AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0566340

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0566340 MtrunA17_Chr8g0380901 99.174 121 1 0 1 121 1 121 9.61e-88 250
Msa0566340 MtrunA17_Chr8g0380711 77.869 122 26 1 1 121 1 122 1.08e-56 172
Msa0566340 MtrunA17_Chr3g0125791 60.800 125 44 1 1 120 1 125 1.52e-51 159
Msa0566340 MtrunA17_Chr3g0125761 59.843 127 44 2 1 121 1 126 8.59e-51 157
Msa0566340 MtrunA17_Chr2g0301431 54.839 124 52 1 1 120 1 124 2.32e-45 143
Msa0566340 MtrunA17_Chr2g0323021 40.870 115 63 4 9 119 6 119 9.91e-25 94.0
Msa0566340 MtrunA17_Chr7g0253681 39.837 123 69 4 3 120 1 123 1.36e-22 86.7
Msa0566340 MtrunA17_Chr7g0253701 36.885 122 73 3 3 120 1 122 1.09e-21 84.3
Msa0566340 MtrunA17_Chr7g0253671 36.885 122 73 3 3 120 1 122 1.09e-21 84.3
Msa0566340 MtrunA17_Chr1g0177341 40.171 117 66 3 7 119 4 120 1.65e-21 83.6
Msa0566340 MtrunA17_Chr1g0177331 40.171 117 66 3 7 119 4 120 1.65e-21 83.6
Msa0566340 MtrunA17_Chr7g0253731 39.344 122 67 4 1 118 1 119 4.08e-21 82.8
Msa0566340 MtrunA17_Chr1g0205701 34.483 116 67 3 11 119 8 121 1.45e-20 82.0
Msa0566340 MtrunA17_Chr7g0253761 39.344 122 69 3 1 119 1 120 4.89e-20 80.5
Msa0566340 MtrunA17_Chr8g0335531 41.379 116 60 5 11 119 5 119 1.08e-19 79.7
Msa0566340 MtrunA17_Chr5g0394321 33.871 124 75 3 1 119 1 122 1.09e-19 79.7
Msa0566340 MtrunA17_Chr7g0253641 38.525 122 70 3 1 119 1 120 1.63e-19 80.1
Msa0566340 MtrunA17_Chr1g0211101 35.897 117 69 4 8 120 15 129 2.07e-18 76.3
Msa0566340 MtrunA17_Chr8g0335571 38.793 116 63 5 11 119 5 119 5.18e-18 75.9
Msa0566340 MtrunA17_Chr4g0064041 40.000 100 53 2 27 119 190 289 9.52e-18 77.4
Msa0566340 MtrunA17_Chr4g0064041 35.537 121 67 3 10 119 7 127 2.28e-15 70.5
Msa0566340 MtrunA17_Chr6g0481491 36.170 94 56 3 28 118 28 120 3.02e-17 73.9
Msa0566340 MtrunA17_Chr2g0306461 33.065 124 76 3 1 118 1 123 5.67e-17 73.2
Msa0566340 MtrunA17_Chr6g0483981 38.525 122 64 3 6 118 7 126 7.28e-17 75.1
Msa0566340 MtrunA17_Chr6g0459441 37.000 100 58 4 25 120 23 121 4.41e-16 70.1
Msa0566340 MtrunA17_Chr3g0132701 38.596 114 64 5 11 118 7 120 1.35e-15 69.7
Msa0566340 MtrunA17_Chr7g0253631 37.705 122 71 3 1 119 1 120 2.14e-15 69.7
Msa0566340 MtrunA17_Chr3g0132431 38.793 116 61 6 11 119 7 119 3.33e-15 68.2
Msa0566340 MtrunA17_Chr3g0132511 37.391 115 66 5 11 119 7 121 1.62e-14 67.0
Msa0566340 MtrunA17_Chr6g0453801 30.952 126 79 3 1 120 8 131 2.84e-14 65.5
Msa0566340 MtrunA17_Chr6g0453921 33.613 119 74 3 7 120 16 134 4.55e-13 62.4
Msa0566340 MtrunA17_Chr8g0387804 32.759 116 64 4 13 120 18 127 7.60e-13 60.8
Msa0566340 MtrunA17_Chr3g0132411 36.752 117 68 5 7 118 42 157 1.01e-12 61.2
Msa0566340 MtrunA17_Chr4g0033131 25.385 130 88 2 1 121 1 130 1.03e-12 61.2
Msa0566340 MtrunA17_Chr4g0033531 25.385 130 88 2 1 121 1 130 1.03e-12 61.2
Msa0566340 MtrunA17_Chr1g0150131 31.897 116 75 2 7 118 8 123 2.33e-12 60.5
Msa0566340 MtrunA17_Chr8g0360371 31.304 115 74 2 9 118 5 119 3.84e-12 61.2
Msa0566340 MtrunA17_Chr4g0064031 33.019 106 59 4 21 115 21 125 1.19e-11 58.2
Msa0566340 MtrunA17_Chr4g0001571 31.250 96 64 1 25 118 26 121 1.40e-11 58.2
