AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0553090

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0553090 MtrunA17_Chr4g0038671 98.527 543 8 0 1 543 1 543 0.0 1113
Msa0553090 MtrunA17_Chr3g0143671 75.655 534 129 1 11 543 11 544 0.0 857
Msa0553090 MtrunA17_Chr1g0157341 76.199 542 128 1 3 543 2 543 0.0 845
Msa0553090 MtrunA17_Chr4g0024861 72.287 516 142 1 25 539 22 537 0.0 797
Msa0553090 MtrunA17_Chr2g0322441 73.981 515 133 1 25 538 25 539 0.0 788
Msa0553090 MtrunA17_Chr4g0075071 69.541 545 164 2 1 543 1 545 0.0 763
Msa0553090 MtrunA17_Chr4g0024641 70.852 446 128 2 92 536 13 457 0.0 665
Msa0553090 MtrunA17_Chr4g0024641 67.470 83 27 0 458 540 875 957 6.80e-29 122
Msa0553090 MtrunA17_Chr4g0024641 68.182 88 26 2 451 536 804 891 1.53e-28 120
Msa0553090 MtrunA17_Chr4g0024641 69.620 79 24 0 458 536 503 581 4.16e-28 119
Msa0553090 MtrunA17_Chr4g0024641 69.620 79 24 0 458 536 565 643 4.16e-28 119
Msa0553090 MtrunA17_Chr4g0024641 69.620 79 24 0 458 536 627 705 4.16e-28 119
Msa0553090 MtrunA17_Chr4g0024641 69.620 79 24 0 458 536 689 767 4.16e-28 119
Msa0553090 MtrunA17_Chr4g0024641 68.354 79 25 0 458 536 441 519 4.22e-27 116
Msa0553090 MtrunA17_Chr4g0024641 68.354 79 25 0 458 536 751 829 4.45e-27 116
Msa0553090 MtrunA17_Chr8g0374271 60.078 516 204 2 25 539 26 540 0.0 652
Msa0553090 MtrunA17_Chr5g0402831 59.653 518 206 2 22 539 23 537 0.0 630
Msa0553090 MtrunA17_Chr8g0374261 56.538 520 224 2 17 535 16 534 0.0 626
Msa0553090 MtrunA17_Chr4g0043251 54.972 533 230 5 10 533 7 538 0.0 582
Msa0553090 MtrunA17_Chr4g0043261 52.952 525 241 4 14 533 9 532 0.0 556
Msa0553090 MtrunA17_Chr3g0121151 52.222 540 246 6 5 533 39 577 0.0 553
Msa0553090 MtrunA17_Chr3g0121161 51.852 540 248 6 5 533 39 577 0.0 550
Msa0553090 MtrunA17_Chr3g0121171 52.037 540 247 6 5 533 574 1112 1.24e-177 552
Msa0553090 MtrunA17_Chr3g0121171 52.037 540 247 6 5 533 1149 1687 2.49e-177 551
Msa0553090 MtrunA17_Chr3g0121171 53.462 520 232 5 23 533 19 537 2.82e-176 548
Msa0553090 MtrunA17_Chr3g0121171 50.469 533 243 7 12 533 1731 2253 1.56e-165 519
Msa0553090 MtrunA17_Chr4g0056511 49.817 546 249 8 13 534 7 551 2.25e-173 502
Msa0553090 MtrunA17_Chr3g0079321 48.463 553 254 8 11 533 1 552 8.11e-173 501
Msa0553090 MtrunA17_Chr7g0234061 50.190 526 241 8 27 533 26 549 8.95e-169 491
Msa0553090 MtrunA17_Chr1g0197301 47.576 330 170 3 207 533 1 330 1.70e-96 296
Msa0553090 MtrunA17_Chr4g0075781 51.442 208 57 3 134 338 1 167 1.43e-61 200
Msa0553090 MtrunA17_Chr5g0404361 29.668 573 350 16 1 532 51 611 4.89e-61 211
Msa0553090 MtrunA17_Chr5g0404341 29.754 568 338 18 10 532 15 566 8.85e-60 207
Msa0553090 MtrunA17_Chr4g0005921 27.899 552 364 12 13 532 8 557 3.10e-54 192
Msa0553090 MtrunA17_Chr5g0429511 27.240 558 363 14 10 532 9 558 5.33e-51 183
Msa0553090 MtrunA17_Chr5g0436461 27.437 554 352 15 21 532 9 554 6.95e-51 183
Msa0553090 MtrunA17_Chr3g0104061 27.477 535 352 12 30 532 26 556 4.17e-50 181
Msa0553090 MtrunA17_Chr3g0118321 27.103 535 316 18 53 533 49 563 3.28e-48 176
Msa0553090 MtrunA17_Chr8g0382691 26.186 569 339 18 25 537 29 572 3.97e-44 164
Msa0553090 MtrunA17_Chr3g0118331 25.946 555 343 15 30 533 25 562 6.83e-44 164
Msa0553090 MtrunA17_Chr5g0431581 28.471 569 345 21 10 532 8 560 7.23e-44 164
Msa0553090 MtrunA17_Chr1g0197311 38.627 233 102 4 19 211 12 243 1.63e-43 155
Msa0553090 MtrunA17_Chr7g0237491 27.440 543 331 20 3 492 8 540 1.31e-40 155
Msa0553090 MtrunA17_Chr7g0237441 25.235 531 344 16 10 492 15 540 1.67e-40 154
Msa0553090 MtrunA17_Chr3g0114211 26.372 565 366 18 10 532 14 570 7.35e-40 152
Msa0553090 MtrunA17_Chr8g0346581 24.906 530 355 12 30 520 34 559 1.70e-39 151
Msa0553090 MtrunA17_Chr7g0238351 26.930 531 336 17 10 492 15 541 4.68e-38 147
Msa0553090 MtrunA17_Chr7g0240991 27.444 532 331 21 9 492 7 531 1.61e-35 140
Msa0553090 MtrunA17_Chr2g0278811 24.365 591 370 15 14 532 22 607 3.23e-35 139
Msa0553090 MtrunA17_Chr7g0238941 24.339 567 378 16 13 532 16 578 3.68e-35 139
Msa0553090 MtrunA17_Chr5g0437681 24.645 564 374 16 16 532 8 567 4.92e-35 139
Msa0553090 MtrunA17_Chr7g0238981 26.367 512 323 17 30 492 47 553 2.81e-34 137
Msa0553090 MtrunA17_Chr4g0031901 25.147 509 340 16 30 498 44 551 4.53e-33 133
Msa0553090 MtrunA17_Chr7g0240971 26.825 548 322 19 10 497 7 535 4.13e-32 130
Msa0553090 MtrunA17_Chr4g0008521 25.889 506 317 16 44 498 40 538 5.68e-31 127
Msa0553090 MtrunA17_Chr4g0024651 80.645 62 12 0 25 86 22 83 7.61e-30 112
Msa0553090 MtrunA17_Chr3g0118351 23.167 341 210 9 233 533 6 334 9.10e-21 94.0
Msa0553090 MtrunA17_Chr3g0133871 27.619 210 144 4 13 215 10 218 3.08e-18 84.0
Msa0553090 MtrunA17_Chr3g0118341 31.285 179 109 8 8 177 1 174 4.41e-17 80.1
Msa0553090 MtrunA17_Chr3g0133861 26.623 308 173 12 226 492 5 300 1.19e-11 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0553090 AT1G76160.1 75.564 532 130 0 12 543 10 541 0.0 862
Msa0553090 AT1G41830.1 74.109 533 137 1 12 543 10 542 0.0 846
Msa0553090 AT4G38420.1 71.695 537 145 3 13 542 13 549 0.0 795
Msa0553090 AT1G21850.1 69.630 540 160 2 1 540 3 538 0.0 792
Msa0553090 AT1G21860.1 68.972 535 162 2 1 535 3 533 0.0 785
Msa0553090 AT4G22010.1 70.937 523 150 2 23 543 19 541 0.0 777
Msa0553090 AT4G28090.1 64.474 532 180 6 13 538 12 540 0.0 711
Msa0553090 AT4G38420.3 71.429 469 127 3 13 474 13 481 0.0 682
Msa0553090 AT5G66920.1 58.687 518 213 1 22 539 29 545 0.0 646
Msa0553090 AT4G38420.2 71.719 442 118 3 13 447 13 454 0.0 643
Msa0553090 AT4G37160.1 53.761 545 243 3 4 543 1 541 0.0 587
Msa0553090 AT1G55570.1 53.775 543 235 5 11 538 5 546 0.0 585
Msa0553090 AT3G13400.2 54.749 537 233 4 10 537 7 542 0.0 583
