AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1453050

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1453050 MtrunA17_Chr4g0038671 98.986 296 3 0 1 296 248 543 0.0 606
Msa1453050 MtrunA17_Chr3g0143671 74.324 296 76 0 1 296 249 544 3.48e-165 469
Msa1453050 MtrunA17_Chr1g0157341 77.703 296 66 0 1 296 248 543 1.42e-161 460
Msa1453050 MtrunA17_Chr2g0322441 74.138 290 75 0 2 291 250 539 4.16e-160 456
Msa1453050 MtrunA17_Chr4g0024861 72.165 291 81 0 2 292 247 537 3.27e-159 454
Msa1453050 MtrunA17_Chr4g0024641 72.125 287 80 0 3 289 171 457 2.40e-150 445
Msa1453050 MtrunA17_Chr4g0024641 67.470 83 27 0 211 293 875 957 4.18e-31 122
Msa1453050 MtrunA17_Chr4g0024641 68.182 88 26 2 204 289 804 891 1.23e-30 121
Msa1453050 MtrunA17_Chr4g0024641 69.620 79 24 0 211 289 503 581 3.55e-30 120
Msa1453050 MtrunA17_Chr4g0024641 69.620 79 24 0 211 289 565 643 3.55e-30 120
Msa1453050 MtrunA17_Chr4g0024641 69.620 79 24 0 211 289 627 705 3.55e-30 120
Msa1453050 MtrunA17_Chr4g0024641 69.620 79 24 0 211 289 689 767 3.55e-30 120
Msa1453050 MtrunA17_Chr4g0024641 68.354 79 25 0 211 289 751 829 4.23e-29 117
Msa1453050 MtrunA17_Chr4g0024641 68.354 79 25 0 211 289 441 519 4.39e-29 117
Msa1453050 MtrunA17_Chr4g0075071 69.492 295 90 0 2 296 251 545 1.60e-148 427
Msa1453050 MtrunA17_Chr8g0374271 61.168 291 112 1 3 292 250 540 3.25e-125 368
Msa1453050 MtrunA17_Chr5g0402831 59.655 290 115 1 3 292 250 537 2.40e-120 355
Msa1453050 MtrunA17_Chr8g0374261 56.250 288 124 2 2 288 248 534 3.78e-112 334
Msa1453050 MtrunA17_Chr4g0043251 53.819 288 129 3 2 286 252 538 3.51e-106 319
Msa1453050 MtrunA17_Chr4g0043261 52.265 287 133 3 3 286 247 532 1.55e-98 300
Msa1453050 MtrunA17_Chr3g0121151 50.871 287 137 3 3 286 292 577 2.53e-97 298
Msa1453050 MtrunA17_Chr3g0121161 50.174 287 139 3 3 286 292 577 1.21e-95 293
Msa1453050 MtrunA17_Chr3g0121171 51.568 287 135 3 3 286 252 537 9.61e-92 297
Msa1453050 MtrunA17_Chr3g0121171 50.523 287 138 3 3 286 827 1112 2.62e-91 296
Msa1453050 MtrunA17_Chr3g0121171 50.523 287 138 3 3 286 1402 1687 6.56e-91 295
Msa1453050 MtrunA17_Chr3g0121171 50.523 287 138 3 3 286 1968 2253 1.75e-90 294
Msa1453050 MtrunA17_Chr3g0079321 49.488 293 139 5 2 286 261 552 1.95e-88 275
Msa1453050 MtrunA17_Chr4g0056511 46.939 294 147 5 2 287 259 551 3.50e-87 271
Msa1453050 MtrunA17_Chr1g0197301 47.203 286 148 3 4 286 45 330 1.75e-83 254
Msa1453050 MtrunA17_Chr7g0234061 47.079 291 146 6 2 286 261 549 3.26e-82 259
Msa1453050 MtrunA17_Chr3g0118321 25.987 304 189 10 4 286 275 563 2.58e-23 99.8
Msa1453050 MtrunA17_Chr3g0118331 24.585 301 197 6 4 286 274 562 2.06e-20 91.3
Msa1453050 MtrunA17_Chr3g0118351 24.390 328 190 10 2 286 22 334 1.91e-19 87.0
Msa1453050 MtrunA17_Chr5g0404341 28.387 310 184 11 5 285 266 566 8.31e-19 86.3
Msa1453050 MtrunA17_Chr5g0404361 28.247 308 186 11 5 285 312 611 1.93e-18 85.5
Msa1453050 MtrunA17_Chr8g0382691 23.625 309 192 9 8 290 282 572 5.18e-15 75.1
Msa1453050 MtrunA17_Chr3g0104061 25.083 303 203 8 4 285 257 556 1.39e-14 73.9
Msa1453050 MtrunA17_Chr5g0429511 25.246 305 201 9 4 285 258 558 2.34e-14 73.2
Msa1453050 MtrunA17_Chr5g0436461 25.566 309 195 11 4 285 254 554 2.75e-14 72.8
Msa1453050 MtrunA17_Chr4g0005921 25.083 303 206 6 4 285 255 557 3.58e-13 69.7
Msa1453050 MtrunA17_Chr5g0431581 27.974 311 175 15 11 285 263 560 9.18e-12 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1453050 AT1G76160.1 74.324 296 76 0 1 296 246 541 1.10e-166 474
Msa1453050 AT1G41830.1 71.622 296 84 0 1 296 247 542 2.54e-162 462
Msa1453050 AT4G22010.1 70.169 295 87 1 3 296 247 541 2.48e-151 435
Msa1453050 AT1G21860.1 69.580 286 86 1 3 288 249 533 2.59e-148 427
Msa1453050 AT1G21850.1 68.942 293 90 1 1 293 247 538 3.60e-148 427