Msa0566340 MtrunA17_Chr6g0453791 32.292 96 62 2 28 120 31 126 8.01e-11 56.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0566340 AT2G02850.1 58.594 128 45 1 1 120 1 128 1.17e-50 157
Msa0566340 AT3G17675.1 36.066 122 73 3 5 121 7 128 9.14e-22 84.0
Msa0566340 AT2G32300.1 37.391 115 66 3 11 119 6 120 2.79e-20 83.2
Msa0566340 AT2G32300.2 37.391 115 66 3 11 119 36 150 4.61e-20 83.2
Msa0566340 AT1G17800.1 39.496 119 66 4 8 120 18 136 5.94e-20 79.7
Msa0566340 AT3G60270.1 42.857 98 52 3 25 119 23 119 6.27e-20 80.9
Msa0566340 AT5G26330.1 37.168 113 65 3 12 119 9 120 1.95e-19 79.3
Msa0566340 AT3G27200.1 36.134 119 71 4 7 121 5 122 4.50e-19 78.2
Msa0566340 AT3G60280.1 44.898 98 48 4 25 118 21 116 6.14e-18 76.3
Msa0566340 AT2G31050.1 33.858 127 73 4 1 119 1 124 3.89e-17 73.9
Msa0566340 AT2G26720.1 32.743 113 72 2 12 120 13 125 7.58e-17 73.2
Msa0566340 AT2G44790.1 43.137 102 48 5 23 119 27 123 1.42e-16 72.4
Msa0566340 AT5G07475.1 34.821 112 69 3 11 119 13 123 6.20e-16 70.5
Msa0566340 AT2G25060.1 36.190 105 59 3 22 118 25 129 1.53e-13 63.9
Msa0566340 AT3G20570.1 31.858 113 70 3 12 118 15 126 4.23e-13 63.2
Msa0566340 AT1G48940.1 33.077 130 65 8 6 120 3 125 1.82e-12 61.2
Msa0566340 AT4G12880.1 30.081 123 79 4 3 118 1 123 3.99e-12 59.3
Msa0566340 AT4G27520.1 33.913 115 67 3 9 118 16 126 1.05e-11 60.5
Msa0566340 AT3G18590.1 31.148 122 69 7 9 120 10 126 1.13e-11 59.3
Msa0566340 AT2G27035.1 30.508 118 76 3 8 120 8 124 1.18e-11 58.5
Msa0566340 AT1G22480.1 35.965 114 67 4 11 121 4 114 1.47e-11 58.5
Msa0566340 AT4G31840.1 28.814 118 76 3 9 118 8 125 1.61e-11 58.5
Msa0566340 AT1G72230.1 33.628 113 71 3 9 118 4 115 2.00e-11 58.5
Msa0566340 AT3G53330.1 32.039 103 64 3 22 118 182 284 4.45e-11 58.9

Find 74 sgRNAs with CRISPR-Local

Find 168 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTATCCACTTATTTAATTTA+AGG 0.058031 4_1:+67403999 None:intergenic
TAATTGTGTAATATGATTTC+TGG 0.140908 4_1:-67403909 Msa0566340:three_prime_UTR
ATTTACCACTGTTATTATTT+TGG 0.148598 4_1:-67403827 Msa0566340:three_prime_UTR
GATTTGAAGGTAAGTGAATT+TGG 0.221004 4_1:+67404823 None:intergenic
AAATATAAACATAATGAGTT+TGG 0.254292 4_1:-67404984 Msa0566340:five_prime_UTR
AAGTTGAACACTACATTATT+AGG 0.282780 4_1:+67404104 None:intergenic
GGCCTAATGGAAAACGCTTT+AGG 0.303848 4_1:-67404632 Msa0566340:CDS
TAAATACTTCTTCATATTGC+AGG 0.322954 4_1:+67404929 None:intergenic
ACCTACACTGTTGGAGGTCC+TGG 0.340570 4_1:-67404681 Msa0566340:CDS
AGGCCACCCAACAGTGTTAA+AGG 0.340614 4_1:+67404650 None:intergenic
GCCTAATGGAAAACGCTTTA+GGG 0.345587 4_1:-67404631 Msa0566340:CDS
AACACTGTTGGGTGGCCTAA+TGG 0.351296 4_1:-67404645 Msa0566340:CDS
CACCCATTAAGATTTCTTGC+AGG 0.368254 4_1:-67404893 Msa0566340:five_prime_UTR
TAAAAGTTAAGCAGCATTGA+TGG 0.375502 4_1:+67404146 None:intergenic
GTAGTTCCCTTCAAAACAAT+TGG 0.383981 4_1:+67403861 None:intergenic
AGGCCTGCAAGAAATCTTAA+TGG 0.392067 4_1:+67404890 None:intergenic
TTATCCTTAAATTAAATAAG+TGG 0.400701 4_1:-67404003 Msa0566340:three_prime_UTR
CTACTTCATTTGCAACTTTG+TGG 0.404030 4_1:-67404196 Msa0566340:CDS