Msa0553090 AT3G13400.1 54.749 537 233 4 10 537 7 542 0.0 583
Msa0553090 AT1G55560.1 53.050 541 244 4 6 537 2 541 0.0 574
Msa0553090 AT2G23630.2 54.023 522 239 1 15 535 19 540 0.0 569
Msa0553090 AT2G23630.1 54.023 522 239 1 15 535 14 535 0.0 569
Msa0553090 AT3G13390.1 55.556 522 221 4 26 537 24 544 0.0 568
Msa0553090 AT5G48450.1 49.360 547 261 10 1 534 1 544 0.0 528
Msa0553090 AT5G48450.2 49.360 547 261 10 1 534 1 544 0.0 527
Msa0553090 AT1G75790.1 49.630 540 259 5 8 535 6 544 1.16e-180 520
Msa0553090 AT4G12420.1 49.640 556 256 9 8 542 4 556 3.92e-180 520
Msa0553090 AT4G12420.2 49.640 556 256 9 8 542 4 556 3.92e-180 520
Msa0553090 AT4G25240.2 49.548 553 254 9 4 533 1 551 4.75e-178 514
Msa0553090 AT4G25240.1 49.548 553 254 9 4 533 1 551 4.75e-178 514
Msa0553090 AT5G51480.1 48.346 544 257 9 19 539 17 559 8.46e-178 514
Msa0553090 AT4G37160.2 54.120 449 201 2 4 447 1 449 3.56e-169 487
Msa0553090 AT2G38080.1 28.648 562 351 15 10 532 8 558 6.39e-58 202
Msa0553090 AT5G58910.2 28.000 575 356 15 8 532 1 567 2.65e-56 198
Msa0553090 AT5G58910.3 29.524 525 319 14 51 532 50 566 1.65e-55 196
Msa0553090 AT4G39830.1 27.241 580 352 17 11 535 16 580 4.32e-53 190
Msa0553090 AT5G58910.1 29.143 525 314 15 51 532 14 523 2.78e-51 184
Msa0553090 AT5G21105.1 26.808 567 334 18 25 533 33 576 5.79e-50 181
Msa0553090 AT5G03260.1 27.128 564 365 15 8 532 1 557 2.13e-49 179
Msa0553090 AT5G01190.1 26.620 571 364 14 4 532 1 558 1.03e-48 177
Msa0553090 AT5G21105.3 27.553 519 300 17 30 490 38 538 9.79e-48 174
Msa0553090 AT5G01190.2 27.481 524 327 13 51 532 32 544 1.92e-46 171
Msa0553090 AT5G21100.1 26.040 553 337 16 34 533 28 561 5.29e-46 170
Msa0553090 AT2G30210.1 28.229 542 321 18 6 492 2 530 1.45e-43 163
Msa0553090 AT5G05390.1 26.643 563 363 18 13 532 10 565 9.54e-41 155
Msa0553090 AT2G46570.1 24.675 539 356 15 30 526 32 562 3.59e-39 151
Msa0553090 AT4G39830.3 26.939 490 290 16 98 534 8 482 7.47e-39 149
Msa0553090 AT4G39830.2 26.939 490 290 16 98 534 10 484 8.08e-39 149
Msa0553090 AT5G01040.1 27.514 527 325 15 10 492 13 526 1.68e-38 149
Msa0553090 AT5G09360.1 26.625 477 311 12 51 492 57 529 1.91e-38 149
Msa0553090 AT5G48100.1 26.931 505 312 17 51 507 43 538 3.30e-37 145
Msa0553090 AT1G18140.1 24.955 557 350 18 30 531 29 572 7.83e-37 144
Msa0553090 AT3G09220.1 26.257 537 341 17 1 492 1 527 2.00e-36 143
Msa0553090 AT5G01050.1 27.239 547 329 17 10 505 13 541 2.67e-36 143
Msa0553090 AT2G29130.1 26.095 525 345 17 10 492 10 533 5.18e-36 142
Msa0553090 AT5G07130.1 26.132 574 367 18 9 532 3 569 5.59e-36 142
Msa0553090 AT2G40370.1 24.315 584 383 15 4 532 1 580 1.40e-35 140
Msa0553090 AT4G39830.4 26.327 490 280 16 98 534 10 471 7.95e-35 137
Msa0553090 AT5G21105.2 27.177 379 225 13 184 533 29 385 1.00e-31 127
Msa0553090 AT2G46570.2 24.481 482 314 15 87 526 4 477 2.15e-30 124
Msa0553090 AT5G60020.1 28.028 289 192 6 8 285 3 286 1.12e-26 114
Msa0553090 AT5G60020.2 28.028 289 192 6 8 285 3 286 1.46e-26 114
Msa0553090 AT3G09220.2 26.328 433 271 14 103 492 3 430 1.62e-26 113
Msa0553090 AT5G60020.3 29.675 246 157 6 51 285 14 254 4.63e-25 109
Msa0553090 AT5G07130.2 25.918 490 306 18 93 532 2 484 1.45e-24 107
Msa0553090 AT5G07130.3 25.918 490 306 18 93 532 2 484 1.45e-24 107

Find 163 sgRNAs with CRISPR-Local

Find 269 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACTGAAGTTTCCAATTATT+TGG 0.084217 4_1:-49873522 Msa0553090:three_prime_UTR
TCTTACCAAGATGGAGTTTA+TGG 0.139950 4_1:-49875982 Msa0553090:CDS
CTGAGGAAAGTTCTAGATTT+TGG 0.142759 4_1:-49875536 Msa0553090:CDS
CACAAGGCAGCTGGTGGTTT+TGG 0.145468 4_1:-49875862 Msa0553090:CDS
ACATTGATGGATACTCATTT+TGG 0.174475 4_1:-49874490 Msa0553090:CDS
GTTATTCAAATCCAAATAAT+TGG 0.182004 4_1:+49873511 None:intergenic
CTTCAACTCGTTTCACTAAT+AGG 0.190901 4_1:-49875138 Msa0553090:CDS
TTAGTAACAGAGTAAATTTC+TGG 0.213058 4_1:+49877356 None:intergenic
CAACAACCTAGTTATATATT+AGG 0.223346 4_1:-49873795 Msa0553090:three_prime_UTR
AACTCACAACAACCTCTTTC+AGG 0.244426 4_1:-49875082 Msa0553090:CDS
GATCTACCAGAAGGGGTATC+TGG 0.247730 4_1:+49874605 None:intergenic
GGAATGTGAGGAGTGAATTT+TGG 0.258017 4_1:-49873999 Msa0553090:CDS
TCAAGGCCACATCATGAAAT+TGG 0.262122 4_1:-49875281 Msa0553090:CDS
TGGAGTTGGACTATTACTTA+TGG 0.267372 4_1:+49877684 None:intergenic
CCACAAAGAAGAGCATTCTT+TGG 0.269382 4_1:+49873898 None:intergenic
CTTATCAATGGTCAATTTCC+TGG 0.281749 4_1:-49877380 Msa0553090:CDS
CCAAAGAATGCTCTTCTTTG+TGG 0.292466 4_1:-49873898 Msa0553090:CDS
TTCAAACTTCATTCTCATAA+TGG 0.300039 4_1:+49873850 None:intergenic
AGAAGAGCATTCTTTGGAAT+TGG 0.303850 4_1:+49873904 None:intergenic
TTTGACTGGACTATTACTTA+TGG 0.306131 4_1:-49877534 Msa0553090:CDS
GTGTAATCATTAGCTGGTTC+AGG 0.306591 4_1:+49875796 None:intergenic
TTCAACTCGTTTCACTAATA+GGG 0.306828 4_1:-49875137 Msa0553090:CDS
TTGTATTGGTTACGACTATT+AGG 0.313314 4_1:+49874225 None:intergenic
GTGGTTGGTCCACCGGGAAT+GGG 0.322258 4_1:+49875058 None:intergenic
CTTCAAGTCAAGGATCAAAT+TGG 0.327842 4_1:-49875916 Msa0553090:CDS
ATTCAGATGCTGGATCAATT+AGG 0.328006 4_1:-49873936 Msa0553090:CDS
AGTGGTTGGTCCACCGGGAA+TGG 0.331050 4_1:+49875057 None:intergenic
TACAACATACACTCAACTTT+AGG 0.338339 4_1:-49875306 Msa0553090:CDS
TGGTATGATCCTTGTGGATT+TGG 0.339764 4_1:+49874797 None:intergenic
GTCATGGACTGCAATTTATA+TGG 0.346150 4_1:-49874043 Msa0553090:CDS
GTCCAGTCAAAGAATCTATA+AGG 0.347743 4_1:+49877546 None:intergenic
CCTCTTTCAGGCCCCATTCC+CGG 0.356165 4_1:-49875070 Msa0553090:CDS
AACTTTGGACGATACTTTCA+TGG 0.356230 4_1:+49874516 None:intergenic
CAGCATCTCTTAGGTTGTAT+TGG 0.360857 4_1:+49874211 None:intergenic
