Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1407210 | sp|O65026|SUS_MEDSA | 100.000 | 805 | 0 | 0 | 1 | 805 | 1 | 805 | 0.0 | 1662 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0000220 | Msa1407210 | -0.821085 | 4.870721e-53 | -8.615850e-47 |
| Msa0001460 | Msa1407210 | -0.804050 | 2.601890e-49 | -8.615850e-47 |
| Msa0003470 | Msa1407210 | -0.834859 | 2.348428e-56 | -8.615850e-47 |
| Msa0005160 | Msa1407210 | -0.815137 | 1.078569e-51 | -8.615850e-47 |
| Msa0005690 | Msa1407210 | -0.837352 | 5.467896e-57 | -8.615850e-47 |
| Msa0006050 | Msa1407210 | -0.848032 | 7.937189e-60 | -8.615850e-47 |
| Msa0006700 | Msa1407210 | -0.829295 | 5.577454e-55 | -8.615850e-47 |
| Msa0006820 | Msa1407210 | -0.811011 | 8.666526e-51 | -8.615850e-47 |
| Msa0008850 | Msa1407210 | -0.881412 | 2.452004e-70 | -8.615850e-47 |
| Msa0012460 | Msa1407210 | -0.822570 | 2.207771e-53 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1407210 | MtrunA17_Chr4g0070011 | 99.255 | 805 | 6 | 0 | 1 | 805 | 1 | 805 | 0.0 | 1651 |
| Msa1407210 | MtrunA17_Chr2g0301621 | 89.000 | 800 | 88 | 0 | 6 | 805 | 4 | 803 | 0.0 | 1485 |
| Msa1407210 | MtrunA17_Chr7g0269581 | 71.056 | 805 | 230 | 3 | 1 | 803 | 1 | 804 | 0.0 | 1201 |
| Msa1407210 | MtrunA17_Chr1g0195241 | 71.745 | 768 | 212 | 3 | 37 | 800 | 20 | 786 | 0.0 | 1148 |
| Msa1407210 | MtrunA17_Chr5g0433761 | 54.783 | 805 | 358 | 4 | 3 | 802 | 4 | 807 | 0.0 | 928 |
| Msa1407210 | MtrunA17_Chr3g0109841 | 56.331 | 774 | 332 | 4 | 37 | 805 | 41 | 813 | 0.0 | 926 |
| Msa1407210 | MtrunA17_Chr6g0481131 | 55.569 | 799 | 349 | 4 | 12 | 805 | 15 | 812 | 0.0 | 924 |
| Msa1407210 | MtrunA17_Chr6g0481171 | 58.686 | 685 | 280 | 2 | 123 | 805 | 1 | 684 | 0.0 | 845 |
| Msa1407210 | MtrunA17_Chr6g0481241 | 51.807 | 166 | 58 | 1 | 631 | 796 | 1 | 144 | 1.64e-54 | 188 |
| Msa1407210 | MtrunA17_Chr6g0481261 | 72.000 | 100 | 28 | 0 | 634 | 733 | 4 | 103 | 6.51e-47 | 162 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1407210 | AT3G43190.2 | 83.582 | 804 | 132 | 0 | 2 | 805 | 4 | 807 | 0.0 | 1413 |
| Msa1407210 | AT3G43190.1 | 83.582 | 804 | 132 | 0 | 2 | 805 | 4 | 807 | 0.0 | 1413 |
| Msa1407210 | AT5G20830.3 | 82.544 | 802 | 140 | 0 | 2 | 803 | 4 | 805 | 0.0 | 1378 |