Msa1453050 AT4G38420.1 67.450 298 92 2 3 295 252 549 2.11e-140 407
Msa1453050 AT4G28090.1 64.948 291 97 3 1 288 249 537 7.57e-135 393
Msa1453050 AT5G66920.1 55.822 292 129 0 1 292 254 545 1.12e-114 341
Msa1453050 AT4G37160.1 53.559 295 133 1 2 296 251 541 1.81e-106 320
Msa1453050 AT1G55570.1 52.560 293 134 3 3 291 255 546 1.04e-105 318
Msa1453050 AT3G13400.2 53.608 291 131 3 3 290 253 542 2.84e-105 317
Msa1453050 AT3G13400.1 53.608 291 131 3 3 290 253 542 2.84e-105 317
Msa1453050 AT3G13390.1 53.767 292 130 3 3 290 254 544 3.74e-104 315
Msa1453050 AT1G55560.1 52.234 291 135 3 3 290 252 541 1.41e-102 310
Msa1453050 AT2G23630.1 53.147 286 134 0 3 288 250 535 6.66e-102 308
Msa1453050 AT2G23630.2 53.147 286 134 0 3 288 255 540 6.86e-102 308
Msa1453050 AT4G38420.3 65.652 230 74 2 3 227 252 481 1.43e-96 293
Msa1453050 AT4G25240.2 47.619 294 143 7 2 286 260 551 4.47e-87 271
Msa1453050 AT4G25240.1 47.619 294 143 7 2 286 260 551 4.47e-87 271
Msa1453050 AT5G48450.2 47.260 292 146 7 2 287 255 544 3.08e-86 270
Msa1453050 AT5G48450.1 47.260 292 146 7 2 287 255 544 5.92e-86 270
Msa1453050 AT5G51480.1 45.695 302 152 8 2 292 259 559 9.32e-84 263
Msa1453050 AT1G75790.1 46.801 297 147 3 3 288 248 544 4.45e-83 260
Msa1453050 AT4G38420.2 65.517 203 65 2 3 200 252 454 1.03e-82 256
Msa1453050 AT4G12420.1 44.371 302 158 6 2 295 257 556 1.17e-78 250
Msa1453050 AT4G12420.2 44.371 302 158 6 2 295 257 556 1.17e-78 250
Msa1453050 AT4G37160.2 54.271 199 91 0 2 200 251 449 1.13e-68 220
Msa1453050 AT5G21105.2 26.688 311 178 12 4 286 97 385 3.03e-22 95.9
Msa1453050 AT5G21105.1 27.419 310 177 12 4 286 288 576 1.07e-21 95.5
Msa1453050 AT4G39830.3 25.581 301 193 8 8 287 192 482 2.94e-19 87.8
Msa1453050 AT4G39830.4 25.581 301 193 8 8 287 181 471 3.10e-19 87.8
Msa1453050 AT5G21105.3 27.715 267 150 10 4 243 288 538 3.13e-19 88.2
Msa1453050 AT4G39830.2 25.581 301 193 8 8 287 194 484 3.19e-19 87.8
Msa1453050 AT4G39830.1 25.581 301 193 8 8 287 289 579 4.75e-19 87.4
Msa1453050 AT5G58910.1 27.707 314 188 10 4 285 217 523 5.46e-19 87.4
Msa1453050 AT5G58910.2 27.707 314 188 10 4 285 261 567 6.24e-19 87.4
Msa1453050 AT5G58910.3 27.707 314 188 10 4 285 260 566 6.41e-19 87.0
Msa1453050 AT5G21100.1 23.934 305 196 9 4 286 271 561 5.50e-16 78.6
Msa1453050 AT2G38080.1 27.010 311 189 10 4 285 257 558 8.31e-16 77.8
Msa1453050 AT5G01190.1 23.962 313 197 9 4 285 256 558 4.83e-12 66.6
Msa1453050 AT5G01190.2 23.962 313 197 9 4 285 242 544 5.78e-12 66.2
Msa1453050 AT5G09360.1 25.843 267 168 9 4 245 268 529 2.15e-11 64.7

Find 69 sgRNAs with CRISPR-Local

Find 234 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACTGAAGTTTCCAATTATT+TGG 0.084217 tig0034461:+1709 Msa1453050:three_prime_UTR
ACATTGATGGATACTCATTT+TGG 0.174475 tig0034461:+741 Msa1453050:CDS
GTTATTCAAATCCAAATAAT+TGG 0.182004 tig0034461:-1720 None:intergenic
CAACAACCTAGTTATATATT+AGG 0.223346 tig0034461:+1436 Msa1453050:three_prime_UTR
GATCTACCAGAAGGGGTATC+TGG 0.247730 tig0034461:-626 None:intergenic
GGAATGTGAGGAGTGAATTT+TGG 0.258017 tig0034461:+1232 Msa1453050:CDS
CCACAAAGAAGAGCATTCTT+TGG 0.269382 tig0034461:-1333 None:intergenic
CCAAAGAATGCTCTTCTTTG+TGG 0.292466 tig0034461:+1333 Msa1453050:CDS
TTCAAACTTCATTCTCATAA+TGG 0.300039 tig0034461:-1381 None:intergenic
AGAAGAGCATTCTTTGGAAT+TGG 0.303850 tig0034461:-1327 None:intergenic
TTGTATTGGTTACGACTATT+AGG 0.313314 tig0034461:-1006 None:intergenic
ATTCAGATGCTGGATCAATT+AGG 0.328006 tig0034461:+1295 Msa1453050:CDS
TGGTATGATCCTTGTGGATT+TGG 0.339764 tig0034461:-434 None:intergenic
GTCATGGACTGCAATTTATA+TGG 0.346150 tig0034461:+1188 Msa1453050:CDS
AACTTTGGACGATACTTTCA+TGG 0.356230 tig0034461:-715 None:intergenic
CAGCATCTCTTAGGTTGTAT+TGG 0.360857 tig0034461:-1020 None:intergenic
GATCCTTGTGGATTTGGCCT+TGG 0.375319 tig0034461:-428 None:intergenic