GGAGTTGGACTATTACTTAT+GGG 0.371910 4_1:+49877685 None:intergenic
GATCCTTGTGGATTTGGCCT+TGG 0.375319 4_1:+49874803 None:intergenic
TTCAGATGCTGGATCAATTA+GGG 0.377202 4_1:-49873935 Msa0553090:CDS
GATCCATCAAAATCCTTAGT+AGG 0.384492 4_1:-49875840 Msa0553090:CDS
AGCATCCCAGATACCCCTTC+TGG 0.390553 4_1:-49874611 Msa0553090:CDS
AAACAGCAAAAGAAATGGTT+TGG 0.392793 4_1:+49877664 None:intergenic
ATGAACATCTATTGAGTCAT+AGG 0.395960 4_1:+49875225 None:intergenic
CTAATATATAACTAGGTTGT+TGG 0.397045 4_1:+49873796 None:intergenic
AACAACCTAGTTATATATTA+GGG 0.398056 4_1:-49873794 Msa0553090:three_prime_UTR
CTTATCAATGGTCGTGCAAA+TGG 0.412168 4_1:-49875485 Msa0553090:CDS
CTAGCTGATTACTTCAAGAT+TGG 0.412868 4_1:-49874653 Msa0553090:CDS
ACACATTTCTCATCGGTGAT+TGG 0.416274 4_1:-49875777 Msa0553090:CDS
CATGATAAAAGAAATGGTTC+TGG 0.417085 4_1:+49877299 None:intergenic
TGTTACCATGATAAAAGAAA+TGG 0.418474 4_1:+49877293 None:intergenic
ATCCTTATAGATTCTTTGAC+TGG 0.423381 4_1:-49877548 Msa0553090:CDS
GTCCAAAGTTGGCACATTGA+TGG 0.424178 4_1:-49874503 Msa0553090:CDS
ACACTCAACTTTAGGATTCA+AGG 0.438553 4_1:-49875298 Msa0553090:CDS
CTTGCTTTCCACAAGGCAGC+TGG 0.440721 4_1:-49875871 Msa0553090:CDS
ATCCATCAATGTGCCAACTT+TGG 0.444509 4_1:+49874501 None:intergenic
CAGGAATCCTTGGCCTACTA+AGG 0.445461 4_1:+49875827 None:intergenic
TCCTAGACACAGCATCTCTT+AGG 0.448380 4_1:+49874202 None:intergenic
TGGTTAACATTGTGTGGTGT+AGG 0.449159 4_1:+49873816 None:intergenic
ACAGACGAGTTTGCAAAGAA+TGG 0.452707 4_1:+49873759 None:intergenic
ATGAAATTGGTTGAAGTTGA+GGG 0.453531 4_1:-49875268 Msa0553090:CDS
ACCACATGTCCCATCCCTCC+TGG 0.460131 4_1:-49875958 Msa0553090:CDS
ACCTGATGGATCTAGCTTGT+TGG 0.463194 4_1:+49875017 None:intergenic
ATCTGAGTGGTTGGTCCACC+GGG 0.463296 4_1:+49875052 None:intergenic
CTTCTTTGTGGCAAAGCAGT+GGG 0.465661 4_1:-49873886 Msa0553090:CDS
GCTGGTTCAGGGAAAGGAAC+AGG 0.467542 4_1:+49875808 None:intergenic
CCACAAGGATCATACCACTA+TGG 0.467837 4_1:-49874791 Msa0553090:CDS
CAAAAGAAATGGTTTGGAGT+TGG 0.468486 4_1:+49877670 None:intergenic
TTGAATAACTGTGATGTGTT+CGG 0.472674 4_1:-49873497 Msa0553090:three_prime_UTR
ATAAGAACAGCTTGAGGGAG+TGG 0.475907 4_1:+49875503 None:intergenic
ATTTGTGTAATGCTCGTGTT+TGG 0.478781 4_1:+49873564 None:intergenic
GCAGAATGGACGGTGGTGTA+TGG 0.483733 4_1:-49874251 Msa0553090:intron
CGTGCTTATCACAGCTGATC+AGG 0.483851 4_1:-49875185 Msa0553090:CDS
GATCTGAGTGGTTGGTCCAC+CGG 0.488396 4_1:+49875051 None:intergenic
CCTCAAGCTGTTCTTATCAA+TGG 0.491577 4_1:-49875497 Msa0553090:CDS
ATCTACCAGAAGGGGTATCT+GGG 0.493408 4_1:+49874606 None:intergenic
TCCACTCGGAGTTAAACAAC+AGG 0.494721 4_1:-49877502 Msa0553090:intron
TGGTTGGTCCACCGGGAATG+GGG 0.496140 4_1:+49875059 None:intergenic
CAGACGAGTTTGCAAAGAAT+GGG 0.496723 4_1:+49873760 None:intergenic
AGCTTCAGAGGAGTGTCAGC+TGG 0.497960 4_1:+49874674 None:intergenic
GCTGATTACTTCAAGATTGG+TGG 0.498079 4_1:-49874650 Msa0553090:CDS
TTGCAGGGGATTCTTATCAA+TGG 0.500618 4_1:-49877392 Msa0553090:intron
GGGAAAGGAACAGGAATCCT+TGG 0.500790 4_1:+49875817 None:intergenic
ATGCGAGTATATTCAGATGC+TGG 0.504781 4_1:-49873946 Msa0553090:CDS
ACAGAGTAAATTTCTGGCCC+AGG 0.505625 4_1:+49877362 None:intergenic
CATGAAATTGGTTGAAGTTG+AGG 0.506111 4_1:-49875269 Msa0553090:CDS
TTATCAATGGTCAATTTCCT+GGG 0.508751 4_1:-49877379 Msa0553090:CDS
TAAATTGCAGTCCATGACTT+AGG 0.517595 4_1:+49874048 None:intergenic
AAGTAATCAGCTAGCTTCAG+AGG 0.518399 4_1:+49874662 None:intergenic
ACAAGAATGAAGTGAAATGA+AGG 0.518789 4_1:-49873606 Msa0553090:three_prime_UTR
TCATTAGCTGGTTCAGGGAA+AGG 0.520994 4_1:+49875802 None:intergenic
TGTAATCATTAGCTGGTTCA+GGG 0.522087 4_1:+49875797 None:intergenic
AGAAATGTGTAATCATTAGC+TGG 0.522917 4_1:+49875790 None:intergenic
AAGTTCTTCCCAGGAGGGAT+GGG 0.522984 4_1:+49875949 None:intergenic
GAAGTTCTTCCCAGGAGGGA+TGG 0.529815 4_1:+49875948 None:intergenic
AACAAAGCAAGTAGTTGCAT+TGG 0.537596 4_1:+49877609 None:intergenic
CCGGGAATGGGGCCTGAAAG+AGG 0.539332 4_1:+49875070 None:intergenic
CCCATCTCTTGCTTTCCACA+AGG 0.539722 4_1:-49875878 Msa0553090:CDS
GCAACAATGTAGTAGTCCTT+CGG 0.540184 4_1:+49875160 None:intergenic
GCTTTCCACAAGGCAGCTGG+TGG 0.541282 4_1:-49875868 Msa0553090:CDS
AGTTCAGCAGCACAAGTGAA+TGG 0.541477 4_1:-49874734 Msa0553090:CDS
AGGAGAAATTCTTACCAAGA+TGG 0.542021 4_1:-49875991 Msa0553090:CDS
CCCAGGAGGGATGGGACATG+TGG 0.546425 4_1:+49875957 None:intergenic
GACCAACCACTCAGATCGAT+TGG 0.547415 4_1:-49875045 Msa0553090:CDS
TATCTGGGATGCTACCAACT+TGG 0.549445 4_1:+49874621 None:intergenic
GCCTTGTGGAAAGCAAGAGA+TGG 0.551850 4_1:+49875877 None:intergenic
TGGTTCCATAAACTCCATCT+TGG 0.552233 4_1:+49875977 None:intergenic
TTATATATTAGGGACTAACT+TGG 0.558841 4_1:-49873784 Msa0553090:three_prime_UTR
AGGACAAATCTTACTGCAAG+TGG 0.560965 4_1:-49874827 Msa0553090:CDS
GTACACTGATACTTCAGTCA+TGG 0.561692 4_1:-49874579 Msa0553090:CDS
TCCAACAAGCTAGATCCATC+AGG 0.562193 4_1:-49875018 Msa0553090:intron
AGACTTAGAATATCAAATGT+TGG 0.562643 4_1:-49875331 Msa0553090:CDS
CATTGATAAGAACAGCTTGA+GGG 0.563470 4_1:+49875498 None:intergenic
GGTTGTTGGTTAACATTGTG+TGG 0.563960 4_1:+49873810 None:intergenic
TCAAAATCCTTAGTAGGCCA+AGG 0.564810 4_1:-49875834 Msa0553090:CDS
CTTCAACCAATTTCATGATG+TGG 0.567105 4_1:+49875275 None:intergenic
ATGAAAGTATCGTCCAAAGT+TGG 0.568460 4_1:-49874514 Msa0553090:CDS
TCTTCTTTGTGGCAAAGCAG+TGG 0.568771 4_1:-49873887 Msa0553090:CDS
CAAAACCACCAGCTGCCTTG+TGG 0.568925 4_1:+49875863 None:intergenic
CACAGCTGATCAGGCACCGA+AGG 0.570170 4_1:-49875176 Msa0553090:CDS
GAGTTGGACTATTACTTATG+GGG 0.570894 4_1:+49877686 None:intergenic
ATGTTGAATGAAATAAATAG+AGG 0.571312 4_1:+49877720 None:intergenic
TTAGTCCCTAATATATAACT+AGG 0.573736 4_1:+49873789 None:intergenic