GTTGGACCTTTAACACTGTT+GGG 0.416893 4_1:-67404656 Msa0566340:CDS
AATTGTGTAATATGATTTCT+GGG 0.417127 4_1:-67403908 Msa0566340:three_prime_UTR
CTACTATATGTTAGTCATCA+TGG 0.429365 4_1:-67403944 Msa0566340:three_prime_UTR
AATATAAACATAATGAGTTT+GGG 0.430122 4_1:-67404983 Msa0566340:five_prime_UTR
TGTGAAAGAAAGGGATTTGA+AGG 0.430605 4_1:+67404810 None:intergenic
GCTAGAAAACTGTGAAAGAA+AGG 0.441460 4_1:+67404800 None:intergenic
TACTTCATTTGCAACTTTGT+GGG 0.443056 4_1:-67404195 Msa0566340:CDS
AAGATCAGATCAGGCTTGCT+AGG 0.444325 4_1:-67404227 Msa0566340:CDS
ATATGATTTCTGGGTCTCTC+AGG 0.449735 4_1:-67403899 Msa0566340:three_prime_UTR
TTTGTGGGTCACTGCGAGTC+CGG 0.457246 4_1:-67404180 Msa0566340:CDS
CTAGTAGAGAAACATGGCTC+TGG 0.459629 4_1:-67404772 Msa0566340:exon
GAATTTGGAATGACGTGGTA+AGG 0.466552 4_1:+67404838 None:intergenic
TTCAAGTTCACATAAAGAAC+AGG 0.477674 4_1:+67404042 None:intergenic
TTGGGCAGACCCACGTGTCT+TGG 0.480551 4_1:-67404965 Msa0566340:five_prime_UTR
CTAGAAAACTGTGAAAGAAA+GGG 0.482984 4_1:+67404801 None:intergenic
CAAGAGGAGCAAAAGTGTAT+AGG 0.485165 4_1:-67404257 Msa0566340:CDS
GGTTGGACCTTTAACACTGT+TGG 0.487153 4_1:-67404657 Msa0566340:CDS
GGACAGGAAAAGATCAGATC+AGG 0.488069 4_1:-67404236 Msa0566340:CDS
AGAAACATGGCTCTGGGAAG+AGG 0.491666 4_1:-67404765 Msa0566340:exon
GGAGCAAAAGTGTATAGGAC+AGG 0.499425 4_1:-67404252 Msa0566340:CDS
GGCCTGCAAGAAATCTTAAT+GGG 0.503092 4_1:+67404891 None:intergenic
GTGTTAAAGGTCCAACCTCC+AGG 0.508532 4_1:+67404663 None:intergenic
GCTGCTACCTACACTGTTGG+AGG 0.517016 4_1:-67404687 Msa0566340:CDS
CATGCTGCTACCTACACTGT+TGG 0.528640 4_1:-67404690 Msa0566340:CDS
AGATCAGATCAGGCTTGCTA+GGG 0.531401 4_1:-67404226 Msa0566340:CDS
CTGTTGGAGGTCCTGGAGGT+TGG 0.534102 4_1:-67404674 Msa0566340:CDS
TTTAGGGCTGGTGACACACT+TGG 0.537054 4_1:-67404615 Msa0566340:intron
AAGAAATCTTAATGGGTGGT+TGG 0.538997 4_1:+67404898 None:intergenic
TCTGGGAAGAGGCAGTGCAT+TGG 0.544752 4_1:-67404754 Msa0566340:CDS
AAATTAAATAAGTGGATAGG+TGG 0.544930 4_1:-67403995 Msa0566340:three_prime_UTR
AATGGAAAACGCTTTAGGGC+TGG 0.547621 4_1:-67404627 Msa0566340:CDS
GCCACCACATTGTGAGCTGA+TGG 0.559088 4_1:+67404315 None:intergenic
GGACCTTTAACACTGTTGGG+TGG 0.562616 4_1:-67404653 Msa0566340:CDS
CTTAAATTAAATAAGTGGAT+AGG 0.562680 4_1:-67403998 Msa0566340:three_prime_UTR
AATTAAATAAGTGGATAGGT+GGG 0.568337 4_1:-67403994 Msa0566340:three_prime_UTR
ATCAGCTCACAATGTGGTGG+CGG 0.571374 4_1:-67404313 Msa0566340:CDS
TAGTAGAGAAACATGGCTCT+GGG 0.578678 4_1:-67404771 Msa0566340:exon
TGAGGAGTGAGTGAAGAGGC+AGG 0.584806 4_1:+67404870 None:intergenic
TAAAGAACAGGTACAACAAG+AGG 0.594279 4_1:+67404054 None:intergenic
AATGTGGTGGCGGTGAACAA+AGG 0.599948 4_1:-67404303 Msa0566340:CDS
GTGGTGGCGGTGAACAAAGG+AGG 0.602014 4_1:-67404300 Msa0566340:CDS
TACACTGTTGGAGGTCCTGG+AGG 0.611395 4_1:-67404678 Msa0566340:CDS
CAAATACTAGTAGAGAAACA+TGG 0.613492 4_1:-67404778 Msa0566340:exon
CTAACATATAGTAGCAGACA+TGG 0.615758 4_1:+67403953 None:intergenic
GATAGTTGCAAGACACCAAG+AGG 0.632266 4_1:-67404273 Msa0566340:CDS
TCCATCAGCTCACAATGTGG+TGG 0.645459 4_1:-67404316 Msa0566340:CDS