TTCAGATGCTGGATCAATTA+GGG 0.377202 tig0034461:+1296 Msa1453050:CDS
GTGAAATGAAGTAGTTCAAT+TGG 0.384829 tig0034461:+1636 Msa1453050:three_prime_UTR
AGCATCCCAGATACCCCTTC+TGG 0.390553 tig0034461:+620 Msa1453050:CDS
CTAATATATAACTAGGTTGT+TGG 0.397045 tig0034461:-1435 None:intergenic
AACAACCTAGTTATATATTA+GGG 0.398056 tig0034461:+1437 Msa1453050:three_prime_UTR
CTAGCTGATTACTTCAAGAT+TGG 0.412868 tig0034461:+578 Msa1453050:CDS
GTCCAAAGTTGGCACATTGA+TGG 0.424178 tig0034461:+728 Msa1453050:CDS
ATCCATCAATGTGCCAACTT+TGG 0.444509 tig0034461:-730 None:intergenic
TCCTAGACACAGCATCTCTT+AGG 0.448380 tig0034461:-1029 None:intergenic
TGGTTAACATTGTGTGGTGT+AGG 0.449159 tig0034461:-1415 None:intergenic
ACAGACGAGTTTGCAAAGAA+TGG 0.452707 tig0034461:-1472 None:intergenic
CTTCTTTGTGGCAAAGCAGT+GGG 0.465661 tig0034461:+1345 Msa1453050:CDS
CCACAAGGATCATACCACTA+TGG 0.467837 tig0034461:+440 Msa1453050:CDS
TTGAATAACTGTGATGTGTT+CGG 0.472674 tig0034461:+1734 Msa1453050:three_prime_UTR
ATTTGTGTAATGCTCGTGTT+TGG 0.478781 tig0034461:-1667 None:intergenic
GCAGAATGGACGGTGGTGTA+TGG 0.483733 tig0034461:+980 Msa1453050:intron
ATCTACCAGAAGGGGTATCT+GGG 0.493408 tig0034461:-625 None:intergenic
CAGACGAGTTTGCAAAGAAT+GGG 0.496723 tig0034461:-1471 None:intergenic
AGCTTCAGAGGAGTGTCAGC+TGG 0.497960 tig0034461:-557 None:intergenic
GCTGATTACTTCAAGATTGG+TGG 0.498079 tig0034461:+581 Msa1453050:CDS
ATGCGAGTATATTCAGATGC+TGG 0.504781 tig0034461:+1285 Msa1453050:CDS
TAAATTGCAGTCCATGACTT+AGG 0.517595 tig0034461:-1183 None:intergenic
AAGTAATCAGCTAGCTTCAG+AGG 0.518399 tig0034461:-569 None:intergenic
AGTTCAGCAGCACAAGTGAA+TGG 0.541477 tig0034461:+497 Msa1453050:CDS
TATCTGGGATGCTACCAACT+TGG 0.549445 tig0034461:-610 None:intergenic
TTATATATTAGGGACTAACT+TGG 0.558841 tig0034461:+1447 Msa1453050:three_prime_UTR
GTACACTGATACTTCAGTCA+TGG 0.561692 tig0034461:+652 Msa1453050:CDS
GGTTGTTGGTTAACATTGTG+TGG 0.563960 tig0034461:-1421 None:intergenic
ATGAAAGTATCGTCCAAAGT+TGG 0.568460 tig0034461:+717 Msa1453050:CDS
TCTTCTTTGTGGCAAAGCAG+TGG 0.568771 tig0034461:+1344 Msa1453050:CDS
TTAGTCCCTAATATATAACT+AGG 0.573736 tig0034461:-1442 None:intergenic
TACACTGATACTTCAGTCAT+GGG 0.574862 tig0034461:+653 Msa1453050:CDS
AGGACAAATCTCACTGCAAG+TGG 0.575670 tig0034461:+404 Msa1453050:intron
ACCTAAGAGATGCTGTGTCT+AGG 0.579180 tig0034461:+1028 Msa1453050:CDS
CCATAGTGGTATGATCCTTG+TGG 0.593975 tig0034461:-440 None:intergenic
TGTACATTGATCTACCAGAA+GGG 0.599518 tig0034461:-634 None:intergenic
CACTAGACAATGTGGGAATG+TGG 0.600172 tig0034461:+1211 Msa1453050:CDS
TAATGTTTATCATACCATAG+TGG 0.606188 tig0034461:-454 None:intergenic
CATTTCTAATGCAGAATGGA+CGG 0.620989 tig0034461:+970 Msa1453050:intron
GTGTACATTGATCTACCAGA+AGG 0.628287 tig0034461:-635 None:intergenic
CTCAGGTATATCCTAAGTCA+TGG 0.631745 tig0034461:+1172 Msa1453050:intron
ATGTGGGAATGTGGAATGTG+AGG 0.633524 tig0034461:+1220 Msa1453050:CDS
TGTGTCTAGGAGCACAACAC+AGG 0.641217 tig0034461:+1041 Msa1453050:CDS
GGACCAAGGCCAAATCCACA+AGG 0.651492 tig0034461:+425 Msa1453050:CDS
AACTTATTATCACAAAGTGA+TGG 0.651762 tig0034461:-1524 None:intergenic
ATCTCACTGCAAGTGGACCA+AGG 0.653008 tig0034461:+411 Msa1453050:CDS
ACACGATCTCAACGAAGGCG+CGG 0.656942 tig0034461:-685 None:intergenic
TTATATGGCACTAGACAATG+TGG 0.668761 tig0034461:+1203 Msa1453050:CDS
GTACATTGATCTACCAGAAG+GGG 0.678351 tig0034461:-633 None:intergenic
CTGAAACACGATCTCAACGA+AGG 0.684228 tig0034461:-690 None:intergenic
TATATGGCACTAGACAATGT+GGG 0.701854 tig0034461:+1204 Msa1453050:CDS
TTCTAATGCAGAATGGACGG+TGG 0.708926 tig0034461:+973 Msa1453050:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTAATTAAATTTCAATCA+TGG + tig0034461:947-966 Msa1453050:intron 10.0%
!! ATTAAAAAATTTCAATAACT+TGG - tig0034461:1144-1163 None:intergenic 10.0%