TACACTGATACTTCAGTCAT+GGG 0.574862 4_1:-49874578 Msa0553090:CDS
ACCTAAGAGATGCTGTGTCT+AGG 0.579180 4_1:-49874203 Msa0553090:CDS
TATATGAATTTCAGAAGCTG+AGG 0.579611 4_1:-49875553 Msa0553090:intron
GACCAATCGATCTGAGTGGT+TGG 0.579630 4_1:+49875043 None:intergenic
CCACATGTCCCATCCCTCCT+GGG 0.583499 4_1:-49875957 Msa0553090:CDS
ACCTGTTGTTTAACTCCGAG+TGG 0.583836 4_1:+49877501 None:intergenic
ACATCATTCACTGTTGAACA+AGG 0.586268 4_1:-49875461 Msa0553090:intron
CACATACACACTTCAAGTCA+AGG 0.587068 4_1:-49875926 Msa0553090:CDS
AATGATTACACATTTCTCAT+CGG 0.587661 4_1:-49875784 Msa0553090:CDS
CCATAGTGGTATGATCCTTG+TGG 0.593975 4_1:+49874791 None:intergenic
GTGTATGTGAAGTTCTTCCC+AGG 0.595251 4_1:+49875940 None:intergenic
GATGGATCTAGCTTGTTGGA+TGG 0.598390 4_1:+49875021 None:intergenic
CCTTGTGGAAAGCAAGAGAT+GGG 0.599014 4_1:+49875878 None:intergenic
TGTACATTGATCTACCAGAA+GGG 0.599518 4_1:+49874597 None:intergenic
CACTAGACAATGTGGGAATG+TGG 0.600172 4_1:-49874020 Msa0553090:CDS
TAATGTTTATCATACCATAG+TGG 0.606188 4_1:+49874777 None:intergenic
TATGGTGACATTTATCCACT+CGG 0.612790 4_1:-49877516 Msa0553090:CDS
CCATTGATAAGAACAGCTTG+AGG 0.613338 4_1:+49875497 None:intergenic
CTTTCAGGCCCCATTCCCGG+TGG 0.617594 4_1:-49875067 Msa0553090:CDS
AAGGAAAATGTAAAGAGAGG+AGG 0.617695 4_1:+49877757 None:intergenic
ACAGGAATGGTGTTCAACAA+AGG 0.618737 4_1:-49876011 Msa0553090:intron
CATTTCTAATGCAGAATGGA+CGG 0.620989 4_1:-49874261 Msa0553090:intron
AACAAGGAAAATGTAAAGAG+AGG 0.626517 4_1:+49877754 None:intergenic
GTGTACATTGATCTACCAGA+AGG 0.628287 4_1:+49874596 None:intergenic
CTGATCAGCTGTGATAAGCA+CGG 0.630381 4_1:+49875186 None:intergenic
CTCAGGTATATCCTAAGTCA+TGG 0.631745 4_1:-49874059 Msa0553090:intron
ATGTGGGAATGTGGAATGTG+AGG 0.633524 4_1:-49874011 Msa0553090:CDS
AACTTATTATCACAATGTGA+TGG 0.634389 4_1:+49873707 None:intergenic
TGTGTCTAGGAGCACAACAC+AGG 0.641217 4_1:-49874190 Msa0553090:intron
GACTCAATAGATGTTCATGT+CGG 0.642501 4_1:-49875220 Msa0553090:CDS
ATGTGAAGTTCTTCCCAGGA+GGG 0.649193 4_1:+49875944 None:intergenic
GGACCAAGGCCAAATCCACA+AGG 0.651492 4_1:-49874806 Msa0553090:CDS
ACACGATCTCAACGAAGGCG+CGG 0.656942 4_1:+49874546 None:intergenic
ATCTTACTGCAAGTGGACCA+AGG 0.657746 4_1:-49874820 Msa0553090:CDS
TTATATGGCACTAGACAATG+TGG 0.668761 4_1:-49874028 Msa0553090:CDS
GTACATTGATCTACCAGAAG+GGG 0.678351 4_1:+49874598 None:intergenic
TTGGTACAAGATTGATCACA+CGG 0.678759 4_1:-49875758 Msa0553090:intron
CTGAAACACGATCTCAACGA+AGG 0.684228 4_1:+49874541 None:intergenic
AGAGGAAGAGAAACCCAACA+AGG 0.684746 4_1:+49877738 None:intergenic
TATGTGAAGTTCTTCCCAGG+AGG 0.689525 4_1:+49875943 None:intergenic
TATATGGCACTAGACAATGT+GGG 0.701854 4_1:-49874027 Msa0553090:CDS
TTCTAATGCAGAATGGACGG+TGG 0.708926 4_1:-49874258 Msa0553090:intron
GATGGACCAATCGATCTGAG+TGG 0.768672 4_1:+49875039 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTATTTACTTTTTATTTAAT+TGG - chr4_1:49874544-49874563 Msa0553090:CDS 10.0%
!!! AGCTAAAAAATATTTTAAAA+TGG - chr4_1:49874672-49874691 Msa0553090:CDS 10.0%
!!! GCTAAAAAATATTTTAAAAT+GGG - chr4_1:49874673-49874692 Msa0553090:CDS 10.0%
!!! AAAAGTAATTTTATTTTTCT+CGG + chr4_1:49875565-49875584 None:intergenic 10.0%
!! AATTAATTAAATTTCAATCA+TGG - chr4_1:49876972-49876991 Msa0553090:intron 10.0%
!! ATTAAAAAATTTCAATAACT+TGG + chr4_1:49877169-49877188 None:intergenic 10.0%
!!! AAGTTTTTATGAATTTCTAA+AGG + chr4_1:49874137-49874156 None:intergenic 15.0%
!!! AAATACTTGACTTTTATTTT+TGG + chr4_1:49874530-49874549 None:intergenic 15.0%
!! CATAATTAATTCTATTCATA+CGG + chr4_1:49874761-49874780 None:intergenic 15.0%
!! TGTAAAATAATTTAGAAGAA+AGG + chr4_1:49874859-49874878 None:intergenic 15.0%
!! ATTAAGTTTATGTTCAATTA+AGG + chr4_1:49875158-49875177 None:intergenic 15.0%
!! TGTAGAAAATAAATGTTAAT+TGG + chr4_1:49875893-49875912 None:intergenic 15.0%
!!! AATTAACATTTATTTTCTAC+AGG - chr4_1:49875892-49875911 Msa0553090:CDS 15.0%
!!! ATTAACATTTATTTTCTACA+GGG - chr4_1:49875893-49875912 Msa0553090:CDS 15.0%
!! GACAAAAAAATAAAACAATA+GGG + chr4_1:49877584-49877603 None:intergenic 15.0%
!! TGACAAAAAAATAAAACAAT+AGG + chr4_1:49877585-49877604 None:intergenic 15.0%
!! ATGTTGAATGAAATAAATAG+AGG + chr4_1:49873539-49873558 None:intergenic 20.0%
!!! TTTTATAATTTTGCATTTGC+AGG - chr4_1:49873848-49873867 Msa0553090:exon 20.0%
!!! TTTATAATTTTGCATTTGCA+GGG - chr4_1:49873849-49873868 Msa0553090:exon 20.0%
!! AAATTTGATTCCTTATACAA+TGG + chr4_1:49874112-49874131 None:intergenic 20.0%
!! AGAAATTCATAAAAACTTGA+TGG - chr4_1:49874139-49874158 Msa0553090:intron 20.0%
!! GAAATTCATAAAAACTTGAT+GGG - chr4_1:49874140-49874159 Msa0553090:intron 20.0%
!!! GATTAAACTTTTTTGTTTGT+CGG - chr4_1:49874801-49874820 Msa0553090:CDS 20.0%
!!! ATTAAACTTTTTTGTTTGTC+GGG - chr4_1:49874802-49874821 Msa0553090:CDS 20.0%
!! TGAATTGAATAAAAGGTTTA+AGG + chr4_1:49874940-49874959 None:intergenic 20.0%
!!! TTTTCATATTGAAATGAAAC+AGG - chr4_1:49875227-49875246 Msa0553090:CDS 20.0%
!!! TTTCTTAGTAACTGAATTTT+AGG - chr4_1:49875861-49875880 Msa0553090:CDS 20.0%
!!! ATTTCCATCAATTTTTTATC+CGG - chr4_1:49876822-49876841 Msa0553090:intron 20.0%
!! TAATGATATTCAAAGATCAT+AGG + chr4_1:49877128-49877147 None:intergenic 20.0%
!! AACAACCTAGTTATATATTA+GGG - chr4_1:49877462-49877481 Msa0553090:intron 20.0%
!! AAAAAAATAAAACAATAGGG+TGG + chr4_1:49877581-49877600 None:intergenic 20.0%
!! GTTATTCAAATCCAAATAAT+TGG + chr4_1:49877748-49877767 None:intergenic 20.0%
! AGAAAAAACAGCAAAAGAAA+TGG + chr4_1:49873600-49873619 None:intergenic 25.0%
!! TTTTTCTCTGTTTAACATGA+AGG - chr4_1:49873613-49873632 Msa0553090:three_prime_UTR 25.0%