| Msa1407210 | AT5G20830.2 | 82.544 | 802 | 140 | 0 | 2 | 803 | 4 | 805 | 0.0 | 1378 |
| Msa1407210 | AT5G20830.1 | 82.544 | 802 | 140 | 0 | 2 | 803 | 4 | 805 | 0.0 | 1378 |
| Msa1407210 | AT4G02280.1 | 68.408 | 804 | 251 | 2 | 1 | 801 | 1 | 804 | 0.0 | 1174 |
| Msa1407210 | AT5G49190.1 | 67.665 | 801 | 256 | 2 | 8 | 805 | 5 | 805 | 0.0 | 1147 |
| Msa1407210 | AT5G49190.2 | 68.159 | 782 | 246 | 2 | 27 | 805 | 14 | 795 | 0.0 | 1128 |
| Msa1407210 | AT5G49190.3 | 70.190 | 738 | 219 | 1 | 69 | 805 | 2 | 739 | 0.0 | 1107 |
| Msa1407210 | AT1G73370.3 | 55.486 | 802 | 351 | 4 | 6 | 802 | 9 | 809 | 0.0 | 927 |
Find 231 sgRNAs with CRISPR-Local
Find 295 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACATTGTTGCCTCTTTGT+TGG | 0.493208 | tig0023273:-85430 | Msa1407210:CDS |
| AAAGAAGCTCTTCGATTTCA+GGG | 0.464646 | tig0023273:+84848 | None:intergenic |
| AAATAGTTACCTTGAAAGAA+GGG | 0.496710 | tig0023273:+87394 | None:intergenic |
| AAATGAAGAAATGGAGAAAC+CGG | 0.441308 | tig0023273:-83729 | Msa1407210:three_prime_UTR |
| AAGAAGAACTTGTTGATGGA+AGG | 0.539698 | tig0023273:-86948 | Msa1407210:intron |
| AAGATCAGCGGTAAATTGGC+AGG | 0.537258 | tig0023273:+85206 | None:intergenic |
| AAGCTGACTGATGGTGCATT+TGG | 0.442904 | tig0023273:-87213 | Msa1407210:CDS |
| AAGGCTTGAAGCAAAGGGAA+AGG | 0.545090 | tig0023273:-87298 | Msa1407210:CDS |
| AAGTTCAACATTGTATCTCC+AGG | 0.532060 | tig0023273:-84931 | Msa1407210:CDS |
| AATATAGGTTGTTTACATCT+TGG | 0.337487 | tig0023273:-86012 | Msa1407210:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATGAAACCTTAACTGCTAAT+AGG | - | tig0023273:83472-83491 | Msa1407210:three_prime_UTR | 30.0% | |
| TTCATTCCTATTAGCAGTTA+AGG | + | tig0023273:83481-83500 | None:intergenic | 30.0% | |
| TGAATGAAACATGTTCTCTT+AGG | - | tig0023273:84365-84384 | Msa1407210:CDS | 30.0% | |
| GAATGAAACATGTTCTCTTA+GGG | - | tig0023273:84366-84385 | Msa1407210:CDS | 30.0% | |
| TGACAACATTAAAGACCATT+GGG | + | tig0023273:84714-84733 | None:intergenic | 30.0% | |
| ATGACAACATTAAAGACCAT+TGG | + | tig0023273:84715-84734 | None:intergenic | 30.0% | |
| CATTTCATGTTAAACTAACC+TGG | + | tig0023273:84798-84817 | None:intergenic | 30.0% | |
| GTCATAATATAGTACTCTCT+TGG | - | tig0023273:84997-85016 | Msa1407210:CDS | 30.0% | |