!! AATTAATTAAATTTCAATCA+TGG + tig0034461:947-966 Msa1453050:intron 10.0%
!! ATTAAAAAATTTCAATAACT+TGG - tig0034461:1144-1163 None:intergenic 10.0%
!!! ATTTCCATCAATTTTTTATC+CGG + tig0034461:797-816 Msa1453050:intron 20.0%
!! TAATGATATTCAAAGATCAT+AGG - tig0034461:1103-1122 None:intergenic 20.0%
!! AACAACCTAGTTATATATTA+GGG + tig0034461:1437-1456 Msa1453050:three_prime_UTR 20.0%
!! GACCAAAAAATAAAACAATA+GGG - tig0034461:1559-1578 None:intergenic 20.0%
!! TGACCAAAAAATAAAACAAT+AGG - tig0034461:1560-1579 None:intergenic 20.0%
!! GTTATTCAAATCCAAATAAT+TGG - tig0034461:1723-1742 None:intergenic 20.0%
!!! ATTTCCATCAATTTTTTATC+CGG + tig0034461:797-816 Msa1453050:intron 20.0%
!! TAATGATATTCAAAGATCAT+AGG - tig0034461:1103-1122 None:intergenic 20.0%
!! AACAACCTAGTTATATATTA+GGG + tig0034461:1437-1456 Msa1453050:three_prime_UTR 20.0%
!! GACCAAAAAATAAAACAATA+GGG - tig0034461:1559-1578 None:intergenic 20.0%
!! TGACCAAAAAATAAAACAAT+AGG - tig0034461:1560-1579 None:intergenic 20.0%
!! GTTATTCAAATCCAAATAAT+TGG - tig0034461:1723-1742 None:intergenic 20.0%
! TAATGTTTATCATACCATAG+TGG - tig0034461:457-476 None:intergenic 25.0%
! TAATCCGGATAAAAAATTGA+TGG - tig0034461:804-823 None:intergenic 25.0%
! TTCAAACTTCATTCTCATAA+TGG - tig0034461:1384-1403 None:intergenic 25.0%
! CTAATATATAACTAGGTTGT+TGG - tig0034461:1438-1457 None:intergenic 25.0%
! CAACAACCTAGTTATATATT+AGG + tig0034461:1436-1455 Msa1453050:three_prime_UTR 25.0%
! TTAGTCCCTAATATATAACT+AGG - tig0034461:1445-1464 None:intergenic 25.0%
! TTATATATTAGGGACTAACT+TGG + tig0034461:1447-1466 Msa1453050:three_prime_UTR 25.0%
! AACTTATTATCACAAAGTGA+TGG - tig0034461:1527-1546 None:intergenic 25.0%
! CAAAAAATAAAACAATAGGG+TGG - tig0034461:1556-1575 None:intergenic 25.0%
!!! CACCCTATTGTTTTATTTTT+TGG + tig0034461:1554-1573 Msa1453050:three_prime_UTR 25.0%
! AACTGAAGTTTCCAATTATT+TGG + tig0034461:1709-1728 Msa1453050:three_prime_UTR 25.0%
! TAATGTTTATCATACCATAG+TGG - tig0034461:457-476 None:intergenic 25.0%
! TAATCCGGATAAAAAATTGA+TGG - tig0034461:804-823 None:intergenic 25.0%
! TTCAAACTTCATTCTCATAA+TGG - tig0034461:1384-1403 None:intergenic 25.0%
! CTAATATATAACTAGGTTGT+TGG - tig0034461:1438-1457 None:intergenic 25.0%
! CAACAACCTAGTTATATATT+AGG + tig0034461:1436-1455 Msa1453050:three_prime_UTR 25.0%
! TTAGTCCCTAATATATAACT+AGG - tig0034461:1445-1464 None:intergenic 25.0%
! TTATATATTAGGGACTAACT+TGG + tig0034461:1447-1466 Msa1453050:three_prime_UTR 25.0%
! AACTTATTATCACAAAGTGA+TGG - tig0034461:1527-1546 None:intergenic 25.0%
! CAAAAAATAAAACAATAGGG+TGG - tig0034461:1556-1575 None:intergenic 25.0%
!!! CACCCTATTGTTTTATTTTT+TGG + tig0034461:1554-1573 Msa1453050:three_prime_UTR 25.0%
! AACTGAAGTTTCCAATTATT+TGG + tig0034461:1709-1728 Msa1453050:three_prime_UTR 25.0%
ATGAACATCTATTGAGTCAT+AGG - tig0034461:9-28 None:intergenic 30.0%
TTCAACTCGTTTCACTAATA+GGG + tig0034461:94-113 Msa1453050:CDS 30.0%
AATTGAACTCAATTACCTGA+TGG - tig0034461:231-250 None:intergenic 30.0%
! GTTCAATTTTTATACACCGT+CGG + tig0034461:243-262 Msa1453050:intron 30.0%
! ATTAAGTTGTTGCAATTCAC+AGG + tig0034461:384-403 Msa1453050:intron 30.0%
!! ACATTGATGGATACTCATTT+TGG + tig0034461:741-760 Msa1453050:CDS 30.0%
!! CATTGATGGATACTCATTTT+GGG + tig0034461:742-761 Msa1453050:CDS 30.0%
TGTAGCATAGTGATTTAATC+CGG - tig0034461:819-838 None:intergenic 30.0%
!! TTGTATTGGTTACGACTATT+AGG - tig0034461:1009-1028 None:intergenic 30.0%
TCATTATACTAGCTAAATGC+AGG + tig0034461:1117-1136 Msa1453050:intron 30.0%
!!! TTTTTTAATTTGTGTGCCTC+AGG + tig0034461:1155-1174 Msa1453050:intron 30.0%
GTGAAATGAAGTAGTTCAAT+TGG + tig0034461:1636-1655 Msa1453050:three_prime_UTR 30.0%
TTGAATAACTGTGATGTGTT+CGG + tig0034461:1734-1753 Msa1453050:three_prime_UTR 30.0%
ATGAACATCTATTGAGTCAT+AGG - tig0034461:9-28 None:intergenic 30.0%