!!! AAAAGATTAAGCATTGATCA+CGG + chr4_1:49873831-49873850 None:intergenic 25.0%
!! TTATAATTTTGCATTTGCAG+GGG - chr4_1:49873850-49873869 Msa0553090:exon 25.0%
! TTAGTAACAGAGTAAATTTC+TGG + chr4_1:49873903-49873922 None:intergenic 25.0%
! TGTTACCATGATAAAAGAAA+TGG + chr4_1:49873966-49873985 None:intergenic 25.0%
! ACAACAAGTATATCAAGTTT+GGG + chr4_1:49874360-49874379 None:intergenic 25.0%
! AACAACAAGTATATCAAGTT+TGG + chr4_1:49874361-49874380 None:intergenic 25.0%
!! GGAATAATCACACAAAATTT+TGG - chr4_1:49874459-49874478 Msa0553090:intron 25.0%
! CTCATTGTAATTTCATATGT+TGG + chr4_1:49874606-49874625 None:intergenic 25.0%
!!! AATTATTTTACAACGACGTT+TGG - chr4_1:49874867-49874886 Msa0553090:intron 25.0%
! TAGTGAGTGAATTGAATAAA+AGG + chr4_1:49874947-49874966 None:intergenic 25.0%
!! TTTTGTTAGCAAAAAATGGT+TGG + chr4_1:49874976-49874995 None:intergenic 25.0%
!! TGGTTTTTGTTAGCAAAAAA+TGG + chr4_1:49874980-49874999 None:intergenic 25.0%
!!! TTTTTTGCTAACAAAAACCA+TGG - chr4_1:49874980-49874999 Msa0553090:intron 25.0%
!! TTTTTGCTAACAAAAACCAT+GGG - chr4_1:49874981-49875000 Msa0553090:intron 25.0%
! AGAGAGAATAATGCATAAAA+AGG + chr4_1:49875098-49875117 None:intergenic 25.0%
! ATATTGAAATGAAACAGGAA+TGG - chr4_1:49875232-49875251 Msa0553090:CDS 25.0%
! AATGATTACACATTTCTCAT+CGG - chr4_1:49875472-49875491 Msa0553090:CDS 25.0%
! AGACTTAGAATATCAAATGT+TGG - chr4_1:49875925-49875944 Msa0553090:CDS 25.0%
! TAATGTTTATCATACCATAG+TGG + chr4_1:49876482-49876501 None:intergenic 25.0%
! AAATCCGGATAAAAAATTGA+TGG + chr4_1:49876829-49876848 None:intergenic 25.0%
! TTCAAACTTCATTCTCATAA+TGG + chr4_1:49877409-49877428 None:intergenic 25.0%
! CTAATATATAACTAGGTTGT+TGG + chr4_1:49877463-49877482 None:intergenic 25.0%
! CAACAACCTAGTTATATATT+AGG - chr4_1:49877461-49877480 Msa0553090:intron 25.0%
! TTAGTCCCTAATATATAACT+AGG + chr4_1:49877470-49877489 None:intergenic 25.0%
! TTATATATTAGGGACTAACT+TGG - chr4_1:49877472-49877491 Msa0553090:intron 25.0%
! AACTTATTATCACAATGTGA+TGG + chr4_1:49877552-49877571 None:intergenic 25.0%
! AACTGAAGTTTCCAATTATT+TGG - chr4_1:49877734-49877753 Msa0553090:five_prime_UTR 25.0%
AACAAGGAAAATGTAAAGAG+AGG + chr4_1:49873505-49873524 None:intergenic 30.0%
! TCTCTTTACATTTTCCTTGT+TGG - chr4_1:49873504-49873523 Msa0553090:three_prime_UTR 30.0%
! CTCTTTACATTTTCCTTGTT+GGG - chr4_1:49873505-49873524 Msa0553090:three_prime_UTR 30.0%
AAACAGCAAAAGAAATGGTT+TGG + chr4_1:49873595-49873614 None:intergenic 30.0%
ATCCTTATAGATTCTTTGAC+TGG - chr4_1:49873708-49873727 Msa0553090:three_prime_UTR 30.0%
TTTGACTGGACTATTACTTA+TGG - chr4_1:49873722-49873741 Msa0553090:three_prime_UTR 30.0%
TTATCAATGGTCAATTTCCT+GGG - chr4_1:49873877-49873896 Msa0553090:CDS 30.0%
CATGATAAAAGAAATGGTTC+TGG + chr4_1:49873960-49873979 None:intergenic 30.0%
! CAGAACCATTTCTTTTATCA+TGG - chr4_1:49873958-49873977 Msa0553090:CDS 30.0%
ATAAAAGACACCTTCACAAA+AGG + chr4_1:49874181-49874200 None:intergenic 30.0%
!!! ACACCAACTACTTTTTATGA+AGG - chr4_1:49874230-49874249 Msa0553090:CDS 30.0%
ACACCTTCATAAAAAGTAGT+TGG + chr4_1:49874236-49874255 None:intergenic 30.0%
!!! CACGGTACTATTTTTTTCTT+TGG - chr4_1:49874438-49874457 Msa0553090:intron 30.0%
ACAGTTGAGTTATTACTAGA+GGG - chr4_1:49874723-49874742 Msa0553090:CDS 30.0%
! TTTAAGGTGACAGCAAAAAA+GGG + chr4_1:49874924-49874943 None:intergenic 30.0%
AAAAAGGCTTTCACTTAGTT+GGG + chr4_1:49875082-49875101 None:intergenic 30.0%
TAAAAAGGCTTTCACTTAGT+TGG + chr4_1:49875083-49875102 None:intergenic 30.0%
AGAAATGTGTAATCATTAGC+TGG + chr4_1:49875469-49875488 None:intergenic 30.0%
AGTCACGAGAGAAATAAATT+GGG + chr4_1:49875633-49875652 None:intergenic 30.0%
AAGTCACGAGAGAAATAAAT+TGG + chr4_1:49875634-49875653 None:intergenic 30.0%
TATATGAATTTCAGAAGCTG+AGG - chr4_1:49875703-49875722 Msa0553090:intron 30.0%
TACAACATACACTCAACTTT+AGG - chr4_1:49875950-49875969 Msa0553090:CDS 30.0%
ATGAAATTGGTTGAAGTTGA+GGG - chr4_1:49875988-49876007 Msa0553090:CDS 30.0%
ATGAACATCTATTGAGTCAT+AGG + chr4_1:49876034-49876053 None:intergenic 30.0%
TTCAACTCGTTTCACTAATA+GGG - chr4_1:49876119-49876138 Msa0553090:intron 30.0%
AATTGAACTCAATTACCTGA+TGG + chr4_1:49876256-49876275 None:intergenic 30.0%
! GTTCAATTTTTATACACCGT+CGG - chr4_1:49876268-49876287 Msa0553090:intron 30.0%
ATGAAAAGTCTCTTAGAGAT+TGG + chr4_1:49876304-49876323 None:intergenic 30.0%
! ATTAAGTTGTTGCAATTCAC+AGG - chr4_1:49876409-49876428 Msa0553090:intron 30.0%
!! ACATTGATGGATACTCATTT+TGG - chr4_1:49876766-49876785 Msa0553090:intron 30.0%
!! CATTGATGGATACTCATTTT+GGG - chr4_1:49876767-49876786 Msa0553090:intron 30.0%
TGTAGCATAGTGATTAAATC+CGG + chr4_1:49876844-49876863 None:intergenic 30.0%
!! TTGTATTGGTTACGACTATT+AGG + chr4_1:49877034-49877053 None:intergenic 30.0%
TCATTATACTAGCTAAATGC+AGG - chr4_1:49877142-49877161 Msa0553090:intron 30.0%
!!! TTTTTTAATTTGTGTGCCTC+AGG - chr4_1:49877180-49877199 Msa0553090:intron 30.0%
ACAAGAATGAAGTGAAATGA+AGG - chr4_1:49877650-49877669 Msa0553090:five_prime_UTR 30.0%
TTGAATAACTGTGATGTGTT+CGG - chr4_1:49877759-49877778 Msa0553090:five_prime_UTR 30.0%
AAGGAAAATGTAAAGAGAGG+AGG + chr4_1:49873502-49873521 None:intergenic 35.0%
GAGTTGGACTATTACTTATG+GGG + chr4_1:49873573-49873592 None:intergenic 35.0%
GGAGTTGGACTATTACTTAT+GGG + chr4_1:49873574-49873593 None:intergenic 35.0%
TGGAGTTGGACTATTACTTA+TGG + chr4_1:49873575-49873594 None:intergenic 35.0%
! CAAAAGAAATGGTTTGGAGT+TGG + chr4_1:49873589-49873608 None:intergenic 35.0%
AACAAAGCAAGTAGTTGCAT+TGG + chr4_1:49873650-49873669 None:intergenic 35.0%
GTCCAGTCAAAGAATCTATA+AGG + chr4_1:49873713-49873732 None:intergenic 35.0%
TATGGTGACATTTATCCACT+CGG - chr4_1:49873740-49873759 Msa0553090:three_prime_UTR 35.0%