TGGTGGCGGTGAACAAAGGA+GGG 0.647438 4_1:-67404299 Msa0566340:CDS
TAATTGAGGAGTGAGTGAAG+AGG 0.656376 4_1:+67404866 None:intergenic
CTGCAAGAAATCTTAATGGG+TGG 0.656921 4_1:+67404894 None:intergenic
ACTCATCTGACCAAGACACG+TGG 0.661487 4_1:+67404955 None:intergenic
TTAAGCCAAAATAATAACAG+TGG 0.665899 4_1:+67403822 None:intergenic
GATCAGATCAGGCTTGCTAG+GGG 0.679726 4_1:-67404225 Msa0566340:CDS
TCCAGGACCTCCAACAGTGT+AGG 0.688043 4_1:+67404680 None:intergenic
TAGTCCATCAGCTCACAATG+TGG 0.719459 4_1:-67404319 Msa0566340:CDS
CTCATCTGACCAAGACACGT+GGG 0.723003 4_1:+67404956 None:intergenic
TAAGTGAATTTGGAATGACG+TGG 0.740659 4_1:+67404833 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATATAAACATAATGAGTT+TGG - chr4_1:67403833-67403852 Msa0566340:three_prime_UTR 15.0%
!! AATATAAACATAATGAGTTT+GGG - chr4_1:67403834-67403853 Msa0566340:three_prime_UTR 15.0%
!! TTATCCTTAAATTAAATAAG+TGG - chr4_1:67404814-67404833 Msa0566340:five_prime_UTR 15.0%
!! AAATATAAACATAATGAGTT+TGG - chr4_1:67403833-67403852 Msa0566340:three_prime_UTR 15.0%
!! AATATAAACATAATGAGTTT+GGG - chr4_1:67403834-67403853 Msa0566340:three_prime_UTR 15.0%
!! TTATCCTTAAATTAAATAAG+TGG - chr4_1:67404814-67404833 Msa0566340:five_prime_UTR 15.0%
!! ATAAAATTAAGTACACTCTT+CGG - chr4_1:67404376-67404395 Msa0566340:intron 20.0%
!!! CTATCCACTTATTTAATTTA+AGG + chr4_1:67404821-67404840 None:intergenic 20.0%
!! CTTAAATTAAATAAGTGGAT+AGG - chr4_1:67404819-67404838 Msa0566340:five_prime_UTR 20.0%
!! TAATTGTGTAATATGATTTC+TGG - chr4_1:67404908-67404927 Msa0566340:five_prime_UTR 20.0%
!! AATTGTGTAATATGATTTCT+GGG - chr4_1:67404909-67404928 Msa0566340:five_prime_UTR 20.0%
!!! ATTTACCACTGTTATTATTT+TGG - chr4_1:67404990-67405009 Msa0566340:five_prime_UTR 20.0%
!! ATAAAATTAAGTACACTCTT+CGG - chr4_1:67404376-67404395 Msa0566340:intron 20.0%
!!! CTATCCACTTATTTAATTTA+AGG + chr4_1:67404821-67404840 None:intergenic 20.0%
!! CTTAAATTAAATAAGTGGAT+AGG - chr4_1:67404819-67404838 Msa0566340:five_prime_UTR 20.0%
!! TAATTGTGTAATATGATTTC+TGG - chr4_1:67404908-67404927 Msa0566340:five_prime_UTR 20.0%
!! AATTGTGTAATATGATTTCT+GGG - chr4_1:67404909-67404928 Msa0566340:five_prime_UTR 20.0%
!!! ATTTACCACTGTTATTATTT+TGG - chr4_1:67404990-67405009 Msa0566340:five_prime_UTR 20.0%
! TAAATACTTCTTCATATTGC+AGG + chr4_1:67403891-67403910 None:intergenic 25.0%
! AAGTTGAACACTACATTATT+AGG + chr4_1:67404716-67404735 None:intergenic 25.0%
! AAATTAAATAAGTGGATAGG+TGG - chr4_1:67404822-67404841 Msa0566340:five_prime_UTR 25.0%
! AATTAAATAAGTGGATAGGT+GGG - chr4_1:67404823-67404842 Msa0566340:five_prime_UTR 25.0%
! TTAAGCCAAAATAATAACAG+TGG + chr4_1:67404998-67405017 None:intergenic 25.0%
! TAAATACTTCTTCATATTGC+AGG + chr4_1:67403891-67403910 None:intergenic 25.0%
! AAGTTGAACACTACATTATT+AGG + chr4_1:67404716-67404735 None:intergenic 25.0%
! AAATTAAATAAGTGGATAGG+TGG - chr4_1:67404822-67404841 Msa0566340:five_prime_UTR 25.0%
! AATTAAATAAGTGGATAGGT+GGG - chr4_1:67404823-67404842 Msa0566340:five_prime_UTR 25.0%
! TTAAGCCAAAATAATAACAG+TGG + chr4_1:67404998-67405017 None:intergenic 25.0%
!! GTGGTAAGGTTTTATAATTG+AGG + chr4_1:67403968-67403987 None:intergenic 30.0%