TTCAACTCGTTTCACTAATA+GGG + tig0034461:94-113 Msa1453050:CDS 30.0%
AATTGAACTCAATTACCTGA+TGG - tig0034461:231-250 None:intergenic 30.0%
! GTTCAATTTTTATACACCGT+CGG + tig0034461:243-262 Msa1453050:intron 30.0%
! ATTAAGTTGTTGCAATTCAC+AGG + tig0034461:384-403 Msa1453050:intron 30.0%
!! ACATTGATGGATACTCATTT+TGG + tig0034461:741-760 Msa1453050:CDS 30.0%
!! CATTGATGGATACTCATTTT+GGG + tig0034461:742-761 Msa1453050:CDS 30.0%
TGTAGCATAGTGATTTAATC+CGG - tig0034461:819-838 None:intergenic 30.0%
!! TTGTATTGGTTACGACTATT+AGG - tig0034461:1009-1028 None:intergenic 30.0%
TCATTATACTAGCTAAATGC+AGG + tig0034461:1117-1136 Msa1453050:intron 30.0%
!!! TTTTTTAATTTGTGTGCCTC+AGG + tig0034461:1155-1174 Msa1453050:intron 30.0%
GTGAAATGAAGTAGTTCAAT+TGG + tig0034461:1636-1655 Msa1453050:three_prime_UTR 30.0%
TTGAATAACTGTGATGTGTT+CGG + tig0034461:1734-1753 Msa1453050:three_prime_UTR 30.0%
GACTCAATAGATGTTCATGT+CGG + tig0034461:11-30 Msa1453050:CDS 35.0%
CTTCAACTCGTTTCACTAAT+AGG + tig0034461:93-112 Msa1453050:CDS 35.0%
GATTGGATTATTTAGACCGA+CGG - tig0034461:262-281 None:intergenic 35.0%
ATGAAGAGTCTCTTAGAGAT+TGG - tig0034461:279-298 None:intergenic 35.0%
CTAGCTGATTACTTCAAGAT+TGG + tig0034461:578-597 Msa1453050:CDS 35.0%
TGTACATTGATCTACCAGAA+GGG - tig0034461:637-656 None:intergenic 35.0%
TACACTGATACTTCAGTCAT+GGG + tig0034461:653-672 Msa1453050:CDS 35.0%
AACTTTGGACGATACTTTCA+TGG - tig0034461:718-737 None:intergenic 35.0%
!! ATGAAAGTATCGTCCAAAGT+TGG + tig0034461:717-736 Msa1453050:CDS 35.0%
ATGGCATTTCTAATGCAGAA+TGG + tig0034461:966-985 Msa1453050:intron 35.0%
CATTTCTAATGCAGAATGGA+CGG + tig0034461:970-989 Msa1453050:intron 35.0%
TAAATTGCAGTCCATGACTT+AGG - tig0034461:1186-1205 None:intergenic 35.0%
! GTCATGGACTGCAATTTATA+TGG + tig0034461:1188-1207 Msa1453050:CDS 35.0%
TTATATGGCACTAGACAATG+TGG + tig0034461:1203-1222 Msa1453050:CDS 35.0%
TATATGGCACTAGACAATGT+GGG + tig0034461:1204-1223 Msa1453050:CDS 35.0%
! GAATGTGAGGAGTGAATTTT+GGG + tig0034461:1233-1252 Msa1453050:CDS 35.0%
ATTCAGATGCTGGATCAATT+AGG + tig0034461:1295-1314 Msa1453050:CDS 35.0%
TTCAGATGCTGGATCAATTA+GGG + tig0034461:1296-1315 Msa1453050:CDS 35.0%
!! AGAAGAGCATTCTTTGGAAT+TGG - tig0034461:1330-1349 None:intergenic 35.0%
ATTTGTGTAATGCTCGTGTT+TGG - tig0034461:1670-1689 None:intergenic 35.0%
GACTCAATAGATGTTCATGT+CGG + tig0034461:11-30 Msa1453050:CDS 35.0%
CTTCAACTCGTTTCACTAAT+AGG + tig0034461:93-112 Msa1453050:CDS 35.0%
GATTGGATTATTTAGACCGA+CGG - tig0034461:262-281 None:intergenic 35.0%
ATGAAGAGTCTCTTAGAGAT+TGG - tig0034461:279-298 None:intergenic 35.0%
CTAGCTGATTACTTCAAGAT+TGG + tig0034461:578-597 Msa1453050:CDS 35.0%
TGTACATTGATCTACCAGAA+GGG - tig0034461:637-656 None:intergenic 35.0%
TACACTGATACTTCAGTCAT+GGG + tig0034461:653-672 Msa1453050:CDS 35.0%
AACTTTGGACGATACTTTCA+TGG - tig0034461:718-737 None:intergenic 35.0%
!! ATGAAAGTATCGTCCAAAGT+TGG + tig0034461:717-736 Msa1453050:CDS 35.0%
ATGGCATTTCTAATGCAGAA+TGG + tig0034461:966-985 Msa1453050:intron 35.0%
CATTTCTAATGCAGAATGGA+CGG + tig0034461:970-989 Msa1453050:intron 35.0%
TAAATTGCAGTCCATGACTT+AGG - tig0034461:1186-1205 None:intergenic 35.0%
! GTCATGGACTGCAATTTATA+TGG + tig0034461:1188-1207 Msa1453050:CDS 35.0%
TTATATGGCACTAGACAATG+TGG + tig0034461:1203-1222 Msa1453050:CDS 35.0%
TATATGGCACTAGACAATGT+GGG + tig0034461:1204-1223 Msa1453050:CDS 35.0%
! GAATGTGAGGAGTGAATTTT+GGG + tig0034461:1233-1252 Msa1453050:CDS 35.0%
ATTCAGATGCTGGATCAATT+AGG + tig0034461:1295-1314 Msa1453050:CDS 35.0%
TTCAGATGCTGGATCAATTA+GGG + tig0034461:1296-1315 Msa1453050:CDS 35.0%
!! AGAAGAGCATTCTTTGGAAT+TGG - tig0034461:1330-1349 None:intergenic 35.0%
ATTTGTGTAATGCTCGTGTT+TGG - tig0034461:1670-1689 None:intergenic 35.0%
GCAACAATGTAGTAGTCCTT+CGG - tig0034461:74-93 None:intergenic 40.0%