CTTATCAATGGTCAATTTCC+TGG - chr4_1:49873876-49873895 Msa0553090:CDS 35.0%
GATAAGTGAGCCATTGTATA+AGG - chr4_1:49874099-49874118 Msa0553090:intron 35.0%
! ATAGAAGACACCTTTTGTGA+AGG - chr4_1:49874168-49874187 Msa0553090:intron 35.0%
AAAGTGAAAATCACACCTTG+AGG - chr4_1:49874279-49874298 Msa0553090:intron 35.0%
! TTTTATCAAGTCACACCTCA+AGG + chr4_1:49874297-49874316 None:intergenic 35.0%
!!! CACCACACTACTTTTTATGA+AGG - chr4_1:49874319-49874338 Msa0553090:intron 35.0%
CACCTTCATAAAAAGTAGTG+TGG + chr4_1:49874324-49874343 None:intergenic 35.0%
GATATACCTGAATACACACA+CGG - chr4_1:49874420-49874439 Msa0553090:intron 35.0%
!! GTTTTAATGAGCAAAGAACG+AGG - chr4_1:49874566-49874585 Msa0553090:CDS 35.0%
CACAGTTGAGTTATTACTAG+AGG - chr4_1:49874722-49874741 Msa0553090:CDS 35.0%
TTACAACGACGTTTGGATTA+AGG - chr4_1:49874874-49874893 Msa0553090:intron 35.0%
TACAACGACGTTTGGATTAA+GGG - chr4_1:49874875-49874894 Msa0553090:intron 35.0%
ACAGCAAAAAAGGGAATATG+AGG + chr4_1:49874915-49874934 None:intergenic 35.0%
! GTTTAAGGTGACAGCAAAAA+AGG + chr4_1:49874925-49874944 None:intergenic 35.0%
AGTAAAAATCATCACGTGTG+AGG - chr4_1:49875126-49875145 Msa0553090:CDS 35.0%
AGGAGAAATTCTTACCAAGA+TGG - chr4_1:49875265-49875284 Msa0553090:CDS 35.0%
TCTTACCAAGATGGAGTTTA+TGG - chr4_1:49875274-49875293 Msa0553090:CDS 35.0%
CTTCAAGTCAAGGATCAAAT+TGG - chr4_1:49875340-49875359 Msa0553090:CDS 35.0%
GATCCATCAAAATCCTTAGT+AGG - chr4_1:49875416-49875435 Msa0553090:intron 35.0%
! TGTAATCATTAGCTGGTTCA+GGG + chr4_1:49875462-49875481 None:intergenic 35.0%
TTGGTACAAGATTGATCACA+CGG - chr4_1:49875498-49875517 Msa0553090:CDS 35.0%
!!! CTGAGGAAAGTTCTAGATTT+TGG - chr4_1:49875720-49875739 Msa0553090:intron 35.0%
CATTGATAAGAACAGCTTGA+GGG + chr4_1:49875761-49875780 None:intergenic 35.0%
ACATCATTCACTGTTGAACA+AGG - chr4_1:49875795-49875814 Msa0553090:CDS 35.0%
!! ACACTCAACTTTAGGATTCA+AGG - chr4_1:49875958-49875977 Msa0553090:CDS 35.0%
CTTCAACCAATTTCATGATG+TGG + chr4_1:49875984-49876003 None:intergenic 35.0%
CATGAAATTGGTTGAAGTTG+AGG - chr4_1:49875987-49876006 Msa0553090:CDS 35.0%
GACTCAATAGATGTTCATGT+CGG - chr4_1:49876036-49876055 Msa0553090:intron 35.0%
CTTCAACTCGTTTCACTAAT+AGG - chr4_1:49876118-49876137 Msa0553090:intron 35.0%
GATTGGATTATTTAGACCGA+CGG + chr4_1:49876287-49876306 None:intergenic 35.0%
CTAGCTGATTACTTCAAGAT+TGG - chr4_1:49876603-49876622 Msa0553090:intron 35.0%
TGTACATTGATCTACCAGAA+GGG + chr4_1:49876662-49876681 None:intergenic 35.0%
TACACTGATACTTCAGTCAT+GGG - chr4_1:49876678-49876697 Msa0553090:intron 35.0%
AACTTTGGACGATACTTTCA+TGG + chr4_1:49876743-49876762 None:intergenic 35.0%
!! ATGAAAGTATCGTCCAAAGT+TGG - chr4_1:49876742-49876761 Msa0553090:intron 35.0%
ATGGCATTTCTAATGCAGAA+TGG - chr4_1:49876991-49877010 Msa0553090:intron 35.0%
CATTTCTAATGCAGAATGGA+CGG - chr4_1:49876995-49877014 Msa0553090:intron 35.0%
TAAATTGCAGTCCATGACTT+AGG + chr4_1:49877211-49877230 None:intergenic 35.0%
! GTCATGGACTGCAATTTATA+TGG - chr4_1:49877213-49877232 Msa0553090:intron 35.0%
TTATATGGCACTAGACAATG+TGG - chr4_1:49877228-49877247 Msa0553090:intron 35.0%
TATATGGCACTAGACAATGT+GGG - chr4_1:49877229-49877248 Msa0553090:intron 35.0%
! GAATGTGAGGAGTGAATTTT+GGG - chr4_1:49877258-49877277 Msa0553090:intron 35.0%
ATTCAGATGCTGGATCAATT+AGG - chr4_1:49877320-49877339 Msa0553090:CDS 35.0%
TTCAGATGCTGGATCAATTA+GGG - chr4_1:49877321-49877340 Msa0553090:CDS 35.0%
!! AGAAGAGCATTCTTTGGAAT+TGG + chr4_1:49877355-49877374 None:intergenic 35.0%
ATTTGTGTAATGCTCGTGTT+TGG + chr4_1:49877695-49877714 None:intergenic 35.0%
TTGCAGGGGATTCTTATCAA+TGG - chr4_1:49873864-49873883 Msa0553090:CDS 40.0%
ATAGTACCGTGTGTGTATTC+AGG + chr4_1:49874429-49874448 None:intergenic 40.0%
GGGTGCATTTATTAAACCCA+TGG + chr4_1:49875000-49875019 None:intergenic 40.0%
GATCCGTGACATGACATTAA+GGG + chr4_1:49875040-49875059 None:intergenic 40.0%
TGATCCGTGACATGACATTA+AGG + chr4_1:49875041-49875060 None:intergenic 40.0%
ACAGGAATGGTGTTCAACAA+AGG - chr4_1:49875245-49875264 Msa0553090:CDS 40.0%
TGGTTCCATAAACTCCATCT+TGG + chr4_1:49875282-49875301 None:intergenic 40.0%
CACATACACACTTCAAGTCA+AGG - chr4_1:49875330-49875349 Msa0553090:CDS 40.0%
! TGGCCTACTAAGGATTTTGA+TGG + chr4_1:49875422-49875441 None:intergenic 40.0%
TCAAAATCCTTAGTAGGCCA+AGG - chr4_1:49875422-49875441 Msa0553090:intron 40.0%
! GTGTAATCATTAGCTGGTTC+AGG + chr4_1:49875463-49875482 None:intergenic 40.0%
ACACATTTCTCATCGGTGAT+TGG - chr4_1:49875479-49875498 Msa0553090:CDS 40.0%
CCATTGATAAGAACAGCTTG+AGG + chr4_1:49875762-49875781 None:intergenic 40.0%
CCTCAAGCTGTTCTTATCAA+TGG - chr4_1:49875759-49875778 Msa0553090:CDS 40.0%
CTTATCAATGGTCGTGCAAA+TGG - chr4_1:49875771-49875790 Msa0553090:CDS 40.0%
TCAAGGCCACATCATGAAAT+TGG - chr4_1:49875975-49875994 Msa0553090:CDS 40.0%
GCAACAATGTAGTAGTCCTT+CGG + chr4_1:49876099-49876118 None:intergenic 40.0%
AACTCACAACAACCTCTTTC+AGG - chr4_1:49876174-49876193 Msa0553090:intron 40.0%
AGGACAAATCTTACTGCAAG+TGG - chr4_1:49876429-49876448 Msa0553090:intron 40.0%
TGGTATGATCCTTGTGGATT+TGG + chr4_1:49876462-49876481 None:intergenic 40.0%
! AAGTAATCAGCTAGCTTCAG+AGG + chr4_1:49876597-49876616 None:intergenic 40.0%
GCTGATTACTTCAAGATTGG+TGG - chr4_1:49876606-49876625 Msa0553090:intron 40.0%
!!! ATTGGTGGTGTTTTCCAAGT+TGG - chr4_1:49876621-49876640 Msa0553090:intron 40.0%
GTACATTGATCTACCAGAAG+GGG + chr4_1:49876661-49876680 None:intergenic 40.0%
GTGTACATTGATCTACCAGA+AGG + chr4_1:49876663-49876682 None:intergenic 40.0%
GTACACTGATACTTCAGTCA+TGG - chr4_1:49876677-49876696 Msa0553090:intron 40.0%
ATCCATCAATGTGCCAACTT+TGG + chr4_1:49876758-49876777 None:intergenic 40.0%