| GCTTACTTGTATATTGTGTA+TGG | - | tig0023273:85053-85072 | Msa1407210:intron | 30.0% | |
| TGGATAATTCTCTAATTGGT+TGG | - | tig0023273:85073-85092 | Msa1407210:intron | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0023273 | gene | 83388 | 87536 | 83388 | ID=Msa1407210;Name=Msa1407210 |
| tig0023273 | mRNA | 83388 | 87536 | 83388 | ID=Msa1407210-mRNA-1;Parent=Msa1407210;Name=Msa1407210-mRNA-1;_AED=0.09;_eAED=0.09;_QI=35|1|1|1|1|1|13|385|805 |
| tig0023273 | exon | 83388 | 83802 | 83388 | ID=Msa1407210-mRNA-1:exon:24989;Parent=Msa1407210-mRNA-1 |
| tig0023273 | exon | 83919 | 84057 | 83919 | ID=Msa1407210-mRNA-1:exon:24988;Parent=Msa1407210-mRNA-1 |
| tig0023273 | exon | 84153 | 84716 | 84153 | ID=Msa1407210-mRNA-1:exon:24987;Parent=Msa1407210-mRNA-1 |
| tig0023273 | exon | 84820 | 85044 | 84820 | ID=Msa1407210-mRNA-1:exon:24986;Parent=Msa1407210-mRNA-1 |
| tig0023273 | exon | 85147 | 85313 | 85147 | ID=Msa1407210-mRNA-1:exon:24985;Parent=Msa1407210-mRNA-1 |
| tig0023273 | exon | 85407 | 85523 | 85407 | ID=Msa1407210-mRNA-1:exon:24984;Parent=Msa1407210-mRNA-1 |
| tig0023273 | exon | 85623 | 85796 | 85623 | ID=Msa1407210-mRNA-1:exon:24983;Parent=Msa1407210-mRNA-1 |
| tig0023273 | exon | 85932 | 86027 | 85932 | ID=Msa1407210-mRNA-1:exon:24982;Parent=Msa1407210-mRNA-1 |
| tig0023273 | exon | 86126 | 86461 | 86126 | ID=Msa1407210-mRNA-1:exon:24981;Parent=Msa1407210-mRNA-1 |
| tig0023273 | exon | 86669 | 86861 | 86669 | ID=Msa1407210-mRNA-1:exon:24980;Parent=Msa1407210-mRNA-1 |
| tig0023273 | exon | 86949 | 87100 | 86949 | ID=Msa1407210-mRNA-1:exon:24979;Parent=Msa1407210-mRNA-1 |
| tig0023273 | exon | 87191 | 87317 | 87191 | ID=Msa1407210-mRNA-1:exon:24978;Parent=Msa1407210-mRNA-1 |
| tig0023273 | exon | 87404 | 87536 | 87404 | ID=Msa1407210-mRNA-1:exon:24977;Parent=Msa1407210-mRNA-1 |
| tig0023273 | five_prime_UTR | 87502 | 87536 | 87502 | ID=Msa1407210-mRNA-1:five_prime_utr;Parent=Msa1407210-mRNA-1 |
| tig0023273 | CDS | 87404 | 87501 | 87404 | ID=Msa1407210-mRNA-1:cds;Parent=Msa1407210-mRNA-1 |
| tig0023273 | CDS | 87191 | 87317 | 87191 | ID=Msa1407210-mRNA-1:cds;Parent=Msa1407210-mRNA-1 |
| tig0023273 | CDS | 86949 | 87100 | 86949 | ID=Msa1407210-mRNA-1:cds;Parent=Msa1407210-mRNA-1 |