AACTCACAACAACCTCTTTC+AGG + tig0034461:149-168 Msa1453050:CDS 40.0%
TGGTATGATCCTTGTGGATT+TGG - tig0034461:437-456 None:intergenic 40.0%
! AAGTAATCAGCTAGCTTCAG+AGG - tig0034461:572-591 None:intergenic 40.0%
GCTGATTACTTCAAGATTGG+TGG + tig0034461:581-600 Msa1453050:CDS 40.0%
!!! ATTGGTGGTGTTTTCCAAGT+TGG + tig0034461:596-615 Msa1453050:CDS 40.0%
GTACATTGATCTACCAGAAG+GGG - tig0034461:636-655 None:intergenic 40.0%
GTGTACATTGATCTACCAGA+AGG - tig0034461:638-657 None:intergenic 40.0%
GTACACTGATACTTCAGTCA+TGG + tig0034461:652-671 Msa1453050:CDS 40.0%
ATCCATCAATGTGCCAACTT+TGG - tig0034461:733-752 None:intergenic 40.0%
!!! GGATACTCATTTTGGGTTGT+CGG + tig0034461:749-768 Msa1453050:CDS 40.0%
!!! GATACTCATTTTGGGTTGTC+GGG + tig0034461:750-769 Msa1453050:CDS 40.0%
! CAGCATCTCTTAGGTTGTAT+TGG - tig0034461:1023-1042 None:intergenic 40.0%
CATGACTTAGGATATACCTG+AGG - tig0034461:1174-1193 None:intergenic 40.0%
CTCAGGTATATCCTAAGTCA+TGG + tig0034461:1172-1191 Msa1453050:intron 40.0%
! GGAATGTGAGGAGTGAATTT+TGG + tig0034461:1232-1251 Msa1453050:CDS 40.0%
! TTTTGGGCACGACAATATCT+TGG + tig0034461:1249-1268 Msa1453050:CDS 40.0%
ATGCGAGTATATTCAGATGC+TGG + tig0034461:1285-1304 Msa1453050:CDS 40.0%
!! CCACAAAGAAGAGCATTCTT+TGG - tig0034461:1336-1355 None:intergenic 40.0%
CCAAAGAATGCTCTTCTTTG+TGG + tig0034461:1333-1352 Msa1453050:CDS 40.0%
TGGTTAACATTGTGTGGTGT+AGG - tig0034461:1418-1437 None:intergenic 40.0%
! GGTTGTTGGTTAACATTGTG+TGG - tig0034461:1424-1443 None:intergenic 40.0%
CAGACGAGTTTGCAAAGAAT+GGG - tig0034461:1474-1493 None:intergenic 40.0%
ACAGACGAGTTTGCAAAGAA+TGG - tig0034461:1475-1494 None:intergenic 40.0%
GCAACAATGTAGTAGTCCTT+CGG - tig0034461:74-93 None:intergenic 40.0%
AACTCACAACAACCTCTTTC+AGG + tig0034461:149-168 Msa1453050:CDS 40.0%
TGGTATGATCCTTGTGGATT+TGG - tig0034461:437-456 None:intergenic 40.0%
! AAGTAATCAGCTAGCTTCAG+AGG - tig0034461:572-591 None:intergenic 40.0%
GCTGATTACTTCAAGATTGG+TGG + tig0034461:581-600 Msa1453050:CDS 40.0%
!!! ATTGGTGGTGTTTTCCAAGT+TGG + tig0034461:596-615 Msa1453050:CDS 40.0%
GTACATTGATCTACCAGAAG+GGG - tig0034461:636-655 None:intergenic 40.0%
GTGTACATTGATCTACCAGA+AGG - tig0034461:638-657 None:intergenic 40.0%
GTACACTGATACTTCAGTCA+TGG + tig0034461:652-671 Msa1453050:CDS 40.0%
ATCCATCAATGTGCCAACTT+TGG - tig0034461:733-752 None:intergenic 40.0%
!!! GGATACTCATTTTGGGTTGT+CGG + tig0034461:749-768 Msa1453050:CDS 40.0%
!!! GATACTCATTTTGGGTTGTC+GGG + tig0034461:750-769 Msa1453050:CDS 40.0%
! CAGCATCTCTTAGGTTGTAT+TGG - tig0034461:1023-1042 None:intergenic 40.0%
CATGACTTAGGATATACCTG+AGG - tig0034461:1174-1193 None:intergenic 40.0%
CTCAGGTATATCCTAAGTCA+TGG + tig0034461:1172-1191 Msa1453050:intron 40.0%
! GGAATGTGAGGAGTGAATTT+TGG + tig0034461:1232-1251 Msa1453050:CDS 40.0%
! TTTTGGGCACGACAATATCT+TGG + tig0034461:1249-1268 Msa1453050:CDS 40.0%
ATGCGAGTATATTCAGATGC+TGG + tig0034461:1285-1304 Msa1453050:CDS 40.0%
!! CCACAAAGAAGAGCATTCTT+TGG - tig0034461:1336-1355 None:intergenic 40.0%
CCAAAGAATGCTCTTCTTTG+TGG + tig0034461:1333-1352 Msa1453050:CDS 40.0%
TGGTTAACATTGTGTGGTGT+AGG - tig0034461:1418-1437 None:intergenic 40.0%
! GGTTGTTGGTTAACATTGTG+TGG - tig0034461:1424-1443 None:intergenic 40.0%
CAGACGAGTTTGCAAAGAAT+GGG - tig0034461:1474-1493 None:intergenic 40.0%
ACAGACGAGTTTGCAAAGAA+TGG - tig0034461:1475-1494 None:intergenic 40.0%
CTGATCAGCTGTGATAAGCA+CGG - tig0034461:48-67 None:intergenic 45.0%
!! GATGGATCTAGCTTGTTGGA+TGG - tig0034461:213-232 None:intergenic 45.0%
TCCAACAAGCTAGATCCATC+AGG + tig0034461:213-232 Msa1453050:CDS 45.0%
! ACCTGATGGATCTAGCTTGT+TGG - tig0034461:217-236 None:intergenic 45.0%
AGGACAAATCTCACTGCAAG+TGG + tig0034461:404-423 Msa1453050:intron 45.0%
CCATAGTGGTATGATCCTTG+TGG - tig0034461:443-462 None:intergenic 45.0%