!!! GGATACTCATTTTGGGTTGT+CGG - chr4_1:49876774-49876793 Msa0553090:intron 40.0%
!!! GATACTCATTTTGGGTTGTC+GGG - chr4_1:49876775-49876794 Msa0553090:intron 40.0%
! CAGCATCTCTTAGGTTGTAT+TGG + chr4_1:49877048-49877067 None:intergenic 40.0%
CATGACTTAGGATATACCTG+AGG + chr4_1:49877199-49877218 None:intergenic 40.0%
CTCAGGTATATCCTAAGTCA+TGG - chr4_1:49877197-49877216 Msa0553090:intron 40.0%
! GGAATGTGAGGAGTGAATTT+TGG - chr4_1:49877257-49877276 Msa0553090:intron 40.0%
! TTTTGGGCACGACAATATCT+TGG - chr4_1:49877274-49877293 Msa0553090:intron 40.0%
ATGCGAGTATATTCAGATGC+TGG - chr4_1:49877310-49877329 Msa0553090:CDS 40.0%
!! CCACAAAGAAGAGCATTCTT+TGG + chr4_1:49877361-49877380 None:intergenic 40.0%
CCAAAGAATGCTCTTCTTTG+TGG - chr4_1:49877358-49877377 Msa0553090:CDS 40.0%
TGGTTAACATTGTGTGGTGT+AGG + chr4_1:49877443-49877462 None:intergenic 40.0%
! GGTTGTTGGTTAACATTGTG+TGG + chr4_1:49877449-49877468 None:intergenic 40.0%
CAGACGAGTTTGCAAAGAAT+GGG + chr4_1:49877499-49877518 None:intergenic 40.0%
ACAGACGAGTTTGCAAAGAA+TGG + chr4_1:49877500-49877519 None:intergenic 40.0%
AGAGGAAGAGAAACCCAACA+AGG + chr4_1:49873521-49873540 None:intergenic 45.0%
TCCACTCGGAGTTAAACAAC+AGG - chr4_1:49873754-49873773 Msa0553090:three_prime_UTR 45.0%
! ACCTGTTGTTTAACTCCGAG+TGG + chr4_1:49873758-49873777 None:intergenic 45.0%
!! ACAGAGTAAATTTCTGGCCC+AGG + chr4_1:49873897-49873916 None:intergenic 45.0%
GACATTAAGGGCCTAAGTGA+TGG + chr4_1:49875028-49875047 None:intergenic 45.0%
AGGCCCTTAATGTCATGTCA+CGG - chr4_1:49875034-49875053 Msa0553090:CDS 45.0%
ATGTGAAGTTCTTCCCAGGA+GGG + chr4_1:49875315-49875334 None:intergenic 45.0%
TATGTGAAGTTCTTCCCAGG+AGG + chr4_1:49875316-49875335 None:intergenic 45.0%
GTGTATGTGAAGTTCTTCCC+AGG + chr4_1:49875319-49875338 None:intergenic 45.0%
CCTTGTGGAAAGCAAGAGAT+GGG + chr4_1:49875381-49875400 None:intergenic 45.0%
! TCATTAGCTGGTTCAGGGAA+AGG + chr4_1:49875457-49875476 None:intergenic 45.0%
GTACGTGACAATGTTCAGCT+AGG + chr4_1:49875611-49875630 None:intergenic 45.0%
ATAAGAACAGCTTGAGGGAG+TGG + chr4_1:49875756-49875775 None:intergenic 45.0%
CTGATCAGCTGTGATAAGCA+CGG + chr4_1:49876073-49876092 None:intergenic 45.0%
!! GATGGATCTAGCTTGTTGGA+TGG + chr4_1:49876238-49876257 None:intergenic 45.0%
TCCAACAAGCTAGATCCATC+AGG - chr4_1:49876238-49876257 Msa0553090:intron 45.0%
! ACCTGATGGATCTAGCTTGT+TGG + chr4_1:49876242-49876261 None:intergenic 45.0%
ATCTTACTGCAAGTGGACCA+AGG - chr4_1:49876436-49876455 Msa0553090:intron 45.0%
CCATAGTGGTATGATCCTTG+TGG + chr4_1:49876468-49876487 None:intergenic 45.0%
CCACAAGGATCATACCACTA+TGG - chr4_1:49876465-49876484 Msa0553090:intron 45.0%
AGTTCAGCAGCACAAGTGAA+TGG - chr4_1:49876522-49876541 Msa0553090:intron 45.0%
! TATCTGGGATGCTACCAACT+TGG + chr4_1:49876638-49876657 None:intergenic 45.0%
!! ATCTACCAGAAGGGGTATCT+GGG + chr4_1:49876653-49876672 None:intergenic 45.0%
CTGAAACACGATCTCAACGA+AGG + chr4_1:49876718-49876737 None:intergenic 45.0%
GTCCAAAGTTGGCACATTGA+TGG - chr4_1:49876753-49876772 Msa0553090:intron 45.0%
TTCTAATGCAGAATGGACGG+TGG - chr4_1:49876998-49877017 Msa0553090:intron 45.0%
ACCTAAGAGATGCTGTGTCT+AGG - chr4_1:49877053-49877072 Msa0553090:intron 45.0%
TCCTAGACACAGCATCTCTT+AGG + chr4_1:49877057-49877076 None:intergenic 45.0%
CACTAGACAATGTGGGAATG+TGG - chr4_1:49877236-49877255 Msa0553090:intron 45.0%
ATGTGGGAATGTGGAATGTG+AGG - chr4_1:49877245-49877264 Msa0553090:intron 45.0%
TCTTCTTTGTGGCAAAGCAG+TGG - chr4_1:49877369-49877388 Msa0553090:CDS 45.0%
CTTCTTTGTGGCAAAGCAGT+GGG - chr4_1:49877370-49877389 Msa0553090:CDS 45.0%
!! ATATTAGAATAAAAATAAAT+CGG + chr4_1:49875665-49875684 None:intergenic 5.0%
AGGGCCTAAGTGATGGTTGA+GGG + chr4_1:49875021-49875040 None:intergenic 50.0%
AAGGGCCTAAGTGATGGTTG+AGG + chr4_1:49875022-49875041 None:intergenic 50.0%
! AGTTGGGCTTGGAGTTGGTT+GGG + chr4_1:49875066-49875085 None:intergenic 50.0%
! TAGTTGGGCTTGGAGTTGGT+TGG + chr4_1:49875067-49875086 None:intergenic 50.0%
CACTTAGTTGGGCTTGGAGT+TGG + chr4_1:49875071-49875090 None:intergenic 50.0%
GGCTTTCACTTAGTTGGGCT+TGG + chr4_1:49875077-49875096 None:intergenic 50.0%
AAGTTCTTCCCAGGAGGGAT+GGG + chr4_1:49875310-49875329 None:intergenic 50.0%
CCCATCTCTTGCTTTCCACA+AGG - chr4_1:49875378-49875397 Msa0553090:intron 50.0%
GCCTTGTGGAAAGCAAGAGA+TGG + chr4_1:49875382-49875401 None:intergenic 50.0%
CAGGAATCCTTGGCCTACTA+AGG + chr4_1:49875432-49875451 None:intergenic 50.0%
GGGAAAGGAACAGGAATCCT+TGG + chr4_1:49875442-49875461 None:intergenic 50.0%
CGTGCTTATCACAGCTGATC+AGG - chr4_1:49876071-49876090 Msa0553090:intron 50.0%
GACCAACCACTCAGATCGAT+TGG - chr4_1:49876211-49876230 Msa0553090:intron 50.0%
GACCAATCGATCTGAGTGGT+TGG + chr4_1:49876216-49876235 None:intergenic 50.0%
GATGGACCAATCGATCTGAG+TGG + chr4_1:49876220-49876239 None:intergenic 50.0%
GATCCTTGTGGATTTGGCCT+TGG + chr4_1:49876456-49876475 None:intergenic 50.0%
!! GATCTACCAGAAGGGGTATC+TGG + chr4_1:49876654-49876673 None:intergenic 50.0%
! TGTGTCTAGGAGCACAACAC+AGG - chr4_1:49877066-49877085 Msa0553090:intron 50.0%
GCACCCCTCAACCATCACTT+AGG - chr4_1:49875014-49875033 Msa0553090:intron 55.0%
GGGCCTAAGTGATGGTTGAG+GGG + chr4_1:49875020-49875039 None:intergenic 55.0%
GAAGTTCTTCCCAGGAGGGA+TGG + chr4_1:49875311-49875330 None:intergenic 55.0%
CTTGCTTTCCACAAGGCAGC+TGG - chr4_1:49875385-49875404 Msa0553090:intron 55.0%
CAAAACCACCAGCTGCCTTG+TGG + chr4_1:49875396-49875415 None:intergenic 55.0%
! CACAAGGCAGCTGGTGGTTT+TGG - chr4_1:49875394-49875413 Msa0553090:intron 55.0%
! GCTGGTTCAGGGAAAGGAAC+AGG + chr4_1:49875451-49875470 None:intergenic 55.0%
! ATCTGAGTGGTTGGTCCACC+GGG + chr4_1:49876207-49876226 None:intergenic 55.0%