GATTTGAAGGTAAGTGAATT+TGG + chr4_1:67403997-67404016 None:intergenic 30.0%
CTAGAAAACTGTGAAAGAAA+GGG + chr4_1:67404019-67404038 None:intergenic 30.0%
CAAATACTAGTAGAGAAACA+TGG - chr4_1:67404039-67404058 Msa0566340:three_prime_UTR 30.0%
TCTTCAAAACTCTCTTTCAT+CGG - chr4_1:67404353-67404372 Msa0566340:intron 30.0%
TACTTCATTTGCAACTTTGT+GGG - chr4_1:67404622-67404641 Msa0566340:CDS 30.0%
!! ATTGATGGCAATTTTCATTC+CGG + chr4_1:67404659-67404678 None:intergenic 30.0%
! TAAAAGTTAAGCAGCATTGA+TGG + chr4_1:67404674-67404693 None:intergenic 30.0%
! TTCAAGTTCACATAAAGAAC+AGG + chr4_1:67404778-67404797 None:intergenic 30.0%
CTACTATATGTTAGTCATCA+TGG - chr4_1:67404873-67404892 Msa0566340:five_prime_UTR 30.0%
!! GTGGTAAGGTTTTATAATTG+AGG + chr4_1:67403968-67403987 None:intergenic 30.0%
GATTTGAAGGTAAGTGAATT+TGG + chr4_1:67403997-67404016 None:intergenic 30.0%
CTAGAAAACTGTGAAAGAAA+GGG + chr4_1:67404019-67404038 None:intergenic 30.0%
CAAATACTAGTAGAGAAACA+TGG - chr4_1:67404039-67404058 Msa0566340:three_prime_UTR 30.0%
TCTTCAAAACTCTCTTTCAT+CGG - chr4_1:67404353-67404372 Msa0566340:intron 30.0%
TACTTCATTTGCAACTTTGT+GGG - chr4_1:67404622-67404641 Msa0566340:CDS 30.0%
!! ATTGATGGCAATTTTCATTC+CGG + chr4_1:67404659-67404678 None:intergenic 30.0%
! TAAAAGTTAAGCAGCATTGA+TGG + chr4_1:67404674-67404693 None:intergenic 30.0%
! TTCAAGTTCACATAAAGAAC+AGG + chr4_1:67404778-67404797 None:intergenic 30.0%
CTACTATATGTTAGTCATCA+TGG - chr4_1:67404873-67404892 Msa0566340:five_prime_UTR 30.0%
! AAGAAATCTTAATGGGTGGT+TGG + chr4_1:67403922-67403941 None:intergenic 35.0%
! TAAGTGAATTTGGAATGACG+TGG + chr4_1:67403987-67404006 None:intergenic 35.0%
TGTGAAAGAAAGGGATTTGA+AGG + chr4_1:67404010-67404029 None:intergenic 35.0%
GCTAGAAAACTGTGAAAGAA+AGG + chr4_1:67404020-67404039 None:intergenic 35.0%
ACTCCAAACATTACTTCATC+TGG - chr4_1:67404278-67404297 Msa0566340:CDS 35.0%
AGACCAGATGAAGTAATGTT+TGG + chr4_1:67404284-67404303 None:intergenic 35.0%
CTACTTCATTTGCAACTTTG+TGG - chr4_1:67404621-67404640 Msa0566340:CDS 35.0%
TAAAGAACAGGTACAACAAG+AGG + chr4_1:67404766-67404785 None:intergenic 35.0%
CTAACATATAGTAGCAGACA+TGG + chr4_1:67404867-67404886 None:intergenic 35.0%
!!! TAGCGAACCAATTGTTTTGA+AGG - chr4_1:67404949-67404968 Msa0566340:five_prime_UTR 35.0%
!!! AGCGAACCAATTGTTTTGAA+GGG - chr4_1:67404950-67404969 Msa0566340:five_prime_UTR 35.0%
GTAGTTCCCTTCAAAACAAT+TGG + chr4_1:67404959-67404978 None:intergenic 35.0%
! AAGAAATCTTAATGGGTGGT+TGG + chr4_1:67403922-67403941 None:intergenic 35.0%
! TAAGTGAATTTGGAATGACG+TGG + chr4_1:67403987-67404006 None:intergenic 35.0%
TGTGAAAGAAAGGGATTTGA+AGG + chr4_1:67404010-67404029 None:intergenic 35.0%
GCTAGAAAACTGTGAAAGAA+AGG + chr4_1:67404020-67404039 None:intergenic 35.0%
ACTCCAAACATTACTTCATC+TGG - chr4_1:67404278-67404297 Msa0566340:CDS 35.0%
AGACCAGATGAAGTAATGTT+TGG + chr4_1:67404284-67404303 None:intergenic 35.0%
CTACTTCATTTGCAACTTTG+TGG - chr4_1:67404621-67404640 Msa0566340:CDS 35.0%
TAAAGAACAGGTACAACAAG+AGG + chr4_1:67404766-67404785 None:intergenic 35.0%