CCACAAGGATCATACCACTA+TGG + tig0034461:440-459 Msa1453050:CDS 45.0%
AGTTCAGCAGCACAAGTGAA+TGG + tig0034461:497-516 Msa1453050:CDS 45.0%
! TATCTGGGATGCTACCAACT+TGG - tig0034461:613-632 None:intergenic 45.0%
!! ATCTACCAGAAGGGGTATCT+GGG - tig0034461:628-647 None:intergenic 45.0%
CTGAAACACGATCTCAACGA+AGG - tig0034461:693-712 None:intergenic 45.0%
GTCCAAAGTTGGCACATTGA+TGG + tig0034461:728-747 Msa1453050:CDS 45.0%
TTCTAATGCAGAATGGACGG+TGG + tig0034461:973-992 Msa1453050:intron 45.0%
ACCTAAGAGATGCTGTGTCT+AGG + tig0034461:1028-1047 Msa1453050:CDS 45.0%
TCCTAGACACAGCATCTCTT+AGG - tig0034461:1032-1051 None:intergenic 45.0%
CACTAGACAATGTGGGAATG+TGG + tig0034461:1211-1230 Msa1453050:CDS 45.0%
ATGTGGGAATGTGGAATGTG+AGG + tig0034461:1220-1239 Msa1453050:CDS 45.0%
TCTTCTTTGTGGCAAAGCAG+TGG + tig0034461:1344-1363 Msa1453050:CDS 45.0%
CTTCTTTGTGGCAAAGCAGT+GGG + tig0034461:1345-1364 Msa1453050:CDS 45.0%
CTGATCAGCTGTGATAAGCA+CGG - tig0034461:48-67 None:intergenic 45.0%
!! GATGGATCTAGCTTGTTGGA+TGG - tig0034461:213-232 None:intergenic 45.0%
TCCAACAAGCTAGATCCATC+AGG + tig0034461:213-232 Msa1453050:CDS 45.0%
! ACCTGATGGATCTAGCTTGT+TGG - tig0034461:217-236 None:intergenic 45.0%
AGGACAAATCTCACTGCAAG+TGG + tig0034461:404-423 Msa1453050:intron 45.0%
CCATAGTGGTATGATCCTTG+TGG - tig0034461:443-462 None:intergenic 45.0%
CCACAAGGATCATACCACTA+TGG + tig0034461:440-459 Msa1453050:CDS 45.0%
AGTTCAGCAGCACAAGTGAA+TGG + tig0034461:497-516 Msa1453050:CDS 45.0%
! TATCTGGGATGCTACCAACT+TGG - tig0034461:613-632 None:intergenic 45.0%
!! ATCTACCAGAAGGGGTATCT+GGG - tig0034461:628-647 None:intergenic 45.0%
CTGAAACACGATCTCAACGA+AGG - tig0034461:693-712 None:intergenic 45.0%
GTCCAAAGTTGGCACATTGA+TGG + tig0034461:728-747 Msa1453050:CDS 45.0%
TTCTAATGCAGAATGGACGG+TGG + tig0034461:973-992 Msa1453050:intron 45.0%
ACCTAAGAGATGCTGTGTCT+AGG + tig0034461:1028-1047 Msa1453050:CDS 45.0%
TCCTAGACACAGCATCTCTT+AGG - tig0034461:1032-1051 None:intergenic 45.0%
CACTAGACAATGTGGGAATG+TGG + tig0034461:1211-1230 Msa1453050:CDS 45.0%
ATGTGGGAATGTGGAATGTG+AGG + tig0034461:1220-1239 Msa1453050:CDS 45.0%
TCTTCTTTGTGGCAAAGCAG+TGG + tig0034461:1344-1363 Msa1453050:CDS 45.0%
CTTCTTTGTGGCAAAGCAGT+GGG + tig0034461:1345-1364 Msa1453050:CDS 45.0%
CGTGCTTATCACAGCTGATC+AGG + tig0034461:46-65 Msa1453050:CDS 50.0%
GACCAATCGATCTGAGTGGT+CGG - tig0034461:191-210 None:intergenic 50.0%
GATGGACCAATCGATCTGAG+TGG - tig0034461:195-214 None:intergenic 50.0%
ATCTCACTGCAAGTGGACCA+AGG + tig0034461:411-430 Msa1453050:CDS 50.0%
GATCCTTGTGGATTTGGCCT+TGG - tig0034461:431-450 None:intergenic 50.0%
!! GATCTACCAGAAGGGGTATC+TGG - tig0034461:629-648 None:intergenic 50.0%
! TGTGTCTAGGAGCACAACAC+AGG + tig0034461:1041-1060 Msa1453050:CDS 50.0%
CGTGCTTATCACAGCTGATC+AGG + tig0034461:46-65 Msa1453050:CDS 50.0%
GACCAATCGATCTGAGTGGT+CGG - tig0034461:191-210 None:intergenic 50.0%
GATGGACCAATCGATCTGAG+TGG - tig0034461:195-214 None:intergenic 50.0%
ATCTCACTGCAAGTGGACCA+AGG + tig0034461:411-430 Msa1453050:CDS 50.0%
GATCCTTGTGGATTTGGCCT+TGG - tig0034461:431-450 None:intergenic 50.0%
!! GATCTACCAGAAGGGGTATC+TGG - tig0034461:629-648 None:intergenic 50.0%
! TGTGTCTAGGAGCACAACAC+AGG + tig0034461:1041-1060 Msa1453050:CDS 50.0%
GACCGACCACTCAGATCGAT+TGG + tig0034461:186-205 Msa1453050:CDS 55.0%
GGACCAAGGCCAAATCCACA+AGG + tig0034461:425-444 Msa1453050:CDS 55.0%
!! AGCTTCAGAGGAGTGTCAGC+TGG - tig0034461:560-579 None:intergenic 55.0%
AGCATCCCAGATACCCCTTC+TGG + tig0034461:620-639 Msa1453050:CDS 55.0%
ACACGATCTCAACGAAGGCG+CGG - tig0034461:688-707 None:intergenic 55.0%
GCAGAATGGACGGTGGTGTA+TGG + tig0034461:980-999 Msa1453050:intron 55.0%
GACCGACCACTCAGATCGAT+TGG + tig0034461:186-205 Msa1453050:CDS 55.0%
GGACCAAGGCCAAATCCACA+AGG + tig0034461:425-444 Msa1453050:CDS 55.0%