! GATCTGAGTGGTTGGTCCAC+CGG + chr4_1:49876208-49876227 None:intergenic 55.0%
GGACCAAGGCCAAATCCACA+AGG - chr4_1:49876450-49876469 Msa0553090:intron 55.0%
!! AGCTTCAGAGGAGTGTCAGC+TGG + chr4_1:49876585-49876604 None:intergenic 55.0%
AGCATCCCAGATACCCCTTC+TGG - chr4_1:49876645-49876664 Msa0553090:intron 55.0%
ACACGATCTCAACGAAGGCG+CGG + chr4_1:49876713-49876732 None:intergenic 55.0%
GCAGAATGGACGGTGGTGTA+TGG - chr4_1:49877005-49877024 Msa0553090:intron 55.0%
ACCACATGTCCCATCCCTCC+TGG - chr4_1:49875298-49875317 Msa0553090:CDS 60.0%
CCACATGTCCCATCCCTCCT+GGG - chr4_1:49875299-49875318 Msa0553090:CDS 60.0%
GCTTTCCACAAGGCAGCTGG+TGG - chr4_1:49875388-49875407 Msa0553090:intron 60.0%
!! CACAGCTGATCAGGCACCGA+AGG - chr4_1:49876080-49876099 Msa0553090:intron 60.0%
CCTCTTTCAGGCCCCATTCC+CGG - chr4_1:49876186-49876205 Msa0553090:intron 60.0%
! TGGTTGGTCCACCGGGAATG+GGG + chr4_1:49876200-49876219 None:intergenic 60.0%
! GTGGTTGGTCCACCGGGAAT+GGG + chr4_1:49876201-49876220 None:intergenic 60.0%
! AGTGGTTGGTCCACCGGGAA+TGG + chr4_1:49876202-49876221 None:intergenic 60.0%
CCCAGGAGGGATGGGACATG+TGG + chr4_1:49875302-49875321 None:intergenic 65.0%
CCGGGAATGGGGCCTGAAAG+AGG + chr4_1:49876189-49876208 None:intergenic 65.0%
CTTTCAGGCCCCATTCCCGG+TGG - chr4_1:49876189-49876208 Msa0553090:intron 65.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 49873485 49877793 49873485 ID=Msa0553090;Name=Msa0553090
chr4_1 mRNA 49873485 49877793 49873485 ID=Msa0553090-mRNA-1;Parent=Msa0553090;Name=Msa0553090-mRNA-1;_AED=0.10;_eAED=0.10;_QI=144|1|1|1|1|1|8|379|543
chr4_1 exon 49873485 49874076 49873485 ID=Msa0553090-mRNA-1:exon:14088;Parent=Msa0553090-mRNA-1
chr4_1 exon 49874191 49874269 49874191 ID=Msa0553090-mRNA-1:exon:14087;Parent=Msa0553090-mRNA-1
chr4_1 exon 49874482 49874847 49874482 ID=Msa0553090-mRNA-1:exon:14086;Parent=Msa0553090-mRNA-1
chr4_1 exon 49875019 49875364 49875019 ID=Msa0553090-mRNA-1:exon:14085;Parent=Msa0553090-mRNA-1
chr4_1 exon 49875462 49875561 49875462 ID=Msa0553090-mRNA-1:exon:14084;Parent=Msa0553090-mRNA-1
chr4_1 exon 49875759 49876029 49875759 ID=Msa0553090-mRNA-1:exon:14083;Parent=Msa0553090-mRNA-1
chr4_1 exon 49877299 49877408 49877299 ID=Msa0553090-mRNA-1:exon:14082;Parent=Msa0553090-mRNA-1
chr4_1 exon 49877503 49877793 49877503 ID=Msa0553090-mRNA-1:exon:14081;Parent=Msa0553090-mRNA-1
chr4_1 five_prime_UTR 49877650 49877793 49877650 ID=Msa0553090-mRNA-1:five_prime_utr;Parent=Msa0553090-mRNA-1
chr4_1 CDS 49877503 49877649 49877503 ID=Msa0553090-mRNA-1:cds;Parent=Msa0553090-mRNA-1
chr4_1 CDS 49877299 49877408 49877299 ID=Msa0553090-mRNA-1:cds;Parent=Msa0553090-mRNA-1
chr4_1 CDS 49875759 49876029 49875759 ID=Msa0553090-mRNA-1:cds;Parent=Msa0553090-mRNA-1
chr4_1 CDS 49875462 49875561 49875462 ID=Msa0553090-mRNA-1:cds;Parent=Msa0553090-mRNA-1
chr4_1 CDS 49875019 49875364 49875019 ID=Msa0553090-mRNA-1:cds;Parent=Msa0553090-mRNA-1
chr4_1 CDS 49874482 49874847 49874482 ID=Msa0553090-mRNA-1:cds;Parent=Msa0553090-mRNA-1
chr4_1 CDS 49874191 49874269 49874191 ID=Msa0553090-mRNA-1:cds;Parent=Msa0553090-mRNA-1
chr4_1 CDS 49873864 49874076 49873864 ID=Msa0553090-mRNA-1:cds;Parent=Msa0553090-mRNA-1
chr4_1 three_prime_UTR 49873485 49873863 49873485 ID=Msa0553090-mRNA-1:three_prime_utr;Parent=Msa0553090-mRNA-1
Gene Sequence

>Msa0553090

ATGAAGGCGATGAATGAACCAATGCAACTACTTGCTTTGTTGAGTTTCTGCTCAATTTTTTTTCTTGTTGCTGCTGAAGATCCTTATAGATTCTTTGACTGGACTATTACTTATGGTGACATTTATCCACTCGGAGTTAAACAACAGGGGATTCTTATCAATGGTCAATTTCCTGGGCCAGAAATTTACTCTGTTACTAATGACAATTTGATCATCAATGTGCACAATAATTTGCCAGAACCATTTCTTTTATCATGGAATGGTGTTCAACAAAGGAGAAATTCTTACCAAGATGGAGTTTATGGAACCACATGTCCCATCCCTCCTGGGAAGAACTTCACATACACACTTCAAGTCAAGGATCAAATTGGAAGCTTCTTTTATTTCCCATCTCTTGCTTTCCACAAGGCAGCTGGTGGTTTTGGATCCATCAAAATCCTTAGTAGGCCAAGGATTCCTGTTCCTTTCCCTGAACCAGCTAATGATTACACATTTCTCATCGGTGATTGGTACAAGATTGATCACACGAAGCTGAGGAAAGTTCTAGATTTTGGACACAAACTTCCACTCCCTCAAGCTGTTCTTATCAATGGTCGTGCAAATGGCACATCATTCACTGTTGAACAAGGGAAAACATACAGACTTAGAATATCAAATGTTGGACTACAACATACACTCAACTTTAGGATTCAAGGCCACATCATGAAATTGGTTGAAGTTGAGGGAACTCACACTGTACAAACATCCTATGACTCAATAGATGTTCATGTCGGACAATCTTACTCCGTGCTTATCACAGCTGATCAGGCACCGAAGGACTACTACATTGTTGCTTCAACTCGTTTCACTAATAGGGTTCTCACAAGCACTGCAGCACTTCACTACAGTAACTCACAACAACCTCTTTCAGGCCCCATTCCCGGTGGACCAACCACTCAGATCGATTGGTCCATCCAACAAGCTAGATCCATCAGGACAAATCTTACTGCAAGTGGACCAAGGCCAAATCCACAAGGATCATACCACTATGGTATGATAAACATTAGCAGAACAATTAAGCTTGAGAGTTCAGCAGCACAAGTGAATGGCAAGCAAAGATATGCAGTCAACAGTGTATCTTTCATACCAGCTGACACTCCTCTGAAGCTAGCTGATTACTTCAAGATTGGTGGTGTTTTCCAAGTTGGTAGCATCCCAGATACCCCTTCTGGTAGATCAATGTACACTGATACTTCAGTCATGGGTGCTGATTTCCGCGCCTTCGTTGAGATCGTGTTTCAGAACCATGAAAGTATCGTCCAAAGTTGGCACATTGATGGATACTCATTTTGGGTTGTCGGAATGGACGGTGGTGTATGGACGCCTAATAGTCGTAACCAATACAACCTAAGAGATGCTGTGTCTAGGAGCACAACACAGGTATATCCTAAGTCATGGACTGCAATTTATATGGCACTAGACAATGTGGGAATGTGGAATGTGAGGAGTGAATTTTGGGCACGACAATATCTTGGACAACAATTCTATATGCGAGTATATTCAGATGCTGGATCAATTAGGGATGAATATCCAATTCCAAAGAATGCTCTTCTTTGTGGCAAAGCAGTGGGCAGAGAAACTAGACCATTATGA
Protein sequence

>Msa0553090

MKAMNEPMQLLALLSFCSIFFLVAAEDPYRFFDWTITYGDIYPLGVKQQGILINGQFPGPEIYSVTNDNLIINVHNNLPEPFLLSWNGVQQRRNSYQDGVYGTTCPIPPGKNFTYTLQVKDQIGSFFYFPSLAFHKAAGGFGSIKILSRPRIPVPFPEPANDYTFLIGDWYKIDHTKLRKVLDFGHKLPLPQAVLINGRANGTSFTVEQGKTYRLRISNVGLQHTLNFRIQGHIMKLVEVEGTHTVQTSYDSIDVHVGQSYSVLITADQAPKDYYIVASTRFTNRVLTSTAALHYSNSQQPLSGPIPGGPTTQIDWSIQQARSIRTNLTASGPRPNPQGSYHYGMINISRTIKLESSAAQVNGKQRYAVNSVSFIPADTPLKLADYFKIGGVFQVGSIPDTPSGRSMYTDTSVMGADFRAFVEIVFQNHESIVQSWHIDGYSFWVVGMDGGVWTPNSRNQYNLRDAVSRSTTQVYPKSWTAIYMALDNVGMWNVRSEFWARQYLGQQFYMRVYSDAGSIRDEYPIPKNALLCGKAVGRETRPL*