CTAACATATAGTAGCAGACA+TGG + chr4_1:67404867-67404886 None:intergenic 35.0%
!!! TAGCGAACCAATTGTTTTGA+AGG - chr4_1:67404949-67404968 Msa0566340:five_prime_UTR 35.0%
!!! AGCGAACCAATTGTTTTGAA+GGG - chr4_1:67404950-67404969 Msa0566340:five_prime_UTR 35.0%
GTAGTTCCCTTCAAAACAAT+TGG + chr4_1:67404959-67404978 None:intergenic 35.0%
CTGCAAGAAATCTTAATGGG+TGG + chr4_1:67403926-67403945 None:intergenic 40.0%
CACCCATTAAGATTTCTTGC+AGG - chr4_1:67403924-67403943 Msa0566340:three_prime_UTR 40.0%
GGCCTGCAAGAAATCTTAAT+GGG + chr4_1:67403929-67403948 None:intergenic 40.0%
AGGCCTGCAAGAAATCTTAA+TGG + chr4_1:67403930-67403949 None:intergenic 40.0%
TAATTGAGGAGTGAGTGAAG+AGG + chr4_1:67403954-67403973 None:intergenic 40.0%
! GAATTTGGAATGACGTGGTA+AGG + chr4_1:67403982-67404001 None:intergenic 40.0%
TAGTAGAGAAACATGGCTCT+GGG - chr4_1:67404046-67404065 Msa0566340:three_prime_UTR 40.0%
GTTGGACCTTTAACACTGTT+GGG - chr4_1:67404161-67404180 Msa0566340:CDS 40.0%
GCCTAATGGAAAACGCTTTA+GGG - chr4_1:67404186-67404205 Msa0566340:CDS 40.0%
! CTATACACTTTTGCTCCTCT+TGG + chr4_1:67404562-67404581 None:intergenic 40.0%
! CAAGAGGAGCAAAAGTGTAT+AGG - chr4_1:67404560-67404579 Msa0566340:intron 40.0%
! ATATGATTTCTGGGTCTCTC+AGG - chr4_1:67404918-67404937 Msa0566340:five_prime_UTR 40.0%
CTGCAAGAAATCTTAATGGG+TGG + chr4_1:67403926-67403945 None:intergenic 40.0%
CACCCATTAAGATTTCTTGC+AGG - chr4_1:67403924-67403943 Msa0566340:three_prime_UTR 40.0%
GGCCTGCAAGAAATCTTAAT+GGG + chr4_1:67403929-67403948 None:intergenic 40.0%
AGGCCTGCAAGAAATCTTAA+TGG + chr4_1:67403930-67403949 None:intergenic 40.0%
TAATTGAGGAGTGAGTGAAG+AGG + chr4_1:67403954-67403973 None:intergenic 40.0%
! GAATTTGGAATGACGTGGTA+AGG + chr4_1:67403982-67404001 None:intergenic 40.0%
TAGTAGAGAAACATGGCTCT+GGG - chr4_1:67404046-67404065 Msa0566340:three_prime_UTR 40.0%
GTTGGACCTTTAACACTGTT+GGG - chr4_1:67404161-67404180 Msa0566340:CDS 40.0%
GCCTAATGGAAAACGCTTTA+GGG - chr4_1:67404186-67404205 Msa0566340:CDS 40.0%
! CTATACACTTTTGCTCCTCT+TGG + chr4_1:67404562-67404581 None:intergenic 40.0%
! CAAGAGGAGCAAAAGTGTAT+AGG - chr4_1:67404560-67404579 Msa0566340:intron 40.0%
! ATATGATTTCTGGGTCTCTC+AGG - chr4_1:67404918-67404937 Msa0566340:five_prime_UTR 40.0%
CTAGTAGAGAAACATGGCTC+TGG - chr4_1:67404045-67404064 Msa0566340:three_prime_UTR 45.0%
GGTTGGACCTTTAACACTGT+TGG - chr4_1:67404160-67404179 Msa0566340:CDS 45.0%
GGCCTAATGGAAAACGCTTT+AGG - chr4_1:67404185-67404204 Msa0566340:CDS 45.0%
!! GCCCTAAAGCGTTTTCCATT+AGG + chr4_1:67404190-67404209 None:intergenic 45.0%
!! AATGGAAAACGCTTTAGGGC+TGG - chr4_1:67404190-67404209 Msa0566340:CDS 45.0%
TAGTCCATCAGCTCACAATG+TGG - chr4_1:67404498-67404517 Msa0566340:intron 45.0%
GATAGTTGCAAGACACCAAG+AGG - chr4_1:67404544-67404563 Msa0566340:intron 45.0%
! GGAGCAAAAGTGTATAGGAC+AGG - chr4_1:67404565-67404584 Msa0566340:intron 45.0%
GGACAGGAAAAGATCAGATC+AGG - chr4_1:67404581-67404600 Msa0566340:intron 45.0%
! AAGATCAGATCAGGCTTGCT+AGG - chr4_1:67404590-67404609 Msa0566340:intron 45.0%
!! AGATCAGATCAGGCTTGCTA+GGG - chr4_1:67404591-67404610 Msa0566340:intron 45.0%