!! AGCTTCAGAGGAGTGTCAGC+TGG - tig0034461:560-579 None:intergenic 55.0%
AGCATCCCAGATACCCCTTC+TGG + tig0034461:620-639 Msa1453050:CDS 55.0%
ACACGATCTCAACGAAGGCG+CGG - tig0034461:688-707 None:intergenic 55.0%
GCAGAATGGACGGTGGTGTA+TGG + tig0034461:980-999 Msa1453050:intron 55.0%
!! CACAGCTGATCAGGCACCGA+AGG + tig0034461:55-74 Msa1453050:CDS 60.0%
CCTCTTTCAGGCCCCATTCC+CGG + tig0034461:161-180 Msa1453050:CDS 60.0%
ATCTGAGTGGTCGGTCCACC+GGG - tig0034461:182-201 None:intergenic 60.0%
GATCTGAGTGGTCGGTCCAC+CGG - tig0034461:183-202 None:intergenic 60.0%
!! CACAGCTGATCAGGCACCGA+AGG + tig0034461:55-74 Msa1453050:CDS 60.0%
CCTCTTTCAGGCCCCATTCC+CGG + tig0034461:161-180 Msa1453050:CDS 60.0%
ATCTGAGTGGTCGGTCCACC+GGG - tig0034461:182-201 None:intergenic 60.0%
GATCTGAGTGGTCGGTCCAC+CGG - tig0034461:183-202 None:intergenic 60.0%
CCGGGAATGGGGCCTGAAAG+AGG - tig0034461:164-183 None:intergenic 65.0%
CTTTCAGGCCCCATTCCCGG+TGG + tig0034461:164-183 Msa1453050:CDS 65.0%
TGGTCGGTCCACCGGGAATG+GGG - tig0034461:175-194 None:intergenic 65.0%
GTGGTCGGTCCACCGGGAAT+GGG - tig0034461:176-195 None:intergenic 65.0%
AGTGGTCGGTCCACCGGGAA+TGG - tig0034461:177-196 None:intergenic 65.0%
CCGGGAATGGGGCCTGAAAG+AGG - tig0034461:164-183 None:intergenic 65.0%
CTTTCAGGCCCCATTCCCGG+TGG + tig0034461:164-183 Msa1453050:CDS 65.0%
TGGTCGGTCCACCGGGAATG+GGG - tig0034461:175-194 None:intergenic 65.0%
GTGGTCGGTCCACCGGGAAT+GGG - tig0034461:176-195 None:intergenic 65.0%
AGTGGTCGGTCCACCGGGAA+TGG - tig0034461:177-196 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
tig0034461 gene 2 1768 2 ID=Msa1453050;Name=Msa1453050
tig0034461 mRNA 2 1768 2 ID=Msa1453050-mRNA-1;Parent=Msa1453050;Name=Msa1453050-mRNA-1;_AED=0.13;_eAED=0.13;_QI=0|1|0.75|1|1|1|4|379|296
tig0034461 exon 2 234 2 ID=Msa1453050-mRNA-1:exon:18983;Parent=Msa1453050-mRNA-1
tig0034461 exon 406 771 406 ID=Msa1453050-mRNA-1:exon:18984;Parent=Msa1453050-mRNA-1
tig0034461 exon 984 1062 984 ID=Msa1453050-mRNA-1:exon:18985;Parent=Msa1453050-mRNA-1
tig0034461 exon 1177 1768 1177 ID=Msa1453050-mRNA-1:exon:18986;Parent=Msa1453050-mRNA-1
tig0034461 CDS 2 234 2 ID=Msa1453050-mRNA-1:cds;Parent=Msa1453050-mRNA-1
tig0034461 CDS 406 771 406 ID=Msa1453050-mRNA-1:cds;Parent=Msa1453050-mRNA-1
tig0034461 CDS 984 1062 984 ID=Msa1453050-mRNA-1:cds;Parent=Msa1453050-mRNA-1
tig0034461 CDS 1177 1389 1177 ID=Msa1453050-mRNA-1:cds;Parent=Msa1453050-mRNA-1
tig0034461 three_prime_UTR 1390 1768 1390 ID=Msa1453050-mRNA-1:three_prime_utr;Parent=Msa1453050-mRNA-1
Gene Sequence

>Msa1453050

ACATCCTATGACTCAATAGATGTTCATGTCGGACAATCTTACTCCGTGCTTATCACAGCTGATCAGGCACCGAAGGACTACTACATTGTTGCTTCAACTCGTTTCACTAATAGGGTTCTCACAAGCACTGCAGCACTTCACTACAGTAACTCACAACAACCTCTTTCAGGCCCCATTCCCGGTGGACCGACCACTCAGATCGATTGGTCCATCCAACAAGCTAGATCCATCAGGACAAATCTCACTGCAAGTGGACCAAGGCCAAATCCACAAGGATCATACCACTATGGTATGATAAACATTAGCAGAACAATTAAGCTTGAGAGTTCAGCAGCACAAGTGAATGGCAAGCAAAGATATGCAGTCAACAGTGTATCTTTCATACCAGCTGACACTCCTCTGAAGCTAGCTGATTACTTCAAGATTGGTGGTGTTTTCCAAGTTGGTAGCATCCCAGATACCCCTTCTGGTAGATCAATGTACACTGATACTTCAGTCATGGGTGCTGATTTCCGCGCCTTCGTTGAGATCGTGTTTCAGAACCATGAAAGTATCGTCCAAAGTTGGCACATTGATGGATACTCATTTTGGGTTGTCGGAATGGACGGTGGTGTATGGACGCCTAATAGTCGTAACCAATACAACCTAAGAGATGCTGTGTCTAGGAGCACAACACAGGTATATCCTAAGTCATGGACTGCAATTTATATGGCACTAGACAATGTGGGAATGTGGAATGTGAGGAGTGAATTTTGGGCACGACAATATCTTGGACAACAATTCTATATGCGAGTATATTCAGATGCTGGATCAATTAGGGATGAATATCCAATTCCAAAGAATGCTCTTCTTTGTGGCAAAGCAGTGGGCAGAGAAACTAGACCATTATGA
Protein sequence

>Msa1453050

TSYDSIDVHVGQSYSVLITADQAPKDYYIVASTRFTNRVLTSTAALHYSNSQQPLSGPIPGGPTTQIDWSIQQARSIRTNLTASGPRPNPQGSYHYGMINISRTIKLESSAAQVNGKQRYAVNSVSFIPADTPLKLADYFKIGGVFQVGSIPDTPSGRSMYTDTSVMGADFRAFVEIVFQNHESIVQSWHIDGYSFWVVGMDGGVWTPNSRNQYNLRDAVSRSTTQVYPKSWTAIYMALDNVGMWNVRSEFWARQYLGQQFYMRVYSDAGSIRDEYPIPKNALLCGKAVGRETRPL*