACTTGTAAAGGGTCTTTTGTACCCTGGTGCTGCTTTAAATGGCCTCATCCACAACATGACTGTTCCTAGTTGGGATAGTTTGTTGGATTTGTATAATTTGACCACTATCAAGGAAGCTTCTGCAGTAACCGATCTTCAACGCTTGGAGGTCCTTGCAGGAAGCTACTTTGCAGTAGCAGGAGCACTTGTTGGTATTCTGAAACCCGGTAGAATGAGCATGTTTGGATCACTTCTTGTGATATGGGGACTGGTCAAAGAAGGAATTTTAGGAAAGCCAGTAAATACTGATCCTGCGAAAGCTGTCTACGTCTACCCAACAATGGTGTTTGCCATGATATGTGCCTTCTTGTCTGTAAAGTATGATATGAAAAAGGTTACTGCCAGGGCGGCCCCTGTCCGGTCCACTGCTAAACCTCTTAAGAGCTCATCAAAGTCTAAGCTGAAATAA
Protein sequence

>Msa0524820

MATQVVSSKSSPSSKGWNISSIAARIYLLIIIFQVPLFRVTCRSGLCTTPLHVTSSQLVASDVFPVPLVKGLLYPGAALNGLIHNMTVPSWDSLLDLYNLTTIKEASAVTDLQRLEVLAGSYFAVAGALVGILKPGRMSMFGSLLVIWGLVKEGILGKPVNTDPAKAVYVYPTMVFAMICAFLSVKYDMKKVTARAAPVRSTAKPLKSSSKSKLK*