CTAGTAGAGAAACATGGCTC+TGG - chr4_1:67404045-67404064 Msa0566340:three_prime_UTR 45.0%
GGTTGGACCTTTAACACTGT+TGG - chr4_1:67404160-67404179 Msa0566340:CDS 45.0%
GGCCTAATGGAAAACGCTTT+AGG - chr4_1:67404185-67404204 Msa0566340:CDS 45.0%
!! GCCCTAAAGCGTTTTCCATT+AGG + chr4_1:67404190-67404209 None:intergenic 45.0%
!! AATGGAAAACGCTTTAGGGC+TGG - chr4_1:67404190-67404209 Msa0566340:CDS 45.0%
TAGTCCATCAGCTCACAATG+TGG - chr4_1:67404498-67404517 Msa0566340:intron 45.0%
GATAGTTGCAAGACACCAAG+AGG - chr4_1:67404544-67404563 Msa0566340:intron 45.0%
! GGAGCAAAAGTGTATAGGAC+AGG - chr4_1:67404565-67404584 Msa0566340:intron 45.0%
GGACAGGAAAAGATCAGATC+AGG - chr4_1:67404581-67404600 Msa0566340:intron 45.0%
! AAGATCAGATCAGGCTTGCT+AGG - chr4_1:67404590-67404609 Msa0566340:intron 45.0%
!! AGATCAGATCAGGCTTGCTA+GGG - chr4_1:67404591-67404610 Msa0566340:intron 45.0%
CTCATCTGACCAAGACACGT+GGG + chr4_1:67403864-67403883 None:intergenic 50.0%
ACTCATCTGACCAAGACACG+TGG + chr4_1:67403865-67403884 None:intergenic 50.0%
!! AGAAACATGGCTCTGGGAAG+AGG - chr4_1:67404052-67404071 Msa0566340:three_prime_UTR 50.0%
CATGCTGCTACCTACACTGT+TGG - chr4_1:67404127-67404146 Msa0566340:three_prime_UTR 50.0%
GTGTTAAAGGTCCAACCTCC+AGG + chr4_1:67404157-67404176 None:intergenic 50.0%
GGACCTTTAACACTGTTGGG+TGG - chr4_1:67404164-67404183 Msa0566340:CDS 50.0%
AGGCCACCCAACAGTGTTAA+AGG + chr4_1:67404170-67404189 None:intergenic 50.0%
AACACTGTTGGGTGGCCTAA+TGG - chr4_1:67404172-67404191 Msa0566340:CDS 50.0%
! TTTAGGGCTGGTGACACACT+TGG - chr4_1:67404202-67404221 Msa0566340:CDS 50.0%
TCCATCAGCTCACAATGTGG+TGG - chr4_1:67404501-67404520 Msa0566340:intron 50.0%
ATCAGCTCACAATGTGGTGG+CGG - chr4_1:67404504-67404523 Msa0566340:intron 50.0%
!! AATGTGGTGGCGGTGAACAA+AGG - chr4_1:67404514-67404533 Msa0566340:intron 50.0%
!! GATCAGATCAGGCTTGCTAG+GGG - chr4_1:67404592-67404611 Msa0566340:intron 50.0%
CTCATCTGACCAAGACACGT+GGG + chr4_1:67403864-67403883 None:intergenic 50.0%
ACTCATCTGACCAAGACACG+TGG + chr4_1:67403865-67403884 None:intergenic 50.0%
!! AGAAACATGGCTCTGGGAAG+AGG - chr4_1:67404052-67404071 Msa0566340:three_prime_UTR 50.0%
CATGCTGCTACCTACACTGT+TGG - chr4_1:67404127-67404146 Msa0566340:three_prime_UTR 50.0%
GTGTTAAAGGTCCAACCTCC+AGG + chr4_1:67404157-67404176 None:intergenic 50.0%
GGACCTTTAACACTGTTGGG+TGG - chr4_1:67404164-67404183 Msa0566340:CDS 50.0%
AGGCCACCCAACAGTGTTAA+AGG + chr4_1:67404170-67404189 None:intergenic 50.0%
AACACTGTTGGGTGGCCTAA+TGG - chr4_1:67404172-67404191 Msa0566340:CDS 50.0%
! TTTAGGGCTGGTGACACACT+TGG - chr4_1:67404202-67404221 Msa0566340:CDS 50.0%
TCCATCAGCTCACAATGTGG+TGG - chr4_1:67404501-67404520 Msa0566340:intron 50.0%
ATCAGCTCACAATGTGGTGG+CGG - chr4_1:67404504-67404523 Msa0566340:intron 50.0%
!! AATGTGGTGGCGGTGAACAA+AGG - chr4_1:67404514-67404533 Msa0566340:intron 50.0%
!! GATCAGATCAGGCTTGCTAG+GGG - chr4_1:67404592-67404611 Msa0566340:intron 50.0%
TGAGGAGTGAGTGAAGAGGC+AGG + chr4_1:67403950-67403969 None:intergenic 55.0%
TCTGGGAAGAGGCAGTGCAT+TGG - chr4_1:67404063-67404082 Msa0566340:three_prime_UTR 55.0%
GCTGCTACCTACACTGTTGG+AGG - chr4_1:67404130-67404149 Msa0566340:three_prime_UTR 55.0%