| tig0023273 | CDS | 86669 | 86861 | 86669 | ID=Msa1407210-mRNA-1:cds;Parent=Msa1407210-mRNA-1 |
| tig0023273 | CDS | 86126 | 86461 | 86126 | ID=Msa1407210-mRNA-1:cds;Parent=Msa1407210-mRNA-1 |
| tig0023273 | CDS | 85932 | 86027 | 85932 | ID=Msa1407210-mRNA-1:cds;Parent=Msa1407210-mRNA-1 |
| tig0023273 | CDS | 85623 | 85796 | 85623 | ID=Msa1407210-mRNA-1:cds;Parent=Msa1407210-mRNA-1 |
| tig0023273 | CDS | 85407 | 85523 | 85407 | ID=Msa1407210-mRNA-1:cds;Parent=Msa1407210-mRNA-1 |
| tig0023273 | CDS | 85147 | 85313 | 85147 | ID=Msa1407210-mRNA-1:cds;Parent=Msa1407210-mRNA-1 |
| tig0023273 | CDS | 84820 | 85044 | 84820 | ID=Msa1407210-mRNA-1:cds;Parent=Msa1407210-mRNA-1 |
| tig0023273 | CDS | 84153 | 84716 | 84153 | ID=Msa1407210-mRNA-1:cds;Parent=Msa1407210-mRNA-1 |
| tig0023273 | CDS | 83919 | 84057 | 83919 | ID=Msa1407210-mRNA-1:cds;Parent=Msa1407210-mRNA-1 |
| tig0023273 | CDS | 83773 | 83802 | 83773 | ID=Msa1407210-mRNA-1:cds;Parent=Msa1407210-mRNA-1 |
| tig0023273 | three_prime_UTR | 83388 | 83772 | 83388 | ID=Msa1407210-mRNA-1:three_prime_utr;Parent=Msa1407210-mRNA-1 |
| Gene Sequence |
>Msa1407210 ATGGCTACAGAACGTTTGACTCGTGTTCATAGTCTCAAGGAGAGACTTGATGAAACCTTAACTGCTAATAGGAATGAAATTTTGGCCCTTCTTTCAAGGCTTGAAGCAAAGGGAAAGGGAATTTTGCAACACCATCAAGTGATTGCTGAGTTTGAGGAAATTCCTGAAGAGAGTAGACAGAAGCTGACTGATGGTGCATTTGGTGAAGTTTTGAGATCCACACAGGAAGCAATAGTTTTGCCACCATGGGTTGCACTTGCTGTTCGTCCAAGGCCAGGTATTTGGGAGTATCTGAGAGTGAATGTGCATGCTCTTGTTGTTGAAAATTTGCAACCTGCTGAGTTTCTCAAATTCAAAGAAGAACTTGTTGATGGAAGTGCTAATGGAAACTTTGTGCTTGAGTTGGACTTTGAACCATTTACTGCATCTTTCCCTCGTCCTACTCTCAACAAGTCAATTGGAAATGGTGTGCACTTCCTTAATCGCCATCTTTCTGCTAAACTCTTCCATGACAAGGAGAGTTTACATCCACTTTTGGAATTTCTCAGACTTCACAGCTACAAGGGAAAGACATTGATGTTGAATGACAGAATTCAAAACCCCGATTCTCTTCAACATGTTCTGAGGAAAGCTGAAGAGTATCTAAGCACAATTGATCCTGAAACACCATACTCAGAATTTGAACACAGGTTCCAGGAGATTGGTTTGGAGAGAGGCTGGGGAGACACCGCAGAGCGCGTCCTCGAGTCTATCCAACTTCTCTTGGATCTTCTAGAGGCTCCTGACCCTTGCACCCTTGAGTCTTTCCTTGATAGAATCCCAATGGTCTTTAATGTTGTCATCCTTTCTCCTCATGGTTACTTTGCTCAAGATGATGTCTTGGGATACCCTGATACTGGAGGCCAGGTTGTTTACATCTTGGATCAAGTTCGTGCCTTGGAGAGTGAGATGCTCAGTCGCATTAAGAAACAAGGCTTGGATATCATCCCTCGCATTCTCATTATCACTCGTCTTCTCCCCGATGCAGTCGGAACTACCTGTGGCCAACGGCTTGAAAAGGTCTATGGAACTGAGCATTGCCACATTCTTCGAGTTCCCTTCAGAGATGAGAAGGGAATTGTTCGCAAGTGGATCTCACGATTTGAAGTCTGGCCATATCTAGAAACTTACACTGAGGATGTTGCTCACGAGCTTGCCAAAGAGTTGCAAAGCAAACCAGATCTGATTGTTGGAAACTACAGTGATGGAAACATTGTTGCCTCTTTGTTGGCACATAAATTAGGCGTCACTCAGTGTACCATTGCTCATGCACTCGAGAAGACTAAGTACCCCGAATCCGACATTTACTGGAAAAAATTCGAAGAGAAGTATCACTTCTCCTGCCAATTTACCGCTGATCTTTTCGCAATGAACCACACAGATTTCATCATCACTAGTACCTTCCAAGAGATTGCTGGAAGCAAGGATAAGGTTGGACAGTATGAGAGTCACACTGCCTTTACTCTTCCAGGACTCTACCGTGTCGTGCACGGTATTGATGTCTTTGATCCAAAGTTCAACATTGTATCTCCAGGAGCTGATCAGACCATTTACTTCCCTTACACTGAAACTAGCCGCCGATTGACATCCTTCTACCCTGAAATCGAAGAGCTTCTTTACAGCTCAGTTGAGAATGAAGAGCACATATGTGTGCTGAAGGACCGCAACAAGCCAATTATCTTCACCATGGCAAGGTTGGACCGTGTGAAGAACATTACAGGACTTGTGGAGTGGTACGGCAAGAATGCCAAGCTTCGTGAGTTGGTGAACCTTGTTGTTGTGGCCGGAGACAGGAGGAAGGAGTCAAAGGACTTGGAAGAGATAGCTGAGATGAAGAAGATGTATGGCCTAATTGAGACCTACAAGTTGAATGGCCAATTCAGATGGATTTCCTCTCAGATGAACCGTGTCAGAAACGGAGAGCTGTACCGTGTGATTTGTGACACCAAGGGAGCTTTCGTGCAACCTGCCGTGTACGAAGCTTTCGGTTTGACAGTTGTTGAGGCCATGGCTACTGGATTGCCAACATTTGCAACTCTTAATGGTGGCCCTGCTGAGATCATTGTCCATGGCAAATCTGGATTCCACATTGATCCTTACCATGGTGACCGCGCTGCTGATCTCCTTGTTGAATTCTTTGAGAAGGTCAAAGCTGATCCATCTCACTGGGACAAGATCTCTCAAGGTGGTCTCCAACGTATTGAAGAGAAGTATACATGGACAATTTACTCTCAGAGGCTTCTTACACTCACTGGTGTCTATGGCTTCTGGAAGCATGTGTCTAACCTCGACCGTCTTGAGAGCCGCCGCTATCTTGAGATGTTCTATGCTCTCAAGTACCGCAAATTGGCTGAGTCTGTGCCCCTAGCTGTTGAGTAA |
| Protein sequence |
>Msa1407210 MATERLTRVHSLKERLDETLTANRNEILALLSRLEAKGKGILQHHQVIAEFEEIPEESRQKLTDGAFGEVLRSTQEAIVLPPWVALAVRPRPGIWEYLRVNVHALVVENLQPAEFLKFKEELVDGSANGNFVLELDFEPFTASFPRPTLNKSIGNGVHFLNRHLSAKLFHDKESLHPLLEFLRLHSYKGKTLMLNDRIQNPDSLQHVLRKAEEYLSTIDPETPYSEFEHRFQEIGLERGWGDTAERVLESIQLLLDLLEAPDPCTLESFLDRIPMVFNVVILSPHGYFAQDDVLGYPDTGGQVVYILDQVRALESEMLSRIKKQGLDIIPRILIITRLLPDAVGTTCGQRLEKVYGTEHCHILRVPFRDEKGIVRKWISRFEVWPYLETYTEDVAHELAKELQSKPDLIVGNYSDGNIVASLLAHKLGVTQCTIAHALEKTKYPESDIYWKKFEEKYHFSCQFTADLFAMNHTDFIITSTFQEIAGSKDKVGQYESHTAFTLPGLYRVVHGIDVFDPKFNIVSPGADQTIYFPYTETSRRLTSFYPEIEELLYSSVENEEHICVLKDRNKPIIFTMARLDRVKNITGLVEWYGKNAKLRELVNLVVVAGDRRKESKDLEEIAEMKKMYGLIETYKLNGQFRWISSQMNRVRNGELYRVICDTKGAFVQPAVYEAFGLTVVEAMATGLPTFATLNGGPAEIIVHGKSGFHIDPYHGDRAADLLVEFFEKVKADPSHWDKISQGGLQRIEEKYTWTIYSQRLLTLTGVYGFWKHVSNLDRLESRRYLEMFYALKYRKLAESVPLAVE* |