ACCTACACTGTTGGAGGTCC+TGG - chr4_1:67404136-67404155 Msa0566340:exon 55.0%
TCCAGGACCTCCAACAGTGT+AGG + chr4_1:67404140-67404159 None:intergenic 55.0%
TACACTGTTGGAGGTCCTGG+AGG - chr4_1:67404139-67404158 Msa0566340:exon 55.0%
GCCACCACATTGTGAGCTGA+TGG + chr4_1:67404505-67404524 None:intergenic 55.0%
!! TGGTGGCGGTGAACAAAGGA+GGG - chr4_1:67404518-67404537 Msa0566340:intron 55.0%
TTTGTGGGTCACTGCGAGTC+CGG - chr4_1:67404637-67404656 Msa0566340:CDS 55.0%
TGAGGAGTGAGTGAAGAGGC+AGG + chr4_1:67403950-67403969 None:intergenic 55.0%
TCTGGGAAGAGGCAGTGCAT+TGG - chr4_1:67404063-67404082 Msa0566340:three_prime_UTR 55.0%
GCTGCTACCTACACTGTTGG+AGG - chr4_1:67404130-67404149 Msa0566340:three_prime_UTR 55.0%
ACCTACACTGTTGGAGGTCC+TGG - chr4_1:67404136-67404155 Msa0566340:exon 55.0%
TCCAGGACCTCCAACAGTGT+AGG + chr4_1:67404140-67404159 None:intergenic 55.0%
TACACTGTTGGAGGTCCTGG+AGG - chr4_1:67404139-67404158 Msa0566340:exon 55.0%
GCCACCACATTGTGAGCTGA+TGG + chr4_1:67404505-67404524 None:intergenic 55.0%
!! TGGTGGCGGTGAACAAAGGA+GGG - chr4_1:67404518-67404537 Msa0566340:intron 55.0%
TTTGTGGGTCACTGCGAGTC+CGG - chr4_1:67404637-67404656 Msa0566340:CDS 55.0%
TTGGGCAGACCCACGTGTCT+TGG - chr4_1:67403852-67403871 Msa0566340:three_prime_UTR 60.0%
CTGTTGGAGGTCCTGGAGGT+TGG - chr4_1:67404143-67404162 Msa0566340:exon 60.0%
!! GTGGTGGCGGTGAACAAAGG+AGG - chr4_1:67404517-67404536 Msa0566340:intron 60.0%
TTGGGCAGACCCACGTGTCT+TGG - chr4_1:67403852-67403871 Msa0566340:three_prime_UTR 60.0%
CTGTTGGAGGTCCTGGAGGT+TGG - chr4_1:67404143-67404162 Msa0566340:exon 60.0%
!! GTGGTGGCGGTGAACAAAGG+AGG - chr4_1:67404517-67404536 Msa0566340:intron 60.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 67403821 67405018 67403821 ID=Msa0566340;Name=Msa0566340
chr4_1 mRNA 67403821 67405018 67403821 ID=Msa0566340-mRNA-1;Parent=Msa0566340;Name=Msa0566340-mRNA-1;_AED=0.00;_eAED=0.00;_QI=237|1|1|1|1|1|2|331|121
chr4_1 exon 67403821 67404351 67403821 ID=Msa0566340-mRNA-1:exon:22128;Parent=Msa0566340-mRNA-1
chr4_1 exon 67404616 67405018 67404616 ID=Msa0566340-mRNA-1:exon:22127;Parent=Msa0566340-mRNA-1
chr4_1 five_prime_UTR 67404782 67405018 67404782 ID=Msa0566340-mRNA-1:five_prime_utr;Parent=Msa0566340-mRNA-1
chr4_1 CDS 67404616 67404781 67404616 ID=Msa0566340-mRNA-1:cds;Parent=Msa0566340-mRNA-1
chr4_1 CDS 67404152 67404351 67404152 ID=Msa0566340-mRNA-1:cds;Parent=Msa0566340-mRNA-1
chr4_1 three_prime_UTR 67403821 67404151 67403821 ID=Msa0566340-mRNA-1:three_prime_utr;Parent=Msa0566340-mRNA-1
Gene Sequence

>Msa0566340

ATGGCTCTGGGAAGAGGCAGTGCATTGGTTCTACTAGTCTGCTTTTTTGTTCTTAACTCTGAGCTTGCTCATGCTGCTACCTACACTGTTGGAGGTCCTGGAGGTTGGACCTTTAACACTGTTGGGTGGCCTAATGGAAAACGCTTTAGGGCTGGTGACACACTTGTGTTCAACTATAGTCCATCAGCTCACAATGTGGTGGCGGTGAACAAAGGAGGGTATGATAGTTGCAAGACACCAAGAGGAGCAAAAGTGTATAGGACAGGAAAAGATCAGATCAGGCTTGCTAGGGGACAAAACTACTTCATTTGCAACTTTGTGGGTCACTGCGAGTCCGGAATGAAAATTGCCATCAATGCTGCTTAA
Protein sequence

>Msa0566340

MALGRGSALVLLVCFFVLNSELAHAATYTVGGPGGWTFNTVGWPNGKRFRAGDTLVFNYSPSAHNVVAVNKGGYDSCKTPRGAKVYRTGKDQIRLARGQNYFICNFVGHCESGMKIAINAA*